Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0011549

Physical Location & Seq

Genomic Coordinates Forward (+)

82379725 .. 82385979

Gene Structure 6255 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG479600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1734 bp

ATGTCGTATGTGCCTCCTCACCTGAGAAACTCAACCTCAACCACCACCACCACCACCACTACCACCGCCAGCGCCAGCGCCAGAACCTCCTCCGGAACCGACGCTGATCGGAGCAAGCTCTCTTACTCCTCCTCCAACTCTCACTCCAACAACAACGAAAAGTCGTCCGCTGCTTCATTCTCCTCGTTCTCGCACTCCAATGGCTCCCGGTGGAGCTCCGCCGCTCACTCGCCGGCCAACCGAGCTATCTCCCCGCCCGACGCCGTCGTCCCTCAGTGGAAGCCGTCGGAACGGGTCCTCCGAATGAAGCCGGAACTGATTGAGGAGGTGTGTTTGCGGCTTAATTTGGATGTTTCTGTTAGTCCTGACTCTGCTCCTCCCCCGGCACCAATTGAGTCTTTTACAGATATGTGTTTGCACCCTAGCATTATGAAGGATATAGCATTTCATGGATACACTATGCCAACACCAATTCAAGCTCAAGCCATGCCAGTTGCCCTCAGTGGAAGGGATTTATTAGGTTGCGCTGAAACTGGTAGTGGTAAAACTGCTGCATTTGCCATTCCTATGATACAGCATTGTTTGGCTCAACCTCCTGTTCAGCGTGGTGATGGACCACTAGCATTGGTACTGGCCCCAACTAGAGAGCTTGCACAACAAATAGAGGAAGAGGTCAAGGCTTTTAGTAAATCTCTCGAGTCGTTCAAAACTGCAATAGTTGTGGGTGGAACCAACATAGCTGAGCAGAGATCTGAGCTTCGAGCAGGAGTTAATATTGTCGTTGCTACTCCTGGAAGATTCATAGATCATTTACAGCAAGGGAACACTTCTGTGTCTAGAATTTCGTTTGTTGTTCTAGATGAAGCTGATAGAATGCTTGACATGGGTTTTGAACCACAGATAAGAGAGGTGATGCGGAACCTTTCTGAAAAGCATCAAACTTTGCTGTTTAGTGCAACTATGCCTGAAGAGATTGAAGGGTTAGCCCAGGAATATTTGACTAATCCAGTGCAAGTGAAGGTGGGAAAAGTGAGTAGCCCTACAGCAAATGTAACTCAGAGTTTGGAGAAGGTTCCTGACAGTGAGAAGACTGATCGGCTTCTGGGTTTGCTCGTAGAGGAGTCATCTCGAGCTGAAAGAGCTGGACATCTATTTCCCTTAACTATTGTATTTGTTGAAAGGAAGACACGCTGTGATGATGTAGCTGAAGCTTTGGTAGCACAGGGTTTGCATGCTGTTGCTCTTCATGGTGGACGTACTCAAACTGAAAGAGAGGCTGCTCTGCGTGAATTTAGAAAAGGCACTACCAACATCTTGGTTGCCACCGATGTTGCATCTCGTGGTTTGGACGTCTCAGGAGTTGCTCACGTTATCAATTTGGATCTTCCGAAGAGCATGGAAGATTATGTACACCGGATTGGAAGGACTGGAAGAGCGGGATCAATGGGACAAGCTACTTCCTTCTACACTGATCGTGATATGTTCCTTGTGGCGAATATAAAGAAAGCAATAGCTGATGTGGGATCTGGGAATGCTCTAGCTTTTGCTACGGGGAAGACTGCAAGAAGGAAAGAAAGAGAAGCAGCTGCTGCTCACAAGCAAGCTAGGGTTTCTCTATCCAAGTCTTCAAGTATGGGGCCCACATCAATAAACATTGAGGACAAGTATAAGTTCATGGTTTCTGAACCGGACAATCAAACAGAGGGTGCAGCAGATAGTGCTTGGGACGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko03040 ko05202 ko05205 map03040 map05202 map05205

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

577

Amino Acids

62.2

Weight (kDa)

6.24

Isoelectric Point (pI)

47.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	156 - 326	3.7e-53	DEAD/DEAH box helicase
ResIII	PF04851	176 - 322	6e-09	Type III restriction enzyme, res subunit
Helicase_C	PF00271	388 - 480	1.1e-29	Helicase conserved C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011549)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g40600 FvH4_3g40620
malus_domestica	MD03G1050800.v1.1 MD11G1052800.v1.1
prunus_persica	Prupe.6G040300_v2.0.a1 Prupe.6G040300_v2.0.a1
pyrus_communis	pycom03g04010 pycom11g04300
rosa_chinensis	RchiOBHm_Chr5g0073371
rosa_laevigata	RLG00000036381
rosa_multiflora	Rmu_sc0024948.1_g000001
rosa_roxburghii	Rroxscaffold_1G00007870
rosa_rugosa	Rorug05G0423000 Rorug05G0423100 Rorug05G0423200
rosa_samantha	Rh5AG479600 Rh5BG500800 Rh5CG523900 Rh5DG512700
rosa_wichuraiana	Rw5G044590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	266
AatII	GACGTC	1 cut(s)	1353
AccB1I	GGYRCC	1 cut(s)	385
AccBSI	CCGCTC	2 cut(s)	224, 1436
AccIII	TCCGGA	1 cut(s)	92
AciI	CCGC	8 cut(s)	66, 168, 219, 222, 254, 337, 916, 1436
AclWI	GGATC	3 cut(s)	1389, 1447, 1531
AcoI	YGGCCR	1 cut(s)	234
AcsI	RAATTY	2 cut(s)	840, 1289
AcuI	CTGAAG	2 cut(s)	987, 1227
AcyI	GRCGYC	2 cut(s)	261, 1350
AdeI	CACNNNGTG	1 cut(s)	1193
AfaI	GTAC	3 cut(s)	630, 1258, 1410
AgsI	TTSAA	6 cut(s)	476, 706, 893, 977, 1178, 1629
AjnI	CCWGG	2 cut(s)	790, 987
AleI	CACNNNNGTG	1 cut(s)	830
AloI	GAACNNNNNNTCC	2 cut(s)	282, 314
Alw21I	GWGCWC	1 cut(s)	218
Alw26I	GTCTC	1 cut(s)	1357
AlwI	GGATC	3 cut(s)	1389, 1447, 1531
AlwNI	CAGNNNCTG	1 cut(s)	1589
Ama87I	CYCGRG	2 cut(s)	695, 1128
Aor13HI	TCCGGA	1 cut(s)	92
AoxI	GGCC	3 cut(s)	234, 633, 1637
ApaI	GGGCCC	1 cut(s)	1641
ApeKI	GCWGC	7 cut(s)	170, 551, 1277, 1583, 1586, 1589, 1709
ApoI	RAATTY	2 cut(s)	840, 1289
ArsI	GACNNNNNNTTYG	2 cut(s)	872, 904
AspLEI	GCGC	3 cut(s)	74, 80, 527
AspS9I	GGNCC	5 cut(s)	295, 614, 634, 1637, 1638
AsuC2I	CCSGG	2 cut(s)	208, 383
AsuHPI	GGTGA	3 cut(s)	11, 620, 922
AvaI	CYCGRG	2 cut(s)	695, 1128
AvaII	GGWCC	2 cut(s)	295, 614
BaeGI	GKGCMC	1 cut(s)	1641
BanI	GGYRCC	1 cut(s)	385
BanII	GRGCYC	2 cut(s)	218, 1641
BarI	GAAGNNNNNNTAC	2 cut(s)	1392, 1424
BauI	CACGAG	1 cut(s)	1338
BbsI	GAAGAC	4 cut(s)	1094, 1190, 1562, 1617
Bbv12I	GWGCWC	1 cut(s)	218
BbvI	GCAGC	7 cut(s)	157, 538, 1264, 1573, 1576, 1595, 1721
BccI	CCATC	1 cut(s)	605
BceAI	ACGGC	2 cut(s)	248, 268
BciT130I	CCWGG	2 cut(s)	792, 989
BciVI	GTATCC	1 cut(s)	446
BcnI	CCSGG	2 cut(s)	208, 383
BcoDI	GTCTC	1 cut(s)	1357
BfaI	CTAG	7 cut(s)	423, 620, 642, 837, 857, 1538, 1605
BfmI	CTRYAG	1 cut(s)	1041
BfoI	RGCGCY	2 cut(s)	75, 81
BfuI	GTATCC	1 cut(s)	446
BglII	AGATCT	1 cut(s)	749
BisI	GCNGC	9 cut(s)	171, 222, 338, 552, 1278, 1584, 1587, 1590, 1710
BlpI	GCTNAGC	1 cut(s)	741
BlsI	GCNGC	9 cut(s)	172, 223, 339, 553, 1279, 1585, 1588, 1591, 1711
Bme1390I	CCNGG	4 cut(s)	208, 383, 792, 989
Bme18I	GGWCC	2 cut(s)	295, 614
BmeT110I	CYCGRG	2 cut(s)	695, 1128
BmgT120I	GGNCC	5 cut(s)	295, 614, 634, 1637, 1638
BmrFI	CCNGG	4 cut(s)	208, 383, 792, 989
BmsI	GCATC	3 cut(s)	903, 943, 1343
BpiI	GAAGAC	4 cut(s)	1094, 1190, 1562, 1617
Bpu1102I	GCTNAGC	1 cut(s)	741
BpuEI	CTTGAG	1 cut(s)	465
BpuMI	CCSGG	2 cut(s)	208, 383
BsaHI	GRCGYC	2 cut(s)	261, 1350
BsaJI	CCNNGG	2 cut(s)	381, 987
BsaWI	WCCGGW	3 cut(s)	92, 1413, 1687
BsaXI	ACNNNNNCTCC	6 cut(s)	116, 128, 146, 158, 282, 312
Bse118I	RCCGGY	1 cut(s)	232
Bse1I	ACTGG	5 cut(s)	491, 538, 636, 1007, 1432
BseAI	TCCGGA	1 cut(s)	92
BseBI	CCWGG	2 cut(s)	792, 989
BseDI	CCNNGG	2 cut(s)	381, 987
BseGI	GGATG	1 cut(s)	355
BseMII	CTCAG	7 cut(s)	14, 287, 514, 732, 744, 1070, 1368
BseNI	ACTGG	5 cut(s)	491, 538, 636, 1007, 1432
BseRI	GAGGAG	8 cut(s)	6, 79, 118, 121, 172, 338, 366, 1133
BseSI	GKGCMC	1 cut(s)	1641
BseXI	GCAGC	7 cut(s)	157, 538, 1264, 1573, 1576, 1595, 1721
BsgI	GTGCAG	1 cut(s)	1728
BshFI	GGCC	3 cut(s)	236, 635, 1639
BshNI	GGYRCC	1 cut(s)	385
BsiHKAI	GWGCWC	1 cut(s)	218
BsiHKCI	CYCGRG	2 cut(s)	695, 1128
BsiSI	CCGG	7 cut(s)	93, 208, 233, 311, 383, 1414, 1688
BslFI	GGGAC	2 cut(s)	254, 1461
BsmAI	GTCTC	1 cut(s)	1357
BsmBI	CGTCTC	1 cut(s)	1357
BsmFI	GGGAC	2 cut(s)	254, 1461
BsmI	GAATGC	2 cut(s)	879, 1537
BsnI	GGCC	3 cut(s)	236, 635, 1639
BsoBI	CYCGRG	2 cut(s)	695, 1128
Bsp120I	GGGCCC	1 cut(s)	1637
Bsp1286I	GDGCHC	2 cut(s)	218, 1641
Bsp13I	TCCGGA	1 cut(s)	92
Bsp1407I	TGTACA	1 cut(s)	1408
Bsp143I	GATC	8 cut(s)	106, 749, 805, 1093, 1381, 1439, 1471, 1523
Bsp1720I	GCTNAGC	1 cut(s)	741
BspACI	CCGC	8 cut(s)	66, 168, 219, 222, 254, 337, 916, 1436
BspANI	GGCC	3 cut(s)	236, 635, 1639
BspCNI	CTCAG	7 cut(s)	15, 286, 513, 733, 745, 1069, 1367
BspEI	TCCGGA	1 cut(s)	92
BspPI	GGATC	3 cut(s)	1389, 1447, 1531
BspQI	GCTCTTC	3 cut(s)	1248, 1385, 1426
BspT107I	GGYRCC	1 cut(s)	385
BsrBI	CCGCTC	2 cut(s)	224, 1436
BsrFI	RCCGGY	1 cut(s)	232
BsrGI	TGTACA	1 cut(s)	1408
BsrI	ACTGG	5 cut(s)	491, 538, 636, 1007, 1432
BssAI	RCCGGY	1 cut(s)	232
BssECI	CCNNGG	2 cut(s)	381, 987
BssMI	GATC	8 cut(s)	106, 749, 805, 1093, 1381, 1439, 1471, 1523
BssNI	GRCGYC	2 cut(s)	261, 1350
BssSI	CACGAG	1 cut(s)	1338
Bst2BI	CACGAG	1 cut(s)	1338
Bst2UI	CCWGG	2 cut(s)	792, 989
Bst4CI	ACNGT	1 cut(s)	1082
Bst6I	CTCTTC	5 cut(s)	663, 963, 1248, 1385, 1426
BstACI	GRCGYC	2 cut(s)	261, 1350
BstAPI	GCANNNNNTGC	2 cut(s)	1589, 1718
BstAUI	TGTACA	1 cut(s)	1408
BstC8I	GCNNGC	7 cut(s)	70, 76, 116, 234, 651, 1233, 1602
BstDEI	CTNAG	7 cut(s)	23, 273, 500, 741, 753, 1056, 1354
BstF5I	GGATG	1 cut(s)	355
BstH2I	RGCGCY	2 cut(s)	75, 81
BstHHI	GCGC	3 cut(s)	74, 80, 527
BstKTI	GATC	8 cut(s)	109, 752, 808, 1096, 1384, 1442, 1474, 1526
BstMAI	GTCTC	1 cut(s)	1357
BstMBI	GATC	8 cut(s)	106, 749, 805, 1093, 1381, 1439, 1471, 1523
BstMWI	GCNNNNNNNGC	6 cut(s)	242, 557, 1139, 1217, 1589, 1718
BstNI	CCWGG	2 cut(s)	792, 989
BstNSI	RCATGY	1 cut(s)	1235
BstSCI	CCNGG	4 cut(s)	206, 381, 790, 987
BstSFI	CTRYAG	1 cut(s)	1041
BstSLI	GKGCMC	1 cut(s)	1641
BstV1I	GCAGC	7 cut(s)	157, 538, 1264, 1573, 1576, 1595, 1721
BstV2I	GAAGAC	4 cut(s)	1094, 1190, 1562, 1617
BstX2I	RGATCY	3 cut(s)	749, 1381, 1523
BstXI	CCANNNNNNTGG	1 cut(s)	1315
BstYI	RGATCY	3 cut(s)	749, 1381, 1523
BsuI	GTATCC	1 cut(s)	446
BsuRI	GGCC	3 cut(s)	236, 635, 1639
BtsCI	GGATG	1 cut(s)	355
BtsIMutI	CAGTG	5 cut(s)	281, 508, 1014, 1087, 1467
Cac8I	GCNNGC	7 cut(s)	70, 76, 116, 234, 651, 1233, 1602
CaiI	CAGNNNCTG	1 cut(s)	1589
CfoI	GCGC	3 cut(s)	74, 80, 527
Cfr10I	RCCGGY	1 cut(s)	232
Cfr13I	GGNCC	5 cut(s)	295, 614, 634, 1637, 1638
CseI	GACGC	2 cut(s)	110, 269
Csp6I	GTAC	3 cut(s)	629, 1257, 1409
CspCI	CAANNNNNGTGG	6 cut(s)	606, 641, 702, 737, 1312, 1347
CviAII	CATG	7 cut(s)	449, 487, 883, 1232, 1247, 1396, 1675
CviQI	GTAC	3 cut(s)	629, 1257, 1409
DdeI	CTNAG	7 cut(s)	23, 273, 500, 741, 753, 1056, 1354
DpnI	GATC	8 cut(s)	108, 751, 807, 1095, 1383, 1441, 1473, 1525
DpnII	GATC	8 cut(s)	106, 749, 805, 1093, 1381, 1439, 1471, 1523
DraIII	CACNNNGTG	1 cut(s)	1193
DrdI	GACNNNNNNGTC	1 cut(s)	266
DseDI	GACNNNNNNGTC	1 cut(s)	266
EaeI	YGGCCR	1 cut(s)	234
Eam1104I	CTCTTC	5 cut(s)	663, 963, 1248, 1385, 1426
EarI	CTCTTC	5 cut(s)	663, 963, 1248, 1385, 1426
EciI	GGCGGA	1 cut(s)	208
Ecl136II	GAGCTC	1 cut(s)	216
Eco24I	GRGCYC	2 cut(s)	218, 1641
Eco47I	GGWCC	2 cut(s)	295, 614
Eco53kI	GAGCTC	1 cut(s)	216
Eco57I	CTGAAG	2 cut(s)	987, 1227
Eco88I	CYCGRG	2 cut(s)	695, 1128
EcoICRI	GAGCTC	1 cut(s)	216
EcoO109I	RGGNCCY	2 cut(s)	295, 1637
EcoRII	CCWGG	2 cut(s)	790, 987
EcoT38I	GRGCYC	2 cut(s)	218, 1641
Esp3I	CGTCTC	1 cut(s)	1357
FaeI	CATG	7 cut(s)	452, 490, 886, 1235, 1250, 1399, 1678
FaqI	GGGAC	2 cut(s)	254, 1461
FatI	CATG	7 cut(s)	448, 486, 882, 1231, 1246, 1395, 1674
FauI	CCCGC	2 cut(s)	261, 1429
Fnu4HI	GCNGC	9 cut(s)	171, 222, 338, 552, 1278, 1584, 1587, 1590, 1710
FokI	GGATG	1 cut(s)	362
FriOI	GRGCYC	2 cut(s)	218, 1641
Fsp4HI	GCNGC	9 cut(s)	171, 222, 338, 552, 1278, 1584, 1587, 1590, 1710
FspBI	CTAG	7 cut(s)	423, 620, 642, 837, 857, 1538, 1605
GlaI	GCGC	3 cut(s)	73, 79, 526
GluI	GCNGC	9 cut(s)	171, 222, 338, 552, 1278, 1584, 1587, 1590, 1710
HaeII	RGCGCY	2 cut(s)	75, 81
HaeIII	GGCC	3 cut(s)	236, 635, 1639
HapII	CCGG	7 cut(s)	93, 208, 233, 311, 383, 1414, 1688
HgaI	GACGC	2 cut(s)	110, 269
HhaI	GCGC	3 cut(s)	74, 80, 527
Hin1I	GRCGYC	2 cut(s)	261, 1350
Hin1II	CATG	7 cut(s)	452, 490, 886, 1235, 1250, 1399, 1678
Hin6I	GCGC	3 cut(s)	72, 78, 525
HinP1I	GCGC	3 cut(s)	72, 78, 525
HindIII	AAGCTT	1 cut(s)	1209
HinfI	GANTC	5 cut(s)	368, 395, 698, 798, 1121
HpaII	CCGG	7 cut(s)	93, 208, 233, 311, 383, 1414, 1688
HphI	GGTGA	3 cut(s)	11, 620, 922
Hpy166II	GTNNAC	2 cut(s)	1253, 1411
Hpy188I	TCNGA	8 cut(s)	111, 289, 302, 754, 928, 1059, 1389, 1684
Hpy188III	TCNNGA	9 cut(s)	93, 365, 695, 837, 857, 1076, 1128, 1356, 1475
Hpy8I	GTNNAC	2 cut(s)	1253, 1411
Hpy99I	CGWCG	4 cut(s)	104, 263, 269, 289
HpyAV	CCTTC	8 cut(s)	427, 501, 971, 1012, 1063, 1416, 1471, 1560
HpyCH4III	ACNGT	1 cut(s)	1082
HpyCH4IV	ACGT	3 cut(s)	1255, 1350, 1368
HpyF10VI	GCNNNNNNNGC	6 cut(s)	242, 557, 1139, 1217, 1589, 1718
HpyF3I	CTNAG	7 cut(s)	23, 273, 500, 741, 753, 1056, 1354
HpySE526I	ACGT	3 cut(s)	1255, 1350, 1368
Hsp92I	GRCGYC	2 cut(s)	261, 1350
Hsp92II	CATG	7 cut(s)	452, 490, 886, 1235, 1250, 1399, 1678
HspAI	GCGC	3 cut(s)	72, 78, 525
Kpn2I	TCCGGA	1 cut(s)	92
KroI	GCCGGC	1 cut(s)	232
KroNI	GCCGGC	1 cut(s)	234
Kzo9I	GATC	8 cut(s)	106, 749, 805, 1093, 1381, 1439, 1471, 1523
LguI	GCTCTTC	3 cut(s)	1248, 1385, 1426
LmnI	GCTCC	5 cut(s)	111, 209, 213, 221, 379
Lsp1109I	GCAGC	7 cut(s)	157, 538, 1264, 1573, 1576, 1595, 1721
LweI	GCATC	3 cut(s)	903, 943, 1343
MaeI	CTAG	7 cut(s)	423, 620, 642, 837, 857, 1538, 1605
MaeII	ACGT	3 cut(s)	1255, 1350, 1368
MaeIII	GTNAC	1 cut(s)	1051
MalI	GATC	8 cut(s)	108, 751, 807, 1095, 1383, 1441, 1473, 1525
MbiI	CCGCTC	2 cut(s)	224, 1436
MboI	GATC	8 cut(s)	106, 749, 805, 1093, 1381, 1439, 1471, 1523
MfeI	CAATTG	1 cut(s)	390
MflI	RGATCY	3 cut(s)	749, 1381, 1523
MhlI	GDGCHC	2 cut(s)	218, 1641
MluCI	AATT	6 cut(s)	343, 390, 471, 840, 1289, 1375
MlyI	GAGTC	4 cut(s)	362, 404, 707, 1130
MmeI	TCCRAC	3 cut(s)	159, 171, 267
MroI	TCCGGA	1 cut(s)	92
MroNI	GCCGGC	1 cut(s)	232
MseI	TTAA	3 cut(s)	342, 771, 1160
MslI	CAYNNNNRTG	3 cut(s)	198, 566, 830
MspA1I	CMGCKG	2 cut(s)	170, 1586
MspI	CCGG	7 cut(s)	93, 208, 233, 311, 383, 1414, 1688
MspR9I	CCNGG	4 cut(s)	208, 383, 792, 989
MunI	CAATTG	1 cut(s)	390
Mva1269I	GAATGC	2 cut(s)	879, 1537
MvaI	CCWGG	2 cut(s)	792, 989
MwoI	GCNNNNNNNGC	6 cut(s)	242, 557, 1139, 1217, 1589, 1718
NaeI	GCCGGC	1 cut(s)	234
NciI	CCSGG	2 cut(s)	208, 383
NdeII	GATC	8 cut(s)	106, 749, 805, 1093, 1381, 1439, 1471, 1523
NgoMIV	GCCGGC	1 cut(s)	232
NlaIII	CATG	7 cut(s)	452, 490, 886, 1235, 1250, 1399, 1678
NspI	RCATGY	1 cut(s)	1235
OliI	CACNNNNGTG	1 cut(s)	830
PaeI	GCATGC	1 cut(s)	1235
PaeR7I	CTCGAG	2 cut(s)	695, 1128
PciSI	GCTCTTC	3 cut(s)	1248, 1385, 1426
PcsI	WCGNNNNNNNCGW	1 cut(s)	298
PctI	GAATGC	2 cut(s)	879, 1537
PdiI	GCCGGC	1 cut(s)	234
PfeI	GAWTC	1 cut(s)	798
PflFI	GACNNNGTC	1 cut(s)	263
PfoI	TCCNGGA	1 cut(s)	790
PkrI	GCNGC	9 cut(s)	172, 223, 339, 553, 1279, 1585, 1588, 1591, 1711
PleI	GAGTC	4 cut(s)	362, 403, 706, 1129
PpsI	GAGTC	4 cut(s)	362, 403, 706, 1129
PpuMI	RGGWCCY	1 cut(s)	295
Psp124BI	GAGCTC	1 cut(s)	218
Psp5II	RGGWCCY	1 cut(s)	295
Psp6I	CCWGG	2 cut(s)	790, 987
PspGI	CCWGG	2 cut(s)	790, 987
PspOMI	GGGCCC	1 cut(s)	1637
PspPI	GGNCC	5 cut(s)	295, 614, 634, 1637, 1638
PspPPI	RGGWCCY	1 cut(s)	295
PstNI	CAGNNNCTG	1 cut(s)	1589
PsuI	RGATCY	3 cut(s)	749, 1381, 1523
PsyI	GACNNNGTC	1 cut(s)	263
PvuII	CAGCTG	1 cut(s)	1586
RsaI	GTAC	3 cut(s)	630, 1258, 1410
RsaNI	GTAC	3 cut(s)	629, 1257, 1409
RseI	CAYNNNNRTG	3 cut(s)	198, 566, 830
SacI	GAGCTC	1 cut(s)	218
SapI	GCTCTTC	3 cut(s)	1248, 1385, 1426
SaqAI	TTAA	3 cut(s)	342, 771, 1160
SatI	GCNGC	9 cut(s)	171, 222, 338, 552, 1278, 1584, 1587, 1590, 1710
Sau3AI	GATC	8 cut(s)	106, 749, 805, 1093, 1381, 1439, 1471, 1523
Sau96I	GGNCC	5 cut(s)	295, 614, 634, 1637, 1638
SchI	GAGTC	4 cut(s)	362, 404, 707, 1130
ScrFI	CCNGG	4 cut(s)	208, 383, 792, 989
SduI	GDGCHC	2 cut(s)	218, 1641
SfaNI	GCATC	3 cut(s)	903, 943, 1343
SfcI	CTRYAG	1 cut(s)	1041
Sfr274I	CTCGAG	2 cut(s)	695, 1128
SinI	GGWCC	2 cut(s)	295, 614
SlaI	CTCGAG	2 cut(s)	695, 1128
SmiMI	CAYNNNNRTG	3 cut(s)	198, 566, 830
SmlI	CTYRAG	3 cut(s)	480, 695, 1128
SmoI	CTYRAG	3 cut(s)	480, 695, 1128
SphI	GCATGC	1 cut(s)	1235
Sse9I	AATT	6 cut(s)	343, 390, 471, 840, 1289, 1375
SsiI	CCGC	8 cut(s)	66, 168, 219, 222, 254, 337, 916, 1436
SspI	AATATT	2 cut(s)	775, 995
SspMI	CTAG	7 cut(s)	423, 620, 642, 837, 857, 1538, 1605
SstI	GAGCTC	1 cut(s)	218
StyD4I	CCNGG	4 cut(s)	206, 381, 790, 987
TaaI	ACNGT	1 cut(s)	1082
TaiI	ACGT	3 cut(s)	1258, 1353, 1371
TaqI	TCGA	3 cut(s)	696, 760, 1129
TasI	AATT	6 cut(s)	343, 390, 471, 840, 1289, 1375
TatI	WGTACW	1 cut(s)	1408
TauI	GCSGC	2 cut(s)	224, 340
TfiI	GAWTC	1 cut(s)	798
Tru1I	TTAA	3 cut(s)	342, 771, 1160
Tru9I	TTAA	3 cut(s)	342, 771, 1160
TscAI	CASTG	5 cut(s)	281, 508, 1014, 1087, 1474
TseI	GCWGC	7 cut(s)	170, 551, 1277, 1583, 1586, 1589, 1709
TspDTI	ATGAA	8 cut(s)	165, 320, 437, 446, 790, 876, 1235, 1663
TspRI	CASTG	5 cut(s)	281, 508, 1014, 1087, 1474
Tth111I	GACNNNGTC	1 cut(s)	263
VpaK11BI	GGWCC	2 cut(s)	295, 614
XapI	RAATTY	2 cut(s)	840, 1289
XbaI	TCTAGA	2 cut(s)	836, 856
XceI	RCATGY	1 cut(s)	1235
XhoI	CTCGAG	2 cut(s)	695, 1128
XspI	CTAG	7 cut(s)	423, 620, 642, 837, 857, 1538, 1605
ZraI	GACGTC	1 cut(s)	1351

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.