Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0007364

Physical Location & Seq

Genomic Coordinates Forward (+)

4046243 .. 4050832

Gene Structure 4590 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1050900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1452 bp

ATGGACCTCCTCCTCCTGGAAAAGACCCTTCTGGGTCTCTTCGTCGCCGTGATCGTCGCCATCGCCATTTCAAAACTCCGCGGCAAGAAATTCAAGCTCCCGCCTGGTCCCATACCCGTACCCGTTTTCGGAAACTGGCTCCAGGTCGGCGACGACCTCAACCACCGTAATCTCACCGACCTCGCCAAGAAATTCGGCGACTGCTTCCTCCTCCGCATGGGGCAGCGCAACCTCGTCGTCGTCTCCTCGCCGGAGCTCGCCAAGGAGGACATGGTGTTCACCGTCTACGGCGAGCACTGGCGTAAAATGCGGCGGATCATGACTGTCCCCTTCTTCACCAACAAGGTGGTCCAGCAGTACCGCCACGGATGGGAATCTGAGGCGGCGGCGGTCGTCGAGGACGTGAAAAAGCACCCTGAGGCGGCGAAACAGTGGGATGGTGCTGAGGAGGCGGCTGCAGCTGATGATGTACAACAACATGTACAGAATCATGTTCGATCGGCGATTCGAAAGCGAGGAGGATCCCCTGTTCGTGAAGCTCTGAAGCTCAAGGGATTGAACGGGGAGAGGAGCCGATTGGCCCAGAGCTTCGACTACTACAACTACGGCGATTTTATCCCCATTTTAAGGCCCTTTTTGAGAGGGTATTTGAAGATCTGCAAGGAGGTGAAGGAGAAAGAGGATTCAGCTGTGTTTAAGAGCTATTTCGTTGACGAGAGGAAGAAACTTTCAAGCACACGGGCTCCAACAAATGAGGGGTTGAAGTGCGCCATTGACCACATCCTCGACGCGCAGCAGAAGGGAGAAATCAACGAGGACAATGTTCTCTACATCGTCGAGAACATCAATGTTGCTGCTATTGAAACCACATTGTGGTCGATCGAGTGGGGAATTGCCGAGCTAGTGAACCATCCTGAAATCCAGAAGAAGTTGAGGGAGGAACTTGACGCTGTCCTCGGTCGCGGAGTTCAGATCACAGAGCCTGATGTTCAGAAACTTCCCTACCTGCAAGCTGTGATCAAGGAGACTCTACGACTTCGCATGGCAATCCCACTCCTTGTTCCCCATATGAACCTCCAGGACGCCAAGCTTGGCGGGTTTGACATCCCGGCGGAGAGCAAGATTTTGGTGAATGCTTGGTGGTTGGCAAACAACCCTGCCCTGTGGAATAAACCGGAAGAGTTTAGGCCCGAGAGGTTTTTGGAGGAGGAGTCGAAGGTGGAGGCTAACGGGAACGACTTTAGGTATCTCCCGTTTGGTGTTGGGAGGAGAAGTTGTCCCGGGATTATCCTGGCCCTGCCAATCCTAGGCATTACAATTGGACGTTTAGTCCAGAACTTCGAGCTTCTGCCTCCGCCGGGACAGTCCAAGCTCGACACCTCGGAGAAAGGTGGGCAGTTCAGCCTGCACATCCTGAAGCATTCCACCATTGTGATGAAGTCGAGGGCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004497 GO:0005575 GO:0006725 GO:0008150 GO:0008152 GO:0009058 GO:0009698 GO:0009808 GO:0009987 GO:0016020 GO:0016491 GO:0016705 GO:0016709 GO:0016710 GO:0019748 GO:0044237 GO:0044550 GO:0055114 GO:0071704 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko00130 ko00360 ko00940 ko00941 ko00945 ko01100 ko01110 ko01220 map00130 map00360 map00940 map00941 map00945 map01100 map01110 map01220

Pfam Domains

Protein Families

p450

Protein Analysis

484

Amino Acids

54.65

Weight (kDa)

8.76

Isoelectric Point (pI)

47.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
p450	PF00067	34 - 92	3e-11	Cytochrome P450
p450	PF00067	90 - 475	2.3e-83	Cytochrome P450

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007364)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30490
fragaria_vesca	FvH4_3g40570
malus_domestica	MD00G1221400.v1.1 MD03G1050900.v1.1 MD03G1051000.v1.1 MD03G1051100.v1.1 MD03G1051300.v1.1 MD11G1052900.v1.1
prunus_persica	Prupe.6G040400_v2.0.a1
pyrus_communis	pycom03g04020 pycom03g04030 pycom03g04050 pycom03g04060
rosa_chinensis	RchiOBHm_Chr5g0073351
rosa_laevigata	RLG00000036379
rosa_multiflora	Rmu_sc0001997.1_g000001 Rmu_sc0005684.1_g000008 Rmu_sc0006213.1_g000002
rosa_roxburghii	Rroxscaffold_1G00007890
rosa_rugosa	Rorug05G0422900
rosa_samantha	Rh5AG479500 Rh5BG500700 Rh5CG523800 Rh5DG512500
rosa_wichuraiana	Rw5G044580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1014
AccB7I	CCANNNNNTGG	1 cut(s)	873
AccI	GTMKAC	1 cut(s)	285
AccII	CGCG	3 cut(s)	81, 791, 963
AclWI	GGATC	3 cut(s)	323, 516, 529
AcsI	RAATTY	2 cut(s)	89, 191
AcuI	CTGAAG	2 cut(s)	563, 1436
AcyI	GRCGYC	1 cut(s)	1083
AdeI	CACNNNGTG	1 cut(s)	873
AfaI	GTAC	4 cut(s)	120, 359, 471, 483
AfiI	CCNNNNNNNGG	9 cut(s)	16, 128, 217, 370, 421, 627, 873, 1307, 1358
AflIII	ACRYGT	1 cut(s)	478
AgsI	TTSAA	7 cut(s)	72, 94, 559, 652, 732, 763, 863
AhdI	GACNNNNNGTC	1 cut(s)	1328
AjiI	CACGTC	1 cut(s)	403
AjnI	CCWGG	5 cut(s)	15, 103, 141, 1077, 1290
AleI	CACNNNNGTG	1 cut(s)	1430
AloI	GAACNNNNNNTCC	4 cut(s)	260, 292, 513, 545
Alw21I	GWGCWC	2 cut(s)	258, 297
Alw26I	GTCTC	3 cut(s)	41, 247, 1019
AlwI	GGATC	3 cut(s)	323, 516, 529
AlwNI	CAGNNNCTG	1 cut(s)	983
Ama87I	CYCGRG	2 cut(s)	1190, 1280
AoxI	GGCC	4 cut(s)	579, 629, 1187, 1293
ApeKI	GCWGC	5 cut(s)	223, 455, 458, 793, 854
ApoI	RAATTY	2 cut(s)	89, 191
AspA2I	CCTAGG	1 cut(s)	1306
AspLEI	GCGC	3 cut(s)	228, 770, 793
AspS9I	GGNCC	7 cut(s)	4, 107, 349, 580, 630, 1188, 1294
AsuC2I	CCSGG	4 cut(s)	1109, 1281, 1282, 1359
AsuHPI	GGTGA	5 cut(s)	166, 271, 328, 679, 1141
AsuII	TTCGAA	1 cut(s)	508
AvaI	CYCGRG	2 cut(s)	1190, 1280
AvaII	GGWCC	3 cut(s)	4, 107, 349
AvrII	CCTAGG	1 cut(s)	1306
AxyI	CCTNAGG	1 cut(s)	417
BamHI	GGATCC	1 cut(s)	521
BanII	GRGCYC	2 cut(s)	258, 745
Bbv12I	GWGCWC	2 cut(s)	258, 297
BbvCI	CCTCAGC	1 cut(s)	444
BbvI	GCAGC	5 cut(s)	235, 442, 470, 805, 841
BccI	CCATC	4 cut(s)	68, 363, 431, 918
BceAI	ACGGC	3 cut(s)	32, 304, 622
BcgI	CGANNNNNNTGC	2 cut(s)	217, 251
BciT130I	CCWGG	5 cut(s)	17, 105, 143, 1079, 1292
BclI	TGATCA	1 cut(s)	1017
BcnI	CCSGG	4 cut(s)	1109, 1281, 1282, 1359
BcoDI	GTCTC	3 cut(s)	41, 247, 1019
BfaI	CTAG	2 cut(s)	902, 1307
BfmI	CTRYAG	1 cut(s)	456
BfuAI	ACCTGC	1 cut(s)	1014
BglII	AGATCT	1 cut(s)	654
BlnI	CCTAGG	1 cut(s)	1306
Bme1390I	CCNGG	9 cut(s)	17, 105, 143, 1079, 1109, 1281, 1282, 1292, 1359
Bme18I	GGWCC	3 cut(s)	4, 107, 349
BmeRI	GACNNNNNGTC	1 cut(s)	1328
BmeT110I	CYCGRG	2 cut(s)	1190, 1280
BmgBI	CACGTC	1 cut(s)	403
BmgT120I	GGNCC	7 cut(s)	4, 107, 349, 580, 630, 1188, 1294
BmiI	GGNNCC	5 cut(s)	109, 140, 523, 572, 744
BmrFI	CCNGG	9 cut(s)	17, 105, 143, 1079, 1109, 1281, 1282, 1292, 1359
BpmI	CTGGAG	2 cut(s)	125, 1061
Bpu10I	CCTNAGC	1 cut(s)	444
Bpu14I	TTCGAA	1 cut(s)	508
BpuEI	CTTGAG	1 cut(s)	533
BpuMI	CCSGG	4 cut(s)	1109, 1281, 1282, 1359
BsaBI	GATNNNNATC	1 cut(s)	373
BsaHI	GRCGYC	1 cut(s)	1083
BsaI	GGTCTC	1 cut(s)	41
BsaJI	CCNNGG	7 cut(s)	79, 261, 364, 955, 1280, 1306, 1380
BsaWI	WCCGGW	1 cut(s)	1174
BsaXI	ACNNNNNCTCC	8 cut(s)	727, 757, 1196, 1226, 1258, 1261, 1288, 1291
Bsc4I	CCNNNNNNNGG	9 cut(s)	16, 128, 217, 370, 421, 627, 873, 1307, 1358
Bse1I	ACTGG	2 cut(s)	140, 302
Bse21I	CCTNAGG	1 cut(s)	417
Bse8I	GATNNNNATC	1 cut(s)	373
BseBI	CCWGG	5 cut(s)	17, 105, 143, 1079, 1292
BseDI	CCNNGG	7 cut(s)	79, 261, 364, 955, 1280, 1306, 1380
BseGI	GGATG	6 cut(s)	374, 442, 780, 910, 1104, 1410
BseJI	GATNNNNATC	1 cut(s)	373
BseLI	CCNNNNNNNGG	9 cut(s)	16, 128, 217, 370, 421, 627, 873, 1307, 1358
BseMII	CTCAG	3 cut(s)	369, 408, 435
BseNI	ACTGG	2 cut(s)	140, 302
BseRI	GAGGAG	8 cut(s)	200, 235, 461, 531, 583, 1220, 1223, 1282
BseXI	GCAGC	5 cut(s)	235, 442, 470, 805, 841
BsgI	GTGCAG	1 cut(s)	1391
Bsh1236I	CGCG	3 cut(s)	81, 791, 963
Bsh1285I	CGRYCG	4 cut(s)	393, 500, 882, 961
BshFI	GGCC	4 cut(s)	581, 631, 1189, 1295
BsiEI	CGRYCG	4 cut(s)	393, 500, 882, 961
BsiHKAI	GWGCWC	2 cut(s)	258, 297
BsiHKCI	CYCGRG	2 cut(s)	1190, 1280
BsiSI	CCGG	5 cut(s)	251, 1109, 1175, 1281, 1358
BslFI	GGGAC	4 cut(s)	93, 311, 1263, 1374
BslI	CCNNNNNNNGG	9 cut(s)	16, 128, 217, 370, 421, 627, 873, 1307, 1358
BsmAI	GTCTC	3 cut(s)	41, 247, 1019
BsmBI	CGTCTC	1 cut(s)	247
BsmFI	GGGAC	4 cut(s)	93, 311, 1263, 1374
BsmI	GAATGC	2 cut(s)	1138, 1420
BsnI	GGCC	4 cut(s)	581, 631, 1189, 1295
Bso31I	GGTCTC	1 cut(s)	41
BsoBI	CYCGRG	2 cut(s)	1190, 1280
Bsp119I	TTCGAA	1 cut(s)	508
Bsp1286I	GDGCHC	3 cut(s)	258, 297, 745
Bsp1407I	TGTACA	2 cut(s)	469, 481
Bsp143I	GATC	8 cut(s)	51, 315, 497, 521, 654, 879, 972, 1017
BspANI	GGCC	4 cut(s)	581, 631, 1189, 1295
BspCNI	CTCAG	3 cut(s)	370, 409, 436
BspFNI	CGCG	3 cut(s)	81, 791, 963
BspHI	TCATGA	1 cut(s)	318
BspLI	GGNNCC	5 cut(s)	109, 140, 523, 572, 744
BspMAI	CTGCAG	1 cut(s)	460
BspMI	ACCTGC	1 cut(s)	1014
BspPI	GGATC	3 cut(s)	323, 516, 529
BspT104I	TTCGAA	1 cut(s)	508
BspTNI	GGTCTC	1 cut(s)	41
BsrGI	TGTACA	2 cut(s)	469, 481
BsrI	ACTGG	2 cut(s)	140, 302
BssECI	CCNNGG	7 cut(s)	79, 261, 364, 955, 1280, 1306, 1380
BssMI	GATC	8 cut(s)	51, 315, 497, 521, 654, 879, 972, 1017
BssNI	GRCGYC	1 cut(s)	1083
BssT1I	CCWWGG	2 cut(s)	261, 1306
Bst2UI	CCWGG	5 cut(s)	17, 105, 143, 1079, 1292
Bst4CI	ACNGT	5 cut(s)	167, 283, 325, 432, 1365
Bst6I	CTCTTC	2 cut(s)	44, 1173
BstACI	GRCGYC	1 cut(s)	1083
BstAUI	TGTACA	2 cut(s)	469, 481
BstBI	TTCGAA	1 cut(s)	508
BstC8I	GCNNGC	4 cut(s)	258, 293, 1011, 1406
BstDEI	CTNAG	3 cut(s)	378, 417, 444
BstDSI	CCRYGG	2 cut(s)	79, 364
BstF5I	GGATG	6 cut(s)	374, 442, 780, 910, 1104, 1410
BstFNI	CGCG	3 cut(s)	81, 791, 963
BstHHI	GCGC	3 cut(s)	228, 770, 793
BstKTI	GATC	8 cut(s)	54, 318, 500, 524, 657, 882, 975, 1020
BstMAI	GTCTC	3 cut(s)	41, 247, 1019
BstMBI	GATC	8 cut(s)	51, 315, 497, 521, 654, 879, 972, 1017
BstMCI	CGRYCG	4 cut(s)	393, 500, 882, 961
BstMWI	GCNNNNNNNGC	3 cut(s)	307, 449, 458
BstNI	CCWGG	5 cut(s)	17, 105, 143, 1079, 1292
BstNSI	RCATGY	1 cut(s)	482
BstSCI	CCNGG	9 cut(s)	15, 103, 141, 1077, 1107, 1279, 1280, 1290, 1357
BstSFI	CTRYAG	1 cut(s)	456
BstUI	CGCG	3 cut(s)	81, 791, 963
BstV1I	GCAGC	5 cut(s)	235, 442, 470, 805, 841
BstX2I	RGATCY	2 cut(s)	521, 654
BstXI	CCANNNNNNTGG	1 cut(s)	346
BstYI	RGATCY	2 cut(s)	521, 654
Bsu36I	CCTNAGG	1 cut(s)	417
BsuRI	GGCC	4 cut(s)	581, 631, 1189, 1295
BtgI	CCRYGG	2 cut(s)	79, 364
BtgZI	GCGATG	1 cut(s)	46
BtrI	CACGTC	1 cut(s)	403
BtsCI	GGATG	6 cut(s)	374, 442, 780, 910, 1104, 1410
BtsIMutI	CAGTG	2 cut(s)	295, 437
BveI	ACCTGC	1 cut(s)	1014
Cac8I	GCNNGC	4 cut(s)	258, 293, 1011, 1406
CaiI	CAGNNNCTG	1 cut(s)	983
CciI	TCATGA	1 cut(s)	318
CfoI	GCGC	3 cut(s)	228, 770, 793
Cfr13I	GGNCC	7 cut(s)	4, 107, 349, 580, 630, 1188, 1294
Cfr42I	CCGCGG	1 cut(s)	82
Cfr9I	CCCGGG	1 cut(s)	1280
CseI	GACGC	3 cut(s)	797, 956, 1091
Csp6I	GTAC	4 cut(s)	119, 358, 470, 482
CviAII	CATG	6 cut(s)	217, 271, 319, 479, 491, 1042
CviQI	GTAC	4 cut(s)	119, 358, 470, 482
DdeI	CTNAG	3 cut(s)	378, 417, 444
DpnI	GATC	8 cut(s)	53, 317, 499, 523, 656, 881, 974, 1019
DpnII	GATC	8 cut(s)	51, 315, 497, 521, 654, 879, 972, 1017
DraIII	CACNNNGTG	1 cut(s)	873
DriI	GACNNNNNGTC	1 cut(s)	1328
Eam1104I	CTCTTC	2 cut(s)	44, 1173
Eam1105I	GACNNNNNGTC	1 cut(s)	1328
EarI	CTCTTC	2 cut(s)	44, 1173
EciI	GGCGGA	3 cut(s)	328, 1127, 1344
Ecl136II	GAGCTC	1 cut(s)	256
Eco130I	CCWWGG	2 cut(s)	261, 1306
Eco24I	GRGCYC	2 cut(s)	258, 745
Eco31I	GGTCTC	1 cut(s)	41
Eco47I	GGWCC	3 cut(s)	4, 107, 349
Eco53kI	GAGCTC	1 cut(s)	256
Eco57I	CTGAAG	2 cut(s)	563, 1436
Eco81I	CCTNAGG	1 cut(s)	417
Eco88I	CYCGRG	2 cut(s)	1190, 1280
EcoICRI	GAGCTC	1 cut(s)	256
EcoO109I	RGGNCCY	1 cut(s)	630
EcoRII	CCWGG	5 cut(s)	15, 103, 141, 1077, 1290
EcoT14I	CCWWGG	2 cut(s)	261, 1306
EcoT38I	GRGCYC	2 cut(s)	258, 745
ErhI	CCWWGG	2 cut(s)	261, 1306
Esp3I	CGTCTC	1 cut(s)	247
FaeI	CATG	6 cut(s)	220, 274, 322, 482, 494, 1045
FaiI	YATR	9 cut(s)	113, 218, 272, 320, 480, 492, 1043, 1068, 1070
FaqI	GGGAC	4 cut(s)	93, 311, 1263, 1374
FatI	CATG	6 cut(s)	216, 270, 318, 478, 490, 1041
FauI	CCCGC	2 cut(s)	108, 1088
FauNDI	CATATG	1 cut(s)	1068
FbaI	TGATCA	1 cut(s)	1017
FblI	GTMKAC	1 cut(s)	285
FokI	GGATG	6 cut(s)	381, 449, 767, 897, 1091, 1397
FriOI	GRGCYC	2 cut(s)	258, 745
FspBI	CTAG	2 cut(s)	902, 1307
GlaI	GCGC	3 cut(s)	227, 769, 792
GsuI	CTGGAG	2 cut(s)	125, 1061
HaeIII	GGCC	4 cut(s)	581, 631, 1189, 1295
HapII	CCGG	5 cut(s)	251, 1109, 1175, 1281, 1358
HgaI	GACGC	3 cut(s)	797, 956, 1091
HhaI	GCGC	3 cut(s)	228, 770, 793
Hin1I	GRCGYC	1 cut(s)	1083
Hin1II	CATG	6 cut(s)	220, 274, 322, 482, 494, 1045
Hin6I	GCGC	3 cut(s)	226, 768, 791
HinP1I	GCGC	3 cut(s)	226, 768, 791
HincII	GTYRAC	1 cut(s)	712
HindII	GTYRAC	1 cut(s)	712
HindIII	AAGCTT	1 cut(s)	1088
HinfI	GANTC	6 cut(s)	374, 487, 505, 683, 1027, 1211
HpaII	CCGG	5 cut(s)	251, 1109, 1175, 1281, 1358
HphI	GGTGA	5 cut(s)	166, 271, 328, 679, 1141
Hpy166II	GTNNAC	4 cut(s)	279, 286, 712, 907
Hpy188I	TCNGA	6 cut(s)	131, 379, 543, 972, 993, 1384
Hpy188III	TCNNGA	7 cut(s)	319, 533, 838, 914, 922, 1333, 1414
Hpy8I	GTNNAC	4 cut(s)	279, 286, 712, 907
Hpy99I	CGWCG	8 cut(s)	47, 59, 155, 239, 242, 398, 791, 839
HpyAV	CCTTC	5 cut(s)	38, 340, 664, 793, 1210
HpyCH4III	ACNGT	5 cut(s)	167, 283, 325, 432, 1365
HpyCH4IV	ACGT	2 cut(s)	402, 1324
HpyCH4V	TGCA	4 cut(s)	458, 660, 1009, 1408
HpyF10VI	GCNNNNNNNGC	3 cut(s)	307, 449, 458
HpyF3I	CTNAG	3 cut(s)	378, 417, 444
HpySE526I	ACGT	2 cut(s)	402, 1324
Hsp92I	GRCGYC	1 cut(s)	1083
Hsp92II	CATG	6 cut(s)	220, 274, 322, 482, 494, 1045
HspAI	GCGC	3 cut(s)	226, 768, 791
Ksp22I	TGATCA	1 cut(s)	1017
KspI	CCGCGG	1 cut(s)	82
Kzo9I	GATC	8 cut(s)	51, 315, 497, 521, 654, 879, 972, 1017
LmnI	GCTCC	5 cut(s)	102, 144, 253, 570, 748
Lsp1109I	GCAGC	5 cut(s)	235, 442, 470, 805, 841
MaeI	CTAG	2 cut(s)	902, 1307
MaeII	ACGT	2 cut(s)	402, 1324
MalI	GATC	8 cut(s)	53, 317, 499, 523, 656, 881, 974, 1019
MboI	GATC	8 cut(s)	51, 315, 497, 521, 654, 879, 972, 1017
MboII	GAAGA	6 cut(s)	31, 325, 664, 733, 937, 1190
MfeI	CAATTG	1 cut(s)	1317
MflI	RGATCY	2 cut(s)	521, 654
MhlI	GDGCHC	3 cut(s)	258, 297, 745
MluCI	AATT	4 cut(s)	89, 191, 891, 1317
MlyI	GAGTC	2 cut(s)	1021, 1220
MmeI	TCCRAC	1 cut(s)	770
MseI	TTAA	2 cut(s)	626, 696
MslI	CAYNNNNRTG	2 cut(s)	1430, 1433
MspA1I	CMGCKG	3 cut(s)	81, 461, 689
MspI	CCGG	5 cut(s)	251, 1109, 1175, 1281, 1358
MspR9I	CCNGG	9 cut(s)	17, 105, 143, 1079, 1109, 1281, 1282, 1292, 1359
MunI	CAATTG	1 cut(s)	1317
Mva1269I	GAATGC	2 cut(s)	1138, 1420
MvaI	CCWGG	5 cut(s)	17, 105, 143, 1079, 1292
MvnI	CGCG	3 cut(s)	81, 791, 963
MwoI	GCNNNNNNNGC	3 cut(s)	307, 449, 458
NciI	CCSGG	4 cut(s)	1109, 1281, 1282, 1359
NdeI	CATATG	1 cut(s)	1068
NdeII	GATC	8 cut(s)	51, 315, 497, 521, 654, 879, 972, 1017
NlaIII	CATG	6 cut(s)	220, 274, 322, 482, 494, 1045
NlaIV	GGNNCC	5 cut(s)	109, 140, 523, 572, 744
NmeAIII	GCCGAG	1 cut(s)	922
NspI	RCATGY	1 cut(s)	482
NspV	TTCGAA	1 cut(s)	508
OliI	CACNNNNGTG	1 cut(s)	1430
PagI	TCATGA	1 cut(s)	318
PciI	ACATGT	1 cut(s)	478
PcsI	WCGNNNNNNNCGW	2 cut(s)	51, 399
PctI	GAATGC	2 cut(s)	1138, 1420
PfeI	GAWTC	4 cut(s)	374, 487, 505, 683
PflFI	GACNNNGTC	1 cut(s)	950
PflMI	CCANNNNNTGG	1 cut(s)	873
PfoI	TCCNGGA	2 cut(s)	15, 1077
Ple19I	CGATCG	2 cut(s)	500, 882
PleI	GAGTC	2 cut(s)	1021, 1219
PpsI	GAGTC	2 cut(s)	1021, 1219
PscI	ACATGT	1 cut(s)	478
Psp124BI	GAGCTC	1 cut(s)	258
Psp6I	CCWGG	5 cut(s)	15, 103, 141, 1077, 1290
PspGI	CCWGG	5 cut(s)	15, 103, 141, 1077, 1290
PspN4I	GGNNCC	5 cut(s)	109, 140, 523, 572, 744
PspPI	GGNCC	7 cut(s)	4, 107, 349, 580, 630, 1188, 1294
PstI	CTGCAG	1 cut(s)	460
PstNI	CAGNNNCTG	1 cut(s)	983
PsuI	RGATCY	2 cut(s)	521, 654
PsyI	GACNNNGTC	1 cut(s)	950
PvuI	CGATCG	2 cut(s)	500, 882
PvuII	CAGCTG	2 cut(s)	461, 689
RsaI	GTAC	4 cut(s)	120, 359, 471, 483
RsaNI	GTAC	4 cut(s)	119, 358, 470, 482
RseI	CAYNNNNRTG	2 cut(s)	1430, 1433
SacI	GAGCTC	1 cut(s)	258
SacII	CCGCGG	1 cut(s)	82
SaqAI	TTAA	2 cut(s)	626, 696
Sau3AI	GATC	8 cut(s)	51, 315, 497, 521, 654, 879, 972, 1017
Sau96I	GGNCC	7 cut(s)	4, 107, 349, 580, 630, 1188, 1294
SchI	GAGTC	2 cut(s)	1021, 1220
ScrFI	CCNGG	9 cut(s)	17, 105, 143, 1079, 1109, 1281, 1282, 1292, 1359
SduI	GDGCHC	3 cut(s)	258, 297, 745
SfcI	CTRYAG	1 cut(s)	456
Sfr303I	CCGCGG	1 cut(s)	82
SfuI	TTCGAA	1 cut(s)	508
SgrBI	CCGCGG	1 cut(s)	82
SinI	GGWCC	3 cut(s)	4, 107, 349
SmaI	CCCGGG	1 cut(s)	1282
SmiMI	CAYNNNNRTG	2 cut(s)	1430, 1433
SmlI	CTYRAG	1 cut(s)	548
SmoI	CTYRAG	1 cut(s)	548
Sse9I	AATT	4 cut(s)	89, 191, 891, 1317
SspMI	CTAG	2 cut(s)	902, 1307
SstI	GAGCTC	1 cut(s)	258
StyD4I	CCNGG	9 cut(s)	15, 103, 141, 1077, 1107, 1279, 1280, 1290, 1357
StyI	CCWWGG	2 cut(s)	261, 1306
TaaI	ACNGT	5 cut(s)	167, 283, 325, 432, 1365
TaiI	ACGT	2 cut(s)	405, 1327
TaqII	GACCGA	1 cut(s)	947
TasI	AATT	4 cut(s)	89, 191, 891, 1317
TatI	WGTACW	2 cut(s)	469, 481
TauI	GCSGC	6 cut(s)	84, 313, 386, 389, 425, 455
TfiI	GAWTC	4 cut(s)	374, 487, 505, 683
Tru1I	TTAA	2 cut(s)	626, 696
Tru9I	TTAA	2 cut(s)	626, 696
TscAI	CASTG	2 cut(s)	302, 437
TseI	GCWGC	5 cut(s)	223, 455, 458, 793, 854
TspDTI	ATGAA	2 cut(s)	1085, 1451
TspGWI	ACGGA	1 cut(s)	381
TspMI	CCCGGG	1 cut(s)	1280
TspRI	CASTG	2 cut(s)	302, 437
Tth111I	GACNNNGTC	1 cut(s)	950
Van91I	CCANNNNNTGG	1 cut(s)	873
VpaK11BI	GGWCC	3 cut(s)	4, 107, 349
XapI	RAATTY	2 cut(s)	89, 191
XceI	RCATGY	1 cut(s)	482
XcmI	CCANNNNNNNNNTGG	1 cut(s)	268
XmaI	CCCGGG	1 cut(s)	1280
XmaJI	CCTAGG	1 cut(s)	1306
XmiI	GTMKAC	1 cut(s)	285
XspI	CTAG	2 cut(s)	902, 1307

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.