MD03G1058900.v1.1

Belongs to the peroxidase family. Classical plant (class III) peroxidase subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005911
Physical Location & Seq
Forward (+)
4745447 .. 4749224
3778 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1058900.v1.1.491

Sequence Viewer

Length: 1152 bp
ATGCGGCAAGTCAAATTTTGGAGAAGTTGTTCTTTTGTGATCAAAAGGTCACGGGTTCGAGTCGTGGAAACAACCTCCTGCTTTGCAAGGATAATAGATGTTCCTCTTCCGAACCTCGCAAAGCGGGGACTCCATTCTACTGGAGAAAGTAAAGAGGAAAAGAAGTACATTGTCCCCTCTCTTTTTTCAGCTATGGCTTTTATCTCACACACTATCAGATTCTGCTTCTTTGTGCTTTTATTCCTGTTTAGAATATCGTCTGCTCAATTGTCCTCCAATCACTACGCGACAACGTGCCCCCGAGCCCTTTCCATTGTTCGGAATGCAGTTATTAAAGCAATGGTGAAGGAGCAGCGCATGGGGGCATCCTTGCTCCGACTCCATTTCCACGATTGCTTTGTCAATGGATGTGACGCATCTGTTCTATTAGATGACACTGCAAATTTTACTGGGGAAAAGACGGCGTTACCAAATACAAACTCATTGAGGGGATTTGAAGTGATTGATACTATAAAATCTGAGCTAGAAAGTGCCTGCCCTGGGGTTGTTTCCTGTGCAGATATCCTTGCCATTGCAGCTCGCGATTCAGTTCTTTCGTTGGGAGGGCCTTCATGGACAGTTCAATTGGGCAGAAGAGACTCCACCACAGCAAGTTTAAGTGCTGCAAATGCTGAAATCCCATCTCCAAGTCTAGATCTTAATAACCTCATCTCTTTTTTCTCAACTAAAGGATTTACTACCAAAGAATTGGTAGCTCTCTCTGGATCTCATACAATGGGTCAAGCAAGGTGTCTAGTGTTTCGAAAACGGATACATAATGAGACCAACATCGATTCATCGTTTGCTACATCTTTGAAATCAAACTGTACTGCGAGCTCCGGGACTGATGACAACCTTTCTTCACTAGATGTTACAAGCCCTTTCATATTCGACAACGCTTATTTTAAGAACCTGGTGAATAGAAAGGGTCTATTGCATTCTGATCAGCAGCTTTTCAGTGGTGGATCAACAGATTCTCTGGTTACAACTTATAGCACCACTGCCGGAACTTTTTATACAGATTTTGCAAATGCCATGGTGAAAATGGGGAGCCTTAGCCCGCTTACTGGAACGACTGGTCAAGTTCGTACCAACTGCCGCAAAATAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
peroxidase

Protein Analysis

384

Amino Acids

41.81

Weight (kDa)

9.21

Isoelectric Point (pI)

37.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
peroxidase PF00141 106 - 345 3e-84 Peroxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 287, 582
AciI CCGC 4 cut(s) 4, 124, 1100, 1138
AclWI GGATC 2 cut(s) 772, 1012
AcsI RAATTY 2 cut(s) 14, 442
AfaI GTAC 3 cut(s) 167, 868, 1129
AfiI CCNNNNNNNGG 3 cut(s) 318, 540, 1106
AgsI TTSAA 3 cut(s) 497, 623, 856
AjnI CCWGG 2 cut(s) 538, 951
AloI GAACNNNNNNTCC 2 cut(s) 13, 45
AluBI AGCT 6 cut(s) 191, 523, 578, 755, 876, 991
AluI AGCT 6 cut(s) 191, 523, 578, 755, 876, 991
Alw21I GWGCWC 1 cut(s) 878
Alw26I GTCTC 2 cut(s) 630, 815
AlwI GGATC 2 cut(s) 772, 1012
AlwNI CAGNNNCTG 1 cut(s) 222
Ama87I CYCGRG 1 cut(s) 300
AoxI GGCC 1 cut(s) 605
ApeKI GCWGC 4 cut(s) 352, 575, 662, 988
ApoI RAATTY 2 cut(s) 14, 442
Asp700I GAANNNNTTC 1 cut(s) 28
AspLEI GCGC 1 cut(s) 357
AspS9I GGNCC 1 cut(s) 605
AsuC2I CCSGG 1 cut(s) 880
AsuHPI GGTGA 3 cut(s) 355, 967, 1090
AsuII TTCGAA 1 cut(s) 802
AvaI CYCGRG 1 cut(s) 300
BaeGI GKGCMC 1 cut(s) 299
BanII GRGCYC 2 cut(s) 307, 878
Bbv12I GWGCWC 1 cut(s) 878
BbvI GCAGC 4 cut(s) 364, 587, 649, 1000
BccI CCATC 1 cut(s) 688
BceAI ACGGC 1 cut(s) 477
BciT130I CCWGG 2 cut(s) 540, 953
BciVI GTATCC 1 cut(s) 804
BclI TGATCA 2 cut(s) 39, 982
BcnI CCSGG 1 cut(s) 880
BcoDI GTCTC 2 cut(s) 630, 815
BfaI CTAG 4 cut(s) 524, 692, 794, 905
BfuI GTATCC 1 cut(s) 804
BglII AGATCT 1 cut(s) 694
BisI GCNGC 6 cut(s) 5, 353, 576, 663, 989, 1138
BlsI GCNGC 6 cut(s) 6, 354, 577, 664, 990, 1139
Bme1390I CCNGG 3 cut(s) 540, 880, 953
BmeT110I CYCGRG 1 cut(s) 300
BmgT120I GGNCC 1 cut(s) 605
BmiI GGNNCC 1 cut(s) 1091
BmrFI CCNGG 3 cut(s) 540, 880, 953
BmrI ACTGGG 1 cut(s) 459
BmsI GCATC 2 cut(s) 374, 425
BmuI ACTGGG 1 cut(s) 459
BpmI CTGGAG 1 cut(s) 162
Bpu10I CCTNAGC 1 cut(s) 1094
Bpu14I TTCGAA 1 cut(s) 802
BpuMI CCSGG 1 cut(s) 880
Bsa29I ATCGAT 1 cut(s) 831
BsaI GGTCTC 1 cut(s) 815
BsaJI CCNNGG 3 cut(s) 538, 539, 1074
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bsc4I CCNNNNNNNGG 3 cut(s) 318, 540, 1106
Bse1I ACTGG 4 cut(s) 145, 454, 1111, 1120
Bse3DI GCAATG 2 cut(s) 345, 570
BseBI CCWGG 2 cut(s) 540, 953
BseCI ATCGAT 1 cut(s) 831
BseDI CCNNGG 3 cut(s) 538, 539, 1074
BseGI GGATG 2 cut(s) 365, 413
BseLI CCNNNNNNNGG 3 cut(s) 318, 540, 1106
BseMI GCAATG 2 cut(s) 345, 570
BseMII CTCAG 1 cut(s) 510
BseNI ACTGG 4 cut(s) 145, 454, 1111, 1120
BseSI GKGCMC 1 cut(s) 299
BseXI GCAGC 4 cut(s) 364, 587, 649, 1000
BsgI GTGCAG 1 cut(s) 576
Bsh1236I CGCG 2 cut(s) 287, 582
BshFI GGCC 1 cut(s) 607
BshVI ATCGAT 1 cut(s) 831
BsiHKAI GWGCWC 1 cut(s) 878
BsiHKCI CYCGRG 1 cut(s) 300
BsiSI CCGG 2 cut(s) 879, 1044
BslFI GGGAC 3 cut(s) 141, 158, 895
BslI CCNNNNNNNGG 3 cut(s) 318, 540, 1106
BsmAI GTCTC 2 cut(s) 630, 815
BsmFI GGGAC 3 cut(s) 141, 158, 895
BsmI GAATGC 2 cut(s) 328, 976
BsnI GGCC 1 cut(s) 607
Bso31I GGTCTC 1 cut(s) 815
BsoBI CYCGRG 1 cut(s) 300
Bsp119I TTCGAA 1 cut(s) 802
Bsp1286I GDGCHC 3 cut(s) 299, 307, 878
Bsp143I GATC 5 cut(s) 39, 694, 764, 982, 1004
Bsp19I CCATGG 1 cut(s) 1074
Bsp68I TCGCGA 1 cut(s) 582
BspACI CCGC 4 cut(s) 4, 124, 1100, 1138
BspANI GGCC 1 cut(s) 607
BspCNI CTCAG 1 cut(s) 511
BspDI ATCGAT 1 cut(s) 831
BspFNI CGCG 2 cut(s) 287, 582
BspLI GGNNCC 1 cut(s) 1091
BspPI GGATC 2 cut(s) 772, 1012
BspT104I TTCGAA 1 cut(s) 802
BspTNI GGTCTC 1 cut(s) 815
BsrDI GCAATG 2 cut(s) 345, 570
BsrI ACTGG 4 cut(s) 145, 454, 1111, 1120
BssECI CCNNGG 3 cut(s) 538, 539, 1074
BssMI GATC 5 cut(s) 39, 694, 764, 982, 1004
BssT1I CCWWGG 1 cut(s) 1074
Bst2UI CCWGG 2 cut(s) 540, 953
Bst4CI ACNGT 2 cut(s) 619, 866
Bst6I CTCTTC 2 cut(s) 111, 628
BstBI TTCGAA 1 cut(s) 802
BstC8I GCNNGC 4 cut(s) 535, 580, 874, 1100
BstDEI CTNAG 2 cut(s) 519, 1094
BstDSI CCRYGG 1 cut(s) 1074
BstF5I GGATG 2 cut(s) 365, 413
BstFNI CGCG 2 cut(s) 287, 582
BstHHI GCGC 1 cut(s) 357
BstKTI GATC 5 cut(s) 42, 697, 767, 985, 1007
BstMAI GTCTC 2 cut(s) 630, 815
BstMBI GATC 5 cut(s) 39, 694, 764, 982, 1004
BstMWI GCNNNNNNNGC 2 cut(s) 575, 668
BstNI CCWGG 2 cut(s) 540, 953
BstSCI CCNGG 3 cut(s) 538, 878, 951
BstSLI GKGCMC 1 cut(s) 299
BstUI CGCG 2 cut(s) 287, 582
BstV1I GCAGC 4 cut(s) 364, 587, 649, 1000
BstX2I RGATCY 2 cut(s) 694, 764
BstXI CCANNNNNNTGG 2 cut(s) 140, 748
BstYI RGATCY 2 cut(s) 694, 764
Bsu15I ATCGAT 1 cut(s) 831
BsuI GTATCC 1 cut(s) 804
BsuRI GGCC 1 cut(s) 607
BsuTUI ATCGAT 1 cut(s) 831
BtgI CCRYGG 1 cut(s) 1074
BtsCI GGATG 2 cut(s) 365, 413
BtsI GCAGTG 2 cut(s) 435, 1038
BtsIMutI CAGTG 3 cut(s) 435, 1003, 1038
BtuMI TCGCGA 1 cut(s) 582
Cac8I GCNNGC 4 cut(s) 535, 580, 874, 1100
CaiI CAGNNNCTG 1 cut(s) 222
CfoI GCGC 1 cut(s) 357
Cfr13I GGNCC 1 cut(s) 605
ClaI ATCGAT 1 cut(s) 831
CseI GACGC 1 cut(s) 422
CsiI ACCWGGT 1 cut(s) 951
Csp6I GTAC 3 cut(s) 166, 867, 1128
CviAII CATG 3 cut(s) 358, 612, 1075
CviQI GTAC 3 cut(s) 166, 867, 1128
DdeI CTNAG 2 cut(s) 519, 1094
DpnI GATC 5 cut(s) 41, 696, 766, 984, 1006
DpnII GATC 5 cut(s) 39, 694, 764, 982, 1004
Eam1104I CTCTTC 2 cut(s) 111, 628
EarI CTCTTC 2 cut(s) 111, 628
Ecl136II GAGCTC 1 cut(s) 876
Eco130I CCWWGG 1 cut(s) 1074
Eco24I GRGCYC 2 cut(s) 307, 878
Eco31I GGTCTC 1 cut(s) 815
Eco32I GATATC 1 cut(s) 562
Eco53kI GAGCTC 1 cut(s) 876
Eco88I CYCGRG 1 cut(s) 300
EcoICRI GAGCTC 1 cut(s) 876
EcoO109I RGGNCCY 1 cut(s) 605
EcoRII CCWGG 2 cut(s) 538, 951
EcoRV GATATC 1 cut(s) 562
EcoT14I CCWWGG 1 cut(s) 1074
EcoT38I GRGCYC 2 cut(s) 307, 878
ErhI CCWWGG 1 cut(s) 1074
FaeI CATG 3 cut(s) 361, 615, 1078
FalI AAGNNNNNCTT 2 cut(s) 16, 48
FaqI GGGAC 3 cut(s) 141, 158, 895
FatI CATG 3 cut(s) 357, 611, 1074
FauI CCCGC 2 cut(s) 117, 1107
FbaI TGATCA 2 cut(s) 39, 982
Fnu4HI GCNGC 6 cut(s) 5, 353, 576, 663, 989, 1138
FokI GGATG 2 cut(s) 352, 420
FriOI GRGCYC 2 cut(s) 307, 878
Fsp4HI GCNGC 6 cut(s) 5, 353, 576, 663, 989, 1138
FspBI CTAG 4 cut(s) 524, 692, 794, 905
GlaI GCGC 1 cut(s) 356
GluI GCNGC 6 cut(s) 5, 353, 576, 663, 989, 1138
GsuI CTGGAG 1 cut(s) 162
HaeIII GGCC 1 cut(s) 607
HapII CCGG 2 cut(s) 879, 1044
HgaI GACGC 1 cut(s) 422
HhaI GCGC 1 cut(s) 357
Hin1II CATG 3 cut(s) 361, 615, 1078
Hin6I GCGC 1 cut(s) 355
HinP1I GCGC 1 cut(s) 355
HinfI GANTC 8 cut(s) 60, 129, 219, 378, 584, 638, 833, 1013
HpaII CCGG 2 cut(s) 879, 1044
HphI GGTGA 3 cut(s) 355, 967, 1090
Hpy188I TCNGA 6 cut(s) 111, 218, 321, 377, 520, 982
Hpy188III TCNNGA 3 cut(s) 581, 692, 762
HpyAV CCTTC 2 cut(s) 340, 618
HpyCH4III ACNGT 2 cut(s) 619, 866
HpyCH4IV ACGT 1 cut(s) 293
HpyCH4V TGCA 8 cut(s) 86, 326, 440, 557, 575, 665, 976, 1067
HpyF10VI GCNNNNNNNGC 2 cut(s) 575, 668
HpyF3I CTNAG 2 cut(s) 519, 1094
HpySE526I ACGT 1 cut(s) 293
Hsp92II CATG 3 cut(s) 361, 615, 1078
HspAI GCGC 1 cut(s) 355
Ksp22I TGATCA 2 cut(s) 39, 982
Kzo9I GATC 5 cut(s) 39, 694, 764, 982, 1004
LmnI GCTCC 4 cut(s) 349, 378, 881, 1089
Lsp1109I GCAGC 4 cut(s) 364, 587, 649, 1000
LweI GCATC 2 cut(s) 374, 425
MabI ACCWGGT 1 cut(s) 951
MaeI CTAG 4 cut(s) 524, 692, 794, 905
MaeII ACGT 1 cut(s) 293
MaeIII GTNAC 5 cut(s) 48, 410, 465, 910, 1021
MalI GATC 5 cut(s) 41, 696, 766, 984, 1006
MboI GATC 5 cut(s) 39, 694, 764, 982, 1004
MboII GAAGA 3 cut(s) 98, 645, 891
MfeI CAATTG 2 cut(s) 266, 623
MflI RGATCY 2 cut(s) 694, 764
MhlI GDGCHC 3 cut(s) 299, 307, 878
MluCI AATT 5 cut(s) 14, 266, 442, 623, 746
MlyI GAGTC 4 cut(s) 69, 123, 372, 632
MmeI TCCRAC 1 cut(s) 400
MnlI CCTC 9 cut(s) 85, 114, 125, 148, 187, 283, 480, 596, 716
MroXI GAANNNNTTC 1 cut(s) 28
MseI TTAA 4 cut(s) 333, 656, 699, 945
MspI CCGG 2 cut(s) 879, 1044
MspR9I CCNGG 3 cut(s) 540, 880, 953
MunI CAATTG 2 cut(s) 266, 623
Mva1269I GAATGC 2 cut(s) 328, 976
MvaI CCWGG 2 cut(s) 540, 953
MvnI CGCG 2 cut(s) 287, 582
MwoI GCNNNNNNNGC 2 cut(s) 575, 668
NciI CCSGG 1 cut(s) 880
NcoI CCATGG 1 cut(s) 1074
NdeII GATC 5 cut(s) 39, 694, 764, 982, 1004
NlaIII CATG 3 cut(s) 361, 615, 1078
NlaIV GGNNCC 1 cut(s) 1091
NmuCI GTSAC 2 cut(s) 48, 410
NruI TCGCGA 1 cut(s) 582
NspV TTCGAA 1 cut(s) 802
PasI CCCWGGG 1 cut(s) 539
PctI GAATGC 2 cut(s) 328, 976
PdmI GAANNNNTTC 1 cut(s) 28
PfeI GAWTC 4 cut(s) 219, 584, 833, 1013
PfoI TCCNGGA 1 cut(s) 878
PkrI GCNGC 6 cut(s) 6, 354, 577, 664, 990, 1139
PleI GAGTC 4 cut(s) 68, 123, 372, 632
PpsI GAGTC 4 cut(s) 68, 123, 372, 632
Psp124BI GAGCTC 1 cut(s) 878
Psp6I CCWGG 2 cut(s) 538, 951
PspGI CCWGG 2 cut(s) 538, 951
PspN4I GGNNCC 1 cut(s) 1091
PspPI GGNCC 1 cut(s) 605
PsrI GAACNNNNNNTAC 2 cut(s) 1039, 1071
PstNI CAGNNNCTG 1 cut(s) 222
PsuI RGATCY 2 cut(s) 694, 764
RruI TCGCGA 1 cut(s) 582
RsaI GTAC 3 cut(s) 167, 868, 1129
RsaNI GTAC 3 cut(s) 166, 867, 1128
SacI GAGCTC 1 cut(s) 878
SaqAI TTAA 4 cut(s) 333, 656, 699, 945
SatI GCNGC 6 cut(s) 5, 353, 576, 663, 989, 1138
Sau3AI GATC 5 cut(s) 39, 694, 764, 982, 1004
Sau96I GGNCC 1 cut(s) 605
SchI GAGTC 4 cut(s) 69, 123, 372, 632
ScrFI CCNGG 3 cut(s) 540, 880, 953
SduI GDGCHC 3 cut(s) 299, 307, 878
SexAI ACCWGGT 1 cut(s) 951
SfaNI GCATC 2 cut(s) 374, 425
SfuI TTCGAA 1 cut(s) 802
Sse9I AATT 5 cut(s) 14, 266, 442, 623, 746
SsiI CCGC 4 cut(s) 4, 124, 1100, 1138
SspMI CTAG 4 cut(s) 524, 692, 794, 905
SstI GAGCTC 1 cut(s) 878
StyD4I CCNGG 3 cut(s) 538, 878, 951
StyI CCWWGG 1 cut(s) 1074
TaaI ACNGT 2 cut(s) 619, 866
TaiI ACGT 1 cut(s) 296
TaqI TCGA 4 cut(s) 58, 802, 831, 930
TasI AATT 5 cut(s) 14, 266, 442, 623, 746
TatI WGTACW 2 cut(s) 165, 866
TauI GCSGC 2 cut(s) 7, 1140
TfiI GAWTC 4 cut(s) 219, 584, 833, 1013
Tru1I TTAA 4 cut(s) 333, 656, 699, 945
Tru9I TTAA 4 cut(s) 333, 656, 699, 945
TscAI CASTG 3 cut(s) 442, 1003, 1045
TseFI GTSAC 2 cut(s) 48, 410
TseI GCWGC 4 cut(s) 352, 575, 662, 988
Tsp45I GTSAC 2 cut(s) 48, 410
TspDTI ATGAA 3 cut(s) 600, 825, 913
TspGWI ACGGA 1 cut(s) 823
TspRI CASTG 3 cut(s) 442, 1003, 1045
XapI RAATTY 2 cut(s) 14, 442
XbaI TCTAGA 1 cut(s) 691
XcmI CCANNNNNNNNNTGG 1 cut(s) 1081
XmnI GAANNNNTTC 1 cut(s) 28
XspI CTAG 4 cut(s) 524, 692, 794, 905
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.