pycom11g03780

PRA1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0016200
Physical Location & Seq
Reverse (-)
2923798 .. 2924550
753 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g03780.1

Sequence Viewer

Length: 753 bp
ATGGAAACTCCACTAACGAACCCAAATCTTCACCTTTCATGCTTCCCTTCCCTGTCTGTCCCTCACAGCCCCACTCACCAACCAATCAAACTCAGAGACTGTCACAGCCTCACAGACTTCACAGAGTTGAAGGGTTTGAAGGGTAGGTTAACGAACCAAACATTCAAACCCTCACTACAAAAAATGTCGCCGTCGCAGTCCGGCACAGCAACAACCTATACCAGCATACCCATTTCGGTCTCGGGCTCCGACGCCATCACCCGATCCATTAAAAACCTCAACGACGCCGTTTCCCGCCGCCGTCCATGGTCCGAATTCGCCTCCTCGATCTCCCGACCCGACTCCTTATCCTCCGCCCTGACCCGGATCCAGACCAACGCCAACCACTTCCGGGTCAACTACTTGTTATTGATCGGATCCTGCGGGGTGCTATCCCTCATCGGGAGCCCGGGATGGCTTCTGGTGACGGCGGCCGTCGTGGGGTTGTGGCTGATGATCTATGTTTTTAGGGAGGACCCACTTGAGGTGTGGGGCCACCACGTCAGCAATTGGGCCGTGATGGCGGGCCTGGTAATTGTTTCTGTTTTGGTTGCTTGGGCCAGCGGTGGGCTTGGGAGTGTTGCATTGGGCCTTGGAGTTGGGCTTCTGTTGTGTGCGGTCCACGGGCTGCTTACGAACACAGAAGGCTTGTTTCTCAACGAGACCGAAGCTGCTTCTCAGGGATTGATTGGGCCCACATCTTCATCCGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006810 GO:0008150 GO:0012505 GO:0016192 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

251

Amino Acids

26.67

Weight (kDa)

8.42

Isoelectric Point (pI)

41.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 96 - 234 4.1e-35 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016200)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56230
fragaria_vesca FvH4_3g41570
malus_domestica MD03G1043200.v1.1 MD03G1043300.v1.1 MD11G1046400.v1.1
prunus_persica Prupe.6G035700_v2.0.a1
pyrus_communis pycom03g03350 pycom11g03780
rosa_chinensis RchiOBHm_Chr5g0074561
rosa_laevigata RLG00000036487
rosa_roxburghii Rroxscaffold_1G00007000
rosa_rugosa Rorug05G0432700
rosa_samantha Rh5BG510700 Rh5CG534500
rosa_wichuraiana Rw5G045410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 8 cut(s) 295, 298, 354, 423, 470, 563, 603, 656
AclWI GGATC 5 cut(s) 258, 361, 374, 411, 424
AcoI YGGCCR 1 cut(s) 471
AcsI RAATTY 1 cut(s) 314
AcyI GRCGYC 2 cut(s) 252, 285
AfiI CCNNNNNNNGG 6 cut(s) 363, 391, 441, 480, 523, 606
AgsI TTSAA 3 cut(s) 130, 139, 166
AjiI CACGTC 1 cut(s) 541
AjnI CCWGG 1 cut(s) 567
AluBI AGCT 1 cut(s) 710
AluI AGCT 1 cut(s) 710
Alw26I GTCTC 3 cut(s) 90, 244, 695
AlwI GGATC 5 cut(s) 258, 361, 374, 411, 424
AlwNI CAGNNNCTG 1 cut(s) 99
Ama87I CYCGRG 2 cut(s) 241, 448
AoxI GGCC 7 cut(s) 471, 532, 552, 565, 597, 628, 731
ApaI GGGCCC 1 cut(s) 735
ApeKI GCWGC 2 cut(s) 667, 710
ApoI RAATTY 1 cut(s) 314
AsuC2I CCSGG 4 cut(s) 364, 392, 449, 450
AsuHPI GGTGA 4 cut(s) 23, 68, 250, 475
AvaI CYCGRG 2 cut(s) 241, 448
AvaII GGWCC 3 cut(s) 309, 514, 658
BaeGI GKGCMC 1 cut(s) 735
BamHI GGATCC 2 cut(s) 366, 416
BanII GRGCYC 3 cut(s) 248, 449, 735
BbvI GCAGC 2 cut(s) 654, 697
BccI CCATC 3 cut(s) 263, 447, 553
BceAI ACGGC 6 cut(s) 175, 272, 285, 458, 483, 539
BciT130I CCWGG 1 cut(s) 569
BcnI CCSGG 4 cut(s) 364, 392, 449, 450
BcoDI GTCTC 3 cut(s) 90, 244, 695
BglI GCCNNNNNGGC 1 cut(s) 560
BisI GCNGC 4 cut(s) 298, 471, 668, 711
BlsI GCNGC 4 cut(s) 299, 472, 669, 712
Bme1390I CCNGG 5 cut(s) 364, 392, 449, 450, 569
Bme18I GGWCC 3 cut(s) 309, 514, 658
BmeT110I CYCGRG 2 cut(s) 241, 448
BmgBI CACGTC 1 cut(s) 541
BmiI GGNNCC 7 cut(s) 247, 368, 418, 446, 516, 533, 733
BmrFI CCNGG 5 cut(s) 364, 392, 449, 450, 569
BpuEI CTTGAG 1 cut(s) 542
BpuMI CCSGG 4 cut(s) 364, 392, 449, 450
BsaHI GRCGYC 2 cut(s) 252, 285
BsaI GGTCTC 2 cut(s) 244, 695
BsaJI CCNNGG 4 cut(s) 305, 448, 631, 661
Bsc4I CCNNNNNNNGG 6 cut(s) 363, 391, 441, 480, 523, 606
BseBI CCWGG 1 cut(s) 569
BseDI CCNNGG 4 cut(s) 305, 448, 631, 661
BseGI GGATG 2 cut(s) 458, 743
BseLI CCNNNNNNNGG 6 cut(s) 363, 391, 441, 480, 523, 606
BseMII CTCAG 2 cut(s) 106, 731
BseRI GAGGAG 1 cut(s) 313
BseSI GKGCMC 1 cut(s) 735
BseX3I CGGCCG 1 cut(s) 471
BseXI GCAGC 2 cut(s) 654, 697
Bsh1285I CGRYCG 1 cut(s) 474
BshFI GGCC 7 cut(s) 473, 534, 554, 567, 599, 630, 733
BsiEI CGRYCG 1 cut(s) 474
BsiHKCI CYCGRG 2 cut(s) 241, 448
BsiSI CCGG 4 cut(s) 201, 364, 391, 449
BslFI GGGAC 1 cut(s) 44
BslI CCNNNNNNNGG 6 cut(s) 363, 391, 441, 480, 523, 606
BsmAI GTCTC 3 cut(s) 90, 244, 695
BsmFI GGGAC 1 cut(s) 44
BsnI GGCC 7 cut(s) 473, 534, 554, 567, 599, 630, 733
Bso31I GGTCTC 2 cut(s) 244, 695
BsoBI CYCGRG 2 cut(s) 241, 448
Bsp120I GGGCCC 1 cut(s) 731
Bsp1286I GDGCHC 3 cut(s) 248, 449, 735
Bsp143I GATC 6 cut(s) 263, 327, 366, 411, 416, 495
Bsp19I CCATGG 1 cut(s) 305
BspACI CCGC 8 cut(s) 295, 298, 354, 423, 470, 563, 603, 656
BspANI GGCC 7 cut(s) 473, 534, 554, 567, 599, 630, 733
BspCNI CTCAG 2 cut(s) 105, 730
BspLI GGNNCC 7 cut(s) 247, 368, 418, 446, 516, 533, 733
BspPI GGATC 5 cut(s) 258, 361, 374, 411, 424
BspTNI GGTCTC 2 cut(s) 244, 695
BssECI CCNNGG 4 cut(s) 305, 448, 631, 661
BssMI GATC 6 cut(s) 263, 327, 366, 411, 416, 495
BssNI GRCGYC 2 cut(s) 252, 285
BssT1I CCWWGG 2 cut(s) 305, 631
Bst2UI CCWGG 1 cut(s) 569
Bst4CI ACNGT 1 cut(s) 101
BstACI GRCGYC 2 cut(s) 252, 285
BstC8I GCNNGC 2 cut(s) 565, 601
BstDEI CTNAG 2 cut(s) 92, 717
BstDSI CCRYGG 2 cut(s) 305, 661
BstF5I GGATG 2 cut(s) 458, 743
BstKTI GATC 6 cut(s) 266, 330, 369, 414, 419, 498
BstMAI GTCTC 3 cut(s) 90, 244, 695
BstMBI GATC 6 cut(s) 263, 327, 366, 411, 416, 495
BstMCI CGRYCG 1 cut(s) 474
BstMWI GCNNNNNNNGC 1 cut(s) 560
BstNI CCWGG 1 cut(s) 569
BstSCI CCNGG 5 cut(s) 362, 390, 447, 448, 567
BstSLI GKGCMC 1 cut(s) 735
BstV1I GCAGC 2 cut(s) 654, 697
BstX2I RGATCY 2 cut(s) 366, 416
BstYI RGATCY 2 cut(s) 366, 416
BstZI CGGCCG 1 cut(s) 471
BsuRI GGCC 7 cut(s) 473, 534, 554, 567, 599, 630, 733
BtgI CCRYGG 2 cut(s) 305, 661
BtrI CACGTC 1 cut(s) 541
BtsCI GGATG 2 cut(s) 458, 743
Cac8I GCNNGC 2 cut(s) 565, 601
CaiI CAGNNNCTG 1 cut(s) 99
Cfr9I CCCGGG 1 cut(s) 448
CseI GACGC 2 cut(s) 260, 293
CviAII CATG 2 cut(s) 39, 306
DdeI CTNAG 2 cut(s) 92, 717
DpnI GATC 6 cut(s) 265, 329, 368, 413, 418, 497
DpnII GATC 6 cut(s) 263, 327, 366, 411, 416, 495
EaeI YGGCCR 1 cut(s) 471
EagI CGGCCG 1 cut(s) 471
EciI GGCGGA 1 cut(s) 343
EclXI CGGCCG 1 cut(s) 471
Eco130I CCWWGG 2 cut(s) 305, 631
Eco24I GRGCYC 3 cut(s) 248, 449, 735
Eco31I GGTCTC 2 cut(s) 244, 695
Eco47I GGWCC 3 cut(s) 309, 514, 658
Eco52I CGGCCG 1 cut(s) 471
Eco88I CYCGRG 2 cut(s) 241, 448
EcoO109I RGGNCCY 1 cut(s) 514
EcoRI GAATTC 1 cut(s) 314
EcoRII CCWGG 1 cut(s) 567
EcoT14I CCWWGG 2 cut(s) 305, 631
EcoT38I GRGCYC 3 cut(s) 248, 449, 735
ErhI CCWWGG 2 cut(s) 305, 631
FaeI CATG 2 cut(s) 42, 309
FaiI YATR 5 cut(s) 40, 219, 227, 307, 501
FaqI GGGAC 1 cut(s) 44
FatI CATG 2 cut(s) 38, 305
FauI CCCGC 3 cut(s) 302, 416, 556
Fnu4HI GCNGC 4 cut(s) 298, 471, 668, 711
FokI GGATG 2 cut(s) 465, 730
FriOI GRGCYC 3 cut(s) 248, 449, 735
Fsp4HI GCNGC 4 cut(s) 298, 471, 668, 711
GluI GCNGC 4 cut(s) 298, 471, 668, 711
HaeIII GGCC 7 cut(s) 473, 534, 554, 567, 599, 630, 733
HapII CCGG 4 cut(s) 201, 364, 391, 449
HgaI GACGC 2 cut(s) 260, 293
Hin1I GRCGYC 2 cut(s) 252, 285
Hin1II CATG 2 cut(s) 42, 309
HincII GTYRAC 2 cut(s) 150, 397
HindII GTYRAC 2 cut(s) 150, 397
HinfI GANTC 1 cut(s) 341
HpaI GTTAAC 1 cut(s) 150
HpaII CCGG 4 cut(s) 201, 364, 391, 449
HphI GGTGA 4 cut(s) 23, 68, 250, 475
Hpy166II GTNNAC 3 cut(s) 150, 397, 661
Hpy188I TCNGA 4 cut(s) 95, 250, 313, 416
Hpy188III TCNNGA 3 cut(s) 333, 370, 442
Hpy8I GTNNAC 3 cut(s) 150, 397, 661
Hpy99I CGWCG 4 cut(s) 196, 254, 287, 479
HpyAV CCTTC 4 cut(s) 57, 124, 133, 677
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4IV ACGT 1 cut(s) 540
HpyCH4V TGCA 1 cut(s) 623
HpyF10VI GCNNNNNNNGC 1 cut(s) 560
HpyF3I CTNAG 2 cut(s) 92, 717
HpySE526I ACGT 1 cut(s) 540
Hsp92I GRCGYC 2 cut(s) 252, 285
Hsp92II CATG 2 cut(s) 42, 309
KspAI GTTAAC 1 cut(s) 150
Kzo9I GATC 6 cut(s) 263, 327, 366, 411, 416, 495
LmnI GCTCC 2 cut(s) 251, 444
Lsp1109I GCAGC 2 cut(s) 654, 697
MaeII ACGT 1 cut(s) 540
MaeIII GTNAC 2 cut(s) 101, 463
MalI GATC 6 cut(s) 265, 329, 368, 413, 418, 497
MboI GATC 6 cut(s) 263, 327, 366, 411, 416, 495
MboII GAAGA 2 cut(s) 20, 732
MfeI CAATTG 1 cut(s) 547
MflI RGATCY 2 cut(s) 366, 416
MhlI GDGCHC 3 cut(s) 248, 449, 735
MluCI AATT 3 cut(s) 314, 547, 573
MlyI GAGTC 1 cut(s) 335
MmeI TCCRAC 1 cut(s) 273
MseI TTAA 2 cut(s) 149, 270
MspA1I CMGCKG 1 cut(s) 603
MspI CCGG 4 cut(s) 201, 364, 391, 449
MspR9I CCNGG 5 cut(s) 364, 392, 449, 450, 569
MunI CAATTG 1 cut(s) 547
MvaI CCWGG 1 cut(s) 569
MwoI GCNNNNNNNGC 1 cut(s) 560
NciI CCSGG 4 cut(s) 364, 392, 449, 450
NcoI CCATGG 1 cut(s) 305
NdeII GATC 6 cut(s) 263, 327, 366, 411, 416, 495
NlaIII CATG 2 cut(s) 42, 309
NlaIV GGNNCC 7 cut(s) 247, 368, 418, 446, 516, 533, 733
NmuCI GTSAC 2 cut(s) 101, 463
PkrI GCNGC 4 cut(s) 299, 472, 669, 712
PleI GAGTC 1 cut(s) 335
PpsI GAGTC 1 cut(s) 335
PpuMI RGGWCCY 1 cut(s) 514
Psp5II RGGWCCY 1 cut(s) 514
Psp6I CCWGG 1 cut(s) 567
PspGI CCWGG 1 cut(s) 567
PspN4I GGNNCC 7 cut(s) 247, 368, 418, 446, 516, 533, 733
PspOMI GGGCCC 1 cut(s) 731
PspPPI RGGWCCY 1 cut(s) 514
PstNI CAGNNNCTG 1 cut(s) 99
PsuI RGATCY 2 cut(s) 366, 416
SaqAI TTAA 2 cut(s) 149, 270
SatI GCNGC 4 cut(s) 298, 471, 668, 711
Sau3AI GATC 6 cut(s) 263, 327, 366, 411, 416, 495
SchI GAGTC 1 cut(s) 335
ScrFI CCNGG 5 cut(s) 364, 392, 449, 450, 569
SduI GDGCHC 3 cut(s) 248, 449, 735
SetI ASST 7 cut(s) 36, 149, 218, 279, 528, 543, 712
SinI GGWCC 3 cut(s) 309, 514, 658
SmaI CCCGGG 1 cut(s) 450
SmlI CTYRAG 1 cut(s) 521
SmoI CTYRAG 1 cut(s) 521
Sse9I AATT 3 cut(s) 314, 547, 573
SsiI CCGC 8 cut(s) 295, 298, 354, 423, 470, 563, 603, 656
StyD4I CCNGG 5 cut(s) 362, 390, 447, 448, 567
StyI CCWWGG 2 cut(s) 305, 631
TaaI ACNGT 1 cut(s) 101
TaiI ACGT 1 cut(s) 543
TaqI TCGA 1 cut(s) 326
TaqII GACCGA 2 cut(s) 226, 719
TasI AATT 3 cut(s) 314, 547, 573
TauI GCSGC 2 cut(s) 300, 473
Tru1I TTAA 2 cut(s) 149, 270
Tru9I TTAA 2 cut(s) 149, 270
TseFI GTSAC 2 cut(s) 101, 463
TseI GCWGC 2 cut(s) 667, 710
Tsp45I GTSAC 2 cut(s) 101, 463
TspDTI ATGAA 2 cut(s) 27, 732
TspGWI ACGGA 1 cut(s) 736
TspMI CCCGGG 1 cut(s) 448
VpaK11BI GGWCC 3 cut(s) 309, 514, 658
XapI RAATTY 1 cut(s) 314
XcmI CCANNNNNNNNNTGG 1 cut(s) 525
XmaI CCCGGG 1 cut(s) 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.