MD11G1046400.v1.1

PRA1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0016200
Physical Location & Seq
Reverse (-)
4029448 .. 4030017
570 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1046400.v1.1.491

Sequence Viewer

Length: 570 bp
ATGTCGCCGTCGCAGTCCGTCACAGCAACAACCTATACCAGCATACCCATTTCGGTCTCGGGCTCCGACGCCATCACCCGATCCATCAAAAACCTCAACGACGCCGTTTCCCGCCGCCGTCCATGGTCCGAATTCGCCTCCTCGATCTCCCGACCCGACTCCTTATCCTCTGCCCTGACCAGGATCCAGACCAACGCCAACCACTTCCGGGTCAACTACTTGTTATTGATCGGATCCTGCGGGGTGCTATCCCTCATCGGGAGCCCGTGCTGGCTTCTGGTGACGGTGGCCGTCGTGGGGTTGTGGCTGGTGATCTATGTTTTTAGGGAGGACCCACTTGAGGTGTGGGGCCACCACGTCAGTAATTGGGCCGTGATGGCGGGCCTGGTGGTTGTTTCTGTTTTGGTTGCTTGGGCCAGCGGTGGGCTTGGGAGTATTGCATTGGGCCTTGGAGTTGGGCTTATGTTGTGTGCGGTGCACGGGCTGCTTACGAACACAGAAGGCTTGTTTCTCAACGAGACCGAAGCTGCTTCTCAGGGATTGATTGGGCCCACATCTTCATCAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006810 GO:0008150 GO:0012505 GO:0016192 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PRA1

Protein Analysis

190

Amino Acids

19.91

Weight (kDa)

6.39

Isoelectric Point (pI)

41.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 35 - 173 1.1e-35 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016200)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G56230
fragaria_vesca FvH4_3g41570
malus_domestica MD03G1043200.v1.1 MD03G1043300.v1.1 MD11G1046400.v1.1
prunus_persica Prupe.6G035700_v2.0.a1
pyrus_communis pycom03g03350 pycom11g03780
rosa_chinensis RchiOBHm_Chr5g0074561
rosa_laevigata RLG00000036487
rosa_roxburghii Rroxscaffold_1G00007000
rosa_rugosa Rorug05G0432700
rosa_samantha Rh5BG510700 Rh5CG534500
rosa_wichuraiana Rw5G045410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 112, 115, 240, 380, 420, 473
AclWI GGATC 5 cut(s) 75, 178, 191, 228, 241
AcoI YGGCCR 1 cut(s) 288
AcsI RAATTY 1 cut(s) 131
AcyI GRCGYC 2 cut(s) 69, 102
AfiI CCNNNNNNNGG 6 cut(s) 180, 208, 258, 297, 340, 423
AjiI CACGTC 1 cut(s) 358
AjnI CCWGG 2 cut(s) 179, 384
AluBI AGCT 1 cut(s) 527
AluI AGCT 1 cut(s) 527
Alw21I GWGCWC 1 cut(s) 480
Alw26I GTCTC 2 cut(s) 61, 512
Alw44I GTGCAC 1 cut(s) 476
AlwI GGATC 5 cut(s) 75, 178, 191, 228, 241
Ama87I CYCGRG 1 cut(s) 58
AoxI GGCC 7 cut(s) 288, 349, 369, 382, 414, 445, 548
ApaI GGGCCC 1 cut(s) 552
ApaLI GTGCAC 1 cut(s) 476
ApeKI GCWGC 2 cut(s) 484, 527
ApoI RAATTY 1 cut(s) 131
AspS9I GGNCC 9 cut(s) 126, 331, 349, 369, 382, 414, 445, 548, 549
AsuC2I CCSGG 1 cut(s) 209
AsuHPI GGTGA 3 cut(s) 67, 292, 322
AvaI CYCGRG 1 cut(s) 58
AvaII GGWCC 2 cut(s) 126, 331
BaeGI GKGCMC 2 cut(s) 480, 552
BamHI GGATCC 2 cut(s) 183, 233
BanII GRGCYC 3 cut(s) 65, 266, 552
Bbv12I GWGCWC 1 cut(s) 480
BbvI GCAGC 2 cut(s) 471, 514
BccI CCATC 3 cut(s) 80, 92, 370
BceAI ACGGC 4 cut(s) 89, 102, 275, 356
BciT130I CCWGG 2 cut(s) 181, 386
BcnI CCSGG 1 cut(s) 209
BcoDI GTCTC 2 cut(s) 61, 512
BglI GCCNNNNNGGC 1 cut(s) 377
BisI GCNGC 3 cut(s) 115, 485, 528
BlsI GCNGC 3 cut(s) 116, 486, 529
Bme1390I CCNGG 3 cut(s) 181, 209, 386
Bme18I GGWCC 2 cut(s) 126, 331
BmeT110I CYCGRG 1 cut(s) 58
BmgBI CACGTC 1 cut(s) 358
BmgT120I GGNCC 9 cut(s) 126, 331, 349, 369, 382, 414, 445, 548, 549
BmiI GGNNCC 7 cut(s) 64, 185, 235, 263, 333, 350, 550
BmrFI CCNGG 3 cut(s) 181, 209, 386
BpuEI CTTGAG 1 cut(s) 359
BpuMI CCSGG 1 cut(s) 209
BsaHI GRCGYC 2 cut(s) 69, 102
BsaI GGTCTC 2 cut(s) 61, 512
BsaJI CCNNGG 2 cut(s) 122, 448
Bsc4I CCNNNNNNNGG 6 cut(s) 180, 208, 258, 297, 340, 423
BseBI CCWGG 2 cut(s) 181, 386
BseDI CCNNGG 2 cut(s) 122, 448
BseLI CCNNNNNNNGG 6 cut(s) 180, 208, 258, 297, 340, 423
BseMII CTCAG 1 cut(s) 548
BseRI GAGGAG 1 cut(s) 130
BseSI GKGCMC 2 cut(s) 480, 552
BseXI GCAGC 2 cut(s) 471, 514
BshFI GGCC 7 cut(s) 290, 351, 371, 384, 416, 447, 550
BsiHKAI GWGCWC 1 cut(s) 480
BsiHKCI CYCGRG 1 cut(s) 58
BsiSI CCGG 1 cut(s) 208
BslI CCNNNNNNNGG 6 cut(s) 180, 208, 258, 297, 340, 423
BsmAI GTCTC 2 cut(s) 61, 512
BsnI GGCC 7 cut(s) 290, 351, 371, 384, 416, 447, 550
Bso31I GGTCTC 2 cut(s) 61, 512
BsoBI CYCGRG 1 cut(s) 58
Bsp120I GGGCCC 1 cut(s) 548
Bsp1286I GDGCHC 4 cut(s) 65, 266, 480, 552
Bsp143I GATC 6 cut(s) 80, 144, 183, 228, 233, 312
Bsp19I CCATGG 1 cut(s) 122
BspACI CCGC 6 cut(s) 112, 115, 240, 380, 420, 473
BspANI GGCC 7 cut(s) 290, 351, 371, 384, 416, 447, 550
BspCNI CTCAG 1 cut(s) 547
BspLI GGNNCC 7 cut(s) 64, 185, 235, 263, 333, 350, 550
BspPI GGATC 5 cut(s) 75, 178, 191, 228, 241
BspTNI GGTCTC 2 cut(s) 61, 512
BssECI CCNNGG 2 cut(s) 122, 448
BssMI GATC 6 cut(s) 80, 144, 183, 228, 233, 312
BssNI GRCGYC 2 cut(s) 69, 102
BssT1I CCWWGG 2 cut(s) 122, 448
Bst2UI CCWGG 2 cut(s) 181, 386
Bst4CI ACNGT 1 cut(s) 286
BstACI GRCGYC 2 cut(s) 69, 102
BstAPI GCANNNNNTGC 1 cut(s) 484
BstC8I GCNNGC 3 cut(s) 272, 382, 418
BstDEI CTNAG 1 cut(s) 534
BstDSI CCRYGG 1 cut(s) 122
BstKTI GATC 6 cut(s) 83, 147, 186, 231, 236, 315
BstMAI GTCTC 2 cut(s) 61, 512
BstMBI GATC 6 cut(s) 80, 144, 183, 228, 233, 312
BstMWI GCNNNNNNNGC 2 cut(s) 377, 484
BstNI CCWGG 2 cut(s) 181, 386
BstSCI CCNGG 3 cut(s) 179, 207, 384
BstSLI GKGCMC 2 cut(s) 480, 552
BstV1I GCAGC 2 cut(s) 471, 514
BstX2I RGATCY 2 cut(s) 183, 233
BstYI RGATCY 2 cut(s) 183, 233
BsuRI GGCC 7 cut(s) 290, 351, 371, 384, 416, 447, 550
BtgI CCRYGG 1 cut(s) 122
BtrI CACGTC 1 cut(s) 358
Cac8I GCNNGC 3 cut(s) 272, 382, 418
Cfr13I GGNCC 9 cut(s) 126, 331, 349, 369, 382, 414, 445, 548, 549
CseI GACGC 2 cut(s) 77, 110
CviAII CATG 1 cut(s) 123
DdeI CTNAG 1 cut(s) 534
DpnI GATC 6 cut(s) 82, 146, 185, 230, 235, 314
DpnII GATC 6 cut(s) 80, 144, 183, 228, 233, 312
EaeI YGGCCR 1 cut(s) 288
Eco130I CCWWGG 2 cut(s) 122, 448
Eco24I GRGCYC 3 cut(s) 65, 266, 552
Eco31I GGTCTC 2 cut(s) 61, 512
Eco47I GGWCC 2 cut(s) 126, 331
Eco88I CYCGRG 1 cut(s) 58
EcoO109I RGGNCCY 1 cut(s) 331
EcoRI GAATTC 1 cut(s) 131
EcoRII CCWGG 2 cut(s) 179, 384
EcoT14I CCWWGG 2 cut(s) 122, 448
EcoT38I GRGCYC 3 cut(s) 65, 266, 552
ErhI CCWWGG 2 cut(s) 122, 448
FaeI CATG 1 cut(s) 126
FaiI YATR 5 cut(s) 36, 44, 124, 318, 464
FatI CATG 1 cut(s) 122
FauI CCCGC 3 cut(s) 119, 233, 373
Fnu4HI GCNGC 3 cut(s) 115, 485, 528
FriOI GRGCYC 3 cut(s) 65, 266, 552
Fsp4HI GCNGC 3 cut(s) 115, 485, 528
GluI GCNGC 3 cut(s) 115, 485, 528
HaeIII GGCC 7 cut(s) 290, 351, 371, 384, 416, 447, 550
HapII CCGG 1 cut(s) 208
HgaI GACGC 2 cut(s) 77, 110
Hin1I GRCGYC 2 cut(s) 69, 102
Hin1II CATG 1 cut(s) 126
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HinfI GANTC 1 cut(s) 158
HpaII CCGG 1 cut(s) 208
HphI GGTGA 3 cut(s) 67, 292, 322
Hpy166II GTNNAC 2 cut(s) 214, 478
Hpy188I TCNGA 3 cut(s) 67, 130, 233
Hpy188III TCNNGA 3 cut(s) 150, 187, 259
Hpy8I GTNNAC 2 cut(s) 214, 478
Hpy99I CGWCG 4 cut(s) 13, 71, 104, 296
HpyAV CCTTC 1 cut(s) 494
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4IV ACGT 1 cut(s) 357
HpyCH4V TGCA 2 cut(s) 440, 478
HpyF10VI GCNNNNNNNGC 2 cut(s) 377, 484
HpyF3I CTNAG 1 cut(s) 534
HpySE526I ACGT 1 cut(s) 357
Hsp92I GRCGYC 2 cut(s) 69, 102
Hsp92II CATG 1 cut(s) 126
Kzo9I GATC 6 cut(s) 80, 144, 183, 228, 233, 312
LmnI GCTCC 2 cut(s) 68, 261
Lsp1109I GCAGC 2 cut(s) 471, 514
MaeII ACGT 1 cut(s) 357
MaeIII GTNAC 2 cut(s) 19, 280
MalI GATC 6 cut(s) 82, 146, 185, 230, 235, 314
MboI GATC 6 cut(s) 80, 144, 183, 228, 233, 312
MboII GAAGA 1 cut(s) 549
MflI RGATCY 2 cut(s) 183, 233
MhlI GDGCHC 4 cut(s) 65, 266, 480, 552
MluCI AATT 2 cut(s) 131, 364
MlyI GAGTC 1 cut(s) 152
MmeI TCCRAC 1 cut(s) 90
MnlI CCTC 7 cut(s) 104, 148, 151, 178, 263, 322, 334
MspA1I CMGCKG 1 cut(s) 420
MspI CCGG 1 cut(s) 208
MspR9I CCNGG 3 cut(s) 181, 209, 386
MvaI CCWGG 2 cut(s) 181, 386
MwoI GCNNNNNNNGC 2 cut(s) 377, 484
NciI CCSGG 1 cut(s) 209
NcoI CCATGG 1 cut(s) 122
NdeII GATC 6 cut(s) 80, 144, 183, 228, 233, 312
NlaIII CATG 1 cut(s) 126
NlaIV GGNNCC 7 cut(s) 64, 185, 235, 263, 333, 350, 550
NmuCI GTSAC 2 cut(s) 19, 280
PkrI GCNGC 3 cut(s) 116, 486, 529
PleI GAGTC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 152
PpuMI RGGWCCY 1 cut(s) 331
Psp5II RGGWCCY 1 cut(s) 331
Psp6I CCWGG 2 cut(s) 179, 384
PspGI CCWGG 2 cut(s) 179, 384
PspN4I GGNNCC 7 cut(s) 64, 185, 235, 263, 333, 350, 550
PspOMI GGGCCC 1 cut(s) 548
PspPI GGNCC 9 cut(s) 126, 331, 349, 369, 382, 414, 445, 548, 549
PspPPI RGGWCCY 1 cut(s) 331
PsuI RGATCY 2 cut(s) 183, 233
SatI GCNGC 3 cut(s) 115, 485, 528
Sau3AI GATC 6 cut(s) 80, 144, 183, 228, 233, 312
Sau96I GGNCC 9 cut(s) 126, 331, 349, 369, 382, 414, 445, 548, 549
SchI GAGTC 1 cut(s) 152
ScrFI CCNGG 3 cut(s) 181, 209, 386
SduI GDGCHC 4 cut(s) 65, 266, 480, 552
SetI ASST 5 cut(s) 35, 96, 345, 360, 529
SinI GGWCC 2 cut(s) 126, 331
SmlI CTYRAG 1 cut(s) 338
SmoI CTYRAG 1 cut(s) 338
Sse9I AATT 2 cut(s) 131, 364
SsiI CCGC 6 cut(s) 112, 115, 240, 380, 420, 473
StyD4I CCNGG 3 cut(s) 179, 207, 384
StyI CCWWGG 2 cut(s) 122, 448
TaaI ACNGT 1 cut(s) 286
TaiI ACGT 1 cut(s) 360
TaqI TCGA 1 cut(s) 143
TaqII GACCGA 2 cut(s) 43, 536
TasI AATT 2 cut(s) 131, 364
TauI GCSGC 1 cut(s) 117
TseFI GTSAC 2 cut(s) 19, 280
TseI GCWGC 2 cut(s) 484, 527
Tsp45I GTSAC 2 cut(s) 19, 280
TspDTI ATGAA 1 cut(s) 549
TspGWI ACGGA 1 cut(s) 7
VneI GTGCAC 1 cut(s) 476
VpaK11BI GGWCC 2 cut(s) 126, 331
XapI RAATTY 1 cut(s) 131
XcmI CCANNNNNNNNNTGG 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.