MD07G1062100.v1.1

Belongs to the short-chain dehydrogenases reductases (SDR) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008576
Physical Location & Seq
Reverse (-)
5842010 .. 5845557
3548 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1062100.v1.1.491

Sequence Viewer

Length: 930 bp
ATGGAGTCCAAAGAAGCTACCATCTCTTCTCCTTCTCCTTCTCCTTCTCGTTCCCTCTCCCCAGACCCAACCAGCAGATGGTGGTCGGAGGACACGGTTGCGATAGTGACGGGAGCAAACAAGGGAATAGGGTTTGCAGTTGCGAAGAGGATGGCGGAGTTGGGAGTGAGAGTGATATTAACGGCCAGAGACAAGGAAAGGGGTGGCAAGGCTGTTGAGGCACTCAGAGCTCAAGGTCTTCACAGTGTAGTCTTCTCTTGCCTTGATGTCTCCGATCCTGCTTCTATCAAATCTTTCACCTTATGGTTCCAAAAAACCTATGCAGTATTGGATATTCTTGTGAACAATGCAGCTGTGTCGTTTAACGATATCAATGAAAATTCTGTGGAACATGCTGAGGCAGTGATGAAAACCAACTTCTATGGACCTAAGTTACTGACGGAGGCCCTCTTGCCCATGTTCCGTCGTAGCACCTCCTCTGTTGGTCGGATACTTAACGTTAGCTCCAGACTCGGCTCACTAAATAAACTGAGAAACCCTAGCACAAAACAACTTCTAGAAAGTGAAAGCCTATCGGAAAAGGATATCGAGGGGGTGGTTAGTTTGTTTCTTGAAGATGTCAAGAGCGGGAGATGGAAGAGCCAAGGGTGGCCTGAAGTATGGACTGACTATTCTGTATCAAAGCTTGCACTCAATGCATACACAAGAGTTTTGGCCAAGCGATATGAGGGGAGGGGTTTGAGCGTGAATAGCTTCTGTCCCGGCTTCACGCAGACATCTATGACTCGCTGTAAGGGCGACCACACAGCCGATGATGCCGCTAGTGTTGGGGCAAGGCTGGCACTGCTTCCTCCTAAGGAGATTCCAACAGGGAAATTTTTTTGGTGGGTTAGTACTAGTAGTAGTAATGTCATACTTTCTAAATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00590 ko00790 ko00980 ko01100 ko05204 map00590 map00790 map00980 map01100 map05204
Pfam Domains
Protein Families
adh_short

Protein Analysis

310

Amino Acids

33.83

Weight (kDa)

9.28

Isoelectric Point (pI)

46.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 33 - 173 1.1e-29 short chain dehydrogenase
KR PF08659 34 - 152 6.5e-08 KR domain
adh_short_C2 PF13561 38 - 170 6.5e-22 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 215 - 264 1.2e-06 Enoyl-(Acyl carrier protein) reductase
adh_short PF00106 220 - 265 1.8e-07 short chain dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 78
AccBSI CCGCTC 1 cut(s) 627
AciI CCGC 3 cut(s) 155, 627, 819
AclI AACGTT 1 cut(s) 498
AclWI GGATC 1 cut(s) 269
AcoI YGGCCR 2 cut(s) 183, 714
AcsI RAATTY 2 cut(s) 379, 875
AcuI CTGAAG 1 cut(s) 675
AfaI GTAC 1 cut(s) 895
AfiI CCNNNNNNNGG 1 cut(s) 78
AgsI TTSAA 1 cut(s) 614
AhlI ACTAGT 1 cut(s) 896
AluBI AGCT 6 cut(s) 17, 230, 353, 504, 685, 753
AluI AGCT 6 cut(s) 17, 230, 353, 504, 685, 753
Alw21I GWGCWC 1 cut(s) 232
Alw26I GTCTC 2 cut(s) 183, 274
AlwI GGATC 1 cut(s) 269
AoxI GGCC 4 cut(s) 183, 444, 650, 714
ApeKI GCWGC 1 cut(s) 350
ApoI RAATTY 2 cut(s) 379, 875
Asp700I GAANNNNTTC 1 cut(s) 752
AspS9I GGNCC 2 cut(s) 425, 445
AsuC2I CCSGG 1 cut(s) 762
AsuHPI GGTGA 1 cut(s) 289
AvaII GGWCC 1 cut(s) 425
AxyI CCTNAGG 1 cut(s) 855
BalI TGGCCA 1 cut(s) 716
BanII GRGCYC 1 cut(s) 232
BbsI GAAGAC 2 cut(s) 230, 244
Bbv12I GWGCWC 1 cut(s) 232
BbvCI CCTCAGC 1 cut(s) 396
BbvI GCAGC 1 cut(s) 362
BccI CCATC 4 cut(s) 29, 72, 145, 627
BceAI ACGGC 1 cut(s) 198
BcgI CGANNNNNNTGC 2 cut(s) 339, 373
BciVI GTATCC 1 cut(s) 483
BcnI CCSGG 1 cut(s) 762
BcoDI GTCTC 2 cut(s) 183, 274
BcuI ACTAGT 1 cut(s) 896
BfaI CTAG 4 cut(s) 540, 557, 822, 897
BfuI GTATCC 1 cut(s) 483
BisI GCNGC 2 cut(s) 351, 819
BlsI GCNGC 2 cut(s) 352, 820
BmcAI AGTACT 1 cut(s) 895
Bme1390I CCNGG 1 cut(s) 762
Bme18I GGWCC 1 cut(s) 425
BmgT120I GGNCC 2 cut(s) 425, 445
BmiI GGNNCC 1 cut(s) 308
BmrFI CCNGG 1 cut(s) 762
BmsI GCATC 1 cut(s) 805
BpiI GAAGAC 2 cut(s) 230, 244
BpmI CTGGAG 1 cut(s) 490
Bpu10I CCTNAGC 1 cut(s) 396
BpuEI CTTGAG 1 cut(s) 216
BpuMI CCSGG 1 cut(s) 762
BsaJI CCNNGG 1 cut(s) 643
BsaXI ACNNNNNCTCC 4 cut(s) 156, 186, 488, 518
Bsc4I CCNNNNNNNGG 1 cut(s) 78
Bse21I CCTNAGG 1 cut(s) 855
BseDI CCNNGG 1 cut(s) 643
BseGI GGATG 1 cut(s) 156
BseLI CCNNNNNNNGG 1 cut(s) 78
BseMII CTCAG 3 cut(s) 238, 387, 521
BseRI GAGGAG 1 cut(s) 466
BseXI GCAGC 1 cut(s) 362
BshFI GGCC 4 cut(s) 185, 446, 652, 716
BsiHKAI GWGCWC 1 cut(s) 232
BsiSI CCGG 1 cut(s) 762
BslFI GGGAC 1 cut(s) 744
BslI CCNNNNNNNGG 1 cut(s) 78
BsmAI GTCTC 2 cut(s) 183, 274
BsmFI GGGAC 1 cut(s) 744
BsnI GGCC 4 cut(s) 185, 446, 652, 716
Bsp1286I GDGCHC 1 cut(s) 232
Bsp143I GATC 1 cut(s) 274
BspACI CCGC 3 cut(s) 155, 627, 819
BspANI GGCC 4 cut(s) 185, 446, 652, 716
BspCNI CTCAG 3 cut(s) 237, 388, 522
BspLI GGNNCC 1 cut(s) 308
BspPI GGATC 1 cut(s) 269
BspQI GCTCTTC 1 cut(s) 632
BsrBI CCGCTC 1 cut(s) 627
BssECI CCNNGG 1 cut(s) 643
BssMI GATC 1 cut(s) 274
BssT1I CCWWGG 1 cut(s) 643
Bst4CI ACNGT 2 cut(s) 97, 245
Bst6I CTCTTC 3 cut(s) 31, 140, 632
BstAPI GCANNNNNTGC 1 cut(s) 695
BstC8I GCNNGC 2 cut(s) 687, 840
BstDEI CTNAG 5 cut(s) 224, 396, 429, 530, 855
BstF5I GGATG 1 cut(s) 156
BstKTI GATC 1 cut(s) 277
BstMAI GTCTC 2 cut(s) 183, 274
BstMBI GATC 1 cut(s) 274
BstMWI GCNNNNNNNGC 8 cut(s) 218, 227, 695, 750, 795, 815, 839, 844
BstNSI RCATGY 1 cut(s) 395
BstSCI CCNGG 1 cut(s) 760
BstV1I GCAGC 1 cut(s) 362
BstV2I GAAGAC 2 cut(s) 230, 244
Bsu36I CCTNAGG 1 cut(s) 855
BsuI GTATCC 1 cut(s) 483
BsuRI GGCC 4 cut(s) 185, 446, 652, 716
BtsCI GGATG 1 cut(s) 156
BtsI GCAGTG 2 cut(s) 408, 842
BtsIMutI CAGTG 3 cut(s) 250, 408, 842
Cac8I GCNNGC 2 cut(s) 687, 840
Cfr13I GGNCC 2 cut(s) 425, 445
Csp6I GTAC 1 cut(s) 894
CviAII CATG 2 cut(s) 392, 457
CviQI GTAC 1 cut(s) 894
DdeI CTNAG 5 cut(s) 224, 396, 429, 530, 855
DpnI GATC 1 cut(s) 276
DpnII GATC 1 cut(s) 274
EaeI YGGCCR 2 cut(s) 183, 714
Eam1104I CTCTTC 3 cut(s) 31, 140, 632
EarI CTCTTC 3 cut(s) 31, 140, 632
EciI GGCGGA 1 cut(s) 170
Ecl136II GAGCTC 1 cut(s) 230
Eco130I CCWWGG 1 cut(s) 643
Eco24I GRGCYC 1 cut(s) 232
Eco32I GATATC 2 cut(s) 370, 586
Eco47I GGWCC 1 cut(s) 425
Eco53kI GAGCTC 1 cut(s) 230
Eco57I CTGAAG 1 cut(s) 675
Eco81I CCTNAGG 1 cut(s) 855
EcoICRI GAGCTC 1 cut(s) 230
EcoO109I RGGNCCY 1 cut(s) 445
EcoRV GATATC 2 cut(s) 370, 586
EcoT14I CCWWGG 1 cut(s) 643
EcoT22I ATGCAT 1 cut(s) 700
EcoT38I GRGCYC 1 cut(s) 232
ErhI CCWWGG 1 cut(s) 643
FaeI CATG 2 cut(s) 395, 460
FaqI GGGAC 1 cut(s) 744
FatI CATG 2 cut(s) 391, 456
FauI CCCGC 1 cut(s) 620
Fnu4HI GCNGC 2 cut(s) 351, 819
FokI GGATG 1 cut(s) 163
FriOI GRGCYC 1 cut(s) 232
Fsp4HI GCNGC 2 cut(s) 351, 819
FspBI CTAG 4 cut(s) 540, 557, 822, 897
GluI GCNGC 2 cut(s) 351, 819
GsuI CTGGAG 1 cut(s) 490
HaeIII GGCC 4 cut(s) 185, 446, 652, 716
HapII CCGG 1 cut(s) 762
Hin1II CATG 2 cut(s) 395, 460
HindIII AAGCTT 1 cut(s) 683
HinfI GANTC 4 cut(s) 5, 510, 784, 862
HpaII CCGG 1 cut(s) 762
HphI GGTGA 1 cut(s) 289
Hpy166II GTNNAC 1 cut(s) 343
Hpy188I TCNGA 5 cut(s) 88, 227, 274, 489, 577
Hpy188III TCNNGA 4 cut(s) 507, 557, 611, 622
Hpy8I GTNNAC 1 cut(s) 343
Hpy99I CGWCG 1 cut(s) 468
HpyAV CCTTC 3 cut(s) 42, 48, 54
HpyCH4III ACNGT 2 cut(s) 97, 245
HpyCH4IV ACGT 1 cut(s) 498
HpyCH4V TGCA 5 cut(s) 137, 323, 350, 689, 698
HpyF10VI GCNNNNNNNGC 8 cut(s) 218, 227, 695, 750, 795, 815, 839, 844
HpyF3I CTNAG 5 cut(s) 224, 396, 429, 530, 855
HpySE526I ACGT 1 cut(s) 498
Hsp92II CATG 2 cut(s) 395, 460
Kzo9I GATC 1 cut(s) 274
LguI GCTCTTC 1 cut(s) 632
LmnI GCTCC 2 cut(s) 113, 509
LpnPI CCDG 9 cut(s) 75, 85, 199, 291, 520, 666, 775, 824, 855
Lsp1109I GCAGC 1 cut(s) 362
LweI GCATC 1 cut(s) 805
MaeI CTAG 4 cut(s) 540, 557, 822, 897
MaeII ACGT 1 cut(s) 498
MaeIII GTNAC 2 cut(s) 106, 432
MalI GATC 1 cut(s) 276
MbiI CCGCTC 1 cut(s) 627
MboI GATC 1 cut(s) 274
MboII GAAGA 6 cut(s) 18, 157, 230, 244, 626, 649
MhlI GDGCHC 1 cut(s) 232
MlsI TGGCCA 1 cut(s) 716
MluCI AATT 3 cut(s) 379, 875, 923
MluNI TGGCCA 1 cut(s) 716
MlyI GAGTC 3 cut(s) 14, 504, 778
MmeI TCCRAC 3 cut(s) 66, 467, 890
Mox20I TGGCCA 1 cut(s) 716
Mph1103I ATGCAT 1 cut(s) 700
MroXI GAANNNNTTC 1 cut(s) 752
MscI TGGCCA 1 cut(s) 716
MseI TTAA 3 cut(s) 179, 363, 495
Msp20I TGGCCA 1 cut(s) 716
MspA1I CMGCKG 1 cut(s) 353
MspI CCGG 1 cut(s) 762
MspR9I CCNGG 1 cut(s) 762
MwoI GCNNNNNNNGC 8 cut(s) 218, 227, 695, 750, 795, 815, 839, 844
NciI CCSGG 1 cut(s) 762
NdeII GATC 1 cut(s) 274
NlaIII CATG 2 cut(s) 395, 460
NlaIV GGNNCC 1 cut(s) 308
NmeAIII GCCGAG 1 cut(s) 492
NmuCI GTSAC 1 cut(s) 106
NsiI ATGCAT 1 cut(s) 700
NspI RCATGY 1 cut(s) 395
PciSI GCTCTTC 1 cut(s) 632
PdmI GAANNNNTTC 1 cut(s) 752
PfeI GAWTC 1 cut(s) 862
PflMI CCANNNNNTGG 1 cut(s) 78
PkrI GCNGC 2 cut(s) 352, 820
PleI GAGTC 3 cut(s) 13, 504, 778
PpsI GAGTC 3 cut(s) 13, 504, 778
Psp124BI GAGCTC 1 cut(s) 232
Psp1406I AACGTT 1 cut(s) 498
PspN4I GGNNCC 1 cut(s) 308
PspPI GGNCC 2 cut(s) 425, 445
PvuII CAGCTG 1 cut(s) 353
RsaI GTAC 1 cut(s) 895
RsaNI GTAC 1 cut(s) 894
SacI GAGCTC 1 cut(s) 232
SapI GCTCTTC 1 cut(s) 632
SaqAI TTAA 3 cut(s) 179, 363, 495
SatI GCNGC 2 cut(s) 351, 819
Sau3AI GATC 1 cut(s) 274
Sau96I GGNCC 2 cut(s) 425, 445
ScaI AGTACT 1 cut(s) 895
SchI GAGTC 3 cut(s) 14, 504, 778
ScrFI CCNGG 1 cut(s) 762
SduI GDGCHC 1 cut(s) 232
SfaNI GCATC 1 cut(s) 805
SinI GGWCC 1 cut(s) 425
SmlI CTYRAG 1 cut(s) 231
SmoI CTYRAG 1 cut(s) 231
SpeI ACTAGT 1 cut(s) 896
Sse9I AATT 3 cut(s) 379, 875, 923
SsiI CCGC 3 cut(s) 155, 627, 819
SspMI CTAG 4 cut(s) 540, 557, 822, 897
SstI GAGCTC 1 cut(s) 232
StyD4I CCNGG 1 cut(s) 760
StyI CCWWGG 1 cut(s) 643
TaaI ACNGT 2 cut(s) 97, 245
TaiI ACGT 1 cut(s) 501
TaqI TCGA 1 cut(s) 588
TasI AATT 3 cut(s) 379, 875, 923
TatI WGTACW 1 cut(s) 893
TauI GCSGC 1 cut(s) 821
TfiI GAWTC 1 cut(s) 862
Tru1I TTAA 3 cut(s) 179, 363, 495
Tru9I TTAA 3 cut(s) 179, 363, 495
TscAI CASTG 3 cut(s) 250, 408, 849
TseFI GTSAC 1 cut(s) 106
TseI GCWGC 1 cut(s) 350
Tsp45I GTSAC 1 cut(s) 106
TspDTI ATGAA 2 cut(s) 390, 422
TspGWI ACGGA 2 cut(s) 452, 455
TspRI CASTG 3 cut(s) 250, 408, 849
Van91I CCANNNNNTGG 1 cut(s) 78
VpaK11BI GGWCC 1 cut(s) 425
XapI RAATTY 2 cut(s) 379, 875
XbaI TCTAGA 1 cut(s) 556
XceI RCATGY 1 cut(s) 395
XcmI CCANNNNNNNNNTGG 1 cut(s) 75
XmnI GAANNNNTTC 1 cut(s) 752
XspI CTAG 4 cut(s) 540, 557, 822, 897
ZrmI AGTACT 1 cut(s) 895
Zsp2I ATGCAT 1 cut(s) 700
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.