Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0004716

Physical Location & Seq

Genomic Coordinates Reverse (-)

1898099 .. 1904131

Gene Structure 6033 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1023400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2598 bp

ATGAAAATGAAGGGCTTTGTGTTTATTGATTTGTTAGCTGTGTTTGTAATCCTTCTTCTATGTGGTTTTGCTGTGGGGAAGGAATGTACAAACATCCCTACTCAGCTTTCATCACATACTTACCGGTATGACCTCTTATCTTCCCACAATGAGACACTCAAGGAGGAAATATTCTCATACTACCACTTGATACCTACCGATGATTCGGCGTGGATGGATTTGCTCCCAAGAAAGATACTGAGGGAAGAAGATGAAGTTGAATGGGGGATGATGTACCGGAAAATGAAAACTTCTGGCGGTTTTGAGGCCTCTGGAAGTTTTCTTAAGGAAGTGCCATTGCATGATGTGAGGTTGCATCCAACTTCACAACATGGAAGGGCACAGCAAACAAATTTGAAGTACTTATTGATGTTGGATTCAGATAGTTTAATTTGGAACTTTCGTCAGACAGCGGGTTTGCCAACCCCTGGAACACCATATAAGGGTTGGGAGGACCCGACTTGTGAGCTTCGGGGTCATTTCGTAGGGCATTACTTGAGTGCAACAGCACAAATGTGGGCTAGCACTCATAATCAGATCCTCAAAGATAAAATGTCAGCACTGGTGGACAGTCTCTCTCTTTGTCAGAAAAAAATTGGCAGCGGATATCTTTCTGCCTTTCCATCTGAAGAATTTGATCGATTTGAAGCTGTTAAGCCTGTCTGGGCTCCTTATTACACCATTCACAAGATATTGGCAGGCCTATTGGATCAGTATATATTTGCTGATAATGCTCAAGCGTTAAAAATGGTCACATGGATGGTTGAGTACTTTTACAACCGTGTTCAGAATGTGATATCCAAGTACAGTGTAGAAAGGCATTATACTGCACTCAATGAAGAAACTGGTGGGATGAATGATGTTCTTTATAAGCTATATGCTATAACGGGAGATGCAAAGCATTTATTGTTGGCTCATCTTTTCGACAAACCATGTTTTCTGGGAGTGCTGGCAGTGCAGGCTGATGACATATCTGGTTTTCACGCTAACACGCATATTCCAATTGTTATCGGTGCTCAAATGCGATATGAAGTCACTGGTGATCCACTTTATAAGGAAATGAGTACTTTCTTTATGAACATTGTTAACTCTTCTCACAGCTATGCAACCGGAGGGACATCAGTGAGCGAGTTCTGGTCCAACCCTAAAAGGTTGGCGAGCACTCTACAAACTGAGAACGAGGAGTCGTGCACAACTTATAACATGCTGAAGGTCTCTCGCCACCTGTTTAGATGGACCAAAGAAACAGCATATGCGGATTATTACGAGCGAGCTCTGACAAATGGCGTGCTAAGCATCCAAAGAGGGACAGAGCCTGGAGTCATGATTTACATGCTTCCACTGGGTCGTGGAAATTCCAAGGCCAGAAGCTACCATGGTTGGGGAAAACCCTTCGAGACTTTCTGGTGCTGCTATGGCACTGGTATTGAATCATTCTCAAAGCTAGGAGATTCCATATATTTTGAAGAGGAGAAAGAAGCTCCTGCTCTTTACATTATCCAGTATATCTCAAGCTTCCTTGATTGGAAATCCGGGCAAATAGCGCTTAATCAGACAGTTGATCATGTCAATTCATGGGATCCTTACCTTAGAGTCGCACTAACGTTTTCTTCAGCAGAGGGGACAGGAAAATCATCTACGTTGAACTTGCGAATTCCAATTTGGACAGATTCAAATGGTGCAAAAGCAGCATTGAATGGTCAAAGTCTGAGTGTTCCAGCTCCAGGTAGTTTCCTAACAGTCACAAGAAAATGGAGTCCTGGTGACATTTTGACCCTTCAGCTGCCCATTAGTCTAAGAACAGAGGCTATTAAAGATGATCGGCCTGAGTACGCTTCGGTTCAAGCAATTCTTTATGGTCCGTATCTGCTTGCGGCTCATACGAGTGGTAACTGGGACATTAATACTGGCTCTGTTAGGTCTCTTTCTGACTGGATAACTCCAATCCCTTTCTCATATAATAACTTCTTGGTTTCTTTATCCCAAGAGTCCGGAAGCTCAAGTTTCGTCATAGCCAACTTAAACGAATCAATTGGATTGGACAAGTTTCCTGAAACTGGCACTGATGCTGCTAATCATGCCACTTTCAGACTCGTTATAAGTGACATGTCCACAGAACTCTCTTCAGCGAATGATGTTATAGGCAAATCGATCATGCTAGAACTGTTTGATCTTCCAGGAAAGGTTGTGGTGCAGCAAGGGATAGACGAGAACCTCGGTATAGCAGACATGTCCACTAGTAGGGACACTTCAGTTTTTCGTTTGGTTCAAGGTTTGGATGGAAAGAACACCACAGTATCCTTAGAGTCCGAAAGCAACCAAGGTTGCTATGTGTATAGCGAAAACGTTGAGGCAAATGCAGACTTGAAGCTCGTCTGCAGTTCAGAGTCATCGAGCGCTGGATTCAATCAGGCAGCCAGCTTTGTGATGAACAATGGAATAAACCAGTATCATCCAATCAGCTTTGTTGCTAAAGGGACACCAAGGAACTTTCTTCTAGCGCCATTGTCGACCTTCAAGGACGAATCTTACACCGCTTATCTCAACATTTTGACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AbfB Glyco_hydro_127

Protein Analysis

866

Amino Acids

96.62

Weight (kDa)

5.5

Isoelectric Point (pI)

35.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-AFase-like_GH127_cat	PF07944	115 - 497	4.9e-114	Beta-L-arabinofuranosidase, GH127 catalytic domain
Glyco_hydro127M	PF20736	510 - 610	3e-15	Beta-L-arabinofuranosidase, GH127 middle domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004716)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12950 AT5G12960
fragaria_vesca	FvH4_3g43250 FvH4_3g43250 FvH4_3g43250
malus_domestica	MD03G1023400.v1.1 MD11G1028500.v1.1
prunus_persica	Prupe.6G022600_v2.0.a1 Prupe.6G022600_v2.0.a1 Prupe.6G022600_v2.0.a1 Prupe.6G022600_v2.0.a1 Prupe.6G022600_v2.0.a1
pyrus_communis	pycom03g02000 pycom11g02200
rosa_chinensis	RchiOBHm_Chr5g0077301 RchiOBHm_Chr5g0077311
rosa_laevigata	RLG00000002632 RLG00000036683 RLG00000036684
rosa_multiflora	Rmu_sc0000433.1_g000041
rosa_roxburghii	Rroxscaffold_1G00004600 Rroxscaffold_1G00004670
rosa_rugosa	Rorug05G0453600
rosa_samantha	Rh5AG508600 Rh5AG508700 Rh5BG529700 Rh5BG529800 Rh5DG542300 Rh5DG542400
rosa_wichuraiana	Rw5G046870 Rw5G046880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	909, 1092, 1239, 2138
AccB7I	CCANNNNNTGG	1 cut(s)	467
AccI	GTMKAC	1 cut(s)	2549
AccIII	TCCGGA	1 cut(s)	2030
AciI	CCGC	6 cut(s)	297, 452, 642, 1295, 1913, 2574
AclI	AACGTT	2 cut(s)	1643, 2385
AclWI	GGATC	5 cut(s)	571, 756, 1076, 1613, 1626
AcsI	RAATTY	4 cut(s)	391, 671, 1393, 1692
AcuI	CTGAAG	6 cut(s)	687, 1268, 1635, 1802, 2148, 2274
AfaI	GTAC	7 cut(s)	88, 275, 401, 809, 845, 1105, 1871
AfeI	AGCGCT	2 cut(s)	1584, 2437
AfiI	CCNNNNNNNGG	6 cut(s)	467, 482, 1420, 1763, 2096, 2280
AflII	CTTAAG	1 cut(s)	323
AflIII	ACRYGT	2 cut(s)	2145, 2268
AgeI	ACCGGT	1 cut(s)	123
AhlI	ACTAGT	1 cut(s)	2276
AjnI	CCWGG	5 cut(s)	466, 1354, 1762, 1798, 2215
AjuI	GAANNNNNNNTTGG	2 cut(s)	1684, 1716
AleI	CACNNNNGTG	1 cut(s)	553
Alw21I	GWGCWC	4 cut(s)	1057, 1202, 1232, 1315
Alw26I	GTCTC	5 cut(s)	146, 617, 1258, 1430, 1965
Alw44I	GTGCAC	1 cut(s)	1228
AlwI	GGATC	5 cut(s)	571, 756, 1076, 1613, 1626
AlwNI	CAGNNNCTG	1 cut(s)	1355
Aor13HI	TCCGGA	1 cut(s)	2030
Aor51HI	AGCGCT	2 cut(s)	1584, 2437
AoxI	GGCC	4 cut(s)	306, 739, 1401, 1862
ApaLI	GTGCAC	1 cut(s)	1228
ApeKI	GCWGC	7 cut(s)	639, 1449, 1727, 1822, 2108, 2233, 2453
ApoI	RAATTY	4 cut(s)	391, 671, 1393, 1692
ArsI	GACNNNNNNTTYG	2 cut(s)	439, 471
AseI	ATTAAT	1 cut(s)	1941
AsiGI	ACCGGT	1 cut(s)	123
AspLEI	GCGC	3 cut(s)	1585, 2438, 2542
AspS9I	GGNCC	4 cut(s)	493, 1176, 1275, 1898
AsuC2I	CCSGG	1 cut(s)	1573
AsuHPI	GGTGA	2 cut(s)	1091, 1814
AsuNHI	GCTAGC	1 cut(s)	560
AvaII	GGWCC	4 cut(s)	493, 1176, 1275, 1898
BaeGI	GKGCMC	2 cut(s)	382, 1232
BaeI	ACNNNNGTAYC	2 cut(s)	2319, 2352
BamHI	GGATCC	1 cut(s)	1618
BanII	GRGCYC	2 cut(s)	709, 1315
BarI	GAAGNNNNNNTAC	4 cut(s)	39, 71, 2272, 2304
Bbv12I	GWGCWC	4 cut(s)	1057, 1202, 1232, 1315
BbvI	GCAGC	7 cut(s)	651, 1436, 1739, 1809, 2095, 2245, 2465
BccI	CCATC	5 cut(s)	208, 670, 793, 1266, 2312
BciT130I	CCWGG	5 cut(s)	468, 1356, 1764, 1800, 2217
BciVI	GTATCC	1 cut(s)	2347
BclI	TGATCA	1 cut(s)	1600
BcnI	CCSGG	1 cut(s)	1573
BcoDI	GTCTC	5 cut(s)	146, 617, 1258, 1430, 1965
BcuI	ACTAGT	1 cut(s)	2276
BfaI	CTAG	5 cut(s)	561, 1484, 2198, 2277, 2537
BfmI	CTRYAG	1 cut(s)	2416
BfoI	RGCGCY	3 cut(s)	1586, 2439, 2543
BfrI	CTTAAG	1 cut(s)	323
BfuI	GTATCC	1 cut(s)	2347
BisI	GCNGC	8 cut(s)	640, 1450, 1728, 1823, 1914, 2109, 2234, 2454
BlpI	GCTNAGC	1 cut(s)	1331
BlsI	GCNGC	8 cut(s)	641, 1451, 1729, 1824, 1915, 2110, 2235, 2455
BmcAI	AGTACT	3 cut(s)	401, 809, 1105
Bme1390I	CCNGG	6 cut(s)	468, 1356, 1573, 1764, 1800, 2217
Bme18I	GGWCC	4 cut(s)	493, 1176, 1275, 1898
BmgT120I	GGNCC	4 cut(s)	493, 1176, 1275, 1898
BmiI	GGNNCC	3 cut(s)	495, 708, 1620
BmrFI	CCNGG	6 cut(s)	468, 1356, 1573, 1764, 1800, 2217
BmrI	ACTGGG	2 cut(s)	1391, 1942
BmsI	GCATC	4 cut(s)	364, 922, 1344, 2095
BmtI	GCTAGC	1 cut(s)	564
BmuI	ACTGGG	2 cut(s)	1391, 1942
BpmI	CTGGAG	2 cut(s)	1377, 1746
Bpu1102I	GCTNAGC	1 cut(s)	1331
BpuEI	CTTGAG	5 cut(s)	143, 556, 759, 1534, 2023
BpuMI	CCSGG	1 cut(s)	1573
Bsa29I	ATCGAT	2 cut(s)	679, 2189
BsaI	GGTCTC	2 cut(s)	1258, 1965
BsaJI	CCNNGG	6 cut(s)	466, 1398, 1414, 2254, 2359, 2522
BsaWI	WCCGGW	4 cut(s)	123, 276, 1148, 2030
BsaXI	ACNNNNNCTCC	2 cut(s)	1786, 1816
Bsc4I	CCNNNNNNNGG	6 cut(s)	467, 482, 1420, 1763, 2096, 2280
Bse118I	RCCGGY	1 cut(s)	123
Bse3DI	GCAATG	1 cut(s)	335
BseAI	TCCGGA	1 cut(s)	2030
BseBI	CCWGG	5 cut(s)	468, 1356, 1764, 1800, 2217
BseCI	ATCGAT	2 cut(s)	679, 2189
BseDI	CCNNGG	6 cut(s)	466, 1398, 1414, 2254, 2359, 2522
BseGI	GGATG	9 cut(s)	93, 219, 273, 355, 804, 897, 1335, 2323, 2491
BseLI	CCNNNNNNNGG	6 cut(s)	467, 482, 1420, 1763, 2096, 2280
BseMI	GCAATG	1 cut(s)	335
BseMII	CTCAG	5 cut(s)	116, 230, 1203, 1739, 1857
BseRI	GAGGAG	2 cut(s)	1235, 1523
BseSI	GKGCMC	2 cut(s)	382, 1232
BseXI	GCAGC	7 cut(s)	651, 1436, 1739, 1809, 2095, 2245, 2465
BsgI	GTGCAG	3 cut(s)	852, 1016, 2252
BshFI	GGCC	4 cut(s)	308, 741, 1403, 1864
BshTI	ACCGGT	1 cut(s)	123
BshVI	ATCGAT	2 cut(s)	679, 2189
BsiHKAI	GWGCWC	4 cut(s)	1057, 1202, 1232, 1315
BsiSI	CCGG	5 cut(s)	124, 277, 1149, 1572, 2031
BslFI	GGGAC	6 cut(s)	1168, 1360, 1675, 1949, 2297, 2530
BslI	CCNNNNNNNGG	6 cut(s)	467, 482, 1420, 1763, 2096, 2280
BsmAI	GTCTC	5 cut(s)	146, 617, 1258, 1430, 1965
BsmFI	GGGAC	6 cut(s)	1168, 1360, 1675, 1949, 2297, 2530
BsnI	GGCC	4 cut(s)	308, 741, 1403, 1864
Bso31I	GGTCTC	2 cut(s)	1258, 1965
Bsp1286I	GDGCHC	6 cut(s)	382, 709, 1057, 1202, 1232, 1315
Bsp13I	TCCGGA	1 cut(s)	2030
Bsp1407I	TGTACA	1 cut(s)	86
Bsp143I	GATC	9 cut(s)	576, 676, 748, 1081, 1600, 1618, 1858, 2190, 2209
Bsp1720I	GCTNAGC	1 cut(s)	1331
Bsp19I	CCATGG	1 cut(s)	1414
BspACI	CCGC	6 cut(s)	297, 452, 642, 1295, 1913, 2574
BspANI	GGCC	4 cut(s)	308, 741, 1403, 1864
BspCNI	CTCAG	5 cut(s)	115, 231, 1204, 1740, 1858
BspDI	ATCGAT	2 cut(s)	679, 2189
BspEI	TCCGGA	1 cut(s)	2030
BspHI	TCATGA	1 cut(s)	1362
BspLI	GGNNCC	3 cut(s)	495, 708, 1620
BspMAI	CTGCAG	1 cut(s)	2420
BspOI	GCTAGC	1 cut(s)	564
BspPI	GGATC	5 cut(s)	571, 756, 1076, 1613, 1626
BspTI	CTTAAG	1 cut(s)	323
BspTNI	GGTCTC	2 cut(s)	1258, 1965
BsrDI	GCAATG	1 cut(s)	335
BsrFI	RCCGGY	1 cut(s)	123
BsrGI	TGTACA	1 cut(s)	86
BssAI	RCCGGY	1 cut(s)	123
BssECI	CCNNGG	6 cut(s)	466, 1398, 1414, 2254, 2359, 2522
BssMI	GATC	9 cut(s)	576, 676, 748, 1081, 1600, 1618, 1858, 2190, 2209
BssT1I	CCWWGG	4 cut(s)	1398, 1414, 2359, 2522
Bst2UI	CCWGG	5 cut(s)	468, 1356, 1764, 1800, 2217
Bst4CI	ACNGT	7 cut(s)	611, 821, 848, 1597, 1780, 2205, 2335
Bst6I	CTCTTC	3 cut(s)	1135, 1500, 2167
BstAFI	CTTAAG	1 cut(s)	323
BstAUI	TGTACA	1 cut(s)	86
BstC8I	GCNNGC	9 cut(s)	562, 739, 990, 999, 1198, 1311, 1328, 1911, 2458
BstDEI	CTNAG	9 cut(s)	102, 239, 1212, 1331, 1628, 1748, 1835, 1866, 2341
BstDSI	CCRYGG	1 cut(s)	1414
BstF5I	GGATG	9 cut(s)	93, 219, 273, 355, 804, 897, 1335, 2323, 2491
BstH2I	RGCGCY	3 cut(s)	1586, 2439, 2543
BstHHI	GCGC	3 cut(s)	1585, 2438, 2542
BstKTI	GATC	9 cut(s)	579, 679, 751, 1084, 1603, 1621, 1861, 2193, 2212
BstMAI	GTCTC	5 cut(s)	146, 617, 1258, 1430, 1965
BstMBI	GATC	9 cut(s)	576, 676, 748, 1081, 1600, 1618, 1858, 2190, 2209
BstMWI	GCNNNNNNNGC	8 cut(s)	770, 994, 998, 1332, 1455, 1582, 1727, 2117
BstNI	CCWGG	5 cut(s)	468, 1356, 1764, 1800, 2217
BstNSI	RCATGY	4 cut(s)	1246, 1375, 2149, 2272
BstSCI	CCNGG	6 cut(s)	466, 1354, 1571, 1762, 1798, 2215
BstSFI	CTRYAG	1 cut(s)	2416
BstSLI	GKGCMC	2 cut(s)	382, 1232
BstV1I	GCAGC	7 cut(s)	651, 1436, 1739, 1809, 2095, 2245, 2465
BstX2I	RGATCY	2 cut(s)	576, 1618
BstYI	RGATCY	2 cut(s)	576, 1618
Bsu15I	ATCGAT	2 cut(s)	679, 2189
BsuI	GTATCC	1 cut(s)	2347
BsuRI	GGCC	4 cut(s)	308, 741, 1403, 1864
BsuTUI	ATCGAT	2 cut(s)	679, 2189
BtgI	CCRYGG	1 cut(s)	1414
BtsCI	GGATG	9 cut(s)	93, 219, 273, 355, 804, 897, 1335, 2323, 2491
BtsI	GCAGTG	1 cut(s)	999
BtsIMutI	CAGTG	8 cut(s)	599, 853, 999, 1074, 1167, 1379, 1458, 2100
Cac8I	GCNNGC	9 cut(s)	562, 739, 990, 999, 1198, 1311, 1328, 1911, 2458
CaiI	CAGNNNCTG	1 cut(s)	1355
CciI	TCATGA	1 cut(s)	1362
CfoI	GCGC	3 cut(s)	1585, 2438, 2542
Cfr10I	RCCGGY	1 cut(s)	123
Cfr13I	GGNCC	4 cut(s)	493, 1176, 1275, 1898
ClaI	ATCGAT	2 cut(s)	679, 2189
Csp6I	GTAC	7 cut(s)	87, 274, 400, 808, 844, 1104, 1870
CspAI	ACCGGT	1 cut(s)	123
CviQI	GTAC	7 cut(s)	87, 274, 400, 808, 844, 1104, 1870
DdeI	CTNAG	9 cut(s)	102, 239, 1212, 1331, 1628, 1748, 1835, 1866, 2341
DpnI	GATC	9 cut(s)	578, 678, 750, 1083, 1602, 1620, 1860, 2192, 2211
DpnII	GATC	9 cut(s)	576, 676, 748, 1081, 1600, 1618, 1858, 2190, 2209
Eam1104I	CTCTTC	3 cut(s)	1135, 1500, 2167
EarI	CTCTTC	3 cut(s)	1135, 1500, 2167
Ecl136II	GAGCTC	1 cut(s)	1313
Eco130I	CCWWGG	4 cut(s)	1398, 1414, 2359, 2522
Eco147I	AGGCCT	2 cut(s)	308, 741
Eco24I	GRGCYC	2 cut(s)	709, 1315
Eco31I	GGTCTC	2 cut(s)	1258, 1965
Eco32I	GATATC	2 cut(s)	647, 837
Eco47I	GGWCC	4 cut(s)	493, 1176, 1275, 1898
Eco47III	AGCGCT	2 cut(s)	1584, 2437
Eco53kI	GAGCTC	1 cut(s)	1313
Eco57I	CTGAAG	6 cut(s)	687, 1268, 1635, 1802, 2148, 2274
EcoICRI	GAGCTC	1 cut(s)	1313
EcoO109I	RGGNCCY	1 cut(s)	493
EcoRI	GAATTC	1 cut(s)	1692
EcoRII	CCWGG	5 cut(s)	466, 1354, 1762, 1798, 2215
EcoRV	GATATC	2 cut(s)	647, 837
EcoT14I	CCWWGG	4 cut(s)	1398, 1414, 2359, 2522
EcoT38I	GRGCYC	2 cut(s)	709, 1315
ErhI	CCWWGG	4 cut(s)	1398, 1414, 2359, 2522
FalI	AAGNNNNNCTT	2 cut(s)	1875, 1907
FaqI	GGGAC	6 cut(s)	1168, 1360, 1675, 1949, 2297, 2530
FauI	CCCGC	1 cut(s)	445
FauNDI	CATATG	1 cut(s)	1291
FbaI	TGATCA	1 cut(s)	1600
FblI	GTMKAC	1 cut(s)	2549
Fnu4HI	GCNGC	8 cut(s)	640, 1450, 1728, 1823, 1914, 2109, 2234, 2454
FokI	GGATG	9 cut(s)	80, 226, 280, 342, 811, 904, 1322, 2330, 2478
FriOI	GRGCYC	2 cut(s)	709, 1315
Fsp4HI	GCNGC	8 cut(s)	640, 1450, 1728, 1823, 1914, 2109, 2234, 2454
FspBI	CTAG	5 cut(s)	561, 1484, 2198, 2277, 2537
GlaI	GCGC	3 cut(s)	1584, 2437, 2541
GluI	GCNGC	8 cut(s)	640, 1450, 1728, 1823, 1914, 2109, 2234, 2454
GsuI	CTGGAG	2 cut(s)	1377, 1746
HaeII	RGCGCY	3 cut(s)	1586, 2439, 2543
HaeIII	GGCC	4 cut(s)	308, 741, 1403, 1864
HapII	CCGG	5 cut(s)	124, 277, 1149, 1572, 2031
HhaI	GCGC	3 cut(s)	1585, 2438, 2542
Hin6I	GCGC	3 cut(s)	1583, 2436, 2540
HinP1I	GCGC	3 cut(s)	1583, 2436, 2540
HincII	GTYRAC	2 cut(s)	1126, 2550
HindII	GTYRAC	2 cut(s)	1126, 2550
HindIII	AAGCTT	1 cut(s)	1552
HpaI	GTTAAC	1 cut(s)	1126
HpaII	CCGG	5 cut(s)	124, 277, 1149, 1572, 2031
HphI	GGTGA	2 cut(s)	1091, 1814
Hpy166II	GTNNAC	6 cut(s)	607, 1126, 1230, 2151, 2274, 2550
Hpy188III	TCNNGA	5 cut(s)	312, 1363, 1435, 2031, 2090
Hpy8I	GTNNAC	6 cut(s)	607, 1126, 1230, 2151, 2274, 2550
HpyAV	CCTTC	8 cut(s)	4, 62, 73, 369, 1243, 1441, 1826, 2563
HpyCH4III	ACNGT	7 cut(s)	611, 821, 848, 1597, 1780, 2205, 2335
HpyCH4IV	ACGT	3 cut(s)	1643, 1679, 2385
HpyF10VI	GCNNNNNNNGC	8 cut(s)	770, 994, 998, 1332, 1455, 1582, 1727, 2117
HpyF3I	CTNAG	9 cut(s)	102, 239, 1212, 1331, 1628, 1748, 1835, 1866, 2341
HpySE526I	ACGT	3 cut(s)	1643, 1679, 2385
HspAI	GCGC	3 cut(s)	1583, 2436, 2540
Kpn2I	TCCGGA	1 cut(s)	2030
Ksp22I	TGATCA	1 cut(s)	1600
KspAI	GTTAAC	1 cut(s)	1126
Kzo9I	GATC	9 cut(s)	576, 676, 748, 1081, 1600, 1618, 1858, 2190, 2209
LmnI	GCTCC	4 cut(s)	228, 712, 1525, 1765
Lsp1109I	GCAGC	7 cut(s)	651, 1436, 1739, 1809, 2095, 2245, 2465
LweI	GCATC	4 cut(s)	364, 922, 1344, 2095
MaeI	CTAG	5 cut(s)	561, 1484, 2198, 2277, 2537
MaeII	ACGT	3 cut(s)	1643, 1679, 2385
MaeIII	GTNAC	6 cut(s)	790, 1072, 1780, 1802, 1928, 2141
MalI	GATC	9 cut(s)	578, 678, 750, 1083, 1602, 1620, 1860, 2192, 2211
MboI	GATC	9 cut(s)	576, 676, 748, 1081, 1600, 1618, 1858, 2190, 2209
MfeI	CAATTG	2 cut(s)	1041, 2070
MflI	RGATCY	2 cut(s)	576, 1618
MhlI	GDGCHC	6 cut(s)	382, 709, 1057, 1202, 1232, 1315
MlyI	GAGTC	8 cut(s)	1232, 1368, 1641, 1804, 2036, 2124, 2354, 2435
MmeI	TCCRAC	3 cut(s)	383, 393, 1203
MroI	TCCGGA	1 cut(s)	2030
MseI	TTAA	9 cut(s)	324, 428, 693, 782, 1125, 1587, 1851, 1941, 2060
MslI	CAYNNNNRTG	4 cut(s)	553, 797, 1140, 1923
MspA1I	CMGCKG	3 cut(s)	452, 642, 1822
MspCI	CTTAAG	1 cut(s)	323
MspI	CCGG	5 cut(s)	124, 277, 1149, 1572, 2031
MspR9I	CCNGG	6 cut(s)	468, 1356, 1573, 1764, 1800, 2217
MunI	CAATTG	2 cut(s)	1041, 2070
MvaI	CCWGG	5 cut(s)	468, 1356, 1764, 1800, 2217
MwoI	GCNNNNNNNGC	8 cut(s)	770, 994, 998, 1332, 1455, 1582, 1727, 2117
NciI	CCSGG	1 cut(s)	1573
NcoI	CCATGG	1 cut(s)	1414
NdeI	CATATG	1 cut(s)	1291
NdeII	GATC	9 cut(s)	576, 676, 748, 1081, 1600, 1618, 1858, 2190, 2209
NheI	GCTAGC	1 cut(s)	560
NlaIV	GGNNCC	3 cut(s)	495, 708, 1620
NmuCI	GTSAC	5 cut(s)	790, 1072, 1780, 1802, 2141
NspI	RCATGY	4 cut(s)	1246, 1375, 2149, 2272
OliI	CACNNNNGTG	1 cut(s)	553
PagI	TCATGA	1 cut(s)	1362
PceI	AGGCCT	2 cut(s)	308, 741
PciI	ACATGT	2 cut(s)	2145, 2268
PfeI	GAWTC	8 cut(s)	203, 416, 1469, 1490, 1709, 2066, 2442, 2564
PflMI	CCANNNNNTGG	1 cut(s)	467
PfoI	TCCNGGA	1 cut(s)	2215
PinAI	ACCGGT	1 cut(s)	123
PkrI	GCNGC	8 cut(s)	641, 1451, 1729, 1824, 1915, 2110, 2235, 2455
PleI	GAGTC	8 cut(s)	1231, 1367, 1640, 1803, 2035, 2124, 2353, 2434
PpsI	GAGTC	8 cut(s)	1231, 1367, 1640, 1803, 2035, 2124, 2353, 2434
PpuMI	RGGWCCY	1 cut(s)	493
PscI	ACATGT	2 cut(s)	2145, 2268
PshBI	ATTAAT	1 cut(s)	1941
PsiI	TTATAA	4 cut(s)	909, 1092, 1239, 2138
Psp124BI	GAGCTC	1 cut(s)	1315
Psp1406I	AACGTT	2 cut(s)	1643, 2385
Psp5II	RGGWCCY	1 cut(s)	493
Psp6I	CCWGG	5 cut(s)	466, 1354, 1762, 1798, 2215
PspGI	CCWGG	5 cut(s)	466, 1354, 1762, 1798, 2215
PspN4I	GGNNCC	3 cut(s)	495, 708, 1620
PspPI	GGNCC	4 cut(s)	493, 1176, 1275, 1898
PspPPI	RGGWCCY	1 cut(s)	493
PstI	CTGCAG	1 cut(s)	2420
PstNI	CAGNNNCTG	1 cut(s)	1355
PsuI	RGATCY	2 cut(s)	576, 1618
PvuII	CAGCTG	1 cut(s)	1822
RsaI	GTAC	7 cut(s)	88, 275, 401, 809, 845, 1105, 1871
RsaNI	GTAC	7 cut(s)	87, 274, 400, 808, 844, 1104, 1870
RseI	CAYNNNNRTG	4 cut(s)	553, 797, 1140, 1923
SacI	GAGCTC	1 cut(s)	1315
SalI	GTCGAC	1 cut(s)	2548
SaqAI	TTAA	9 cut(s)	324, 428, 693, 782, 1125, 1587, 1851, 1941, 2060
SatI	GCNGC	8 cut(s)	640, 1450, 1728, 1823, 1914, 2109, 2234, 2454
Sau3AI	GATC	9 cut(s)	576, 676, 748, 1081, 1600, 1618, 1858, 2190, 2209
Sau96I	GGNCC	4 cut(s)	493, 1176, 1275, 1898
ScaI	AGTACT	3 cut(s)	401, 809, 1105
SchI	GAGTC	8 cut(s)	1232, 1368, 1641, 1804, 2036, 2124, 2354, 2435
ScrFI	CCNGG	6 cut(s)	468, 1356, 1573, 1764, 1800, 2217
SduI	GDGCHC	6 cut(s)	382, 709, 1057, 1202, 1232, 1315
SfaNI	GCATC	4 cut(s)	364, 922, 1344, 2095
SfcI	CTRYAG	1 cut(s)	2416
SinI	GGWCC	4 cut(s)	493, 1176, 1275, 1898
SmiMI	CAYNNNNRTG	4 cut(s)	553, 797, 1140, 1923
SmlI	CTYRAG	6 cut(s)	158, 323, 535, 774, 1549, 2038
SmoI	CTYRAG	6 cut(s)	158, 323, 535, 774, 1549, 2038
SpeI	ACTAGT	1 cut(s)	2276
SseBI	AGGCCT	2 cut(s)	308, 741
SsiI	CCGC	6 cut(s)	297, 452, 642, 1295, 1913, 2574
SspI	AATATT	1 cut(s)	171
SspMI	CTAG	5 cut(s)	561, 1484, 2198, 2277, 2537
SstI	GAGCTC	1 cut(s)	1315
StuI	AGGCCT	2 cut(s)	308, 741
StyD4I	CCNGG	6 cut(s)	466, 1354, 1571, 1762, 1798, 2215
StyI	CCWWGG	4 cut(s)	1398, 1414, 2359, 2522
TaaI	ACNGT	7 cut(s)	611, 821, 848, 1597, 1780, 2205, 2335
TaiI	ACGT	3 cut(s)	1646, 1682, 2388
TaqI	TCGA	6 cut(s)	679, 963, 1434, 2189, 2432, 2549
TatI	WGTACW	5 cut(s)	86, 399, 807, 843, 1103
TauI	GCSGC	1 cut(s)	1916
TfiI	GAWTC	8 cut(s)	203, 416, 1469, 1490, 1709, 2066, 2442, 2564
Tru1I	TTAA	9 cut(s)	324, 428, 693, 782, 1125, 1587, 1851, 1941, 2060
Tru9I	TTAA	9 cut(s)	324, 428, 693, 782, 1125, 1587, 1851, 1941, 2060
TscAI	CASTG	8 cut(s)	606, 853, 999, 1081, 1167, 1386, 1465, 2107
TseFI	GTSAC	5 cut(s)	790, 1072, 1780, 1802, 2141
TseI	GCWGC	7 cut(s)	639, 1449, 1727, 1822, 2108, 2233, 2453
Tsp45I	GTSAC	5 cut(s)	790, 1072, 1780, 1802, 2141
TspGWI	ACGGA	1 cut(s)	1890
TspRI	CASTG	8 cut(s)	606, 853, 999, 1081, 1167, 1386, 1465, 2107
Van91I	CCANNNNNTGG	1 cut(s)	467
Vha464I	CTTAAG	1 cut(s)	323
VneI	GTGCAC	1 cut(s)	1228
VpaK11BI	GGWCC	4 cut(s)	493, 1176, 1275, 1898
VspI	ATTAAT	1 cut(s)	1941
XapI	RAATTY	4 cut(s)	391, 671, 1393, 1692
XceI	RCATGY	4 cut(s)	1246, 1375, 2149, 2272
XcmI	CCANNNNNNNNNTGG	1 cut(s)	483
XmiI	GTMKAC	1 cut(s)	2549
XspI	CTAG	5 cut(s)	561, 1484, 2198, 2277, 2537
ZrmI	AGTACT	3 cut(s)	401, 809, 1105

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.