Rw5G047180

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0012309
Physical Location & Seq
Forward (+)
81686797 .. 81691973
5177 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G047180.1

Sequence Viewer

Length: 3399 bp
ATGTTGCCTTCCAAGACTAAACAACTCTCCCAAATTTCCCACACAATTTCAAAGCTCAGAGCTTTTCATGGTGGTTCCTCTCCAAATGTCAAAAGCAATCTCAAAGAAAATGGCTTCAGAAACAGAGCTCATAGAACCACCCAAACCTCAGGTTCAGATATGGAGAAAAGCATATACTCAATTCTCACTATAGACCGATGGGAGTCACTGAACCACATGGAGTATAAGTTGGCTTCTCTTAGACCAGTGCATGGAAGATTGGCCTTGAAATTCTTCAACTGGGTCATTGAACAGCCTGGTTTGGAACTCAATCATCTCACTCATATACTCTCTGTTACAACTCATATACTTGCTAGAGCTAGAATGTATGACTCTGCCAGGTTAATTTTGGGGCATTTAGCACAATTGGGTGCTGACTCGAAATCTGTTTTTGGTGCTTTGATGGATACTTACCCTCTTTGCAATTCAAGCCCTTCAGTATTTGACCTTTTGATTAGAGTTTACTTGAGGGAAGGGATGGTTGGTAATGCTGTAGAGACTTTTTATTTGATGGGTTTAAGGGGGTTTAGGCCGTCGGGGTATACTTGTAATATGATTCTAGCTTCGTTGGCAAAGGACCAGAGGGTTGCATCGGTGTGGTCTTTTTTCAAAGAAATGCTGGCTAATAATGTTTGCCCTGATGTTAGTACTTTTAATATATTGATTAGTGTTTTGTGTGTGGAAGGGAAGCTTAGTAAAGCTAGTTATCTTTTGAGGAAGATGGAAAAGAGTGGTTATGTTCCTAATGTAGTTACTTATAATACTTTGCTCAATTGGTACTGCAAAAAGGGGAGGTATAAAGCAGCTTTTGAGCTTATTGATCATATGGGTTCTAAGGGTATTGAAGCAGATGTATGTACATATAACATGCTTATTGAGGATTTGTGTAGAAACAGTAGTAGTGCGAAAGGTTATTTGCTGTTGAAGAAGATGAGAAGGAAGAAGTTATCTCCTAATCAGGTTACCTACAATATCCTTATTAATGGGTTTGTCAAGGAGGGGAAAATTGGTGTGGCCACCAAGGTTTTTGATGAAATGTCAATGTTTAATCTTTCACCAAATTGTGTTACTTATAATGCTCTGATTGACGGGCTCTGTCAAAAGGGTAAGCTTGAAGAAGCTCTCAGACTCTTGGACATGATGGAAGCTGTGGGACTAAAACCTAATGAAGTCAGCTACGGTGCTGTTTTAAATGGGTTGTGCAAGCATGGTAAGTTTGATTTAGCAAGAAGTACTTTTCAGAGAATGCGATTGAATGGGATTGTTAGTGGTTGCATTATGTATACATCGATCATGGATGGGTTATGCAAAAATGGGTTGCTTGATGAATCTGTGCATTTGCTTAATATGATGGTCCAGGATGGTGTGAATCCGGATATAGTAGCATTTTCAGTGCTCATAACTGGATTCTGCAGAGCTGGGAGGATGAAATATGTAAGAGAAATAATGTGTAAACTGTATAGAGCTGGCCTTGCACCGAATAAAATTATATATTCTACCTTAATCTACTACTCTTGCAAGATGGGCAACATAATCGAAGCATTAAAGATCTATGTAGTTATGAATCATAATGGCCACACTGCCGACCTTTTCACATGTAACATATTGGTTACTGCACTTTGTGAAGCCGGGTACCTTGGTGCTGCAGAGGATTTCATGCAGCATATGAGCAGCATGGGTTTGGTTGCCAATTCCATTTCTTTTGATTGTATGATAAATGGGTATGGAAACATGGGCGATGCCTTAGGAGCGTTTTCTATGTTTGATGAAATGATTAGATTAGGTCATCACCCTAGTCCATTTACTTATGGAAGTATACTGAAAGGGCTATGCAGGGGTGGCCATTTGGAGGTGGCAAGGAAATTTTTGAAGAAACTCCACAGCATTCCATCGGCTGTTGATACTGTTGTGTACAATACAATACTTTCTGAGACGTGCAATTCTGGGAATTTGCAAGAGTCTGTTGTCCTTCTTGATGAGATGGTTGAGAATAATGTCCTGCCCGATACTCATACGTACAGTAGCCTTCTTGCTGGGTTATGCAGGAAGGGAAAAGTGGTTGCTGCCATCCTATTGTTTGGGAGAGTTATGGAAAAAGGAATTTTGTCACCAAATCCCATAATGTACACCTGTCTAGTTGATGGCCTTTTCAAGATTGGGCAACCTAAGGCTGCTTCATATCTATTTGAAGAGATGGAGAACAAAGGTCTGTACTCAGATACTATTGCTCTTAATGTAATGATGGATGGATACTCAAGGATGGGGAATATGATGAAGGCAAATGATCTCTTTTCAACTATGGGGGGCCGAAGATTGTCCCCCAATTTGGCTTCATGTAACATTCTGTTGCATGGATACTCTAAGAAACAAGACTTATTGGCATGCTCTATGGTGTACAAAAACATGATCAGGGTCAAATTATTGCCTGACAAATTGACATGTCACTCTCTTATTCTTGGATTATGTGAGTCAGGTAAGGTGGATATTGGCCACAAAATGTTGCATAAGATGATTATGGAAGGTGCTGTACCTGATCTTTTAACATTCAATTTGCTTATTAGCAAGTATTCTGAGATTGGGAAGATAGGGATGGCATTTGAGCTAGTGAGTGTCATGAATCTGTTAGGAGTTTCTGCTAATACTGACACTCATGGTGCAATCTTAAATGGACTGTTCAGAATTTCAGCTTTCCGCGCATCCCGTAGTCTTCTGTATGAAATGTTGGCCAAAGGATTTACTCCCAAGTATACACGTTATTTCACTATAATTAATGGTATGTGTAGAGTGGGAGATATACAAGGAGCATTTGAGCTAAAAGATCAGATGGAAGCTCTTGGTGTCACTTCCTGTGACATTGCTGAGAGTGCTATGGTTAGAGGGCTTGCGAAATGTGGGAAGGTTGAAGAAGCGATGCTGGTTCTTGATCGCATGCTTCGAATGCAACTTGTTCCAACTACTGCTACTTTTACTACTTTGATGCATAAGTTCTGCAAAGAAGCCAACCTTGCAGAGGCTCTAAGATTGAGGGGTGTAATGGAACATTGTGGTGTGCCACTCGATGTTGCTGCTTTTAATGTTCTCATTTCAGGCCTTTGTGCTAATGGTGATGTTGTGGCTGCATTTGAGCTGTACAAGGAGATGAAACGAAGTGGTCTCTTGCCCAACACAACAACGTACACTCTTCTCCTTGCTGCTGTTTCCTCTGGTAATAATCACATTGAAGGTGAAGAGATTTTGGGAGATTTATTGGGAAAGGGGTTGATATCTGGAAATTTGGATGGAAGCACCTTAACACTGCATGACCAATTGAATGTTGCAATGGGAACATTAAACTCTTGGAGGTGCAATAAGTGGAAATAA

Protein Analysis

1132

Amino Acids

125.19

Weight (kDa)

8.85

Isoelectric Point (pI)

31.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 195 - 239 5.3e-10 PPR repeat family
PPR_long PF17177 205 - 343 1.1e-11 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 216 - 274 3e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 223 - 255 1.9e-09 PPR repeat
PPR_2 PF13041 226 - 268 3.2e-11 PPR repeat family
TPR_24 PF23276 247 - 358 2.9e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 250 - 306 3.1e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 258 - 289 2.4e-11 PPR repeat
PPR_2 PF13041 261 - 310 7.6e-19 PPR repeat family
PPR PF01535 264 - 294 1.9e-07 PPR repeat
PPR_3 PF13812 285 - 345 5.5e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 297 - 345 1e-12 PPR repeat family
PPR_long PF17177 313 - 418 4e-09 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 328 - 360 1.8e-10 PPR repeat
PPR_2 PF13041 331 - 380 3.6e-17 PPR repeat family
PPR PF01535 334 - 361 4.3e-06 PPR repeat
PPR_3 PF13812 354 - 411 7e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 363 - 395 3.4e-14 PPR repeat
PPR_2 PF13041 366 - 415 8.8e-17 PPR repeat family
PPR PF01535 369 - 398 6.5e-09 PPR repeat
PPR_1 PF12854 398 - 430 1.4e-06 PPR repeat
PPR PF01535 404 - 434 7.1e-06 PPR repeat
PPR_1 PF12854 433 - 465 4.2e-06 PPR repeat
PPR_2 PF13041 439 - 485 8.3e-12 PPR repeat family
PPR PF01535 439 - 469 1.7e-06 PPR repeat
PPR_3 PF13812 460 - 515 9.5e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 467 - 490 1e-05 PPR repeat
PPR_2 PF13041 506 - 554 1.9e-07 PPR repeat family
PPR_2 PF13041 542 - 588 5.8e-07 PPR repeat family
TPR_24 PF23276 557 - 670 1.1e-06 Fungal tetratrico peptide repeats
PPR_2 PF13041 577 - 625 4.2e-11 PPR repeat family
PPR_2 PF13041 647 - 695 6.5e-14 PPR repeat family
PPR_1 PF12854 678 - 706 2.8e-06 PPR repeat
PPR_2 PF13041 753 - 801 2.6e-09 PPR repeat family
PPR_2 PF13041 787 - 835 5.8e-07 PPR repeat family
PPR_2 PF13041 857 - 905 3.3e-06 PPR repeat family
PPR_long PF17177 876 - 1013 6.7e-08 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 968 - 1060 2.3e-07 Fungal tetratrico peptide repeats
PPR_2 PF13041 969 - 1011 1.1e-07 PPR repeat family
PPR_2 PF13041 997 - 1045 3.5e-09 PPR repeat family
PPR_3 PF13812 1025 - 1079 2.2e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 1029 - 1061 1.5e-07 PPR repeat
PPR_2 PF13041 1033 - 1078 9.4e-15 PPR repeat family
PPR PF01535 1036 - 1065 1.2e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012309)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55840 AT5G55840 AT5G55840
fragaria_vesca FvH4_3g42930
malus_domestica MD03G1028500.v1.1
prunus_persica Prupe.6G024500_v2.0.a1 Prupe.6G024500_v2.0.a1
pyrus_communis pycom03g02310
rosa_chinensis RchiOBHm_Chr5g0076971
rosa_laevigata RLG00000036637
rosa_multiflora Rmu_sc0004655.1_g000020
rosa_roxburghii Rroxscaffold_1G00005180
rosa_rugosa Rorug05G0449800 Rorug05G0449900
rosa_samantha Rh5AG504500 Rh5BG526200 Rh5CG550100 Rh5DG538400
rosa_wichuraiana Rw5G047180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 798, 1113
Acc65I GGTACC 1 cut(s) 1671
AccB1I GGYRCC 1 cut(s) 1671
AccB7I CCANNNNNTGG 1 cut(s) 251
AccI GTMKAC 4 cut(s) 581, 1322, 1855, 2786
AccII CGCG 1 cut(s) 2733
AccIII TCCGGA 1 cut(s) 1411
AciI CCGC 1 cut(s) 2731
AcoI YGGCCR 5 cut(s) 1053, 1612, 1879, 2527, 2763
AcsI RAATTY 7 cut(s) 33, 269, 1901, 1987, 2139, 2718, 3310
AcuI CTGAAG 2 cut(s) 100, 459
AdeI CACNNNGTG 1 cut(s) 1661
AfiI CCNNNNNNNGG 4 cut(s) 251, 1888, 2365, 3049
AflIII ACRYGT 3 cut(s) 1634, 2477, 2789
AjiI CACGTC 1 cut(s) 1974
AjnI CCWGG 3 cut(s) 295, 377, 1395
AjuI GAANNNNNNNTTGG 2 cut(s) 504, 536
Alw21I GWGCWC 2 cut(s) 130, 1437
Alw26I GTCTC 3 cut(s) 530, 1964, 3197
Aor13HI TCCGGA 1 cut(s) 1411
ApeKI GCWGC 9 cut(s) 842, 1682, 1699, 1710, 2102, 2210, 3104, 3155, 3230
ApoI RAATTY 7 cut(s) 33, 269, 1901, 1987, 2139, 2718, 3310
ArsI GACNNNNNNTTYG 2 cut(s) 529, 561
AseI ATTAAT 2 cut(s) 1020, 2808
Asp700I GAANNNNTTC 1 cut(s) 272
Asp718I GGTACC 1 cut(s) 1671
AspLEI GCGC 1 cut(s) 2735
AspS9I GGNCC 3 cut(s) 616, 1393, 2344
AsuC2I CCSGG 1 cut(s) 1669
AsuHPI GGTGA 5 cut(s) 1086, 1820, 2139, 3155, 3275
AsuII TTCGAA 1 cut(s) 2974
AvaII GGWCC 2 cut(s) 616, 1393
AxyI CCTNAGG 3 cut(s) 148, 1783, 2205
BaeI ACNNNNGTAYC 4 cut(s) 1663, 1696, 1932, 1965
BalI TGGCCA 5 cut(s) 1055, 1614, 1881, 2529, 2765
BanI GGYRCC 1 cut(s) 1671
BanII GRGCYC 2 cut(s) 130, 1134
BarI GAAGNNNNNNTAC 2 cut(s) 2550, 2582
BbsI GAAGAC 1 cut(s) 2738
Bbv12I GWGCWC 2 cut(s) 130, 1437
BbvI GCAGC 9 cut(s) 854, 1669, 1711, 1722, 2089, 2197, 3091, 3142, 3217
BceAI ACGGC 1 cut(s) 556
BcgI CGANNNNNNTGC 4 cut(s) 1555, 1589, 3086, 3120
BciT130I CCWGG 3 cut(s) 297, 379, 1397
BciVI GTATCC 3 cut(s) 439, 2282, 2387
BclI TGATCA 2 cut(s) 859, 2445
BcnI CCSGG 1 cut(s) 1669
BcoDI GTCTC 3 cut(s) 530, 1964, 3197
BfaI CTAG 7 cut(s) 354, 360, 599, 741, 1833, 2174, 2642
BfmI CTRYAG 4 cut(s) 189, 531, 1450, 1683
BfuI GTATCC 3 cut(s) 439, 2282, 2387
BglII AGATCT 1 cut(s) 1587
BisI GCNGC 9 cut(s) 843, 1683, 1700, 1711, 2103, 2211, 3105, 3156, 3231
BlsI GCNGC 9 cut(s) 844, 1684, 1701, 1712, 2104, 2212, 3106, 3157, 3232
BmcAI AGTACT 2 cut(s) 688, 1273
Bme1390I CCNGG 4 cut(s) 297, 379, 1397, 1669
Bme18I GGWCC 2 cut(s) 616, 1393
BmgBI CACGTC 1 cut(s) 1974
BmgT120I GGNCC 3 cut(s) 616, 1393, 2344
BmiI GGNNCC 3 cut(s) 76, 1673, 2345
BmrFI CCNGG 4 cut(s) 297, 379, 1397, 1669
BmrI ACTGGG 1 cut(s) 289
BmsI GCATC 5 cut(s) 638, 1768, 2744, 2940, 3006
BmuI ACTGGG 1 cut(s) 289
BpiI GAAGAC 1 cut(s) 2738
Bpu14I TTCGAA 1 cut(s) 2974
BpuEI CTTGAG 2 cut(s) 526, 2278
BpuMI CCSGG 1 cut(s) 1669
Bsa29I ATCGAT 1 cut(s) 1328
BsaAI YACGTR 1 cut(s) 2055
BsaI GGTCTC 1 cut(s) 3197
BsaJI CCNNGG 2 cut(s) 1059, 1675
BsaWI WCCGGW 1 cut(s) 1411
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bsc4I CCNNNNNNNGG 4 cut(s) 251, 1888, 2365, 3049
Bse1I ACTGG 3 cut(s) 245, 284, 1447
Bse21I CCTNAGG 3 cut(s) 148, 1783, 2205
Bse3DI GCAATG 2 cut(s) 2892, 3363
BseAI TCCGGA 1 cut(s) 1411
BseBI CCWGG 3 cut(s) 297, 379, 1397
BseCI ATCGAT 1 cut(s) 1328
BseDI CCNNGG 2 cut(s) 1059, 1675
BseLI CCNNNNNNNGG 4 cut(s) 251, 1888, 2365, 3049
BseMI GCAATG 2 cut(s) 2892, 3363
BseMII CTCAG 7 cut(s) 70, 162, 1177, 1959, 2268, 2601, 2889
BseNI ACTGG 3 cut(s) 245, 284, 1447
BseXI GCAGC 9 cut(s) 854, 1669, 1711, 1722, 2089, 2197, 3091, 3142, 3217
BseYI CCCAGC 2 cut(s) 1457, 2072
BsgI GTGCAG 1 cut(s) 1638
Bsh1236I CGCG 1 cut(s) 2733
BshNI GGYRCC 1 cut(s) 1671
BshVI ATCGAT 1 cut(s) 1328
BsiHKAI GWGCWC 2 cut(s) 130, 1437
BsiSI CCGG 2 cut(s) 1412, 1668
BslFI GGGAC 2 cut(s) 1206, 2341
BslI CCNNNNNNNGG 4 cut(s) 251, 1888, 2365, 3049
BsmAI GTCTC 3 cut(s) 530, 1964, 3197
BsmBI CGTCTC 1 cut(s) 1964
BsmFI GGGAC 2 cut(s) 1206, 2341
BsmI GAATGC 3 cut(s) 1290, 1923, 2982
Bso31I GGTCTC 1 cut(s) 3197
Bsp119I TTCGAA 1 cut(s) 2974
Bsp1286I GDGCHC 3 cut(s) 130, 1134, 1437
Bsp13I TCCGGA 1 cut(s) 1411
Bsp1407I TGTACA 5 cut(s) 896, 1950, 2163, 2433, 3168
Bsp143I GATC 8 cut(s) 859, 1329, 1587, 2323, 2445, 2572, 2857, 2962
BspACI CCGC 1 cut(s) 2731
BspCNI CTCAG 7 cut(s) 69, 161, 1176, 1960, 2267, 2602, 2890
BspDI ATCGAT 1 cut(s) 1328
BspEI TCCGGA 1 cut(s) 1411
BspFNI CGCG 1 cut(s) 2733
BspHI TCATGA 1 cut(s) 2652
BspLI GGNNCC 3 cut(s) 76, 1673, 2345
BspMAI CTGCAG 2 cut(s) 1454, 1687
BspT104I TTCGAA 1 cut(s) 2974
BspT107I GGYRCC 1 cut(s) 1671
BspTNI GGTCTC 1 cut(s) 3197
BsrDI GCAATG 2 cut(s) 2892, 3363
BsrGI TGTACA 5 cut(s) 896, 1950, 2163, 2433, 3168
BsrI ACTGG 3 cut(s) 245, 284, 1447
BssECI CCNNGG 2 cut(s) 1059, 1675
BssMI GATC 8 cut(s) 859, 1329, 1587, 2323, 2445, 2572, 2857, 2962
BssNAI GTATAC 4 cut(s) 582, 1323, 1856, 2787
BssT1I CCWWGG 2 cut(s) 1059, 1675
Bst1107I GTATAC 4 cut(s) 582, 1323, 1856, 2787
Bst2UI CCWGG 3 cut(s) 297, 379, 1397
Bst4CI ACNGT 6 cut(s) 935, 1220, 1497, 1945, 2060, 2712
Bst6I CTCTTC 3 cut(s) 2223, 3225, 3261
BstAUI TGTACA 5 cut(s) 896, 1950, 2163, 2433, 3168
BstBAI YACGTR 1 cut(s) 2055
BstBI TTCGAA 1 cut(s) 2974
BstC8I GCNNGC 6 cut(s) 660, 1244, 1507, 2422, 2922, 2969
BstEII GGTNACC 1 cut(s) 1000
BstENI CCTNNNNNAGG 1 cut(s) 3047
BstFNI CGCG 1 cut(s) 2733
BstHHI GCGC 1 cut(s) 2735
BstKTI GATC 8 cut(s) 862, 1332, 1590, 2326, 2448, 2575, 2860, 2965
BstMAI GTCTC 3 cut(s) 530, 1964, 3197
BstMBI GATC 8 cut(s) 859, 1329, 1587, 2323, 2445, 2572, 2857, 2962
BstNI CCWGG 3 cut(s) 297, 379, 1397
BstNSI RCATGY 5 cut(s) 910, 1638, 2424, 2481, 2971
BstPI GGTNACC 1 cut(s) 1000
BstSCI CCNGG 4 cut(s) 295, 377, 1395, 1667
BstSFI CTRYAG 4 cut(s) 189, 531, 1450, 1683
BstSNI TACGTA 1 cut(s) 2055
BstUI CGCG 1 cut(s) 2733
BstV1I GCAGC 9 cut(s) 854, 1669, 1711, 1722, 2089, 2197, 3091, 3142, 3217
BstV2I GAAGAC 1 cut(s) 2738
BstX2I RGATCY 1 cut(s) 1587
BstYI RGATCY 1 cut(s) 1587
BstZ17I GTATAC 4 cut(s) 582, 1323, 1856, 2787
Bsu15I ATCGAT 1 cut(s) 1328
Bsu36I CCTNAGG 3 cut(s) 148, 1783, 2205
BsuI GTATCC 3 cut(s) 439, 2282, 2387
BsuTUI ATCGAT 1 cut(s) 1328
BtgZI GCGATG 2 cut(s) 1791, 2963
BtrI CACGTC 1 cut(s) 1974
BtsI GCAGTG 2 cut(s) 1617, 3332
BtsIMutI CAGTG 5 cut(s) 206, 252, 1437, 1617, 3332
Cac8I GCNNGC 6 cut(s) 660, 1244, 1507, 2422, 2922, 2969
CciI TCATGA 1 cut(s) 2652
CfoI GCGC 1 cut(s) 2735
Cfr13I GGNCC 3 cut(s) 616, 1393, 2344
ClaI ATCGAT 1 cut(s) 1328
DpnI GATC 8 cut(s) 861, 1331, 1589, 2325, 2447, 2574, 2859, 2964
DpnII GATC 8 cut(s) 859, 1329, 1587, 2323, 2445, 2572, 2857, 2962
DraI TTTAAA 1 cut(s) 1230
DraIII CACNNNGTG 1 cut(s) 1661
EaeI YGGCCR 5 cut(s) 1053, 1612, 1879, 2527, 2763
Eam1104I CTCTTC 3 cut(s) 2223, 3225, 3261
EarI CTCTTC 3 cut(s) 2223, 3225, 3261
Ecl136II GAGCTC 1 cut(s) 128
Eco105I TACGTA 1 cut(s) 2055
Eco130I CCWWGG 2 cut(s) 1059, 1675
Eco147I AGGCCT 1 cut(s) 3129
Eco24I GRGCYC 2 cut(s) 130, 1134
Eco31I GGTCTC 1 cut(s) 3197
Eco32I GATATC 1 cut(s) 3303
Eco47I GGWCC 2 cut(s) 616, 1393
Eco53kI GAGCTC 1 cut(s) 128
Eco57I CTGAAG 2 cut(s) 100, 459
Eco81I CCTNAGG 3 cut(s) 148, 1783, 2205
Eco91I GGTNACC 1 cut(s) 1000
EcoICRI GAGCTC 1 cut(s) 128
EcoNI CCTNNNNNAGG 1 cut(s) 3047
EcoO65I GGTNACC 1 cut(s) 1000
EcoRII CCWGG 3 cut(s) 295, 377, 1395
EcoRV GATATC 1 cut(s) 3303
EcoT14I CCWWGG 2 cut(s) 1059, 1675
EcoT22I ATGCAT 1 cut(s) 3021
EcoT38I GRGCYC 2 cut(s) 130, 1134
ErhI CCWWGG 2 cut(s) 1059, 1675
Esp3I CGTCTC 1 cut(s) 1964
FalI AAGNNNNNCTT 6 cut(s) 714, 746, 1258, 1290, 3027, 3059
FaqI GGGAC 2 cut(s) 1206, 2341
FauNDI CATATG 2 cut(s) 864, 1704
FbaI TGATCA 2 cut(s) 859, 2445
FblI GTMKAC 4 cut(s) 581, 1322, 1855, 2786
Fnu4HI GCNGC 9 cut(s) 843, 1683, 1700, 1711, 2103, 2211, 3105, 3156, 3231
FriOI GRGCYC 2 cut(s) 130, 1134
Fsp4HI GCNGC 9 cut(s) 843, 1683, 1700, 1711, 2103, 2211, 3105, 3156, 3231
FspBI CTAG 7 cut(s) 354, 360, 599, 741, 1833, 2174, 2642
GlaI GCGC 1 cut(s) 2734
GluI GCNGC 9 cut(s) 843, 1683, 1700, 1711, 2103, 2211, 3105, 3156, 3231
GsaI CCCAGC 2 cut(s) 1461, 2076
HapII CCGG 2 cut(s) 1412, 1668
HhaI GCGC 1 cut(s) 2735
Hin6I GCGC 1 cut(s) 2733
HinP1I GCGC 1 cut(s) 2733
HindIII AAGCTT 2 cut(s) 728, 1148
HpaII CCGG 2 cut(s) 1412, 1668
HphI GGTGA 5 cut(s) 1086, 1820, 2139, 3155, 3275
Hpy188III TCNNGA 6 cut(s) 1412, 2012, 2191, 2653, 2960, 3306
Hpy99I CGWCG 1 cut(s) 577
HpyCH4III ACNGT 6 cut(s) 935, 1220, 1497, 1945, 2060, 2712
HpyCH4IV ACGT 4 cut(s) 1973, 2054, 2791, 3212
HpySE526I ACGT 4 cut(s) 1973, 2054, 2791, 3212
HspAI GCGC 1 cut(s) 2733
Kpn2I TCCGGA 1 cut(s) 1411
KpnI GGTACC 1 cut(s) 1675
Ksp22I TGATCA 2 cut(s) 859, 2445
Kzo9I GATC 8 cut(s) 859, 1329, 1587, 2323, 2445, 2572, 2857, 2962
LmnI GCTCC 2 cut(s) 1787, 2840
Lsp1109I GCAGC 9 cut(s) 854, 1669, 1711, 1722, 2089, 2197, 3091, 3142, 3217
LweI GCATC 5 cut(s) 638, 1768, 2744, 2940, 3006
MaeI CTAG 7 cut(s) 354, 360, 599, 741, 1833, 2174, 2642
MaeII ACGT 4 cut(s) 1973, 2054, 2791, 3212
MalI GATC 8 cut(s) 861, 1331, 1589, 2325, 2447, 2574, 2859, 2964
MboI GATC 8 cut(s) 859, 1329, 1587, 2323, 2445, 2572, 2857, 2962
MfeI CAATTG 3 cut(s) 404, 811, 3344
MflI RGATCY 1 cut(s) 1587
MhlI GDGCHC 3 cut(s) 130, 1134, 1437
MlsI TGGCCA 5 cut(s) 1055, 1614, 1881, 2529, 2765
MluNI TGGCCA 5 cut(s) 1055, 1614, 1881, 2529, 2765
MlyI GAGTC 6 cut(s) 212, 365, 410, 1161, 2006, 2516
MmeI TCCRAC 1 cut(s) 3014
Mox20I TGGCCA 5 cut(s) 1055, 1614, 1881, 2529, 2765
Mph1103I ATGCAT 1 cut(s) 3021
MroI TCCGGA 1 cut(s) 1411
MroXI GAANNNNTTC 1 cut(s) 272
MscI TGGCCA 5 cut(s) 1055, 1614, 1881, 2529, 2765
MslI CAYNNNNRTG 2 cut(s) 634, 3084
Msp20I TGGCCA 5 cut(s) 1055, 1614, 1881, 2529, 2765
MspI CCGG 2 cut(s) 1412, 1668
MspR9I CCNGG 4 cut(s) 297, 379, 1397, 1669
MunI CAATTG 3 cut(s) 404, 811, 3344
Mva1269I GAATGC 3 cut(s) 1290, 1923, 2982
MvaI CCWGG 3 cut(s) 297, 379, 1397
MvnI CGCG 1 cut(s) 2733
NciI CCSGG 1 cut(s) 1669
NdeI CATATG 2 cut(s) 864, 1704
NdeII GATC 8 cut(s) 859, 1329, 1587, 2323, 2445, 2572, 2857, 2962
NlaIV GGNNCC 3 cut(s) 76, 1673, 2345
NmuCI GTSAC 5 cut(s) 204, 2145, 2480, 2878, 2888
NsiI ATGCAT 1 cut(s) 3021
NspI RCATGY 5 cut(s) 910, 1638, 2424, 2481, 2971
NspV TTCGAA 1 cut(s) 2974
PaeI GCATGC 2 cut(s) 2424, 2971
PagI TCATGA 1 cut(s) 2652
PceI AGGCCT 1 cut(s) 3129
PciI ACATGT 2 cut(s) 1634, 2477
PcsI WCGNNNNNNNCGW 1 cut(s) 2971
PctI GAATGC 3 cut(s) 1290, 1923, 2982
PdmI GAANNNNTTC 1 cut(s) 272
PfeI GAWTC 6 cut(s) 595, 1367, 1408, 1446, 1603, 2656
PflMI CCANNNNNTGG 1 cut(s) 251
PfoI TCCNGGA 1 cut(s) 1395
PkrI GCNGC 9 cut(s) 844, 1684, 1701, 1712, 2104, 2212, 3106, 3157, 3232
PleI GAGTC 6 cut(s) 211, 365, 410, 1161, 2005, 2515
PpsI GAGTC 6 cut(s) 211, 365, 410, 1161, 2005, 2515
Ppu21I YACGTR 1 cut(s) 2055
PscI ACATGT 2 cut(s) 1634, 2477
PshBI ATTAAT 2 cut(s) 1020, 2808
PsiI TTATAA 2 cut(s) 798, 1113
Psp124BI GAGCTC 1 cut(s) 130
Psp6I CCWGG 3 cut(s) 295, 377, 1395
PspEI GGTNACC 1 cut(s) 1000
PspFI CCCAGC 2 cut(s) 1457, 2072
PspGI CCWGG 3 cut(s) 295, 377, 1395
PspN4I GGNNCC 3 cut(s) 76, 1673, 2345
PspPI GGNCC 3 cut(s) 616, 1393, 2344
PstI CTGCAG 2 cut(s) 1454, 1687
PsuI RGATCY 1 cut(s) 1587
RseI CAYNNNNRTG 2 cut(s) 634, 3084
SacI GAGCTC 1 cut(s) 130
SatI GCNGC 9 cut(s) 843, 1683, 1700, 1711, 2103, 2211, 3105, 3156, 3231
Sau3AI GATC 8 cut(s) 859, 1329, 1587, 2323, 2445, 2572, 2857, 2962
Sau96I GGNCC 3 cut(s) 616, 1393, 2344
ScaI AGTACT 2 cut(s) 688, 1273
SchI GAGTC 6 cut(s) 212, 365, 410, 1161, 2006, 2516
ScrFI CCNGG 4 cut(s) 297, 379, 1397, 1669
SduI GDGCHC 3 cut(s) 130, 1134, 1437
SfaNI GCATC 5 cut(s) 638, 1768, 2744, 2940, 3006
SfcI CTRYAG 4 cut(s) 189, 531, 1450, 1683
SfuI TTCGAA 1 cut(s) 2974
SinI GGWCC 2 cut(s) 616, 1393
SmiMI CAYNNNNRTG 2 cut(s) 634, 3084
SmlI CTYRAG 2 cut(s) 505, 2293
SmoI CTYRAG 2 cut(s) 505, 2293
SnaBI TACGTA 1 cut(s) 2055
SphI GCATGC 2 cut(s) 2424, 2971
SseBI AGGCCT 1 cut(s) 3129
SsiI CCGC 1 cut(s) 2731
SspMI CTAG 7 cut(s) 354, 360, 599, 741, 1833, 2174, 2642
SstI GAGCTC 1 cut(s) 130
StuI AGGCCT 1 cut(s) 3129
StyD4I CCNGG 4 cut(s) 295, 377, 1395, 1667
StyI CCWWGG 2 cut(s) 1059, 1675
TaaI ACNGT 6 cut(s) 935, 1220, 1497, 1945, 2060, 2712
TaiI ACGT 4 cut(s) 1976, 2057, 2794, 3215
TaqI TCGA 5 cut(s) 419, 1328, 1575, 2974, 3096
TaqII GACCGA 1 cut(s) 210
TatI WGTACW 8 cut(s) 686, 896, 1271, 1950, 2163, 2250, 2433, 3168
TfiI GAWTC 6 cut(s) 595, 1367, 1408, 1446, 1603, 2656
TscAI CASTG 5 cut(s) 213, 252, 1437, 1624, 3339
TseFI GTSAC 5 cut(s) 204, 2145, 2480, 2878, 2888
TseI GCWGC 9 cut(s) 842, 1682, 1699, 1710, 2102, 2210, 3104, 3155, 3230
Tsp45I GTSAC 5 cut(s) 204, 2145, 2480, 2878, 2888
TspRI CASTG 5 cut(s) 213, 252, 1437, 1624, 3339
Van91I CCANNNNNTGG 1 cut(s) 251
VpaK11BI GGWCC 2 cut(s) 616, 1393
VspI ATTAAT 2 cut(s) 1020, 2808
XagI CCTNNNNNAGG 1 cut(s) 3047
XapI RAATTY 7 cut(s) 33, 269, 1901, 1987, 2139, 2718, 3310
XceI RCATGY 5 cut(s) 910, 1638, 2424, 2481, 2971
XcmI CCANNNNNNNNNTGG 1 cut(s) 385
XmiI GTMKAC 4 cut(s) 581, 1322, 1855, 2786
XmnI GAANNNNTTC 1 cut(s) 272
XspI CTAG 7 cut(s) 354, 360, 599, 741, 1833, 2174, 2642
ZrmI AGTACT 2 cut(s) 688, 1273
Zsp2I ATGCAT 1 cut(s) 3021
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.