Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0011549

Physical Location & Seq

Genomic Coordinates Forward (+)

78285191 .. 78291322

Gene Structure 6132 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G044590.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1710 bp

ATGTCGTATGTGCCTCCTCACCTGAGAAACTCAACCTCAACCACCACCACCACCACCACCACTACCACCGCCACCGCCAGAACCTCCTCCGGAACCGACTCGTCCGCTGCTTCATTCTCCTCGTTCTCGCACTCCAATGGGTCCCGGTGGAGCTCCGCCGCTCACTCTCCGGCCAACCGAGCTATCTCCCCGCCCGACGCCGTCGTCCCTCAGTGGAAGCCGTCGGAACGGGTCCTCCGAATGAAGCCGGAACTGATTGAGGAGGTGTGTTTGCGGCTTAATTTGGATGTTTCTGTTAGTCCAGACTCTGCTCCTCCTCCGGCACCAATTGAGTCTTTTACAGATATGTGTTTGCACCCTAGCATTATGAAGGATATAGCATTTCATGGATACACTATGCCAACACCAATTCAAGCTCAAGCCATGCCAGTTGCCCTCAGTGGAAGGGATTTATTAGGTTGCGCTGAAACTGGTAGTGGTAAAACAGCTGCATTTGCCATTCCTATGATACAGCATTGTTTGGCTCAACCTCCCGTTCAGCGTGGTGATGGACCACTAGCATTGGTACTGGCCCCAACTAGAGAGCTTGCACAACAAATAGAGGAAGAGGTCAAGGCTTTTAGTAAATCTCTCGAGTCCTTCAAAACTGCAATAGTTGTGGGTGGAACCAACATAGCTGAGCAGAGATCTGAGCTTCGAGCAGGAGTTAATATTGTGGTTGCTACTCCTGGAAGATTCATAGATCATTTACAGCAAGGGAACACTTCTGTGTCTAGAATTTCGTTTGTTGTTCTAGATGAAGCTGATAGAATGCTTGACATGGGTTTTGAACCACAGATAAGAGAGGTGATGCGGAACCTTTCTGAAAAGCATCAAACTTTGCTGTTTAGTGCAACTATGCCTGAAGAGATTGAAGGGTTAGCCCAGGAATATTTGACTAATCCAGTGCAAGTGAAGGTGGGAAAAGTGAGTAGCCCTACAGCAAATGTAACTCAGAGTTTGGAGAAGGTTCCTGACAGTGAGAAGACTGATCGGCTTCTGGGTTTGCTCGTAGAGGAGTCATCTCGAGCTGAAAGAGCTGGACATCTATTTCCCTTAACTATTGTATTTGTTGAAAGGAAGACACGCTGTGATGATGTAGCTGAAGCTTTGGTAGCACAAGGTTTGCATGCTGTTGCTCTTCATGGTGGACGTACTCAAACTGAAAGAGAGGCTGCTCTGCGTGAATTTAGAAAAGGCACTACCAACATCTTGGTTGCCACCGATGTTGCATCTCGTGGTTTGGACGTCTCAGGAGTTGCTCACGTTATCAATTTGGATCTTCCGAAGAGCATGGAAGATTATGTACACCGGATTGGAAGGACTGGAAGAGCGGGATCAATGGGACAAGCTACTTCCTTCTACACTGATCGTGATATGTTCCTTGTGGCGAATATAAAGAAAGCAATAGCTGATGTGGGATCAGGGAATGCTCTGCTTTTGCTACGGGGAAGGTACAGCTTTTTTGCTATTTATGAACAGTCAATTCTTGACACTGCAAGAAGGAAAGAAAGAGAAGCAGCTGCTGCTCACAAGCAAGCTAGGGTTTCTCTATCCAAGTCTTCAAGTATGGGGCCCACATCAATAAACATTGAGGACAAGTATAAGTTCATGGTTTCTGAACCGGACAATCAAACAGAGGGTGCAGCAGATAGTGCTTGGGACGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko03040 ko05202 ko05205 map03040 map05202 map05205

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

569

Amino Acids

61.66

Weight (kDa)

6.01

Isoelectric Point (pI)

48.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	135 - 305	3.6e-53	DEAD/DEAH box helicase
ResIII	PF04851	155 - 301	5.8e-09	Type III restriction enzyme, res subunit
Helicase_C	PF00271	367 - 459	1e-29	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011549)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g40600 FvH4_3g40620
malus_domestica	MD03G1050800.v1.1 MD11G1052800.v1.1
prunus_persica	Prupe.6G040300_v2.0.a1 Prupe.6G040300_v2.0.a1
pyrus_communis	pycom03g04010 pycom11g04300
rosa_chinensis	RchiOBHm_Chr5g0073371
rosa_laevigata	RLG00000036381
rosa_multiflora	Rmu_sc0024948.1_g000001
rosa_roxburghii	Rroxscaffold_1G00007870
rosa_rugosa	Rorug05G0423000 Rorug05G0423100 Rorug05G0423200
rosa_samantha	Rh5AG479600 Rh5BG500800 Rh5CG523900 Rh5DG512700
rosa_wichuraiana	Rw5G044590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	203
AatII	GACGTC	1 cut(s)	1290
AccB1I	GGYRCC	1 cut(s)	322
AccBSI	CCGCTC	2 cut(s)	161, 1373
AccIII	TCCGGA	1 cut(s)	89
AciI	CCGC	9 cut(s)	69, 75, 105, 156, 159, 191, 274, 853, 1373
AclWI	GGATC	3 cut(s)	1326, 1384, 1468
AcoI	YGGCCR	1 cut(s)	171
AcsI	RAATTY	2 cut(s)	777, 1226
AcuI	CTGAAG	2 cut(s)	924, 1164
AcyI	GRCGYC	2 cut(s)	198, 1287
AdeI	CACNNNGTG	1 cut(s)	1130
AfaI	GTAC	4 cut(s)	567, 1195, 1347, 1496
AgsI	TTSAA	6 cut(s)	413, 643, 830, 914, 1115, 1605
AjnI	CCWGG	2 cut(s)	727, 924
AleI	CACNNNNGTG	1 cut(s)	767
AloI	GAACNNNNNNTCC	2 cut(s)	219, 251
Alw21I	GWGCWC	1 cut(s)	155
Alw26I	GTCTC	1 cut(s)	1294
AlwI	GGATC	3 cut(s)	1326, 1384, 1468
AlwNI	CAGNNNCTG	2 cut(s)	308, 1565
Ama87I	CYCGRG	2 cut(s)	632, 1065
Aor13HI	TCCGGA	1 cut(s)	89
AoxI	GGCC	3 cut(s)	171, 570, 1613
ApaI	GGGCCC	1 cut(s)	1617
ApeKI	GCWGC	7 cut(s)	107, 488, 1214, 1559, 1562, 1565, 1685
ApoI	RAATTY	2 cut(s)	777, 1226
ArsI	GACNNNNNNTTYG	2 cut(s)	809, 841
AspLEI	GCGC	1 cut(s)	464
AspS9I	GGNCC	6 cut(s)	141, 232, 551, 571, 1613, 1614
AsuC2I	CCSGG	1 cut(s)	145
AsuHPI	GGTGA	3 cut(s)	11, 557, 859
AvaI	CYCGRG	2 cut(s)	632, 1065
AvaII	GGWCC	3 cut(s)	141, 232, 551
BaeGI	GKGCMC	1 cut(s)	1617
BanI	GGYRCC	1 cut(s)	322
BanII	GRGCYC	2 cut(s)	155, 1617
BarI	GAAGNNNNNNTAC	2 cut(s)	1329, 1361
BauI	CACGAG	1 cut(s)	1275
BbsI	GAAGAC	3 cut(s)	1031, 1127, 1593
Bbv12I	GWGCWC	1 cut(s)	155
BbvI	GCAGC	7 cut(s)	94, 475, 1201, 1549, 1552, 1571, 1697
BccI	CCATC	1 cut(s)	542
BceAI	ACGGC	2 cut(s)	185, 205
BciT130I	CCWGG	2 cut(s)	729, 926
BciVI	GTATCC	1 cut(s)	383
BcnI	CCSGG	1 cut(s)	145
BcoDI	GTCTC	1 cut(s)	1294
BfaI	CTAG	6 cut(s)	360, 557, 579, 774, 794, 1581
BfmI	CTRYAG	1 cut(s)	978
BfuI	GTATCC	1 cut(s)	383
BglII	AGATCT	1 cut(s)	686
BisI	GCNGC	9 cut(s)	108, 159, 275, 489, 1215, 1560, 1563, 1566, 1686
BlpI	GCTNAGC	1 cut(s)	678
BlsI	GCNGC	9 cut(s)	109, 160, 276, 490, 1216, 1561, 1564, 1567, 1687
Bme1390I	CCNGG	3 cut(s)	145, 729, 926
Bme18I	GGWCC	3 cut(s)	141, 232, 551
BmeT110I	CYCGRG	2 cut(s)	632, 1065
BmgT120I	GGNCC	6 cut(s)	141, 232, 551, 571, 1613, 1614
BmrFI	CCNGG	3 cut(s)	145, 729, 926
BmsI	GCATC	3 cut(s)	840, 880, 1280
BpiI	GAAGAC	3 cut(s)	1031, 1127, 1593
Bpu1102I	GCTNAGC	1 cut(s)	678
BpuEI	CTTGAG	1 cut(s)	402
BpuMI	CCSGG	1 cut(s)	145
BsaHI	GRCGYC	2 cut(s)	198, 1287
BsaJI	CCNNGG	1 cut(s)	924
BsaWI	WCCGGW	3 cut(s)	89, 1350, 1663
BsaXI	ACNNNNNCTCC	2 cut(s)	219, 249
Bse1I	ACTGG	5 cut(s)	428, 475, 573, 944, 1369
BseAI	TCCGGA	1 cut(s)	89
BseBI	CCWGG	2 cut(s)	729, 926
BseDI	CCNNGG	1 cut(s)	924
BseGI	GGATG	1 cut(s)	292
BseMII	CTCAG	7 cut(s)	14, 224, 451, 669, 681, 1007, 1305
BseNI	ACTGG	5 cut(s)	428, 475, 573, 944, 1369
BseRI	GAGGAG	7 cut(s)	6, 76, 109, 275, 303, 306, 1070
BseSI	GKGCMC	1 cut(s)	1617
BseXI	GCAGC	7 cut(s)	94, 475, 1201, 1549, 1552, 1571, 1697
BsgI	GTGCAG	1 cut(s)	1704
BshFI	GGCC	3 cut(s)	173, 572, 1615
BshNI	GGYRCC	1 cut(s)	322
BsiHKAI	GWGCWC	1 cut(s)	155
BsiHKCI	CYCGRG	2 cut(s)	632, 1065
BsiSI	CCGG	7 cut(s)	90, 145, 170, 248, 320, 1351, 1664
BslFI	GGGAC	3 cut(s)	127, 191, 1398
BsmAI	GTCTC	1 cut(s)	1294
BsmBI	CGTCTC	1 cut(s)	1294
BsmFI	GGGAC	3 cut(s)	127, 191, 1398
BsmI	GAATGC	2 cut(s)	816, 1474
BsnI	GGCC	3 cut(s)	173, 572, 1615
BsoBI	CYCGRG	2 cut(s)	632, 1065
Bsp120I	GGGCCC	1 cut(s)	1613
Bsp1286I	GDGCHC	2 cut(s)	155, 1617
Bsp13I	TCCGGA	1 cut(s)	89
Bsp1407I	TGTACA	1 cut(s)	1345
Bsp143I	GATC	7 cut(s)	686, 742, 1030, 1318, 1376, 1408, 1460
Bsp1720I	GCTNAGC	1 cut(s)	678
BspACI	CCGC	9 cut(s)	69, 75, 105, 156, 159, 191, 274, 853, 1373
BspANI	GGCC	3 cut(s)	173, 572, 1615
BspCNI	CTCAG	7 cut(s)	15, 223, 450, 670, 682, 1006, 1304
BspEI	TCCGGA	1 cut(s)	89
BspPI	GGATC	3 cut(s)	1326, 1384, 1468
BspQI	GCTCTTC	3 cut(s)	1185, 1322, 1363
BspT107I	GGYRCC	1 cut(s)	322
BsrBI	CCGCTC	2 cut(s)	161, 1373
BsrGI	TGTACA	1 cut(s)	1345
BsrI	ACTGG	5 cut(s)	428, 475, 573, 944, 1369
BssECI	CCNNGG	1 cut(s)	924
BssMI	GATC	7 cut(s)	686, 742, 1030, 1318, 1376, 1408, 1460
BssNI	GRCGYC	2 cut(s)	198, 1287
BssSI	CACGAG	1 cut(s)	1275
Bst2BI	CACGAG	1 cut(s)	1275
Bst2UI	CCWGG	2 cut(s)	729, 926
Bst4CI	ACNGT	2 cut(s)	1019, 1521
Bst6I	CTCTTC	5 cut(s)	600, 900, 1185, 1322, 1363
BstACI	GRCGYC	2 cut(s)	198, 1287
BstAPI	GCANNNNNTGC	2 cut(s)	1565, 1694
BstAUI	TGTACA	1 cut(s)	1345
BstC8I	GCNNGC	3 cut(s)	588, 1170, 1578
BstDEI	CTNAG	7 cut(s)	23, 210, 437, 678, 690, 993, 1291
BstF5I	GGATG	1 cut(s)	292
BstHHI	GCGC	1 cut(s)	464
BstKTI	GATC	7 cut(s)	689, 745, 1033, 1321, 1379, 1411, 1463
BstMAI	GTCTC	1 cut(s)	1294
BstMBI	GATC	7 cut(s)	686, 742, 1030, 1318, 1376, 1408, 1460
BstMWI	GCNNNNNNNGC	6 cut(s)	179, 494, 1076, 1154, 1565, 1694
BstNI	CCWGG	2 cut(s)	729, 926
BstNSI	RCATGY	1 cut(s)	1172
BstSCI	CCNGG	3 cut(s)	143, 727, 924
BstSFI	CTRYAG	1 cut(s)	978
BstSLI	GKGCMC	1 cut(s)	1617
BstV1I	GCAGC	7 cut(s)	94, 475, 1201, 1549, 1552, 1571, 1697
BstV2I	GAAGAC	3 cut(s)	1031, 1127, 1593
BstX2I	RGATCY	2 cut(s)	686, 1318
BstXI	CCANNNNNNTGG	1 cut(s)	1252
BstYI	RGATCY	2 cut(s)	686, 1318
BsuI	GTATCC	1 cut(s)	383
BsuRI	GGCC	3 cut(s)	173, 572, 1615
BtsCI	GGATG	1 cut(s)	292
BtsI	GCAGTG	1 cut(s)	1533
BtsIMutI	CAGTG	6 cut(s)	218, 445, 951, 1024, 1404, 1533
Cac8I	GCNNGC	3 cut(s)	588, 1170, 1578
CaiI	CAGNNNCTG	2 cut(s)	308, 1565
CfoI	GCGC	1 cut(s)	464
Cfr13I	GGNCC	6 cut(s)	141, 232, 551, 571, 1613, 1614
CseI	GACGC	1 cut(s)	206
Csp6I	GTAC	4 cut(s)	566, 1194, 1346, 1495
CspCI	CAANNNNNGTGG	6 cut(s)	543, 578, 639, 674, 1249, 1284
CviAII	CATG	7 cut(s)	386, 424, 820, 1169, 1184, 1333, 1651
CviQI	GTAC	4 cut(s)	566, 1194, 1346, 1495
DdeI	CTNAG	7 cut(s)	23, 210, 437, 678, 690, 993, 1291
DpnI	GATC	7 cut(s)	688, 744, 1032, 1320, 1378, 1410, 1462
DpnII	GATC	7 cut(s)	686, 742, 1030, 1318, 1376, 1408, 1460
DraIII	CACNNNGTG	1 cut(s)	1130
DrdI	GACNNNNNNGTC	1 cut(s)	203
DseDI	GACNNNNNNGTC	1 cut(s)	203
EaeI	YGGCCR	1 cut(s)	171
Eam1104I	CTCTTC	5 cut(s)	600, 900, 1185, 1322, 1363
EarI	CTCTTC	5 cut(s)	600, 900, 1185, 1322, 1363
EciI	GGCGGA	1 cut(s)	145
Ecl136II	GAGCTC	1 cut(s)	153
Eco24I	GRGCYC	2 cut(s)	155, 1617
Eco47I	GGWCC	3 cut(s)	141, 232, 551
Eco53kI	GAGCTC	1 cut(s)	153
Eco57I	CTGAAG	2 cut(s)	924, 1164
Eco88I	CYCGRG	2 cut(s)	632, 1065
EcoICRI	GAGCTC	1 cut(s)	153
EcoO109I	RGGNCCY	3 cut(s)	141, 232, 1613
EcoRII	CCWGG	2 cut(s)	727, 924
EcoT38I	GRGCYC	2 cut(s)	155, 1617
Esp3I	CGTCTC	1 cut(s)	1294
FaeI	CATG	7 cut(s)	389, 427, 823, 1172, 1187, 1336, 1654
FaqI	GGGAC	3 cut(s)	127, 191, 1398
FatI	CATG	7 cut(s)	385, 423, 819, 1168, 1183, 1332, 1650
FauI	CCCGC	2 cut(s)	198, 1366
Fnu4HI	GCNGC	9 cut(s)	108, 159, 275, 489, 1215, 1560, 1563, 1566, 1686
FokI	GGATG	1 cut(s)	299
FriOI	GRGCYC	2 cut(s)	155, 1617
Fsp4HI	GCNGC	9 cut(s)	108, 159, 275, 489, 1215, 1560, 1563, 1566, 1686
FspBI	CTAG	6 cut(s)	360, 557, 579, 774, 794, 1581
GlaI	GCGC	1 cut(s)	463
GluI	GCNGC	9 cut(s)	108, 159, 275, 489, 1215, 1560, 1563, 1566, 1686
HaeIII	GGCC	3 cut(s)	173, 572, 1615
HapII	CCGG	7 cut(s)	90, 145, 170, 248, 320, 1351, 1664
HgaI	GACGC	1 cut(s)	206
HhaI	GCGC	1 cut(s)	464
Hin1I	GRCGYC	2 cut(s)	198, 1287
Hin1II	CATG	7 cut(s)	389, 427, 823, 1172, 1187, 1336, 1654
Hin6I	GCGC	1 cut(s)	462
HinP1I	GCGC	1 cut(s)	462
HindIII	AAGCTT	1 cut(s)	1146
HinfI	GANTC	6 cut(s)	98, 305, 332, 635, 735, 1058
HpaII	CCGG	7 cut(s)	90, 145, 170, 248, 320, 1351, 1664
HphI	GGTGA	3 cut(s)	11, 557, 859
Hpy166II	GTNNAC	2 cut(s)	1190, 1348
Hpy188I	TCNGA	7 cut(s)	226, 239, 691, 865, 996, 1326, 1660
Hpy8I	GTNNAC	2 cut(s)	1190, 1348
Hpy99I	CGWCG	3 cut(s)	200, 206, 226
HpyCH4III	ACNGT	2 cut(s)	1019, 1521
HpyCH4IV	ACGT	3 cut(s)	1192, 1287, 1305
HpyF10VI	GCNNNNNNNGC	6 cut(s)	179, 494, 1076, 1154, 1565, 1694
HpyF3I	CTNAG	7 cut(s)	23, 210, 437, 678, 690, 993, 1291
HpySE526I	ACGT	3 cut(s)	1192, 1287, 1305
Hsp92I	GRCGYC	2 cut(s)	198, 1287
Hsp92II	CATG	7 cut(s)	389, 427, 823, 1172, 1187, 1336, 1654
HspAI	GCGC	1 cut(s)	462
KflI	GGGWCCC	1 cut(s)	141
Kpn2I	TCCGGA	1 cut(s)	89
Kzo9I	GATC	7 cut(s)	686, 742, 1030, 1318, 1376, 1408, 1460
LguI	GCTCTTC	3 cut(s)	1185, 1322, 1363
LmnI	GCTCC	3 cut(s)	150, 158, 316
Lsp1109I	GCAGC	7 cut(s)	94, 475, 1201, 1549, 1552, 1571, 1697
LweI	GCATC	3 cut(s)	840, 880, 1280
MaeI	CTAG	6 cut(s)	360, 557, 579, 774, 794, 1581
MaeII	ACGT	3 cut(s)	1192, 1287, 1305
MaeIII	GTNAC	1 cut(s)	988
MalI	GATC	7 cut(s)	688, 744, 1032, 1320, 1378, 1410, 1462
MbiI	CCGCTC	2 cut(s)	161, 1373
MboI	GATC	7 cut(s)	686, 742, 1030, 1318, 1376, 1408, 1460
MfeI	CAATTG	1 cut(s)	327
MflI	RGATCY	2 cut(s)	686, 1318
MhlI	GDGCHC	2 cut(s)	155, 1617
MluCI	AATT	7 cut(s)	280, 327, 408, 777, 1226, 1312, 1524
MlyI	GAGTC	5 cut(s)	92, 299, 341, 644, 1067
MmeI	TCCRAC	1 cut(s)	204
MroI	TCCGGA	1 cut(s)	89
MseI	TTAA	3 cut(s)	279, 708, 1097
MslI	CAYNNNNRTG	3 cut(s)	135, 503, 767
MspA1I	CMGCKG	3 cut(s)	107, 488, 1562
MspI	CCGG	7 cut(s)	90, 145, 170, 248, 320, 1351, 1664
MspR9I	CCNGG	3 cut(s)	145, 729, 926
MunI	CAATTG	1 cut(s)	327
Mva1269I	GAATGC	2 cut(s)	816, 1474
MvaI	CCWGG	2 cut(s)	729, 926
MwoI	GCNNNNNNNGC	6 cut(s)	179, 494, 1076, 1154, 1565, 1694
NciI	CCSGG	1 cut(s)	145
NdeII	GATC	7 cut(s)	686, 742, 1030, 1318, 1376, 1408, 1460
NlaIII	CATG	7 cut(s)	389, 427, 823, 1172, 1187, 1336, 1654
NspI	RCATGY	1 cut(s)	1172
OliI	CACNNNNGTG	1 cut(s)	767
PaeI	GCATGC	1 cut(s)	1172
PaeR7I	CTCGAG	2 cut(s)	632, 1065
PciSI	GCTCTTC	3 cut(s)	1185, 1322, 1363
PcsI	WCGNNNNNNNCGW	1 cut(s)	235
PctI	GAATGC	2 cut(s)	816, 1474
PfeI	GAWTC	1 cut(s)	735
PflFI	GACNNNGTC	1 cut(s)	200
PfoI	TCCNGGA	1 cut(s)	727
PkrI	GCNGC	9 cut(s)	109, 160, 276, 490, 1216, 1561, 1564, 1567, 1687
PleI	GAGTC	5 cut(s)	92, 299, 340, 643, 1066
PpsI	GAGTC	5 cut(s)	92, 299, 340, 643, 1066
PpuMI	RGGWCCY	2 cut(s)	141, 232
Psp124BI	GAGCTC	1 cut(s)	155
Psp5II	RGGWCCY	2 cut(s)	141, 232
Psp6I	CCWGG	2 cut(s)	727, 924
PspGI	CCWGG	2 cut(s)	727, 924
PspOMI	GGGCCC	1 cut(s)	1613
PspPI	GGNCC	6 cut(s)	141, 232, 551, 571, 1613, 1614
PspPPI	RGGWCCY	2 cut(s)	141, 232
PstNI	CAGNNNCTG	2 cut(s)	308, 1565
PsuI	RGATCY	2 cut(s)	686, 1318
PsyI	GACNNNGTC	1 cut(s)	200
PvuII	CAGCTG	2 cut(s)	488, 1562
RsaI	GTAC	4 cut(s)	567, 1195, 1347, 1496
RsaNI	GTAC	4 cut(s)	566, 1194, 1346, 1495
RseI	CAYNNNNRTG	3 cut(s)	135, 503, 767
SacI	GAGCTC	1 cut(s)	155
SapI	GCTCTTC	3 cut(s)	1185, 1322, 1363
SaqAI	TTAA	3 cut(s)	279, 708, 1097
SatI	GCNGC	9 cut(s)	108, 159, 275, 489, 1215, 1560, 1563, 1566, 1686
Sau3AI	GATC	7 cut(s)	686, 742, 1030, 1318, 1376, 1408, 1460
Sau96I	GGNCC	6 cut(s)	141, 232, 551, 571, 1613, 1614
SchI	GAGTC	5 cut(s)	92, 299, 341, 644, 1067
ScrFI	CCNGG	3 cut(s)	145, 729, 926
SduI	GDGCHC	2 cut(s)	155, 1617
SfaNI	GCATC	3 cut(s)	840, 880, 1280
SfcI	CTRYAG	1 cut(s)	978
Sfr274I	CTCGAG	2 cut(s)	632, 1065
SinI	GGWCC	3 cut(s)	141, 232, 551
SlaI	CTCGAG	2 cut(s)	632, 1065
SmiMI	CAYNNNNRTG	3 cut(s)	135, 503, 767
SmlI	CTYRAG	3 cut(s)	417, 632, 1065
SmoI	CTYRAG	3 cut(s)	417, 632, 1065
SphI	GCATGC	1 cut(s)	1172
Sse9I	AATT	7 cut(s)	280, 327, 408, 777, 1226, 1312, 1524
SsiI	CCGC	9 cut(s)	69, 75, 105, 156, 159, 191, 274, 853, 1373
SspI	AATATT	2 cut(s)	712, 932
SspMI	CTAG	6 cut(s)	360, 557, 579, 774, 794, 1581
SstI	GAGCTC	1 cut(s)	155
StyD4I	CCNGG	3 cut(s)	143, 727, 924
TaaI	ACNGT	2 cut(s)	1019, 1521
TaiI	ACGT	3 cut(s)	1195, 1290, 1308
TaqI	TCGA	3 cut(s)	633, 697, 1066
TasI	AATT	7 cut(s)	280, 327, 408, 777, 1226, 1312, 1524
TatI	WGTACW	1 cut(s)	1345
TauI	GCSGC	2 cut(s)	161, 277
TfiI	GAWTC	1 cut(s)	735
Tru1I	TTAA	3 cut(s)	279, 708, 1097
Tru9I	TTAA	3 cut(s)	279, 708, 1097
TscAI	CASTG	6 cut(s)	218, 445, 951, 1024, 1411, 1540
TseI	GCWGC	7 cut(s)	107, 488, 1214, 1559, 1562, 1565, 1685
TspDTI	ATGAA	9 cut(s)	102, 257, 374, 383, 727, 813, 1172, 1530, 1639
TspRI	CASTG	6 cut(s)	218, 445, 951, 1024, 1411, 1540
Tth111I	GACNNNGTC	1 cut(s)	200
VpaK11BI	GGWCC	3 cut(s)	141, 232, 551
XapI	RAATTY	2 cut(s)	777, 1226
XbaI	TCTAGA	2 cut(s)	773, 793
XceI	RCATGY	1 cut(s)	1172
XhoI	CTCGAG	2 cut(s)	632, 1065
XspI	CTAG	6 cut(s)	360, 557, 579, 774, 794, 1581
ZraI	GACGTC	1 cut(s)	1288

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.