pycom03g04020

Belongs to the cytochrome P450 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0007364
Physical Location & Seq
Forward (+)
3174756 .. 3175214
459 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g04020.1

Sequence Viewer

Length: 459 bp
ATGGATCTCCTCCTCCTGGAAAAGACCCTTCTGGGTCTCTTCGTCGCCGTGATCGTTGCCATCGCCATTTCAAAACTCCGCGGCAAGAAATTCAAGCTCCCGCCGGGTCCCATACCCGTACCTGTTTTTGGAAACTGGCTCCAGGTCGGCGACGACCTCAACCACCGTAATCTCACCGACCTCGCCAAGAAATTCGGCGACTGCTTCCTCCTCCGCATGGGGCAGCGCAACCTCGTCGTCGTCTCCTCGCCGGAGCTCGCCAAGGAGGTGCTCCACACCCAGGGAGTTGAATTCGGGTCGAGGACCCGAAACGTCGTCTTTGATATCTTCACCGGTGAAGGCCAGGACATGGTGTTCACCGTCTACGGCGAGCACTGGCGGAAAATGCGGCTGGATCATGACCGTCCCCTTCTTCACCAACAAGGTGGTTCAGCAGTACCGCCACGGATGGGAATCTGA

Protein Analysis

153

Amino Acids

17.03

Weight (kDa)

9.46

Isoelectric Point (pI)

28.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 34 - 130 3.8e-22 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 349
AccI GTMKAC 1 cut(s) 363
AccII CGCG 1 cut(s) 81
AciI CCGC 7 cut(s) 79, 81, 101, 214, 379, 388, 440
AclWI GGATC 2 cut(s) 12, 402
AcsI RAATTY 3 cut(s) 89, 191, 290
AfaI GTAC 2 cut(s) 120, 438
AfiI CCNNNNNNNGG 6 cut(s) 16, 128, 217, 280, 349, 449
AgeI ACCGGT 1 cut(s) 332
AgsI TTSAA 3 cut(s) 72, 94, 290
AjnI CCWGG 4 cut(s) 15, 141, 279, 342
AloI GAACNNNNNNTCC 2 cut(s) 338, 370
AluBI AGCT 2 cut(s) 97, 256
AluI AGCT 2 cut(s) 97, 256
Alw21I GWGCWC 3 cut(s) 258, 273, 375
Alw26I GTCTC 2 cut(s) 41, 247
AlwI GGATC 2 cut(s) 12, 402
AoxI GGCC 1 cut(s) 340
ApeKI GCWGC 1 cut(s) 223
ApoI RAATTY 3 cut(s) 89, 191, 290
AsiGI ACCGGT 1 cut(s) 332
AspLEI GCGC 1 cut(s) 228
AspS9I GGNCC 2 cut(s) 107, 303
AsuC2I CCSGG 1 cut(s) 105
AsuHPI GGTGA 5 cut(s) 166, 322, 347, 349, 407
AvaII GGWCC 2 cut(s) 107, 303
BanII GRGCYC 1 cut(s) 258
Bbv12I GWGCWC 3 cut(s) 258, 273, 375
BbvI GCAGC 1 cut(s) 235
BccI CCATC 2 cut(s) 68, 442
BceAI ACGGC 2 cut(s) 32, 382
BcgI CGANNNNNNTGC 2 cut(s) 217, 251
BciT130I CCWGG 4 cut(s) 17, 143, 281, 344
BcnI CCSGG 1 cut(s) 105
BcoDI GTCTC 2 cut(s) 41, 247
BisI GCNGC 3 cut(s) 82, 224, 389
BlsI GCNGC 3 cut(s) 83, 225, 390
Bme1390I CCNGG 5 cut(s) 17, 105, 143, 281, 344
Bme18I GGWCC 2 cut(s) 107, 303
BmgT120I GGNCC 2 cut(s) 107, 303
BmiI GGNNCC 4 cut(s) 108, 109, 140, 305
BmrFI CCNGG 5 cut(s) 17, 105, 143, 281, 344
BpmI CTGGAG 1 cut(s) 125
BpuMI CCSGG 1 cut(s) 105
BsaBI GATNNNNATC 1 cut(s) 452
BsaI GGTCTC 1 cut(s) 41
BsaJI CCNNGG 5 cut(s) 79, 261, 279, 280, 443
BsaWI WCCGGW 1 cut(s) 332
Bsc4I CCNNNNNNNGG 6 cut(s) 16, 128, 217, 280, 349, 449
Bse118I RCCGGY 1 cut(s) 332
Bse1I ACTGG 2 cut(s) 140, 380
Bse8I GATNNNNATC 1 cut(s) 452
BseBI CCWGG 4 cut(s) 17, 143, 281, 344
BseDI CCNNGG 5 cut(s) 79, 261, 279, 280, 443
BseGI GGATG 1 cut(s) 453
BseJI GATNNNNATC 1 cut(s) 452
BseLI CCNNNNNNNGG 6 cut(s) 16, 128, 217, 280, 349, 449
BseNI ACTGG 2 cut(s) 140, 380
BseRI GAGGAG 2 cut(s) 200, 235
BseXI GCAGC 1 cut(s) 235
Bsh1236I CGCG 1 cut(s) 81
BshFI GGCC 1 cut(s) 342
BshTI ACCGGT 1 cut(s) 332
BsiHKAI GWGCWC 3 cut(s) 258, 273, 375
BsiSI CCGG 3 cut(s) 104, 251, 333
BslFI GGGAC 2 cut(s) 93, 390
BslI CCNNNNNNNGG 6 cut(s) 16, 128, 217, 280, 349, 449
BsmAI GTCTC 2 cut(s) 41, 247
BsmBI CGTCTC 1 cut(s) 247
BsmFI GGGAC 2 cut(s) 93, 390
BsnI GGCC 1 cut(s) 342
Bso31I GGTCTC 1 cut(s) 41
Bsp1286I GDGCHC 3 cut(s) 258, 273, 375
Bsp143I GATC 3 cut(s) 4, 51, 394
BspACI CCGC 7 cut(s) 79, 81, 101, 214, 379, 388, 440
BspANI GGCC 1 cut(s) 342
BspFNI CGCG 1 cut(s) 81
BspHI TCATGA 1 cut(s) 397
BspLI GGNNCC 4 cut(s) 108, 109, 140, 305
BspPI GGATC 2 cut(s) 12, 402
BspTNI GGTCTC 1 cut(s) 41
BsrFI RCCGGY 1 cut(s) 332
BsrI ACTGG 2 cut(s) 140, 380
BssAI RCCGGY 1 cut(s) 332
BssECI CCNNGG 5 cut(s) 79, 261, 279, 280, 443
BssMI GATC 3 cut(s) 4, 51, 394
BssT1I CCWWGG 1 cut(s) 261
Bst2UI CCWGG 4 cut(s) 17, 143, 281, 344
Bst4CI ACNGT 3 cut(s) 167, 361, 404
Bst6I CTCTTC 1 cut(s) 44
BstC8I GCNNGC 2 cut(s) 258, 371
BstDSI CCRYGG 2 cut(s) 79, 443
BstF5I GGATG 1 cut(s) 453
BstFNI CGCG 1 cut(s) 81
BstHHI GCGC 1 cut(s) 228
BstKTI GATC 3 cut(s) 7, 54, 397
BstMAI GTCTC 2 cut(s) 41, 247
BstMBI GATC 3 cut(s) 4, 51, 394
BstMWI GCNNNNNNNGC 1 cut(s) 385
BstNI CCWGG 4 cut(s) 17, 143, 281, 344
BstSCI CCNGG 5 cut(s) 15, 103, 141, 279, 342
BstUI CGCG 1 cut(s) 81
BstV1I GCAGC 1 cut(s) 235
BstX2I RGATCY 1 cut(s) 4
BstXI CCANNNNNNTGG 1 cut(s) 425
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 1 cut(s) 342
BtgI CCRYGG 2 cut(s) 79, 443
BtgZI GCGATG 1 cut(s) 46
BtsCI GGATG 1 cut(s) 453
BtsIMutI CAGTG 1 cut(s) 373
Cac8I GCNNGC 2 cut(s) 258, 371
CciI TCATGA 1 cut(s) 397
CfoI GCGC 1 cut(s) 228
Cfr10I RCCGGY 1 cut(s) 332
Cfr13I GGNCC 2 cut(s) 107, 303
Cfr42I CCGCGG 1 cut(s) 82
Csp6I GTAC 2 cut(s) 119, 437
CspAI ACCGGT 1 cut(s) 332
CviAII CATG 3 cut(s) 217, 349, 398
CviJI RGCY 5 cut(s) 97, 139, 256, 342, 391
CviKI_1 RGCY 5 cut(s) 97, 139, 256, 342, 391
CviQI GTAC 2 cut(s) 119, 437
DpnI GATC 3 cut(s) 6, 53, 396
DpnII GATC 3 cut(s) 4, 51, 394
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
EciI GGCGGA 1 cut(s) 394
Ecl136II GAGCTC 1 cut(s) 256
Eco130I CCWWGG 1 cut(s) 261
Eco24I GRGCYC 1 cut(s) 258
Eco31I GGTCTC 1 cut(s) 41
Eco32I GATATC 1 cut(s) 325
Eco47I GGWCC 2 cut(s) 107, 303
Eco53kI GAGCTC 1 cut(s) 256
EcoICRI GAGCTC 1 cut(s) 256
EcoO109I RGGNCCY 2 cut(s) 107, 303
EcoRI GAATTC 1 cut(s) 290
EcoRII CCWGG 4 cut(s) 15, 141, 279, 342
EcoRV GATATC 1 cut(s) 325
EcoT14I CCWWGG 1 cut(s) 261
EcoT38I GRGCYC 1 cut(s) 258
ErhI CCWWGG 1 cut(s) 261
Esp3I CGTCTC 1 cut(s) 247
FaeI CATG 3 cut(s) 220, 352, 401
FaiI YATR 4 cut(s) 113, 218, 350, 399
FaqI GGGAC 2 cut(s) 93, 390
FatI CATG 3 cut(s) 216, 348, 397
FauI CCCGC 1 cut(s) 108
FblI GTMKAC 1 cut(s) 363
Fnu4HI GCNGC 3 cut(s) 82, 224, 389
FriOI GRGCYC 1 cut(s) 258
Fsp4HI GCNGC 3 cut(s) 82, 224, 389
GlaI GCGC 1 cut(s) 227
GluI GCNGC 3 cut(s) 82, 224, 389
GsuI CTGGAG 1 cut(s) 125
HaeIII GGCC 1 cut(s) 342
HapII CCGG 3 cut(s) 104, 251, 333
HhaI GCGC 1 cut(s) 228
Hin1II CATG 3 cut(s) 220, 352, 401
Hin6I GCGC 1 cut(s) 226
HinP1I GCGC 1 cut(s) 226
HinfI GANTC 1 cut(s) 453
HpaII CCGG 3 cut(s) 104, 251, 333
HphI GGTGA 5 cut(s) 166, 322, 347, 349, 407
Hpy166II GTNNAC 2 cut(s) 357, 364
Hpy188I TCNGA 1 cut(s) 458
Hpy188III TCNNGA 1 cut(s) 398
Hpy8I GTNNAC 2 cut(s) 357, 364
Hpy99I CGWCG 5 cut(s) 47, 155, 239, 242, 317
HpyAV CCTTC 3 cut(s) 38, 332, 419
HpyCH4III ACNGT 3 cut(s) 167, 361, 404
HpyCH4IV ACGT 1 cut(s) 312
HpyF10VI GCNNNNNNNGC 1 cut(s) 385
HpySE526I ACGT 1 cut(s) 312
Hsp92II CATG 3 cut(s) 220, 352, 401
HspAI GCGC 1 cut(s) 226
KflI GGGWCCC 1 cut(s) 107
KspI CCGCGG 1 cut(s) 82
Kzo9I GATC 3 cut(s) 4, 51, 394
LmnI GCTCC 4 cut(s) 102, 144, 253, 276
Lsp1109I GCAGC 1 cut(s) 235
MaeII ACGT 1 cut(s) 312
MalI GATC 3 cut(s) 6, 53, 396
MboI GATC 3 cut(s) 4, 51, 394
MboII GAAGA 3 cut(s) 31, 319, 404
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 3 cut(s) 258, 273, 375
MluCI AATT 3 cut(s) 89, 191, 290
MspA1I CMGCKG 1 cut(s) 81
MspI CCGG 3 cut(s) 104, 251, 333
MspR9I CCNGG 5 cut(s) 17, 105, 143, 281, 344
MvaI CCWGG 4 cut(s) 17, 143, 281, 344
MvnI CGCG 1 cut(s) 81
MwoI GCNNNNNNNGC 1 cut(s) 385
NciI CCSGG 1 cut(s) 105
NdeII GATC 3 cut(s) 4, 51, 394
NlaIII CATG 3 cut(s) 220, 352, 401
NlaIV GGNNCC 4 cut(s) 108, 109, 140, 305
PagI TCATGA 1 cut(s) 397
PasI CCCWGGG 1 cut(s) 280
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PfeI GAWTC 1 cut(s) 453
PflMI CCANNNNNTGG 1 cut(s) 349
PfoI TCCNGGA 1 cut(s) 15
PinAI ACCGGT 1 cut(s) 332
PkrI GCNGC 3 cut(s) 83, 225, 390
PpuMI RGGWCCY 2 cut(s) 107, 303
Psp124BI GAGCTC 1 cut(s) 258
Psp5II RGGWCCY 2 cut(s) 107, 303
Psp6I CCWGG 4 cut(s) 15, 141, 279, 342
PspGI CCWGG 4 cut(s) 15, 141, 279, 342
PspN4I GGNNCC 4 cut(s) 108, 109, 140, 305
PspPI GGNCC 2 cut(s) 107, 303
PspPPI RGGWCCY 2 cut(s) 107, 303
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 2 cut(s) 120, 438
RsaNI GTAC 2 cut(s) 119, 437
SacI GAGCTC 1 cut(s) 258
SacII CCGCGG 1 cut(s) 82
SatI GCNGC 3 cut(s) 82, 224, 389
Sau3AI GATC 3 cut(s) 4, 51, 394
Sau96I GGNCC 2 cut(s) 107, 303
ScrFI CCNGG 5 cut(s) 17, 105, 143, 281, 344
SduI GDGCHC 3 cut(s) 258, 273, 375
Sfr303I CCGCGG 1 cut(s) 82
SgrAI CRCCGGYG 1 cut(s) 332
SgrBI CCGCGG 1 cut(s) 82
SinI GGWCC 2 cut(s) 107, 303
Sse9I AATT 3 cut(s) 89, 191, 290
SsiI CCGC 7 cut(s) 79, 81, 101, 214, 379, 388, 440
SstI GAGCTC 1 cut(s) 258
StyD4I CCNGG 5 cut(s) 15, 103, 141, 279, 342
StyI CCWWGG 1 cut(s) 261
TaaI ACNGT 3 cut(s) 167, 361, 404
TaiI ACGT 1 cut(s) 315
TaqI TCGA 1 cut(s) 299
TasI AATT 3 cut(s) 89, 191, 290
TauI GCSGC 2 cut(s) 84, 391
TfiI GAWTC 1 cut(s) 453
TscAI CASTG 1 cut(s) 380
TseI GCWGC 1 cut(s) 223
TspRI CASTG 1 cut(s) 380
Van91I CCANNNNNTGG 1 cut(s) 349
VpaK11BI GGWCC 2 cut(s) 107, 303
XapI RAATTY 3 cut(s) 89, 191, 290
XmiI GTMKAC 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.