Rh5BG510700

PRA1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0016200
Physical Location & Seq
Forward (+)
81162189 .. 81163211
1023 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG510700.1

Sequence Viewer

Length: 591 bp
ATGTCGCAGTCGCAGCCGCAGCCGCAGCCGCAGCCGCAAACCGCCACTGCGACCTACACGAGCATCCCCGTCACCATTCCCGCCGCTGACGTCATCTCCCGATCCATCCAGAACCTTCTTGGCGCCATCTCCCGCCACCGCCCCTGGCCCGAGTTCGGATCCTCTCTAAGTCGACCCGACTCCTACTCTTCCGCCCTGACCCGACTCCGCTTCAACTCCCGCCACTTCCGCACCAACTACATTCTCCTCATCGGGGTCTGCGGCTTCCTCTCCCTCATCGGAAGCCCCGGCTGGCTTCTCGCGACGGCCGCCGTCGTCGGGCTGTGGCTGGTGATCTACTTCTTCAGGGAGGACCCACTCGTGGTGTTGGGCCGCCACTTTAGTGATTGGTCCGTGTTTGTGGGCCTGGTGCTGCTTTCTGTTGCGGTTGTGTGGTTGAGCAGTGGGCTTGGGAATGTGGTGTTGGGCCTTGGAATTGGGCTGCTCATTTGTGGGCTTCACGGGCTGGTTCGGGATACTGAAGGCTTGTTTCTCAACGAGAATGAAGCCGCCTCGGCCGGGTTGATCGGGCCCACCTATACCACCGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006810 GO:0008150 GO:0012505 GO:0016192 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

196

Amino Acids

21.14

Weight (kDa)

7.87

Isoelectric Point (pI)

44.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 43 - 181 9.9e-37 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016200)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56230
fragaria_vesca FvH4_3g41570
malus_domestica MD03G1043200.v1.1 MD03G1043300.v1.1 MD11G1046400.v1.1
prunus_persica Prupe.6G035700_v2.0.a1
pyrus_communis pycom03g03350 pycom11g03780
rosa_chinensis RchiOBHm_Chr5g0074561
rosa_laevigata RLG00000036487
rosa_roxburghii Rroxscaffold_1G00007000
rosa_rugosa Rorug05G0432700
rosa_samantha Rh5BG510700 Rh5CG534500
rosa_wichuraiana Rw5G045410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 93
AccB1I GGYRCC 1 cut(s) 122
AccI GTMKAC 1 cut(s) 172
AccII CGCG 1 cut(s) 302
AclWI GGATC 3 cut(s) 96, 153, 166
AcoI YGGCCR 2 cut(s) 306, 555
AcuI CTGAAG 2 cut(s) 328, 540
AcyI GRCGYC 2 cut(s) 90, 123
AfiI CCNNNNNNNGG 5 cut(s) 155, 253, 318, 361, 558
AgsI TTSAA 1 cut(s) 214
AjnI CCWGG 2 cut(s) 143, 405
AjuI GAANNNNNNNTTGG 2 cut(s) 446, 478
AleI CACNNNNGTG 1 cut(s) 381
AlwI GGATC 3 cut(s) 96, 153, 166
Ama87I CYCGRG 1 cut(s) 149
AoxI GGCC 7 cut(s) 146, 306, 370, 403, 466, 555, 569
ApaI GGGCCC 1 cut(s) 573
ApeKI GCWGC 6 cut(s) 13, 19, 25, 31, 412, 481
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 8 cut(s) 147, 352, 370, 390, 403, 466, 569, 570
AsuC2I CCSGG 2 cut(s) 288, 559
AsuHPI GGTGA 2 cut(s) 64, 343
AvaI CYCGRG 1 cut(s) 149
AvaII GGWCC 2 cut(s) 352, 390
BaeGI GKGCMC 1 cut(s) 573
BamHI GGATCC 1 cut(s) 158
BanI GGYRCC 1 cut(s) 122
BanII GRGCYC 1 cut(s) 573
BauI CACGAG 2 cut(s) 58, 359
BbvI GCAGC 6 cut(s) 25, 31, 37, 43, 399, 468
BccI CCATC 2 cut(s) 113, 134
BceAI ACGGC 2 cut(s) 296, 321
BciT130I CCWGG 2 cut(s) 145, 407
BciVI GTATCC 1 cut(s) 508
BcnI CCSGG 2 cut(s) 288, 559
BfoI RGCGCY 1 cut(s) 126
BfuI GTATCC 1 cut(s) 508
BglI GCCNNNNNGGC 1 cut(s) 554
Bme1390I CCNGG 4 cut(s) 145, 288, 407, 559
Bme18I GGWCC 2 cut(s) 352, 390
BmeT110I CYCGRG 1 cut(s) 149
BmgT120I GGNCC 8 cut(s) 147, 352, 370, 390, 403, 466, 569, 570
BmiI GGNNCC 4 cut(s) 124, 160, 354, 571
BmrFI CCNGG 4 cut(s) 145, 288, 407, 559
BmsI GCATC 1 cut(s) 72
BpuMI CCSGG 2 cut(s) 288, 559
BsaHI GRCGYC 2 cut(s) 90, 123
BsaJI CCNNGG 4 cut(s) 143, 286, 469, 552
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bsc4I CCNNNNNNNGG 5 cut(s) 155, 253, 318, 361, 558
BseBI CCWGG 2 cut(s) 145, 407
BseDI CCNNGG 4 cut(s) 143, 286, 469, 552
BseGI GGATG 2 cut(s) 63, 105
BseLI CCNNNNNNNGG 5 cut(s) 155, 253, 318, 361, 558
BseRI GAGGAG 1 cut(s) 236
BseSI GKGCMC 1 cut(s) 573
BseX3I CGGCCG 2 cut(s) 306, 555
BseXI GCAGC 6 cut(s) 25, 31, 37, 43, 399, 468
Bsh1236I CGCG 1 cut(s) 302
Bsh1285I CGRYCG 2 cut(s) 309, 558
BshFI GGCC 7 cut(s) 148, 308, 372, 405, 468, 557, 571
BshNI GGYRCC 1 cut(s) 122
BsiEI CGRYCG 2 cut(s) 309, 558
BsiHKCI CYCGRG 1 cut(s) 149
BsiSI CCGG 2 cut(s) 288, 558
BslI CCNNNNNNNGG 5 cut(s) 155, 253, 318, 361, 558
BsnI GGCC 7 cut(s) 148, 308, 372, 405, 468, 557, 571
BsoBI CYCGRG 1 cut(s) 149
Bsp120I GGGCCC 1 cut(s) 569
Bsp1286I GDGCHC 1 cut(s) 573
Bsp143I GATC 4 cut(s) 101, 158, 333, 564
Bsp68I TCGCGA 1 cut(s) 302
BspANI GGCC 7 cut(s) 148, 308, 372, 405, 468, 557, 571
BspFNI CGCG 1 cut(s) 302
BspLI GGNNCC 4 cut(s) 124, 160, 354, 571
BspPI GGATC 3 cut(s) 96, 153, 166
BspT107I GGYRCC 1 cut(s) 122
BssECI CCNNGG 4 cut(s) 143, 286, 469, 552
BssMI GATC 4 cut(s) 101, 158, 333, 564
BssNI GRCGYC 2 cut(s) 90, 123
BssSI CACGAG 2 cut(s) 58, 359
BssT1I CCWWGG 1 cut(s) 469
Bst2BI CACGAG 2 cut(s) 58, 359
Bst2UI CCWGG 2 cut(s) 145, 407
Bst4CI ACNGT 1 cut(s) 586
Bst6I CTCTTC 1 cut(s) 193
BstACI GRCGYC 2 cut(s) 90, 123
BstC8I GCNNGC 1 cut(s) 293
BstDEI CTNAG 1 cut(s) 167
BstF5I GGATG 2 cut(s) 63, 105
BstFNI CGCG 1 cut(s) 302
BstH2I RGCGCY 1 cut(s) 126
BstHHI GCGC 1 cut(s) 125
BstKTI GATC 4 cut(s) 104, 161, 336, 567
BstMBI GATC 4 cut(s) 101, 158, 333, 564
BstMCI CGRYCG 2 cut(s) 309, 558
BstNI CCWGG 2 cut(s) 145, 407
BstSCI CCNGG 4 cut(s) 143, 286, 405, 557
BstSLI GKGCMC 1 cut(s) 573
BstUI CGCG 1 cut(s) 302
BstV1I GCAGC 6 cut(s) 25, 31, 37, 43, 399, 468
BstX2I RGATCY 1 cut(s) 158
BstYI RGATCY 1 cut(s) 158
BstZI CGGCCG 2 cut(s) 306, 555
BsuI GTATCC 1 cut(s) 508
BsuRI GGCC 7 cut(s) 148, 308, 372, 405, 468, 557, 571
BtsCI GGATG 2 cut(s) 63, 105
BtsI GCAGTG 2 cut(s) 45, 448
BtsIMutI CAGTG 2 cut(s) 45, 448
BtuMI TCGCGA 1 cut(s) 302
Cac8I GCNNGC 1 cut(s) 293
CfoI GCGC 1 cut(s) 125
Cfr13I GGNCC 8 cut(s) 147, 352, 370, 390, 403, 466, 569, 570
DdeI CTNAG 1 cut(s) 167
DinI GGCGCC 1 cut(s) 124
DpnI GATC 4 cut(s) 103, 160, 335, 566
DpnII GATC 4 cut(s) 101, 158, 333, 564
EaeI YGGCCR 2 cut(s) 306, 555
EagI CGGCCG 2 cut(s) 306, 555
Eam1104I CTCTTC 1 cut(s) 193
EarI CTCTTC 1 cut(s) 193
EciI GGCGGA 1 cut(s) 181
EclXI CGGCCG 2 cut(s) 306, 555
Eco130I CCWWGG 1 cut(s) 469
Eco24I GRGCYC 1 cut(s) 573
Eco47I GGWCC 2 cut(s) 352, 390
Eco52I CGGCCG 2 cut(s) 306, 555
Eco57I CTGAAG 2 cut(s) 328, 540
Eco88I CYCGRG 1 cut(s) 149
EcoO109I RGGNCCY 1 cut(s) 352
EcoRII CCWGG 2 cut(s) 143, 405
EcoT14I CCWWGG 1 cut(s) 469
EcoT38I GRGCYC 1 cut(s) 573
EgeI GGCGCC 1 cut(s) 124
EheI GGCGCC 1 cut(s) 124
ErhI CCWWGG 1 cut(s) 469
FaiI YATR 1 cut(s) 579
FauI CCCGC 3 cut(s) 88, 140, 227
FblI GTMKAC 1 cut(s) 172
FokI GGATG 2 cut(s) 50, 92
FriOI GRGCYC 1 cut(s) 573
GlaI GCGC 1 cut(s) 124
HaeII RGCGCY 1 cut(s) 126
HaeIII GGCC 7 cut(s) 148, 308, 372, 405, 468, 557, 571
HapII CCGG 2 cut(s) 288, 558
HhaI GCGC 1 cut(s) 125
Hin1I GRCGYC 2 cut(s) 90, 123
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HincII GTYRAC 1 cut(s) 173
HindII GTYRAC 1 cut(s) 173
HinfI GANTC 2 cut(s) 179, 204
HpaII CCGG 2 cut(s) 288, 558
HphI GGTGA 2 cut(s) 64, 343
Hpy166II GTNNAC 1 cut(s) 173
Hpy188I TCNGA 3 cut(s) 158, 281, 590
Hpy188III TCNNGA 4 cut(s) 99, 109, 301, 512
Hpy8I GTNNAC 1 cut(s) 173
Hpy99I CGWCG 3 cut(s) 307, 317, 320
HpyAV CCTTC 2 cut(s) 125, 515
HpyCH4III ACNGT 1 cut(s) 586
HpyCH4IV ACGT 1 cut(s) 90
HpyF3I CTNAG 1 cut(s) 167
HpySE526I ACGT 1 cut(s) 90
Hsp92I GRCGYC 2 cut(s) 90, 123
HspAI GCGC 1 cut(s) 123
KasI GGCGCC 1 cut(s) 122
Kzo9I GATC 4 cut(s) 101, 158, 333, 564
Lsp1109I GCAGC 6 cut(s) 25, 31, 37, 43, 399, 468
LweI GCATC 1 cut(s) 72
MaeII ACGT 1 cut(s) 90
MaeIII GTNAC 1 cut(s) 70
MalI GATC 4 cut(s) 103, 160, 335, 566
MboI GATC 4 cut(s) 101, 158, 333, 564
MboII GAAGA 2 cut(s) 180, 334
MflI RGATCY 1 cut(s) 158
MhlI GDGCHC 1 cut(s) 573
MluCI AATT 1 cut(s) 474
Mly113I GGCGCC 1 cut(s) 123
MlyI GAGTC 2 cut(s) 173, 198
MnlI CCTC 6 cut(s) 172, 257, 278, 284, 343, 562
MslI CAYNNNNRTG 1 cut(s) 381
MspA1I CMGCKG 1 cut(s) 86
MspI CCGG 2 cut(s) 288, 558
MspR9I CCNGG 4 cut(s) 145, 288, 407, 559
MvaI CCWGG 2 cut(s) 145, 407
MvnI CGCG 1 cut(s) 302
NarI GGCGCC 1 cut(s) 123
NciI CCSGG 2 cut(s) 288, 559
NdeII GATC 4 cut(s) 101, 158, 333, 564
NlaIV GGNNCC 4 cut(s) 124, 160, 354, 571
NmeAIII GCCGAG 1 cut(s) 533
NmuCI GTSAC 1 cut(s) 70
NruI TCGCGA 1 cut(s) 302
OliI CACNNNNGTG 1 cut(s) 381
PleI GAGTC 2 cut(s) 173, 198
PluTI GGCGCC 1 cut(s) 126
PpsI GAGTC 2 cut(s) 173, 198
PpuMI RGGWCCY 1 cut(s) 352
Psp5II RGGWCCY 1 cut(s) 352
Psp6I CCWGG 2 cut(s) 143, 405
PspGI CCWGG 2 cut(s) 143, 405
PspN4I GGNNCC 4 cut(s) 124, 160, 354, 571
PspOMI GGGCCC 1 cut(s) 569
PspPI GGNCC 8 cut(s) 147, 352, 370, 390, 403, 466, 569, 570
PspPPI RGGWCCY 1 cut(s) 352
PsuI RGATCY 1 cut(s) 158
RruI TCGCGA 1 cut(s) 302
RseI CAYNNNNRTG 1 cut(s) 381
SalI GTCGAC 1 cut(s) 171
Sau3AI GATC 4 cut(s) 101, 158, 333, 564
Sau96I GGNCC 8 cut(s) 147, 352, 370, 390, 403, 466, 569, 570
SchI GAGTC 2 cut(s) 173, 198
ScrFI CCNGG 4 cut(s) 145, 288, 407, 559
SduI GDGCHC 1 cut(s) 573
SetI ASST 4 cut(s) 56, 93, 117, 578
SfaNI GCATC 1 cut(s) 72
SfoI GGCGCC 1 cut(s) 124
SinI GGWCC 2 cut(s) 352, 390
SmiMI CAYNNNNRTG 1 cut(s) 381
Sse9I AATT 1 cut(s) 474
SspDI GGCGCC 1 cut(s) 122
StyD4I CCNGG 4 cut(s) 143, 286, 405, 557
StyI CCWWGG 1 cut(s) 469
TaaI ACNGT 1 cut(s) 586
TaiI ACGT 1 cut(s) 93
TaqI TCGA 1 cut(s) 172
TasI AATT 1 cut(s) 474
TauI GCSGC 9 cut(s) 19, 25, 31, 37, 86, 264, 311, 375, 551
TscAI CASTG 2 cut(s) 52, 448
TseFI GTSAC 1 cut(s) 70
TseI GCWGC 6 cut(s) 13, 19, 25, 31, 412, 481
Tsp45I GTSAC 1 cut(s) 70
TspDTI ATGAA 1 cut(s) 558
TspGWI ACGGA 1 cut(s) 382
TspRI CASTG 2 cut(s) 52, 448
VpaK11BI GGWCC 2 cut(s) 352, 390
XcmI CCANNNNNNNNNTGG 1 cut(s) 116
XmiI GTMKAC 1 cut(s) 172
ZraI GACGTC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.