Rorug05G0432700

PRA1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0016200
Physical Location & Seq
Reverse (-)
60179362 .. 60180813
1452 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0432700.1

Sequence Viewer

Length: 1452 bp
ATGGACGACAATGAAGACGAACAGACAAGTTCTCTTCCTCCCAAGAAGAGACTGAAACAACACCATCGTCGTCAGCCTAATCATGAAGGCAGCAGCAGTGAAATCCATTCTTGCAATGTCTGCCAGAAAAGCTTCGCATCACTCAAGGCTTTGAACGGCCACATGAAAATCCACGGCGGAAGCAAGTCCAAGGCTGCCAGGCCTCCGTTTCCGGCGCCGGAACCATCTGAAGACAAAGACGATGATGGTGTTAGTGCTAGTAGTTCATCCGATACCAACATAAAGAAAGATGTTGATGTAGCAAGCTTCTTGCAATACTTGAGCGATATCGAAATTCAGAGGAACGGGCCAAACGGGCCAAGGGAAGAAGGGTATTGTTGGAAGCAATGCAGTAGAGATTACAATGCAGAAGTCCGGCTTGGTTATGGAGTTTCAGGGAAGAAGGCAATGCCGGTTCCATACAAGGACAACCCTTCATCCAAGAAAGTGAAAATTGATGGTACAACTAGAACTGGTTCTGCTTTGGTACAGAAGAAGTTGGCTGGAGGTAAAAGAAAGGCTTCTGTGGAAGATGATGAGAAAGGTGATCAAAGCAGAGTACCAAAAGATTTCACAAGTAATATTTCAAAGAAGGTAAAGATTGTTGGCAGAGGTGAAGACCCCAAAAGAGACATGGTTTCACATGACCAATCCCGGAAGCTGCAGATTCCAAGAAAGCCCCAAGTTGAGTCAAGTAAAACCTTCCCAGCTTCACAAGCAACGGAGACTTTCAGCTGGAATTGTAAAAGTAGCAGATCGGCTAGTGCGAGTGTGAAAATAGGTGAATCTGGGTTTGGTACTAAGTATGCTGCAAAAAGTAGTACGAGTTTGAAAATAGGTGAATCTGGGTTTGGTGCTAAGTATGGAACAAAGAGTAATACGAGTGTAAAAATGGGTCATTCTGGGTTTGGTACCAAGTATGCAAAGAGTAATACGAGTGTAAAAGTGGGTGAATCTGGGTTTGGTACTAAGTTGGCTGCTCGTGCTCCTACTAGTACTAAAGAAGCTGCAGTGGAGGATAACAAACAGCATGTGTGCGAAATTTGCCACAGGACGTTTAAGACTTATCAGGGTCTTGGTGGCCACAGGAGGATTCATAATTCGAAAGGAGGAGTGGCGAAAGAAGCTCCTACAGCACCTGAGGTCAGAGCAGCGACAGGGCATGAAGATGGTGAGACTGGTGTAGACCCTCAAGGCCACAGGAGTGTTGGTAGTTCTAGAAGAGTATCAGTGAGAAAAGCTCCTGCTGCACCTGTGGTTAGAGCACCGTCTGATCATGAAGATGGTGGGAGTGATGAAGACCCTGAAGCAGCATTGCATGCAGAAGAAGCAGGGGCCAAAACTGAAGGGCGCCATGAAAGAGGTTTCAATCTCAACCAAGTCCCAGATGAGGATTCGGATGGAGCTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006810 GO:0008150 GO:0012505 GO:0016192 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

483

Amino Acids

51.9

Weight (kDa)

9.23

Isoelectric Point (pI)

50.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C2H2_6 PF13912 36 - 58 2.4e-07 C2H2-type zinc finger
zf-C2H2_6 PF13912 357 - 380 2.4e-07 C2H2-type zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016200)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56230
fragaria_vesca FvH4_3g41570
malus_domestica MD03G1043200.v1.1 MD03G1043300.v1.1 MD11G1046400.v1.1
prunus_persica Prupe.6G035700_v2.0.a1
pyrus_communis pycom03g03350 pycom11g03780
rosa_chinensis RchiOBHm_Chr5g0074561
rosa_laevigata RLG00000036487
rosa_roxburghii Rroxscaffold_1G00007000
rosa_rugosa Rorug05G0432700
rosa_samantha Rh5BG510700 Rh5CG534500
rosa_wichuraiana Rw5G045410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 950
AccB1I GGYRCC 3 cut(s) 214, 950, 1389
AccI GTMKAC 1 cut(s) 1224
AciI CCGC 1 cut(s) 177
AcoI YGGCCR 2 cut(s) 157, 1120
AcsI RAATTY 2 cut(s) 333, 1080
AcuI CTGAAG 3 cut(s) 249, 1365, 1404
AcyI GRCGYC 2 cut(s) 215, 1390
AfaI GTAC 8 cut(s) 502, 528, 600, 838, 862, 952, 1006, 1036
AfiI CCNNNNNNNGG 1 cut(s) 1429
AgsI TTSAA 4 cut(s) 154, 627, 871, 1408
AhlI ACTAGT 1 cut(s) 1031
AjnI CCWGG 1 cut(s) 197
AjuI GAANNNNNNNTTGG 8 cut(s) 402, 434, 816, 848, 873, 905, 984, 1016
AleI CACNNNNGTG 1 cut(s) 1242
AluBI AGCT 9 cut(s) 132, 306, 700, 749, 774, 1046, 1166, 1280, 1445
AluI AGCT 9 cut(s) 132, 306, 700, 749, 774, 1046, 1166, 1280, 1445
Alw21I GWGCWC 2 cut(s) 1027, 1306
Alw26I GTCTC 4 cut(s) 43, 663, 758, 1208
AlwNI CAGNNNCTG 1 cut(s) 1178
AoxI GGCC 7 cut(s) 157, 200, 347, 356, 1120, 1234, 1374
ApoI RAATTY 2 cut(s) 333, 1080
Asp700I GAANNNNTTC 3 cut(s) 131, 514, 559
Asp718I GGTACC 1 cut(s) 950
AspLEI GCGC 2 cut(s) 217, 1392
AspS9I GGNCC 3 cut(s) 347, 356, 1374
AsuC2I CCSGG 1 cut(s) 694
AsuHPI GGTGA 6 cut(s) 596, 665, 833, 890, 1001, 1223
AsuII TTCGAA 1 cut(s) 1142
AxyI CCTNAGG 1 cut(s) 1179
BalI TGGCCA 1 cut(s) 1122
BanI GGYRCC 3 cut(s) 214, 950, 1389
BauI CACGAG 1 cut(s) 1020
BbsI GAAGAC 4 cut(s) 21, 237, 663, 1344
Bbv12I GWGCWC 2 cut(s) 1027, 1306
BccI CCATC 7 cut(s) 72, 232, 239, 491, 1202, 1316, 1433
BceAI ACGGC 2 cut(s) 172, 190
BciT130I CCWGG 1 cut(s) 199
BclI TGATCA 2 cut(s) 586, 1312
BcnI CCSGG 1 cut(s) 694
BcoDI GTCTC 4 cut(s) 43, 663, 758, 1208
BcuI ACTAGT 1 cut(s) 1031
BfaI CTAG 5 cut(s) 258, 507, 801, 1032, 1257
BfmI CTRYAG 3 cut(s) 701, 1047, 1170
BfoI RGCGCY 2 cut(s) 218, 1393
BglI GCCNNNNNGGC 1 cut(s) 355
BmcAI AGTACT 1 cut(s) 1036
Bme1390I CCNGG 2 cut(s) 199, 694
BmgT120I GGNCC 3 cut(s) 347, 356, 1374
BmiI GGNNCC 6 cut(s) 216, 222, 456, 952, 1375, 1391
BmrFI CCNGG 2 cut(s) 199, 694
BmsI GCATC 1 cut(s) 146
BpiI GAAGAC 4 cut(s) 21, 237, 663, 1344
BplI GAGNNNNNCTC 2 cut(s) 1264, 1296
BpmI CTGGAG 1 cut(s) 564
Bpu14I TTCGAA 1 cut(s) 1142
BpuEI CTTGAG 3 cut(s) 128, 340, 1215
BpuMI CCSGG 1 cut(s) 694
BsaHI GRCGYC 2 cut(s) 215, 1390
BsaJI CCNNGG 3 cut(s) 172, 189, 359
BsaXI ACNNNNNCTCC 2 cut(s) 1234, 1264
Bsc4I CCNNNNNNNGG 1 cut(s) 1429
Bse118I RCCGGY 1 cut(s) 451
Bse1I ACTGG 2 cut(s) 517, 1222
Bse21I CCTNAGG 1 cut(s) 1179
Bse3DI GCAATG 4 cut(s) 121, 392, 453, 1352
BseBI CCWGG 1 cut(s) 199
BseDI CCNNGG 3 cut(s) 172, 189, 359
BseGI GGATG 3 cut(s) 266, 476, 1444
BseLI CCNNNNNNNGG 1 cut(s) 1429
BseMI GCAATG 4 cut(s) 121, 392, 453, 1352
BseMII CTCAG 1 cut(s) 1170
BseNI ACTGG 2 cut(s) 517, 1222
BseRI GAGGAG 1 cut(s) 1164
BseYI CCCAGC 1 cut(s) 745
BsgI GTGCAG 1 cut(s) 1272
BshFI GGCC 7 cut(s) 159, 202, 349, 358, 1122, 1236, 1376
BshNI GGYRCC 3 cut(s) 214, 950, 1389
BsiHKAI GWGCWC 2 cut(s) 1027, 1306
BsiSI CCGG 5 cut(s) 212, 218, 415, 452, 694
BslFI GGGAC 1 cut(s) 1406
BslI CCNNNNNNNGG 1 cut(s) 1429
BsmAI GTCTC 4 cut(s) 43, 663, 758, 1208
BsmFI GGGAC 1 cut(s) 1406
BsnI GGCC 7 cut(s) 159, 202, 349, 358, 1122, 1236, 1376
Bsp119I TTCGAA 1 cut(s) 1142
Bsp1286I GDGCHC 2 cut(s) 1027, 1306
Bsp143I GATC 3 cut(s) 586, 794, 1312
BspACI CCGC 1 cut(s) 177
BspANI GGCC 7 cut(s) 159, 202, 349, 358, 1122, 1236, 1376
BspCNI CTCAG 1 cut(s) 1171
BspHI TCATGA 2 cut(s) 82, 1315
BspLI GGNNCC 6 cut(s) 216, 222, 456, 952, 1375, 1391
BspMAI CTGCAG 2 cut(s) 705, 1051
BspT104I TTCGAA 1 cut(s) 1142
BspT107I GGYRCC 3 cut(s) 214, 950, 1389
BsrDI GCAATG 4 cut(s) 121, 392, 453, 1352
BsrFI RCCGGY 1 cut(s) 451
BsrI ACTGG 2 cut(s) 517, 1222
BssAI RCCGGY 1 cut(s) 451
BssECI CCNNGG 3 cut(s) 172, 189, 359
BssMI GATC 3 cut(s) 586, 794, 1312
BssNI GRCGYC 2 cut(s) 215, 1390
BssSI CACGAG 1 cut(s) 1020
BssT1I CCWWGG 2 cut(s) 189, 359
Bst2BI CACGAG 1 cut(s) 1020
Bst2UI CCWGG 1 cut(s) 199
Bst4CI ACNGT 1 cut(s) 1308
Bst6I CTCTTC 3 cut(s) 39, 41, 1255
BstACI GRCGYC 2 cut(s) 215, 1390
BstAPI GCANNNNNTGC 2 cut(s) 120, 1358
BstBI TTCGAA 1 cut(s) 1142
BstC8I GCNNGC 2 cut(s) 304, 1359
BstDEI CTNAG 4 cut(s) 840, 897, 1008, 1179
BstDSI CCRYGG 1 cut(s) 172
BstF5I GGATG 3 cut(s) 266, 476, 1444
BstH2I RGCGCY 2 cut(s) 218, 1393
BstHHI GCGC 2 cut(s) 217, 1392
BstKTI GATC 3 cut(s) 589, 797, 1315
BstMAI GTCTC 4 cut(s) 43, 663, 758, 1208
BstMBI GATC 3 cut(s) 586, 794, 1312
BstNI CCWGG 1 cut(s) 199
BstNSI RCATGY 2 cut(s) 1073, 1361
BstSCI CCNGG 2 cut(s) 197, 692
BstSFI CTRYAG 3 cut(s) 701, 1047, 1170
BstV2I GAAGAC 4 cut(s) 21, 237, 663, 1344
Bsu36I CCTNAGG 1 cut(s) 1179
BsuRI GGCC 7 cut(s) 159, 202, 349, 358, 1122, 1236, 1376
BtgI CCRYGG 1 cut(s) 172
BtsCI GGATG 3 cut(s) 266, 476, 1444
BtsI GCAGTG 2 cut(s) 103, 1056
BtsIMutI CAGTG 3 cut(s) 103, 1056, 1275
Cac8I GCNNGC 2 cut(s) 304, 1359
CaiI CAGNNNCTG 1 cut(s) 1178
CciI TCATGA 2 cut(s) 82, 1315
CfoI GCGC 2 cut(s) 217, 1392
Cfr10I RCCGGY 1 cut(s) 451
Cfr13I GGNCC 3 cut(s) 347, 356, 1374
Csp6I GTAC 8 cut(s) 501, 527, 599, 837, 861, 951, 1005, 1035
CviAII CATG 9 cut(s) 83, 163, 673, 683, 1070, 1202, 1316, 1358, 1394
CviQI GTAC 8 cut(s) 501, 527, 599, 837, 861, 951, 1005, 1035
DdeI CTNAG 4 cut(s) 840, 897, 1008, 1179
DinI GGCGCC 2 cut(s) 216, 1391
DpnI GATC 3 cut(s) 588, 796, 1314
DpnII GATC 3 cut(s) 586, 794, 1312
EaeI YGGCCR 2 cut(s) 157, 1120
Eam1104I CTCTTC 3 cut(s) 39, 41, 1255
EarI CTCTTC 3 cut(s) 39, 41, 1255
EciI GGCGGA 1 cut(s) 192
Eco130I CCWWGG 2 cut(s) 189, 359
Eco147I AGGCCT 1 cut(s) 202
Eco32I GATATC 1 cut(s) 328
Eco57I CTGAAG 3 cut(s) 249, 1365, 1404
Eco81I CCTNAGG 1 cut(s) 1179
EcoRII CCWGG 1 cut(s) 197
EcoRV GATATC 1 cut(s) 328
EcoT14I CCWWGG 2 cut(s) 189, 359
EgeI GGCGCC 2 cut(s) 216, 1391
EheI GGCGCC 2 cut(s) 216, 1391
ErhI CCWWGG 2 cut(s) 189, 359
FaeI CATG 9 cut(s) 86, 166, 676, 686, 1073, 1205, 1319, 1361, 1397
FalI AAGNNNNNCTT 4 cut(s) 402, 434, 544, 576
FaqI GGGAC 1 cut(s) 1406
FatI CATG 9 cut(s) 82, 162, 672, 682, 1069, 1201, 1315, 1357, 1393
FbaI TGATCA 2 cut(s) 586, 1312
FblI GTMKAC 1 cut(s) 1224
FokI GGATG 2 cut(s) 253, 463
FspBI CTAG 5 cut(s) 258, 507, 801, 1032, 1257
GlaI GCGC 2 cut(s) 216, 1391
GsaI CCCAGC 1 cut(s) 749
GsuI CTGGAG 1 cut(s) 564
HaeII RGCGCY 2 cut(s) 218, 1393
HaeIII GGCC 7 cut(s) 159, 202, 349, 358, 1122, 1236, 1376
HapII CCGG 5 cut(s) 212, 218, 415, 452, 694
HhaI GCGC 2 cut(s) 217, 1392
Hin1I GRCGYC 2 cut(s) 215, 1390
Hin1II CATG 9 cut(s) 86, 166, 676, 686, 1073, 1205, 1319, 1361, 1397
Hin6I GCGC 2 cut(s) 215, 1390
HinP1I GCGC 2 cut(s) 215, 1390
HindIII AAGCTT 2 cut(s) 130, 304
HinfI GANTC 7 cut(s) 706, 728, 824, 881, 992, 1132, 1433
HpaII CCGG 5 cut(s) 212, 218, 415, 452, 694
HphI GGTGA 6 cut(s) 596, 665, 833, 890, 1001, 1223
Hpy166II GTNNAC 1 cut(s) 1225
Hpy188I TCNGA 6 cut(s) 229, 271, 339, 1187, 1312, 1438
Hpy188III TCNNGA 3 cut(s) 83, 1257, 1316
Hpy8I GTNNAC 1 cut(s) 1225
Hpy99I CGWCG 1 cut(s) 72
HpyAV CCTTC 7 cut(s) 80, 362, 436, 483, 625, 751, 1379
HpyCH4III ACNGT 1 cut(s) 1308
HpyCH4IV ACGT 1 cut(s) 1094
HpyF3I CTNAG 4 cut(s) 840, 897, 1008, 1179
HpySE526I ACGT 1 cut(s) 1094
Hsp92I GRCGYC 2 cut(s) 215, 1390
Hsp92II CATG 9 cut(s) 86, 166, 676, 686, 1073, 1205, 1319, 1361, 1397
HspAI GCGC 2 cut(s) 215, 1390
KasI GGCGCC 2 cut(s) 214, 1389
KpnI GGTACC 1 cut(s) 954
Ksp22I TGATCA 2 cut(s) 586, 1312
Kzo9I GATC 3 cut(s) 586, 794, 1312
LmnI GCTCC 4 cut(s) 1030, 1171, 1285, 1442
LweI GCATC 1 cut(s) 146
MaeI CTAG 5 cut(s) 258, 507, 801, 1032, 1257
MaeII ACGT 1 cut(s) 1094
MalI GATC 3 cut(s) 588, 796, 1314
MboI GATC 3 cut(s) 586, 794, 1312
MhlI GDGCHC 2 cut(s) 1027, 1306
MlsI TGGCCA 1 cut(s) 1122
MluCI AATT 5 cut(s) 333, 492, 778, 1080, 1138
MluNI TGGCCA 1 cut(s) 1122
Mly113I GGCGCC 2 cut(s) 215, 1390
MlyI GAGTC 1 cut(s) 737
MmeI TCCRAC 1 cut(s) 359
Mox20I TGGCCA 1 cut(s) 1122
MroXI GAANNNNTTC 3 cut(s) 131, 514, 559
MscI TGGCCA 1 cut(s) 1122
MseI TTAA 2 cut(s) 1098, 1450
MslI CAYNNNNRTG 3 cut(s) 1206, 1242, 1320
Msp20I TGGCCA 1 cut(s) 1122
MspA1I CMGCKG 1 cut(s) 774
MspI CCGG 5 cut(s) 212, 218, 415, 452, 694
MspR9I CCNGG 2 cut(s) 199, 694
MvaI CCWGG 1 cut(s) 199
NarI GGCGCC 2 cut(s) 215, 1390
NciI CCSGG 1 cut(s) 694
NdeII GATC 3 cut(s) 586, 794, 1312
NlaIII CATG 9 cut(s) 86, 166, 676, 686, 1073, 1205, 1319, 1361, 1397
NlaIV GGNNCC 6 cut(s) 216, 222, 456, 952, 1375, 1391
NspI RCATGY 2 cut(s) 1073, 1361
NspV TTCGAA 1 cut(s) 1142
OliI CACNNNNGTG 1 cut(s) 1242
PaeI GCATGC 1 cut(s) 1361
PagI TCATGA 2 cut(s) 82, 1315
PceI AGGCCT 1 cut(s) 202
PcsI WCGNNNNNNNCGW 1 cut(s) 803
PdmI GAANNNNTTC 3 cut(s) 131, 514, 559
PfeI GAWTC 6 cut(s) 706, 824, 881, 992, 1132, 1433
PfoI TCCNGGA 1 cut(s) 692
PleI GAGTC 1 cut(s) 736
PluTI GGCGCC 2 cut(s) 218, 1393
PpsI GAGTC 1 cut(s) 736
Psp6I CCWGG 1 cut(s) 197
PspFI CCCAGC 1 cut(s) 745
PspGI CCWGG 1 cut(s) 197
PspN4I GGNNCC 6 cut(s) 216, 222, 456, 952, 1375, 1391
PspPI GGNCC 3 cut(s) 347, 356, 1374
PstI CTGCAG 2 cut(s) 705, 1051
PstNI CAGNNNCTG 1 cut(s) 1178
PvuII CAGCTG 1 cut(s) 774
RsaI GTAC 8 cut(s) 502, 528, 600, 838, 862, 952, 1006, 1036
RsaNI GTAC 8 cut(s) 501, 527, 599, 837, 861, 951, 1005, 1035
RseI CAYNNNNRTG 3 cut(s) 1206, 1242, 1320
SaqAI TTAA 2 cut(s) 1098, 1450
Sau3AI GATC 3 cut(s) 586, 794, 1312
Sau96I GGNCC 3 cut(s) 347, 356, 1374
ScaI AGTACT 1 cut(s) 1036
SchI GAGTC 1 cut(s) 737
ScrFI CCNGG 2 cut(s) 199, 694
SduI GDGCHC 2 cut(s) 1027, 1306
SfaNI GCATC 1 cut(s) 146
SfcI CTRYAG 3 cut(s) 701, 1047, 1170
SfiI GGCCNNNNNGGCC 1 cut(s) 355
SfoI GGCGCC 2 cut(s) 216, 1391
SfuI TTCGAA 1 cut(s) 1142
SmiMI CAYNNNNRTG 3 cut(s) 1206, 1242, 1320
SmlI CTYRAG 3 cut(s) 143, 319, 1230
SmoI CTYRAG 3 cut(s) 143, 319, 1230
SpeI ACTAGT 1 cut(s) 1031
SphI GCATGC 1 cut(s) 1361
Sse9I AATT 5 cut(s) 333, 492, 778, 1080, 1138
SseBI AGGCCT 1 cut(s) 202
SsiI CCGC 1 cut(s) 177
SspDI GGCGCC 2 cut(s) 214, 1389
SspI AATATT 1 cut(s) 622
SspMI CTAG 5 cut(s) 258, 507, 801, 1032, 1257
StuI AGGCCT 1 cut(s) 202
StyD4I CCNGG 2 cut(s) 197, 692
StyI CCWWGG 2 cut(s) 189, 359
TaaI ACNGT 1 cut(s) 1308
TaiI ACGT 1 cut(s) 1097
TaqI TCGA 2 cut(s) 330, 1142
TasI AATT 5 cut(s) 333, 492, 778, 1080, 1138
TatI WGTACW 1 cut(s) 1034
TfiI GAWTC 6 cut(s) 706, 824, 881, 992, 1132, 1433
Tru1I TTAA 2 cut(s) 1098, 1450
Tru9I TTAA 2 cut(s) 1098, 1450
TscAI CASTG 3 cut(s) 103, 1056, 1275
TspGWI ACGGA 2 cut(s) 195, 776
TspRI CASTG 3 cut(s) 103, 1056, 1275
XapI RAATTY 2 cut(s) 333, 1080
XbaI TCTAGA 1 cut(s) 1256
XceI RCATGY 2 cut(s) 1073, 1361
XcmI CCANNNNNNNNNTGG 2 cut(s) 670, 1244
XmiI GTMKAC 1 cut(s) 1224
XmnI GAANNNNTTC 3 cut(s) 131, 514, 559
XspI CTAG 5 cut(s) 258, 507, 801, 1032, 1257
ZrmI AGTACT 1 cut(s) 1036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.