Rroxscaffold_1G00007000

PRA1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0016200
Physical Location & Seq
Reverse (-)
8960246 .. 8965533
5288 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00007000.1

Sequence Viewer

Length: 567 bp
ATGGCTAAATCGCCGGAAAAGCTCAAACTGCCCCCGTCGGGTTCCTTGCTTCGATTCGGTGTTTTCCGGCCAAATCATCGAAACACAAGGGCACTAGCGTCAAGAGGAGGAAGAGGCGATCACGTGGTGGCCGGTTGCCCACCGGTTAATGGCGGACGGCGGCGAATCAAAGGGGAGAAGAAATCACCCGAAGAGGAAAGGGGAGAGATCGGGGGAGAGGAGAGAGAAAAGTGGGTTTCCGGATTTGGAAACCTACCACACCCCGGCTGGCTTCTAGCGACGGCCGCCGTCGTCGGGCTGTGGCTGGTGATCTACTTCTTCAGGGAGGACCCACTCGTGGTGTTGGGCCGCCACTTTAGTGATTGGGCCGTGTTTGTGGGCCTAGTGCTGGTTTCTCTTGCGGTTGTGTGGTTGAGCAGTGGGCTTGGGAATGTGGTGTTGGGCCTTGGAATTGGGCTGCTCGTTTGTGGGCTTCATGGGCTGGTTCGGGATACCGAAGGCTTGTTTCTCAACGAGAATGAAGCCGCCTCGGCCGGGTTGATCGGGCCCACCTATACACCCGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006810 GO:0008150 GO:0012505 GO:0016192 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

188

Amino Acids

20.17

Weight (kDa)

9.34

Isoelectric Point (pI)

39.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 85 - 173 7.5e-19 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016200)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56230
fragaria_vesca FvH4_3g41570
malus_domestica MD03G1043200.v1.1 MD03G1043300.v1.1 MD11G1046400.v1.1
prunus_persica Prupe.6G035700_v2.0.a1
pyrus_communis pycom03g03350 pycom11g03780
rosa_chinensis RchiOBHm_Chr5g0074561
rosa_laevigata RLG00000036487
rosa_roxburghii Rroxscaffold_1G00007000
rosa_rugosa Rorug05G0432700
rosa_samantha Rh5BG510700 Rh5CG534500
rosa_wichuraiana Rw5G045410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 239
AciI CCGC 6 cut(s) 153, 160, 285, 349, 401, 525
AcoI YGGCCR 4 cut(s) 68, 129, 282, 531
AcuI CTGAAG 1 cut(s) 304
AcvI CACGTG 1 cut(s) 124
AdeI CACNNNGTG 1 cut(s) 127
AfiI CCNNNNNNNGG 7 cut(s) 38, 149, 263, 294, 337, 388, 534
AgeI ACCGGT 1 cut(s) 142
AjuI GAANNNNNNNTTGG 2 cut(s) 422, 454
AleI CACNNNNGTG 1 cut(s) 357
AluBI AGCT 1 cut(s) 22
AluI AGCT 1 cut(s) 22
Aor13HI TCCGGA 1 cut(s) 239
AoxI GGCC 9 cut(s) 68, 129, 282, 346, 366, 379, 442, 531, 545
ApaI GGGCCC 1 cut(s) 549
ApeKI GCWGC 1 cut(s) 457
AsiGI ACCGGT 1 cut(s) 142
AspS9I GGNCC 7 cut(s) 328, 346, 366, 379, 442, 545, 546
AsuC2I CCSGG 2 cut(s) 264, 535
AsuHPI GGTGA 2 cut(s) 177, 319
AvaII GGWCC 1 cut(s) 328
BaeGI GKGCMC 2 cut(s) 94, 549
BanII GRGCYC 1 cut(s) 549
BauI CACGAG 1 cut(s) 335
BbrPI CACGTG 1 cut(s) 124
BbvI GCAGC 1 cut(s) 444
BceAI ACGGC 4 cut(s) 173, 272, 297, 353
BciVI GTATCC 1 cut(s) 484
BcnI CCSGG 2 cut(s) 264, 535
BfaI CTAG 3 cut(s) 95, 275, 383
BfuI GTATCC 1 cut(s) 484
BglI GCCNNNNNGGC 1 cut(s) 530
BisI GCNGC 5 cut(s) 161, 285, 349, 458, 525
BlsI GCNGC 5 cut(s) 162, 286, 350, 459, 526
Bme1390I CCNGG 2 cut(s) 264, 535
Bme18I GGWCC 1 cut(s) 328
BmgT120I GGNCC 7 cut(s) 328, 346, 366, 379, 442, 545, 546
BmiI GGNNCC 3 cut(s) 43, 330, 547
BmrFI CCNGG 2 cut(s) 264, 535
BpuMI CCSGG 2 cut(s) 264, 535
BsaAI YACGTR 1 cut(s) 124
BsaJI CCNNGG 3 cut(s) 262, 445, 528
BsaWI WCCGGW 2 cut(s) 142, 239
Bsc4I CCNNNNNNNGG 7 cut(s) 38, 149, 263, 294, 337, 388, 534
Bse118I RCCGGY 2 cut(s) 131, 142
BseAI TCCGGA 1 cut(s) 239
BseDI CCNNGG 3 cut(s) 262, 445, 528
BseLI CCNNNNNNNGG 7 cut(s) 38, 149, 263, 294, 337, 388, 534
BseRI GAGGAG 2 cut(s) 120, 233
BseSI GKGCMC 2 cut(s) 94, 549
BseX3I CGGCCG 2 cut(s) 282, 531
BseXI GCAGC 1 cut(s) 444
Bsh1285I CGRYCG 2 cut(s) 285, 534
BshFI GGCC 9 cut(s) 70, 131, 284, 348, 368, 381, 444, 533, 547
BshTI ACCGGT 1 cut(s) 142
BsiEI CGRYCG 2 cut(s) 285, 534
BsiSI CCGG 7 cut(s) 14, 67, 132, 143, 240, 264, 534
BslI CCNNNNNNNGG 7 cut(s) 38, 149, 263, 294, 337, 388, 534
BsnI GGCC 9 cut(s) 70, 131, 284, 348, 368, 381, 444, 533, 547
Bsp120I GGGCCC 1 cut(s) 545
Bsp1286I GDGCHC 2 cut(s) 94, 549
Bsp13I TCCGGA 1 cut(s) 239
Bsp143I GATC 4 cut(s) 118, 207, 309, 540
BspACI CCGC 6 cut(s) 153, 160, 285, 349, 401, 525
BspANI GGCC 9 cut(s) 70, 131, 284, 348, 368, 381, 444, 533, 547
BspEI TCCGGA 1 cut(s) 239
BspLI GGNNCC 3 cut(s) 43, 330, 547
BsrFI RCCGGY 2 cut(s) 131, 142
BssAI RCCGGY 2 cut(s) 131, 142
BssECI CCNNGG 3 cut(s) 262, 445, 528
BssMI GATC 4 cut(s) 118, 207, 309, 540
BssSI CACGAG 1 cut(s) 335
BssT1I CCWWGG 1 cut(s) 445
Bst2BI CACGAG 1 cut(s) 335
Bst6I CTCTTC 2 cut(s) 106, 186
BstBAI YACGTR 1 cut(s) 124
BstC8I GCNNGC 1 cut(s) 269
BstKTI GATC 4 cut(s) 121, 210, 312, 543
BstMBI GATC 4 cut(s) 118, 207, 309, 540
BstMCI CGRYCG 2 cut(s) 285, 534
BstMWI GCNNNNNNNGC 5 cut(s) 19, 28, 284, 478, 530
BstSCI CCNGG 2 cut(s) 262, 533
BstSLI GKGCMC 2 cut(s) 94, 549
BstV1I GCAGC 1 cut(s) 444
BstZI CGGCCG 2 cut(s) 282, 531
BsuI GTATCC 1 cut(s) 484
BsuRI GGCC 9 cut(s) 70, 131, 284, 348, 368, 381, 444, 533, 547
BtsI GCAGTG 1 cut(s) 424
BtsIMutI CAGTG 1 cut(s) 424
Cac8I GCNNGC 1 cut(s) 269
Cfr10I RCCGGY 2 cut(s) 131, 142
Cfr13I GGNCC 7 cut(s) 328, 346, 366, 379, 442, 545, 546
CseI GACGC 1 cut(s) 87
CspAI ACCGGT 1 cut(s) 142
CviAII CATG 1 cut(s) 476
DpnI GATC 4 cut(s) 120, 209, 311, 542
DpnII GATC 4 cut(s) 118, 207, 309, 540
DraIII CACNNNGTG 1 cut(s) 127
EaeI YGGCCR 4 cut(s) 68, 129, 282, 531
EagI CGGCCG 2 cut(s) 282, 531
Eam1104I CTCTTC 2 cut(s) 106, 186
EarI CTCTTC 2 cut(s) 106, 186
EciI GGCGGA 1 cut(s) 168
EclXI CGGCCG 2 cut(s) 282, 531
Eco130I CCWWGG 1 cut(s) 445
Eco24I GRGCYC 1 cut(s) 549
Eco47I GGWCC 1 cut(s) 328
Eco52I CGGCCG 2 cut(s) 282, 531
Eco57I CTGAAG 1 cut(s) 304
Eco72I CACGTG 1 cut(s) 124
EcoO109I RGGNCCY 1 cut(s) 328
EcoT14I CCWWGG 1 cut(s) 445
EcoT38I GRGCYC 1 cut(s) 549
ErhI CCWWGG 1 cut(s) 445
FaeI CATG 1 cut(s) 479
FaiI YATR 2 cut(s) 477, 555
FatI CATG 1 cut(s) 475
Fnu4HI GCNGC 5 cut(s) 161, 285, 349, 458, 525
FriOI GRGCYC 1 cut(s) 549
Fsp4HI GCNGC 5 cut(s) 161, 285, 349, 458, 525
FspBI CTAG 3 cut(s) 95, 275, 383
GluI GCNGC 5 cut(s) 161, 285, 349, 458, 525
HaeIII GGCC 9 cut(s) 70, 131, 284, 348, 368, 381, 444, 533, 547
HapII CCGG 7 cut(s) 14, 67, 132, 143, 240, 264, 534
HgaI GACGC 1 cut(s) 87
Hin1II CATG 1 cut(s) 479
HinfI GANTC 2 cut(s) 54, 165
HpaII CCGG 7 cut(s) 14, 67, 132, 143, 240, 264, 534
HphI GGTGA 2 cut(s) 177, 319
Hpy188I TCNGA 1 cut(s) 566
Hpy188III TCNNGA 3 cut(s) 102, 240, 488
Hpy99I CGWCG 4 cut(s) 40, 283, 293, 296
HpyAV CCTTC 1 cut(s) 491
HpyCH4IV ACGT 1 cut(s) 123
HpyF10VI GCNNNNNNNGC 5 cut(s) 19, 28, 284, 478, 530
HpySE526I ACGT 1 cut(s) 123
Hsp92II CATG 1 cut(s) 479
Kpn2I TCCGGA 1 cut(s) 239
Kzo9I GATC 4 cut(s) 118, 207, 309, 540
Lsp1109I GCAGC 1 cut(s) 444
MaeI CTAG 3 cut(s) 95, 275, 383
MaeII ACGT 1 cut(s) 123
MalI GATC 4 cut(s) 120, 209, 311, 542
MboI GATC 4 cut(s) 118, 207, 309, 540
MboII GAAGA 4 cut(s) 123, 190, 203, 310
MhlI GDGCHC 2 cut(s) 94, 549
MluCI AATT 1 cut(s) 450
MnlI CCTC 7 cut(s) 98, 101, 107, 187, 211, 319, 538
MroI TCCGGA 1 cut(s) 239
MseI TTAA 1 cut(s) 147
MslI CAYNNNNRTG 1 cut(s) 357
MspI CCGG 7 cut(s) 14, 67, 132, 143, 240, 264, 534
MspR9I CCNGG 2 cut(s) 264, 535
MwoI GCNNNNNNNGC 5 cut(s) 19, 28, 284, 478, 530
NciI CCSGG 2 cut(s) 264, 535
NdeII GATC 4 cut(s) 118, 207, 309, 540
NlaIII CATG 1 cut(s) 479
NlaIV GGNNCC 3 cut(s) 43, 330, 547
NmeAIII GCCGAG 1 cut(s) 509
OliI CACNNNNGTG 1 cut(s) 357
PfeI GAWTC 2 cut(s) 54, 165
PinAI ACCGGT 1 cut(s) 142
PkrI GCNGC 5 cut(s) 162, 286, 350, 459, 526
PmaCI CACGTG 1 cut(s) 124
PmlI CACGTG 1 cut(s) 124
Ppu21I YACGTR 1 cut(s) 124
PpuMI RGGWCCY 1 cut(s) 328
Psp5II RGGWCCY 1 cut(s) 328
PspCI CACGTG 1 cut(s) 124
PspN4I GGNNCC 3 cut(s) 43, 330, 547
PspOMI GGGCCC 1 cut(s) 545
PspPI GGNCC 7 cut(s) 328, 346, 366, 379, 442, 545, 546
PspPPI RGGWCCY 1 cut(s) 328
RseI CAYNNNNRTG 1 cut(s) 357
SaqAI TTAA 1 cut(s) 147
SatI GCNGC 5 cut(s) 161, 285, 349, 458, 525
Sau3AI GATC 4 cut(s) 118, 207, 309, 540
Sau96I GGNCC 7 cut(s) 328, 346, 366, 379, 442, 545, 546
ScrFI CCNGG 2 cut(s) 264, 535
SduI GDGCHC 2 cut(s) 94, 549
SetI ASST 4 cut(s) 24, 126, 255, 554
SinI GGWCC 1 cut(s) 328
SmiMI CAYNNNNRTG 1 cut(s) 357
Sse9I AATT 1 cut(s) 450
SsiI CCGC 6 cut(s) 153, 160, 285, 349, 401, 525
SspMI CTAG 3 cut(s) 95, 275, 383
StyD4I CCNGG 2 cut(s) 262, 533
StyI CCWWGG 1 cut(s) 445
TaiI ACGT 1 cut(s) 126
TaqI TCGA 2 cut(s) 52, 79
TasI AATT 1 cut(s) 450
TauI GCSGC 4 cut(s) 163, 287, 351, 527
TfiI GAWTC 2 cut(s) 54, 165
Tru1I TTAA 1 cut(s) 147
Tru9I TTAA 1 cut(s) 147
TscAI CASTG 1 cut(s) 424
TseI GCWGC 1 cut(s) 457
TspDTI ATGAA 2 cut(s) 464, 534
TspRI CASTG 1 cut(s) 424
VpaK11BI GGWCC 1 cut(s) 328
XcmI CCANNNNNNNNNTGG 1 cut(s) 264
XspI CTAG 3 cut(s) 95, 275, 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.