Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0004716

Physical Location & Seq

Genomic Coordinates Reverse (-)

81209972 .. 81215332

Gene Structure 5361 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G046870.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2574 bp

ATGGAAATGAAGGGTTTTGGGTTTATTAATATGTCATCTGTGTTTGTGATTTTTCTGGTATGTGTATGTGGATGTGGTTTGGCTGAGGCTAGGGAGTGTACAAACGCTGTTTATGCTTCCAAAAACGAAACTTTGAAGCATATGGTGCTCTCACACTATCACTTGACACGAAGTGATGATTCTGCTCGGTTGAAGTTGCACCCAAGAAAGATGTTGAGGGAGGAAGATGGATATGAGTGGTCAGTGCTGTACCGGAAGATGAAAACCTCCGGTGGTGGTCCTGGGGCCCCCGGAGGTCTTCTCAAGGAAGTGTCATTGCATGATGTGAGGTTGGATCCAACTTCACAACATGGGAAGGCACAGCAAACAAATCTAGAGTACCTGTTGATGTTGGATTCGGATAATTTACTTTGGAACTTTCGAAAGACAGCTGGTTTAGAAACCCCTGGGGAACCATATGATGCTAAAAGTTCGTGGGAGGCATCGACTAGTGAACTGCGGGGTCATTTTGTAGGGCATTATTTGAGTGCTTCAGCAATGATGTGGGCTAGCACTCATAATGATACTCTCAAAGAGAAGATGTCCGGGATTGTGTCCAGTCTTTCTCTTTGTCAGAAGAAAATTGGCAGTGGATATCTTTCGGCTTTTCCAACTGAGGAATTTGATCGCGTTGAAGCTCATACACCTGTCTGGGTTCCTTATTATACCATTCACAAGATCTTGGCAGGCCTGTTGGATCAGTATACACTTGCCGGTAGTGATCAAGCTTTTAGTATGATTAAATGGATGGTTGAGTACTTTTACAAACGTGTTCAGAATGTGATATCGAAGTACAGTATAGCAAAACATTATCTGTCACTCGATGCAGAACCCGGTGGCATGAATGACATTCTTTATAAATTATATGCTATCACGGGAGATACAAAGCATTTATTGTTGGCTCATCTTTTTGACAAACCCTGCTTTCTAGGATTGTTGGCGGTGCAGGCTAATGACATATCTTATTTTCACGCTAACACGCATATTGCAATTGTCATTGGTGCTCAAAAGCGCTATGAAGTCACTGGTGATCCACTTTATAAGGAAATGGGGATTTTCTTTATGGACATTGTTAACTCTTCTCACAGCTATGCAACAGGAGGGACATCAGTCAGCGAGCATTGGCAGGAACCTAAAAGATTGGGGGCCTCTCTAGGATCAGACAATGAGGAATCGTGCTCAACTTATAACATGCTGAAGGTCTCTCGCCACCTGTTTAGATGGACCAAAGAAACTCCATATGCGGATTATTATGAGCGAGCTCTAACAAATGGTGTGCTAGGTATCCAAAGAGGAACTGAGCCTGGAGTTATGATCTACTTCCTCCCACTAGGCCGTGGACAGTCCAAGTCCGTGAGTTCTTTTGGTTGGGGATCGCCATTTGGCCATTTCTGGTGCTGCTATGGTACTGGGATTGAATCATTCTCAAAGTTAGGAGATTCCATATATTTTGAAGAGGAAGGGGAAACTCCTGGTCTTTACATTATCCAGTATATATCAAGCTTGCTTGATTGGAAATCTGGGCAAGTCGTACTTAATCAGACAGTTGGTAACATCAATTCCTGGGATCCTTACCTTAGAATCACGTTAACATTTTCTTCCACGAAGGTACAAATCATATTGACTATGGCTTCAACATTAACAATCTTGTATGGGACAGGCAAATCATCAACCGTGAACTTGCGAATGCCAATTTGGACAGATTCAGATGGTGCAAAGGCAGCATTGAATGGTCGAAATCTGCATCCCTCATCTCCAGGTAGTTTCCTACCAATCCCAAATAAATGGAGCCATGGTGACACAGTAACTCTTGAGCTACCCATTAGTCTGAGAACAGAAGCTATTAAAGACGATCAGGCAGAGTATGCTTCAGTCCAAGCAATTCTTTATGGTCCAGTTCTGCTTGCAGCTCATACCAGTGGTAACTGGGACATCCAAACTAAGTCAACTAATCTTTCAGACTGGATAACTCCACTCCCTTCCTCGTACAATAATTATTTGTCGATCGAACTAGACATCTTTCCGGATGTAGGCACTGTTACTGCTGTTCATGCCACTTTCAGACTCATTTTAGATGACTTATCAACAAAAGATTTCAAAGCGCAAGATGCTATTGGAAAATCAGTCATGCTAGAACTGTTTGATCTTCCTGGGAAGGTTCTTGGGCATCAAGAGAAAGACAAGAACCTTGTGATAGCAGATTCTTCAAGTAGCAAGGAGAACATAGTTTTTCGCTTGGTTCCAGGTTTGGATGGAAAGTCTGCCTCGATCTCCTTGGAGTCAGAGAGTGACAAGGGTTGCTTTGTGTATAGCTCGGAGTTGGAGGTTAATGCAGCCTTGAAGCTCGGTTGCAGTTCAAAATTGTCAAACGATGGATTCAAACAGGCAGCCAGCTTTGTAATGAAGAGTGGAATAAGTCAGTATCATCCTATCAGCTTTGTTGCCCAAGGGAAGCATCGGAATTTTCTTCTACAACCATTGTCAAGCTTCATGGATGAAACTTATACTGTCTATCTCAACATTACAACGGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AbfB Glyco_hydro_127

Protein Analysis

857

Amino Acids

95.15

Weight (kDa)

6.09

Isoelectric Point (pI)

33.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-AFase-like_GH127_cat	PF07944	108 - 493	1.3e-103	Beta-L-arabinofuranosidase, GH127 catalytic domain
Glyco_hydro127M	PF20736	506 - 621	1.3e-10	Beta-L-arabinofuranosidase, GH127 middle domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004716)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12950 AT5G12960
fragaria_vesca	FvH4_3g43250 FvH4_3g43250 FvH4_3g43250
malus_domestica	MD03G1023400.v1.1 MD11G1028500.v1.1
prunus_persica	Prupe.6G022600_v2.0.a1 Prupe.6G022600_v2.0.a1 Prupe.6G022600_v2.0.a1 Prupe.6G022600_v2.0.a1 Prupe.6G022600_v2.0.a1
pyrus_communis	pycom03g02000 pycom11g02200
rosa_chinensis	RchiOBHm_Chr5g0077301 RchiOBHm_Chr5g0077311
rosa_laevigata	RLG00000002632 RLG00000036683 RLG00000036684
rosa_multiflora	Rmu_sc0000433.1_g000041
rosa_roxburghii	Rroxscaffold_1G00004600 Rroxscaffold_1G00004670
rosa_rugosa	Rorug05G0453600
rosa_samantha	Rh5AG508600 Rh5AG508700 Rh5BG529700 Rh5BG529800 Rh5DG542300 Rh5DG542400
rosa_wichuraiana	Rw5G046870 Rw5G046880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	897, 1080, 1227
AccI	GTMKAC	1 cut(s)	743
AccII	CGCG	1 cut(s)	669
AccIII	TCCGGA	1 cut(s)	2062
AciI	CCGC	3 cut(s)	499, 980, 1283
AclWI	GGATC	8 cut(s)	329, 342, 744, 1064, 1204, 1420, 1601, 1614
AcoI	YGGCCR	1 cut(s)	1423
AcsI	RAATTY	2 cut(s)	659, 2500
AcuI	CTGAAG	3 cut(s)	516, 1256, 1893
AdeI	CACNNNGTG	1 cut(s)	173
AfaI	GTAC	9 cut(s)	100, 251, 380, 797, 833, 1447, 1572, 1650, 2027
AfeI	AGCGCT	1 cut(s)	1052
AfiI	CCNNNNNNNGG	1 cut(s)	2069
AflIII	ACRYGT	1 cut(s)	808
AhlI	ACTAGT	1 cut(s)	488
AjnI	CCWGG	8 cut(s)	280, 445, 1342, 1510, 1601, 1795, 2188, 2281
AjuI	GAANNNNNNNTTGG	2 cut(s)	1717, 1749
Alw21I	GWGCWC	4 cut(s)	150, 1045, 1220, 1303
Alw26I	GTCTC	1 cut(s)	1246
AlwI	GGATC	8 cut(s)	329, 342, 744, 1064, 1204, 1420, 1601, 1614
Aor13HI	TCCGGA	1 cut(s)	2062
Aor51HI	AGCGCT	1 cut(s)	1052
AoxI	GGCC	5 cut(s)	285, 727, 1185, 1372, 1423
ApaI	GGGCCC	1 cut(s)	289
ApeKI	GCWGC	5 cut(s)	1437, 1760, 1946, 2372, 2426
ApoI	RAATTY	2 cut(s)	659, 2500
AseI	ATTAAT	1 cut(s)	27
AspLEI	GCGC	2 cut(s)	1053, 2143
AspS9I	GGNCC	6 cut(s)	278, 285, 286, 1185, 1263, 1931
AsuC2I	CCSGG	3 cut(s)	291, 586, 873
AsuHPI	GGTGA	2 cut(s)	1079, 1847
AsuII	TTCGAA	1 cut(s)	421
AsuNHI	GCTAGC	1 cut(s)	548
AvaII	GGWCC	3 cut(s)	278, 1263, 1931
BaeGI	GKGCMC	1 cut(s)	289
BalI	TGGCCA	1 cut(s)	1425
BamHI	GGATCC	2 cut(s)	334, 1606
BanII	GRGCYC	2 cut(s)	289, 1303
BbsI	GAAGAC	1 cut(s)	290
Bbv12I	GWGCWC	4 cut(s)	150, 1045, 1220, 1303
BbvCI	CCTCAGC	1 cut(s)	84
BbvI	GCAGC	5 cut(s)	1424, 1772, 1958, 2384, 2438
BccI	CCATC	6 cut(s)	221, 781, 1254, 1742, 2285, 2405
BceAI	ACGGC	1 cut(s)	1359
BciT130I	CCWGG	8 cut(s)	282, 447, 1344, 1512, 1603, 1797, 2190, 2283
BciVI	GTATCC	1 cut(s)	1334
BclI	TGATCA	1 cut(s)	760
BcnI	CCSGG	3 cut(s)	291, 586, 873
BcoDI	GTCTC	1 cut(s)	1246
BcuI	ACTAGT	1 cut(s)	488
BfoI	RGCGCY	1 cut(s)	1054
BfuI	GTATCC	1 cut(s)	1334
BglII	AGATCT	1 cut(s)	717
BisI	GCNGC	5 cut(s)	1438, 1761, 1947, 2373, 2427
BlsI	GCNGC	5 cut(s)	1439, 1762, 1948, 2374, 2428
BmcAI	AGTACT	1 cut(s)	797
Bme18I	GGWCC	3 cut(s)	278, 1263, 1931
BmgT120I	GGNCC	6 cut(s)	278, 285, 286, 1185, 1263, 1931
BmrI	ACTGGG	2 cut(s)	1458, 1975
BmsI	GCATC	7 cut(s)	451, 491, 853, 1792, 2137, 2215, 2503
BmtI	GCTAGC	1 cut(s)	552
BmuI	ACTGGG	2 cut(s)	1458, 1975
BoxI	GACNNNNGTC	1 cut(s)	1148
BpiI	GAAGAC	1 cut(s)	290
BplI	GAGNNNNNCTC	2 cut(s)	285, 317
BpmI	CTGGAG	2 cut(s)	1365, 1779
Bpu10I	CCTNAGC	1 cut(s)	84
Bpu14I	TTCGAA	1 cut(s)	421
BpuEI	CTTGAG	2 cut(s)	287, 1871
BpuMI	CCSGG	3 cut(s)	291, 586, 873
BsaI	GGTCTC	1 cut(s)	1246
BsaWI	WCCGGW	3 cut(s)	252, 269, 2062
BsaXI	ACNNNNNCTCC	2 cut(s)	1819, 1849
Bsc4I	CCNNNNNNNGG	1 cut(s)	2069
Bse118I	RCCGGY	1 cut(s)	752
Bse1I	ACTGG	8 cut(s)	597, 1069, 1453, 1528, 1934, 1956, 1970, 2006
Bse3DI	GCAATG	2 cut(s)	314, 543
BseAI	TCCGGA	1 cut(s)	2062
BseBI	CCWGG	8 cut(s)	282, 447, 1344, 1512, 1603, 1797, 2190, 2283
BseGI	GGATG	8 cut(s)	77, 792, 1783, 1971, 2071, 2296, 2464, 2539
BseLI	CCNNNNNNNGG	1 cut(s)	2069
BseMI	GCAATG	2 cut(s)	314, 543
BseMII	CTCAG	4 cut(s)	75, 645, 1329, 1859
BseNI	ACTGG	8 cut(s)	597, 1069, 1453, 1528, 1934, 1956, 1970, 2006
BseSI	GKGCMC	1 cut(s)	289
BseXI	GCAGC	5 cut(s)	1424, 1772, 1958, 2384, 2438
BsgI	GTGCAG	1 cut(s)	1004
Bsh1236I	CGCG	1 cut(s)	669
Bsh1285I	CGRYCG	1 cut(s)	2046
BshFI	GGCC	5 cut(s)	287, 729, 1187, 1374, 1425
BsiEI	CGRYCG	1 cut(s)	2046
BsiHKAI	GWGCWC	4 cut(s)	150, 1045, 1220, 1303
BsiSI	CCGG	7 cut(s)	253, 270, 291, 585, 753, 873, 2063
BslFI	GGGAC	3 cut(s)	1156, 1708, 1982
BslI	CCNNNNNNNGG	1 cut(s)	2069
BsmAI	GTCTC	1 cut(s)	1246
BsmFI	GGGAC	3 cut(s)	1156, 1708, 1982
BsmI	GAATGC	1 cut(s)	1731
BsnI	GGCC	5 cut(s)	287, 729, 1187, 1374, 1425
Bso31I	GGTCTC	1 cut(s)	1246
Bsp119I	TTCGAA	1 cut(s)	421
Bsp120I	GGGCCC	1 cut(s)	285
Bsp1286I	GDGCHC	5 cut(s)	150, 289, 1045, 1220, 1303
Bsp13I	TCCGGA	1 cut(s)	2062
Bsp1407I	TGTACA	1 cut(s)	98
Bsp19I	CCATGG	1 cut(s)	1831
BspACI	CCGC	3 cut(s)	499, 980, 1283
BspANI	GGCC	5 cut(s)	287, 729, 1187, 1374, 1425
BspCNI	CTCAG	4 cut(s)	76, 646, 1330, 1860
BspEI	TCCGGA	1 cut(s)	2062
BspFNI	CGCG	1 cut(s)	669
BspOI	GCTAGC	1 cut(s)	552
BspPI	GGATC	8 cut(s)	329, 342, 744, 1064, 1204, 1420, 1601, 1614
BspT104I	TTCGAA	1 cut(s)	421
BspTNI	GGTCTC	1 cut(s)	1246
BsrDI	GCAATG	2 cut(s)	314, 543
BsrFI	RCCGGY	1 cut(s)	752
BsrGI	TGTACA	1 cut(s)	98
BsrI	ACTGG	8 cut(s)	597, 1069, 1453, 1528, 1934, 1956, 1970, 2006
BssAI	RCCGGY	1 cut(s)	752
BssNAI	GTATAC	1 cut(s)	744
BssT1I	CCWWGG	3 cut(s)	1831, 2313, 2485
Bst1107I	GTATAC	1 cut(s)	744
Bst2UI	CCWGG	8 cut(s)	282, 447, 1344, 1512, 1603, 1797, 2190, 2283
Bst4CI	ACNGT	9 cut(s)	836, 1383, 1585, 1714, 1843, 2077, 2178, 2548, 2569
Bst6I	CTCTTC	3 cut(s)	1123, 1488, 2438
BstAPI	GCANNNNNTGC	2 cut(s)	145, 1904
BstAUI	TGTACA	1 cut(s)	98
BstBI	TTCGAA	1 cut(s)	421
BstC8I	GCNNGC	8 cut(s)	550, 727, 987, 1157, 1299, 1544, 1944, 2431
BstDEI	CTNAG	6 cut(s)	84, 654, 1338, 1616, 1868, 1980
BstDSI	CCRYGG	2 cut(s)	1375, 1831
BstF5I	GGATG	8 cut(s)	77, 792, 1783, 1971, 2071, 2296, 2464, 2539
BstFNI	CGCG	1 cut(s)	669
BstH2I	RGCGCY	1 cut(s)	1054
BstHHI	GCGC	2 cut(s)	1053, 2143
BstMAI	GTCTC	1 cut(s)	1246
BstMCI	CGRYCG	1 cut(s)	2046
BstMWI	GCNNNNNNNGC	7 cut(s)	113, 145, 986, 1760, 1904, 2090, 2147
BstNI	CCWGG	8 cut(s)	282, 447, 1344, 1512, 1603, 1797, 2190, 2283
BstNSI	RCATGY	1 cut(s)	1234
BstPAI	GACNNNNGTC	1 cut(s)	1148
BstSLI	GKGCMC	1 cut(s)	289
BstUI	CGCG	1 cut(s)	669
BstV1I	GCAGC	5 cut(s)	1424, 1772, 1958, 2384, 2438
BstV2I	GAAGAC	1 cut(s)	290
BstX2I	RGATCY	3 cut(s)	334, 717, 1606
BstXI	CCANNNNNNTGG	1 cut(s)	1824
BstYI	RGATCY	3 cut(s)	334, 717, 1606
BstZ17I	GTATAC	1 cut(s)	744
BsuI	GTATCC	1 cut(s)	1334
BsuRI	GGCC	5 cut(s)	287, 729, 1187, 1374, 1425
BtgI	CCRYGG	2 cut(s)	1375, 1831
BtsCI	GGATG	8 cut(s)	77, 792, 1783, 1971, 2071, 2296, 2464, 2539
BtsI	GCAGTG	1 cut(s)	634
BtsIMutI	CAGTG	5 cut(s)	249, 634, 1062, 1963, 2073
Cac8I	GCNNGC	8 cut(s)	550, 727, 987, 1157, 1299, 1544, 1944, 2431
CfoI	GCGC	2 cut(s)	1053, 2143
Cfr10I	RCCGGY	1 cut(s)	752
Cfr13I	GGNCC	6 cut(s)	278, 285, 286, 1185, 1263, 1931
Csp6I	GTAC	9 cut(s)	99, 250, 379, 796, 832, 1446, 1571, 1649, 2026
CviAII	CATG	8 cut(s)	320, 350, 880, 1231, 1832, 2090, 2167, 2530
CviQI	GTAC	9 cut(s)	99, 250, 379, 796, 832, 1446, 1571, 1649, 2026
DdeI	CTNAG	6 cut(s)	84, 654, 1338, 1616, 1868, 1980
DraIII	CACNNNGTG	1 cut(s)	173
EaeI	YGGCCR	1 cut(s)	1423
Eam1104I	CTCTTC	3 cut(s)	1123, 1488, 2438
EarI	CTCTTC	3 cut(s)	1123, 1488, 2438
Ecl136II	GAGCTC	1 cut(s)	1301
Eco130I	CCWWGG	3 cut(s)	1831, 2313, 2485
Eco147I	AGGCCT	1 cut(s)	729
Eco24I	GRGCYC	2 cut(s)	289, 1303
Eco31I	GGTCTC	1 cut(s)	1246
Eco32I	GATATC	2 cut(s)	635, 825
Eco47I	GGWCC	3 cut(s)	278, 1263, 1931
Eco47III	AGCGCT	1 cut(s)	1052
Eco53kI	GAGCTC	1 cut(s)	1301
Eco57I	CTGAAG	3 cut(s)	516, 1256, 1893
EcoICRI	GAGCTC	1 cut(s)	1301
EcoO109I	RGGNCCY	3 cut(s)	285, 286, 1185
EcoRII	CCWGG	8 cut(s)	280, 445, 1342, 1510, 1601, 1795, 2188, 2281
EcoRV	GATATC	2 cut(s)	635, 825
EcoT14I	CCWWGG	3 cut(s)	1831, 2313, 2485
EcoT38I	GRGCYC	2 cut(s)	289, 1303
ErhI	CCWWGG	3 cut(s)	1831, 2313, 2485
FaeI	CATG	8 cut(s)	323, 353, 883, 1234, 1835, 2093, 2170, 2533
FalI	AAGNNNNNCTT	6 cut(s)	1557, 1589, 1908, 1940, 2324, 2356
FaqI	GGGAC	3 cut(s)	1156, 1708, 1982
FatI	CATG	8 cut(s)	319, 349, 879, 1230, 1831, 2089, 2166, 2529
FauI	CCCGC	1 cut(s)	492
FauNDI	CATATG	3 cut(s)	141, 457, 1279
FbaI	TGATCA	1 cut(s)	760
FblI	GTMKAC	1 cut(s)	743
Fnu4HI	GCNGC	5 cut(s)	1438, 1761, 1947, 2373, 2427
FokI	GGATG	8 cut(s)	84, 799, 1770, 1958, 2078, 2303, 2451, 2546
FriOI	GRGCYC	2 cut(s)	289, 1303
Fsp4HI	GCNGC	5 cut(s)	1438, 1761, 1947, 2373, 2427
GlaI	GCGC	2 cut(s)	1052, 2142
GluI	GCNGC	5 cut(s)	1438, 1761, 1947, 2373, 2427
GsuI	CTGGAG	2 cut(s)	1365, 1779
HaeII	RGCGCY	1 cut(s)	1054
HaeIII	GGCC	5 cut(s)	287, 729, 1187, 1374, 1425
HapII	CCGG	7 cut(s)	253, 270, 291, 585, 753, 873, 2063
HhaI	GCGC	2 cut(s)	1053, 2143
Hin1II	CATG	8 cut(s)	323, 353, 883, 1234, 1835, 2093, 2170, 2533
Hin6I	GCGC	2 cut(s)	1051, 2141
HinP1I	GCGC	2 cut(s)	1051, 2141
HincII	GTYRAC	3 cut(s)	1114, 1629, 1986
HindII	GTYRAC	3 cut(s)	1114, 1629, 1986
HindIII	AAGCTT	3 cut(s)	765, 1540, 2524
HpaI	GTTAAC	2 cut(s)	1114, 1629
HpaII	CCGG	7 cut(s)	253, 270, 291, 585, 753, 873, 2063
HphI	GGTGA	2 cut(s)	1079, 1847
Hpy166II	GTNNAC	8 cut(s)	99, 494, 744, 1114, 1379, 1629, 1717, 1986
Hpy188III	TCNNGA	4 cut(s)	374, 1850, 2063, 2210
Hpy8I	GTNNAC	8 cut(s)	99, 494, 744, 1114, 1379, 1629, 1717, 1986
HpyAV	CCTTC	7 cut(s)	4, 349, 1231, 1493, 1639, 2028, 2188
HpyCH4III	ACNGT	9 cut(s)	836, 1383, 1585, 1714, 1843, 2077, 2178, 2548, 2569
HpyCH4IV	ACGT	2 cut(s)	808, 1625
HpyF10VI	GCNNNNNNNGC	7 cut(s)	113, 145, 986, 1760, 1904, 2090, 2147
HpyF3I	CTNAG	6 cut(s)	84, 654, 1338, 1616, 1868, 1980
HpySE526I	ACGT	2 cut(s)	808, 1625
Hsp92II	CATG	8 cut(s)	323, 353, 883, 1234, 1835, 2093, 2170, 2533
HspAI	GCGC	2 cut(s)	1051, 2141
Kpn2I	TCCGGA	1 cut(s)	2062
Ksp22I	TGATCA	1 cut(s)	760
KspAI	GTTAAC	2 cut(s)	1114, 1629
LmnI	GCTCC	1 cut(s)	1827
Lsp1109I	GCAGC	5 cut(s)	1424, 1772, 1958, 2384, 2438
LweI	GCATC	7 cut(s)	451, 491, 853, 1792, 2137, 2215, 2503
MaeII	ACGT	2 cut(s)	808, 1625
MaeIII	GTNAC	8 cut(s)	855, 1060, 1589, 1835, 1843, 1961, 2077, 2327
MfeI	CAATTG	1 cut(s)	1029
MflI	RGATCY	3 cut(s)	334, 717, 1606
MhlI	GDGCHC	5 cut(s)	150, 289, 1045, 1220, 1303
MlsI	TGGCCA	1 cut(s)	1425
MluNI	TGGCCA	1 cut(s)	1425
MlyI	GAGTC	2 cut(s)	2097, 2327
MmeI	TCCRAC	6 cut(s)	312, 362, 372, 674, 714, 2340
Mox20I	TGGCCA	1 cut(s)	1425
MroI	TCCGGA	1 cut(s)	2062
MscI	TGGCCA	1 cut(s)	1425
MseI	TTAA	9 cut(s)	27, 780, 1113, 1575, 1628, 1679, 1884, 2367, 2572
MslI	CAYNNNNRTG	2 cut(s)	1128, 1956
Msp20I	TGGCCA	1 cut(s)	1425
MspA1I	CMGCKG	1 cut(s)	431
MspI	CCGG	7 cut(s)	253, 270, 291, 585, 753, 873, 2063
MunI	CAATTG	1 cut(s)	1029
Mva1269I	GAATGC	1 cut(s)	1731
MvaI	CCWGG	8 cut(s)	282, 447, 1344, 1512, 1603, 1797, 2190, 2283
MvnI	CGCG	1 cut(s)	669
MwoI	GCNNNNNNNGC	7 cut(s)	113, 145, 986, 1760, 1904, 2090, 2147
NciI	CCSGG	3 cut(s)	291, 586, 873
NcoI	CCATGG	1 cut(s)	1831
NdeI	CATATG	3 cut(s)	141, 457, 1279
NheI	GCTAGC	1 cut(s)	548
NlaIII	CATG	8 cut(s)	323, 353, 883, 1234, 1835, 2093, 2170, 2533
NmuCI	GTSAC	4 cut(s)	855, 1060, 1835, 2327
NspI	RCATGY	1 cut(s)	1234
NspV	TTCGAA	1 cut(s)	421
PasI	CCCWGGG	1 cut(s)	446
PceI	AGGCCT	1 cut(s)	729
PctI	GAATGC	1 cut(s)	1731
PfeI	GAWTC	9 cut(s)	179, 395, 1211, 1457, 1478, 1620, 1742, 2240, 2415
PfoI	TCCNGGA	1 cut(s)	584
PkrI	GCNGC	5 cut(s)	1439, 1762, 1948, 2374, 2428
Ple19I	CGATCG	1 cut(s)	2046
PleI	GAGTC	2 cut(s)	2097, 2326
PpsI	GAGTC	2 cut(s)	2097, 2326
PshAI	GACNNNNGTC	1 cut(s)	1148
PshBI	ATTAAT	1 cut(s)	27
PsiI	TTATAA	3 cut(s)	897, 1080, 1227
Psp124BI	GAGCTC	1 cut(s)	1303
Psp6I	CCWGG	8 cut(s)	280, 445, 1342, 1510, 1601, 1795, 2188, 2281
PspGI	CCWGG	8 cut(s)	280, 445, 1342, 1510, 1601, 1795, 2188, 2281
PspOMI	GGGCCC	1 cut(s)	285
PspPI	GGNCC	6 cut(s)	278, 285, 286, 1185, 1263, 1931
PsuI	RGATCY	3 cut(s)	334, 717, 1606
PvuI	CGATCG	1 cut(s)	2046
PvuII	CAGCTG	1 cut(s)	431
RsaI	GTAC	9 cut(s)	100, 251, 380, 797, 833, 1447, 1572, 1650, 2027
RsaNI	GTAC	9 cut(s)	99, 250, 379, 796, 832, 1446, 1571, 1649, 2026
RseI	CAYNNNNRTG	2 cut(s)	1128, 1956
SacI	GAGCTC	1 cut(s)	1303
SaqAI	TTAA	9 cut(s)	27, 780, 1113, 1575, 1628, 1679, 1884, 2367, 2572
SatI	GCNGC	5 cut(s)	1438, 1761, 1947, 2373, 2427
Sau96I	GGNCC	6 cut(s)	278, 285, 286, 1185, 1263, 1931
ScaI	AGTACT	1 cut(s)	797
SchI	GAGTC	2 cut(s)	2097, 2327
SduI	GDGCHC	5 cut(s)	150, 289, 1045, 1220, 1303
SfaNI	GCATC	7 cut(s)	451, 491, 853, 1792, 2137, 2215, 2503
SfuI	TTCGAA	1 cut(s)	421
SinI	GGWCC	3 cut(s)	278, 1263, 1931
SmiMI	CAYNNNNRTG	2 cut(s)	1128, 1956
SmlI	CTYRAG	2 cut(s)	302, 1850
SmoI	CTYRAG	2 cut(s)	302, 1850
SpeI	ACTAGT	1 cut(s)	488
SseBI	AGGCCT	1 cut(s)	729
SsiI	CCGC	3 cut(s)	499, 980, 1283
SstI	GAGCTC	1 cut(s)	1303
StuI	AGGCCT	1 cut(s)	729
StyI	CCWWGG	3 cut(s)	1831, 2313, 2485
TaaI	ACNGT	9 cut(s)	836, 1383, 1585, 1714, 1843, 2077, 2178, 2548, 2569
TaiI	ACGT	2 cut(s)	811, 1628
TaqI	TCGA	8 cut(s)	421, 485, 827, 861, 1774, 2042, 2046, 2306
TatI	WGTACW	3 cut(s)	98, 795, 831
TfiI	GAWTC	9 cut(s)	179, 395, 1211, 1457, 1478, 1620, 1742, 2240, 2415
Tru1I	TTAA	9 cut(s)	27, 780, 1113, 1575, 1628, 1679, 1884, 2367, 2572
Tru9I	TTAA	9 cut(s)	27, 780, 1113, 1575, 1628, 1679, 1884, 2367, 2572
TscAI	CASTG	5 cut(s)	249, 634, 1069, 1963, 2080
TseFI	GTSAC	4 cut(s)	855, 1060, 1835, 2327
TseI	GCWGC	5 cut(s)	1437, 1760, 1946, 2372, 2426
Tsp45I	GTSAC	4 cut(s)	855, 1060, 1835, 2327
TspDTI	ATGAA	8 cut(s)	23, 275, 896, 1071, 2078, 2456, 2518, 2550
TspGWI	ACGGA	1 cut(s)	1381
TspRI	CASTG	5 cut(s)	249, 634, 1069, 1963, 2080
VpaK11BI	GGWCC	3 cut(s)	278, 1263, 1931
VspI	ATTAAT	1 cut(s)	27
XapI	RAATTY	2 cut(s)	659, 2500
XbaI	TCTAGA	1 cut(s)	373
XceI	RCATGY	1 cut(s)	1234
XmiI	GTMKAC	1 cut(s)	743
ZrmI	AGTACT	1 cut(s)	797

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.