pycom03g03350

PRA1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0016200
Physical Location & Seq
Reverse (-)
2638967 .. 2639628
662 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g03350.3

Sequence Viewer

Length: 516 bp
ATGGAATCAAAACCCATCAAACATTCAAACTCCCACTCCAAAAAATGTCGCCGTCAAAGCCAGTCACACTCCGTCACAGCAACAACCTATACCAGCATACCCATTTCCGTCTCGGGCTCCGACGCCATCACCCGATCCATCAAAAACCTCAACGACGCCGTTTCCCGCCGCCGCCCTTGGTCCGAATTCGCCTCCTCGATCTCCCGACTCGACTCCTTATCCTCCGCCCTGACCCGTATCCAGACCAACGCCAACCACTTCCGGGTCAATTACTTGGAGGACCCACTTGAGGTGTGGGGTCACCACGTCAGCGATTGGGGCGTGATGGCGGGTCTGGTGGTTGTTTCTGGTTTGGTTGCTTGGGCGAGCAGTGGGCTTGTGAACGTTACATTGGGCCTCGGAGTTGGGCTTGTGTTGTGTGGGGTCCACGGGCTGCTTCGGAACACAGAAGGCTTGTTTCTCAACGAGAATGATGCTTCTCAGGGATTGATTGGGCCCACATCTTCATCAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006810 GO:0008150 GO:0012505 GO:0016192 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

172

Amino Acids

18.35

Weight (kDa)

9.14

Isoelectric Point (pI)

39.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016200)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56230
fragaria_vesca FvH4_3g41570
malus_domestica MD03G1043200.v1.1 MD03G1043300.v1.1 MD11G1046400.v1.1
prunus_persica Prupe.6G035700_v2.0.a1
pyrus_communis pycom03g03350 pycom11g03780
rosa_chinensis RchiOBHm_Chr5g0074561
rosa_laevigata RLG00000036487
rosa_roxburghii Rroxscaffold_1G00007000
rosa_rugosa Rorug05G0432700
rosa_samantha Rh5BG510700 Rh5CG534500
rosa_wichuraiana Rw5G045410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 166, 169, 172, 225, 329
AclI AACGTT 1 cut(s) 384
AclWI GGATC 1 cut(s) 129
AcsI RAATTY 1 cut(s) 185
AcyI GRCGYC 2 cut(s) 123, 156
AfiI CCNNNNNNNGG 2 cut(s) 262, 289
AgsI TTSAA 1 cut(s) 27
AjiI CACGTC 1 cut(s) 307
AjuI GAANNNNNNNTTGG 2 cut(s) 374, 406
Alw26I GTCTC 1 cut(s) 115
AlwI GGATC 1 cut(s) 129
Ama87I CYCGRG 1 cut(s) 112
AoxI GGCC 2 cut(s) 394, 494
ApaI GGGCCC 1 cut(s) 498
ApeKI GCWGC 1 cut(s) 433
ApoI RAATTY 1 cut(s) 185
AspS9I GGNCC 6 cut(s) 180, 280, 394, 424, 494, 495
AsuC2I CCSGG 1 cut(s) 263
AsuHPI GGTGA 2 cut(s) 121, 293
AvaI CYCGRG 1 cut(s) 112
AvaII GGWCC 3 cut(s) 180, 280, 424
BaeGI GKGCMC 1 cut(s) 498
BanII GRGCYC 2 cut(s) 119, 498
BbvI GCAGC 1 cut(s) 420
BccI CCATC 4 cut(s) 23, 134, 146, 319
BceAI ACGGC 2 cut(s) 36, 143
BcgI CGANNNNNNTGC 2 cut(s) 455, 489
BciVI GTATCC 1 cut(s) 248
BcnI CCSGG 1 cut(s) 263
BcoDI GTCTC 1 cut(s) 115
BfuI GTATCC 1 cut(s) 248
BisI GCNGC 3 cut(s) 169, 172, 434
BlsI GCNGC 3 cut(s) 170, 173, 435
Bme1390I CCNGG 1 cut(s) 263
Bme18I GGWCC 3 cut(s) 180, 280, 424
BmeT110I CYCGRG 1 cut(s) 112
BmgBI CACGTC 1 cut(s) 307
BmgT120I GGNCC 6 cut(s) 180, 280, 394, 424, 494, 495
BmiI GGNNCC 4 cut(s) 118, 282, 425, 496
BmrFI CCNGG 1 cut(s) 263
BmsI GCATC 1 cut(s) 463
BpuEI CTTGAG 1 cut(s) 308
BpuMI CCSGG 1 cut(s) 263
BsaHI GRCGYC 2 cut(s) 123, 156
BsaJI CCNNGG 3 cut(s) 176, 397, 427
BsaXI ACNNNNNCTCC 2 cut(s) 20, 50
Bsc4I CCNNNNNNNGG 2 cut(s) 262, 289
Bse1I ACTGG 1 cut(s) 61
BseDI CCNNGG 3 cut(s) 176, 397, 427
BseLI CCNNNNNNNGG 2 cut(s) 262, 289
BseMII CTCAG 1 cut(s) 494
BseNI ACTGG 1 cut(s) 61
BseRI GAGGAG 1 cut(s) 184
BseSI GKGCMC 1 cut(s) 498
BseXI GCAGC 1 cut(s) 420
BshFI GGCC 2 cut(s) 396, 496
BsiHKCI CYCGRG 1 cut(s) 112
BsiSI CCGG 1 cut(s) 262
BslI CCNNNNNNNGG 2 cut(s) 262, 289
BsmAI GTCTC 1 cut(s) 115
BsmBI CGTCTC 1 cut(s) 115
BsnI GGCC 2 cut(s) 396, 496
BsoBI CYCGRG 1 cut(s) 112
Bsp120I GGGCCC 1 cut(s) 494
Bsp1286I GDGCHC 2 cut(s) 119, 498
Bsp143I GATC 2 cut(s) 134, 198
BspACI CCGC 5 cut(s) 166, 169, 172, 225, 329
BspANI GGCC 2 cut(s) 396, 496
BspCNI CTCAG 1 cut(s) 493
BspLI GGNNCC 4 cut(s) 118, 282, 425, 496
BspPI GGATC 1 cut(s) 129
BsrI ACTGG 1 cut(s) 61
BssECI CCNNGG 3 cut(s) 176, 397, 427
BssMI GATC 2 cut(s) 134, 198
BssNI GRCGYC 2 cut(s) 123, 156
BssT1I CCWWGG 1 cut(s) 176
BstACI GRCGYC 2 cut(s) 123, 156
BstC8I GCNNGC 1 cut(s) 367
BstDEI CTNAG 1 cut(s) 480
BstDSI CCRYGG 1 cut(s) 427
BstEII GGTNACC 1 cut(s) 299
BstKTI GATC 2 cut(s) 137, 201
BstMAI GTCTC 1 cut(s) 115
BstMBI GATC 2 cut(s) 134, 198
BstMWI GCNNNNNNNGC 2 cut(s) 57, 318
BstPI GGTNACC 1 cut(s) 299
BstSCI CCNGG 1 cut(s) 261
BstSLI GKGCMC 1 cut(s) 498
BstV1I GCAGC 1 cut(s) 420
BsuI GTATCC 1 cut(s) 248
BsuRI GGCC 2 cut(s) 396, 496
BtgI CCRYGG 1 cut(s) 427
BtrI CACGTC 1 cut(s) 307
BtsI GCAGTG 1 cut(s) 376
BtsIMutI CAGTG 1 cut(s) 376
Cac8I GCNNGC 1 cut(s) 367
Cfr13I GGNCC 6 cut(s) 180, 280, 394, 424, 494, 495
CseI GACGC 2 cut(s) 131, 164
CviJI RGCY 8 cut(s) 60, 117, 376, 396, 409, 433, 453, 496
CviKI_1 RGCY 8 cut(s) 60, 117, 376, 396, 409, 433, 453, 496
DdeI CTNAG 1 cut(s) 480
DpnI GATC 2 cut(s) 136, 200
DpnII GATC 2 cut(s) 134, 198
EciI GGCGGA 1 cut(s) 214
Eco130I CCWWGG 1 cut(s) 176
Eco24I GRGCYC 2 cut(s) 119, 498
Eco47I GGWCC 3 cut(s) 180, 280, 424
Eco88I CYCGRG 1 cut(s) 112
Eco91I GGTNACC 1 cut(s) 299
EcoO109I RGGNCCY 1 cut(s) 280
EcoO65I GGTNACC 1 cut(s) 299
EcoRI GAATTC 1 cut(s) 185
EcoT14I CCWWGG 1 cut(s) 176
EcoT38I GRGCYC 2 cut(s) 119, 498
ErhI CCWWGG 1 cut(s) 176
Esp3I CGTCTC 1 cut(s) 115
FaiI YATR 2 cut(s) 90, 98
FauI CCCGC 2 cut(s) 173, 322
Fnu4HI GCNGC 3 cut(s) 169, 172, 434
FriOI GRGCYC 2 cut(s) 119, 498
Fsp4HI GCNGC 3 cut(s) 169, 172, 434
GluI GCNGC 3 cut(s) 169, 172, 434
HaeIII GGCC 2 cut(s) 396, 496
HapII CCGG 1 cut(s) 262
HgaI GACGC 2 cut(s) 131, 164
Hin1I GRCGYC 2 cut(s) 123, 156
HinfI GANTC 3 cut(s) 5, 207, 212
HpaII CCGG 1 cut(s) 262
HphI GGTGA 2 cut(s) 121, 293
Hpy166II GTNNAC 2 cut(s) 382, 427
Hpy188I TCNGA 5 cut(s) 121, 184, 401, 441, 515
Hpy188III TCNNGA 2 cut(s) 204, 241
Hpy8I GTNNAC 2 cut(s) 382, 427
Hpy99I CGWCG 2 cut(s) 125, 158
HpyAV CCTTC 1 cut(s) 443
HpyCH4IV ACGT 2 cut(s) 306, 384
HpyF10VI GCNNNNNNNGC 2 cut(s) 57, 318
HpyF3I CTNAG 1 cut(s) 480
HpySE526I ACGT 2 cut(s) 306, 384
Hsp92I GRCGYC 2 cut(s) 123, 156
Kzo9I GATC 2 cut(s) 134, 198
LmnI GCTCC 1 cut(s) 122
LpnPI CCDG 8 cut(s) 74, 106, 242, 254, 275, 320, 333, 467
Lsp1109I GCAGC 1 cut(s) 420
LweI GCATC 1 cut(s) 463
MaeII ACGT 2 cut(s) 306, 384
MaeIII GTNAC 4 cut(s) 63, 73, 299, 385
MalI GATC 2 cut(s) 136, 200
MboI GATC 2 cut(s) 134, 198
MboII GAAGA 1 cut(s) 495
MhlI GDGCHC 2 cut(s) 119, 498
MluCI AATT 2 cut(s) 185, 268
MlyI GAGTC 2 cut(s) 201, 206
MmeI TCCRAC 1 cut(s) 144
MnlI CCTC 7 cut(s) 158, 202, 205, 232, 271, 283, 407
MspI CCGG 1 cut(s) 262
MspR9I CCNGG 1 cut(s) 263
MwoI GCNNNNNNNGC 2 cut(s) 57, 318
NciI CCSGG 1 cut(s) 263
NdeII GATC 2 cut(s) 134, 198
NlaIV GGNNCC 4 cut(s) 118, 282, 425, 496
NmuCI GTSAC 3 cut(s) 63, 73, 299
PfeI GAWTC 1 cut(s) 5
PkrI GCNGC 3 cut(s) 170, 173, 435
PleI GAGTC 2 cut(s) 201, 206
PpsI GAGTC 2 cut(s) 201, 206
PpuMI RGGWCCY 1 cut(s) 280
Psp1406I AACGTT 1 cut(s) 384
Psp5II RGGWCCY 1 cut(s) 280
PspEI GGTNACC 1 cut(s) 299
PspN4I GGNNCC 4 cut(s) 118, 282, 425, 496
PspOMI GGGCCC 1 cut(s) 494
PspPI GGNCC 6 cut(s) 180, 280, 394, 424, 494, 495
PspPPI RGGWCCY 1 cut(s) 280
SatI GCNGC 3 cut(s) 169, 172, 434
Sau3AI GATC 2 cut(s) 134, 198
Sau96I GGNCC 6 cut(s) 180, 280, 394, 424, 494, 495
SchI GAGTC 2 cut(s) 201, 206
ScrFI CCNGG 1 cut(s) 263
SduI GDGCHC 2 cut(s) 119, 498
SetI ASST 5 cut(s) 89, 150, 294, 309, 387
SfaNI GCATC 1 cut(s) 463
SinI GGWCC 3 cut(s) 180, 280, 424
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
Sse9I AATT 2 cut(s) 185, 268
SsiI CCGC 5 cut(s) 166, 169, 172, 225, 329
StyD4I CCNGG 1 cut(s) 261
StyI CCWWGG 1 cut(s) 176
TaiI ACGT 2 cut(s) 309, 387
TaqI TCGA 2 cut(s) 197, 210
TasI AATT 2 cut(s) 185, 268
TauI GCSGC 2 cut(s) 171, 174
TfiI GAWTC 1 cut(s) 5
TscAI CASTG 1 cut(s) 376
TseFI GTSAC 3 cut(s) 63, 73, 299
TseI GCWGC 1 cut(s) 433
Tsp45I GTSAC 3 cut(s) 63, 73, 299
TspDTI ATGAA 1 cut(s) 495
TspGWI ACGGA 2 cut(s) 61, 97
TspRI CASTG 1 cut(s) 376
VpaK11BI GGWCC 3 cut(s) 180, 280, 424
XapI RAATTY 1 cut(s) 185
XcmI CCANNNNNNNNNTGG 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.