Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5D

Orthogroup ID

OG0004716

Physical Location & Seq

Genomic Coordinates Forward (+)

83462962 .. 83468173

Gene Structure 5212 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5DG542400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2445 bp

ATGGTGCTCTCACACTATCACTTGACACCAAGTGATGATTCTGCTCGGTTGAAGTTGCACCCAAGAAAGATGTTGAGGGAGGAAGATGGATATGAGTGGTCAGTGATGTACCGGAAGATGAAAACCTCCGGTGGTGGTCCTGGGGCCCCTGGAGGTCTTCTCAAGGAAGTGTCATTGCATGATGTGAGGTTGGATCCAACTTCACAACATGGGAAGGCACAGCAAACAAATCTAGAGTACCTGTTGATGTTGGATTCGGATAATTTACTTTGGAACTTTCGAAAGACAGCTGGTTTAGAAACCCCTGGGGAACCATATGATGCTACAAGTTCGTGGGAGGCGTCAACTAGTGAACTGCGGGGTCATTTTGTAGGGCATTATTTGAGTGCTTCAGCAATGATGTGGGCCAGCACTCATAATGATACTCTCAAAGAGAAGATGTCCGGGATTGTGTCGAGTCTTTCTCTTTGTCAGAAGGAAATTGGCAGTGGATATCTTTCGGCTTTTCCAACTGAGGAATTTGATCGCGTTGAAGCTCATACACCTGTCTGGGTTCCTTATTATACCATTCACAAGATCTTGGCAGGCCTGTTGGATCAGTATACACTTGCTGGTAGTGATCAAGCTTTTAGTATGATGAAATGGATGGTTGAGTACTTTTACAAACGTGTTCAGAATGTGATATCGAAGTACAGTATAGCAAAACATTATCTGTCACTCGATGCAGAACCCGGTGGCATGAATGACATTCTTTATAAATTATATGCTATCACGGGAGATACAAAGCATTTATTGTTGGCTCATCTTTTTGACAAACCCTGCTTTCTAGGATTGTTGGCGGTGCAGGCTGATGACATATCTTATTTTCACGCTAACACGCATATTGCAATTGTCATTGGTGCTCAAAAGCGCTATGAAGTCACTGGTGATCCACTTTATAAGGAAATGGGGATTTTCTTTATGGACATTGTTAACTCTTCTCACAGCTATGCAACAGGAGGGACATCAGTCGGCGAGCATTGGCAGGAACCTAAAAGATTGGGGACCTATCTACGATCAGACAATGAGGAATCGTGCACAACTTATAGCATGCTGAAGGTCTCTCGCAACCTGTTTAGATGGACCAAAGAAACTGCGTATGCGGATTATTATGAGCGAGCTCTAACAAATGGTGTTCTAGGTATCCAAAGAGGAACTGAGCCTGGAGTTATGATCTACTTCCTCCCACTAGGCCGTGGACAGTCCAAGTCCGTGAGTTATTTTGGTTGGGGATCACCATTTGGCCATTTCTGGTGCTGCTATGGTACTGGGATTGAATCATTCTCAAAGTTAGGAGATTCCATATATTTTGAAGAGGAAGGGGAAACTCCTGGTCTTTACATTATCCAGTATATATCAAGCTTGCTTGATTGGAAATCTGGGCAAGTCGTACTTAATCAGACAGTTGGTAACATCAATTCCTGGGATCCTTACCTTAGAATCACGTTAACATTTTCTTCCACGAAGGGGACAGGCAAATCATCTACCGTGAACTTGCGAATGCCAATTTGGACAGATTCAGATGGTGCAAAGGCAGCATTGAATGGTCGAAATCTGCATCCATCAGCTCCAGGTAGTTTTCTACCAATCCCAAATAAATGGAGCCATGGTGACACAGTAACTCTTGAACTACCCATTAGTCTGAGAACAGAACCTATTAAAGATGACCGGGCAGAGTATGCTTCAGTCCAAGCAATTCTTTATGGTCCAGTTCTACTTGCGGCTCATACCAGTGGTAACTGGGACATCCAAACTAAGTCAACTAATCTTTCAGACTGGATAACTCCACTCCCTTTTTCGCACAATAATTATTTGGTTTCTTTCTCCCAAGAGTTCGAAAACTCAACTTTTGCCTTGGCCAATTTAAACCAGTCGATCGAACTAGACAACTTTCCGGATGTAGGCACTGTTGCTGCTGTTCATGCCACTTTCAGACTCATTTTAGATGATTTATCAACAAAAGATTTCAAAGCACAAGATGCTATTGGAAAATCAGTCATGCTAGAACTGTTTGATCTTCCTGGGAAGGTTCTTGGGCATCAAAAGAAAGACAAGAACCTTGTGATAGCAGATTCTTCAAGTAGCAAGGAGAACATAGTTTTTCGCTTGGTTCCAGGTTTGGATGGAAAGTCTGCCTCGGTCTCCTTGGAGTCAGAAAGTGACAAGGGTTGCTTTGTGTATAGCTCGGAGTTGGAGGTTAAAGCAGCCTTGAAGCTCGGTTGCAGTTCAAAATCGTCAAACGATGGATTCAAACAGGCAGCCAGCTTTGTAATGAAGAGTGGAATAAGTCAGTATCATCCTATCAGCTTTGTTGCCCAAGGGAAGCATCGGAATTTTCTTCTACAACCATTGTCAAGCTTCATGGATGAAACTTATACCGTCTATCTCAACATTACAACAGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AbfB Glyco_hydro_127

Protein Analysis

814

Amino Acids

90.64

Weight (kDa)

5.96

Isoelectric Point (pI)

34.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-AFase-like_GH127_cat	PF07944	61 - 446	9.8e-105	Beta-L-arabinofuranosidase, GH127 catalytic domain
Glyco_hydro127M	PF20736	459 - 559	1.5e-13	Beta-L-arabinofuranosidase, GH127 middle domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004716)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12950 AT5G12960
fragaria_vesca	FvH4_3g43250 FvH4_3g43250 FvH4_3g43250
malus_domestica	MD03G1023400.v1.1 MD11G1028500.v1.1
prunus_persica	Prupe.6G022600_v2.0.a1 Prupe.6G022600_v2.0.a1 Prupe.6G022600_v2.0.a1 Prupe.6G022600_v2.0.a1 Prupe.6G022600_v2.0.a1
pyrus_communis	pycom03g02000 pycom11g02200
rosa_chinensis	RchiOBHm_Chr5g0077301 RchiOBHm_Chr5g0077311
rosa_laevigata	RLG00000002632 RLG00000036683 RLG00000036684
rosa_multiflora	Rmu_sc0000433.1_g000041
rosa_roxburghii	Rroxscaffold_1G00004600 Rroxscaffold_1G00004670
rosa_rugosa	Rorug05G0453600
rosa_samantha	Rh5AG508600 Rh5AG508700 Rh5BG529700 Rh5BG529800 Rh5DG542300 Rh5DG542400
rosa_wichuraiana	Rw5G046870 Rw5G046880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	756, 939
AccI	GTMKAC	1 cut(s)	602
AccII	CGCG	1 cut(s)	528
AccIII	TCCGGA	1 cut(s)	1933
AciI	CCGC	4 cut(s)	358, 839, 1142, 1760
AclWI	GGATC	7 cut(s)	188, 201, 603, 923, 1279, 1460, 1473
AcoI	YGGCCR	2 cut(s)	1282, 1896
AcsI	RAATTY	2 cut(s)	518, 2371
AcuI	CTGAAG	3 cut(s)	375, 1115, 1707
AcyI	GRCGYC	1 cut(s)	341
AdeI	CACNNNGTG	1 cut(s)	32
AfaI	GTAC	6 cut(s)	110, 239, 656, 692, 1306, 1431
AfeI	AGCGCT	1 cut(s)	911
AfiI	CCNNNNNNNGG	2 cut(s)	1506, 1940
AflIII	ACRYGT	1 cut(s)	667
AhlI	ACTAGT	1 cut(s)	347
AjnI	CCWGG	9 cut(s)	139, 148, 304, 1201, 1369, 1460, 1609, 2059, 2152
AjuI	GAANNNNNNNTTGG	4 cut(s)	22, 54, 1531, 1563
Alw21I	GWGCWC	4 cut(s)	9, 904, 1079, 1162
Alw26I	GTCTC	2 cut(s)	1105, 2185
Alw44I	GTGCAC	1 cut(s)	1075
AlwI	GGATC	7 cut(s)	188, 201, 603, 923, 1279, 1460, 1473
Aor13HI	TCCGGA	1 cut(s)	1933
Aor51HI	AGCGCT	1 cut(s)	911
AoxI	GGCC	6 cut(s)	144, 405, 586, 1231, 1282, 1896
ApaI	GGGCCC	1 cut(s)	148
ApaLI	GTGCAC	1 cut(s)	1075
ApeKI	GCWGC	5 cut(s)	1296, 1574, 1952, 2243, 2297
ApoI	RAATTY	2 cut(s)	518, 2371
AspLEI	GCGC	1 cut(s)	912
AspS9I	GGNCC	7 cut(s)	137, 144, 145, 405, 1044, 1122, 1745
AsuC2I	CCSGG	3 cut(s)	445, 732, 1709
AsuHPI	GGTGA	3 cut(s)	938, 1266, 1661
AsuII	TTCGAA	2 cut(s)	280, 1875
AvaII	GGWCC	4 cut(s)	137, 1044, 1122, 1745
BaeGI	GKGCMC	2 cut(s)	148, 1079
BalI	TGGCCA	2 cut(s)	1284, 1898
BamHI	GGATCC	2 cut(s)	193, 1465
BanII	GRGCYC	2 cut(s)	148, 1162
BbsI	GAAGAC	1 cut(s)	149
Bbv12I	GWGCWC	4 cut(s)	9, 904, 1079, 1162
BbvI	GCAGC	5 cut(s)	1283, 1586, 1939, 2255, 2309
BccI	CCATC	7 cut(s)	80, 640, 1113, 1556, 1609, 2156, 2276
BceAI	ACGGC	1 cut(s)	1218
BciT130I	CCWGG	9 cut(s)	141, 150, 306, 1203, 1371, 1462, 1611, 2061, 2154
BciVI	GTATCC	1 cut(s)	1193
BclI	TGATCA	1 cut(s)	619
BcnI	CCSGG	3 cut(s)	445, 732, 1709
BcoDI	GTCTC	2 cut(s)	1105, 2185
BcuI	ACTAGT	1 cut(s)	347
BfaI	CTAG	7 cut(s)	233, 348, 827, 1178, 1229, 1922, 2042
BfoI	RGCGCY	1 cut(s)	913
BfuI	GTATCC	1 cut(s)	1193
BglII	AGATCT	1 cut(s)	576
BisI	GCNGC	6 cut(s)	1297, 1575, 1761, 1953, 2244, 2298
BlsI	GCNGC	6 cut(s)	1298, 1576, 1762, 1954, 2245, 2299
BmcAI	AGTACT	1 cut(s)	656
Bme18I	GGWCC	4 cut(s)	137, 1044, 1122, 1745
BmgT120I	GGNCC	7 cut(s)	137, 144, 145, 405, 1044, 1122, 1745
BmrI	ACTGGG	2 cut(s)	1317, 1789
BmsI	GCATC	6 cut(s)	310, 712, 1606, 2008, 2086, 2374
BmuI	ACTGGG	2 cut(s)	1317, 1789
BoxI	GACNNNNGTC	1 cut(s)	1007
BpiI	GAAGAC	1 cut(s)	149
BplI	GAGNNNNNCTC	4 cut(s)	144, 176, 448, 480
BpmI	CTGGAG	3 cut(s)	171, 1224, 1593
Bpu14I	TTCGAA	2 cut(s)	280, 1875
BpuEI	CTTGAG	1 cut(s)	146
BpuMI	CCSGG	3 cut(s)	445, 732, 1709
BsaHI	GRCGYC	1 cut(s)	341
BsaI	GGTCTC	2 cut(s)	1105, 2185
BsaWI	WCCGGW	3 cut(s)	111, 128, 1933
BsaXI	ACNNNNNCTCC	2 cut(s)	1633, 1663
Bsc4I	CCNNNNNNNGG	2 cut(s)	1506, 1940
Bse1I	ACTGG	8 cut(s)	928, 1312, 1387, 1748, 1770, 1784, 1820, 1909
Bse3DI	GCAATG	2 cut(s)	173, 402
BseAI	TCCGGA	1 cut(s)	1933
BseBI	CCWGG	9 cut(s)	141, 150, 306, 1203, 1371, 1462, 1611, 2061, 2154
BseGI	GGATG	7 cut(s)	651, 1597, 1785, 1942, 2167, 2335, 2410
BseLI	CCNNNNNNNGG	2 cut(s)	1506, 1940
BseMI	GCAATG	2 cut(s)	173, 402
BseMII	CTCAG	3 cut(s)	504, 1188, 1673
BseNI	ACTGG	8 cut(s)	928, 1312, 1387, 1748, 1770, 1784, 1820, 1909
BseSI	GKGCMC	2 cut(s)	148, 1079
BseXI	GCAGC	5 cut(s)	1283, 1586, 1939, 2255, 2309
BsgI	GTGCAG	1 cut(s)	863
Bsh1236I	CGCG	1 cut(s)	528
Bsh1285I	CGRYCG	1 cut(s)	1917
BshFI	GGCC	6 cut(s)	146, 407, 588, 1233, 1284, 1898
BsiEI	CGRYCG	1 cut(s)	1917
BsiHKAI	GWGCWC	4 cut(s)	9, 904, 1079, 1162
BsiSI	CCGG	6 cut(s)	112, 129, 444, 732, 1708, 1934
BslFI	GGGAC	4 cut(s)	1015, 1057, 1522, 1796
BslI	CCNNNNNNNGG	2 cut(s)	1506, 1940
BsmAI	GTCTC	2 cut(s)	1105, 2185
BsmFI	GGGAC	4 cut(s)	1015, 1057, 1522, 1796
BsmI	GAATGC	1 cut(s)	1545
BsnI	GGCC	6 cut(s)	146, 407, 588, 1233, 1284, 1898
Bso31I	GGTCTC	2 cut(s)	1105, 2185
Bsp119I	TTCGAA	2 cut(s)	280, 1875
Bsp120I	GGGCCC	1 cut(s)	144
Bsp1286I	GDGCHC	5 cut(s)	9, 148, 904, 1079, 1162
Bsp13I	TCCGGA	1 cut(s)	1933
Bsp19I	CCATGG	1 cut(s)	1645
BspACI	CCGC	4 cut(s)	358, 839, 1142, 1760
BspANI	GGCC	6 cut(s)	146, 407, 588, 1233, 1284, 1898
BspCNI	CTCAG	3 cut(s)	505, 1189, 1674
BspEI	TCCGGA	1 cut(s)	1933
BspFNI	CGCG	1 cut(s)	528
BspPI	GGATC	7 cut(s)	188, 201, 603, 923, 1279, 1460, 1473
BspT104I	TTCGAA	2 cut(s)	280, 1875
BspTNI	GGTCTC	2 cut(s)	1105, 2185
BsrDI	GCAATG	2 cut(s)	173, 402
BsrI	ACTGG	8 cut(s)	928, 1312, 1387, 1748, 1770, 1784, 1820, 1909
BssNAI	GTATAC	1 cut(s)	603
BssNI	GRCGYC	1 cut(s)	341
BssT1I	CCWWGG	4 cut(s)	1645, 1893, 2184, 2356
Bst1107I	GTATAC	1 cut(s)	603
Bst2UI	CCWGG	9 cut(s)	141, 150, 306, 1203, 1371, 1462, 1611, 2061, 2154
Bst4CI	ACNGT	9 cut(s)	695, 1242, 1444, 1528, 1657, 1948, 2049, 2419, 2440
Bst6I	CTCTTC	3 cut(s)	982, 1347, 2309
BstACI	GRCGYC	1 cut(s)	341
BstAPI	GCANNNNNTGC	2 cut(s)	1718, 2018
BstBI	TTCGAA	2 cut(s)	280, 1875
BstC8I	GCNNGC	8 cut(s)	409, 586, 846, 1016, 1091, 1158, 1403, 2302
BstDEI	CTNAG	5 cut(s)	513, 1197, 1475, 1682, 1794
BstDSI	CCRYGG	2 cut(s)	1234, 1645
BstF5I	GGATG	7 cut(s)	651, 1597, 1785, 1942, 2167, 2335, 2410
BstFNI	CGCG	1 cut(s)	528
BstH2I	RGCGCY	1 cut(s)	913
BstHHI	GCGC	1 cut(s)	912
BstMAI	GTCTC	2 cut(s)	1105, 2185
BstMCI	CGRYCG	1 cut(s)	1917
BstMWI	GCNNNNNNNGC	5 cut(s)	845, 1574, 1718, 1961, 2018
BstNI	CCWGG	9 cut(s)	141, 150, 306, 1203, 1371, 1462, 1611, 2061, 2154
BstNSI	RCATGY	1 cut(s)	1093
BstPAI	GACNNNNGTC	1 cut(s)	1007
BstSLI	GKGCMC	2 cut(s)	148, 1079
BstUI	CGCG	1 cut(s)	528
BstV1I	GCAGC	5 cut(s)	1283, 1586, 1939, 2255, 2309
BstV2I	GAAGAC	1 cut(s)	149
BstX2I	RGATCY	3 cut(s)	193, 576, 1465
BstXI	CCANNNNNNTGG	1 cut(s)	1638
BstYI	RGATCY	3 cut(s)	193, 576, 1465
BstZ17I	GTATAC	1 cut(s)	603
BsuI	GTATCC	1 cut(s)	1193
BsuRI	GGCC	6 cut(s)	146, 407, 588, 1233, 1284, 1898
BtgI	CCRYGG	2 cut(s)	1234, 1645
BtsCI	GGATG	7 cut(s)	651, 1597, 1785, 1942, 2167, 2335, 2410
BtsI	GCAGTG	1 cut(s)	493
BtsIMutI	CAGTG	5 cut(s)	108, 493, 921, 1777, 1944
Cac8I	GCNNGC	8 cut(s)	409, 586, 846, 1016, 1091, 1158, 1403, 2302
CfoI	GCGC	1 cut(s)	912
Cfr13I	GGNCC	7 cut(s)	137, 144, 145, 405, 1044, 1122, 1745
CseI	GACGC	1 cut(s)	330
Csp6I	GTAC	6 cut(s)	109, 238, 655, 691, 1305, 1430
CviAII	CATG	8 cut(s)	179, 209, 739, 1090, 1646, 1961, 2038, 2401
CviQI	GTAC	6 cut(s)	109, 238, 655, 691, 1305, 1430
DdeI	CTNAG	5 cut(s)	513, 1197, 1475, 1682, 1794
DraI	TTTAAA	1 cut(s)	1905
DraIII	CACNNNGTG	1 cut(s)	32
EaeI	YGGCCR	2 cut(s)	1282, 1896
Eam1104I	CTCTTC	3 cut(s)	982, 1347, 2309
EarI	CTCTTC	3 cut(s)	982, 1347, 2309
Ecl136II	GAGCTC	1 cut(s)	1160
Eco130I	CCWWGG	4 cut(s)	1645, 1893, 2184, 2356
Eco147I	AGGCCT	1 cut(s)	588
Eco24I	GRGCYC	2 cut(s)	148, 1162
Eco31I	GGTCTC	2 cut(s)	1105, 2185
Eco32I	GATATC	2 cut(s)	494, 684
Eco47I	GGWCC	4 cut(s)	137, 1044, 1122, 1745
Eco47III	AGCGCT	1 cut(s)	911
Eco53kI	GAGCTC	1 cut(s)	1160
Eco57I	CTGAAG	3 cut(s)	375, 1115, 1707
EcoICRI	GAGCTC	1 cut(s)	1160
EcoO109I	RGGNCCY	3 cut(s)	144, 145, 1044
EcoRII	CCWGG	9 cut(s)	139, 148, 304, 1201, 1369, 1460, 1609, 2059, 2152
EcoRV	GATATC	2 cut(s)	494, 684
EcoT14I	CCWWGG	4 cut(s)	1645, 1893, 2184, 2356
EcoT38I	GRGCYC	2 cut(s)	148, 1162
ErhI	CCWWGG	4 cut(s)	1645, 1893, 2184, 2356
FaeI	CATG	8 cut(s)	182, 212, 742, 1093, 1649, 1964, 2041, 2404
FalI	AAGNNNNNCTT	6 cut(s)	1416, 1448, 1722, 1754, 2195, 2227
FaqI	GGGAC	4 cut(s)	1015, 1057, 1522, 1796
FatI	CATG	8 cut(s)	178, 208, 738, 1089, 1645, 1960, 2037, 2400
FauI	CCCGC	1 cut(s)	351
FauNDI	CATATG	1 cut(s)	316
FbaI	TGATCA	1 cut(s)	619
FblI	GTMKAC	1 cut(s)	602
Fnu4HI	GCNGC	6 cut(s)	1297, 1575, 1761, 1953, 2244, 2298
FokI	GGATG	7 cut(s)	658, 1584, 1772, 1949, 2174, 2322, 2417
FriOI	GRGCYC	2 cut(s)	148, 1162
Fsp4HI	GCNGC	6 cut(s)	1297, 1575, 1761, 1953, 2244, 2298
FspBI	CTAG	7 cut(s)	233, 348, 827, 1178, 1229, 1922, 2042
GlaI	GCGC	1 cut(s)	911
GluI	GCNGC	6 cut(s)	1297, 1575, 1761, 1953, 2244, 2298
GsuI	CTGGAG	3 cut(s)	171, 1224, 1593
HaeII	RGCGCY	1 cut(s)	913
HaeIII	GGCC	6 cut(s)	146, 407, 588, 1233, 1284, 1898
HapII	CCGG	6 cut(s)	112, 129, 444, 732, 1708, 1934
HgaI	GACGC	1 cut(s)	330
HhaI	GCGC	1 cut(s)	912
Hin1I	GRCGYC	1 cut(s)	341
Hin1II	CATG	8 cut(s)	182, 212, 742, 1093, 1649, 1964, 2041, 2404
Hin6I	GCGC	1 cut(s)	910
HinP1I	GCGC	1 cut(s)	910
HincII	GTYRAC	4 cut(s)	345, 973, 1488, 1800
HindII	GTYRAC	4 cut(s)	345, 973, 1488, 1800
HindIII	AAGCTT	3 cut(s)	624, 1399, 2395
HpaI	GTTAAC	2 cut(s)	973, 1488
HpaII	CCGG	6 cut(s)	112, 129, 444, 732, 1708, 1934
HphI	GGTGA	3 cut(s)	938, 1266, 1661
Hpy166II	GTNNAC	9 cut(s)	345, 353, 603, 973, 1077, 1238, 1488, 1531, 1800
Hpy188III	TCNNGA	3 cut(s)	233, 1664, 1934
Hpy8I	GTNNAC	9 cut(s)	345, 353, 603, 973, 1077, 1238, 1488, 1531, 1800
HpyAV	CCTTC	6 cut(s)	208, 469, 1090, 1352, 1498, 2059
HpyCH4III	ACNGT	9 cut(s)	695, 1242, 1444, 1528, 1657, 1948, 2049, 2419, 2440
HpyCH4IV	ACGT	2 cut(s)	667, 1484
HpyF10VI	GCNNNNNNNGC	5 cut(s)	845, 1574, 1718, 1961, 2018
HpyF3I	CTNAG	5 cut(s)	513, 1197, 1475, 1682, 1794
HpySE526I	ACGT	2 cut(s)	667, 1484
Hsp92I	GRCGYC	1 cut(s)	341
Hsp92II	CATG	8 cut(s)	182, 212, 742, 1093, 1649, 1964, 2041, 2404
HspAI	GCGC	1 cut(s)	910
Kpn2I	TCCGGA	1 cut(s)	1933
Ksp22I	TGATCA	1 cut(s)	619
KspAI	GTTAAC	2 cut(s)	973, 1488
LmnI	GCTCC	2 cut(s)	1612, 1641
Lsp1109I	GCAGC	5 cut(s)	1283, 1586, 1939, 2255, 2309
LweI	GCATC	6 cut(s)	310, 712, 1606, 2008, 2086, 2374
MaeI	CTAG	7 cut(s)	233, 348, 827, 1178, 1229, 1922, 2042
MaeII	ACGT	2 cut(s)	667, 1484
MaeIII	GTNAC	7 cut(s)	714, 919, 1448, 1649, 1657, 1775, 2198
MfeI	CAATTG	1 cut(s)	888
MflI	RGATCY	3 cut(s)	193, 576, 1465
MhlI	GDGCHC	5 cut(s)	9, 148, 904, 1079, 1162
MlsI	TGGCCA	2 cut(s)	1284, 1898
MluNI	TGGCCA	2 cut(s)	1284, 1898
MlyI	GAGTC	3 cut(s)	466, 1968, 2198
MmeI	TCCRAC	6 cut(s)	171, 221, 231, 533, 573, 2211
Mox20I	TGGCCA	2 cut(s)	1284, 1898
MroI	TCCGGA	1 cut(s)	1933
MscI	TGGCCA	2 cut(s)	1284, 1898
MseI	TTAA	7 cut(s)	972, 1434, 1487, 1698, 1904, 2238, 2443
MslI	CAYNNNNRTG	2 cut(s)	987, 1770
Msp20I	TGGCCA	2 cut(s)	1284, 1898
MspA1I	CMGCKG	1 cut(s)	290
MspI	CCGG	6 cut(s)	112, 129, 444, 732, 1708, 1934
MunI	CAATTG	1 cut(s)	888
Mva1269I	GAATGC	1 cut(s)	1545
MvaI	CCWGG	9 cut(s)	141, 150, 306, 1203, 1371, 1462, 1611, 2061, 2154
MvnI	CGCG	1 cut(s)	528
MwoI	GCNNNNNNNGC	5 cut(s)	845, 1574, 1718, 1961, 2018
NciI	CCSGG	3 cut(s)	445, 732, 1709
NcoI	CCATGG	1 cut(s)	1645
NdeI	CATATG	1 cut(s)	316
NlaIII	CATG	8 cut(s)	182, 212, 742, 1093, 1649, 1964, 2041, 2404
NmuCI	GTSAC	4 cut(s)	714, 919, 1649, 2198
NspI	RCATGY	1 cut(s)	1093
NspV	TTCGAA	2 cut(s)	280, 1875
PaeI	GCATGC	1 cut(s)	1093
PasI	CCCWGGG	1 cut(s)	305
PceI	AGGCCT	1 cut(s)	588
PcsI	WCGNNNNNNNCGW	1 cut(s)	338
PctI	GAATGC	1 cut(s)	1545
PfeI	GAWTC	9 cut(s)	38, 254, 1070, 1316, 1337, 1479, 1556, 2111, 2286
PfoI	TCCNGGA	1 cut(s)	443
PkrI	GCNGC	6 cut(s)	1298, 1576, 1762, 1954, 2245, 2299
Ple19I	CGATCG	1 cut(s)	1917
PleI	GAGTC	3 cut(s)	465, 1968, 2197
PpsI	GAGTC	3 cut(s)	465, 1968, 2197
PpuMI	RGGWCCY	1 cut(s)	1044
PshAI	GACNNNNGTC	1 cut(s)	1007
PsiI	TTATAA	2 cut(s)	756, 939
Psp124BI	GAGCTC	1 cut(s)	1162
Psp5II	RGGWCCY	1 cut(s)	1044
Psp6I	CCWGG	9 cut(s)	139, 148, 304, 1201, 1369, 1460, 1609, 2059, 2152
PspGI	CCWGG	9 cut(s)	139, 148, 304, 1201, 1369, 1460, 1609, 2059, 2152
PspOMI	GGGCCC	1 cut(s)	144
PspPI	GGNCC	7 cut(s)	137, 144, 145, 405, 1044, 1122, 1745
PspPPI	RGGWCCY	1 cut(s)	1044
PsuI	RGATCY	3 cut(s)	193, 576, 1465
PvuI	CGATCG	1 cut(s)	1917
PvuII	CAGCTG	1 cut(s)	290
RsaI	GTAC	6 cut(s)	110, 239, 656, 692, 1306, 1431
RsaNI	GTAC	6 cut(s)	109, 238, 655, 691, 1305, 1430
RseI	CAYNNNNRTG	2 cut(s)	987, 1770
SacI	GAGCTC	1 cut(s)	1162
SaqAI	TTAA	7 cut(s)	972, 1434, 1487, 1698, 1904, 2238, 2443
SatI	GCNGC	6 cut(s)	1297, 1575, 1761, 1953, 2244, 2298
Sau96I	GGNCC	7 cut(s)	137, 144, 145, 405, 1044, 1122, 1745
ScaI	AGTACT	1 cut(s)	656
SchI	GAGTC	3 cut(s)	466, 1968, 2198
SduI	GDGCHC	5 cut(s)	9, 148, 904, 1079, 1162
SfaNI	GCATC	6 cut(s)	310, 712, 1606, 2008, 2086, 2374
SfuI	TTCGAA	2 cut(s)	280, 1875
SinI	GGWCC	4 cut(s)	137, 1044, 1122, 1745
SmiMI	CAYNNNNRTG	2 cut(s)	987, 1770
SmlI	CTYRAG	1 cut(s)	161
SmoI	CTYRAG	1 cut(s)	161
SpeI	ACTAGT	1 cut(s)	347
SphI	GCATGC	1 cut(s)	1093
SseBI	AGGCCT	1 cut(s)	588
SsiI	CCGC	4 cut(s)	358, 839, 1142, 1760
SspMI	CTAG	7 cut(s)	233, 348, 827, 1178, 1229, 1922, 2042
SstI	GAGCTC	1 cut(s)	1162
StuI	AGGCCT	1 cut(s)	588
StyI	CCWWGG	4 cut(s)	1645, 1893, 2184, 2356
TaaI	ACNGT	9 cut(s)	695, 1242, 1444, 1528, 1657, 1948, 2049, 2419, 2440
TaiI	ACGT	2 cut(s)	670, 1487
TaqI	TCGA	8 cut(s)	280, 455, 686, 720, 1588, 1875, 1913, 1917
TaqII	GACCGA	1 cut(s)	2167
TatI	WGTACW	2 cut(s)	654, 690
TauI	GCSGC	1 cut(s)	1763
TfiI	GAWTC	9 cut(s)	38, 254, 1070, 1316, 1337, 1479, 1556, 2111, 2286
Tru1I	TTAA	7 cut(s)	972, 1434, 1487, 1698, 1904, 2238, 2443
Tru9I	TTAA	7 cut(s)	972, 1434, 1487, 1698, 1904, 2238, 2443
TscAI	CASTG	5 cut(s)	108, 493, 928, 1777, 1951
TseFI	GTSAC	4 cut(s)	714, 919, 1649, 2198
TseI	GCWGC	5 cut(s)	1296, 1574, 1952, 2243, 2297
Tsp45I	GTSAC	4 cut(s)	714, 919, 1649, 2198
TspDTI	ATGAA	8 cut(s)	134, 653, 755, 930, 1949, 2327, 2389, 2421
TspGWI	ACGGA	1 cut(s)	1240
TspRI	CASTG	5 cut(s)	108, 493, 928, 1777, 1951
VneI	GTGCAC	1 cut(s)	1075
VpaK11BI	GGWCC	4 cut(s)	137, 1044, 1122, 1745
XapI	RAATTY	2 cut(s)	518, 2371
XbaI	TCTAGA	1 cut(s)	232
XceI	RCATGY	1 cut(s)	1093
XmiI	GTMKAC	1 cut(s)	602
XspI	CTAG	7 cut(s)	233, 348, 827, 1178, 1229, 1922, 2042
ZrmI	AGTACT	1 cut(s)	656

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.