MD03G1043300.v1.1

PRA1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0016200
Physical Location & Seq
Reverse (-)
3395296 .. 3395580
285 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1043300.v1.1.491

Sequence Viewer

Length: 285 bp
ATGTCGCCGTCAAAGCCACAGTCACACTCCGTCACAGCAACAACCTATACCAGCATACCCATTTCCGTCTCGGGCTCCGACGCCATCACCCGATCCATCAAAAATCTCAACGACGCCGTTTCCCGCCGCCGCCCTTCGTCCGAATTCGCCTCCTCGATCTCCCGACCCGACTCCTTATCCTCCGCCCTGACCCGTATCCAGACCAACGCCAACCACTTCCGGGTCAATTACTTGTTATTGATCGGATCATGCGGGGCGCTATCCCTCATCGGGAGCCCCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006810 GO:0008150 GO:0012505 GO:0016192 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

95

Amino Acids

10.01

Weight (kDa)

10.84

Isoelectric Point (pI)

64.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 39 - 93 1.8e-12 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016200)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56230
fragaria_vesca FvH4_3g41570
malus_domestica MD03G1043200.v1.1 MD03G1043300.v1.1 MD11G1046400.v1.1
prunus_persica Prupe.6G035700_v2.0.a1
pyrus_communis pycom03g03350 pycom11g03780
rosa_chinensis RchiOBHm_Chr5g0074561
rosa_laevigata RLG00000036487
rosa_roxburghii Rroxscaffold_1G00007000
rosa_rugosa Rorug05G0432700
rosa_samantha Rh5BG510700 Rh5CG534500
rosa_wichuraiana Rw5G045410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 124, 127, 130, 183, 252
AclWI GGATC 2 cut(s) 87, 253
AcsI RAATTY 1 cut(s) 143
AcyI GRCGYC 2 cut(s) 81, 114
AfiI CCNNNNNNNGG 2 cut(s) 220, 270
Alw26I GTCTC 1 cut(s) 73
AlwI GGATC 2 cut(s) 87, 253
Ama87I CYCGRG 1 cut(s) 70
ApoI RAATTY 1 cut(s) 143
ArsI GACNNNNNNTTYG 1 cut(s) 37
AspLEI GCGC 1 cut(s) 259
AsuC2I CCSGG 1 cut(s) 221
AsuHPI GGTGA 1 cut(s) 79
AvaI CYCGRG 1 cut(s) 70
BanII GRGCYC 2 cut(s) 77, 278
BccI CCATC 2 cut(s) 92, 104
BceAI ACGGC 1 cut(s) 101
BciVI GTATCC 1 cut(s) 206
BcnI CCSGG 1 cut(s) 221
BcoDI GTCTC 1 cut(s) 73
BfoI RGCGCY 1 cut(s) 260
BfuI GTATCC 1 cut(s) 206
BisI GCNGC 2 cut(s) 127, 130
BlsI GCNGC 2 cut(s) 128, 131
Bme1390I CCNGG 1 cut(s) 221
BmeT110I CYCGRG 1 cut(s) 70
BmiI GGNNCC 2 cut(s) 76, 275
BmrFI CCNGG 1 cut(s) 221
BpuMI CCSGG 1 cut(s) 221
BsaHI GRCGYC 2 cut(s) 81, 114
Bsc4I CCNNNNNNNGG 2 cut(s) 220, 270
BseLI CCNNNNNNNGG 2 cut(s) 220, 270
BseRI GAGGAG 1 cut(s) 142
BsiHKCI CYCGRG 1 cut(s) 70
BsiSI CCGG 1 cut(s) 220
BslI CCNNNNNNNGG 2 cut(s) 220, 270
BsmAI GTCTC 1 cut(s) 73
BsmBI CGTCTC 1 cut(s) 73
BsoBI CYCGRG 1 cut(s) 70
Bsp1286I GDGCHC 2 cut(s) 77, 278
Bsp143I GATC 4 cut(s) 92, 156, 240, 245
BspACI CCGC 5 cut(s) 124, 127, 130, 183, 252
BspLI GGNNCC 2 cut(s) 76, 275
BspPI GGATC 2 cut(s) 87, 253
BssMI GATC 4 cut(s) 92, 156, 240, 245
BssNI GRCGYC 2 cut(s) 81, 114
Bst4CI ACNGT 1 cut(s) 21
BstACI GRCGYC 2 cut(s) 81, 114
BstH2I RGCGCY 1 cut(s) 260
BstHHI GCGC 1 cut(s) 259
BstKTI GATC 4 cut(s) 95, 159, 243, 248
BstMAI GTCTC 1 cut(s) 73
BstMBI GATC 4 cut(s) 92, 156, 240, 245
BstMWI GCNNNNNNNGC 1 cut(s) 13
BstSCI CCNGG 1 cut(s) 219
BsuI GTATCC 1 cut(s) 206
CfoI GCGC 1 cut(s) 259
CseI GACGC 2 cut(s) 89, 122
CviAII CATG 1 cut(s) 249
CviJI RGCY 3 cut(s) 16, 75, 276
CviKI_1 RGCY 3 cut(s) 16, 75, 276
DpnI GATC 4 cut(s) 94, 158, 242, 247
DpnII GATC 4 cut(s) 92, 156, 240, 245
EciI GGCGGA 1 cut(s) 172
Eco24I GRGCYC 2 cut(s) 77, 278
Eco88I CYCGRG 1 cut(s) 70
EcoRI GAATTC 1 cut(s) 143
EcoT38I GRGCYC 2 cut(s) 77, 278
Esp3I CGTCTC 1 cut(s) 73
FaeI CATG 1 cut(s) 252
FaiI YATR 3 cut(s) 48, 56, 250
FatI CATG 1 cut(s) 248
FauI CCCGC 2 cut(s) 131, 245
Fnu4HI GCNGC 2 cut(s) 127, 130
FriOI GRGCYC 2 cut(s) 77, 278
Fsp4HI GCNGC 2 cut(s) 127, 130
GlaI GCGC 1 cut(s) 258
GluI GCNGC 2 cut(s) 127, 130
HaeII RGCGCY 1 cut(s) 260
HapII CCGG 1 cut(s) 220
HgaI GACGC 2 cut(s) 89, 122
HhaI GCGC 1 cut(s) 259
Hin1I GRCGYC 2 cut(s) 81, 114
Hin1II CATG 1 cut(s) 252
Hin6I GCGC 1 cut(s) 257
HinP1I GCGC 1 cut(s) 257
HinfI GANTC 1 cut(s) 170
HpaII CCGG 1 cut(s) 220
HphI GGTGA 1 cut(s) 79
Hpy188I TCNGA 3 cut(s) 79, 142, 245
Hpy188III TCNNGA 3 cut(s) 162, 199, 271
Hpy99I CGWCG 2 cut(s) 83, 116
HpyAV CCTTC 1 cut(s) 144
HpyCH4III ACNGT 1 cut(s) 21
HpyF10VI GCNNNNNNNGC 1 cut(s) 13
Hsp92I GRCGYC 2 cut(s) 81, 114
Hsp92II CATG 1 cut(s) 252
HspAI GCGC 1 cut(s) 257
Kzo9I GATC 4 cut(s) 92, 156, 240, 245
LmnI GCTCC 2 cut(s) 80, 273
LpnPI CCDG 4 cut(s) 64, 200, 212, 233
MaeIII GTNAC 2 cut(s) 21, 31
MalI GATC 4 cut(s) 94, 158, 242, 247
MboI GATC 4 cut(s) 92, 156, 240, 245
MhlI GDGCHC 2 cut(s) 77, 278
MluCI AATT 2 cut(s) 143, 226
MlyI GAGTC 1 cut(s) 164
MmeI TCCRAC 1 cut(s) 102
MnlI CCTC 4 cut(s) 160, 163, 190, 275
MspI CCGG 1 cut(s) 220
MspR9I CCNGG 1 cut(s) 221
MwoI GCNNNNNNNGC 1 cut(s) 13
NciI CCSGG 1 cut(s) 221
NdeII GATC 4 cut(s) 92, 156, 240, 245
NlaIII CATG 1 cut(s) 252
NlaIV GGNNCC 2 cut(s) 76, 275
NmuCI GTSAC 2 cut(s) 21, 31
PkrI GCNGC 2 cut(s) 128, 131
PleI GAGTC 1 cut(s) 164
PpsI GAGTC 1 cut(s) 164
PspN4I GGNNCC 2 cut(s) 76, 275
SatI GCNGC 2 cut(s) 127, 130
Sau3AI GATC 4 cut(s) 92, 156, 240, 245
SchI GAGTC 1 cut(s) 164
ScrFI CCNGG 1 cut(s) 221
SduI GDGCHC 2 cut(s) 77, 278
SetI ASST 1 cut(s) 47
Sse9I AATT 2 cut(s) 143, 226
SsiI CCGC 5 cut(s) 124, 127, 130, 183, 252
StyD4I CCNGG 1 cut(s) 219
TaaI ACNGT 1 cut(s) 21
TaqI TCGA 1 cut(s) 155
TasI AATT 2 cut(s) 143, 226
TauI GCSGC 2 cut(s) 129, 132
TseFI GTSAC 2 cut(s) 21, 31
Tsp45I GTSAC 2 cut(s) 21, 31
TspGWI ACGGA 2 cut(s) 19, 55
XapI RAATTY 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.