MD03G1028500.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012309
Physical Location & Seq
Forward (+)
2334388 .. 2339320
4933 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1028500.v1.1.491

Sequence Viewer

Length: 3432 bp
ATGTTGTCCTCAACGGCTAGGCCGAAGCAATTCGCTCTAGGTTCCCACGCAGTTTCAAAGCTCAGAGCTTTCCATGCTTTCTCCCAGAAAAATCCCAATCCGAATCTCGGAGCCGACGGCGCCAAAAGCATCGCTCGTGAAAATCCACAAGCCTCCGGTTCAGAAATGGAGAAGAGCATATATGCAATTCTGACCATTGATCGTTGGGTGTCACTGAACCACATGGAGTATAGGATGGCTTCTCTCAGACCAGTTCATGGAAAGCTGGCTTTGAAGTTTCTAAATTGGGTCATCGAACAATCTGGTTTGGAACTCAACCATTTGACTCATATACTTTCTGTCACAACCCATATACTTGTTCGAGCTAGAATGTATGATTCTGCCAAATCAGTTTTGGGGCATTTGTTGCAAATGGATTTTGGCCCGAAATCTGTTTTCAGTGCTCTAATGGACACCTACCCGCTTTGCAATTCAAACCCTTCGGTTTTTGACCTTCTAATTAGAGTATATTTGAGGGAAGGAATGGCTGGTTGTGCTGTAGAGGCTTCCTATTTGATGGGTTTGAGGGGATTTAGACCGTCTACTTGTACTTGTAATATGATCCTAGCTTTGTTGGCAAAGGACCAGAAAGCAGCATCAGTATGGTCTTTTTTCAAAGAAATGCTTGCTCAGAAGGTATGTCCTGATGTTGCTACTTTTAATATATTGATTGGTGTTTTATGTGCGGAAGGGAAGCTTAAGAGAGCTAGTTATCTTTTGAGAAAGATGGAAAAGAGTGGCTATGCTCCGAACACAGTTACTTATAACACTTTGCTCAATTGGTACTGCAAAAAGGGGAGGTATAAAGCAGCCTTTGAGCTTATTGATCATATGGGTTCAAAGGGTATAGAAGCAGATGTGTGTACATATAACATGCTTATTGGCGATTTATGTAGAAATAATAGAAGTGCAAAAAGTTATCTGCTGTTGAAGAAAATGAGAAGGAAGAAGTTATCTCCAAATGAGGTTACTTACAATACTCTTATTAACGGGTTTGTTAAGGAGGGGAAAATTGATGTTGCTACCAGGGTTTTTGACGAGATGTCTAGGTTTAATCTATCACCAAATTCTGTTACTTTCAATGCTTTGATTAATGGCCTCTCTCAAAATGGTAAGCTTGACGAAGCTTTCAGACTTTTCGACATGATGGAAGCAACGGGAATAAGACCTACTGAAGTCAGCTATGGGGCTCTTTTGAATGGGCTGTGTAAGCATGCCAAGTTTGATTTAGCAAGAAGTGTTATTGAGAAATTGCGGATGGATGGGATTGCTGTTAGTTGTATTATGTATACAGAGATCATGGATGGGTTATGCAAAAATGGGTTGCTTGATGAAGCTATGAAATTCTTTAATATGATGGTCCAGGATGGCGTGAATCCCGATATTGTAGCATTTTCAGTGCTCATAAATGGACTTTGCAGAGCTGGGAAGATGAAACATGCGAAGGAGATGGTATGTAAAATCTATAAAGCTGGCCTTTCACCGAATAGAATAATATATTCTACTTTAATCTATAACTCTTGCAAGATGGGTAACATAGCGGAAGCATGTAACATTTATGCAGCTATGAACTGCACCGGTCATGGTGCTGACCATTTCACATGTAATCTATTGGTTGGTTCCCTTTGTGAAGCTGGAAAGCTGGGAGTGGCAGAAGATTTCATGTGTCATATGAGCAGTATCGGTCTTGATCCCAATTCTGTTACTTTCGATTGTCTAATAAATGGCTACGGAAGCATGGGAAATGGATTGAAAGCACTCTCTATATTTGATGAAATGATTAAATCAGGGCATCATCCAAGCCCATTCACATATGGAAGTATACTGAAAGGGCTATGCAAGGGTGGAAATTTGGGGGAGGCAAAGAAATTTTTGAAGAAACTCCACGGCATTCCATATGCTGTTGATACTGTTGTGTACAATACAATACTGCATGAGACATGTAAATCTGGAAATCTGCATGAGGCTGTTTCCCTTCTTGATGAGATAGTTGAGAATAACGTTCTGCCTGATAATTATACGTATGGTAGCCTTCTTGCTGGGTTATGTTGGAAGGGAAAAATTGTTGCTGCCATCCTGTTGTTCGGGAAAGTGATGGAAAAGGTGACTCTCTCACAAAGTGCAACGATGTATACCTGTCTAGTTGATGGCCTTTTCAAGACCGGCCAGTCAAAGGCTGCTTCATATCTCTTTGAAGAGATGGAAAACAAAGGTTTATACTCAGATACTATTGCTTGCAATGTAATGATAGATGGATACTCAAGGATGGGAAAAATGACGAAGGCAAAAGAACTCTTTTCAACTATGGGGAGCCAAAGATTGCCCCCTAATTTGGATACATATAATATTCTCTTGCATGGATACTCGAAAAACCGGGACCTCTTGACATGCTCTGGTTTATACAAGAAAATGATCCGGGCTGGATTATATCCTGATAAATTGACATTCCACTCTCTTATTCAGGGATTATGTGAGTCTGGTATGTTGGATATTGGCCGCAAAATGTTGAATAAGATGATAATGGAAGGTGCTGCAGCTGATCAGTTAACATTTAACATGCTTATTAGAAAGTTTTCTGAGACGGGTAAGGTGGGGATGGCCTGTGATCTTGTTGGGGTCATGGATATGTTAGGAGTTTCTGCTAATATAGAAACCCATGGCGCAATCTTGAATGGACTATTTAGATGCTCGAATTTCCAAGCATCCTGTGTTCTTCTTTATGAAATGTTGGCCAAAGGTTTCGCTCCTGAGCATACACATTATATCACCCTAATTAATGGTATGTGTAGAGTGGGAGATATACAAGGAGCATTCGAGCTAAAAGATCAGATGGGAGCTCTTGGTGTCACTTCACGTGACATTGCTGAGAGTGCTATGGTTAGAGGGCTTGCAAAATGTGGGAAGATTGAAGACGCCATGCTTGTTTTTGATCACATGCTTCGGATGAAACTCATTCCAACGACTGCTACTTTTACTACTTTGATGCACATGTTCTGTAAAGAAGCCAACCTTGAAGAGGCTCTAAAACTGAGGGGTGTAATGGAACGTTGTGGTCTGAAGCTTGATGTTCCAGTGTTTAATGTTCTGATTTCAGGCCTTTGTGCTAATGGCGATGTTGTGGCTGCATTTGCGCTTTATAAGGAGATGAAACAAAGAGGGCTGCTGCCCAACGTGACAACATATACTCTGCTCATTGGTGCTGTTTCTGCCGAAAATAATCTTATCGAAGGTGAACAACTTTTGGAAGATTTATGTGAAAGGGGATTGATATCTGGAAATTGGGATGGAAATGCCCTAACGCTACATGAGGTTTTGATGATTTCAATGCAAAAACTCAATTCTTTGAGGCGCATAAAAGGAACTAATACTAAGACGTGTAGTGAATCAACATAA

Protein Analysis

1144

Amino Acids

126.0

Weight (kDa)

8.69

Isoelectric Point (pI)

30.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 193 - 241 6.1e-10 PPR repeat family
PPR_long PF17177 208 - 345 1.2e-12 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 218 - 276 1e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 226 - 257 4.8e-09 PPR repeat
PPR_2 PF13041 228 - 273 4.4e-13 PPR repeat family
PPR_3 PF13812 252 - 308 5.9e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 260 - 291 1.6e-10 PPR repeat
PPR_2 PF13041 263 - 312 1.8e-18 PPR repeat family
PPR PF01535 266 - 296 1.9e-07 PPR repeat
PPR_3 PF13812 287 - 347 1.3e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 294 - 327 1.9e-06 PPR repeat
PPR_2 PF13041 299 - 347 1.7e-13 PPR repeat family
PPR_long PF17177 317 - 432 5.9e-11 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 322 - 378 1.6e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 330 - 362 4.5e-12 PPR repeat
PPR_2 PF13041 333 - 380 2.3e-17 PPR repeat family
PPR PF01535 336 - 365 1.6e-07 PPR repeat
TPR_24 PF23276 348 - 414 7.6e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 356 - 413 9.3e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 365 - 397 2.3e-12 PPR repeat
PPR_2 PF13041 368 - 417 5.2e-17 PPR repeat family
PPR PF01535 371 - 400 2.5e-08 PPR repeat
TPR_24 PF23276 412 - 535 4.4e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 427 - 483 5e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 435 - 467 3.2e-07 PPR repeat
PPR_2 PF13041 441 - 487 2.5e-14 PPR repeat family
PPR PF01535 442 - 471 8.5e-07 PPR repeat
PPR_3 PF13812 466 - 517 1.4e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 469 - 499 2e-08 PPR repeat
PPR_2 PF13041 473 - 522 1e-08 PPR repeat family
PPR_2 PF13041 544 - 590 5.7e-08 PPR repeat family
TPR_24 PF23276 559 - 673 3e-07 Fungal tetratrico peptide repeats
PPR_1 PF12854 575 - 606 7.7e-06 PPR repeat
PPR_2 PF13041 578 - 627 2e-13 PPR repeat family
PPR PF01535 581 - 610 1.8e-06 PPR repeat
PPR_1 PF12854 610 - 640 2.1e-06 PPR repeat
PPR_1 PF12854 649 - 676 3.5e-06 PPR repeat
PPR_2 PF13041 649 - 696 7.5e-14 PPR repeat family
PPR_3 PF13812 743 - 803 2.6e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 755 - 803 3.5e-13 PPR repeat family
PPR_2 PF13041 789 - 837 7.6e-11 PPR repeat family
PPR_1 PF12854 821 - 852 1e-05 PPR repeat
PPR_1 PF12854 925 - 957 3.8e-06 PPR repeat
TPR_24 PF23276 970 - 1062 4.3e-06 Fungal tetratrico peptide repeats
PPR_2 PF13041 971 - 1013 4.1e-08 PPR repeat family
PPR_3 PF13812 1027 - 1081 2e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 1031 - 1063 1.8e-07 PPR repeat
PPR_2 PF13041 1035 - 1080 1.3e-14 PPR repeat family
PPR PF01535 1038 - 1067 7.8e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012309)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55840 AT5G55840 AT5G55840
fragaria_vesca FvH4_3g42930
malus_domestica MD03G1028500.v1.1
prunus_persica Prupe.6G024500_v2.0.a1 Prupe.6G024500_v2.0.a1
pyrus_communis pycom03g02310
rosa_chinensis RchiOBHm_Chr5g0076971
rosa_laevigata RLG00000036637
rosa_multiflora Rmu_sc0004655.1_g000020
rosa_roxburghii Rroxscaffold_1G00005180
rosa_rugosa Rorug05G0449800 Rorug05G0449900
rosa_samantha Rh5AG504500 Rh5BG526200 Rh5CG550100 Rh5DG538400
rosa_wichuraiana Rw5G047180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 804, 3177
AasI GACNNNNNNGTC 1 cut(s) 2206
AccB1I GGYRCC 1 cut(s) 119
AccB7I CCANNNNNTGG 1 cut(s) 257
AccI GTMKAC 4 cut(s) 581, 1328, 1861, 2171
AciI CCGC 5 cut(s) 461, 725, 1294, 1580, 2536
AclI AACGTT 2 cut(s) 2040, 3085
AclWI GGATC 3 cut(s) 595, 1724, 2446
AcoI YGGCCR 3 cut(s) 2203, 2533, 2769
AcsI RAATTY 5 cut(s) 1105, 1382, 1888, 1907, 2731
AcuI CTGAAG 2 cut(s) 1233, 3116
AcvI CACGTG 1 cut(s) 2894
AcyI GRCGYC 2 cut(s) 120, 2952
AdeI CACNNNGTG 1 cut(s) 2159
AfaI GTAC 4 cut(s) 589, 824, 904, 1958
AfiI CCNNNNNNNGG 5 cut(s) 107, 257, 2212, 2371, 3055
AflII CTTAAG 1 cut(s) 737
AflIII ACRYGT 4 cut(s) 1640, 1979, 3027, 3413
AgeI ACCGGT 1 cut(s) 1616
AhdI GACNNNNNGTC 1 cut(s) 1081
AjiI CACGTC 1 cut(s) 3414
AjnI CCWGG 2 cut(s) 1064, 1401
Alw21I GWGCWC 3 cut(s) 445, 1443, 2878
Alw26I GTCTC 2 cut(s) 1970, 2612
AlwI GGATC 3 cut(s) 595, 1724, 2446
ApoI RAATTY 5 cut(s) 1105, 1382, 1888, 1907, 2731
AseI ATTAAT 2 cut(s) 1131, 2814
AsiGI ACCGGT 1 cut(s) 1616
Asp700I GAANNNNTTC 2 cut(s) 29, 2611
AspLEI GCGC 4 cut(s) 122, 2702, 3172, 3390
AspS9I GGNCC 4 cut(s) 422, 622, 1399, 2416
AsuC2I CCSGG 2 cut(s) 2414, 2456
AsuHPI GGTGA 5 cut(s) 1092, 1512, 2155, 2797, 3281
AvaII GGWCC 3 cut(s) 622, 1399, 2416
BaeI ACNNNNGTAYC 2 cut(s) 1938, 1971
BalI TGGCCA 1 cut(s) 2771
BanI GGYRCC 1 cut(s) 119
BanII GRGCYC 2 cut(s) 1231, 2878
BauI CACGAG 1 cut(s) 135
BbrPI CACGTG 1 cut(s) 2894
BbsI GAAGAC 1 cut(s) 2955
Bbv12I GWGCWC 3 cut(s) 445, 1443, 2878
BceAI ACGGC 3 cut(s) 30, 133, 1942
BciT130I CCWGG 2 cut(s) 1066, 1403
BciVI GTATCC 3 cut(s) 2288, 2368, 2393
BclI TGATCA 3 cut(s) 865, 2578, 2968
BcnI CCSGG 2 cut(s) 2414, 2456
BcoDI GTCTC 2 cut(s) 1970, 2612
BfaI CTAG 7 cut(s) 18, 38, 366, 605, 747, 1086, 2180
BfmI CTRYAG 2 cut(s) 537, 2571
BfoI RGCGCY 1 cut(s) 123
BfrI CTTAAG 1 cut(s) 737
BfuI GTATCC 3 cut(s) 2288, 2368, 2393
Bme1390I CCNGG 4 cut(s) 1066, 1403, 2414, 2456
Bme18I GGWCC 3 cut(s) 622, 1399, 2416
BmeRI GACNNNNNGTC 1 cut(s) 1081
BmgBI CACGTC 1 cut(s) 3414
BmgT120I GGNCC 4 cut(s) 422, 622, 1399, 2416
BmiI GGNNCC 6 cut(s) 43, 112, 121, 1660, 2351, 2417
BmrFI CCNGG 4 cut(s) 1066, 1403, 2414, 2456
BmsI GCATC 6 cut(s) 138, 644, 1840, 2714, 2750, 3012
BpiI GAAGAC 1 cut(s) 2955
Bpu10I CCTNAGC 1 cut(s) 2787
BpuEI CTTGAG 1 cut(s) 2284
BpuMI CCSGG 2 cut(s) 2414, 2456
BsaAI YACGTR 2 cut(s) 2061, 2894
BsaBI GATNNNNATC 1 cut(s) 3307
BsaHI GRCGYC 2 cut(s) 120, 2952
BsaJI CCNNGG 3 cut(s) 1065, 1924, 2695
BsaWI WCCGGW 2 cut(s) 155, 1616
Bsc4I CCNNNNNNNGG 5 cut(s) 107, 257, 2212, 2371, 3055
Bse118I RCCGGY 2 cut(s) 1616, 2201
Bse1I ACTGG 3 cut(s) 251, 2206, 3110
Bse3DI GCAATG 2 cut(s) 2284, 2898
Bse8I GATNNNNATC 1 cut(s) 3307
BseBI CCWGG 2 cut(s) 1066, 1403
BseDI CCNNGG 3 cut(s) 1065, 1924, 2695
BseJI GATNNNNATC 1 cut(s) 3307
BseLI CCNNNNNNNGG 5 cut(s) 107, 257, 2212, 2371, 3055
BseMI GCAATG 2 cut(s) 2284, 2898
BseMII CTCAG 8 cut(s) 76, 259, 683, 2274, 2607, 2778, 2895, 3059
BseNI ACTGG 3 cut(s) 251, 2206, 3110
BseYI CCCAGC 3 cut(s) 1463, 1681, 2078
BsgI GTGCAG 1 cut(s) 1597
BshNI GGYRCC 1 cut(s) 119
BshTI ACCGGT 1 cut(s) 1616
BsiHKAI GWGCWC 3 cut(s) 445, 1443, 2878
BsiSI CCGG 5 cut(s) 156, 1617, 2202, 2413, 2455
BslFI GGGAC 1 cut(s) 2429
BslI CCNNNNNNNGG 5 cut(s) 107, 257, 2212, 2371, 3055
BsmAI GTCTC 2 cut(s) 1970, 2612
BsmBI CGTCTC 1 cut(s) 2612
BsmFI GGGAC 1 cut(s) 2429
BsmI GAATGC 2 cut(s) 1929, 2849
Bsp1286I GDGCHC 4 cut(s) 445, 1231, 1443, 2878
Bsp1407I TGTACA 2 cut(s) 902, 1956
Bsp19I CCATGG 1 cut(s) 2695
BspACI CCGC 5 cut(s) 461, 725, 1294, 1580, 2536
BspCNI CTCAG 8 cut(s) 75, 258, 682, 2273, 2608, 2779, 2896, 3060
BspLI GGNNCC 6 cut(s) 43, 112, 121, 1660, 2351, 2417
BspMAI CTGCAG 1 cut(s) 2575
BspPI GGATC 3 cut(s) 595, 1724, 2446
BspQI GCTCTTC 1 cut(s) 167
BspT107I GGYRCC 1 cut(s) 119
BspTI CTTAAG 1 cut(s) 737
BsrDI GCAATG 2 cut(s) 2284, 2898
BsrFI RCCGGY 2 cut(s) 1616, 2201
BsrGI TGTACA 2 cut(s) 902, 1956
BsrI ACTGG 3 cut(s) 251, 2206, 3110
BssAI RCCGGY 2 cut(s) 1616, 2201
BssECI CCNNGG 3 cut(s) 1065, 1924, 2695
BssNAI GTATAC 3 cut(s) 1329, 1862, 2172
BssNI GRCGYC 2 cut(s) 120, 2952
BssSI CACGAG 1 cut(s) 135
BssT1I CCWWGG 1 cut(s) 2695
Bst1107I GTATAC 3 cut(s) 1329, 1862, 2172
Bst2BI CACGAG 1 cut(s) 135
Bst2UI CCWGG 2 cut(s) 1066, 1403
Bst4CI ACNGT 3 cut(s) 579, 796, 1951
Bst6I CTCTTC 3 cut(s) 167, 2229, 3048
BstACI GRCGYC 2 cut(s) 120, 2952
BstAFI CTTAAG 1 cut(s) 737
BstAPI GCANNNNNTGC 1 cut(s) 406
BstAUI TGTACA 2 cut(s) 902, 1956
BstBAI YACGTR 2 cut(s) 2061, 2894
BstC8I GCNNGC 6 cut(s) 267, 666, 1254, 1513, 2275, 2928
BstDEI CTNAG 9 cut(s) 62, 245, 669, 2260, 2616, 2787, 2904, 3068, 3408
BstDSI CCRYGG 2 cut(s) 1924, 2695
BstENI CCTNNNNNAGG 1 cut(s) 3053
BstH2I RGCGCY 1 cut(s) 123
BstHHI GCGC 4 cut(s) 122, 2702, 3172, 3390
BstMAI GTCTC 2 cut(s) 1970, 2612
BstNI CCWGG 2 cut(s) 1066, 1403
BstSCI CCNGG 4 cut(s) 1064, 1401, 2412, 2454
BstSFI CTRYAG 2 cut(s) 537, 2571
BstSNI TACGTA 1 cut(s) 2061
BstV2I GAAGAC 1 cut(s) 2955
BstZ17I GTATAC 3 cut(s) 1329, 1862, 2172
BsuI GTATCC 3 cut(s) 2288, 2368, 2393
BtgI CCRYGG 2 cut(s) 1924, 2695
BtgZI GCGATG 2 cut(s) 115, 3165
BtrI CACGTC 1 cut(s) 3414
BtsIMutI CAGTG 4 cut(s) 212, 445, 1443, 3117
Cac8I GCNNGC 6 cut(s) 267, 666, 1254, 1513, 2275, 2928
CfoI GCGC 4 cut(s) 122, 2702, 3172, 3390
Cfr10I RCCGGY 2 cut(s) 1616, 2201
Cfr13I GGNCC 4 cut(s) 422, 622, 1399, 2416
CseI GACGC 1 cut(s) 2960
Csp6I GTAC 4 cut(s) 588, 823, 903, 1957
CspAI ACCGGT 1 cut(s) 1616
CviQI GTAC 4 cut(s) 588, 823, 903, 1957
DdeI CTNAG 9 cut(s) 62, 245, 669, 2260, 2616, 2787, 2904, 3068, 3408
DinI GGCGCC 1 cut(s) 121
DraIII CACNNNGTG 1 cut(s) 2159
DrdI GACNNNNNNGTC 1 cut(s) 2206
DriI GACNNNNNGTC 1 cut(s) 1081
DseDI GACNNNNNNGTC 1 cut(s) 2206
EaeI YGGCCR 3 cut(s) 2203, 2533, 2769
Eam1104I CTCTTC 3 cut(s) 167, 2229, 3048
Eam1105I GACNNNNNGTC 1 cut(s) 1081
EarI CTCTTC 3 cut(s) 167, 2229, 3048
Ecl136II GAGCTC 1 cut(s) 2876
Eco105I TACGTA 1 cut(s) 2061
Eco130I CCWWGG 1 cut(s) 2695
Eco147I AGGCCT 1 cut(s) 3135
Eco24I GRGCYC 2 cut(s) 1231, 2878
Eco32I GATATC 1 cut(s) 3309
Eco47I GGWCC 3 cut(s) 622, 1399, 2416
Eco53kI GAGCTC 1 cut(s) 2876
Eco57I CTGAAG 2 cut(s) 1233, 3116
Eco72I CACGTG 1 cut(s) 2894
EcoICRI GAGCTC 1 cut(s) 2876
EcoNI CCTNNNNNAGG 1 cut(s) 3053
EcoO109I RGGNCCY 1 cut(s) 2416
EcoRII CCWGG 2 cut(s) 1064, 1401
EcoRV GATATC 1 cut(s) 3309
EcoT14I CCWWGG 1 cut(s) 2695
EcoT38I GRGCYC 2 cut(s) 1231, 2878
EgeI GGCGCC 1 cut(s) 121
EheI GGCGCC 1 cut(s) 121
ErhI CCWWGG 1 cut(s) 2695
Esp3I CGTCTC 1 cut(s) 2612
FalI AAGNNNNNCTT 8 cut(s) 648, 680, 720, 752, 1500, 1532, 3033, 3065
FaqI GGGAC 1 cut(s) 2429
FauI CCCGC 1 cut(s) 468
FauNDI CATATG 4 cut(s) 870, 1710, 1852, 1936
FbaI TGATCA 3 cut(s) 865, 2578, 2968
FblI GTMKAC 4 cut(s) 581, 1328, 1861, 2171
FriOI GRGCYC 2 cut(s) 1231, 2878
FspBI CTAG 7 cut(s) 18, 38, 366, 605, 747, 1086, 2180
GlaI GCGC 4 cut(s) 121, 2701, 3171, 3389
GsaI CCCAGC 3 cut(s) 1467, 1685, 2082
HaeII RGCGCY 1 cut(s) 123
HapII CCGG 5 cut(s) 156, 1617, 2202, 2413, 2455
HgaI GACGC 1 cut(s) 2960
HhaI GCGC 4 cut(s) 122, 2702, 3172, 3390
Hin1I GRCGYC 2 cut(s) 120, 2952
Hin6I GCGC 4 cut(s) 120, 2700, 3170, 3388
HinP1I GCGC 4 cut(s) 120, 2700, 3170, 3388
HincII GTYRAC 1 cut(s) 2586
HindII GTYRAC 1 cut(s) 2586
HindIII AAGCTT 4 cut(s) 734, 1154, 1164, 3098
HinfI GANTC 7 cut(s) 103, 325, 377, 1414, 2146, 2513, 3422
HpaI GTTAAC 1 cut(s) 2586
HpaII CCGG 5 cut(s) 156, 1617, 2202, 2413, 2455
HphI GGTGA 5 cut(s) 1092, 1512, 2155, 2797, 3281
Hpy166II GTNNAC 8 cut(s) 582, 903, 1329, 1862, 1957, 2172, 2586, 3272
Hpy8I GTNNAC 8 cut(s) 582, 903, 1329, 1862, 1957, 2172, 2586, 3272
Hpy99I CGWCG 1 cut(s) 119
HpyCH4III ACNGT 3 cut(s) 579, 796, 1951
HpyCH4IV ACGT 6 cut(s) 2040, 2060, 2893, 3085, 3210, 3413
HpyF3I CTNAG 9 cut(s) 62, 245, 669, 2260, 2616, 2787, 2904, 3068, 3408
HpySE526I ACGT 6 cut(s) 2040, 2060, 2893, 3085, 3210, 3413
Hsp92I GRCGYC 2 cut(s) 120, 2952
HspAI GCGC 4 cut(s) 120, 2700, 3170, 3388
KasI GGCGCC 1 cut(s) 119
Ksp22I TGATCA 3 cut(s) 865, 2578, 2968
KspAI GTTAAC 1 cut(s) 2586
LguI GCTCTTC 1 cut(s) 167
LmnI GCTCC 6 cut(s) 110, 790, 2349, 2788, 2846, 2873
LweI GCATC 6 cut(s) 138, 644, 1840, 2714, 2750, 3012
MaeI CTAG 7 cut(s) 18, 38, 366, 605, 747, 1086, 2180
MaeII ACGT 6 cut(s) 2040, 2060, 2893, 3085, 3210, 3413
MfeI CAATTG 1 cut(s) 817
MhlI GDGCHC 4 cut(s) 445, 1231, 1443, 2878
MlsI TGGCCA 1 cut(s) 2771
MluNI TGGCCA 1 cut(s) 2771
Mly113I GGCGCC 1 cut(s) 120
MlyI GAGTC 3 cut(s) 319, 2140, 2522
MmeI TCCRAC 3 cut(s) 2069, 2505, 3020
Mox20I TGGCCA 1 cut(s) 2771
MroXI GAANNNNTTC 2 cut(s) 29, 2611
MscI TGGCCA 1 cut(s) 2771
MslI CAYNNNNRTG 2 cut(s) 640, 2663
Msp20I TGGCCA 1 cut(s) 2771
MspA1I CMGCKG 1 cut(s) 2576
MspCI CTTAAG 1 cut(s) 737
MspI CCGG 5 cut(s) 156, 1617, 2202, 2413, 2455
MspR9I CCNGG 4 cut(s) 1066, 1403, 2414, 2456
MunI CAATTG 1 cut(s) 817
Mva1269I GAATGC 2 cut(s) 1929, 2849
MvaI CCWGG 2 cut(s) 1066, 1403
NarI GGCGCC 1 cut(s) 120
NciI CCSGG 2 cut(s) 2414, 2456
NcoI CCATGG 1 cut(s) 2695
NdeI CATATG 4 cut(s) 870, 1710, 1852, 1936
NlaIV GGNNCC 6 cut(s) 43, 112, 121, 1660, 2351, 2417
NmuCI GTSAC 6 cut(s) 210, 340, 2143, 2884, 2894, 3211
PaeI GCATGC 1 cut(s) 1256
PceI AGGCCT 1 cut(s) 3135
PciI ACATGT 3 cut(s) 1640, 1979, 3027
PciSI GCTCTTC 1 cut(s) 167
PcsI WCGNNNNNNNCGW 1 cut(s) 20
PctI GAATGC 2 cut(s) 1929, 2849
PdmI GAANNNNTTC 2 cut(s) 29, 2611
PfeI GAWTC 4 cut(s) 103, 377, 1414, 3422
PflMI CCANNNNNTGG 1 cut(s) 257
PfoI TCCNGGA 1 cut(s) 1401
PinAI ACCGGT 1 cut(s) 1616
PleI GAGTC 3 cut(s) 319, 2140, 2521
PluTI GGCGCC 1 cut(s) 123
PmaCI CACGTG 1 cut(s) 2894
PmlI CACGTG 1 cut(s) 2894
PpsI GAGTC 3 cut(s) 319, 2140, 2521
Ppu21I YACGTR 2 cut(s) 2061, 2894
PpuMI RGGWCCY 1 cut(s) 2416
PscI ACATGT 3 cut(s) 1640, 1979, 3027
PshBI ATTAAT 2 cut(s) 1131, 2814
PsiI TTATAA 2 cut(s) 804, 3177
Psp124BI GAGCTC 1 cut(s) 2878
Psp1406I AACGTT 2 cut(s) 2040, 3085
Psp5II RGGWCCY 1 cut(s) 2416
Psp6I CCWGG 2 cut(s) 1064, 1401
PspCI CACGTG 1 cut(s) 2894
PspFI CCCAGC 3 cut(s) 1463, 1681, 2078
PspGI CCWGG 2 cut(s) 1064, 1401
PspN4I GGNNCC 6 cut(s) 43, 112, 121, 1660, 2351, 2417
PspPI GGNCC 4 cut(s) 422, 622, 1399, 2416
PspPPI RGGWCCY 1 cut(s) 2416
PstI CTGCAG 1 cut(s) 2575
PvuII CAGCTG 1 cut(s) 2576
RsaI GTAC 4 cut(s) 589, 824, 904, 1958
RsaNI GTAC 4 cut(s) 588, 823, 903, 1957
RseI CAYNNNNRTG 2 cut(s) 640, 2663
SacI GAGCTC 1 cut(s) 2878
SapI GCTCTTC 1 cut(s) 167
Sau96I GGNCC 4 cut(s) 422, 622, 1399, 2416
SchI GAGTC 3 cut(s) 319, 2140, 2522
ScrFI CCNGG 4 cut(s) 1066, 1403, 2414, 2456
SduI GDGCHC 4 cut(s) 445, 1231, 1443, 2878
SfaNI GCATC 6 cut(s) 138, 644, 1840, 2714, 2750, 3012
SfcI CTRYAG 2 cut(s) 537, 2571
SfoI GGCGCC 1 cut(s) 121
SinI GGWCC 3 cut(s) 622, 1399, 2416
SmiMI CAYNNNNRTG 2 cut(s) 640, 2663
SmlI CTYRAG 2 cut(s) 737, 2299
SmoI CTYRAG 2 cut(s) 737, 2299
SnaBI TACGTA 1 cut(s) 2061
SphI GCATGC 1 cut(s) 1256
SseBI AGGCCT 1 cut(s) 3135
SsiI CCGC 5 cut(s) 461, 725, 1294, 1580, 2536
SspDI GGCGCC 1 cut(s) 119
SspI AATATT 1 cut(s) 2386
SspMI CTAG 7 cut(s) 18, 38, 366, 605, 747, 1086, 2180
SstI GAGCTC 1 cut(s) 2878
StuI AGGCCT 1 cut(s) 3135
StyD4I CCNGG 4 cut(s) 1064, 1401, 2412, 2454
StyI CCWWGG 1 cut(s) 2695
TaaI ACNGT 3 cut(s) 579, 796, 1951
TaiI ACGT 6 cut(s) 2043, 2063, 2896, 3088, 3213, 3416
TaqI TCGA 8 cut(s) 294, 361, 1179, 1749, 2405, 2729, 2853, 3264
TaqII GACCGA 1 cut(s) 1712
TatI WGTACW 3 cut(s) 587, 902, 1956
TauI GCSGC 1 cut(s) 2538
TfiI GAWTC 4 cut(s) 103, 377, 1414, 3422
TscAI CASTG 4 cut(s) 219, 445, 1443, 3117
TseFI GTSAC 6 cut(s) 210, 340, 2143, 2884, 2894, 3211
Tsp45I GTSAC 6 cut(s) 210, 340, 2143, 2884, 2894, 3211
TspGWI ACGGA 1 cut(s) 1785
TspRI CASTG 4 cut(s) 219, 445, 1443, 3117
Van91I CCANNNNNTGG 1 cut(s) 257
Vha464I CTTAAG 1 cut(s) 737
VpaK11BI GGWCC 3 cut(s) 622, 1399, 2416
VspI ATTAAT 2 cut(s) 1131, 2814
XagI CCTNNNNNAGG 1 cut(s) 3053
XapI RAATTY 5 cut(s) 1105, 1382, 1888, 1907, 2731
XcmI CCANNNNNNNNNTGG 1 cut(s) 391
XmiI GTMKAC 4 cut(s) 581, 1328, 1861, 2171
XmnI GAANNNNTTC 2 cut(s) 29, 2611
XspI CTAG 7 cut(s) 18, 38, 366, 605, 747, 1086, 2180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.