Rroxscaffold_1G00005180

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0012309
Physical Location & Seq
Forward (+)
7033124 .. 7038345
5222 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00005180.1

Sequence Viewer

Length: 3399 bp
ATGTCGCCTTCCAAGACTAAACAACTCTCCCAAATTTCCCACACAATTTCAAAGCTCAGAGCTTTTCATGGTGGTTCCTCTCCGAATGTCAAAAGCAATCTCAAAGAAAATGGCTTCAGAAACAGAGCTCATAGAACCACCCAAACCTCAGGTTCAGATATGGAGAAAAGCATATACTCAATTCTCACTATAGACCGATGGGAGTCACTGAACCACATGGAGTATAAGTTGGCTTCTCTTAGACCAGTGCATGGAAGATTGGCCTTGAAATTCTTCAACTGGGTCATTGAACAGCCTGGTTTGGAACTCAATCATCTAACTCATATACTCTCTGTTACAACTCATATACTTGCTAGAGCTAGAATGTATGACTCTGCCAGGTTAATTTTGGGGCATTTAGCACAATTGGGTGCTGACTCAAAATCTATTTTTGGTGCTTTGATGGATACTTACCCTCTTTGCAATTCAAACCCTTCAGTATTTGACCTTTTGATTAGAGTTTACTTGAGGGAAGGGATGGTTGGTAATGCTGTAGAGACTTTTTATTTGATGGGTTTAAGGGGGTTTAGGCCGTCGGGTTATACTTGTAATATGATTCTAGCTTTGTTGGCAAAGGACCAGAGGGCTGCATCAGTATGGTCTTTTTTCAAAGAAATGCTGGCTAAGAATGTTTGCCCTGATGTTTCTACTTTTAATATATTGATTAGTGTTTTATGCGTGGAAGGGAAGCTTAGTAAAGCCAGTTATCTTTTGAGGAAGATGGAAAAGAGTGGTTATGTTCCTAATGTAGTTACTTATAATACTTTGCTCAATTGGTACTGCAAGAAGGGGAGGTATAAAGCAGCTTTTGAGCTTATTGATCATATGGGTTCTAAGGGTATTGAAGCCGATGTATGTACATATAACATGCTTATTGAGGATTTGTGTAGAAACAGTAGTAGTGCGAAAGGTTACTTGCTGTTGAAGAAGATGAGAAGGAAGAAGTTATATCCTAATCAGGTTACCTACAATATCCTTATTAATGGGTTTGTCAAGGAGGGGAAAATTGGTGTGGCCACCAAGGTTTTTGATGAAATGTCAATGTTTAATCTTTCACCAAATTGTGTTACTTATAATGCTCTGATTGACGGGCTCTGTCAAAAGGGTAAGCTTGAAGAAGCTCTCAGACTCTTGGACATGATGGAAGCAGTGGGACTAAAACCTAATGAAGTCAGCTACGGTGCTGTTTTAAATGGGTTGTGCAAGCATGGTAAGTTTGATTTAGCAAGAAGTACTTTTCAGAGAATGCGATTGAATGGGATTGTTAGTGGTTGCATTATGTATACATCGATCATGGATGGGTTATGCAAAAATGGGTTGCTTGATGAATCTGTGCATTTGCTTAATATGATGGTCCAGGATGGTGTGAATCCTGATATCGTAGCATTTTCAGTGCTCATAGATGGATTCTGCAGAGCTGGGAGGATGAAACATGTAAGAGAAATAATGTGTAAACTGTATAGAGCTGGCCTTGCACCGAATAAAATTATATATTCTACCTTAATCTACTACTCTTGCAAGATGGGCAACATAATCGAAGCATTAAAGATCTATGTAGTTATGAATCATAATGGCCACACTGCTGACCATTTCACATGTAACATATTGGTTACTGCACTTTGTGAAGCTGGGAACCTTGGTGCTGCAGAGGATTTCATGCAGCATATGAGCAGCATGGGTCTGGTTGCCAATTCCATTTCTTTTGATTGTATGATAAATGGGTATGGAAACATGGGTGATGCCTTAGGAGCGTTTTCTATGTTTGATGAAATGATAAGATTAGGTCATCACCCTAGTCCATTTACTTATGGAAGTATACTAAAAGGGCTATGCAGGGGTGGTCATTTGGAGGTGGCGAGGAAATTTTTGAAGAAACTCCACAGCATTCCGTCGGCTGTAGATACTGTTATGTACAATACAATACTTTCTGAGACATGCAATTCTGGGAATTTGCAAGAGGCTGTTGTCCTTCTTGATGAGATGGTTGAGAATAATGTCCTGCCTGATACTCATACGTACAGTAGCCTTCTTGCTGGGTTATGCAGGAAGGGAAAAGTGGTTGCTGCCATTCTATTGTTTGGGAGAGTTATGGAAAAAGGAATTTTGTCACCAAATCCCATAATGTACACCTGTCTAGTTGATGGCCTTTTCAAGATTGGGCAACCTAAGGCTGCTTCATATCTATTTGAAGAGATGGAGAACAAAGGTCTGTACTCGGATACTACTGCTCTTAATGTAATGATGGATGGATACTCAAGGATGGGAAAAATGATGAAGGCAAATGATCTCTTTTCAACTATGGGTGGCCGAAGATTGTCCCCCAACTTGGCTTCATGTAACATTCTGTTGCATGGATACTCAAAGAAACAAGACTTCTTGGCATGCTCTATGGTGTACAAAAACATGATCAGGGTCAAATTATTGCCTGACAAATTGACATGTCACTCTCTTATTCTTGGATTATGTGAGTCAGGTAAGGTGGATATTGGCCACAAAATGTTGCATAAGATGATTATGGAAGGTGCTGTACCTGATCTTTTAACATTCAATTTGCTTATTAGCAAGTTTTCTGAGATTGGGAAGATAGGGATGGCATATGAGCTAGTGAGTGTCATGAATCTGTTAGGAGTTTCTGCTAATACAGACACTCATGGCGCAATCTTAAATGGACTGTTCAGAATTTCAGCTTTCCGCGCATCCCGTAGTCTTCTGTATGACATGTTGGCCAAAGGATTTACTCCCAAGTATACACATTATTTCACTATAATTAATGGTATGTGTAGAGTGGGAGATATACAAGGAGCATTTGAGCTAAAAGATCAGATGGAAGCTCTTGGTGTCACTTCCTGTGACATTGCTGAGAGTGCTATGGTTAGAGGGCTTGCGAAATGTGGGAAGGTTGAAGAAGCGATGCTGGTTCTTGATCGCATGCTTCGAATGCAACTTGTTCCAACTACTGCTACTTTTACTACTTTGATGCATAAGTTCTGCAAAGAAGCCAACCTTGCAGAGGCTCTAAAATTGAGGGGTGTAATGGAACATTGTGGTGTGCCACTCGATGTTGCTGCTTTTAATGTTCTCATTTCAGGCCTTTGTGCTAATGGTGATGTTGTGGCTGCATTTCAGCTGTACAAGGAGATGAAACGAAGTGGTCTCTTGCCCAACACAACAACGTACACTCTTCTCCTTGCTGCTGTTTCCTCTGGTAATAATCATATTGAAGGTGAAGAGATTTTGGGAGATTTATTGGGAAAGGGTTTGATATCTGGAAATTTGGATGGAAGCACCTTAACACTGCATGAGCAATTGAATGTTGCAATGGGAACATTAAACTCTTGGAGGTGCAATAAATGGAAATAA

Protein Analysis

1132

Amino Acids

125.26

Weight (kDa)

8.87

Isoelectric Point (pI)

31.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 160 - 249 9.9e-06 Fungal tetratrico peptide repeats
PPR_long PF17177 188 - 343 4.2e-12 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 194 - 239 1.2e-09 PPR repeat family
PPR_3 PF13812 216 - 274 1.1e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 222 - 255 1.3e-09 PPR repeat
PPR_2 PF13041 226 - 270 2.2e-12 PPR repeat family
TPR_24 PF23276 247 - 358 4.9e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 250 - 306 3e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 258 - 289 2.4e-11 PPR repeat
PPR_2 PF13041 261 - 310 7.7e-19 PPR repeat family
PPR PF01535 264 - 294 1.9e-07 PPR repeat
PPR_3 PF13812 285 - 345 2.5e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 297 - 345 3.9e-12 PPR repeat family
PPR_long PF17177 312 - 418 1.5e-08 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 329 - 360 6e-10 PPR repeat
PPR_2 PF13041 331 - 380 3.6e-17 PPR repeat family
PPR PF01535 334 - 361 4.3e-06 PPR repeat
PPR_3 PF13812 354 - 411 7e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 363 - 395 3.4e-14 PPR repeat
PPR_2 PF13041 366 - 415 8.8e-17 PPR repeat family
PPR PF01535 369 - 398 6.5e-09 PPR repeat
PPR_1 PF12854 398 - 430 1.4e-06 PPR repeat
PPR PF01535 404 - 434 7.1e-06 PPR repeat
PPR_1 PF12854 433 - 465 4.2e-06 PPR repeat
PPR_2 PF13041 439 - 485 3.8e-12 PPR repeat family
PPR PF01535 439 - 469 1.7e-06 PPR repeat
PPR_1 PF12854 467 - 490 1.4e-06 PPR repeat
PPR_2 PF13041 471 - 515 9.3e-07 PPR repeat family
PPR_2 PF13041 506 - 554 7.5e-08 PPR repeat family
PPR_2 PF13041 542 - 589 1.2e-07 PPR repeat family
TPR_24 PF23276 562 - 670 2.9e-07 Fungal tetratrico peptide repeats
PPR_2 PF13041 577 - 625 4.2e-11 PPR repeat family
PPR_1 PF12854 647 - 675 3.6e-06 PPR repeat
PPR_2 PF13041 647 - 695 2.5e-15 PPR repeat family
PPR_1 PF12854 678 - 706 2.8e-06 PPR repeat
PPR_2 PF13041 753 - 801 3e-09 PPR repeat family
PPR_2 PF13041 787 - 835 5.1e-07 PPR repeat family
PPR_2 PF13041 822 - 869 7.1e-06 PPR repeat family
PPR_long PF17177 877 - 1013 2.8e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 969 - 1011 1.1e-07 PPR repeat family
TPR_24 PF23276 969 - 1060 2.9e-07 Fungal tetratrico peptide repeats
PPR_2 PF13041 997 - 1045 3e-09 PPR repeat family
PPR_3 PF13812 1025 - 1079 2.8e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 1029 - 1061 2e-07 PPR repeat
PPR_2 PF13041 1033 - 1078 9.2e-15 PPR repeat family
PPR PF01535 1036 - 1065 3e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012309)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55840 AT5G55840 AT5G55840
fragaria_vesca FvH4_3g42930
malus_domestica MD03G1028500.v1.1
prunus_persica Prupe.6G024500_v2.0.a1 Prupe.6G024500_v2.0.a1
pyrus_communis pycom03g02310
rosa_chinensis RchiOBHm_Chr5g0076971
rosa_laevigata RLG00000036637
rosa_multiflora Rmu_sc0004655.1_g000020
rosa_roxburghii Rroxscaffold_1G00005180
rosa_rugosa Rorug05G0449800 Rorug05G0449900
rosa_samantha Rh5AG504500 Rh5BG526200 Rh5CG550100 Rh5DG538400
rosa_wichuraiana Rw5G047180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 798, 1113
AccB7I CCANNNNNTGG 1 cut(s) 251
AccI GTMKAC 3 cut(s) 1322, 1855, 2786
AccII CGCG 1 cut(s) 2733
AciI CCGC 1 cut(s) 2731
AcoI YGGCCR 5 cut(s) 1053, 1612, 2344, 2527, 2763
AcsI RAATTY 7 cut(s) 33, 269, 1901, 1987, 2139, 2718, 3310
AcuI CTGAAG 2 cut(s) 100, 459
AdeI CACNNNGTG 1 cut(s) 1661
AfiI CCNNNNNNNGG 3 cut(s) 251, 2365, 3049
AflIII ACRYGT 4 cut(s) 1471, 1634, 2477, 2757
AjnI CCWGG 3 cut(s) 295, 377, 1395
AjuI GAANNNNNNNTTGG 2 cut(s) 504, 536
Alw21I GWGCWC 2 cut(s) 130, 1437
Alw26I GTCTC 3 cut(s) 530, 1964, 3197
ApoI RAATTY 7 cut(s) 33, 269, 1901, 1987, 2139, 2718, 3310
ArsI GACNNNNNNTTYG 2 cut(s) 529, 561
AseI ATTAAT 2 cut(s) 1020, 2808
Asp700I GAANNNNTTC 1 cut(s) 272
AspLEI GCGC 2 cut(s) 2696, 2735
AspS9I GGNCC 2 cut(s) 616, 1393
AsuHPI GGTGA 6 cut(s) 1086, 1787, 1820, 2139, 3155, 3275
AsuII TTCGAA 1 cut(s) 2974
AvaII GGWCC 2 cut(s) 616, 1393
AxyI CCTNAGG 3 cut(s) 148, 1783, 2205
BalI TGGCCA 4 cut(s) 1055, 1614, 2529, 2765
BanII GRGCYC 2 cut(s) 130, 1134
BarI GAAGNNNNNNTAC 2 cut(s) 2550, 2582
BbsI GAAGAC 1 cut(s) 2738
Bbv12I GWGCWC 2 cut(s) 130, 1437
BceAI ACGGC 1 cut(s) 556
BcgI CGANNNNNNTGC 4 cut(s) 1555, 1589, 3086, 3120
BciT130I CCWGG 3 cut(s) 297, 379, 1397
BciVI GTATCC 4 cut(s) 439, 2251, 2282, 2387
BclI TGATCA 2 cut(s) 859, 2445
BcoDI GTCTC 3 cut(s) 530, 1964, 3197
BfaI CTAG 6 cut(s) 354, 360, 599, 1833, 2174, 2642
BfmI CTRYAG 5 cut(s) 189, 531, 1450, 1683, 1935
BfuI GTATCC 4 cut(s) 439, 2251, 2282, 2387
BglII AGATCT 1 cut(s) 1587
BmcAI AGTACT 1 cut(s) 1273
Bme1390I CCNGG 3 cut(s) 297, 379, 1397
Bme18I GGWCC 2 cut(s) 616, 1393
BmgT120I GGNCC 2 cut(s) 616, 1393
BmiI GGNNCC 2 cut(s) 76, 1673
BmrFI CCNGG 3 cut(s) 297, 379, 1397
BmrI ACTGGG 1 cut(s) 289
BmsI GCATC 5 cut(s) 638, 1768, 2744, 2940, 3006
BmuI ACTGGG 1 cut(s) 289
BpiI GAAGAC 1 cut(s) 2738
Bpu14I TTCGAA 1 cut(s) 2974
BpuEI CTTGAG 2 cut(s) 526, 2278
Bsa29I ATCGAT 1 cut(s) 1328
BsaAI YACGTR 1 cut(s) 2055
BsaI GGTCTC 1 cut(s) 3197
BsaJI CCNNGG 2 cut(s) 1059, 1675
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bsc4I CCNNNNNNNGG 3 cut(s) 251, 2365, 3049
Bse1I ACTGG 3 cut(s) 245, 284, 741
Bse21I CCTNAGG 3 cut(s) 148, 1783, 2205
Bse3DI GCAATG 2 cut(s) 2892, 3363
BseBI CCWGG 3 cut(s) 297, 379, 1397
BseCI ATCGAT 1 cut(s) 1328
BseDI CCNNGG 2 cut(s) 1059, 1675
BseGI GGATG 9 cut(s) 522, 1342, 1405, 1470, 2290, 2304, 2634, 2735, 3322
BseLI CCNNNNNNNGG 3 cut(s) 251, 2365, 3049
BseMI GCAATG 2 cut(s) 2892, 3363
BseMII CTCAG 6 cut(s) 70, 162, 1177, 1959, 2601, 2889
BseNI ACTGG 3 cut(s) 245, 284, 741
BseYI CCCAGC 3 cut(s) 1457, 1667, 2072
BsgI GTGCAG 1 cut(s) 1638
Bsh1236I CGCG 1 cut(s) 2733
BshVI ATCGAT 1 cut(s) 1328
BsiHKAI GWGCWC 2 cut(s) 130, 1437
BslFI GGGAC 2 cut(s) 1206, 2341
BslI CCNNNNNNNGG 3 cut(s) 251, 2365, 3049
BsmAI GTCTC 3 cut(s) 530, 1964, 3197
BsmFI GGGAC 2 cut(s) 1206, 2341
BsmI GAATGC 3 cut(s) 1290, 1923, 2982
Bso31I GGTCTC 1 cut(s) 3197
Bsp119I TTCGAA 1 cut(s) 2974
Bsp1286I GDGCHC 3 cut(s) 130, 1134, 1437
Bsp1407I TGTACA 5 cut(s) 896, 1950, 2163, 2433, 3168
Bsp143I GATC 8 cut(s) 859, 1329, 1587, 2323, 2445, 2572, 2857, 2962
BspACI CCGC 1 cut(s) 2731
BspCNI CTCAG 6 cut(s) 69, 161, 1176, 1960, 2602, 2890
BspDI ATCGAT 1 cut(s) 1328
BspFNI CGCG 1 cut(s) 2733
BspHI TCATGA 1 cut(s) 2652
BspLI GGNNCC 2 cut(s) 76, 1673
BspMAI CTGCAG 2 cut(s) 1454, 1687
BspT104I TTCGAA 1 cut(s) 2974
BspTNI GGTCTC 1 cut(s) 3197
BsrDI GCAATG 2 cut(s) 2892, 3363
BsrGI TGTACA 5 cut(s) 896, 1950, 2163, 2433, 3168
BsrI ACTGG 3 cut(s) 245, 284, 741
BssECI CCNNGG 2 cut(s) 1059, 1675
BssMI GATC 8 cut(s) 859, 1329, 1587, 2323, 2445, 2572, 2857, 2962
BssNAI GTATAC 3 cut(s) 1323, 1856, 2787
BssT1I CCWWGG 2 cut(s) 1059, 1675
Bst1107I GTATAC 3 cut(s) 1323, 1856, 2787
Bst2UI CCWGG 3 cut(s) 297, 379, 1397
Bst4CI ACNGT 6 cut(s) 935, 1220, 1497, 1945, 2060, 2712
Bst6I CTCTTC 3 cut(s) 2223, 3225, 3261
BstAUI TGTACA 5 cut(s) 896, 1950, 2163, 2433, 3168
BstBAI YACGTR 1 cut(s) 2055
BstBI TTCGAA 1 cut(s) 2974
BstC8I GCNNGC 6 cut(s) 660, 1244, 1507, 2422, 2922, 2969
BstEII GGTNACC 1 cut(s) 1000
BstENI CCTNNNNNAGG 1 cut(s) 3047
BstF5I GGATG 9 cut(s) 522, 1342, 1405, 1470, 2290, 2304, 2634, 2735, 3322
BstFNI CGCG 1 cut(s) 2733
BstHHI GCGC 2 cut(s) 2696, 2735
BstKTI GATC 8 cut(s) 862, 1332, 1590, 2326, 2448, 2575, 2860, 2965
BstMAI GTCTC 3 cut(s) 530, 1964, 3197
BstMBI GATC 8 cut(s) 859, 1329, 1587, 2323, 2445, 2572, 2857, 2962
BstMWI GCNNNNNNNGC 8 cut(s) 608, 1511, 1563, 1787, 2732, 2903, 2977, 3044
BstNI CCWGG 3 cut(s) 297, 379, 1397
BstNSI RCATGY 8 cut(s) 910, 1475, 1638, 1977, 2424, 2481, 2761, 2971
BstPI GGTNACC 1 cut(s) 1000
BstSCI CCNGG 3 cut(s) 295, 377, 1395
BstSFI CTRYAG 5 cut(s) 189, 531, 1450, 1683, 1935
BstSNI TACGTA 1 cut(s) 2055
BstUI CGCG 1 cut(s) 2733
BstV2I GAAGAC 1 cut(s) 2738
BstX2I RGATCY 1 cut(s) 1587
BstYI RGATCY 1 cut(s) 1587
BstZ17I GTATAC 3 cut(s) 1323, 1856, 2787
Bsu15I ATCGAT 1 cut(s) 1328
Bsu36I CCTNAGG 3 cut(s) 148, 1783, 2205
BsuI GTATCC 4 cut(s) 439, 2251, 2282, 2387
BsuTUI ATCGAT 1 cut(s) 1328
BtgZI GCGATG 1 cut(s) 2963
BtsCI GGATG 9 cut(s) 522, 1342, 1405, 1470, 2290, 2304, 2634, 2735, 3322
BtsI GCAGTG 3 cut(s) 1194, 1617, 3332
BtsIMutI CAGTG 6 cut(s) 206, 252, 1194, 1437, 1617, 3332
Cac8I GCNNGC 6 cut(s) 660, 1244, 1507, 2422, 2922, 2969
CciI TCATGA 1 cut(s) 2652
CfoI GCGC 2 cut(s) 2696, 2735
Cfr13I GGNCC 2 cut(s) 616, 1393
ClaI ATCGAT 1 cut(s) 1328
DpnI GATC 8 cut(s) 861, 1331, 1589, 2325, 2447, 2574, 2859, 2964
DpnII GATC 8 cut(s) 859, 1329, 1587, 2323, 2445, 2572, 2857, 2962
DraI TTTAAA 1 cut(s) 1230
DraIII CACNNNGTG 1 cut(s) 1661
EaeI YGGCCR 5 cut(s) 1053, 1612, 2344, 2527, 2763
Eam1104I CTCTTC 3 cut(s) 2223, 3225, 3261
EarI CTCTTC 3 cut(s) 2223, 3225, 3261
Ecl136II GAGCTC 1 cut(s) 128
Eco105I TACGTA 1 cut(s) 2055
Eco130I CCWWGG 2 cut(s) 1059, 1675
Eco147I AGGCCT 1 cut(s) 3129
Eco24I GRGCYC 2 cut(s) 130, 1134
Eco31I GGTCTC 1 cut(s) 3197
Eco32I GATATC 2 cut(s) 1417, 3303
Eco47I GGWCC 2 cut(s) 616, 1393
Eco53kI GAGCTC 1 cut(s) 128
Eco57I CTGAAG 2 cut(s) 100, 459
Eco81I CCTNAGG 3 cut(s) 148, 1783, 2205
Eco91I GGTNACC 1 cut(s) 1000
EcoICRI GAGCTC 1 cut(s) 128
EcoNI CCTNNNNNAGG 1 cut(s) 3047
EcoO65I GGTNACC 1 cut(s) 1000
EcoRII CCWGG 3 cut(s) 295, 377, 1395
EcoRV GATATC 2 cut(s) 1417, 3303
EcoT14I CCWWGG 2 cut(s) 1059, 1675
EcoT22I ATGCAT 1 cut(s) 3021
EcoT38I GRGCYC 2 cut(s) 130, 1134
ErhI CCWWGG 2 cut(s) 1059, 1675
FalI AAGNNNNNCTT 6 cut(s) 714, 746, 1258, 1290, 3027, 3059
FaqI GGGAC 2 cut(s) 1206, 2341
FauNDI CATATG 3 cut(s) 864, 1704, 2635
FbaI TGATCA 2 cut(s) 859, 2445
FblI GTMKAC 3 cut(s) 1322, 1855, 2786
FokI GGATG 9 cut(s) 529, 1349, 1412, 1477, 2297, 2311, 2641, 2722, 3329
FriOI GRGCYC 2 cut(s) 130, 1134
FspBI CTAG 6 cut(s) 354, 360, 599, 1833, 2174, 2642
GlaI GCGC 2 cut(s) 2695, 2734
GsaI CCCAGC 3 cut(s) 1461, 1671, 2076
HhaI GCGC 2 cut(s) 2696, 2735
Hin6I GCGC 2 cut(s) 2694, 2733
HinP1I GCGC 2 cut(s) 2694, 2733
HindIII AAGCTT 2 cut(s) 728, 1148
HphI GGTGA 6 cut(s) 1086, 1787, 1820, 2139, 3155, 3275
Hpy166II GTNNAC 8 cut(s) 502, 1323, 1493, 1856, 2166, 2434, 2787, 3216
Hpy188III TCNNGA 6 cut(s) 1412, 2012, 2191, 2653, 2960, 3306
Hpy8I GTNNAC 8 cut(s) 502, 1323, 1493, 1856, 2166, 2434, 2787, 3216
Hpy99I CGWCG 2 cut(s) 577, 1933
HpyCH4III ACNGT 6 cut(s) 935, 1220, 1497, 1945, 2060, 2712
HpyCH4IV ACGT 2 cut(s) 2054, 3212
HpyF10VI GCNNNNNNNGC 8 cut(s) 608, 1511, 1563, 1787, 2732, 2903, 2977, 3044
HpySE526I ACGT 2 cut(s) 2054, 3212
HspAI GCGC 2 cut(s) 2694, 2733
Ksp22I TGATCA 2 cut(s) 859, 2445
Kzo9I GATC 8 cut(s) 859, 1329, 1587, 2323, 2445, 2572, 2857, 2962
LmnI GCTCC 2 cut(s) 1787, 2840
LweI GCATC 5 cut(s) 638, 1768, 2744, 2940, 3006
MaeI CTAG 6 cut(s) 354, 360, 599, 1833, 2174, 2642
MaeII ACGT 2 cut(s) 2054, 3212
MalI GATC 8 cut(s) 861, 1331, 1589, 2325, 2447, 2574, 2859, 2964
MboI GATC 8 cut(s) 859, 1329, 1587, 2323, 2445, 2572, 2857, 2962
MfeI CAATTG 3 cut(s) 404, 811, 3344
MflI RGATCY 1 cut(s) 1587
MhlI GDGCHC 3 cut(s) 130, 1134, 1437
MlsI TGGCCA 4 cut(s) 1055, 1614, 2529, 2765
MluNI TGGCCA 4 cut(s) 1055, 1614, 2529, 2765
MlyI GAGTC 5 cut(s) 212, 365, 410, 1161, 2516
MmeI TCCRAC 1 cut(s) 3014
Mox20I TGGCCA 4 cut(s) 1055, 1614, 2529, 2765
Mph1103I ATGCAT 1 cut(s) 3021
MroXI GAANNNNTTC 1 cut(s) 272
MscI TGGCCA 4 cut(s) 1055, 1614, 2529, 2765
MslI CAYNNNNRTG 2 cut(s) 634, 3084
Msp20I TGGCCA 4 cut(s) 1055, 1614, 2529, 2765
MspA1I CMGCKG 1 cut(s) 3166
MspR9I CCNGG 3 cut(s) 297, 379, 1397
MunI CAATTG 3 cut(s) 404, 811, 3344
Mva1269I GAATGC 3 cut(s) 1290, 1923, 2982
MvaI CCWGG 3 cut(s) 297, 379, 1397
MvnI CGCG 1 cut(s) 2733
MwoI GCNNNNNNNGC 8 cut(s) 608, 1511, 1563, 1787, 2732, 2903, 2977, 3044
NdeI CATATG 3 cut(s) 864, 1704, 2635
NdeII GATC 8 cut(s) 859, 1329, 1587, 2323, 2445, 2572, 2857, 2962
NlaIV GGNNCC 2 cut(s) 76, 1673
NmuCI GTSAC 5 cut(s) 204, 2145, 2480, 2878, 2888
NsiI ATGCAT 1 cut(s) 3021
NspI RCATGY 8 cut(s) 910, 1475, 1638, 1977, 2424, 2481, 2761, 2971
NspV TTCGAA 1 cut(s) 2974
PaeI GCATGC 2 cut(s) 2424, 2971
PagI TCATGA 1 cut(s) 2652
PceI AGGCCT 1 cut(s) 3129
PciI ACATGT 4 cut(s) 1471, 1634, 2477, 2757
PcsI WCGNNNNNNNCGW 1 cut(s) 2971
PctI GAATGC 3 cut(s) 1290, 1923, 2982
PdmI GAANNNNTTC 1 cut(s) 272
PfeI GAWTC 6 cut(s) 595, 1367, 1408, 1446, 1603, 2656
PflMI CCANNNNNTGG 1 cut(s) 251
PfoI TCCNGGA 1 cut(s) 1395
PleI GAGTC 5 cut(s) 211, 365, 410, 1161, 2515
PpsI GAGTC 5 cut(s) 211, 365, 410, 1161, 2515
Ppu21I YACGTR 1 cut(s) 2055
PscI ACATGT 4 cut(s) 1471, 1634, 2477, 2757
PshBI ATTAAT 2 cut(s) 1020, 2808
PsiI TTATAA 2 cut(s) 798, 1113
Psp124BI GAGCTC 1 cut(s) 130
Psp6I CCWGG 3 cut(s) 295, 377, 1395
PspEI GGTNACC 1 cut(s) 1000
PspFI CCCAGC 3 cut(s) 1457, 1667, 2072
PspGI CCWGG 3 cut(s) 295, 377, 1395
PspN4I GGNNCC 2 cut(s) 76, 1673
PspPI GGNCC 2 cut(s) 616, 1393
PstI CTGCAG 2 cut(s) 1454, 1687
PsuI RGATCY 1 cut(s) 1587
PvuII CAGCTG 1 cut(s) 3166
RseI CAYNNNNRTG 2 cut(s) 634, 3084
SacI GAGCTC 1 cut(s) 130
Sau3AI GATC 8 cut(s) 859, 1329, 1587, 2323, 2445, 2572, 2857, 2962
Sau96I GGNCC 2 cut(s) 616, 1393
ScaI AGTACT 1 cut(s) 1273
SchI GAGTC 5 cut(s) 212, 365, 410, 1161, 2516
ScrFI CCNGG 3 cut(s) 297, 379, 1397
SduI GDGCHC 3 cut(s) 130, 1134, 1437
SfaNI GCATC 5 cut(s) 638, 1768, 2744, 2940, 3006
SfcI CTRYAG 5 cut(s) 189, 531, 1450, 1683, 1935
SfuI TTCGAA 1 cut(s) 2974
SinI GGWCC 2 cut(s) 616, 1393
SmiMI CAYNNNNRTG 2 cut(s) 634, 3084
SmlI CTYRAG 2 cut(s) 505, 2293
SmoI CTYRAG 2 cut(s) 505, 2293
SnaBI TACGTA 1 cut(s) 2055
SphI GCATGC 2 cut(s) 2424, 2971
SseBI AGGCCT 1 cut(s) 3129
SsiI CCGC 1 cut(s) 2731
SspMI CTAG 6 cut(s) 354, 360, 599, 1833, 2174, 2642
SstI GAGCTC 1 cut(s) 130
StuI AGGCCT 1 cut(s) 3129
StyD4I CCNGG 3 cut(s) 295, 377, 1395
StyI CCWWGG 2 cut(s) 1059, 1675
TaaI ACNGT 6 cut(s) 935, 1220, 1497, 1945, 2060, 2712
TaiI ACGT 2 cut(s) 2057, 3215
TaqI TCGA 4 cut(s) 1328, 1575, 2974, 3096
TaqII GACCGA 1 cut(s) 210
TatI WGTACW 7 cut(s) 896, 1271, 1950, 2163, 2250, 2433, 3168
TfiI GAWTC 6 cut(s) 595, 1367, 1408, 1446, 1603, 2656
TscAI CASTG 6 cut(s) 213, 252, 1194, 1437, 1624, 3339
TseFI GTSAC 5 cut(s) 204, 2145, 2480, 2878, 2888
Tsp45I GTSAC 5 cut(s) 204, 2145, 2480, 2878, 2888
TspGWI ACGGA 1 cut(s) 1917
TspRI CASTG 6 cut(s) 213, 252, 1194, 1437, 1624, 3339
Van91I CCANNNNNTGG 1 cut(s) 251
VpaK11BI GGWCC 2 cut(s) 616, 1393
VspI ATTAAT 2 cut(s) 1020, 2808
XagI CCTNNNNNAGG 1 cut(s) 3047
XapI RAATTY 7 cut(s) 33, 269, 1901, 1987, 2139, 2718, 3310
XceI RCATGY 8 cut(s) 910, 1475, 1638, 1977, 2424, 2481, 2761, 2971
XcmI CCANNNNNNNNNTGG 1 cut(s) 385
XmiI GTMKAC 3 cut(s) 1322, 1855, 2786
XmnI GAANNNNTTC 1 cut(s) 272
XspI CTAG 6 cut(s) 354, 360, 599, 1833, 2174, 2642
ZrmI AGTACT 1 cut(s) 1273
Zsp2I ATGCAT 1 cut(s) 3021
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.