Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0005272

Physical Location & Seq

Genomic Coordinates Reverse (-)

6312586 .. 6314406

Gene Structure 1821 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1074400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 948 bp

ATGGTGTCGAAAACTCAGCCTAAGGTGGTTCCTGTTGTAGACTTATCCGATGAAAACTTGAAGCCTGGAACAGATGCATGGCTCTTGGCATGCAAACAAGTCCAGCATGCACTAGAAGAGTACAGCTGCTTTGAAGCTGTTTACAATAAAGCTACTTTGGAAATTCACAACTCTATCTTTTCTGCGATAAAAGAACTTTGTGATCTTCCATTGGAAACTAAAATGCAGAAGGCCAGTGATAGGCCTTTCCACCACTATTTTGGCCAACTTCCACTCAATCCTCGCTATGAGACCTTGGGCATTCAAGATCCAGCATCCTTAGAAGGAGCTCAACGCTTCACAAGTATCATGTGGCCAGCAGGAAATGACCGTTTTTGTGTTCATTCCTTCTCAAAGGTGGTGGTAGAATTGGATCAAATGGTGAAACGAATGGTGTTTGATGCTTATGGTGTGGGGAGGCTTTATGATTCTTACAAGGCATCAACCACTTACCAGCTTAGATGCATTAATTATAAATCACAACTTACGAATGAGACTACTGATGTGGGACTGATTCCTCACACGGACAAGAATTTCACATCCATACTACACCAAAATGATGTCGGTGGTCTGCAGATCAAAACAAAGGATGGCGAGTGGATTGAGGCTGCACCTTCTTCACCTTCATCATTTCTAGTGTTGGCATCCGATTTATTCATGGCATGGAGTAACGACCGAATATCCGCCTGTGAGCATCAAGTCGTCCTGAAAGAGAAGAAAGCAAGATATTCACTGGCATTATTCTCATATATAGGTGATGGAGTTGTGCAGGTACCTGAAGAGTTTGTTGATGACAAACACCCCTTAAAATATAAGCCATTTGGTCATTTCGATTACCTTCGTTTCTACGAAACTGATGAGGAGGCCCGGAAATCTAAGAATCCTATCAAAGCCTACTGTGGTGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0001101 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009653 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009791 GO:0009826 GO:0009908 GO:0009987 GO:0010033 GO:0010150 GO:0010228 GO:0010476 GO:0010817 GO:0016043 GO:0016049 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016491 GO:0016679 GO:0016682 GO:0016705 GO:0016706 GO:0016707 GO:0019752 GO:0022414 GO:0023052 GO:0032501 GO:0032502 GO:0032870 GO:0032989 GO:0033993 GO:0040007 GO:0042221 GO:0042445 GO:0042446 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045544 GO:0046394 GO:0048366 GO:0048367 GO:0048572 GO:0048573 GO:0048575 GO:0048589 GO:0048608 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0051213 GO:0051716 GO:0055114 GO:0060560 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:0071840 GO:0090567 GO:0090693 GO:0099402 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

316

Amino Acids

35.82

Weight (kDa)

5.94

Isoelectric Point (pI)

32.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	10 - 87	3.5e-07	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	170 - 261	1e-19	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005272)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G52800
fragaria_vesca	FvH4_6g30750
malus_domestica	MD03G1070400.v1.1 MD11G1074400.v1.1 MD17G1199500.v1.1 MD17G1200100.v1.1 MD17G1200500.v1.1
prunus_persica	Prupe.3G076200_v2.0.a1
pyrus_communis	pycom03g05650 pycom03g05660 pycom15g25880 pycom15g25950
rosa_chinensis	RchiOBHm_Chr2g0137181 RchiOBHm_Chr5g0080031
rosa_laevigata	RLG00000019632 RLG00000036847
rosa_multiflora	Rmu_sc0000894.1_g000045
rosa_roxburghii	Rroxscaffold_1G00003110 Rroxscaffold_2G00107410
rosa_rugosa	Rorug05G0467900
rosa_samantha	Rh2AG388000 Rh2BG395800 Rh2CG375300 Rh2DG410800 Rh5AG521400 Rh5CG566900 Rh5DG553500
rosa_wichuraiana	Rw2G031800 Rw5G048390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	513
Acc36I	ACCTGC	1 cut(s)	799
Acc65I	GGTACC	1 cut(s)	811
AccB1I	GGYRCC	1 cut(s)	811
AccI	GTMKAC	1 cut(s)	39
AciI	CCGC	1 cut(s)	723
AclWI	GGATC	2 cut(s)	302, 420
AcoI	YGGCCR	2 cut(s)	262, 353
AcsI	RAATTY	2 cut(s)	162, 571
AcuI	CTGAAG	1 cut(s)	837
AfaI	GTAC	2 cut(s)	122, 813
AfiI	CCNNNNNNNGG	1 cut(s)	240
AgsI	TTSAA	3 cut(s)	61, 134, 305
AjnI	CCWGG	1 cut(s)	64
AluBI	AGCT	5 cut(s)	126, 137, 152, 329, 496
AluI	AGCT	5 cut(s)	126, 137, 152, 329, 496
Alw21I	GWGCWC	1 cut(s)	331
Alw26I	GTCTC	2 cut(s)	284, 527
AlwI	GGATC	2 cut(s)	302, 420
AoxI	GGCC	5 cut(s)	231, 242, 262, 353, 903
ApeKI	GCWGC	2 cut(s)	126, 647
ApoI	RAATTY	2 cut(s)	162, 571
AseI	ATTAAT	1 cut(s)	507
Asp718I	GGTACC	1 cut(s)	811
AspS9I	GGNCC	1 cut(s)	904
AsuC2I	CCSGG	1 cut(s)	907
AsuHPI	GGTGA	3 cut(s)	433, 651, 806
AxyI	CCTNAGG	1 cut(s)	21
BalI	TGGCCA	2 cut(s)	264, 355
BanI	GGYRCC	1 cut(s)	811
BanII	GRGCYC	1 cut(s)	331
Bbv12I	GWGCWC	1 cut(s)	331
BbvI	GCAGC	2 cut(s)	113, 634
BccI	CCATC	2 cut(s)	623, 791
BciT130I	CCWGG	1 cut(s)	66
BcnI	CCSGG	1 cut(s)	907
BcoDI	GTCTC	2 cut(s)	284, 527
BfaI	CTAG	2 cut(s)	113, 674
BfmI	CTRYAG	1 cut(s)	611
BfuAI	ACCTGC	1 cut(s)	799
BisI	GCNGC	2 cut(s)	127, 648
BlsI	GCNGC	2 cut(s)	128, 649
Bme1390I	CCNGG	2 cut(s)	66, 907
BmgT120I	GGNCC	1 cut(s)	904
BmiI	GGNNCC	2 cut(s)	30, 813
BmrFI	CCNGG	2 cut(s)	66, 907
BmsI	GCATC	7 cut(s)	64, 323, 430, 488, 491, 692, 742
BpuMI	CCSGG	1 cut(s)	907
BsaI	GGTCTC	1 cut(s)	284
BsaJI	CCNNGG	1 cut(s)	294
Bsc4I	CCNNNNNNNGG	1 cut(s)	240
Bse1I	ACTGG	2 cut(s)	234, 777
Bse21I	CCTNAGG	1 cut(s)	21
BseBI	CCWGG	1 cut(s)	66
BseDI	CCNNGG	1 cut(s)	294
BseGI	GGATG	4 cut(s)	314, 578, 634, 683
BseLI	CCNNNNNNNGG	1 cut(s)	240
BseMII	CTCAG	1 cut(s)	29
BseNI	ACTGG	2 cut(s)	234, 777
BseRI	GAGGAG	1 cut(s)	914
BseXI	GCAGC	2 cut(s)	113, 634
BsgI	GTGCAG	2 cut(s)	633, 827
Bsh1285I	CGRYCG	1 cut(s)	715
BshFI	GGCC	5 cut(s)	233, 244, 264, 355, 905
BshNI	GGYRCC	1 cut(s)	811
BsiEI	CGRYCG	1 cut(s)	715
BsiHKAI	GWGCWC	1 cut(s)	331
BsiSI	CCGG	1 cut(s)	907
BslFI	GGGAC	1 cut(s)	561
BslI	CCNNNNNNNGG	1 cut(s)	240
BsmAI	GTCTC	2 cut(s)	284, 527
BsmFI	GGGAC	1 cut(s)	561
BsmI	GAATGC	1 cut(s)	300
BsnI	GGCC	5 cut(s)	233, 244, 264, 355, 905
Bso31I	GGTCTC	1 cut(s)	284
Bsp1286I	GDGCHC	1 cut(s)	331
Bsp143I	GATC	4 cut(s)	202, 307, 412, 615
BspACI	CCGC	1 cut(s)	723
BspANI	GGCC	5 cut(s)	233, 244, 264, 355, 905
BspCNI	CTCAG	1 cut(s)	28
BspLI	GGNNCC	2 cut(s)	30, 813
BspMAI	CTGCAG	1 cut(s)	615
BspMI	ACCTGC	1 cut(s)	799
BspPI	GGATC	2 cut(s)	302, 420
BspT107I	GGYRCC	1 cut(s)	811
BspTNI	GGTCTC	1 cut(s)	284
BsrI	ACTGG	2 cut(s)	234, 777
BssECI	CCNNGG	1 cut(s)	294
BssMI	GATC	4 cut(s)	202, 307, 412, 615
BssT1I	CCWWGG	1 cut(s)	294
Bst2UI	CCWGG	1 cut(s)	66
Bst4CI	ACNGT	2 cut(s)	371, 938
Bst6I	CTCTTC	2 cut(s)	111, 813
BstC8I	GCNNGC	3 cut(s)	91, 108, 357
BstDEI	CTNAG	5 cut(s)	15, 21, 319, 497, 915
BstF5I	GGATG	4 cut(s)	314, 578, 634, 683
BstKTI	GATC	4 cut(s)	205, 310, 415, 618
BstMAI	GTCTC	2 cut(s)	284, 527
BstMBI	GATC	4 cut(s)	202, 307, 412, 615
BstMCI	CGRYCG	1 cut(s)	715
BstNI	CCWGG	1 cut(s)	66
BstNSI	RCATGY	2 cut(s)	93, 110
BstSCI	CCNGG	2 cut(s)	64, 905
BstSFI	CTRYAG	1 cut(s)	611
BstV1I	GCAGC	2 cut(s)	113, 634
BstX2I	RGATCY	1 cut(s)	307
BstXI	CCANNNNNNTGG	1 cut(s)	260
BstYI	RGATCY	1 cut(s)	307
Bsu36I	CCTNAGG	1 cut(s)	21
BsuRI	GGCC	5 cut(s)	233, 244, 264, 355, 905
BtsCI	GGATG	4 cut(s)	314, 578, 634, 683
BtsIMutI	CAGTG	2 cut(s)	241, 770
BveI	ACCTGC	1 cut(s)	799
Cac8I	GCNNGC	3 cut(s)	91, 108, 357
Cfr13I	GGNCC	1 cut(s)	904
Csp6I	GTAC	2 cut(s)	121, 812
CspCI	CAANNNNNGTGG	2 cut(s)	381, 416
CviAII	CATG	6 cut(s)	78, 90, 107, 349, 697, 702
CviQI	GTAC	2 cut(s)	121, 812
DdeI	CTNAG	5 cut(s)	15, 21, 319, 497, 915
DpnI	GATC	4 cut(s)	204, 309, 414, 617
DpnII	GATC	4 cut(s)	202, 307, 412, 615
EaeI	YGGCCR	2 cut(s)	262, 353
Eam1104I	CTCTTC	2 cut(s)	111, 813
EarI	CTCTTC	2 cut(s)	111, 813
EciI	GGCGGA	1 cut(s)	712
Ecl136II	GAGCTC	1 cut(s)	329
Eco130I	CCWWGG	1 cut(s)	294
Eco147I	AGGCCT	1 cut(s)	244
Eco24I	GRGCYC	1 cut(s)	331
Eco31I	GGTCTC	1 cut(s)	284
Eco53kI	GAGCTC	1 cut(s)	329
Eco57I	CTGAAG	1 cut(s)	837
Eco81I	CCTNAGG	1 cut(s)	21
EcoICRI	GAGCTC	1 cut(s)	329
EcoRII	CCWGG	1 cut(s)	64
EcoT14I	CCWWGG	1 cut(s)	294
EcoT22I	ATGCAT	2 cut(s)	79, 506
EcoT38I	GRGCYC	1 cut(s)	331
ErhI	CCWWGG	1 cut(s)	294
FaeI	CATG	6 cut(s)	81, 93, 110, 352, 700, 705
FaqI	GGGAC	1 cut(s)	561
FatI	CATG	6 cut(s)	77, 89, 106, 348, 696, 701
FblI	GTMKAC	1 cut(s)	39
Fnu4HI	GCNGC	2 cut(s)	127, 648
FokI	GGATG	4 cut(s)	301, 565, 641, 670
FriOI	GRGCYC	1 cut(s)	331
Fsp4HI	GCNGC	2 cut(s)	127, 648
FspBI	CTAG	2 cut(s)	113, 674
GluI	GCNGC	2 cut(s)	127, 648
HaeIII	GGCC	5 cut(s)	233, 244, 264, 355, 905
HapII	CCGG	1 cut(s)	907
Hin1II	CATG	6 cut(s)	81, 93, 110, 352, 700, 705
HinfI	GANTC	3 cut(s)	467, 553, 919
HpaII	CCGG	1 cut(s)	907
HphI	GGTGA	3 cut(s)	433, 651, 806
Hpy166II	GTNNAC	2 cut(s)	40, 142
Hpy188I	TCNGA	2 cut(s)	49, 688
Hpy188III	TCNNGA	2 cut(s)	305, 745
Hpy8I	GTNNAC	2 cut(s)	40, 142
HpyAV	CCTTC	6 cut(s)	223, 317, 397, 663, 672, 887
HpyCH4III	ACNGT	2 cut(s)	371, 938
HpyCH4V	TGCA	8 cut(s)	77, 93, 110, 226, 504, 613, 650, 808
HpyF3I	CTNAG	5 cut(s)	15, 21, 319, 497, 915
Hsp92II	CATG	6 cut(s)	81, 93, 110, 352, 700, 705
KpnI	GGTACC	1 cut(s)	815
Kzo9I	GATC	4 cut(s)	202, 307, 412, 615
LmnI	GCTCC	1 cut(s)	326
Lsp1109I	GCAGC	2 cut(s)	113, 634
LweI	GCATC	7 cut(s)	64, 323, 430, 488, 491, 692, 742
MaeI	CTAG	2 cut(s)	113, 674
MaeIII	GTNAC	1 cut(s)	707
MalI	GATC	4 cut(s)	204, 309, 414, 617
MboI	GATC	4 cut(s)	202, 307, 412, 615
MboII	GAAGA	5 cut(s)	128, 197, 648, 766, 830
MflI	RGATCY	1 cut(s)	307
MhlI	GDGCHC	1 cut(s)	331
MlsI	TGGCCA	2 cut(s)	264, 355
MluCI	AATT	4 cut(s)	162, 407, 508, 571
MluNI	TGGCCA	2 cut(s)	264, 355
MnlI	CCTC	6 cut(s)	291, 450, 567, 637, 892, 895
Mox20I	TGGCCA	2 cut(s)	264, 355
Mph1103I	ATGCAT	2 cut(s)	79, 506
MscI	TGGCCA	2 cut(s)	264, 355
MseI	TTAA	3 cut(s)	507, 845, 946
MslI	CAYNNNNRTG	1 cut(s)	594
Msp20I	TGGCCA	2 cut(s)	264, 355
MspA1I	CMGCKG	1 cut(s)	126
MspI	CCGG	1 cut(s)	907
MspR9I	CCNGG	2 cut(s)	66, 907
Mva1269I	GAATGC	1 cut(s)	300
MvaI	CCWGG	1 cut(s)	66
NciI	CCSGG	1 cut(s)	907
NdeII	GATC	4 cut(s)	202, 307, 412, 615
NlaIII	CATG	6 cut(s)	81, 93, 110, 352, 700, 705
NlaIV	GGNNCC	2 cut(s)	30, 813
NsiI	ATGCAT	2 cut(s)	79, 506
NspI	RCATGY	2 cut(s)	93, 110
PaeI	GCATGC	2 cut(s)	93, 110
PceI	AGGCCT	1 cut(s)	244
PctI	GAATGC	1 cut(s)	300
PfeI	GAWTC	3 cut(s)	467, 553, 919
PkrI	GCNGC	2 cut(s)	128, 649
PshBI	ATTAAT	1 cut(s)	507
PsiI	TTATAA	1 cut(s)	513
Psp124BI	GAGCTC	1 cut(s)	331
Psp6I	CCWGG	1 cut(s)	64
PspGI	CCWGG	1 cut(s)	64
PspN4I	GGNNCC	2 cut(s)	30, 813
PspPI	GGNCC	1 cut(s)	904
PstI	CTGCAG	1 cut(s)	615
PsuI	RGATCY	1 cut(s)	307
PvuII	CAGCTG	1 cut(s)	126
RsaI	GTAC	2 cut(s)	122, 813
RsaNI	GTAC	2 cut(s)	121, 812
RseI	CAYNNNNRTG	1 cut(s)	594
SacI	GAGCTC	1 cut(s)	331
SaqAI	TTAA	3 cut(s)	507, 845, 946
SatI	GCNGC	2 cut(s)	127, 648
Sau3AI	GATC	4 cut(s)	202, 307, 412, 615
Sau96I	GGNCC	1 cut(s)	904
ScrFI	CCNGG	2 cut(s)	66, 907
SduI	GDGCHC	1 cut(s)	331
SfaNI	GCATC	7 cut(s)	64, 323, 430, 488, 491, 692, 742
SfcI	CTRYAG	1 cut(s)	611
SmiMI	CAYNNNNRTG	1 cut(s)	594
SphI	GCATGC	2 cut(s)	93, 110
Sse9I	AATT	4 cut(s)	162, 407, 508, 571
SseBI	AGGCCT	1 cut(s)	244
SsiI	CCGC	1 cut(s)	723
SspMI	CTAG	2 cut(s)	113, 674
SstI	GAGCTC	1 cut(s)	331
StuI	AGGCCT	1 cut(s)	244
StyD4I	CCNGG	2 cut(s)	64, 905
StyI	CCWWGG	1 cut(s)	294
TaaI	ACNGT	2 cut(s)	371, 938
TaqI	TCGA	2 cut(s)	8, 870
TaqII	GACCGA	1 cut(s)	729
TasI	AATT	4 cut(s)	162, 407, 508, 571
TatI	WGTACW	1 cut(s)	120
TfiI	GAWTC	3 cut(s)	467, 553, 919
Tru1I	TTAA	3 cut(s)	507, 845, 946
Tru9I	TTAA	3 cut(s)	507, 845, 946
TscAI	CASTG	2 cut(s)	241, 777
TseI	GCWGC	2 cut(s)	126, 647
TspDTI	ATGAA	4 cut(s)	66, 371, 654, 685
TspGWI	ACGGA	1 cut(s)	578
TspRI	CASTG	2 cut(s)	241, 777
VspI	ATTAAT	1 cut(s)	507
XapI	RAATTY	2 cut(s)	162, 571
XceI	RCATGY	2 cut(s)	93, 110
XcmI	CCANNNNNNNNNTGG	1 cut(s)	257
XmiI	GTMKAC	1 cut(s)	39
XspI	CTAG	2 cut(s)	113, 674
Zsp2I	ATGCAT	2 cut(s)	79, 506

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.