Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0004716

Physical Location & Seq

Genomic Coordinates Reverse (-)

2495879 .. 2502240

Gene Structure 6362 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1028500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2592 bp

ATGAAGGGTTTTGTGTTTTTCGATTTGTTAGCTGTGTTTGTAGTCTTTCTGCTATGTGGTTTTGCTGTGGGGAAGGAATGTACAAACAGTCCTACTCAGCTTTCATCGCATACTTACCGGTATGATCTCTTATCTTCCCACAATGAAACACTCAAGGAGGAGATGTTCTCACACTACCACTTGATACCTACCGATGATTCGGCGTGGATGGATTTGCTCCCAAGAAAGTTACTGAGGGATGAAGATGAAATTGAATGGGGAATGATGTACCGGAAAATGAAAGCTTTGGGTGGTTTTGAGGCATCCGGAGATTTTCTTAAGGAAGTGCCATTGCATGATGTGAGGTTGCATCCAACTTCACAACATGGAAGGGCACAGCAAACAAATTTGGAGTACTTATTGATGTTGGATTCAGATAGTTTAATTTGGAGCTTTCGTCAGACAGCGGGTTTGTCAACCCCCGGAACACCATACAAGGGGTGGGAGGACCCGACTTGTGAGCTTCGGGGACATTTCGTTGGGCATTACTTGAGTGCAACAGCACAAATGTGGGCTAGCACTCATAACCAGACCCTCAAAGATAAAATGTCTGCAATAGTGGACAATCTATCTCTTTGTCAGAAGAAAATTGGCAGTGGATATCTTTCTGCTTTTCCATCTGAAGAATTTGATCGATTTGAAGCTGTTAAGCCTGTCTGGGCTCCTTATTACACCATTCACAAGATATTGGCAGGCCTATTGGATCAGTATACATTTGCTGATAATGCTCAAGCATTGAAAATGGTGACGTGGATGGTTGAGTACTTTTACAACCGTGTTCAGAATGTGATATCGAAGTACAGTGTAGAAAGGCATTATACGGCACTTAACGAAGAAACTGGGGGGATGAATGATGTTCTCTATAAGCTATATGCTATTACGGGAGATGCAAAGCATTTATTGTTGGCTCATCTTTTCGACAAACCATGCTTTCTGGGAGTGTTGGCAGTGCAGGCTGAGGACATATCTGGTTTTCATGCTAACACACATATCCCAATTGTTATCGGTGCTCAAATGCGATATGAAGTCACTGGTGATCCACTTTATAAGGAAATGAGTTCTTTCTTTATGAACATTGTTAACTCTTCTCATAGCTATGCAACCGGAGGGACATCAGTCGGCGAGTTCTGGTCCGACCCTAAAAGGTTGGCAAGCACTCTAAAAACAGAGAACGAGGAGTCGTGCACAACTTATAACATGCTGAAGGTCTCTCGCCACCTGTTTAGATGGACCAAAGAAACAGCATATGCGGATTATTATGAGCGAGCTCTGACAAATGGCGTGCTAGGCATCCAAAGAGGGACAGAGCCTGGAGTCATGATTTACATGCTTCCACTAGGTCGTGGAACTTCCAAGGCCAGAAGTTACCATGGTTGGGGAAAACCGTTCGACTCTTTCTGGTGCTGCTATGGCACTGGAATTGAGTCATTCTCAAAGCTAGGAGATTCCATATATTTTGAAGAGGAGAAAGAAGCTCCTGCACTTTTCATTATCCAGTATATCTCAAGCTTTCTTGATTGGAAATCCGGACAAATTGCGCTTAATCAGACAGTTGATCCTGCCAATTCAAGGGATCCTTACCTTAGAGTCACGCTAACATTTTCTTCAGCGGAGGGGACAGGCATATCATCTACGTTGAACTTGCGAATTCCAATTTGGACAGATTCAACTGGTGCAAAAGCAGCATTGAATGGTCAAACTCTGAGTGTTCCAGCTCCAGGTAGTTTCCTAACAGTCACAAGAAAATGGAGTCCTGGTGACGTTTTGACGCTTCAGCTACCCTTTAGTCTAAGAACAGAGGCTATTAAAGATGATCGGCCTGAGTATGCTTCGATTCAAGCAATTCTTTATGGTCCATATCTGCTAGCGGCTCATACCAGTGGCAACTGGGACATTAAGACCGGCTCTGTTAGGTCTGTTTCTGACTGGATAACTCCAATCCCTTCCTCATACAATAACTTCTTGGTTTCTCTATCTCAAGAGTCTGGAAGCTCAAGTTTCGTCATAGCCAACTTAAACGAATCAATTGTACTGGACAAGTTTCCTGAAACCGGCACTGATGCGGCTAATCATGCCACTTTCAGGCTCGTTATAAATTACTTGTCCACAAAACTCTCTTCAGCAAATGATGTTAAAGGCAAATCGGTCATGCTAGAACTCTTTGATCTTCCAGGAAAGGTTGTAGTGCAGCAAGGGAAAGACAAGAACCTGGGTATAGCAGACATGTCCACTAGTAAAGACACTTCAGCTTTTCTTTTGGTTCCAGGTTTGGATGGAAAAAACACCACAGTATCCTTAGAGTCCGAAAGCAACGCAGGTTGCTATGTGTGTAGCGAAACAGTTAAGGCGAACGCAGGCTTGAAGCTCGTCTGCAGTTCAGAGTCATCGGGCGCCGAATTCAATCAGGCAGCCAGCTTTGTGATGAACAATGGAATAAATCAGTATCATCCTATCAGCTTTGTTGCTAAAGGGACACCAAGGAACTTTCTTCTAGCGCCATTGTCGACCTTCAAGGATGAATCTTATTCCGCTTATCTCAACATTTCGACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AbfB Glyco_hydro_127

Protein Analysis

864

Amino Acids

95.95

Weight (kDa)

5.74

Isoelectric Point (pI)

33.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-AFase-like_GH127_cat	PF07944	113 - 495	3.3e-113	Beta-L-arabinofuranosidase, GH127 catalytic domain
Glyco_hydro127M	PF20736	508 - 607	4.2e-14	Beta-L-arabinofuranosidase, GH127 middle domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004716)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12950 AT5G12960
fragaria_vesca	FvH4_3g43250 FvH4_3g43250 FvH4_3g43250
malus_domestica	MD03G1023400.v1.1 MD11G1028500.v1.1
prunus_persica	Prupe.6G022600_v2.0.a1 Prupe.6G022600_v2.0.a1 Prupe.6G022600_v2.0.a1 Prupe.6G022600_v2.0.a1 Prupe.6G022600_v2.0.a1
pyrus_communis	pycom03g02000 pycom11g02200
rosa_chinensis	RchiOBHm_Chr5g0077301 RchiOBHm_Chr5g0077311
rosa_laevigata	RLG00000002632 RLG00000036683 RLG00000036684
rosa_multiflora	Rmu_sc0000433.1_g000041
rosa_roxburghii	Rroxscaffold_1G00004600 Rroxscaffold_1G00004670
rosa_rugosa	Rorug05G0453600
rosa_samantha	Rh5AG508600 Rh5AG508700 Rh5BG529700 Rh5BG529800 Rh5DG542300 Rh5DG542400
rosa_wichuraiana	Rw5G046870 Rw5G046880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1086, 1233, 2132
Acc36I	ACCTGC	1 cut(s)	2345
AccB1I	GGYRCC	1 cut(s)	2429
AccI	GTMKAC	2 cut(s)	749, 2543
AccIII	TCCGGA	2 cut(s)	305, 1565
AciI	CCGC	6 cut(s)	446, 1289, 1649, 1907, 2102, 2568
AclWI	GGATC	5 cut(s)	750, 1070, 1589, 1607, 1620
AcsI	RAATTY	4 cut(s)	385, 665, 1686, 2435
AcuI	CTGAAG	6 cut(s)	681, 1262, 1629, 1796, 2142, 2268
AcyI	GRCGYC	1 cut(s)	2430
AfaI	GTAC	6 cut(s)	82, 269, 395, 803, 839, 2070
AfiI	CCNNNNNNNGG	6 cut(s)	476, 1414, 1608, 1757, 2090, 2121
AflII	CTTAAG	1 cut(s)	317
AflIII	ACRYGT	1 cut(s)	2262
AgeI	ACCGGT	1 cut(s)	117
AhlI	ACTAGT	1 cut(s)	2270
AjiI	CACGTC	1 cut(s)	789
AjnI	CCWGG	6 cut(s)	1348, 1756, 1792, 2209, 2247, 2302
AjuI	GAANNNNNNNTTGG	2 cut(s)	1678, 1710
AleI	CACNNNNGTG	1 cut(s)	547
AloI	GAACNNNNNNTCC	2 cut(s)	149, 181
Alw21I	GWGCWC	3 cut(s)	1051, 1226, 1309
Alw26I	GTCTC	1 cut(s)	1252
Alw44I	GTGCAC	1 cut(s)	1222
AlwI	GGATC	5 cut(s)	750, 1070, 1589, 1607, 1620
AlwNI	CAGNNNCTG	1 cut(s)	1349
Aor13HI	TCCGGA	2 cut(s)	305, 1565
AoxI	GGCC	3 cut(s)	733, 1395, 1856
ApaLI	GTGCAC	1 cut(s)	1222
ApeKI	GCWGC	4 cut(s)	1443, 1721, 2227, 2447
ApoI	RAATTY	4 cut(s)	385, 665, 1686, 2435
ArsI	GACNNNNNNTTYG	2 cut(s)	433, 465
AsiGI	ACCGGT	1 cut(s)	117
AspLEI	GCGC	3 cut(s)	1579, 2432, 2536
AspS9I	GGNCC	4 cut(s)	487, 1170, 1269, 1892
AsuC2I	CCSGG	1 cut(s)	462
AsuHPI	GGTGA	3 cut(s)	796, 1085, 1808
AsuNHI	GCTAGC	2 cut(s)	554, 1903
AvaII	GGWCC	4 cut(s)	487, 1170, 1269, 1892
BaeGI	GKGCMC	2 cut(s)	376, 1226
BaeI	ACNNNNGTAYC	2 cut(s)	2313, 2346
BamHI	GGATCC	1 cut(s)	1612
BanI	GGYRCC	1 cut(s)	2429
BanII	GRGCYC	2 cut(s)	703, 1309
BarI	GAAGNNNNNNTAC	2 cut(s)	2266, 2298
Bbv12I	GWGCWC	3 cut(s)	1051, 1226, 1309
BbvCI	CCTCAGC	1 cut(s)	996
BbvI	GCAGC	4 cut(s)	1430, 1733, 2239, 2459
BccI	CCATC	5 cut(s)	202, 664, 787, 1260, 2306
BceAI	ACGGC	1 cut(s)	876
BciT130I	CCWGG	6 cut(s)	1350, 1758, 1794, 2211, 2249, 2304
BciVI	GTATCC	1 cut(s)	2341
BcnI	CCSGG	1 cut(s)	462
BcoDI	GTCTC	1 cut(s)	1252
BcuI	ACTAGT	1 cut(s)	2270
BfaI	CTAG	8 cut(s)	555, 1325, 1376, 1478, 1904, 2192, 2271, 2531
BfmI	CTRYAG	1 cut(s)	2410
BfoI	RGCGCY	2 cut(s)	2433, 2537
BfrI	CTTAAG	1 cut(s)	317
BfuAI	ACCTGC	1 cut(s)	2345
BfuI	GTATCC	1 cut(s)	2341
BisI	GCNGC	6 cut(s)	1444, 1722, 1908, 2103, 2228, 2448
BlsI	GCNGC	6 cut(s)	1445, 1723, 1909, 2104, 2229, 2449
BmcAI	AGTACT	2 cut(s)	395, 803
Bme1390I	CCNGG	7 cut(s)	462, 1350, 1758, 1794, 2211, 2249, 2304
Bme18I	GGWCC	4 cut(s)	487, 1170, 1269, 1892
BmgBI	CACGTC	1 cut(s)	789
BmgT120I	GGNCC	4 cut(s)	487, 1170, 1269, 1892
BmiI	GGNNCC	5 cut(s)	489, 702, 1614, 2301, 2431
BmrFI	CCNGG	7 cut(s)	462, 1350, 1758, 1794, 2211, 2249, 2304
BmrI	ACTGGG	2 cut(s)	888, 1936
BmsI	GCATC	5 cut(s)	311, 358, 916, 1338, 2089
BmtI	GCTAGC	2 cut(s)	558, 1907
BmuI	ACTGGG	2 cut(s)	888, 1936
BoxI	GACNNNNGTC	1 cut(s)	1154
BplI	GAGNNNNNCTC	4 cut(s)	152, 184, 1454, 1486
BpmI	CTGGAG	2 cut(s)	1371, 1740
Bpu10I	CCTNAGC	1 cut(s)	996
BpuEI	CTTGAG	6 cut(s)	137, 550, 753, 1528, 2001, 2017
BpuMI	CCSGG	1 cut(s)	462
Bsa29I	ATCGAT	1 cut(s)	673
BsaHI	GRCGYC	1 cut(s)	2430
BsaI	GGTCTC	1 cut(s)	1252
BsaJI	CCNNGG	5 cut(s)	460, 1392, 1408, 2248, 2516
BsaWI	WCCGGW	5 cut(s)	117, 270, 305, 1142, 1565
BsaXI	ACNNNNNCTCC	6 cut(s)	149, 179, 421, 451, 1780, 1810
Bsc4I	CCNNNNNNNGG	6 cut(s)	476, 1414, 1608, 1757, 2090, 2121
Bse118I	RCCGGY	3 cut(s)	117, 1940, 2090
Bse1I	ACTGG	9 cut(s)	883, 1075, 1459, 1534, 1714, 1917, 1931, 1970, 2076
Bse3DI	GCAATG	1 cut(s)	329
BseAI	TCCGGA	2 cut(s)	305, 1565
BseBI	CCWGG	6 cut(s)	1350, 1758, 1794, 2211, 2249, 2304
BseCI	ATCGAT	1 cut(s)	673
BseDI	CCNNGG	5 cut(s)	460, 1392, 1408, 2248, 2516
BseLI	CCNNNNNNNGG	6 cut(s)	476, 1414, 1608, 1757, 2090, 2121
BseMI	GCAATG	1 cut(s)	329
BseMII	CTCAG	5 cut(s)	110, 224, 987, 1733, 1851
BseNI	ACTGG	9 cut(s)	883, 1075, 1459, 1534, 1714, 1917, 1931, 1970, 2076
BseRI	GAGGAG	3 cut(s)	173, 1229, 1517
BseSI	GKGCMC	2 cut(s)	376, 1226
BseXI	GCAGC	4 cut(s)	1430, 1733, 2239, 2459
BsgI	GTGCAG	3 cut(s)	1010, 1503, 2246
BshFI	GGCC	3 cut(s)	735, 1397, 1858
BshNI	GGYRCC	1 cut(s)	2429
BshTI	ACCGGT	1 cut(s)	117
BshVI	ATCGAT	1 cut(s)	673
BsiHKAI	GWGCWC	3 cut(s)	1051, 1226, 1309
BsiSI	CCGG	8 cut(s)	118, 271, 306, 462, 1143, 1566, 1941, 2091
BslFI	GGGAC	6 cut(s)	522, 1162, 1354, 1669, 1943, 2524
BslI	CCNNNNNNNGG	6 cut(s)	476, 1414, 1608, 1757, 2090, 2121
BsmAI	GTCTC	1 cut(s)	1252
BsmFI	GGGAC	6 cut(s)	522, 1162, 1354, 1669, 1943, 2524
BsnI	GGCC	3 cut(s)	735, 1397, 1858
Bso31I	GGTCTC	1 cut(s)	1252
Bsp1286I	GDGCHC	5 cut(s)	376, 703, 1051, 1226, 1309
Bsp13I	TCCGGA	2 cut(s)	305, 1565
Bsp1407I	TGTACA	1 cut(s)	80
Bsp143I	GATC	8 cut(s)	124, 670, 742, 1075, 1594, 1612, 1852, 2203
Bsp19I	CCATGG	1 cut(s)	1408
BspACI	CCGC	6 cut(s)	446, 1289, 1649, 1907, 2102, 2568
BspANI	GGCC	3 cut(s)	735, 1397, 1858
BspCNI	CTCAG	5 cut(s)	109, 225, 988, 1734, 1852
BspDI	ATCGAT	1 cut(s)	673
BspEI	TCCGGA	2 cut(s)	305, 1565
BspHI	TCATGA	1 cut(s)	1356
BspLI	GGNNCC	5 cut(s)	489, 702, 1614, 2301, 2431
BspMAI	CTGCAG	1 cut(s)	2414
BspMI	ACCTGC	1 cut(s)	2345
BspOI	GCTAGC	2 cut(s)	558, 1907
BspPI	GGATC	5 cut(s)	750, 1070, 1589, 1607, 1620
BspT107I	GGYRCC	1 cut(s)	2429
BspTI	CTTAAG	1 cut(s)	317
BspTNI	GGTCTC	1 cut(s)	1252
BsrDI	GCAATG	1 cut(s)	329
BsrFI	RCCGGY	3 cut(s)	117, 1940, 2090
BsrGI	TGTACA	1 cut(s)	80
BsrI	ACTGG	9 cut(s)	883, 1075, 1459, 1534, 1714, 1917, 1931, 1970, 2076
BssAI	RCCGGY	3 cut(s)	117, 1940, 2090
BssECI	CCNNGG	5 cut(s)	460, 1392, 1408, 2248, 2516
BssMI	GATC	8 cut(s)	124, 670, 742, 1075, 1594, 1612, 1852, 2203
BssNAI	GTATAC	1 cut(s)	750
BssNI	GRCGYC	1 cut(s)	2430
BssT1I	CCWWGG	3 cut(s)	1392, 1408, 2516
Bst1107I	GTATAC	1 cut(s)	750
Bst2UI	CCWGG	6 cut(s)	1350, 1758, 1794, 2211, 2249, 2304
Bst4CI	ACNGT	8 cut(s)	89, 815, 842, 1425, 1591, 1774, 2329, 2380
Bst6I	CTCTTC	3 cut(s)	1129, 1494, 2161
BstACI	GRCGYC	1 cut(s)	2430
BstAFI	CTTAAG	1 cut(s)	317
BstAUI	TGTACA	1 cut(s)	80
BstC8I	GCNNGC	9 cut(s)	556, 733, 993, 1192, 1305, 1322, 1905, 2395, 2452
BstDEI	CTNAG	8 cut(s)	96, 233, 996, 1622, 1742, 1829, 1860, 2335
BstDSI	CCRYGG	1 cut(s)	1408
BstH2I	RGCGCY	2 cut(s)	2433, 2537
BstHHI	GCGC	3 cut(s)	1579, 2432, 2536
BstKTI	GATC	8 cut(s)	127, 673, 745, 1078, 1597, 1615, 1855, 2206
BstMAI	GTCTC	1 cut(s)	1252
BstMBI	GATC	8 cut(s)	124, 670, 742, 1075, 1594, 1612, 1852, 2203
BstMWI	GCNNNNNNNGC	7 cut(s)	106, 764, 992, 1326, 1449, 1721, 2111
BstNI	CCWGG	6 cut(s)	1350, 1758, 1794, 2211, 2249, 2304
BstNSI	RCATGY	3 cut(s)	1240, 1369, 2266
BstPAI	GACNNNNGTC	1 cut(s)	1154
BstSCI	CCNGG	7 cut(s)	460, 1348, 1756, 1792, 2209, 2247, 2302
BstSFI	CTRYAG	1 cut(s)	2410
BstSLI	GKGCMC	2 cut(s)	376, 1226
BstV1I	GCAGC	4 cut(s)	1430, 1733, 2239, 2459
BstX2I	RGATCY	1 cut(s)	1612
BstYI	RGATCY	1 cut(s)	1612
BstZ17I	GTATAC	1 cut(s)	750
Bsu15I	ATCGAT	1 cut(s)	673
BsuI	GTATCC	1 cut(s)	2341
BsuRI	GGCC	3 cut(s)	735, 1397, 1858
BsuTUI	ATCGAT	1 cut(s)	673
BtgI	CCRYGG	1 cut(s)	1408
BtgZI	GCGATG	1 cut(s)	90
BtrI	CACGTC	1 cut(s)	789
BtsI	GCAGTG	2 cut(s)	640, 993
BtsIMutI	CAGTG	7 cut(s)	640, 847, 993, 1068, 1452, 1924, 2094
BveI	ACCTGC	1 cut(s)	2345
Cac8I	GCNNGC	9 cut(s)	556, 733, 993, 1192, 1305, 1322, 1905, 2395, 2452
CaiI	CAGNNNCTG	1 cut(s)	1349
CciI	TCATGA	1 cut(s)	1356
CfoI	GCGC	3 cut(s)	1579, 2432, 2536
Cfr10I	RCCGGY	3 cut(s)	117, 1940, 2090
Cfr13I	GGNCC	4 cut(s)	487, 1170, 1269, 1892
ClaI	ATCGAT	1 cut(s)	673
CseI	GACGC	1 cut(s)	1816
Csp6I	GTAC	6 cut(s)	81, 268, 394, 802, 838, 2069
CspAI	ACCGGT	1 cut(s)	117
CviQI	GTAC	6 cut(s)	81, 268, 394, 802, 838, 2069
DdeI	CTNAG	8 cut(s)	96, 233, 996, 1622, 1742, 1829, 1860, 2335
DinI	GGCGCC	1 cut(s)	2431
DpnI	GATC	8 cut(s)	126, 672, 744, 1077, 1596, 1614, 1854, 2205
DpnII	GATC	8 cut(s)	124, 670, 742, 1075, 1594, 1612, 1852, 2203
Eam1104I	CTCTTC	3 cut(s)	1129, 1494, 2161
EarI	CTCTTC	3 cut(s)	1129, 1494, 2161
Ecl136II	GAGCTC	1 cut(s)	1307
Eco130I	CCWWGG	3 cut(s)	1392, 1408, 2516
Eco147I	AGGCCT	1 cut(s)	735
Eco24I	GRGCYC	2 cut(s)	703, 1309
Eco31I	GGTCTC	1 cut(s)	1252
Eco32I	GATATC	2 cut(s)	641, 831
Eco47I	GGWCC	4 cut(s)	487, 1170, 1269, 1892
Eco53kI	GAGCTC	1 cut(s)	1307
Eco57I	CTGAAG	6 cut(s)	681, 1262, 1629, 1796, 2142, 2268
EcoICRI	GAGCTC	1 cut(s)	1307
EcoO109I	RGGNCCY	1 cut(s)	487
EcoRI	GAATTC	2 cut(s)	1686, 2435
EcoRII	CCWGG	6 cut(s)	1348, 1756, 1792, 2209, 2247, 2302
EcoRV	GATATC	2 cut(s)	641, 831
EcoT14I	CCWWGG	3 cut(s)	1392, 1408, 2516
EcoT38I	GRGCYC	2 cut(s)	703, 1309
EgeI	GGCGCC	1 cut(s)	2431
EheI	GGCGCC	1 cut(s)	2431
ErhI	CCWWGG	3 cut(s)	1392, 1408, 2516
FalI	AAGNNNNNCTT	4 cut(s)	1600, 1632, 1869, 1901
FaqI	GGGAC	6 cut(s)	522, 1162, 1354, 1669, 1943, 2524
FauI	CCCGC	1 cut(s)	439
FauNDI	CATATG	1 cut(s)	1285
FblI	GTMKAC	2 cut(s)	749, 2543
Fnu4HI	GCNGC	6 cut(s)	1444, 1722, 1908, 2103, 2228, 2448
FriOI	GRGCYC	2 cut(s)	703, 1309
Fsp4HI	GCNGC	6 cut(s)	1444, 1722, 1908, 2103, 2228, 2448
FspBI	CTAG	8 cut(s)	555, 1325, 1376, 1478, 1904, 2192, 2271, 2531
GlaI	GCGC	3 cut(s)	1578, 2431, 2535
GluI	GCNGC	6 cut(s)	1444, 1722, 1908, 2103, 2228, 2448
GsuI	CTGGAG	2 cut(s)	1371, 1740
HaeII	RGCGCY	2 cut(s)	2433, 2537
HaeIII	GGCC	3 cut(s)	735, 1397, 1858
HapII	CCGG	8 cut(s)	118, 271, 306, 462, 1143, 1566, 1941, 2091
HgaI	GACGC	1 cut(s)	1816
HhaI	GCGC	3 cut(s)	1579, 2432, 2536
Hin1I	GRCGYC	1 cut(s)	2430
Hin6I	GCGC	3 cut(s)	1577, 2430, 2534
HinP1I	GCGC	3 cut(s)	1577, 2430, 2534
HincII	GTYRAC	3 cut(s)	456, 1120, 2544
HindII	GTYRAC	3 cut(s)	456, 1120, 2544
HindIII	AAGCTT	2 cut(s)	282, 1546
HpaI	GTTAAC	1 cut(s)	1120
HpaII	CCGG	8 cut(s)	118, 271, 306, 462, 1143, 1566, 1941, 2091
HphI	GGTGA	3 cut(s)	796, 1085, 1808
Hpy166II	GTNNAC	8 cut(s)	456, 601, 750, 1120, 1224, 2145, 2268, 2544
Hpy188III	TCNNGA	7 cut(s)	306, 1357, 1553, 1566, 2018, 2025, 2084
Hpy8I	GTNNAC	8 cut(s)	456, 601, 750, 1120, 1224, 2145, 2268, 2544
HpyAV	CCTTC	5 cut(s)	67, 363, 1237, 1992, 2557
HpyCH4III	ACNGT	8 cut(s)	89, 815, 842, 1425, 1591, 1774, 2329, 2380
HpyCH4IV	ACGT	3 cut(s)	788, 1673, 1800
HpyF10VI	GCNNNNNNNGC	7 cut(s)	106, 764, 992, 1326, 1449, 1721, 2111
HpyF3I	CTNAG	8 cut(s)	96, 233, 996, 1622, 1742, 1829, 1860, 2335
HpySE526I	ACGT	3 cut(s)	788, 1673, 1800
Hsp92I	GRCGYC	1 cut(s)	2430
HspAI	GCGC	3 cut(s)	1577, 2430, 2534
KasI	GGCGCC	1 cut(s)	2429
Kpn2I	TCCGGA	2 cut(s)	305, 1565
KspAI	GTTAAC	1 cut(s)	1120
Kzo9I	GATC	8 cut(s)	124, 670, 742, 1075, 1594, 1612, 1852, 2203
LmnI	GCTCC	5 cut(s)	222, 429, 706, 1519, 1759
Lsp1109I	GCAGC	4 cut(s)	1430, 1733, 2239, 2459
LweI	GCATC	5 cut(s)	311, 358, 916, 1338, 2089
MaeI	CTAG	8 cut(s)	555, 1325, 1376, 1478, 1904, 2192, 2271, 2531
MaeII	ACGT	3 cut(s)	788, 1673, 1800
MaeIII	GTNAC	7 cut(s)	228, 784, 1066, 1403, 1627, 1774, 1796
MalI	GATC	8 cut(s)	126, 672, 744, 1077, 1596, 1614, 1854, 2205
MboI	GATC	8 cut(s)	124, 670, 742, 1075, 1594, 1612, 1852, 2203
MfeI	CAATTG	2 cut(s)	1035, 2064
MflI	RGATCY	1 cut(s)	1612
MhlI	GDGCHC	5 cut(s)	376, 703, 1051, 1226, 1309
Mly113I	GGCGCC	1 cut(s)	2430
MlyI	GAGTC	9 cut(s)	1226, 1362, 1424, 1472, 1635, 1798, 2030, 2348, 2429
MmeI	TCCRAC	3 cut(s)	377, 387, 1197
MroI	TCCGGA	2 cut(s)	305, 1565
MslI	CAYNNNNRTG	3 cut(s)	547, 1134, 1917
MspA1I	CMGCKG	2 cut(s)	446, 1649
MspCI	CTTAAG	1 cut(s)	317
MspI	CCGG	8 cut(s)	118, 271, 306, 462, 1143, 1566, 1941, 2091
MspR9I	CCNGG	7 cut(s)	462, 1350, 1758, 1794, 2211, 2249, 2304
MunI	CAATTG	2 cut(s)	1035, 2064
MvaI	CCWGG	6 cut(s)	1350, 1758, 1794, 2211, 2249, 2304
MwoI	GCNNNNNNNGC	7 cut(s)	106, 764, 992, 1326, 1449, 1721, 2111
NarI	GGCGCC	1 cut(s)	2430
NciI	CCSGG	1 cut(s)	462
NcoI	CCATGG	1 cut(s)	1408
NdeI	CATATG	1 cut(s)	1285
NdeII	GATC	8 cut(s)	124, 670, 742, 1075, 1594, 1612, 1852, 2203
NheI	GCTAGC	2 cut(s)	554, 1903
NlaIV	GGNNCC	5 cut(s)	489, 702, 1614, 2301, 2431
NmuCI	GTSAC	5 cut(s)	784, 1066, 1627, 1774, 1796
NspI	RCATGY	3 cut(s)	1240, 1369, 2266
OliI	CACNNNNGTG	1 cut(s)	547
PagI	TCATGA	1 cut(s)	1356
PceI	AGGCCT	1 cut(s)	735
PciI	ACATGT	1 cut(s)	2262
PfeI	GAWTC	7 cut(s)	197, 410, 1484, 1703, 1873, 2060, 2558
PfoI	TCCNGGA	1 cut(s)	2209
PinAI	ACCGGT	1 cut(s)	117
PkrI	GCNGC	6 cut(s)	1445, 1723, 1909, 2104, 2229, 2449
PleI	GAGTC	9 cut(s)	1225, 1361, 1424, 1471, 1634, 1797, 2029, 2347, 2428
PluTI	GGCGCC	1 cut(s)	2433
PpsI	GAGTC	9 cut(s)	1225, 1361, 1424, 1471, 1634, 1797, 2029, 2347, 2428
PpuMI	RGGWCCY	1 cut(s)	487
PscI	ACATGT	1 cut(s)	2262
PshAI	GACNNNNGTC	1 cut(s)	1154
PsiI	TTATAA	3 cut(s)	1086, 1233, 2132
Psp124BI	GAGCTC	1 cut(s)	1309
Psp5II	RGGWCCY	1 cut(s)	487
Psp6I	CCWGG	6 cut(s)	1348, 1756, 1792, 2209, 2247, 2302
PspGI	CCWGG	6 cut(s)	1348, 1756, 1792, 2209, 2247, 2302
PspN4I	GGNNCC	5 cut(s)	489, 702, 1614, 2301, 2431
PspPI	GGNCC	4 cut(s)	487, 1170, 1269, 1892
PspPPI	RGGWCCY	1 cut(s)	487
PstI	CTGCAG	1 cut(s)	2414
PstNI	CAGNNNCTG	1 cut(s)	1349
PsuI	RGATCY	1 cut(s)	1612
RsaI	GTAC	6 cut(s)	82, 269, 395, 803, 839, 2070
RsaNI	GTAC	6 cut(s)	81, 268, 394, 802, 838, 2069
RseI	CAYNNNNRTG	3 cut(s)	547, 1134, 1917
SacI	GAGCTC	1 cut(s)	1309
SalI	GTCGAC	1 cut(s)	2542
SatI	GCNGC	6 cut(s)	1444, 1722, 1908, 2103, 2228, 2448
Sau3AI	GATC	8 cut(s)	124, 670, 742, 1075, 1594, 1612, 1852, 2203
Sau96I	GGNCC	4 cut(s)	487, 1170, 1269, 1892
ScaI	AGTACT	2 cut(s)	395, 803
SchI	GAGTC	9 cut(s)	1226, 1362, 1424, 1472, 1635, 1798, 2030, 2348, 2429
ScrFI	CCNGG	7 cut(s)	462, 1350, 1758, 1794, 2211, 2249, 2304
SduI	GDGCHC	5 cut(s)	376, 703, 1051, 1226, 1309
SfaNI	GCATC	5 cut(s)	311, 358, 916, 1338, 2089
SfcI	CTRYAG	1 cut(s)	2410
SfoI	GGCGCC	1 cut(s)	2431
SinI	GGWCC	4 cut(s)	487, 1170, 1269, 1892
SmiMI	CAYNNNNRTG	3 cut(s)	547, 1134, 1917
SmlI	CTYRAG	7 cut(s)	152, 317, 529, 768, 1543, 2016, 2032
SmoI	CTYRAG	7 cut(s)	152, 317, 529, 768, 1543, 2016, 2032
SpeI	ACTAGT	1 cut(s)	2270
SseBI	AGGCCT	1 cut(s)	735
SsiI	CCGC	6 cut(s)	446, 1289, 1649, 1907, 2102, 2568
SspDI	GGCGCC	1 cut(s)	2429
SspMI	CTAG	8 cut(s)	555, 1325, 1376, 1478, 1904, 2192, 2271, 2531
SstI	GAGCTC	1 cut(s)	1309
StuI	AGGCCT	1 cut(s)	735
StyD4I	CCNGG	7 cut(s)	460, 1348, 1756, 1792, 2209, 2247, 2302
StyI	CCWWGG	3 cut(s)	1392, 1408, 2516
TaaI	ACNGT	8 cut(s)	89, 815, 842, 1425, 1591, 1774, 2329, 2380
TaiI	ACGT	3 cut(s)	791, 1676, 1803
TaqI	TCGA	8 cut(s)	21, 673, 833, 957, 1428, 1871, 2543, 2585
TaqII	GACCGA	1 cut(s)	2173
TatI	WGTACW	5 cut(s)	80, 393, 801, 837, 2068
TauI	GCSGC	2 cut(s)	1910, 2105
TfiI	GAWTC	7 cut(s)	197, 410, 1484, 1703, 1873, 2060, 2558
TscAI	CASTG	7 cut(s)	640, 847, 993, 1075, 1459, 1924, 2101
TseFI	GTSAC	5 cut(s)	784, 1066, 1627, 1774, 1796
TseI	GCWGC	4 cut(s)	1443, 1721, 2227, 2447
Tsp45I	GTSAC	5 cut(s)	784, 1066, 1627, 1774, 1796
TspRI	CASTG	7 cut(s)	640, 847, 993, 1075, 1459, 1924, 2101
Vha464I	CTTAAG	1 cut(s)	317
VneI	GTGCAC	1 cut(s)	1222
VpaK11BI	GGWCC	4 cut(s)	487, 1170, 1269, 1892
XapI	RAATTY	4 cut(s)	385, 665, 1686, 2435
XceI	RCATGY	3 cut(s)	1240, 1369, 2266
XcmI	CCANNNNNNNNNTGG	1 cut(s)	477
XmiI	GTMKAC	2 cut(s)	749, 2543
XspI	CTAG	8 cut(s)	555, 1325, 1376, 1478, 1904, 2192, 2271, 2531
ZrmI	AGTACT	2 cut(s)	395, 803

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.