Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0011549

Physical Location & Seq

Genomic Coordinates Reverse (-)

4031835 .. 4037497

Gene Structure 5663 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1050800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1722 bp

ATGTCGTATGTGCCTCCGCACCTGAGGTCGAGCTCAAGAACCACCACGACAATCACCACCACCACCACTGCCGAAGCCTCGTCTCGAACCCTAGACGATCAGAGCAAGCTCTCCTACTCCTCCTCCAACTCTCACTTCAACTACGACAAGTCGTCGTCCGGTGGTGCTTCCTTCTCCAGCTTCTCTCACTCCAATGCCTCCTCTCGCTGGAGCTCCGGCGCTCTCTCCCAGGCCAGCAGAGCTCGCCCGGATTCCGTCGTCCCTCAGTGGAAGCCCTCCGACCGCGTCCTCCGCATGAATCCTGAAATGATTGAAGAGGTTTGTTTGCGGCTTAATTTGGATGTTTCTGTTAGTTCCGACTCTGCTCCTGCTCCGGCACCGATCGAGTCTTTTACAGATATGTGTTTGCACCCTAGCATCATGAAGGATATCGACAATCACGGATACACCACACCGACATCCATTCAGGCTCAAGCTATGCCGGTTGCTCTCACTGGGAGGGACTTATTAGGTTGTGCTGAAACTGGCAGTGGAAAAACTGCTGCATTCGCCATTCCCATGATACAGCATTGCTTGGCTCAACCTCCTGTTCAGCGCGGTGATGGACCGCTAGCTCTGGTATTGGCTCCGACTAGAGAGCTTGCCCAACAAATTGAAAAAGAGGTCAAAGCGTTTAGTGCATCTCTCGAGTCCTTCAGAACTGCAATTGTGGTGGGTGGAACCAACATATCTGAGCAGAGGTCTGAGCTACGAGCAGGAGTTGATGTAGTGGTTGCTACTCCTGGAAGATTTATAGATCATTTACAACAAGGGAACACTTCTATCACTAGAATTTCGTTTGTTGTTTTAGATGAAGCTGATAGAATGCTTGACATGGGATTTGAACCACAGATAAGAGAGGTGATGCGGAACCTTTCTGAAAAGCATCAAACTTTGCTGTTTAGTGCAACTATGCCTGAAGAGATCGAAGAATTAGCTCAGGAATACTTGACTAAACCAATGCAAGTGAAGGTGGGAAAAGTGAGTAGCCCTACAGCAAATGTGACTCAGACTTTGGAGAAGGTTCCTGAGAGTGAGAAGACTGATCAGCTATTGGCTTTGCTCGTAGAAGAGGCATCTCGGGTTGAAGGAACTGGCCATCCCTTTCCCTTAACGATTGTGTTTGTTGAAAGGAAGACGAGATGTGACGAAGTTGCTGAAGCTTTGGTAGCACAAGGGTTGCATGCAGTTGCTCTTCATGGTGGTCGTACACAAACTGAAAGAGAGGCTGCTCTACGTGATTTTAGAAAAGGCACTACCAGTATTTTGGTTGCCACTGATGTTGCGTCTCGCGGTTTGGATGTCTCAGGAGTTGCACATGTTATCAATTTGGATCTTCCAAAGAGCATGGAAGATTATGTACACCGGATTGGAAGGACGGGAAGAGCAGGATCAATGGGACAATCTACGTCCTTTTACACAGATCGTGATATGTTCCTCGTGGCAAATATAAAGAAAGCGATATCAGATGCTGGATCTGGGAATGCTGTGGCTTTCGCAACTGGGAAGACTGCAAGAAGGAAAGAAAAAGAAGCAGCAGCTGCTGAAAAGCAAGCTAGGGTTGCTTCCAAAAGCTCAATAGCAGGACCAACATCAGTAAACATTGAGGACAAGTATCGGTTCATGTTTGCGGATTCGAACAATAAAAAAGAAGGGGCAGCAGATAGTGCTTGGGACGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko03040 ko05202 ko05205 map03040 map05202 map05205

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

574

Amino Acids

61.94

Weight (kDa)

5.97

Isoelectric Point (pI)

45.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	153 - 325	3.5e-52	DEAD/DEAH box helicase
ResIII	PF04851	173 - 319	3.4e-08	Type III restriction enzyme, res subunit
Helicase_C	PF00271	380 - 477	8.4e-30	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011549)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g40600 FvH4_3g40620
malus_domestica	MD03G1050800.v1.1 MD11G1052800.v1.1
prunus_persica	Prupe.6G040300_v2.0.a1 Prupe.6G040300_v2.0.a1
pyrus_communis	pycom03g04010 pycom11g04300
rosa_chinensis	RchiOBHm_Chr5g0073371
rosa_laevigata	RLG00000036381
rosa_multiflora	Rmu_sc0024948.1_g000001
rosa_roxburghii	Rroxscaffold_1G00007870
rosa_rugosa	Rorug05G0423000 Rorug05G0423100 Rorug05G0423200
rosa_samantha	Rh5AG479600 Rh5BG500800 Rh5CG523900 Rh5DG512700
rosa_wichuraiana	Rw5G044590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	376
AccII	CGCG	3 cut(s)	285, 597, 1332
AciI	CCGC	9 cut(s)	17, 283, 292, 328, 597, 608, 907, 1332, 1670
AclWI	GGATC	3 cut(s)	1380, 1438, 1522
AcoI	YGGCCR	1 cut(s)	1135
AcsI	RAATTY	1 cut(s)	831
AcuI	CTGAAG	3 cut(s)	679, 978, 1218
AfaI	GTAC	2 cut(s)	1249, 1401
AfiI	CCNNNNNNNGG	1 cut(s)	207
AflIII	ACRYGT	1 cut(s)	1357
AgsI	TTSAA	6 cut(s)	139, 314, 656, 884, 1127, 1169
AhdI	GACNNNNNGTC	1 cut(s)	151
AjnI	CCWGG	2 cut(s)	228, 781
AjuI	GAANNNNNNNTTGG	2 cut(s)	119, 151
AloI	GAACNNNNNNTCC	2 cut(s)	1123, 1155
Alw21I	GWGCWC	3 cut(s)	35, 215, 244
Alw26I	GTCTC	3 cut(s)	87, 1332, 1348
AlwI	GGATC	3 cut(s)	1380, 1438, 1522
AlwNI	CAGNNNCTG	3 cut(s)	1511, 1580, 1583
Ama87I	CYCGRG	2 cut(s)	686, 1119
AoxI	GGCC	2 cut(s)	231, 1135
ApeKI	GCWGC	6 cut(s)	542, 1268, 1574, 1577, 1580, 1697
ApoI	RAATTY	1 cut(s)	831
ArsI	GACNNNNNNTTYG	4 cut(s)	863, 895, 1036, 1068
AspLEI	GCGC	2 cut(s)	221, 597
AspS9I	GGNCC	2 cut(s)	605, 1625
AsuC2I	CCSGG	1 cut(s)	248
AsuHPI	GGTGA	3 cut(s)	46, 611, 913
AsuII	TTCGAA	1 cut(s)	1676
AsuNHI	GCTAGC	1 cut(s)	610
AvaI	CYCGRG	2 cut(s)	686, 1119
AvaII	GGWCC	2 cut(s)	605, 1625
AxyI	CCTNAGG	1 cut(s)	23
BalI	TGGCCA	1 cut(s)	1137
BanI	GGYRCC	1 cut(s)	376
BanII	GRGCYC	3 cut(s)	35, 215, 244
BarI	GAAGNNNNNNTAC	2 cut(s)	1383, 1415
BauI	CACGAG	1 cut(s)	1478
BbsI	GAAGAC	3 cut(s)	1085, 1181, 1553
Bbv12I	GWGCWC	3 cut(s)	35, 215, 244
BbvI	GCAGC	6 cut(s)	529, 1255, 1567, 1586, 1589, 1709
BccI	CCATC	2 cut(s)	596, 1146
BciT130I	CCWGG	2 cut(s)	230, 783
BciVI	GTATCC	1 cut(s)	437
BclI	TGATCA	1 cut(s)	1084
BcnI	CCSGG	1 cut(s)	248
BcoDI	GTCTC	3 cut(s)	87, 1332, 1348
BfaI	CTAG	6 cut(s)	92, 414, 611, 633, 828, 1596
BfmI	CTRYAG	1 cut(s)	1032
BfoI	RGCGCY	1 cut(s)	222
BfuI	GTATCC	1 cut(s)	437
BisI	GCNGC	7 cut(s)	329, 543, 1269, 1575, 1578, 1581, 1698
BlsI	GCNGC	7 cut(s)	330, 544, 1270, 1576, 1579, 1582, 1699
Bme1390I	CCNGG	3 cut(s)	230, 248, 783
Bme18I	GGWCC	2 cut(s)	605, 1625
BmeRI	GACNNNNNGTC	1 cut(s)	151
BmeT110I	CYCGRG	2 cut(s)	686, 1119
BmgT120I	GGNCC	2 cut(s)	605, 1625
BmiI	GGNNCC	5 cut(s)	378, 627, 721, 911, 1065
BmrFI	CCNGG	3 cut(s)	230, 248, 783
BmrI	ACTGGG	2 cut(s)	504, 1551
BmsI	GCATC	6 cut(s)	426, 689, 894, 934, 1124, 1498
BmtI	GCTAGC	1 cut(s)	614
BmuI	ACTGGG	2 cut(s)	504, 1551
BpiI	GAAGAC	3 cut(s)	1085, 1181, 1553
BpmI	CTGGAG	2 cut(s)	160, 229
Bpu10I	CCTNAGC	1 cut(s)	978
Bpu14I	TTCGAA	1 cut(s)	1676
BpuEI	CTTGAG	2 cut(s)	19, 456
BpuMI	CCSGG	1 cut(s)	248
BsaAI	YACGTR	1 cut(s)	1277
BsaJI	CCNNGG	1 cut(s)	228
BsaWI	WCCGGW	2 cut(s)	158, 1404
BsaXI	ACNNNNNCTCC	4 cut(s)	107, 137, 750, 780
Bsc4I	CCNNNNNNNGG	1 cut(s)	207
Bse118I	RCCGGY	1 cut(s)	481
Bse1I	ACTGG	5 cut(s)	499, 529, 1138, 1299, 1546
Bse21I	CCTNAGG	1 cut(s)	23
Bse3DI	GCAATG	1 cut(s)	568
BseBI	CCWGG	2 cut(s)	230, 783
BseDI	CCNNGG	1 cut(s)	228
BseGI	GGATG	4 cut(s)	346, 458, 1138, 1345
BseLI	CCNNNNNNNGG	1 cut(s)	207
BseMI	GCAATG	1 cut(s)	568
BseMII	CTCAG	8 cut(s)	14, 278, 723, 735, 992, 1059, 1061, 1359
BseNI	ACTGG	5 cut(s)	499, 529, 1138, 1299, 1546
BseRI	GAGGAG	3 cut(s)	109, 112, 190
BseXI	GCAGC	6 cut(s)	529, 1255, 1567, 1586, 1589, 1709
Bsh1236I	CGCG	3 cut(s)	285, 597, 1332
Bsh1285I	CGRYCG	2 cut(s)	283, 384
BshFI	GGCC	2 cut(s)	233, 1137
BshNI	GGYRCC	1 cut(s)	376
BsiEI	CGRYCG	2 cut(s)	283, 384
BsiHKAI	GWGCWC	3 cut(s)	35, 215, 244
BsiHKCI	CYCGRG	2 cut(s)	686, 1119
BsiSI	CCGG	6 cut(s)	159, 216, 248, 374, 482, 1405
BslFI	GGGAC	3 cut(s)	245, 515, 1452
BslI	CCNNNNNNNGG	1 cut(s)	207
BsmAI	GTCTC	3 cut(s)	87, 1332, 1348
BsmBI	CGTCTC	2 cut(s)	87, 1332
BsmFI	GGGAC	3 cut(s)	245, 515, 1452
BsmI	GAATGC	3 cut(s)	545, 870, 1528
BsnI	GGCC	2 cut(s)	233, 1137
BsoBI	CYCGRG	2 cut(s)	686, 1119
Bsp119I	TTCGAA	1 cut(s)	1676
Bsp1286I	GDGCHC	3 cut(s)	35, 215, 244
Bsp1407I	TGTACA	1 cut(s)	1399
Bsp143I	GATC	9 cut(s)	97, 381, 796, 963, 1084, 1372, 1430, 1462, 1514
BspACI	CCGC	9 cut(s)	17, 283, 292, 328, 597, 608, 907, 1332, 1670
BspANI	GGCC	2 cut(s)	233, 1137
BspCNI	CTCAG	8 cut(s)	15, 277, 724, 736, 991, 1060, 1060, 1358
BspFNI	CGCG	3 cut(s)	285, 597, 1332
BspHI	TCATGA	1 cut(s)	420
BspLI	GGNNCC	5 cut(s)	378, 627, 721, 911, 1065
BspOI	GCTAGC	1 cut(s)	614
BspPI	GGATC	3 cut(s)	1380, 1438, 1522
BspQI	GCTCTTC	2 cut(s)	1239, 1417
BspT104I	TTCGAA	1 cut(s)	1676
BspT107I	GGYRCC	1 cut(s)	376
BsrDI	GCAATG	1 cut(s)	568
BsrFI	RCCGGY	1 cut(s)	481
BsrGI	TGTACA	1 cut(s)	1399
BsrI	ACTGG	5 cut(s)	499, 529, 1138, 1299, 1546
BssAI	RCCGGY	1 cut(s)	481
BssECI	CCNNGG	1 cut(s)	228
BssMI	GATC	9 cut(s)	97, 381, 796, 963, 1084, 1372, 1430, 1462, 1514
BssSI	CACGAG	1 cut(s)	1478
Bst2BI	CACGAG	1 cut(s)	1478
Bst2UI	CCWGG	2 cut(s)	230, 783
Bst6I	CTCTTC	5 cut(s)	309, 954, 1104, 1239, 1417
BstAPI	GCANNNNNTGC	2 cut(s)	1580, 1706
BstAUI	TGTACA	1 cut(s)	1399
BstBAI	YACGTR	1 cut(s)	1277
BstBI	TTCGAA	1 cut(s)	1676
BstC8I	GCNNGC	7 cut(s)	107, 235, 244, 612, 642, 1224, 1593
BstDEI	CTNAG	8 cut(s)	23, 264, 732, 744, 978, 1047, 1068, 1345
BstF5I	GGATG	4 cut(s)	346, 458, 1138, 1345
BstFNI	CGCG	3 cut(s)	285, 597, 1332
BstH2I	RGCGCY	1 cut(s)	222
BstHHI	GCGC	2 cut(s)	221, 597
BstKTI	GATC	9 cut(s)	100, 384, 799, 966, 1087, 1375, 1433, 1465, 1517
BstMAI	GTCTC	3 cut(s)	87, 1332, 1348
BstMBI	GATC	9 cut(s)	97, 381, 796, 963, 1084, 1372, 1430, 1462, 1514
BstMCI	CGRYCG	2 cut(s)	283, 384
BstMWI	GCNNNNNNNGC	9 cut(s)	239, 243, 291, 548, 677, 1208, 1580, 1601, 1706
BstNI	CCWGG	2 cut(s)	230, 783
BstNSI	RCATGY	2 cut(s)	1226, 1361
BstSCI	CCNGG	3 cut(s)	228, 246, 781
BstSFI	CTRYAG	1 cut(s)	1032
BstUI	CGCG	3 cut(s)	285, 597, 1332
BstV1I	GCAGC	6 cut(s)	529, 1255, 1567, 1586, 1589, 1709
BstV2I	GAAGAC	3 cut(s)	1085, 1181, 1553
BstX2I	RGATCY	2 cut(s)	1372, 1514
BstXI	CCANNNNNNTGG	1 cut(s)	1306
BstYI	RGATCY	2 cut(s)	1372, 1514
Bsu36I	CCTNAGG	1 cut(s)	23
BsuI	GTATCC	1 cut(s)	437
BsuRI	GGCC	2 cut(s)	233, 1137
BtsCI	GGATG	4 cut(s)	346, 458, 1138, 1345
BtsI	GCAGTG	2 cut(s)	66, 535
BtsIMutI	CAGTG	5 cut(s)	66, 272, 492, 535, 1314
Cac8I	GCNNGC	7 cut(s)	107, 235, 244, 612, 642, 1224, 1593
CaiI	CAGNNNCTG	3 cut(s)	1511, 1580, 1583
CciI	TCATGA	1 cut(s)	420
CfoI	GCGC	2 cut(s)	221, 597
Cfr10I	RCCGGY	1 cut(s)	481
Cfr13I	GGNCC	2 cut(s)	605, 1625
CseI	GACGC	2 cut(s)	274, 1314
Csp6I	GTAC	2 cut(s)	1248, 1400
CspCI	CAANNNNNGTGG	4 cut(s)	693, 728, 1303, 1338
CviAII	CATG	9 cut(s)	295, 421, 559, 874, 1223, 1238, 1358, 1387, 1663
CviQI	GTAC	2 cut(s)	1248, 1400
DdeI	CTNAG	8 cut(s)	23, 264, 732, 744, 978, 1047, 1068, 1345
DpnI	GATC	9 cut(s)	99, 383, 798, 965, 1086, 1374, 1432, 1464, 1516
DpnII	GATC	9 cut(s)	97, 381, 796, 963, 1084, 1372, 1430, 1462, 1514
DriI	GACNNNNNGTC	1 cut(s)	151
EaeI	YGGCCR	1 cut(s)	1135
Eam1104I	CTCTTC	5 cut(s)	309, 954, 1104, 1239, 1417
Eam1105I	GACNNNNNGTC	1 cut(s)	151
EarI	CTCTTC	5 cut(s)	309, 954, 1104, 1239, 1417
Ecl136II	GAGCTC	3 cut(s)	33, 213, 242
Eco24I	GRGCYC	3 cut(s)	35, 215, 244
Eco32I	GATATC	2 cut(s)	430, 1503
Eco47I	GGWCC	2 cut(s)	605, 1625
Eco53kI	GAGCTC	3 cut(s)	33, 213, 242
Eco57I	CTGAAG	3 cut(s)	679, 978, 1218
Eco81I	CCTNAGG	1 cut(s)	23
Eco88I	CYCGRG	2 cut(s)	686, 1119
EcoICRI	GAGCTC	3 cut(s)	33, 213, 242
EcoRII	CCWGG	2 cut(s)	228, 781
EcoRV	GATATC	2 cut(s)	430, 1503
EcoT38I	GRGCYC	3 cut(s)	35, 215, 244
Esp3I	CGTCTC	2 cut(s)	87, 1332
FaeI	CATG	9 cut(s)	298, 424, 562, 877, 1226, 1241, 1361, 1390, 1666
FaqI	GGGAC	3 cut(s)	245, 515, 1452
FatI	CATG	9 cut(s)	294, 420, 558, 873, 1222, 1237, 1357, 1386, 1662
FbaI	TGATCA	1 cut(s)	1084
Fnu4HI	GCNGC	7 cut(s)	329, 543, 1269, 1575, 1578, 1581, 1698
FokI	GGATG	4 cut(s)	353, 445, 1125, 1352
FriOI	GRGCYC	3 cut(s)	35, 215, 244
Fsp4HI	GCNGC	7 cut(s)	329, 543, 1269, 1575, 1578, 1581, 1698
FspBI	CTAG	6 cut(s)	92, 414, 611, 633, 828, 1596
GlaI	GCGC	2 cut(s)	220, 596
GluI	GCNGC	7 cut(s)	329, 543, 1269, 1575, 1578, 1581, 1698
GsuI	CTGGAG	2 cut(s)	160, 229
HaeII	RGCGCY	1 cut(s)	222
HaeIII	GGCC	2 cut(s)	233, 1137
HapII	CCGG	6 cut(s)	159, 216, 248, 374, 482, 1405
HgaI	GACGC	2 cut(s)	274, 1314
HhaI	GCGC	2 cut(s)	221, 597
Hin1II	CATG	9 cut(s)	298, 424, 562, 877, 1226, 1241, 1361, 1390, 1666
Hin6I	GCGC	2 cut(s)	219, 595
HinP1I	GCGC	2 cut(s)	219, 595
HindIII	AAGCTT	1 cut(s)	1200
HinfI	GANTC	7 cut(s)	251, 298, 359, 386, 689, 1045, 1673
HpaII	CCGG	6 cut(s)	159, 216, 248, 374, 482, 1405
HphI	GGTGA	3 cut(s)	46, 611, 913
Hpy166II	GTNNAC	3 cut(s)	1250, 1402, 1639
Hpy188III	TCNNGA	9 cut(s)	36, 84, 302, 421, 686, 980, 1067, 1347, 1466
Hpy8I	GTNNAC	3 cut(s)	1250, 1402, 1639
Hpy99I	CGWCG	2 cut(s)	157, 260
HpyAV	CCTTC	9 cut(s)	181, 418, 703, 1003, 1054, 1121, 1407, 1551, 1685
HpyCH4IV	ACGT	2 cut(s)	1276, 1448
HpyF10VI	GCNNNNNNNGC	9 cut(s)	239, 243, 291, 548, 677, 1208, 1580, 1601, 1706
HpyF3I	CTNAG	8 cut(s)	23, 264, 732, 744, 978, 1047, 1068, 1345
HpySE526I	ACGT	2 cut(s)	1276, 1448
Hsp92II	CATG	9 cut(s)	298, 424, 562, 877, 1226, 1241, 1361, 1390, 1666
HspAI	GCGC	2 cut(s)	219, 595
Ksp22I	TGATCA	1 cut(s)	1084
Kzo9I	GATC	9 cut(s)	97, 381, 796, 963, 1084, 1372, 1430, 1462, 1514
LguI	GCTCTTC	2 cut(s)	1239, 1417
LmnI	GCTCC	5 cut(s)	210, 218, 370, 376, 631
Lsp1109I	GCAGC	6 cut(s)	529, 1255, 1567, 1586, 1589, 1709
LweI	GCATC	6 cut(s)	426, 689, 894, 934, 1124, 1498
MaeI	CTAG	6 cut(s)	92, 414, 611, 633, 828, 1596
MaeII	ACGT	2 cut(s)	1276, 1448
MaeIII	GTNAC	2 cut(s)	1042, 1184
MalI	GATC	9 cut(s)	99, 383, 798, 965, 1086, 1374, 1432, 1464, 1516
MboI	GATC	9 cut(s)	97, 381, 796, 963, 1084, 1372, 1430, 1462, 1514
MfeI	CAATTG	1 cut(s)	705
MflI	RGATCY	2 cut(s)	1372, 1514
MhlI	GDGCHC	3 cut(s)	35, 215, 244
MlsI	TGGCCA	1 cut(s)	1137
MluCI	AATT	6 cut(s)	334, 651, 705, 831, 971, 1366
MluNI	TGGCCA	1 cut(s)	1137
MlyI	GAGTC	4 cut(s)	353, 395, 698, 1039
MmeI	TCCRAC	4 cut(s)	150, 303, 381, 653
Mox20I	TGGCCA	1 cut(s)	1137
MscI	TGGCCA	1 cut(s)	1137
MseI	TTAA	2 cut(s)	333, 1151
MslI	CAYNNNNRTG	2 cut(s)	192, 557
Msp20I	TGGCCA	1 cut(s)	1137
MspA1I	CMGCKG	1 cut(s)	1580
MspI	CCGG	6 cut(s)	159, 216, 248, 374, 482, 1405
MspR9I	CCNGG	3 cut(s)	230, 248, 783
MunI	CAATTG	1 cut(s)	705
Mva1269I	GAATGC	3 cut(s)	545, 870, 1528
MvaI	CCWGG	2 cut(s)	230, 783
MvnI	CGCG	3 cut(s)	285, 597, 1332
MwoI	GCNNNNNNNGC	9 cut(s)	239, 243, 291, 548, 677, 1208, 1580, 1601, 1706
NciI	CCSGG	1 cut(s)	248
NdeII	GATC	9 cut(s)	97, 381, 796, 963, 1084, 1372, 1430, 1462, 1514
NheI	GCTAGC	1 cut(s)	610
NlaIII	CATG	9 cut(s)	298, 424, 562, 877, 1226, 1241, 1361, 1390, 1666
NlaIV	GGNNCC	5 cut(s)	378, 627, 721, 911, 1065
NmuCI	GTSAC	2 cut(s)	1042, 1184
NspI	RCATGY	2 cut(s)	1226, 1361
NspV	TTCGAA	1 cut(s)	1676
PaeI	GCATGC	1 cut(s)	1226
PaeR7I	CTCGAG	1 cut(s)	686
PagI	TCATGA	1 cut(s)	420
PciI	ACATGT	1 cut(s)	1357
PciSI	GCTCTTC	2 cut(s)	1239, 1417
PctI	GAATGC	3 cut(s)	545, 870, 1528
PfeI	GAWTC	3 cut(s)	251, 298, 1673
PflFI	GACNNNGTC	1 cut(s)	284
PfoI	TCCNGGA	1 cut(s)	781
PkrI	GCNGC	7 cut(s)	330, 544, 1270, 1576, 1579, 1582, 1699
Ple19I	CGATCG	1 cut(s)	384
PleI	GAGTC	4 cut(s)	353, 394, 697, 1039
PpsI	GAGTC	4 cut(s)	353, 394, 697, 1039
Ppu21I	YACGTR	1 cut(s)	1277
PscI	ACATGT	1 cut(s)	1357
Psp124BI	GAGCTC	3 cut(s)	35, 215, 244
Psp6I	CCWGG	2 cut(s)	228, 781
PspGI	CCWGG	2 cut(s)	228, 781
PspN4I	GGNNCC	5 cut(s)	378, 627, 721, 911, 1065
PspPI	GGNCC	2 cut(s)	605, 1625
PstNI	CAGNNNCTG	3 cut(s)	1511, 1580, 1583
PsuI	RGATCY	2 cut(s)	1372, 1514
PsyI	GACNNNGTC	1 cut(s)	284
PvuI	CGATCG	1 cut(s)	384
PvuII	CAGCTG	1 cut(s)	1580
RsaI	GTAC	2 cut(s)	1249, 1401
RsaNI	GTAC	2 cut(s)	1248, 1400
RseI	CAYNNNNRTG	2 cut(s)	192, 557
SacI	GAGCTC	3 cut(s)	35, 215, 244
SapI	GCTCTTC	2 cut(s)	1239, 1417
SaqAI	TTAA	2 cut(s)	333, 1151
SatI	GCNGC	7 cut(s)	329, 543, 1269, 1575, 1578, 1581, 1698
Sau3AI	GATC	9 cut(s)	97, 381, 796, 963, 1084, 1372, 1430, 1462, 1514
Sau96I	GGNCC	2 cut(s)	605, 1625
SchI	GAGTC	4 cut(s)	353, 395, 698, 1039
ScrFI	CCNGG	3 cut(s)	230, 248, 783
SduI	GDGCHC	3 cut(s)	35, 215, 244
SfaNI	GCATC	6 cut(s)	426, 689, 894, 934, 1124, 1498
SfcI	CTRYAG	1 cut(s)	1032
Sfr274I	CTCGAG	1 cut(s)	686
SfuI	TTCGAA	1 cut(s)	1676
SinI	GGWCC	2 cut(s)	605, 1625
SlaI	CTCGAG	1 cut(s)	686
SmiMI	CAYNNNNRTG	2 cut(s)	192, 557
SmlI	CTYRAG	3 cut(s)	34, 471, 686
SmoI	CTYRAG	3 cut(s)	34, 471, 686
SphI	GCATGC	1 cut(s)	1226
Sse9I	AATT	6 cut(s)	334, 651, 705, 831, 971, 1366
SsiI	CCGC	9 cut(s)	17, 283, 292, 328, 597, 608, 907, 1332, 1670
SspMI	CTAG	6 cut(s)	92, 414, 611, 633, 828, 1596
SstI	GAGCTC	3 cut(s)	35, 215, 244
StyD4I	CCNGG	3 cut(s)	228, 246, 781
TaiI	ACGT	2 cut(s)	1279, 1451
TaqI	TCGA	7 cut(s)	29, 85, 384, 432, 687, 966, 1676
TasI	AATT	6 cut(s)	334, 651, 705, 831, 971, 1366
TatI	WGTACW	1 cut(s)	1399
TauI	GCSGC	1 cut(s)	331
TfiI	GAWTC	3 cut(s)	251, 298, 1673
Tru1I	TTAA	2 cut(s)	333, 1151
Tru9I	TTAA	2 cut(s)	333, 1151
TscAI	CASTG	5 cut(s)	73, 272, 499, 535, 1321
TseFI	GTSAC	2 cut(s)	1042, 1184
TseI	GCWGC	6 cut(s)	542, 1268, 1574, 1577, 1580, 1697
Tsp45I	GTSAC	2 cut(s)	1042, 1184
TspDTI	ATGAA	5 cut(s)	311, 437, 867, 1226, 1651
TspGWI	ACGGA	2 cut(s)	244, 456
TspRI	CASTG	5 cut(s)	73, 272, 499, 535, 1321
Tth111I	GACNNNGTC	1 cut(s)	284
VpaK11BI	GGWCC	2 cut(s)	605, 1625
XapI	RAATTY	1 cut(s)	831
XceI	RCATGY	2 cut(s)	1226, 1361
XhoI	CTCGAG	1 cut(s)	686
XspI	CTAG	6 cut(s)	92, 414, 611, 633, 828, 1596

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.