Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0005272

Physical Location & Seq

Genomic Coordinates Reverse (-)

24227755 .. 24229466

Gene Structure 1712 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1200500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 939 bp

ATGGGGTCAGAAACTCAGCCTAAGCTTCCTGTTATAGATTTATCTGATGAAAACTTAAAGCCCGGTACAGATGCATGGCTCTTGGCATGCAAAGAAATCAAGGATGCACTAGAAGAGTATGGCTGCTTCGAAGCTATTTACCATAAAATTCCTCTGGAACTTCACAACTCCATTTTTTCTGCGATGGAAGATCTATTTGGTCTTCCATTAGAAACTAAAAAGCAAAAAACCAGTGATAGGCCTTACCATAGCTATTTTGGACAATATTCATTCCTTCCTCTCTACGAATCCCTGGGGGTCGATAATCCAACAACTGTTGAAGGAGCTCAAGGTTTCACAAGCATCATGTGGCCTCAAGGAAATGCCCATTTTTGTGAAACTGCTCATTCCTTCTCAAAGCTCGTAGCAGAATTGAGTCAAATGGTGACAAGAATGGTTTTTGATATGTACGGTGTGCAGAGGCTTTTTGACTCTCACATGGAATCAACTACATACCTGCTTAGATGCTTTAAGTACAGAACACAAGAAGAGAATGAAACCAATGTGGGACTGCATCCTCACACAGACAAGACCTTCATATCCATACTGAATCAAAATCAGGTGAATGGCTTGCAGATTGGAACAAAGGATGGTCAGTGGATTGATGTGAACCCTTCACCCTCATCTTTTCTAATTTTGGCAGGCGATGCATTCATGGCATGGAGCAATGACAGAGTACCCTCTTGTGATCATCAAGTCACCATGAAAGAGAACAAAACCAGATACTCCCTAGGACTGTTCGCATTCAAAAGCGGGATTATACAAGTACCTGAAGAGCTAATTGATGAGAAGCATCCCTTGTTGTATAAACCATTTGATCATTTCAGTTTCCTTGACTTCGATCGAACACCAGAGGCAAGGAAATTGAAGTGTTCTATCAAAGCCTACTGTGGTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009314 GO:0009416 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009987 GO:0010033 GO:0010476 GO:0010817 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016491 GO:0016705 GO:0016706 GO:0016707 GO:0019752 GO:0023052 GO:0032870 GO:0033993 GO:0042221 GO:0042445 GO:0042446 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0046394 GO:0050789 GO:0050794 GO:0050896 GO:0051213 GO:0051716 GO:0055114 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

313

Amino Acids

35.58

Weight (kDa)

5.37

Isoelectric Point (pI)

28.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	9 - 87	2.3e-12	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	166 - 261	7.1e-25	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005272)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G52800
fragaria_vesca	FvH4_6g30750
malus_domestica	MD03G1070400.v1.1 MD11G1074400.v1.1 MD17G1199500.v1.1 MD17G1200100.v1.1 MD17G1200500.v1.1
prunus_persica	Prupe.3G076200_v2.0.a1
pyrus_communis	pycom03g05650 pycom03g05660 pycom15g25880 pycom15g25950
rosa_chinensis	RchiOBHm_Chr2g0137181 RchiOBHm_Chr5g0080031
rosa_laevigata	RLG00000019632 RLG00000036847
rosa_multiflora	Rmu_sc0000894.1_g000045
rosa_roxburghii	Rroxscaffold_1G00003110 Rroxscaffold_2G00107410
rosa_rugosa	Rorug05G0467900
rosa_samantha	Rh2AG388000 Rh2BG395800 Rh2CG375300 Rh2DG410800 Rh5AG521400 Rh5CG566900 Rh5DG553500
rosa_wichuraiana	Rw2G031800 Rw5G048390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	504
AciI	CCGC	1 cut(s)	792
AcsI	RAATTY	1 cut(s)	147
AcuI	CTGAAG	1 cut(s)	831
AfaI	GTAC	5 cut(s)	67, 449, 515, 717, 807
AfiI	CCNNNNNNNGG	1 cut(s)	237
AgsI	TTSAA	3 cut(s)	320, 787, 907
AjnI	CCWGG	1 cut(s)	291
AjuI	GAANNNNNNNTTGG	2 cut(s)	180, 212
AluBI	AGCT	6 cut(s)	25, 134, 252, 326, 400, 817
AluI	AGCT	6 cut(s)	25, 134, 252, 326, 400, 817
Alw21I	GWGCWC	1 cut(s)	328
AoxI	GGCC	2 cut(s)	239, 350
ApeKI	GCWGC	1 cut(s)	123
ApoI	RAATTY	1 cut(s)	147
AspA2I	CCTAGG	1 cut(s)	769
AsuC2I	CCSGG	1 cut(s)	63
AsuHPI	GGTGA	4 cut(s)	436, 613, 648, 730
AsuII	TTCGAA	1 cut(s)	129
AvrII	CCTAGG	1 cut(s)	769
BanII	GRGCYC	1 cut(s)	328
BarI	GAAGNNNNNNTAC	2 cut(s)	110, 142
BbsI	GAAGAC	1 cut(s)	194
Bbv12I	GWGCWC	1 cut(s)	328
BbvI	GCAGC	1 cut(s)	110
BccI	CCATC	2 cut(s)	178, 623
BciT130I	CCWGG	1 cut(s)	293
BclI	TGATCA	2 cut(s)	727, 856
BcnI	CCSGG	1 cut(s)	63
BfaI	CTAG	2 cut(s)	110, 770
BfuAI	ACCTGC	1 cut(s)	504
BglII	AGATCT	1 cut(s)	190
BisI	GCNGC	1 cut(s)	124
BlnI	CCTAGG	1 cut(s)	769
BlsI	GCNGC	1 cut(s)	125
Bme1390I	CCNGG	2 cut(s)	63, 293
BmrFI	CCNGG	2 cut(s)	63, 293
BmsI	GCATC	7 cut(s)	61, 94, 351, 494, 562, 676, 841
BpiI	GAAGAC	1 cut(s)	194
Bpu10I	CCTNAGC	1 cut(s)	21
Bpu14I	TTCGAA	1 cut(s)	129
BpuEI	CTTGAG	2 cut(s)	312, 339
BpuMI	CCSGG	1 cut(s)	63
BsaJI	CCNNGG	3 cut(s)	291, 292, 769
Bsc4I	CCNNNNNNNGG	1 cut(s)	237
Bse1I	ACTGG	1 cut(s)	231
Bse3DI	GCAATG	1 cut(s)	712
BseBI	CCWGG	1 cut(s)	293
BseDI	CCNNGG	3 cut(s)	291, 292, 769
BseGI	GGATG	4 cut(s)	109, 553, 634, 832
BseLI	CCNNNNNNNGG	1 cut(s)	237
BseMI	GCAATG	1 cut(s)	712
BseMII	CTCAG	1 cut(s)	29
BseNI	ACTGG	1 cut(s)	231
BseXI	GCAGC	1 cut(s)	110
BsgI	GTGCAG	1 cut(s)	476
Bsh1285I	CGRYCG	1 cut(s)	883
BshFI	GGCC	2 cut(s)	241, 352
BsiEI	CGRYCG	1 cut(s)	883
BsiHKAI	GWGCWC	1 cut(s)	328
BsiSI	CCGG	1 cut(s)	63
BslFI	GGGAC	1 cut(s)	561
BslI	CCNNNNNNNGG	1 cut(s)	237
BsmFI	GGGAC	1 cut(s)	561
BsmI	GAATGC	2 cut(s)	689, 782
BsnI	GGCC	2 cut(s)	241, 352
Bsp119I	TTCGAA	1 cut(s)	129
Bsp1286I	GDGCHC	1 cut(s)	328
Bsp143I	GATC	4 cut(s)	190, 727, 856, 880
BspACI	CCGC	1 cut(s)	792
BspANI	GGCC	2 cut(s)	241, 352
BspCNI	CTCAG	1 cut(s)	28
BspMI	ACCTGC	1 cut(s)	504
BspQI	GCTCTTC	1 cut(s)	807
BspT104I	TTCGAA	1 cut(s)	129
BsrDI	GCAATG	1 cut(s)	712
BsrI	ACTGG	1 cut(s)	231
BssECI	CCNNGG	3 cut(s)	291, 292, 769
BssMI	GATC	4 cut(s)	190, 727, 856, 880
BssT1I	CCWWGG	1 cut(s)	769
Bst2UI	CCWGG	1 cut(s)	293
Bst4CI	ACNGT	4 cut(s)	316, 452, 777, 929
Bst6I	CTCTTC	3 cut(s)	108, 522, 807
BstAPI	GCANNNNNTGC	1 cut(s)	686
BstBI	TTCGAA	1 cut(s)	129
BstC8I	GCNNGC	3 cut(s)	88, 611, 682
BstDEI	CTNAG	3 cut(s)	15, 21, 500
BstF5I	GGATG	4 cut(s)	109, 553, 634, 832
BstKTI	GATC	4 cut(s)	193, 730, 859, 883
BstMBI	GATC	4 cut(s)	190, 727, 856, 880
BstMCI	CGRYCG	1 cut(s)	883
BstMWI	GCNNNNNNNGC	2 cut(s)	686, 695
BstNI	CCWGG	1 cut(s)	293
BstNSI	RCATGY	1 cut(s)	90
BstSCI	CCNGG	2 cut(s)	61, 291
BstV1I	GCAGC	1 cut(s)	110
BstV2I	GAAGAC	1 cut(s)	194
BstX2I	RGATCY	1 cut(s)	190
BstYI	RGATCY	1 cut(s)	190
BsuRI	GGCC	2 cut(s)	241, 352
BtgZI	GCGATG	2 cut(s)	197, 699
BtsCI	GGATG	4 cut(s)	109, 553, 634, 832
BtsIMutI	CAGTG	2 cut(s)	238, 641
BveI	ACCTGC	1 cut(s)	504
Cac8I	GCNNGC	3 cut(s)	88, 611, 682
Csp6I	GTAC	5 cut(s)	66, 448, 514, 716, 806
CviAII	CATG	7 cut(s)	75, 87, 346, 478, 694, 699, 742
CviQI	GTAC	5 cut(s)	66, 448, 514, 716, 806
DdeI	CTNAG	3 cut(s)	15, 21, 500
DpnI	GATC	4 cut(s)	192, 729, 858, 882
DpnII	GATC	4 cut(s)	190, 727, 856, 880
Eam1104I	CTCTTC	3 cut(s)	108, 522, 807
EarI	CTCTTC	3 cut(s)	108, 522, 807
Ecl136II	GAGCTC	1 cut(s)	326
Eco130I	CCWWGG	1 cut(s)	769
Eco147I	AGGCCT	1 cut(s)	241
Eco24I	GRGCYC	1 cut(s)	328
Eco53kI	GAGCTC	1 cut(s)	326
Eco57I	CTGAAG	1 cut(s)	831
EcoICRI	GAGCTC	1 cut(s)	326
EcoRII	CCWGG	1 cut(s)	291
EcoT14I	CCWWGG	1 cut(s)	769
EcoT22I	ATGCAT	2 cut(s)	76, 691
EcoT38I	GRGCYC	1 cut(s)	328
ErhI	CCWWGG	1 cut(s)	769
FaeI	CATG	7 cut(s)	78, 90, 349, 481, 697, 702, 745
FalI	AAGNNNNNCTT	2 cut(s)	821, 853
FaqI	GGGAC	1 cut(s)	561
FatI	CATG	7 cut(s)	74, 86, 345, 477, 693, 698, 741
FauI	CCCGC	1 cut(s)	785
FbaI	TGATCA	2 cut(s)	727, 856
Fnu4HI	GCNGC	1 cut(s)	124
FokI	GGATG	4 cut(s)	116, 540, 641, 819
FriOI	GRGCYC	1 cut(s)	328
Fsp4HI	GCNGC	1 cut(s)	124
FspBI	CTAG	2 cut(s)	110, 770
GluI	GCNGC	1 cut(s)	124
HaeIII	GGCC	2 cut(s)	241, 352
HapII	CCGG	1 cut(s)	63
Hin1II	CATG	7 cut(s)	78, 90, 349, 481, 697, 702, 745
HindIII	AAGCTT	1 cut(s)	23
HinfI	GANTC	5 cut(s)	287, 415, 470, 482, 589
HpaII	CCGG	1 cut(s)	63
HphI	GGTGA	4 cut(s)	436, 613, 648, 730
Hpy166II	GTNNAC	1 cut(s)	649
Hpy188I	TCNGA	2 cut(s)	10, 46
Hpy188III	TCNNGA	1 cut(s)	155
Hpy8I	GTNNAC	1 cut(s)	649
HpyAV	CCTTC	5 cut(s)	284, 314, 400, 583, 663
HpyCH4III	ACNGT	4 cut(s)	316, 452, 777, 929
HpyCH4V	TGCA	7 cut(s)	74, 90, 107, 457, 553, 613, 689
HpyF10VI	GCNNNNNNNGC	2 cut(s)	686, 695
HpyF3I	CTNAG	3 cut(s)	15, 21, 500
Hsp92II	CATG	7 cut(s)	78, 90, 349, 481, 697, 702, 745
Ksp22I	TGATCA	2 cut(s)	727, 856
Kzo9I	GATC	4 cut(s)	190, 727, 856, 880
LguI	GCTCTTC	1 cut(s)	807
LmnI	GCTCC	2 cut(s)	323, 702
Lsp1109I	GCAGC	1 cut(s)	110
LweI	GCATC	7 cut(s)	61, 94, 351, 494, 562, 676, 841
MaeI	CTAG	2 cut(s)	110, 770
MaeIII	GTNAC	2 cut(s)	424, 736
MalI	GATC	4 cut(s)	192, 729, 858, 882
MboI	GATC	4 cut(s)	190, 727, 856, 880
MboII	GAAGA	5 cut(s)	125, 194, 200, 539, 824
MflI	RGATCY	1 cut(s)	190
MhlI	GDGCHC	1 cut(s)	328
MluCI	AATT	5 cut(s)	147, 410, 672, 819, 902
MlyI	GAGTC	2 cut(s)	424, 464
MmeI	TCCRAC	1 cut(s)	332
MnlI	CCTC	8 cut(s)	162, 288, 363, 453, 567, 670, 730, 886
Mph1103I	ATGCAT	2 cut(s)	76, 691
MseI	TTAA	2 cut(s)	56, 510
MslI	CAYNNNNRTG	1 cut(s)	372
MspI	CCGG	1 cut(s)	63
MspR9I	CCNGG	2 cut(s)	63, 293
Mva1269I	GAATGC	2 cut(s)	689, 782
MvaI	CCWGG	1 cut(s)	293
MwoI	GCNNNNNNNGC	2 cut(s)	686, 695
NciI	CCSGG	1 cut(s)	63
NdeII	GATC	4 cut(s)	190, 727, 856, 880
NlaIII	CATG	7 cut(s)	78, 90, 349, 481, 697, 702, 745
NmuCI	GTSAC	2 cut(s)	424, 736
NsiI	ATGCAT	2 cut(s)	76, 691
NspI	RCATGY	1 cut(s)	90
NspV	TTCGAA	1 cut(s)	129
PaeI	GCATGC	1 cut(s)	90
PasI	CCCWGGG	1 cut(s)	292
PceI	AGGCCT	1 cut(s)	241
PciSI	GCTCTTC	1 cut(s)	807
PctI	GAATGC	2 cut(s)	689, 782
PfeI	GAWTC	3 cut(s)	287, 482, 589
PkrI	GCNGC	1 cut(s)	125
Ple19I	CGATCG	1 cut(s)	883
PleI	GAGTC	2 cut(s)	423, 464
PpsI	GAGTC	2 cut(s)	423, 464
Psp124BI	GAGCTC	1 cut(s)	328
Psp6I	CCWGG	1 cut(s)	291
PspGI	CCWGG	1 cut(s)	291
PsuI	RGATCY	1 cut(s)	190
PvuI	CGATCG	1 cut(s)	883
RsaI	GTAC	5 cut(s)	67, 449, 515, 717, 807
RsaNI	GTAC	5 cut(s)	66, 448, 514, 716, 806
RseI	CAYNNNNRTG	1 cut(s)	372
SacI	GAGCTC	1 cut(s)	328
SapI	GCTCTTC	1 cut(s)	807
SaqAI	TTAA	2 cut(s)	56, 510
SatI	GCNGC	1 cut(s)	124
Sau3AI	GATC	4 cut(s)	190, 727, 856, 880
SchI	GAGTC	2 cut(s)	424, 464
ScrFI	CCNGG	2 cut(s)	63, 293
SduI	GDGCHC	1 cut(s)	328
SfaNI	GCATC	7 cut(s)	61, 94, 351, 494, 562, 676, 841
SfuI	TTCGAA	1 cut(s)	129
SmiMI	CAYNNNNRTG	1 cut(s)	372
SmlI	CTYRAG	2 cut(s)	327, 354
SmoI	CTYRAG	2 cut(s)	327, 354
SphI	GCATGC	1 cut(s)	90
Sse9I	AATT	5 cut(s)	147, 410, 672, 819, 902
SseBI	AGGCCT	1 cut(s)	241
SsiI	CCGC	1 cut(s)	792
SspI	AATATT	1 cut(s)	266
SspMI	CTAG	2 cut(s)	110, 770
SstI	GAGCTC	1 cut(s)	328
StuI	AGGCCT	1 cut(s)	241
StyD4I	CCNGG	2 cut(s)	61, 291
StyI	CCWWGG	1 cut(s)	769
TaaI	ACNGT	4 cut(s)	316, 452, 777, 929
TaqI	TCGA	4 cut(s)	129, 300, 879, 883
TasI	AATT	5 cut(s)	147, 410, 672, 819, 902
TatI	WGTACW	1 cut(s)	513
TfiI	GAWTC	3 cut(s)	287, 482, 589
Tru1I	TTAA	2 cut(s)	56, 510
Tru9I	TTAA	2 cut(s)	56, 510
TscAI	CASTG	2 cut(s)	238, 641
TseFI	GTSAC	2 cut(s)	424, 736
TseI	GCWGC	1 cut(s)	123
Tsp45I	GTSAC	2 cut(s)	424, 736
TspDTI	ATGAA	6 cut(s)	63, 258, 549, 565, 682, 758
TspRI	CASTG	2 cut(s)	238, 641
XapI	RAATTY	1 cut(s)	147
XceI	RCATGY	1 cut(s)	90
XcmI	CCANNNNNNNNNTGG	1 cut(s)	254
XmaJI	CCTAGG	1 cut(s)	769
XspI	CTAG	2 cut(s)	110, 770
Zsp2I	ATGCAT	2 cut(s)	76, 691

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.