Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0005272

Physical Location & Seq

Genomic Coordinates Reverse (-)

24162732 .. 24164435

Gene Structure 1704 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1200100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 939 bp

ATGGCGTCAGAAACTCAGCCTAAGCTTCCTGTTATAGATTTATCTGATGAGAACTTGAAGCCCGGTACAGATGCATGGCTATTGGCATGCAAAGAAATCAAGGATGCACTAGAAGAGTATGGCTGCTTCGAAGCTATTTACCATAAAGTTCCTCTGGAACTTCACAACTCCATGTTTACTGCGATGGAAGATCTATTTGGTCTTCCATTAGAAACTAAAAAGCAAAAGACCAGCGATAGGCATTTACACAGCTATCTTGGACAATATTCATTCATTCCTCTCTATGAATCCTTGGCGATGGCTAATCCAACAACTGTTGAAGGAGCTCAAGGTTTCACAAGCACCATGTGGCCTCAAGGAAATGACCATTTTTGTGAAACTGCTCATTCCTTCTCAAAGCTGGTAGCAGAATTGAGTCAAATGGTGACAAGAATGGTTTTTGATATGTACGGTGTGCAGAGGCTTTATGACTCTCACATGGAATTAACTACATACCAGCTTAGATGGTATAAGTACAGAACACAACAAGCGAATGAAACCAATGTGGGAATGCTTCCTCACACAGACAAGTCCTTCATATCCATACTGAATCAAGATCAGGTGAATGGTTTGCAGATTGGAACAAAGGATAGTCAGTGGATTGATGTGAAGCCTTCACCTTCATCTTTTCTAATCTTGGCAGGTGATGCATTCATGGCATGGAGCAATGATAGAGTACCCTCTTGTGAACATCAAGTCACCATGAGAGAGAACAAAACCAGATACTCCCTAGGTCTGTTCTCATTCAAAAGTGGGATTATACAAGTACCTGAAGAGCTAATTGATGAGAAACATCCCTTATTGTATAAGCCATTTGATCATTTCAGTTTCCTTGACTTCGGTCGAACACCAGAGGCAAGGAATTTGTTGCGTTCTATCAAAGCCTACTGTGGTGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0006082 GO:0006629 GO:0006720 GO:0006721 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008299 GO:0008610 GO:0009058 GO:0009314 GO:0009416 GO:0009628 GO:0009685 GO:0009686 GO:0009719 GO:0009725 GO:0009739 GO:0009740 GO:0009755 GO:0009987 GO:0010033 GO:0010476 GO:0010817 GO:0016053 GO:0016101 GO:0016102 GO:0016114 GO:0016491 GO:0016705 GO:0016706 GO:0016707 GO:0019752 GO:0023052 GO:0032870 GO:0033993 GO:0042221 GO:0042445 GO:0042446 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044283 GO:0046394 GO:0050789 GO:0050794 GO:0050896 GO:0051213 GO:0051716 GO:0055114 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071370 GO:0071396 GO:0071495 GO:0071704 GO:1901576 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

313

Amino Acids

35.75

Weight (kDa)

5.56

Isoelectric Point (pI)

34.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	9 - 85	2.4e-12	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	170 - 261	7.4e-24	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005272)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G52800
fragaria_vesca	FvH4_6g30750
malus_domestica	MD03G1070400.v1.1 MD11G1074400.v1.1 MD17G1199500.v1.1 MD17G1200100.v1.1 MD17G1200500.v1.1
prunus_persica	Prupe.3G076200_v2.0.a1
pyrus_communis	pycom03g05650 pycom03g05660 pycom15g25880 pycom15g25950
rosa_chinensis	RchiOBHm_Chr2g0137181 RchiOBHm_Chr5g0080031
rosa_laevigata	RLG00000019632 RLG00000036847
rosa_multiflora	Rmu_sc0000894.1_g000045
rosa_roxburghii	Rroxscaffold_1G00003110 Rroxscaffold_2G00107410
rosa_rugosa	Rorug05G0467900
rosa_samantha	Rh2AG388000 Rh2BG395800 Rh2CG375300 Rh2DG410800 Rh5AG521400 Rh5CG566900 Rh5DG553500
rosa_wichuraiana	Rw2G031800 Rw5G048390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	671
Acc36I	ACCTGC	1 cut(s)	671
AcsI	RAATTY	1 cut(s)	901
AcuI	CTGAAG	1 cut(s)	831
AcyI	GRCGYC	1 cut(s)	5
AdeI	CACNNNGTG	1 cut(s)	348
AfaI	GTAC	5 cut(s)	67, 449, 515, 717, 807
AfiI	CCNNNNNNNGG	1 cut(s)	237
AgsI	TTSAA	3 cut(s)	58, 320, 787
AjuI	GAANNNNNNNTTGG	2 cut(s)	180, 212
AluBI	AGCT	7 cut(s)	25, 134, 252, 326, 400, 499, 817
AluI	AGCT	7 cut(s)	25, 134, 252, 326, 400, 499, 817
Alw21I	GWGCWC	1 cut(s)	328
AoxI	GGCC	1 cut(s)	350
ApeKI	GCWGC	1 cut(s)	123
ApoI	RAATTY	1 cut(s)	901
AspA2I	CCTAGG	1 cut(s)	769
AsuC2I	CCSGG	1 cut(s)	63
AsuHPI	GGTGA	5 cut(s)	436, 613, 648, 695, 730
AsuII	TTCGAA	1 cut(s)	129
AvrII	CCTAGG	1 cut(s)	769
BanII	GRGCYC	1 cut(s)	328
BarI	GAAGNNNNNNTAC	2 cut(s)	110, 142
BbsI	GAAGAC	1 cut(s)	194
Bbv12I	GWGCWC	1 cut(s)	328
BbvI	GCAGC	1 cut(s)	110
BccI	CCATC	3 cut(s)	178, 292, 498
BclI	TGATCA	1 cut(s)	856
BcnI	CCSGG	1 cut(s)	63
BfaI	CTAG	2 cut(s)	110, 770
BfuAI	ACCTGC	1 cut(s)	671
BglII	AGATCT	1 cut(s)	190
BisI	GCNGC	1 cut(s)	124
BlnI	CCTAGG	1 cut(s)	769
BlsI	GCNGC	1 cut(s)	125
Bme1390I	CCNGG	1 cut(s)	63
BmrFI	CCNGG	1 cut(s)	63
BmsI	GCATC	3 cut(s)	61, 94, 676
BoxI	GACNNNNGTC	1 cut(s)	879
BpiI	GAAGAC	1 cut(s)	194
Bpu10I	CCTNAGC	1 cut(s)	21
Bpu14I	TTCGAA	1 cut(s)	129
BpuEI	CTTGAG	2 cut(s)	312, 339
BpuMI	CCSGG	1 cut(s)	63
BsaHI	GRCGYC	1 cut(s)	5
BsaJI	CCNNGG	2 cut(s)	291, 769
Bsc4I	CCNNNNNNNGG	1 cut(s)	237
Bse3DI	GCAATG	1 cut(s)	712
BseDI	CCNNGG	2 cut(s)	291, 769
BseGI	GGATG	2 cut(s)	109, 832
BseLI	CCNNNNNNNGG	1 cut(s)	237
BseMI	GCAATG	1 cut(s)	712
BseMII	CTCAG	1 cut(s)	29
BseXI	GCAGC	1 cut(s)	110
BsgI	GTGCAG	1 cut(s)	476
Bsh1285I	CGRYCG	1 cut(s)	883
BshFI	GGCC	1 cut(s)	352
BsiEI	CGRYCG	1 cut(s)	883
BsiHKAI	GWGCWC	1 cut(s)	328
BsiSI	CCGG	1 cut(s)	63
BslI	CCNNNNNNNGG	1 cut(s)	237
BsmI	GAATGC	2 cut(s)	555, 689
BsnI	GGCC	1 cut(s)	352
Bsp119I	TTCGAA	1 cut(s)	129
Bsp1286I	GDGCHC	1 cut(s)	328
Bsp143I	GATC	3 cut(s)	190, 595, 856
BspANI	GGCC	1 cut(s)	352
BspCNI	CTCAG	1 cut(s)	28
BspMI	ACCTGC	1 cut(s)	671
BspQI	GCTCTTC	1 cut(s)	807
BspT104I	TTCGAA	1 cut(s)	129
BsrDI	GCAATG	1 cut(s)	712
BssECI	CCNNGG	2 cut(s)	291, 769
BssMI	GATC	3 cut(s)	190, 595, 856
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	2 cut(s)	291, 769
Bst4CI	ACNGT	3 cut(s)	316, 452, 929
Bst6I	CTCTTC	2 cut(s)	108, 807
BstACI	GRCGYC	1 cut(s)	5
BstAPI	GCANNNNNTGC	1 cut(s)	686
BstBI	TTCGAA	1 cut(s)	129
BstC8I	GCNNGC	1 cut(s)	88
BstDEI	CTNAG	3 cut(s)	15, 21, 500
BstF5I	GGATG	2 cut(s)	109, 832
BstKTI	GATC	3 cut(s)	193, 598, 859
BstMBI	GATC	3 cut(s)	190, 595, 856
BstMCI	CGRYCG	1 cut(s)	883
BstMWI	GCNNNNNNNGC	2 cut(s)	686, 695
BstNSI	RCATGY	1 cut(s)	90
BstPAI	GACNNNNGTC	1 cut(s)	879
BstSCI	CCNGG	1 cut(s)	61
BstV1I	GCAGC	1 cut(s)	110
BstV2I	GAAGAC	1 cut(s)	194
BstX2I	RGATCY	1 cut(s)	190
BstYI	RGATCY	1 cut(s)	190
BsuRI	GGCC	1 cut(s)	352
BtgZI	GCGATG	2 cut(s)	197, 311
BtsCI	GGATG	2 cut(s)	109, 832
BtsIMutI	CAGTG	1 cut(s)	641
BveI	ACCTGC	1 cut(s)	671
Cac8I	GCNNGC	1 cut(s)	88
Csp6I	GTAC	5 cut(s)	66, 448, 514, 716, 806
CviAII	CATG	8 cut(s)	75, 87, 172, 346, 478, 694, 699, 742
CviQI	GTAC	5 cut(s)	66, 448, 514, 716, 806
DdeI	CTNAG	3 cut(s)	15, 21, 500
DpnI	GATC	3 cut(s)	192, 597, 858
DpnII	GATC	3 cut(s)	190, 595, 856
DraIII	CACNNNGTG	1 cut(s)	348
Eam1104I	CTCTTC	2 cut(s)	108, 807
EarI	CTCTTC	2 cut(s)	108, 807
Ecl136II	GAGCTC	1 cut(s)	326
Eco130I	CCWWGG	2 cut(s)	291, 769
Eco24I	GRGCYC	1 cut(s)	328
Eco53kI	GAGCTC	1 cut(s)	326
Eco57I	CTGAAG	1 cut(s)	831
EcoICRI	GAGCTC	1 cut(s)	326
EcoT14I	CCWWGG	2 cut(s)	291, 769
EcoT22I	ATGCAT	2 cut(s)	76, 691
EcoT38I	GRGCYC	1 cut(s)	328
ErhI	CCWWGG	2 cut(s)	291, 769
FaeI	CATG	8 cut(s)	78, 90, 175, 349, 481, 697, 702, 745
FatI	CATG	8 cut(s)	74, 86, 171, 345, 477, 693, 698, 741
FbaI	TGATCA	1 cut(s)	856
Fnu4HI	GCNGC	1 cut(s)	124
FokI	GGATG	2 cut(s)	116, 819
FriOI	GRGCYC	1 cut(s)	328
Fsp4HI	GCNGC	1 cut(s)	124
FspBI	CTAG	2 cut(s)	110, 770
GluI	GCNGC	1 cut(s)	124
HaeIII	GGCC	1 cut(s)	352
HapII	CCGG	1 cut(s)	63
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	8 cut(s)	78, 90, 175, 349, 481, 697, 702, 745
HindIII	AAGCTT	1 cut(s)	23
HinfI	GANTC	4 cut(s)	287, 415, 470, 589
HpaII	CCGG	1 cut(s)	63
HphI	GGTGA	5 cut(s)	436, 613, 648, 695, 730
Hpy166II	GTNNAC	2 cut(s)	177, 728
Hpy188I	TCNGA	2 cut(s)	10, 46
Hpy188III	TCNNGA	2 cut(s)	155, 593
Hpy8I	GTNNAC	2 cut(s)	177, 728
HpyAV	CCTTC	5 cut(s)	314, 400, 583, 663, 669
HpyCH4III	ACNGT	3 cut(s)	316, 452, 929
HpyCH4V	TGCA	6 cut(s)	74, 90, 107, 457, 613, 689
HpyF10VI	GCNNNNNNNGC	2 cut(s)	686, 695
HpyF3I	CTNAG	3 cut(s)	15, 21, 500
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	8 cut(s)	78, 90, 175, 349, 481, 697, 702, 745
Ksp22I	TGATCA	1 cut(s)	856
Kzo9I	GATC	3 cut(s)	190, 595, 856
LguI	GCTCTTC	1 cut(s)	807
LmnI	GCTCC	2 cut(s)	323, 702
Lsp1109I	GCAGC	1 cut(s)	110
LweI	GCATC	3 cut(s)	61, 94, 676
MaeI	CTAG	2 cut(s)	110, 770
MaeIII	GTNAC	2 cut(s)	424, 736
MalI	GATC	3 cut(s)	192, 597, 858
MboI	GATC	3 cut(s)	190, 595, 856
MboII	GAAGA	4 cut(s)	125, 194, 200, 824
MflI	RGATCY	1 cut(s)	190
MhlI	GDGCHC	1 cut(s)	328
MluCI	AATT	4 cut(s)	410, 482, 819, 901
MlyI	GAGTC	2 cut(s)	424, 464
MmeI	TCCRAC	1 cut(s)	332
MnlI	CCTC	7 cut(s)	162, 288, 363, 453, 567, 730, 886
Mph1103I	ATGCAT	2 cut(s)	76, 691
MseI	TTAA	1 cut(s)	485
MslI	CAYNNNNRTG	1 cut(s)	372
MspI	CCGG	1 cut(s)	63
MspR9I	CCNGG	1 cut(s)	63
Mva1269I	GAATGC	2 cut(s)	555, 689
MwoI	GCNNNNNNNGC	2 cut(s)	686, 695
NciI	CCSGG	1 cut(s)	63
NdeII	GATC	3 cut(s)	190, 595, 856
NlaIII	CATG	8 cut(s)	78, 90, 175, 349, 481, 697, 702, 745
NmuCI	GTSAC	2 cut(s)	424, 736
NsiI	ATGCAT	2 cut(s)	76, 691
NspI	RCATGY	1 cut(s)	90
NspV	TTCGAA	1 cut(s)	129
PaeI	GCATGC	1 cut(s)	90
PaqCI	CACCTGC	1 cut(s)	671
PciSI	GCTCTTC	1 cut(s)	807
PctI	GAATGC	2 cut(s)	555, 689
PfeI	GAWTC	2 cut(s)	287, 589
PkrI	GCNGC	1 cut(s)	125
PleI	GAGTC	2 cut(s)	423, 464
PpsI	GAGTC	2 cut(s)	423, 464
PshAI	GACNNNNGTC	1 cut(s)	879
Psp124BI	GAGCTC	1 cut(s)	328
PsuI	RGATCY	1 cut(s)	190
RsaI	GTAC	5 cut(s)	67, 449, 515, 717, 807
RsaNI	GTAC	5 cut(s)	66, 448, 514, 716, 806
RseI	CAYNNNNRTG	1 cut(s)	372
SacI	GAGCTC	1 cut(s)	328
SapI	GCTCTTC	1 cut(s)	807
SaqAI	TTAA	1 cut(s)	485
SatI	GCNGC	1 cut(s)	124
Sau3AI	GATC	3 cut(s)	190, 595, 856
SchI	GAGTC	2 cut(s)	424, 464
ScrFI	CCNGG	1 cut(s)	63
SduI	GDGCHC	1 cut(s)	328
SfaNI	GCATC	3 cut(s)	61, 94, 676
SfuI	TTCGAA	1 cut(s)	129
SmiMI	CAYNNNNRTG	1 cut(s)	372
SmlI	CTYRAG	2 cut(s)	327, 354
SmoI	CTYRAG	2 cut(s)	327, 354
SphI	GCATGC	1 cut(s)	90
Sse9I	AATT	4 cut(s)	410, 482, 819, 901
SspI	AATATT	1 cut(s)	266
SspMI	CTAG	2 cut(s)	110, 770
SstI	GAGCTC	1 cut(s)	328
StyD4I	CCNGG	1 cut(s)	61
StyI	CCWWGG	2 cut(s)	291, 769
TaaI	ACNGT	3 cut(s)	316, 452, 929
TaqI	TCGA	2 cut(s)	129, 883
TaqII	GACCGA	1 cut(s)	869
TasI	AATT	4 cut(s)	410, 482, 819, 901
TatI	WGTACW	1 cut(s)	513
TfiI	GAWTC	2 cut(s)	287, 589
Tru1I	TTAA	1 cut(s)	485
Tru9I	TTAA	1 cut(s)	485
TscAI	CASTG	1 cut(s)	641
TseFI	GTSAC	2 cut(s)	424, 736
TseI	GCWGC	1 cut(s)	123
Tsp45I	GTSAC	2 cut(s)	424, 736
TspDTI	ATGAA	7 cut(s)	258, 262, 300, 549, 565, 651, 682
TspRI	CASTG	1 cut(s)	641
XapI	RAATTY	1 cut(s)	901
XceI	RCATGY	1 cut(s)	90
XmaJI	CCTAGG	1 cut(s)	769
XspI	CTAG	2 cut(s)	110, 770
Zsp2I	ATGCAT	2 cut(s)	76, 691

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.