MD02G1260600.v1.1

Sucrose-6-phosphate phosphohydrolase C-terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005986
Physical Location & Seq
Reverse (-)
31412528 .. 31415706
3179 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1260600.v1.1.491

Sequence Viewer

Length: 1377 bp
ATGATGAACATGAATTGTCGTCTGAATGGCCCTGCTCGTCTGATGCTAGTCTCAGATCTTGACTACACCTTGGTTGATCATGATGCGCCTGATAATCTCTCTCTTCTCAAGTTCAATGCGCTGTGGGAATCATATTACCGCCATGATTCTCTGCTGGTATTTTCCACTGGAAGATCACCCATCACGTACAAACCTCTGAGGAATCAGAAACCCTTGCTAACCCCCGACATCACCATCATGTCCGTCGGCACTGAGATTATGTATGGCGGTGGTACCGATATGATTCCTGATCTTGGTTGGCAACAGTTTCTCAGTCACAGATGGGATAGAGCCATAGTTGTTGAAGAAACTAATCAGTTTCCTCAACTTACTCCTCAGGCAGAGGGAGAGCAACGACCTCACAAGGTTAGCTTTTATGTTGACAAGGTTATGGCATTCGAAATAATGAAAGTTTTATCACAACGGTTGGAGAAACGCGGGTTAAGATTTCCTCGATTGTTTTCGTTCTATGAACCATTTTGCTACACTCCAGAGCATTCACAACTTCAATTGAAAACAAAACTGTTAGACGTAAAGATCATTTATAGCAGTGGGATTGCTTTGGACGTTCTACCAAAAGGTGCTGGTAAAGGAAAAGCTCTTGCATATCTGTTAAAGAAATTTAAGTTTGAGGAGAAACTTCCACACAACACTCTTGTTTGTGGCGACTCTGGAAACGATGCTGAACTCTTCAGTCTCCCTCAAGTGTATGGGGTGATGGTGAGTAATGCACAAGAAGAATTGTTGCAGTGGTATGAAGAAAATGCAAGCGACAATCCTAATATGCTTCATGCAACTGAGAGATGTGCAGCTGGTATAATACAAGCGCTTGGACATTTTCATCTAGGTCCAAATGTATCGCCAAGAGATTTCAAAGACTTCCGAAAGTGCAAAGTGGAAATGTCCAGTCCTGCTCATGAGGTAGTCAAATTCTTTTTGTTCTATGAGAGATGGCGCCGTGCTGAAGTAGAGAAATCTGAGGAGTATATCCAAAACTTAAAACCAGTCTTTCATTCATCAGGTATCTTTGTCCATCCCTCCGGGGTTGAGCTACCTATCCACCAGTGCATAGATTCGTTGGCAGGGTTGCATGGAGATAAGCAGGGAAAACAATTTTGGACATGGGTGGATCGGTTATCGTTGGTTCAAATTGGCTCAAATGCATGGCTCGTGAAGTTTAATAAGTGGGAATTGTGTGAGAACGAGCGGCAATGCTGTCTGACTACAGTCCTGATGAGTTCGAAGGCTGAGGTGCCAGATGCTTTCACTTGGCTGCACATGCATCAGACATGGCTGGACGGTTTGGAAATTAAAGAACAAGAAAGATGGTTATTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 map00500
Pfam Domains
Protein Families
S6PP S6PP_C

Protein Analysis

459

Amino Acids

52.86

Weight (kDa)

6.26

Isoelectric Point (pI)

40.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S6PP PF05116 13 - 169 4.1e-48 Sucrose-6F-phosphate phosphohydrolase
S6PP PF05116 183 - 294 5.3e-42 Sucrose-6F-phosphate phosphohydrolase
Hydrolase_3 PF08282 187 - 263 1e-08 haloacid dehalogenase-like hydrolase
S6PP_C PF08472 317 - 445 1.3e-59 Sucrose-6-phosphate phosphohydrolase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 272
AccB1I GGYRCC 3 cut(s) 272, 993, 1291
AccBSI CCGCTC 1 cut(s) 1246
AccII CGCG 1 cut(s) 477
AciI CCGC 4 cut(s) 139, 267, 477, 1246
AclWI GGATC 1 cut(s) 1176
AcsI RAATTY 2 cut(s) 659, 968
AcuI CTGAAG 2 cut(s) 715, 1023
AcyI GRCGYC 1 cut(s) 994
AfaI GTAC 2 cut(s) 188, 274
AfeI AGCGCT 1 cut(s) 867
AfiI CCNNNNNNNGG 1 cut(s) 293
AgsI TTSAA 6 cut(s) 115, 344, 548, 553, 913, 1187
AjuI GAANNNNNNNTTGG 2 cut(s) 1138, 1170
AluBI AGCT 4 cut(s) 411, 638, 851, 1090
AluI AGCT 4 cut(s) 411, 638, 851, 1090
Alw26I GTCTC 2 cut(s) 55, 740
AlwI GGATC 1 cut(s) 1176
Aor51HI AGCGCT 1 cut(s) 867
AoxI GGCC 1 cut(s) 28
ApeKI GCWGC 2 cut(s) 848, 1312
ApoI RAATTY 2 cut(s) 659, 968
Asp718I GGTACC 1 cut(s) 272
AspLEI GCGC 4 cut(s) 88, 121, 868, 996
AspS9I GGNCC 2 cut(s) 29, 887
AsuC2I CCSGG 1 cut(s) 1081
AsuHPI GGTGA 4 cut(s) 168, 223, 766, 772
AsuII TTCGAA 2 cut(s) 438, 1280
AvaII GGWCC 1 cut(s) 887
AxyI CCTNAGG 1 cut(s) 375
BanI GGYRCC 3 cut(s) 272, 993, 1291
BauI CACGAG 1 cut(s) 1208
BbvCI CCTCAGC 1 cut(s) 1287
BbvI GCAGC 2 cut(s) 860, 1299
BccI CCATC 7 cut(s) 188, 242, 315, 751, 984, 1080, 1359
BceAI ACGGC 1 cut(s) 981
BclI TGATCA 1 cut(s) 76
BcnI CCSGG 1 cut(s) 1081
BcoDI GTCTC 2 cut(s) 55, 740
BfaI CTAG 2 cut(s) 47, 884
BfmI CTRYAG 1 cut(s) 1263
BfoI RGCGCY 2 cut(s) 869, 997
BglII AGATCT 1 cut(s) 55
BisI GCNGC 3 cut(s) 849, 1247, 1313
BlsI GCNGC 3 cut(s) 850, 1248, 1314
Bme1390I CCNGG 1 cut(s) 1081
Bme18I GGWCC 1 cut(s) 887
BmgT120I GGNCC 2 cut(s) 29, 887
BmiI GGNNCC 3 cut(s) 274, 995, 1293
BmrFI CCNGG 1 cut(s) 1081
BmsI GCATC 5 cut(s) 33, 73, 709, 1288, 1330
BoxI GACNNNNGTC 1 cut(s) 1265
BpmI CTGGAG 1 cut(s) 513
Bpu10I CCTNAGC 1 cut(s) 1287
Bpu14I TTCGAA 2 cut(s) 438, 1280
BpuEI CTTGAG 2 cut(s) 92, 726
BpuMI CCSGG 1 cut(s) 1081
BsaAI YACGTR 1 cut(s) 186
BsaHI GRCGYC 1 cut(s) 994
BsaJI CCNNGG 2 cut(s) 69, 1080
Bsc4I CCNNNNNNNGG 1 cut(s) 293
Bse1I ACTGG 4 cut(s) 172, 945, 1043, 1102
Bse21I CCTNAGG 1 cut(s) 375
Bse3DI GCAATG 1 cut(s) 1256
BseDI CCNNGG 2 cut(s) 69, 1080
BseGI GGATG 1 cut(s) 1072
BseLI CCNNNNNNNGG 1 cut(s) 293
BseMI GCAATG 1 cut(s) 1256
BseMII CTCAG 8 cut(s) 66, 188, 243, 325, 389, 828, 1008, 1278
BseNI ACTGG 4 cut(s) 172, 945, 1043, 1102
BseRI GAGGAG 3 cut(s) 363, 686, 1034
BseXI GCAGC 2 cut(s) 860, 1299
BsgI GTGCAG 2 cut(s) 867, 1298
Bsh1236I CGCG 1 cut(s) 477
BshFI GGCC 1 cut(s) 30
BshNI GGYRCC 3 cut(s) 272, 993, 1291
BsiSI CCGG 1 cut(s) 1080
BslI CCNNNNNNNGG 1 cut(s) 293
BsmAI GTCTC 2 cut(s) 55, 740
BsmI GAATGC 2 cut(s) 434, 535
BsnI GGCC 1 cut(s) 30
Bsp119I TTCGAA 2 cut(s) 438, 1280
Bsp143I GATC 6 cut(s) 55, 76, 173, 289, 576, 1168
BspACI CCGC 4 cut(s) 139, 267, 477, 1246
BspANI GGCC 1 cut(s) 30
BspCNI CTCAG 8 cut(s) 65, 189, 244, 324, 388, 829, 1009, 1279
BspFNI CGCG 1 cut(s) 477
BspHI TCATGA 2 cut(s) 79, 955
BspLI GGNNCC 3 cut(s) 274, 995, 1293
BspPI GGATC 1 cut(s) 1176
BspT104I TTCGAA 2 cut(s) 438, 1280
BspT107I GGYRCC 3 cut(s) 272, 993, 1291
BsrBI CCGCTC 1 cut(s) 1246
BsrDI GCAATG 1 cut(s) 1256
BsrI ACTGG 4 cut(s) 172, 945, 1043, 1102
BssECI CCNNGG 2 cut(s) 69, 1080
BssMI GATC 6 cut(s) 55, 76, 173, 289, 576, 1168
BssNI GRCGYC 1 cut(s) 994
BssSI CACGAG 1 cut(s) 1208
BssT1I CCWWGG 1 cut(s) 69
Bst2BI CACGAG 1 cut(s) 1208
Bst4CI ACNGT 5 cut(s) 306, 465, 564, 1267, 1340
Bst6I CTCTTC 2 cut(s) 108, 734
BstACI GRCGYC 1 cut(s) 994
BstBAI YACGTR 1 cut(s) 186
BstBI TTCGAA 2 cut(s) 438, 1280
BstC8I GCNNGC 1 cut(s) 808
BstDEI CTNAG 8 cut(s) 52, 197, 252, 311, 375, 837, 1017, 1287
BstF5I GGATG 1 cut(s) 1072
BstFNI CGCG 1 cut(s) 477
BstH2I RGCGCY 2 cut(s) 869, 997
BstHHI GCGC 4 cut(s) 88, 121, 868, 996
BstKTI GATC 6 cut(s) 58, 79, 176, 292, 579, 1171
BstMAI GTCTC 2 cut(s) 55, 740
BstMBI GATC 6 cut(s) 55, 76, 173, 289, 576, 1168
BstMWI GCNNNNNNNGC 1 cut(s) 1318
BstNSI RCATGY 1 cut(s) 1321
BstPAI GACNNNNGTC 1 cut(s) 1265
BstSCI CCNGG 1 cut(s) 1079
BstSFI CTRYAG 1 cut(s) 1263
BstUI CGCG 1 cut(s) 477
BstV1I GCAGC 2 cut(s) 860, 1299
BstX2I RGATCY 1 cut(s) 55
BstYI RGATCY 1 cut(s) 55
Bsu36I CCTNAGG 1 cut(s) 375
BsuRI GGCC 1 cut(s) 30
BtsCI GGATG 1 cut(s) 1072
BtsI GCAGTG 2 cut(s) 595, 794
BtsIMutI CAGTG 5 cut(s) 165, 249, 595, 794, 1109
Cac8I GCNNGC 1 cut(s) 808
CciI TCATGA 2 cut(s) 79, 955
CfoI GCGC 4 cut(s) 88, 121, 868, 996
Cfr13I GGNCC 2 cut(s) 29, 887
Csp6I GTAC 2 cut(s) 187, 273
CviQI GTAC 2 cut(s) 187, 273
DdeI CTNAG 8 cut(s) 52, 197, 252, 311, 375, 837, 1017, 1287
DinI GGCGCC 1 cut(s) 995
DpnI GATC 6 cut(s) 57, 78, 175, 291, 578, 1170
DpnII GATC 6 cut(s) 55, 76, 173, 289, 576, 1168
Eam1104I CTCTTC 2 cut(s) 108, 734
EarI CTCTTC 2 cut(s) 108, 734
Eco130I CCWWGG 1 cut(s) 69
Eco47I GGWCC 1 cut(s) 887
Eco47III AGCGCT 1 cut(s) 867
Eco57I CTGAAG 2 cut(s) 715, 1023
Eco81I CCTNAGG 1 cut(s) 375
EcoT14I CCWWGG 1 cut(s) 69
EcoT22I ATGCAT 2 cut(s) 1204, 1323
EgeI GGCGCC 1 cut(s) 995
EheI GGCGCC 1 cut(s) 995
ErhI CCWWGG 1 cut(s) 69
FalI AAGNNNNNCTT 2 cut(s) 395, 427
FauI CCCGC 1 cut(s) 470
FbaI TGATCA 1 cut(s) 76
Fnu4HI GCNGC 3 cut(s) 849, 1247, 1313
FokI GGATG 1 cut(s) 1059
Fsp4HI GCNGC 3 cut(s) 849, 1247, 1313
FspBI CTAG 2 cut(s) 47, 884
GlaI GCGC 4 cut(s) 87, 120, 867, 995
GluI GCNGC 3 cut(s) 849, 1247, 1313
GsuI CTGGAG 1 cut(s) 513
HaeII RGCGCY 2 cut(s) 869, 997
HaeIII GGCC 1 cut(s) 30
HapII CCGG 1 cut(s) 1080
HhaI GCGC 4 cut(s) 88, 121, 868, 996
Hin1I GRCGYC 1 cut(s) 994
Hin6I GCGC 4 cut(s) 86, 119, 866, 994
HinP1I GCGC 4 cut(s) 86, 119, 866, 994
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HinfI GANTC 6 cut(s) 128, 146, 202, 283, 707, 1112
HpaII CCGG 1 cut(s) 1080
HphI GGTGA 4 cut(s) 168, 223, 766, 772
Hpy166II GTNNAC 1 cut(s) 421
Hpy188I TCNGA 9 cut(s) 24, 42, 55, 198, 207, 923, 1018, 1260, 1326
Hpy188III TCNNGA 8 cut(s) 59, 80, 287, 530, 711, 956, 1210, 1270
Hpy8I GTNNAC 1 cut(s) 421
Hpy99I CGWCG 1 cut(s) 248
HpyAV CCTTC 1 cut(s) 1276
HpyCH4III ACNGT 5 cut(s) 306, 465, 564, 1267, 1340
HpyCH4IV ACGT 3 cut(s) 185, 570, 606
HpyF10VI GCNNNNNNNGC 1 cut(s) 1318
HpyF3I CTNAG 8 cut(s) 52, 197, 252, 311, 375, 837, 1017, 1287
HpySE526I ACGT 3 cut(s) 185, 570, 606
Hsp92I GRCGYC 1 cut(s) 994
HspAI GCGC 4 cut(s) 86, 119, 866, 994
KasI GGCGCC 1 cut(s) 993
KpnI GGTACC 1 cut(s) 276
Ksp22I TGATCA 1 cut(s) 76
Kzo9I GATC 6 cut(s) 55, 76, 173, 289, 576, 1168
Lsp1109I GCAGC 2 cut(s) 860, 1299
LweI GCATC 5 cut(s) 33, 73, 709, 1288, 1330
MaeI CTAG 2 cut(s) 47, 884
MaeII ACGT 3 cut(s) 185, 570, 606
MaeIII GTNAC 1 cut(s) 314
MalI GATC 6 cut(s) 57, 78, 175, 291, 578, 1170
MbiI CCGCTC 1 cut(s) 1246
MboI GATC 6 cut(s) 55, 76, 173, 289, 576, 1168
MboII GAAGA 6 cut(s) 95, 183, 356, 721, 788, 809
MfeI CAATTG 1 cut(s) 548
MflI RGATCY 1 cut(s) 55
MluCI AATT 9 cut(s) 13, 548, 659, 779, 968, 1151, 1188, 1229, 1347
Mly113I GGCGCC 1 cut(s) 994
MlyI GAGTC 1 cut(s) 701
MmeI TCCRAC 1 cut(s) 447
Mph1103I ATGCAT 2 cut(s) 1204, 1323
MseI TTAA 6 cut(s) 482, 653, 663, 1037, 1218, 1350
MslI CAYNNNNRTG 1 cut(s) 236
MspA1I CMGCKG 1 cut(s) 851
MspI CCGG 1 cut(s) 1080
MspR9I CCNGG 1 cut(s) 1081
MunI CAATTG 1 cut(s) 548
Mva1269I GAATGC 2 cut(s) 434, 535
MvnI CGCG 1 cut(s) 477
MwoI GCNNNNNNNGC 1 cut(s) 1318
NarI GGCGCC 1 cut(s) 994
NciI CCSGG 1 cut(s) 1081
NdeII GATC 6 cut(s) 55, 76, 173, 289, 576, 1168
NlaIV GGNNCC 3 cut(s) 274, 995, 1293
NmuCI GTSAC 1 cut(s) 314
NsiI ATGCAT 2 cut(s) 1204, 1323
NspI RCATGY 1 cut(s) 1321
NspV TTCGAA 2 cut(s) 438, 1280
PagI TCATGA 2 cut(s) 79, 955
PctI GAATGC 2 cut(s) 434, 535
PfeI GAWTC 5 cut(s) 128, 146, 202, 283, 1112
PkrI GCNGC 3 cut(s) 850, 1248, 1314
PleI GAGTC 1 cut(s) 701
PluTI GGCGCC 1 cut(s) 997
PpsI GAGTC 1 cut(s) 701
Ppu21I YACGTR 1 cut(s) 186
PshAI GACNNNNGTC 1 cut(s) 1265
PspN4I GGNNCC 3 cut(s) 274, 995, 1293
PspPI GGNCC 2 cut(s) 29, 887
PsuI RGATCY 1 cut(s) 55
PvuII CAGCTG 1 cut(s) 851
RsaI GTAC 2 cut(s) 188, 274
RsaNI GTAC 2 cut(s) 187, 273
RseI CAYNNNNRTG 1 cut(s) 236
SaqAI TTAA 6 cut(s) 482, 653, 663, 1037, 1218, 1350
SatI GCNGC 3 cut(s) 849, 1247, 1313
Sau3AI GATC 6 cut(s) 55, 76, 173, 289, 576, 1168
Sau96I GGNCC 2 cut(s) 29, 887
SchI GAGTC 1 cut(s) 701
ScrFI CCNGG 1 cut(s) 1081
SfaNI GCATC 5 cut(s) 33, 73, 709, 1288, 1330
SfcI CTRYAG 1 cut(s) 1263
SfoI GGCGCC 1 cut(s) 995
SfuI TTCGAA 2 cut(s) 438, 1280
SinI GGWCC 1 cut(s) 887
SmiMI CAYNNNNRTG 1 cut(s) 236
SmlI CTYRAG 2 cut(s) 107, 741
SmoI CTYRAG 2 cut(s) 107, 741
Sse9I AATT 9 cut(s) 13, 548, 659, 779, 968, 1151, 1188, 1229, 1347
SsiI CCGC 4 cut(s) 139, 267, 477, 1246
SspDI GGCGCC 1 cut(s) 993
SspMI CTAG 2 cut(s) 47, 884
StyD4I CCNGG 1 cut(s) 1079
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 5 cut(s) 306, 465, 564, 1267, 1340
TaiI ACGT 3 cut(s) 188, 573, 609
TaqI TCGA 3 cut(s) 438, 493, 1280
TasI AATT 9 cut(s) 13, 548, 659, 779, 968, 1151, 1188, 1229, 1347
TauI GCSGC 1 cut(s) 1249
TfiI GAWTC 5 cut(s) 128, 146, 202, 283, 1112
Tru1I TTAA 6 cut(s) 482, 653, 663, 1037, 1218, 1350
Tru9I TTAA 6 cut(s) 482, 653, 663, 1037, 1218, 1350
TscAI CASTG 5 cut(s) 172, 256, 595, 794, 1109
TseFI GTSAC 1 cut(s) 314
TseI GCWGC 2 cut(s) 848, 1312
Tsp45I GTSAC 1 cut(s) 314
TspDTI ATGAA 9 cut(s) 20, 26, 461, 525, 810, 818, 869, 1040, 1044
TspGWI ACGGA 1 cut(s) 232
TspRI CASTG 5 cut(s) 172, 256, 595, 794, 1109
VpaK11BI GGWCC 1 cut(s) 887
XapI RAATTY 2 cut(s) 659, 968
XceI RCATGY 1 cut(s) 1321
XspI CTAG 2 cut(s) 47, 884
Zsp2I ATGCAT 2 cut(s) 1204, 1323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.