MD02G1258700.v1.1

Belongs to the short-chain dehydrogenases reductases (SDR) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008576
Physical Location & Seq
Forward (+)
31195073 .. 31196862
1790 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1258700.v1.1.491

Sequence Viewer

Length: 876 bp
ATGGCGGTGGATCGACATCGGAGAAGATGGTGGTCGGAGGACACGGTCGCGATAGTGACCGGAGCAAACAAAGGAATAGGGTTTGCAGTTGTGAAGAGGATGGCGGAGTTGGGAGTGACGGTGATATTAACAGCCAGAGACAAGGAAAGGGGTTGCAAGGCTGTTGCGGCACTCAGAGCTCAAGGTCTTCATAATGTAACCTTCTCTTGCCTTGATGTCTCCGATCCTTCTTCTATCAAATCTTTCACTTCATGGTTCAAAAAAAGCTATGCAGTACTGGATATTCTTGTGAACAATGCAGCTGTGTCTTTTAATGATATCAATGAAAACTCGGTGGAACATGCCGAGATAGTGATGAAAACCAACTTCTATGGACCTAAGTTACTAACTGATGCCCTCTTGCCCATGTTCCATCATAGCACCTCCTCCGTTGGTCGGATACTTAACGTTAGCTCCAGACTCGGCTCACTAAATAAGCTAAGAAACCCTAGCACAAAACGAGTTCTAGAAAGCGAAACACTGTCGGAAAATGATATCGAGGGGGTGGTTAGTTTGTTTCTTGAAGACGTCAAGAGCGGCAGATGGAAGAGCCAAGGGTGGCCTAAAGTATGGACCGACTATGCTGTGTCAAAGCTTGCACTCAATTCATACACAAGAGTTTTGGCCAAGCGATATGAGGGGAGGGGTCTGAGTGTGAATAGCTTCTGTCCCGGCTTCACGCAGACATCTATGACTCGTTGTAAGGGCGACCACACAGCCGATGATGCCGCTAGTGTTGGCGCAAGGCTGGCATTGCTTCCTCCTGAGGAGATTCCAACGGGGAAATTTTTTTTGTGGGTTAGTACTAGTGGTAATGTCATACCCTCTAAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00590 ko00790 ko00980 ko01100 ko05204 map00590 map00790 map00980 map01100 map05204
Pfam Domains
Protein Families
adh_short

Protein Analysis

292

Amino Acids

32.06

Weight (kDa)

9.43

Isoelectric Point (pI)

37.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 16 - 156 7.8e-29 short chain dehydrogenase
KR PF08659 17 - 135 4.1e-07 KR domain
adh_short_C2 PF13561 21 - 153 1.2e-20 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 198 - 249 5.4e-07 Enoyl-(Acyl carrier protein) reductase
adh_short PF00106 203 - 248 2.6e-07 short chain dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 570
AccBSI CCGCTC 1 cut(s) 576
AccII CGCG 1 cut(s) 50
AciI CCGC 5 cut(s) 5, 104, 167, 576, 768
AclI AACGTT 1 cut(s) 447
AclWI GGATC 2 cut(s) 18, 218
AcoI YGGCCR 1 cut(s) 663
AcsI RAATTY 1 cut(s) 824
AcyI GRCGYC 1 cut(s) 567
AfaI GTAC 2 cut(s) 276, 844
AfiI CCNNNNNNNGG 1 cut(s) 435
AgsI TTSAA 2 cut(s) 259, 563
AhlI ACTAGT 1 cut(s) 845
AluBI AGCT 7 cut(s) 179, 267, 302, 453, 478, 634, 702
AluI AGCT 7 cut(s) 179, 267, 302, 453, 478, 634, 702
Alw21I GWGCWC 1 cut(s) 181
Alw26I GTCTC 2 cut(s) 132, 223
AlwI GGATC 2 cut(s) 18, 218
AoxI GGCC 2 cut(s) 599, 663
ApeKI GCWGC 1 cut(s) 299
ApoI RAATTY 1 cut(s) 824
Asp700I GAANNNNTTC 1 cut(s) 701
AspLEI GCGC 1 cut(s) 782
AspS9I GGNCC 2 cut(s) 374, 612
AsuC2I CCSGG 1 cut(s) 711
AsuHPI GGTGA 1 cut(s) 133
AvaII GGWCC 2 cut(s) 374, 612
AxyI CCTNAGG 1 cut(s) 804
BalI TGGCCA 1 cut(s) 665
BanII GRGCYC 1 cut(s) 181
BbsI GAAGAC 2 cut(s) 179, 570
Bbv12I GWGCWC 1 cut(s) 181
BbvI GCAGC 1 cut(s) 311
BccI CCATC 4 cut(s) 21, 94, 420, 576
BciVI GTATCC 1 cut(s) 432
BcnI CCSGG 1 cut(s) 711
BcoDI GTCTC 2 cut(s) 132, 223
BcuI ACTAGT 1 cut(s) 845
BfaI CTAG 4 cut(s) 489, 506, 771, 846
BfuI GTATCC 1 cut(s) 432
BisI GCNGC 4 cut(s) 168, 300, 577, 768
BlsI GCNGC 4 cut(s) 169, 301, 578, 769
BmcAI AGTACT 2 cut(s) 276, 844
Bme1390I CCNGG 1 cut(s) 711
Bme18I GGWCC 2 cut(s) 374, 612
BmgT120I GGNCC 2 cut(s) 374, 612
BmrFI CCNGG 1 cut(s) 711
BmsI GCATC 2 cut(s) 382, 754
BpiI GAAGAC 2 cut(s) 179, 570
BpmI CTGGAG 1 cut(s) 439
BpuEI CTTGAG 1 cut(s) 165
BpuMI CCSGG 1 cut(s) 711
BsaBI GATNNNNATC 1 cut(s) 15
BsaHI GRCGYC 1 cut(s) 567
BsaJI CCNNGG 1 cut(s) 592
BsaWI WCCGGW 1 cut(s) 59
BsaXI ACNNNNNCTCC 4 cut(s) 105, 135, 437, 467
Bsc4I CCNNNNNNNGG 1 cut(s) 435
Bse1I ACTGG 1 cut(s) 282
Bse21I CCTNAGG 1 cut(s) 804
Bse3DI GCAATG 1 cut(s) 791
Bse8I GATNNNNATC 1 cut(s) 15
BseDI CCNNGG 1 cut(s) 592
BseGI GGATG 1 cut(s) 105
BseJI GATNNNNATC 1 cut(s) 15
BseLI CCNNNNNNNGG 1 cut(s) 435
BseMI GCAATG 1 cut(s) 791
BseMII CTCAG 3 cut(s) 187, 680, 795
BseNI ACTGG 1 cut(s) 282
BseRI GAGGAG 2 cut(s) 415, 821
BseXI GCAGC 1 cut(s) 311
Bsh1236I CGCG 1 cut(s) 50
Bsh1285I CGRYCG 1 cut(s) 48
BshFI GGCC 2 cut(s) 601, 665
BsiEI CGRYCG 1 cut(s) 48
BsiHKAI GWGCWC 1 cut(s) 181
BsiSI CCGG 2 cut(s) 60, 711
BslFI GGGAC 1 cut(s) 693
BslI CCNNNNNNNGG 1 cut(s) 435
BsmAI GTCTC 2 cut(s) 132, 223
BsmFI GGGAC 1 cut(s) 693
BsnI GGCC 2 cut(s) 601, 665
Bsp1286I GDGCHC 1 cut(s) 181
Bsp143I GATC 2 cut(s) 10, 223
Bsp68I TCGCGA 1 cut(s) 50
BspACI CCGC 5 cut(s) 5, 104, 167, 576, 768
BspANI GGCC 2 cut(s) 601, 665
BspCNI CTCAG 3 cut(s) 186, 681, 796
BspFNI CGCG 1 cut(s) 50
BspPI GGATC 2 cut(s) 18, 218
BspQI GCTCTTC 1 cut(s) 581
BsrBI CCGCTC 1 cut(s) 576
BsrDI GCAATG 1 cut(s) 791
BsrI ACTGG 1 cut(s) 282
BssECI CCNNGG 1 cut(s) 592
BssMI GATC 2 cut(s) 10, 223
BssNI GRCGYC 1 cut(s) 567
BssT1I CCWWGG 1 cut(s) 592
Bst4CI ACNGT 3 cut(s) 46, 121, 522
Bst6I CTCTTC 2 cut(s) 89, 581
BstACI GRCGYC 1 cut(s) 567
BstC8I GCNNGC 2 cut(s) 636, 789
BstDEI CTNAG 5 cut(s) 173, 378, 479, 689, 804
BstF5I GGATG 1 cut(s) 105
BstFNI CGCG 1 cut(s) 50
BstHHI GCGC 1 cut(s) 782
BstKTI GATC 2 cut(s) 13, 226
BstMAI GTCTC 2 cut(s) 132, 223
BstMBI GATC 2 cut(s) 10, 223
BstMCI CGRYCG 1 cut(s) 48
BstMWI GCNNNNNNNGC 5 cut(s) 167, 176, 764, 788, 793
BstNSI RCATGY 1 cut(s) 344
BstSCI CCNGG 1 cut(s) 709
BstUI CGCG 1 cut(s) 50
BstV1I GCAGC 1 cut(s) 311
BstV2I GAAGAC 2 cut(s) 179, 570
Bsu36I CCTNAGG 1 cut(s) 804
BsuI GTATCC 1 cut(s) 432
BsuRI GGCC 2 cut(s) 601, 665
BtsCI GGATG 1 cut(s) 105
BtsIMutI CAGTG 1 cut(s) 518
BtuMI TCGCGA 1 cut(s) 50
Cac8I GCNNGC 2 cut(s) 636, 789
CfoI GCGC 1 cut(s) 782
Cfr13I GGNCC 2 cut(s) 374, 612
Csp6I GTAC 2 cut(s) 275, 843
CviAII CATG 3 cut(s) 252, 341, 406
CviQI GTAC 2 cut(s) 275, 843
DdeI CTNAG 5 cut(s) 173, 378, 479, 689, 804
DpnI GATC 2 cut(s) 12, 225
DpnII GATC 2 cut(s) 10, 223
EaeI YGGCCR 1 cut(s) 663
Eam1104I CTCTTC 2 cut(s) 89, 581
EarI CTCTTC 2 cut(s) 89, 581
EciI GGCGGA 1 cut(s) 119
Ecl136II GAGCTC 1 cut(s) 179
Eco130I CCWWGG 1 cut(s) 592
Eco24I GRGCYC 1 cut(s) 181
Eco32I GATATC 2 cut(s) 319, 535
Eco47I GGWCC 2 cut(s) 374, 612
Eco53kI GAGCTC 1 cut(s) 179
Eco81I CCTNAGG 1 cut(s) 804
EcoICRI GAGCTC 1 cut(s) 179
EcoRV GATATC 2 cut(s) 319, 535
EcoT14I CCWWGG 1 cut(s) 592
EcoT38I GRGCYC 1 cut(s) 181
ErhI CCWWGG 1 cut(s) 592
FaeI CATG 3 cut(s) 255, 344, 409
FaqI GGGAC 1 cut(s) 693
FatI CATG 3 cut(s) 251, 340, 405
Fnu4HI GCNGC 4 cut(s) 168, 300, 577, 768
FokI GGATG 1 cut(s) 112
FriOI GRGCYC 1 cut(s) 181
Fsp4HI GCNGC 4 cut(s) 168, 300, 577, 768
FspBI CTAG 4 cut(s) 489, 506, 771, 846
GlaI GCGC 1 cut(s) 781
GluI GCNGC 4 cut(s) 168, 300, 577, 768
GsuI CTGGAG 1 cut(s) 439
HaeIII GGCC 2 cut(s) 601, 665
HapII CCGG 2 cut(s) 60, 711
HhaI GCGC 1 cut(s) 782
Hin1I GRCGYC 1 cut(s) 567
Hin1II CATG 3 cut(s) 255, 344, 409
Hin6I GCGC 1 cut(s) 780
HinP1I GCGC 1 cut(s) 780
HindIII AAGCTT 1 cut(s) 632
HinfI GANTC 3 cut(s) 459, 733, 811
HpaII CCGG 2 cut(s) 60, 711
HphI GGTGA 1 cut(s) 133
Hpy166II GTNNAC 1 cut(s) 292
Hpy188I TCNGA 7 cut(s) 21, 37, 176, 223, 438, 526, 690
Hpy188III TCNNGA 6 cut(s) 49, 456, 506, 560, 571, 803
Hpy8I GTNNAC 1 cut(s) 292
HpyAV CCTTC 2 cut(s) 211, 237
HpyCH4III ACNGT 3 cut(s) 46, 121, 522
HpyCH4IV ACGT 2 cut(s) 447, 567
HpyCH4V TGCA 5 cut(s) 86, 156, 272, 299, 638
HpyF10VI GCNNNNNNNGC 5 cut(s) 167, 176, 764, 788, 793
HpyF3I CTNAG 5 cut(s) 173, 378, 479, 689, 804
HpySE526I ACGT 2 cut(s) 447, 567
Hsp92I GRCGYC 1 cut(s) 567
Hsp92II CATG 3 cut(s) 255, 344, 409
HspAI GCGC 1 cut(s) 780
Kzo9I GATC 2 cut(s) 10, 223
LguI GCTCTTC 1 cut(s) 581
LmnI GCTCC 2 cut(s) 62, 458
LpnPI CCDG 7 cut(s) 73, 148, 263, 469, 724, 773, 816
Lsp1109I GCAGC 1 cut(s) 311
LweI GCATC 2 cut(s) 382, 754
MaeI CTAG 4 cut(s) 489, 506, 771, 846
MaeII ACGT 2 cut(s) 447, 567
MaeIII GTNAC 4 cut(s) 55, 115, 196, 381
MalI GATC 2 cut(s) 12, 225
MbiI CCGCTC 1 cut(s) 576
MboI GATC 2 cut(s) 10, 223
MboII GAAGA 6 cut(s) 36, 106, 179, 222, 575, 598
MhlI GDGCHC 1 cut(s) 181
MlsI TGGCCA 1 cut(s) 665
MluCI AATT 3 cut(s) 643, 824, 869
MluNI TGGCCA 1 cut(s) 665
MlyI GAGTC 2 cut(s) 453, 727
MmeI TCCRAC 4 cut(s) 15, 416, 504, 839
Mox20I TGGCCA 1 cut(s) 665
MroXI GAANNNNTTC 1 cut(s) 701
MscI TGGCCA 1 cut(s) 665
MseI TTAA 3 cut(s) 128, 312, 444
Msp20I TGGCCA 1 cut(s) 665
MspA1I CMGCKG 1 cut(s) 302
MspI CCGG 2 cut(s) 60, 711
MspR9I CCNGG 1 cut(s) 711
MvnI CGCG 1 cut(s) 50
MwoI GCNNNNNNNGC 5 cut(s) 167, 176, 764, 788, 793
NciI CCSGG 1 cut(s) 711
NdeII GATC 2 cut(s) 10, 223
NlaIII CATG 3 cut(s) 255, 344, 409
NmeAIII GCCGAG 2 cut(s) 370, 441
NmuCI GTSAC 2 cut(s) 55, 115
NruI TCGCGA 1 cut(s) 50
NspI RCATGY 1 cut(s) 344
PciSI GCTCTTC 1 cut(s) 581
PdmI GAANNNNTTC 1 cut(s) 701
PfeI GAWTC 1 cut(s) 811
PflFI GACNNNGTC 1 cut(s) 44
PkrI GCNGC 4 cut(s) 169, 301, 578, 769
PleI GAGTC 2 cut(s) 453, 727
PpsI GAGTC 2 cut(s) 453, 727
Psp124BI GAGCTC 1 cut(s) 181
Psp1406I AACGTT 1 cut(s) 447
PspPI GGNCC 2 cut(s) 374, 612
PsyI GACNNNGTC 1 cut(s) 44
PvuII CAGCTG 1 cut(s) 302
RruI TCGCGA 1 cut(s) 50
RsaI GTAC 2 cut(s) 276, 844
RsaNI GTAC 2 cut(s) 275, 843
SacI GAGCTC 1 cut(s) 181
SapI GCTCTTC 1 cut(s) 581
SaqAI TTAA 3 cut(s) 128, 312, 444
SatI GCNGC 4 cut(s) 168, 300, 577, 768
Sau3AI GATC 2 cut(s) 10, 223
Sau96I GGNCC 2 cut(s) 374, 612
ScaI AGTACT 2 cut(s) 276, 844
SchI GAGTC 2 cut(s) 453, 727
ScrFI CCNGG 1 cut(s) 711
SduI GDGCHC 1 cut(s) 181
SfaNI GCATC 2 cut(s) 382, 754
SinI GGWCC 2 cut(s) 374, 612
SmlI CTYRAG 1 cut(s) 180
SmoI CTYRAG 1 cut(s) 180
SpeI ACTAGT 1 cut(s) 845
Sse9I AATT 3 cut(s) 643, 824, 869
SsiI CCGC 5 cut(s) 5, 104, 167, 576, 768
SspMI CTAG 4 cut(s) 489, 506, 771, 846
SstI GAGCTC 1 cut(s) 181
StyD4I CCNGG 1 cut(s) 709
StyI CCWWGG 1 cut(s) 592
TaaI ACNGT 3 cut(s) 46, 121, 522
TaiI ACGT 2 cut(s) 450, 570
TaqI TCGA 2 cut(s) 13, 537
TaqII GACCGA 1 cut(s) 629
TasI AATT 3 cut(s) 643, 824, 869
TatI WGTACW 2 cut(s) 274, 842
TauI GCSGC 3 cut(s) 170, 579, 770
TfiI GAWTC 1 cut(s) 811
Tru1I TTAA 3 cut(s) 128, 312, 444
Tru9I TTAA 3 cut(s) 128, 312, 444
TscAI CASTG 1 cut(s) 525
TseFI GTSAC 2 cut(s) 55, 115
TseI GCWGC 1 cut(s) 299
Tsp45I GTSAC 2 cut(s) 55, 115
TspDTI ATGAA 5 cut(s) 179, 240, 339, 371, 636
TspGWI ACGGA 1 cut(s) 418
TspRI CASTG 1 cut(s) 525
Tth111I GACNNNGTC 1 cut(s) 44
VpaK11BI GGWCC 2 cut(s) 374, 612
XapI RAATTY 1 cut(s) 824
XbaI TCTAGA 1 cut(s) 505
XceI RCATGY 1 cut(s) 344
XmnI GAANNNNTTC 1 cut(s) 701
XspI CTAG 4 cut(s) 489, 506, 771, 846
ZraI GACGTC 1 cut(s) 568
ZrmI AGTACT 2 cut(s) 276, 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.