Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0005272

Physical Location & Seq

Genomic Coordinates Forward (+)

5749224 .. 5750804

Gene Structure 1581 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1070400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1035 bp

ATGTTCATCCCATTGCTAGATCTATCAGTGAAGCAATTCCTGAAGACTAAATTAATAATTGGGAGAGTTCAAGAGAGAAATATGGGGTCAGAAACTCAGCCTCCTGTGGTTCCCGTTGTGGACTTATCCAGTGAAAACATGAAGCCTGGAACAGATGCATGGCTCTTGGCATGCAAAAAAGTCCAGCATGCCTTGGAAGAGTTCGGCTGCTTTGAAGCTGTTTACAAAAAAGATACTTCGGAAACTCAAAACTCCATTTTTTCTGCGATAAAAGATTTTTGTGATCTTCCATTAGAAACTAAAATGCAGAAGACCAGTGATAAGCCTCTCCACAGCTATTTTGGCCGAAATCCGCTCTTTCCTCACGATTTTTCCACTGGCATTGAGAATCCAGAAACTGTAGAAGGAGCTCAAAAGTTCACTAGTATCATGTGGCCTGCAGGAAATGAACGTTTTCGTGAAAGTGTTCATTCCTTCTCAAGGATGTTGGTAGAATTGGATCAAATGGTGACAAGAATGGTATTTGATATTTATGGGGTGGAGAGGCTCTATGACTCTCACAAGGCATCAACCACTTACCAGCTTAGATGCATTGAATATCAAATACAGGCCATACAGCAGACGGATAAGAGTGACGTGAGACTCTTTCCTCACACAGACAAGGGTTTCACAACCATACTGCATCAAAATGAGGTCAATGGTTTGCAGATCAAAACAAGGGCTGGCCAACGGATTGATTATGCACCTTCTTCACCTTCATCTTTTATAGTATTTGCAGCCGATGTACTCATGGCATGGAGTAACGACAGGGTATACGCAAGTGAGCATCAAGTCATCATGAAAGAGGAGAAAACAAGATATTCGGTGGCATTATTCTCATATAAAAATGGGATTGTGCAAGTACCTGAAGAGCTAGTTGATGACAAACACCCCTTACGATATAAGCCATTTGATCAATTTGATTACCTTCGTTTCTTTCAAACTGAGGAGGCAAAGAAAATTAAGCATCCTATCAAAGCCTACTGTGGTATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009653 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009791 GO:0009826 GO:0009908 GO:0009987 GO:0010033 GO:0010150 GO:0010228 GO:0010476 GO:0010817 GO:0016043 GO:0016049 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016491 GO:0016679 GO:0016682 GO:0016705 GO:0016706 GO:0016707 GO:0019752 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033993 GO:0040007 GO:0042221 GO:0042445 GO:0042446 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045544 GO:0046394 GO:0048366 GO:0048367 GO:0048572 GO:0048573 GO:0048575 GO:0048589 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051213 GO:0051716 GO:0055114 GO:0060560 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:0071840 GO:0090567 GO:0090693 GO:0099402 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

345

Amino Acids

39.68

Weight (kDa)

6.38

Isoelectric Point (pI)

32.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	37 - 116	1.2e-08	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	208 - 292	5.4e-18	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005272)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G52800
fragaria_vesca	FvH4_6g30750
malus_domestica	MD03G1070400.v1.1 MD11G1074400.v1.1 MD17G1199500.v1.1 MD17G1200100.v1.1 MD17G1200500.v1.1
prunus_persica	Prupe.3G076200_v2.0.a1
pyrus_communis	pycom03g05650 pycom03g05660 pycom15g25880 pycom15g25950
rosa_chinensis	RchiOBHm_Chr2g0137181 RchiOBHm_Chr5g0080031
rosa_laevigata	RLG00000019632 RLG00000036847
rosa_multiflora	Rmu_sc0000894.1_g000045
rosa_roxburghii	Rroxscaffold_1G00003110 Rroxscaffold_2G00107410
rosa_rugosa	Rorug05G0467900
rosa_samantha	Rh2AG388000 Rh2BG395800 Rh2CG375300 Rh2DG410800 Rh5AG521400 Rh5CG566900 Rh5DG553500
rosa_wichuraiana	Rw2G031800 Rw5G048390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	355
AccI	GTMKAC	1 cut(s)	813
AciI	CCGC	1 cut(s)	353
AclI	AACGTT	1 cut(s)	451
AclWI	GGATC	1 cut(s)	507
AcoI	YGGCCR	2 cut(s)	343, 724
AcuI	CTGAAG	2 cut(s)	62, 927
AfaI	GTAC	2 cut(s)	786, 903
AfiI	CCNNNNNNNGG	1 cut(s)	480
AgsI	TTSAA	4 cut(s)	71, 215, 596, 980
AhlI	ACTAGT	1 cut(s)	422
AjiI	CACGTC	1 cut(s)	637
AjnI	CCWGG	1 cut(s)	145
AluBI	AGCT	5 cut(s)	218, 336, 410, 583, 913
AluI	AGCT	5 cut(s)	218, 336, 410, 583, 913
Alw21I	GWGCWC	1 cut(s)	412
Alw26I	GTCTC	1 cut(s)	634
AlwI	GGATC	1 cut(s)	507
AlwNI	CAGNNNCTG	1 cut(s)	398
AoxI	GGCC	4 cut(s)	343, 434, 609, 724
ApeKI	GCWGC	2 cut(s)	207, 776
AseI	ATTAAT	1 cut(s)	53
Asp700I	GAANNNNTTC	3 cut(s)	35, 453, 465
AsuHPI	GGTGA	2 cut(s)	520, 744
BalI	TGGCCA	1 cut(s)	726
BanII	GRGCYC	1 cut(s)	412
BbsI	GAAGAC	2 cut(s)	50, 317
Bbv12I	GWGCWC	1 cut(s)	412
BbvI	GCAGC	2 cut(s)	194, 788
BciT130I	CCWGG	1 cut(s)	147
BclI	TGATCA	1 cut(s)	952
BcoDI	GTCTC	1 cut(s)	634
BcuI	ACTAGT	1 cut(s)	422
BfaI	CTAG	3 cut(s)	17, 423, 914
BfmI	CTRYAG	2 cut(s)	399, 438
BglII	AGATCT	1 cut(s)	19
BisI	GCNGC	2 cut(s)	208, 777
BlsI	GCNGC	2 cut(s)	209, 778
Bme1390I	CCNGG	1 cut(s)	147
BmgBI	CACGTC	1 cut(s)	637
BmiI	GGNNCC	1 cut(s)	111
BmrFI	CCNGG	1 cut(s)	147
BmsI	GCATC	6 cut(s)	145, 575, 578, 691, 835, 1015
BpiI	GAAGAC	2 cut(s)	50, 317
BpuEI	CTTGAG	1 cut(s)	463
BsaJI	CCNNGG	1 cut(s)	192
BsaXI	ACNNNNNCTCC	2 cut(s)	85, 115
Bsc4I	CCNNNNNNNGG	1 cut(s)	480
Bse1I	ACTGG	3 cut(s)	129, 315, 382
Bse3DI	GCAATG	1 cut(s)	11
BseBI	CCWGG	1 cut(s)	147
BseDI	CCNNGG	1 cut(s)	192
BseGI	GGATG	3 cut(s)	6, 489, 1006
BseLI	CCNNNNNNNGG	1 cut(s)	480
BseMI	GCAATG	1 cut(s)	11
BseMII	CTCAG	2 cut(s)	110, 975
BseNI	ACTGG	3 cut(s)	129, 315, 382
BseRI	GAGGAG	2 cut(s)	860, 1001
BseXI	GCAGC	2 cut(s)	194, 788
BshFI	GGCC	4 cut(s)	345, 436, 611, 726
BsiHKAI	GWGCWC	1 cut(s)	412
BslI	CCNNNNNNNGG	1 cut(s)	480
BsmAI	GTCTC	1 cut(s)	634
BsnI	GGCC	4 cut(s)	345, 436, 611, 726
Bsp1286I	GDGCHC	1 cut(s)	412
Bsp143I	GATC	5 cut(s)	19, 283, 499, 708, 952
BspACI	CCGC	1 cut(s)	353
BspANI	GGCC	4 cut(s)	345, 436, 611, 726
BspCNI	CTCAG	2 cut(s)	109, 976
BspHI	TCATGA	1 cut(s)	837
BspLI	GGNNCC	1 cut(s)	111
BspMAI	CTGCAG	1 cut(s)	442
BspPI	GGATC	1 cut(s)	507
BspQI	GCTCTTC	1 cut(s)	903
BsrBI	CCGCTC	1 cut(s)	355
BsrDI	GCAATG	1 cut(s)	11
BsrI	ACTGG	3 cut(s)	129, 315, 382
BssECI	CCNNGG	1 cut(s)	192
BssMI	GATC	5 cut(s)	19, 283, 499, 708, 952
BssNAI	GTATAC	1 cut(s)	814
BssT1I	CCWWGG	1 cut(s)	192
Bst1107I	GTATAC	1 cut(s)	814
Bst2UI	CCWGG	1 cut(s)	147
Bst4CI	ACNGT	2 cut(s)	400, 1025
Bst6I	CTCTTC	2 cut(s)	192, 903
BstC8I	GCNNGC	4 cut(s)	172, 189, 438, 724
BstDEI	CTNAG	3 cut(s)	96, 584, 984
BstENI	CCTNNNNNAGG	1 cut(s)	478
BstF5I	GGATG	3 cut(s)	6, 489, 1006
BstKTI	GATC	5 cut(s)	22, 286, 502, 711, 955
BstMAI	GTCTC	1 cut(s)	634
BstMBI	GATC	5 cut(s)	19, 283, 499, 708, 952
BstMWI	GCNNNNNNNGC	1 cut(s)	342
BstNI	CCWGG	1 cut(s)	147
BstNSI	RCATGY	2 cut(s)	174, 191
BstSCI	CCNGG	1 cut(s)	145
BstSFI	CTRYAG	2 cut(s)	399, 438
BstV1I	GCAGC	2 cut(s)	194, 788
BstV2I	GAAGAC	2 cut(s)	50, 317
BstX2I	RGATCY	1 cut(s)	19
BstYI	RGATCY	1 cut(s)	19
BstZ17I	GTATAC	1 cut(s)	814
BsuRI	GGCC	4 cut(s)	345, 436, 611, 726
BtrI	CACGTC	1 cut(s)	637
BtsCI	GGATG	3 cut(s)	6, 489, 1006
BtsIMutI	CAGTG	4 cut(s)	33, 136, 322, 375
Cac8I	GCNNGC	4 cut(s)	172, 189, 438, 724
CaiI	CAGNNNCTG	1 cut(s)	398
CciI	TCATGA	1 cut(s)	837
Csp6I	GTAC	2 cut(s)	785, 902
CspCI	CAANNNNNGTGG	2 cut(s)	364, 399
CviAII	CATG	8 cut(s)	139, 159, 171, 188, 430, 790, 795, 838
CviQI	GTAC	2 cut(s)	785, 902
DdeI	CTNAG	3 cut(s)	96, 584, 984
DpnI	GATC	5 cut(s)	21, 285, 501, 710, 954
DpnII	GATC	5 cut(s)	19, 283, 499, 708, 952
EaeI	YGGCCR	2 cut(s)	343, 724
Eam1104I	CTCTTC	2 cut(s)	192, 903
EarI	CTCTTC	2 cut(s)	192, 903
Ecl136II	GAGCTC	1 cut(s)	410
Eco130I	CCWWGG	1 cut(s)	192
Eco24I	GRGCYC	1 cut(s)	412
Eco53kI	GAGCTC	1 cut(s)	410
Eco57I	CTGAAG	2 cut(s)	62, 927
EcoICRI	GAGCTC	1 cut(s)	410
EcoNI	CCTNNNNNAGG	1 cut(s)	478
EcoRII	CCWGG	1 cut(s)	145
EcoT14I	CCWWGG	1 cut(s)	192
EcoT22I	ATGCAT	2 cut(s)	160, 593
EcoT38I	GRGCYC	1 cut(s)	412
ErhI	CCWWGG	1 cut(s)	192
FaeI	CATG	8 cut(s)	142, 162, 174, 191, 433, 793, 798, 841
FatI	CATG	8 cut(s)	138, 158, 170, 187, 429, 789, 794, 837
FbaI	TGATCA	1 cut(s)	952
FblI	GTMKAC	1 cut(s)	813
Fnu4HI	GCNGC	2 cut(s)	208, 777
FokI	GGATG	2 cut(s)	496, 993
FriOI	GRGCYC	1 cut(s)	412
Fsp4HI	GCNGC	2 cut(s)	208, 777
FspBI	CTAG	3 cut(s)	17, 423, 914
GluI	GCNGC	2 cut(s)	208, 777
HaeIII	GGCC	4 cut(s)	345, 436, 611, 726
Hin1II	CATG	8 cut(s)	142, 162, 174, 191, 433, 793, 798, 841
HinfI	GANTC	3 cut(s)	388, 554, 642
HphI	GGTGA	2 cut(s)	520, 744
Hpy166II	GTNNAC	4 cut(s)	121, 223, 420, 814
Hpy188I	TCNGA	2 cut(s)	91, 241
Hpy188III	TCNNGA	6 cut(s)	40, 71, 365, 392, 458, 838
Hpy8I	GTNNAC	4 cut(s)	121, 223, 420, 814
HpyAV	CCTTC	5 cut(s)	398, 484, 756, 765, 977
HpyCH4III	ACNGT	2 cut(s)	400, 1025
HpyCH4IV	ACGT	2 cut(s)	451, 636
HpyF10VI	GCNNNNNNNGC	1 cut(s)	342
HpyF3I	CTNAG	3 cut(s)	96, 584, 984
HpySE526I	ACGT	2 cut(s)	451, 636
Hsp92II	CATG	8 cut(s)	142, 162, 174, 191, 433, 793, 798, 841
Ksp22I	TGATCA	1 cut(s)	952
Kzo9I	GATC	5 cut(s)	19, 283, 499, 708, 952
LguI	GCTCTTC	1 cut(s)	903
LmnI	GCTCC	1 cut(s)	407
Lsp1109I	GCAGC	2 cut(s)	194, 788
LweI	GCATC	6 cut(s)	145, 575, 578, 691, 835, 1015
MaeI	CTAG	3 cut(s)	17, 423, 914
MaeII	ACGT	2 cut(s)	451, 636
MaeIII	GTNAC	3 cut(s)	508, 632, 800
MalI	GATC	5 cut(s)	21, 285, 501, 710, 954
MbiI	CCGCTC	1 cut(s)	355
MboI	GATC	5 cut(s)	19, 283, 499, 708, 952
MboII	GAAGA	6 cut(s)	55, 209, 278, 322, 741, 920
MflI	RGATCY	1 cut(s)	19
MhlI	GDGCHC	1 cut(s)	412
MlsI	TGGCCA	1 cut(s)	726
MluCI	AATT	6 cut(s)	35, 50, 57, 494, 956, 999
MluNI	TGGCCA	1 cut(s)	726
MlyI	GAGTC	2 cut(s)	548, 636
MnlI	CCTC	9 cut(s)	111, 336, 372, 537, 660, 685, 838, 979, 982
Mox20I	TGGCCA	1 cut(s)	726
Mph1103I	ATGCAT	2 cut(s)	160, 593
MroXI	GAANNNNTTC	3 cut(s)	35, 453, 465
MscI	TGGCCA	1 cut(s)	726
MseI	TTAA	2 cut(s)	53, 1002
MslI	CAYNNNNRTG	1 cut(s)	687
Msp20I	TGGCCA	1 cut(s)	726
MspR9I	CCNGG	1 cut(s)	147
MvaI	CCWGG	1 cut(s)	147
MwoI	GCNNNNNNNGC	1 cut(s)	342
NdeII	GATC	5 cut(s)	19, 283, 499, 708, 952
NlaIII	CATG	8 cut(s)	142, 162, 174, 191, 433, 793, 798, 841
NlaIV	GGNNCC	1 cut(s)	111
NmuCI	GTSAC	2 cut(s)	508, 632
NsiI	ATGCAT	2 cut(s)	160, 593
NspI	RCATGY	2 cut(s)	174, 191
PaeI	GCATGC	2 cut(s)	174, 191
PagI	TCATGA	1 cut(s)	837
PciSI	GCTCTTC	1 cut(s)	903
PdmI	GAANNNNTTC	3 cut(s)	35, 453, 465
PfeI	GAWTC	1 cut(s)	388
PkrI	GCNGC	2 cut(s)	209, 778
PleI	GAGTC	2 cut(s)	548, 636
PpsI	GAGTC	2 cut(s)	548, 636
PshBI	ATTAAT	1 cut(s)	53
Psp124BI	GAGCTC	1 cut(s)	412
Psp1406I	AACGTT	1 cut(s)	451
Psp6I	CCWGG	1 cut(s)	145
PspGI	CCWGG	1 cut(s)	145
PspN4I	GGNNCC	1 cut(s)	111
PstI	CTGCAG	1 cut(s)	442
PstNI	CAGNNNCTG	1 cut(s)	398
PsuI	RGATCY	1 cut(s)	19
RsaI	GTAC	2 cut(s)	786, 903
RsaNI	GTAC	2 cut(s)	785, 902
RseI	CAYNNNNRTG	1 cut(s)	687
SacI	GAGCTC	1 cut(s)	412
SapI	GCTCTTC	1 cut(s)	903
SaqAI	TTAA	2 cut(s)	53, 1002
SatI	GCNGC	2 cut(s)	208, 777
Sau3AI	GATC	5 cut(s)	19, 283, 499, 708, 952
SbfI	CCTGCAGG	1 cut(s)	442
SchI	GAGTC	2 cut(s)	548, 636
ScrFI	CCNGG	1 cut(s)	147
SdaI	CCTGCAGG	1 cut(s)	442
SduI	GDGCHC	1 cut(s)	412
SfaNI	GCATC	6 cut(s)	145, 575, 578, 691, 835, 1015
SfcI	CTRYAG	2 cut(s)	399, 438
SmiMI	CAYNNNNRTG	1 cut(s)	687
SmlI	CTYRAG	1 cut(s)	478
SmoI	CTYRAG	1 cut(s)	478
SpeI	ACTAGT	1 cut(s)	422
SphI	GCATGC	2 cut(s)	174, 191
Sse8387I	CCTGCAGG	1 cut(s)	442
Sse9I	AATT	6 cut(s)	35, 50, 57, 494, 956, 999
SsiI	CCGC	1 cut(s)	353
SspMI	CTAG	3 cut(s)	17, 423, 914
SstI	GAGCTC	1 cut(s)	412
StyD4I	CCNGG	1 cut(s)	145
StyI	CCWWGG	1 cut(s)	192
TaaI	ACNGT	2 cut(s)	400, 1025
TaiI	ACGT	2 cut(s)	454, 639
TasI	AATT	6 cut(s)	35, 50, 57, 494, 956, 999
TatI	WGTACW	1 cut(s)	784
TfiI	GAWTC	1 cut(s)	388
Tru1I	TTAA	2 cut(s)	53, 1002
Tru9I	TTAA	2 cut(s)	53, 1002
TscAI	CASTG	4 cut(s)	33, 136, 322, 382
TseFI	GTSAC	2 cut(s)	508, 632
TseI	GCWGC	2 cut(s)	207, 776
Tsp45I	GTSAC	2 cut(s)	508, 632
TspDTI	ATGAA	5 cut(s)	155, 458, 462, 747, 854
TspGWI	ACGGA	2 cut(s)	638, 745
TspRI	CASTG	4 cut(s)	33, 136, 322, 382
VspI	ATTAAT	1 cut(s)	53
XagI	CCTNNNNNAGG	1 cut(s)	478
XceI	RCATGY	2 cut(s)	174, 191
XcmI	CCANNNNNNNNNTGG	1 cut(s)	338
XmiI	GTMKAC	1 cut(s)	813
XmnI	GAANNNNTTC	3 cut(s)	35, 453, 465
XspI	CTAG	3 cut(s)	17, 423, 914
Zsp2I	ATGCAT	2 cut(s)	160, 593

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.