Rw5G045410

PRA1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0016200
Physical Location & Seq
Forward (+)
79389919 .. 79390787
869 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G045410.1

Sequence Viewer

Length: 579 bp
ATGTCGCAGTCGCAGCCGCAGCCGCAAACCGCCACTGCGACCTACACGAGCATCCCCGTCACCATTCCCGCCGCTGACGTCATCTCCCGATCCATCCAGAACCTTCTTGGCGCCATCTCCCGCCACCGCCCCTGGCCCGAGTTCGGATCCTCTCTAAGTCGACCCGACTCCTACTCCTCCGCCCTGACCCGACTCCGCTTCAACTCCCGCCACTTCCGCACCAACTACATTCTCCTCATCGGGGTCTGCGGCTTCCTCTCCCTCATCGGAAGCCCCAGCTGGCTTCTCGCGACGGCCGCCGTCGTCGGGCTGTGGCTGGTGATCTACTTCTTCAGGGAGGACCCACTCGTGGTGTTGGGCCGCCACTTTAGTGATTGGTTCGTGTTTGTGGGCCTGGTGCTGGTTTCTGTTGCGGTTGTGTGGTTGAGCAGTGGGCTTGGGAATGTGGTGTTGGGCCTTGGAATTGGGCTGCTCATTTGTGGGCTTCACGGGCTGGTTCGGGATACCGAAGGCTTGTTTCTCAACGAGAATGAAGCCGCCTCGGCCGGGTTGATCGGGCCCACCTATACCACCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006810 GO:0008150 GO:0012505 GO:0016192 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

192

Amino Acids

20.77

Weight (kDa)

7.87

Isoelectric Point (pI)

41.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 39 - 177 3.9e-37 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016200)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56230
fragaria_vesca FvH4_3g41570
malus_domestica MD03G1043200.v1.1 MD03G1043300.v1.1 MD11G1046400.v1.1
prunus_persica Prupe.6G035700_v2.0.a1
pyrus_communis pycom03g03350 pycom11g03780
rosa_chinensis RchiOBHm_Chr5g0074561
rosa_laevigata RLG00000036487
rosa_roxburghii Rroxscaffold_1G00007000
rosa_rugosa Rorug05G0432700
rosa_samantha Rh5BG510700 Rh5CG534500
rosa_wichuraiana Rw5G045410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 81
AccB1I GGYRCC 1 cut(s) 110
AccI GTMKAC 1 cut(s) 160
AccII CGCG 1 cut(s) 290
AclWI GGATC 3 cut(s) 84, 141, 154
AcoI YGGCCR 2 cut(s) 294, 543
AcuI CTGAAG 1 cut(s) 316
AcyI GRCGYC 2 cut(s) 78, 111
AfiI CCNNNNNNNGG 6 cut(s) 143, 241, 306, 349, 400, 546
AgsI TTSAA 1 cut(s) 202
AjnI CCWGG 2 cut(s) 131, 393
AjuI GAANNNNNNNTTGG 2 cut(s) 434, 466
AleI CACNNNNGTG 1 cut(s) 369
AluBI AGCT 1 cut(s) 279
AluI AGCT 1 cut(s) 279
AlwI GGATC 3 cut(s) 84, 141, 154
Ama87I CYCGRG 1 cut(s) 137
AoxI GGCC 7 cut(s) 134, 294, 358, 391, 454, 543, 557
ApaI GGGCCC 1 cut(s) 561
ApeKI GCWGC 3 cut(s) 13, 19, 469
AspLEI GCGC 1 cut(s) 113
AspS9I GGNCC 7 cut(s) 135, 340, 358, 391, 454, 557, 558
AsuC2I CCSGG 1 cut(s) 547
AsuHPI GGTGA 2 cut(s) 52, 331
AvaI CYCGRG 1 cut(s) 137
AvaII GGWCC 1 cut(s) 340
BaeGI GKGCMC 1 cut(s) 561
BamHI GGATCC 1 cut(s) 146
BanI GGYRCC 1 cut(s) 110
BanII GRGCYC 1 cut(s) 561
BauI CACGAG 2 cut(s) 46, 347
BbvI GCAGC 3 cut(s) 25, 31, 456
BccI CCATC 2 cut(s) 101, 122
BceAI ACGGC 2 cut(s) 284, 309
BciT130I CCWGG 2 cut(s) 133, 395
BciVI GTATCC 1 cut(s) 496
BcnI CCSGG 1 cut(s) 547
BfoI RGCGCY 1 cut(s) 114
BfuI GTATCC 1 cut(s) 496
BglI GCCNNNNNGGC 1 cut(s) 542
Bme1390I CCNGG 3 cut(s) 133, 395, 547
Bme18I GGWCC 1 cut(s) 340
BmeT110I CYCGRG 1 cut(s) 137
BmgT120I GGNCC 7 cut(s) 135, 340, 358, 391, 454, 557, 558
BmiI GGNNCC 4 cut(s) 112, 148, 342, 559
BmrFI CCNGG 3 cut(s) 133, 395, 547
BmsI GCATC 1 cut(s) 60
BpuMI CCSGG 1 cut(s) 547
BsaHI GRCGYC 2 cut(s) 78, 111
BsaJI CCNNGG 3 cut(s) 131, 457, 540
BsaXI ACNNNNNCTCC 4 cut(s) 68, 98, 158, 188
Bsc4I CCNNNNNNNGG 6 cut(s) 143, 241, 306, 349, 400, 546
BseBI CCWGG 2 cut(s) 133, 395
BseDI CCNNGG 3 cut(s) 131, 457, 540
BseGI GGATG 2 cut(s) 51, 93
BseLI CCNNNNNNNGG 6 cut(s) 143, 241, 306, 349, 400, 546
BseRI GAGGAG 2 cut(s) 166, 224
BseSI GKGCMC 1 cut(s) 561
BseX3I CGGCCG 2 cut(s) 294, 543
BseXI GCAGC 3 cut(s) 25, 31, 456
BseYI CCCAGC 1 cut(s) 275
Bsh1236I CGCG 1 cut(s) 290
Bsh1285I CGRYCG 2 cut(s) 297, 546
BshFI GGCC 7 cut(s) 136, 296, 360, 393, 456, 545, 559
BshNI GGYRCC 1 cut(s) 110
BsiEI CGRYCG 2 cut(s) 297, 546
BsiHKCI CYCGRG 1 cut(s) 137
BsiSI CCGG 1 cut(s) 546
BslI CCNNNNNNNGG 6 cut(s) 143, 241, 306, 349, 400, 546
BsnI GGCC 7 cut(s) 136, 296, 360, 393, 456, 545, 559
BsoBI CYCGRG 1 cut(s) 137
Bsp120I GGGCCC 1 cut(s) 557
Bsp1286I GDGCHC 1 cut(s) 561
Bsp143I GATC 4 cut(s) 89, 146, 321, 552
Bsp68I TCGCGA 1 cut(s) 290
BspANI GGCC 7 cut(s) 136, 296, 360, 393, 456, 545, 559
BspFNI CGCG 1 cut(s) 290
BspLI GGNNCC 4 cut(s) 112, 148, 342, 559
BspPI GGATC 3 cut(s) 84, 141, 154
BspT107I GGYRCC 1 cut(s) 110
BssECI CCNNGG 3 cut(s) 131, 457, 540
BssMI GATC 4 cut(s) 89, 146, 321, 552
BssNI GRCGYC 2 cut(s) 78, 111
BssSI CACGAG 2 cut(s) 46, 347
BssT1I CCWWGG 1 cut(s) 457
Bst2BI CACGAG 2 cut(s) 46, 347
Bst2UI CCWGG 2 cut(s) 133, 395
BstACI GRCGYC 2 cut(s) 78, 111
BstC8I GCNNGC 1 cut(s) 281
BstDEI CTNAG 1 cut(s) 155
BstF5I GGATG 2 cut(s) 51, 93
BstFNI CGCG 1 cut(s) 290
BstH2I RGCGCY 1 cut(s) 114
BstHHI GCGC 1 cut(s) 113
BstKTI GATC 4 cut(s) 92, 149, 324, 555
BstMBI GATC 4 cut(s) 89, 146, 321, 552
BstMCI CGRYCG 2 cut(s) 297, 546
BstMWI GCNNNNNNNGC 7 cut(s) 13, 19, 22, 216, 296, 490, 542
BstNI CCWGG 2 cut(s) 133, 395
BstSCI CCNGG 3 cut(s) 131, 393, 545
BstSLI GKGCMC 1 cut(s) 561
BstUI CGCG 1 cut(s) 290
BstV1I GCAGC 3 cut(s) 25, 31, 456
BstX2I RGATCY 1 cut(s) 146
BstYI RGATCY 1 cut(s) 146
BstZI CGGCCG 2 cut(s) 294, 543
BsuI GTATCC 1 cut(s) 496
BsuRI GGCC 7 cut(s) 136, 296, 360, 393, 456, 545, 559
BtsCI GGATG 2 cut(s) 51, 93
BtsI GCAGTG 2 cut(s) 33, 436
BtsIMutI CAGTG 2 cut(s) 33, 436
BtuMI TCGCGA 1 cut(s) 290
Cac8I GCNNGC 1 cut(s) 281
CfoI GCGC 1 cut(s) 113
Cfr13I GGNCC 7 cut(s) 135, 340, 358, 391, 454, 557, 558
DdeI CTNAG 1 cut(s) 155
DinI GGCGCC 1 cut(s) 112
DpnI GATC 4 cut(s) 91, 148, 323, 554
DpnII GATC 4 cut(s) 89, 146, 321, 552
EaeI YGGCCR 2 cut(s) 294, 543
EagI CGGCCG 2 cut(s) 294, 543
EciI GGCGGA 1 cut(s) 169
EclXI CGGCCG 2 cut(s) 294, 543
Eco130I CCWWGG 1 cut(s) 457
Eco24I GRGCYC 1 cut(s) 561
Eco47I GGWCC 1 cut(s) 340
Eco52I CGGCCG 2 cut(s) 294, 543
Eco57I CTGAAG 1 cut(s) 316
Eco88I CYCGRG 1 cut(s) 137
EcoO109I RGGNCCY 1 cut(s) 340
EcoRII CCWGG 2 cut(s) 131, 393
EcoT14I CCWWGG 1 cut(s) 457
EcoT38I GRGCYC 1 cut(s) 561
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 457
FaiI YATR 1 cut(s) 567
FauI CCCGC 3 cut(s) 76, 128, 215
FblI GTMKAC 1 cut(s) 160
FokI GGATG 2 cut(s) 38, 80
FriOI GRGCYC 1 cut(s) 561
GlaI GCGC 1 cut(s) 112
GsaI CCCAGC 1 cut(s) 279
HaeII RGCGCY 1 cut(s) 114
HaeIII GGCC 7 cut(s) 136, 296, 360, 393, 456, 545, 559
HapII CCGG 1 cut(s) 546
HhaI GCGC 1 cut(s) 113
Hin1I GRCGYC 2 cut(s) 78, 111
Hin6I GCGC 1 cut(s) 111
HinP1I GCGC 1 cut(s) 111
HincII GTYRAC 1 cut(s) 161
HindII GTYRAC 1 cut(s) 161
HinfI GANTC 2 cut(s) 167, 192
HpaII CCGG 1 cut(s) 546
HphI GGTGA 2 cut(s) 52, 331
Hpy166II GTNNAC 1 cut(s) 161
Hpy188I TCNGA 3 cut(s) 146, 269, 578
Hpy188III TCNNGA 4 cut(s) 87, 97, 289, 500
Hpy8I GTNNAC 1 cut(s) 161
Hpy99I CGWCG 3 cut(s) 295, 305, 308
HpyAV CCTTC 2 cut(s) 113, 503
HpyCH4IV ACGT 1 cut(s) 78
HpyF10VI GCNNNNNNNGC 7 cut(s) 13, 19, 22, 216, 296, 490, 542
HpyF3I CTNAG 1 cut(s) 155
HpySE526I ACGT 1 cut(s) 78
Hsp92I GRCGYC 2 cut(s) 78, 111
HspAI GCGC 1 cut(s) 111
KasI GGCGCC 1 cut(s) 110
Kzo9I GATC 4 cut(s) 89, 146, 321, 552
Lsp1109I GCAGC 3 cut(s) 25, 31, 456
LweI GCATC 1 cut(s) 60
MaeII ACGT 1 cut(s) 78
MaeIII GTNAC 1 cut(s) 58
MalI GATC 4 cut(s) 91, 148, 323, 554
MboI GATC 4 cut(s) 89, 146, 321, 552
MboII GAAGA 1 cut(s) 322
MflI RGATCY 1 cut(s) 146
MhlI GDGCHC 1 cut(s) 561
MluCI AATT 1 cut(s) 462
Mly113I GGCGCC 1 cut(s) 111
MlyI GAGTC 2 cut(s) 161, 186
MnlI CCTC 7 cut(s) 160, 187, 245, 266, 272, 331, 550
MslI CAYNNNNRTG 1 cut(s) 369
MspA1I CMGCKG 2 cut(s) 74, 279
MspI CCGG 1 cut(s) 546
MspR9I CCNGG 3 cut(s) 133, 395, 547
MvaI CCWGG 2 cut(s) 133, 395
MvnI CGCG 1 cut(s) 290
MwoI GCNNNNNNNGC 7 cut(s) 13, 19, 22, 216, 296, 490, 542
NarI GGCGCC 1 cut(s) 111
NciI CCSGG 1 cut(s) 547
NdeII GATC 4 cut(s) 89, 146, 321, 552
NlaIV GGNNCC 4 cut(s) 112, 148, 342, 559
NmeAIII GCCGAG 1 cut(s) 521
NmuCI GTSAC 1 cut(s) 58
NruI TCGCGA 1 cut(s) 290
OliI CACNNNNGTG 1 cut(s) 369
PleI GAGTC 2 cut(s) 161, 186
PluTI GGCGCC 1 cut(s) 114
PpsI GAGTC 2 cut(s) 161, 186
PpuMI RGGWCCY 1 cut(s) 340
Psp5II RGGWCCY 1 cut(s) 340
Psp6I CCWGG 2 cut(s) 131, 393
PspFI CCCAGC 1 cut(s) 275
PspGI CCWGG 2 cut(s) 131, 393
PspN4I GGNNCC 4 cut(s) 112, 148, 342, 559
PspOMI GGGCCC 1 cut(s) 557
PspPI GGNCC 7 cut(s) 135, 340, 358, 391, 454, 557, 558
PspPPI RGGWCCY 1 cut(s) 340
PsuI RGATCY 1 cut(s) 146
PvuII CAGCTG 1 cut(s) 279
RruI TCGCGA 1 cut(s) 290
RseI CAYNNNNRTG 1 cut(s) 369
SalI GTCGAC 1 cut(s) 159
Sau3AI GATC 4 cut(s) 89, 146, 321, 552
Sau96I GGNCC 7 cut(s) 135, 340, 358, 391, 454, 557, 558
SchI GAGTC 2 cut(s) 161, 186
ScrFI CCNGG 3 cut(s) 133, 395, 547
SduI GDGCHC 1 cut(s) 561
SetI ASST 5 cut(s) 44, 81, 105, 281, 566
SfaNI GCATC 1 cut(s) 60
SfoI GGCGCC 1 cut(s) 112
SinI GGWCC 1 cut(s) 340
SmiMI CAYNNNNRTG 1 cut(s) 369
Sse9I AATT 1 cut(s) 462
SspDI GGCGCC 1 cut(s) 110
StyD4I CCNGG 3 cut(s) 131, 393, 545
StyI CCWWGG 1 cut(s) 457
TaiI ACGT 1 cut(s) 81
TaqI TCGA 1 cut(s) 160
TasI AATT 1 cut(s) 462
TauI GCSGC 7 cut(s) 19, 25, 74, 252, 299, 363, 539
TscAI CASTG 2 cut(s) 40, 436
TseFI GTSAC 1 cut(s) 58
TseI GCWGC 3 cut(s) 13, 19, 469
Tsp45I GTSAC 1 cut(s) 58
TspDTI ATGAA 1 cut(s) 546
TspRI CASTG 2 cut(s) 40, 436
VpaK11BI GGWCC 1 cut(s) 340
XcmI CCANNNNNNNNNTGG 1 cut(s) 104
XmiI GTMKAC 1 cut(s) 160
ZraI GACGTC 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.