Rh1DG153400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0006827
Physical Location & Seq
Reverse (-)
32133261 .. 32137660
4400 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG153400.1

Sequence Viewer

Length: 1203 bp
ATGCATGCTATAAAGGGTGGCTGGGTTGGGCAAACATTTGCCCTAGCTAGAAACAATGAATCTGAAGGAAGGAAGTCCCGAATTCGGCGTTCAAAGGAGGAGAGGAAGGAAATGGTTGAAGCCTTTATAAAAAAGTATCAGATTCTACACAACGGGAGTTTTCCATCACTTAATCTGACCCACAAGGAAGTTGGTGGATCTTTCTACACTGTACGAGAAATTGTCCGAGATGTAATCCAAGAAAATAGAGTGCTGGGTCCTGCTAAGTTCGTTGCAGAGGATCAGACTATTGATCAGTTCTTGGAACGTAATCCACTTGGTTCGATTGCTGCAGAACCTCAAAATGCTTTGTCAGAAGCATCAAATGATTCTCAGTTTGCCAACAATCAAAATCAGGGTACAGTTCAAGAAATGGTTTTGGCTTCTGATGGTCATTCGTTTACTCTTGAACATCAGATTCTCGACCATGGCCGAGCCATCAATGGCACTGCAGCAGAAGTGAATAGCAAAGAAGTGGAGAGTAAAGAGTTGCAAGTAACTGTGCCAGTGGAAACAGAGAAGAAGGTAGCTGAAGAATCTGTAGTTTCTGATGGGAATTGTATTTGTCATGAATATCAGATGTTTGACAATGGCGGCCAGGTAGATCAGGAAGACAAACAAACTGAGGAACTGATATGTGCAGAGCATAGAACAAGTGTGCCTTTGGAAGTAGAGAAAAATGTTGAAGAAGTTTTGGCAATATCTAGGTCTAAAGTGACCTCCATAGAAGCAGATGTAATAGTTGAGACATTCCCCTTAACGCCTGTTACTAGGACTACTGAGAGCTTGGATGGAAAGGTAGAATTAGGAAATTTTGGTATATCTACTGAGGATAAAGGAACCAAGGGGATGGGGTTAGCCACTGGTGTTGATAGTACGCTGTCGGATACAGTATATTCCGTGAATAGCTTGGTCGATGATAAAGTAGTGGTGAAAAGCCCCTTGGTTGGAAACAAATCCAGCTCAGTAAATGAGGAAGCCTTAGAAATTGTCAGAGACCCATCACTGGAGACCTCAAATCGCTCAACCCTTGAAGGAAATATTATACATGAAAAAGGAAGCACAGACCTTAAGGTCAAAGCACCTGGAAATGATGTTCCTATATCTGAGAGCTTTGAACAAATTGAAGGGACTGCTGGAGCTAAGGTATTTTTTTTATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

400

Amino Acids

43.8

Weight (kDa)

4.82

Isoelectric Point (pI)

39.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HTH_AT3G52170 PF25896 31 - 79 3.6e-22 Helix-turn-helix domain in AT3G52170-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 128
AasI GACNNNNNNGTC 1 cut(s) 1112
AciI CCGC 1 cut(s) 633
AclWI GGATC 2 cut(s) 205, 288
AcoI YGGCCR 2 cut(s) 469, 634
AcsI RAATTY 2 cut(s) 81, 850
AcuI CTGAAG 2 cut(s) 84, 591
AfaI GTAC 3 cut(s) 213, 400, 916
AfiI CCNNNNNNNGG 3 cut(s) 84, 986, 1045
AflII CTTAAG 1 cut(s) 1109
AgsI TTSAA 8 cut(s) 93, 119, 407, 449, 725, 1073, 1157, 1166
AjnI CCWGG 2 cut(s) 636, 1123
AjuI GAANNNNNNNTTGG 2 cut(s) 965, 997
AloI GAACNNNNNNTCC 2 cut(s) 1119, 1151
AluBI AGCT 7 cut(s) 47, 569, 825, 948, 1002, 1152, 1181
AluI AGCT 7 cut(s) 47, 569, 825, 948, 1002, 1152, 1181
Alw26I GTCTC 3 cut(s) 779, 1029, 1043
AlwI GGATC 2 cut(s) 205, 288
AoxI GGCC 2 cut(s) 469, 634
ApeKI GCWGC 2 cut(s) 329, 491
ApoI RAATTY 2 cut(s) 81, 850
AspS9I GGNCC 1 cut(s) 257
AsuHPI GGTGA 1 cut(s) 982
AvaII GGWCC 1 cut(s) 257
BbsI GAAGAC 1 cut(s) 657
BbvI GCAGC 2 cut(s) 316, 503
BccI CCATC 7 cut(s) 172, 422, 485, 584, 824, 883, 1048
BciT130I CCWGG 2 cut(s) 638, 1125
BciVI GTATCC 1 cut(s) 919
BclI TGATCA 1 cut(s) 292
BcoDI GTCTC 3 cut(s) 779, 1029, 1043
BfaI CTAG 4 cut(s) 44, 48, 744, 810
BfmI CTRYAG 3 cut(s) 330, 489, 579
BfrI CTTAAG 1 cut(s) 1109
BfuI GTATCC 1 cut(s) 919
BisI GCNGC 3 cut(s) 330, 492, 634
BlsI GCNGC 3 cut(s) 331, 493, 635
Bme1390I CCNGG 2 cut(s) 638, 1125
Bme18I GGWCC 1 cut(s) 257
BmgT120I GGNCC 1 cut(s) 257
BmiI GGNNCC 2 cut(s) 258, 880
BmrFI CCNGG 2 cut(s) 638, 1125
BmsI GCATC 1 cut(s) 368
BpiI GAAGAC 1 cut(s) 657
BpmI CTGGAG 2 cut(s) 1067, 1197
Bpu10I CCTNAGC 1 cut(s) 1182
BsaI GGTCTC 2 cut(s) 1029, 1043
BsaJI CCNNGG 3 cut(s) 466, 882, 981
BsaXI ACNNNNNCTCC 2 cut(s) 1170, 1200
Bsc4I CCNNNNNNNGG 3 cut(s) 84, 986, 1045
Bse1I ACTGG 3 cut(s) 545, 907, 1050
BseBI CCWGG 2 cut(s) 638, 1125
BseDI CCNNGG 3 cut(s) 466, 882, 981
BseGI GGATG 2 cut(s) 835, 894
BseLI CCNNNNNNNGG 3 cut(s) 84, 986, 1045
BseMII CTCAG 6 cut(s) 386, 654, 810, 858, 1017, 1137
BseNI ACTGG 3 cut(s) 545, 907, 1050
BseRI GAGGAG 1 cut(s) 113
BseXI GCAGC 2 cut(s) 316, 503
BseYI CCCAGC 2 cut(s) 21, 253
BsgI GTGCAG 1 cut(s) 699
BshFI GGCC 2 cut(s) 471, 636
BslFI GGGAC 2 cut(s) 61, 1183
BslI CCNNNNNNNGG 3 cut(s) 84, 986, 1045
BsmAI GTCTC 3 cut(s) 779, 1029, 1043
BsmFI GGGAC 2 cut(s) 61, 1183
BsnI GGCC 2 cut(s) 471, 636
Bso31I GGTCTC 2 cut(s) 1029, 1043
Bsp143I GATC 4 cut(s) 197, 280, 292, 643
Bsp19I CCATGG 1 cut(s) 466
BspACI CCGC 1 cut(s) 633
BspANI GGCC 2 cut(s) 471, 636
BspCNI CTCAG 6 cut(s) 385, 655, 811, 859, 1016, 1138
BspHI TCATGA 1 cut(s) 607
BspLI GGNNCC 2 cut(s) 258, 880
BspMAI CTGCAG 2 cut(s) 334, 493
BspPI GGATC 2 cut(s) 205, 288
BspTI CTTAAG 1 cut(s) 1109
BspTNI GGTCTC 2 cut(s) 1029, 1043
BsrI ACTGG 3 cut(s) 545, 907, 1050
BssECI CCNNGG 3 cut(s) 466, 882, 981
BssMI GATC 4 cut(s) 197, 280, 292, 643
BssT1I CCWWGG 3 cut(s) 466, 882, 981
Bst2UI CCWGG 2 cut(s) 638, 1125
Bst4CI ACNGT 4 cut(s) 211, 403, 541, 931
BstAFI CTTAAG 1 cut(s) 1109
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 9 cut(s) 264, 372, 663, 819, 867, 1003, 1021, 1146, 1182
BstDSI CCRYGG 1 cut(s) 466
BstF5I GGATG 2 cut(s) 835, 894
BstKTI GATC 4 cut(s) 200, 283, 295, 646
BstMAI GTCTC 3 cut(s) 779, 1029, 1043
BstMBI GATC 4 cut(s) 197, 280, 292, 643
BstNI CCWGG 2 cut(s) 638, 1125
BstNSI RCATGY 1 cut(s) 8
BstSCI CCNGG 2 cut(s) 636, 1123
BstSFI CTRYAG 3 cut(s) 330, 489, 579
BstV1I GCAGC 2 cut(s) 316, 503
BstV2I GAAGAC 1 cut(s) 657
BstX2I RGATCY 1 cut(s) 197
BstXI CCANNNNNNTGG 1 cut(s) 889
BstYI RGATCY 1 cut(s) 197
BsuI GTATCC 1 cut(s) 919
BsuRI GGCC 2 cut(s) 471, 636
BtgI CCRYGG 1 cut(s) 466
BtsCI GGATG 2 cut(s) 835, 894
BtsI GCAGTG 1 cut(s) 486
BtsIMutI CAGTG 5 cut(s) 207, 486, 552, 900, 1043
Cac8I GCNNGC 1 cut(s) 6
CciI TCATGA 1 cut(s) 607
Cfr13I GGNCC 1 cut(s) 257
Csp6I GTAC 3 cut(s) 212, 399, 915
CspCI CAANNNNNGTGG 2 cut(s) 889, 924
CviAII CATG 4 cut(s) 5, 467, 608, 1088
CviQI GTAC 3 cut(s) 212, 399, 915
DdeI CTNAG 9 cut(s) 264, 372, 663, 819, 867, 1003, 1021, 1146, 1182
DpnI GATC 4 cut(s) 199, 282, 294, 645
DpnII GATC 4 cut(s) 197, 280, 292, 643
DrdI GACNNNNNNGTC 1 cut(s) 1112
DseDI GACNNNNNNGTC 1 cut(s) 1112
EaeI YGGCCR 2 cut(s) 469, 634
Eco130I CCWWGG 3 cut(s) 466, 882, 981
Eco31I GGTCTC 2 cut(s) 1029, 1043
Eco47I GGWCC 1 cut(s) 257
Eco57I CTGAAG 2 cut(s) 84, 591
EcoO109I RGGNCCY 1 cut(s) 257
EcoRI GAATTC 1 cut(s) 81
EcoRII CCWGG 2 cut(s) 636, 1123
EcoT14I CCWWGG 3 cut(s) 466, 882, 981
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 3 cut(s) 466, 882, 981
FaeI CATG 4 cut(s) 8, 470, 611, 1091
FalI AAGNNNNNCTT 2 cut(s) 685, 717
FaqI GGGAC 2 cut(s) 61, 1183
FatI CATG 4 cut(s) 4, 466, 607, 1087
FbaI TGATCA 1 cut(s) 292
Fnu4HI GCNGC 3 cut(s) 330, 492, 634
FokI GGATG 2 cut(s) 842, 901
Fsp4HI GCNGC 3 cut(s) 330, 492, 634
FspBI CTAG 4 cut(s) 44, 48, 744, 810
GluI GCNGC 3 cut(s) 330, 492, 634
GsaI CCCAGC 2 cut(s) 25, 257
GsuI CTGGAG 2 cut(s) 1067, 1197
HaeIII GGCC 2 cut(s) 471, 636
Hin1II CATG 4 cut(s) 8, 470, 611, 1091
HinfI GANTC 5 cut(s) 59, 142, 368, 457, 575
HphI GGTGA 1 cut(s) 982
Hpy166II GTNNAC 1 cut(s) 441
Hpy188III TCNNGA 6 cut(s) 78, 407, 446, 461, 608, 647
Hpy8I GTNNAC 1 cut(s) 441
HpyAV CCTTC 6 cut(s) 59, 63, 100, 556, 1067, 1160
HpyCH4III ACNGT 4 cut(s) 211, 403, 541, 931
HpyCH4IV ACGT 1 cut(s) 307
HpyCH4V TGCA 6 cut(s) 4, 275, 332, 491, 532, 680
HpyF3I CTNAG 9 cut(s) 264, 372, 663, 819, 867, 1003, 1021, 1146, 1182
HpySE526I ACGT 1 cut(s) 307
Hsp92II CATG 4 cut(s) 8, 470, 611, 1091
Ksp22I TGATCA 1 cut(s) 292
Kzo9I GATC 4 cut(s) 197, 280, 292, 643
LmnI GCTCC 1 cut(s) 1178
Lsp1109I GCAGC 2 cut(s) 316, 503
LweI GCATC 1 cut(s) 368
MaeI CTAG 4 cut(s) 44, 48, 744, 810
MaeII ACGT 1 cut(s) 307
MaeIII GTNAC 3 cut(s) 535, 754, 805
MalI GATC 4 cut(s) 199, 282, 294, 645
MboI GATC 4 cut(s) 197, 280, 292, 643
MboII GAAGA 4 cut(s) 571, 584, 662, 737
MflI RGATCY 1 cut(s) 197
MluCI AATT 7 cut(s) 81, 219, 595, 842, 850, 1026, 1161
MmeI TCCRAC 2 cut(s) 903, 967
MnlI CCTC 9 cut(s) 91, 96, 271, 348, 658, 769, 862, 1006, 1063
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 3 cut(s) 171, 797, 1110
MspCI CTTAAG 1 cut(s) 1109
MspR9I CCNGG 2 cut(s) 638, 1125
MvaI CCWGG 2 cut(s) 638, 1125
NcoI CCATGG 1 cut(s) 466
NdeII GATC 4 cut(s) 197, 280, 292, 643
NlaIII CATG 4 cut(s) 8, 470, 611, 1091
NlaIV GGNNCC 2 cut(s) 258, 880
NmeAIII GCCGAG 1 cut(s) 497
NmuCI GTSAC 1 cut(s) 754
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 8
PaeI GCATGC 1 cut(s) 8
PagI TCATGA 1 cut(s) 607
PfeI GAWTC 5 cut(s) 59, 142, 368, 457, 575
PkrI GCNGC 3 cut(s) 331, 493, 635
PpuMI RGGWCCY 1 cut(s) 257
PsiI TTATAA 1 cut(s) 128
Psp5II RGGWCCY 1 cut(s) 257
Psp6I CCWGG 2 cut(s) 636, 1123
PspFI CCCAGC 2 cut(s) 21, 253
PspGI CCWGG 2 cut(s) 636, 1123
PspN4I GGNNCC 2 cut(s) 258, 880
PspPI GGNCC 1 cut(s) 257
PspPPI RGGWCCY 1 cut(s) 257
PstI CTGCAG 2 cut(s) 334, 493
PsuI RGATCY 1 cut(s) 197
RsaI GTAC 3 cut(s) 213, 400, 916
RsaNI GTAC 3 cut(s) 212, 399, 915
SaqAI TTAA 3 cut(s) 171, 797, 1110
SatI GCNGC 3 cut(s) 330, 492, 634
Sau3AI GATC 4 cut(s) 197, 280, 292, 643
Sau96I GGNCC 1 cut(s) 257
ScrFI CCNGG 2 cut(s) 638, 1125
SfaNI GCATC 1 cut(s) 368
SfcI CTRYAG 3 cut(s) 330, 489, 579
SinI GGWCC 1 cut(s) 257
SmlI CTYRAG 1 cut(s) 1109
SmoI CTYRAG 1 cut(s) 1109
SphI GCATGC 1 cut(s) 8
Sse9I AATT 7 cut(s) 81, 219, 595, 842, 850, 1026, 1161
SsiI CCGC 1 cut(s) 633
SspI AATATT 1 cut(s) 1081
SspMI CTAG 4 cut(s) 44, 48, 744, 810
StyD4I CCNGG 2 cut(s) 636, 1123
StyI CCWWGG 3 cut(s) 466, 882, 981
TaaI ACNGT 4 cut(s) 211, 403, 541, 931
TaiI ACGT 1 cut(s) 310
TaqI TCGA 3 cut(s) 323, 462, 954
TasI AATT 7 cut(s) 81, 219, 595, 842, 850, 1026, 1161
TauI GCSGC 1 cut(s) 636
TfiI GAWTC 5 cut(s) 59, 142, 368, 457, 575
Tru1I TTAA 3 cut(s) 171, 797, 1110
Tru9I TTAA 3 cut(s) 171, 797, 1110
TscAI CASTG 5 cut(s) 214, 493, 552, 907, 1050
TseFI GTSAC 1 cut(s) 754
TseI GCWGC 2 cut(s) 329, 491
Tsp45I GTSAC 1 cut(s) 754
TspDTI ATGAA 3 cut(s) 72, 624, 1104
TspGWI ACGGA 1 cut(s) 928
TspRI CASTG 5 cut(s) 214, 493, 552, 907, 1050
Vha464I CTTAAG 1 cut(s) 1109
VpaK11BI GGWCC 1 cut(s) 257
XapI RAATTY 2 cut(s) 81, 850
XceI RCATGY 1 cut(s) 8
XcmI CCANNNNNNNNNTGG 1 cut(s) 188
XspI CTAG 4 cut(s) 44, 48, 744, 810
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.