pycom02g20790

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0006827
Physical Location & Seq
Forward (+)
18808145 .. 18810945
2801 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g20790.1

Sequence Viewer

Length: 1479 bp
ATGCATTCTATAAAGGGTGGTTGGGTTGGGCAGACATTCGCCCTAGCTAAAAATAATGAATCTGAAGGAAGGAGGTCCAGAATTCGGCGTTCAAAGGAAGAGAGGAAGGCAATGGTTGAATCCTTTATAAAAAAGTATCAGAAAATAAACAATGGGAGTTTTCCATCACTTAATCTGACCCACAAAGAAGTTGGTGGGTCTTTCTACACTGTACGAGAAATTGTTCGAGATATAATTCAGGAAAATAGAGTGCTTGGTCCTGCTAAGTTTACTGTAGAGGAGCAGCAGACCATTGATCAGTTCTGGGAACACAATCCATTGGGTACCATTGCTGCAGAACCACAAAATTCTTTGTCAATATCATCAAATGAATTGCTGTTCGTCGCCAATCAAAATCAGGGTGCAATTGAACAACTTGTTTTCGCTTCCGATGGGCATTTAGTTGCACCTGAACATCAGATGTTTGAGAATGGACGAATCATCAATAGTACTCAAATAGAAGTGAATGACAAAGAATTTAATGAACCCAAAAGTACAGAGTTACAAGCAAATGGACCTGTGGAAATAGAGAAGCATGTTGCTGTTGAATCTATAGATTCGAATGGGCATTGTATTGGGCCTGAATATCAGAGTTTTGGCAATGGACTAATCAATGGTAGCCAGATGGATAGGAAAGATAAAGAAAGGGAGGAACGAACATGTACAGAACCACAGACAGTTGAACCTTTGGAAGCAGAGAAGAATGCTGAAGATGTTCAGGCAACATCCAAGTCTAATGTGAATCCTTTAACAGCAGATGTGATAGTGGAGACATTCCCATTGAAGCCGGTAAATGCGACACCGGGCTTGGATGGAAGGTTACAAGAAGTAACGGATTTGACTAAGTCTTCTGAGGACAAGGGAACTAGAGGGTTGGAGTTAGCTGGTGTTGATAGTTCGCAAGTGGATGGATTATATTCCATGAAGAGCATGGATGACAATGTAGCGATGAATCTTTCAAGTCCATTGGTTGAAAACAAATCCAGCCCAGTGGATGAAGCACTAGGAAATGCCATAGATCCATCAGTTGAAAGCTCAAATCACTCAACCTTTAACGAAGGTATTGTGCATGAAACAGGAAGCACAGACCTTAATGTCAAATCACCTCATAAAGATGTTCCAACCTCTGAGATCTCCGACCAAAACCAGCTAACCGCAGGAACTAAGGCTGACAAAGCTCCAGATAGCTTACATACAAAGATTTCCAACGGTACCAGTGGTAGTAGCGAGCAGTCGAAAACCAAAGAGGTGGTTGTGATTGAAGATGAAGTTGATGTCCAGACCAGCGGTAGCTTGAAGAAAGGAAGCAAACCAACTTTGGACAGAATTAATCTTGAATCATGGGAAGGTGAATCCAAAAAGACCAGACCAGAAGGTAACCCACTTTGGGATGTATTTAAAGCGTTTTTAGATGCCTTTGTGAAATTCTGGACTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

493

Amino Acids

54.14

Weight (kDa)

4.91

Isoelectric Point (pI)

42.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HTH_AT3G52170 PF25896 31 - 79 1e-23 Helix-turn-helix domain in AT3G52170-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 128
AasI GACNNNNNNGTC 1 cut(s) 1133
Acc65I GGTACC 2 cut(s) 323, 1250
AccB1I GGYRCC 2 cut(s) 323, 1250
AciI CCGC 2 cut(s) 1194, 1326
AclWI GGATC 1 cut(s) 1052
AcsI RAATTY 4 cut(s) 81, 346, 515, 1463
AcuI CTGAAG 2 cut(s) 84, 768
AfaI GTAC 6 cut(s) 213, 325, 490, 535, 703, 1252
AfiI CCNNNNNNNGG 2 cut(s) 84, 1426
AflIII ACRYGT 1 cut(s) 698
AluBI AGCT 7 cut(s) 47, 923, 1074, 1189, 1217, 1227, 1332
AluI AGCT 7 cut(s) 47, 923, 1074, 1189, 1217, 1227, 1332
Alw26I GTCTC 1 cut(s) 803
AlwI GGATC 1 cut(s) 1052
AoxI GGCC 1 cut(s) 617
ApeKI GCWGC 2 cut(s) 283, 332
ApoI RAATTY 4 cut(s) 81, 346, 515, 1463
AseI ATTAAT 1 cut(s) 1368
Asp700I GAANNNNTTC 2 cut(s) 222, 753
Asp718I GGTACC 2 cut(s) 323, 1250
AspS9I GGNCC 4 cut(s) 75, 257, 554, 617
AsuC2I CCSGG 1 cut(s) 843
AsuHPI GGTGA 2 cut(s) 1134, 1400
AsuII TTCGAA 1 cut(s) 599
AvaII GGWCC 3 cut(s) 75, 257, 554
BanI GGYRCC 2 cut(s) 323, 1250
BbsI GAAGAC 1 cut(s) 879
BbvI GCAGC 2 cut(s) 295, 319
BccI CCATC 6 cut(s) 172, 425, 658, 845, 941, 1069
BclI TGATCA 1 cut(s) 295
BcnI CCSGG 1 cut(s) 843
BcoDI GTCTC 1 cut(s) 803
BfaI CTAG 3 cut(s) 44, 906, 1043
BfmI CTRYAG 3 cut(s) 273, 333, 591
BglII AGATCT 1 cut(s) 1170
BisI GCNGC 2 cut(s) 284, 333
BlsI GCNGC 2 cut(s) 285, 334
BmcAI AGTACT 1 cut(s) 490
Bme1390I CCNGG 1 cut(s) 843
Bme18I GGWCC 3 cut(s) 75, 257, 554
BmgT120I GGNCC 4 cut(s) 75, 257, 554, 617
BmiI GGNNCC 2 cut(s) 325, 1252
BmrFI CCNGG 1 cut(s) 843
BmrI ACTGGG 1 cut(s) 1022
BmsI GCATC 1 cut(s) 1441
BmuI ACTGGG 1 cut(s) 1022
BpiI GAAGAC 1 cut(s) 879
BpmI CTGGAG 1 cut(s) 1203
Bpu14I TTCGAA 1 cut(s) 599
BpuMI CCSGG 1 cut(s) 843
Bsc4I CCNNNNNNNGG 2 cut(s) 84, 1426
Bse118I RCCGGY 1 cut(s) 826
Bse1I ACTGG 2 cut(s) 1028, 1254
Bse3DI GCAATG 3 cut(s) 117, 327, 646
BseGI GGATG 6 cut(s) 764, 856, 952, 979, 1039, 1435
BseLI CCNNNNNNNGG 2 cut(s) 84, 1426
BseMI GCAATG 3 cut(s) 117, 327, 646
BseMII CTCAG 2 cut(s) 882, 1158
BseNI ACTGG 2 cut(s) 1028, 1254
BseRI GAGGAG 1 cut(s) 293
BseXI GCAGC 2 cut(s) 295, 319
BshFI GGCC 1 cut(s) 619
BshNI GGYRCC 2 cut(s) 323, 1250
BsiSI CCGG 2 cut(s) 827, 842
BslI CCNNNNNNNGG 2 cut(s) 84, 1426
BsmAI GTCTC 1 cut(s) 803
BsmI GAATGC 2 cut(s) 4, 748
BsnI GGCC 1 cut(s) 619
Bsp119I TTCGAA 1 cut(s) 599
Bsp1407I TGTACA 1 cut(s) 701
Bsp143I GATC 3 cut(s) 295, 1057, 1170
BspACI CCGC 2 cut(s) 1194, 1326
BspANI GGCC 1 cut(s) 619
BspCNI CTCAG 2 cut(s) 883, 1159
BspLI GGNNCC 2 cut(s) 325, 1252
BspMAI CTGCAG 1 cut(s) 337
BspPI GGATC 1 cut(s) 1052
BspQI GCTCTTC 1 cut(s) 959
BspT104I TTCGAA 1 cut(s) 599
BspT107I GGYRCC 2 cut(s) 323, 1250
BsrDI GCAATG 3 cut(s) 117, 327, 646
BsrFI RCCGGY 1 cut(s) 826
BsrGI TGTACA 1 cut(s) 701
BsrI ACTGG 2 cut(s) 1028, 1254
BssAI RCCGGY 1 cut(s) 826
BssMI GATC 3 cut(s) 295, 1057, 1170
Bst4CI ACNGT 4 cut(s) 211, 274, 718, 1250
Bst6I CTCTTC 2 cut(s) 93, 959
BstAUI TGTACA 1 cut(s) 701
BstBI TTCGAA 1 cut(s) 599
BstC8I GCNNGC 1 cut(s) 1268
BstDEI CTNAG 5 cut(s) 264, 882, 891, 1167, 1203
BstEII GGTNACC 1 cut(s) 1415
BstF5I GGATG 6 cut(s) 764, 856, 952, 979, 1039, 1435
BstKTI GATC 3 cut(s) 298, 1060, 1173
BstMAI GTCTC 1 cut(s) 803
BstMBI GATC 3 cut(s) 295, 1057, 1170
BstMWI GCNNNNNNNGC 1 cut(s) 1214
BstNSI RCATGY 2 cut(s) 578, 702
BstPI GGTNACC 1 cut(s) 1415
BstSCI CCNGG 1 cut(s) 841
BstSFI CTRYAG 3 cut(s) 273, 333, 591
BstV1I GCAGC 2 cut(s) 295, 319
BstV2I GAAGAC 1 cut(s) 879
BstX2I RGATCY 2 cut(s) 1057, 1170
BstXI CCANNNNNNTGG 2 cut(s) 1030, 1288
BstYI RGATCY 2 cut(s) 1057, 1170
BsuRI GGCC 1 cut(s) 619
BtgZI GCGATG 1 cut(s) 1001
BtsCI GGATG 6 cut(s) 764, 856, 952, 979, 1039, 1435
BtsIMutI CAGTG 3 cut(s) 207, 1035, 1261
Cac8I GCNNGC 1 cut(s) 1268
Cfr10I RCCGGY 1 cut(s) 826
Cfr13I GGNCC 4 cut(s) 75, 257, 554, 617
Csp6I GTAC 6 cut(s) 212, 324, 489, 534, 702, 1251
CviAII CATG 6 cut(s) 575, 699, 961, 970, 1109, 1380
CviQI GTAC 6 cut(s) 212, 324, 489, 534, 702, 1251
DdeI CTNAG 5 cut(s) 264, 882, 891, 1167, 1203
DpnI GATC 3 cut(s) 297, 1059, 1172
DpnII GATC 3 cut(s) 295, 1057, 1170
DraI TTTAAA 1 cut(s) 1438
DrdI GACNNNNNNGTC 1 cut(s) 1133
DseDI GACNNNNNNGTC 1 cut(s) 1133
Eam1104I CTCTTC 2 cut(s) 93, 959
EarI CTCTTC 2 cut(s) 93, 959
Eco47I GGWCC 3 cut(s) 75, 257, 554
Eco57I CTGAAG 2 cut(s) 84, 768
Eco91I GGTNACC 1 cut(s) 1415
EcoO65I GGTNACC 1 cut(s) 1415
EcoRI GAATTC 1 cut(s) 81
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 6 cut(s) 578, 702, 964, 973, 1112, 1383
FatI CATG 6 cut(s) 574, 698, 960, 969, 1108, 1379
FbaI TGATCA 1 cut(s) 295
Fnu4HI GCNGC 2 cut(s) 284, 333
FokI GGATG 6 cut(s) 751, 863, 959, 986, 1046, 1442
Fsp4HI GCNGC 2 cut(s) 284, 333
FspBI CTAG 3 cut(s) 44, 906, 1043
GluI GCNGC 2 cut(s) 284, 333
GsuI CTGGAG 1 cut(s) 1203
HaeIII GGCC 1 cut(s) 619
HapII CCGG 2 cut(s) 827, 842
Hin1II CATG 6 cut(s) 578, 702, 964, 973, 1112, 1383
HinfI GANTC 9 cut(s) 59, 119, 477, 587, 596, 781, 991, 1376, 1391
HpaII CCGG 2 cut(s) 827, 842
HphI GGTGA 2 cut(s) 1134, 1400
Hpy166II GTNNAC 1 cut(s) 270
Hpy188I TCNGA 9 cut(s) 64, 141, 177, 430, 459, 630, 892, 1168, 1177
Hpy188III TCNNGA 7 cut(s) 78, 227, 239, 1220, 1318, 1373, 1468
Hpy8I GTNNAC 1 cut(s) 270
Hpy99I CGWCG 1 cut(s) 386
HpyAV CCTTC 7 cut(s) 59, 63, 100, 849, 1091, 1379, 1406
HpyCH4III ACNGT 4 cut(s) 211, 274, 718, 1250
HpyCH4V TGCA 5 cut(s) 4, 335, 404, 446, 1108
HpyF10VI GCNNNNNNNGC 1 cut(s) 1214
HpyF3I CTNAG 5 cut(s) 264, 882, 891, 1167, 1203
Hsp92II CATG 6 cut(s) 578, 702, 964, 973, 1112, 1383
KpnI GGTACC 2 cut(s) 327, 1254
Ksp22I TGATCA 1 cut(s) 295
Kzo9I GATC 3 cut(s) 295, 1057, 1170
LguI GCTCTTC 1 cut(s) 959
LmnI GCTCC 2 cut(s) 280, 1222
Lsp1109I GCAGC 2 cut(s) 295, 319
LweI GCATC 1 cut(s) 1441
MaeI CTAG 3 cut(s) 44, 906, 1043
MaeIII GTNAC 4 cut(s) 540, 858, 868, 1415
MalI GATC 3 cut(s) 297, 1059, 1172
MboI GATC 3 cut(s) 295, 1057, 1170
MboII GAAGA 7 cut(s) 110, 751, 761, 879, 976, 1313, 1348
MfeI CAATTG 1 cut(s) 405
MflI RGATCY 2 cut(s) 1057, 1170
MluCI AATT 9 cut(s) 81, 219, 234, 346, 371, 405, 515, 1365, 1463
MmeI TCCRAC 4 cut(s) 894, 1184, 1200, 1269
MnlI CCTC 9 cut(s) 66, 96, 271, 682, 886, 902, 1155, 1174, 1279
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 222, 753
MseI TTAA 7 cut(s) 171, 519, 788, 1092, 1131, 1368, 1437
MslI CAYNNNNRTG 1 cut(s) 1152
MspA1I CMGCKG 1 cut(s) 1326
MspI CCGG 2 cut(s) 827, 842
MspR9I CCNGG 1 cut(s) 843
MunI CAATTG 1 cut(s) 405
Mva1269I GAATGC 2 cut(s) 4, 748
MwoI GCNNNNNNNGC 1 cut(s) 1214
NciI CCSGG 1 cut(s) 843
NdeII GATC 3 cut(s) 295, 1057, 1170
NlaIII CATG 6 cut(s) 578, 702, 964, 973, 1112, 1383
NlaIV GGNNCC 2 cut(s) 325, 1252
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 578, 702
NspV TTCGAA 1 cut(s) 599
PciI ACATGT 1 cut(s) 698
PciSI GCTCTTC 1 cut(s) 959
PctI GAATGC 2 cut(s) 4, 748
PdmI GAANNNNTTC 2 cut(s) 222, 753
PfeI GAWTC 9 cut(s) 59, 119, 477, 587, 596, 781, 991, 1376, 1391
PflFI GACNNNGTC 1 cut(s) 883
PkrI GCNGC 2 cut(s) 285, 334
PscI ACATGT 1 cut(s) 698
PshBI ATTAAT 1 cut(s) 1368
PsiI TTATAA 1 cut(s) 128
PspEI GGTNACC 1 cut(s) 1415
PspN4I GGNNCC 2 cut(s) 325, 1252
PspPI GGNCC 4 cut(s) 75, 257, 554, 617
PstI CTGCAG 1 cut(s) 337
PsuI RGATCY 2 cut(s) 1057, 1170
PsyI GACNNNGTC 1 cut(s) 883
RsaI GTAC 6 cut(s) 213, 325, 490, 535, 703, 1252
RsaNI GTAC 6 cut(s) 212, 324, 489, 534, 702, 1251
RseI CAYNNNNRTG 1 cut(s) 1152
SapI GCTCTTC 1 cut(s) 959
SaqAI TTAA 7 cut(s) 171, 519, 788, 1092, 1131, 1368, 1437
SatI GCNGC 2 cut(s) 284, 333
Sau3AI GATC 3 cut(s) 295, 1057, 1170
Sau96I GGNCC 4 cut(s) 75, 257, 554, 617
ScaI AGTACT 1 cut(s) 490
ScrFI CCNGG 1 cut(s) 843
SfaNI GCATC 1 cut(s) 1441
SfcI CTRYAG 3 cut(s) 273, 333, 591
SfuI TTCGAA 1 cut(s) 599
SinI GGWCC 3 cut(s) 75, 257, 554
SmiMI CAYNNNNRTG 1 cut(s) 1152
Sse9I AATT 9 cut(s) 81, 219, 234, 346, 371, 405, 515, 1365, 1463
SsiI CCGC 2 cut(s) 1194, 1326
SspMI CTAG 3 cut(s) 44, 906, 1043
StyD4I CCNGG 1 cut(s) 841
TaaI ACNGT 4 cut(s) 211, 274, 718, 1250
TaqI TCGA 3 cut(s) 226, 599, 1274
TasI AATT 9 cut(s) 81, 219, 234, 346, 371, 405, 515, 1365, 1463
TatI WGTACW 3 cut(s) 488, 533, 701
TfiI GAWTC 9 cut(s) 59, 119, 477, 587, 596, 781, 991, 1376, 1391
Tru1I TTAA 7 cut(s) 171, 519, 788, 1092, 1131, 1368, 1437
Tru9I TTAA 7 cut(s) 171, 519, 788, 1092, 1131, 1368, 1437
TscAI CASTG 3 cut(s) 214, 1035, 1261
TseI GCWGC 2 cut(s) 283, 332
TspDTI ATGAA 8 cut(s) 72, 384, 537, 977, 1004, 1050, 1125, 1320
TspGWI ACGGA 1 cut(s) 887
TspRI CASTG 3 cut(s) 214, 1035, 1261
Tth111I GACNNNGTC 1 cut(s) 883
VpaK11BI GGWCC 3 cut(s) 75, 257, 554
VspI ATTAAT 1 cut(s) 1368
XapI RAATTY 4 cut(s) 81, 346, 515, 1463
XceI RCATGY 2 cut(s) 578, 702
XcmI CCANNNNNNNNNTGG 2 cut(s) 188, 967
XmnI GAANNNNTTC 2 cut(s) 222, 753
XspI CTAG 3 cut(s) 44, 906, 1043
ZrmI AGTACT 1 cut(s) 490
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.