Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0005110

Physical Location & Seq

Genomic Coordinates Forward (+)

6311415 .. 6324386

Gene Structure 12972 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1077900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4065 bp

ATGGATCCCGTAGAAGCCGCGAACGGGCTCTCGTTCCGGGTCGGGTTCTCCGGCCACAGTGGCCACCTCCGGCTCGAGCCCCTCTCCACTGTCGAGAGCTCCGACCCCGTCAAGTCTCTCCCTGATTTCATTCTGCCGCCTGCATTTCCTAGGGAGACGCCCGAATCAATAAAAGAGTATATAGAGGAGAAGTATCTTTCGCCAAGATTGGACGACGAAGTATTTGCACCCGAAAAAGTTGGGAGGCAGTGGGATTTCGACTGGTTTGATAAGGCAAATGTACCCTTGGAGCCAACACTGCCGCGCTGCGTTGTGGTTCCCACGTGGGAATTGCCGTTTCGGCGCCAGAAGAGTGGGTCGGCCGAATGTCAATGGGAACCTAAATCCGTGGAGGTGGATGTCTCAGAACTTATAGTAGGAGCTCAAGAGTCTGGTTCATTGCCACGTTTGGCTGGACCAGCGAAGGATTTTGTACGGGGAAGCATCAACAACCGCCCTTTTCGTCCGGGAGGCTTGGATGATTCCCAATCTCTAGAAAGAGTTCTTCCTGATGGTGCTTCTAATGGCGAATGGGTACACGAACTTCTTGTTGGTGGCTCTCCTCAGGCTGTACCTCCAAGCTTTAAGCAAGGAATGGACCTTGGTGATCTGAAGGCCTATCCATGCTCGTGGAATGTATATAAGGATCAAAGTTCCCTGGAGAGCACATCAGATGAAAAACTGTCTTTGCAGAGTGGGTTGTCTGTACAGTTTGATGACCTGTTCAAGAAAGCTTGGGAAGAGGATGTTGTTGAATTTGAAGGAGATGGTCAATTGTCAGGATCAGAATCTGTTAAGTCAGAGGATGAAGCAAAGGAGGTTGATGTTGCCATCGATACTTCTGAGCCTGAATTGTCTTTGTTGGATGAGATTTTGTCAGTTGCGGCAGGAGAATCAAAGTCAAGATCTAATGGAATTGGTGAAGATGGTGAACAAAATCCGAAGAAGGCTTGGGCTATTAGTGGAGGTACTGAATATATTGCAGAGAACTTTCATGATCTTGTTCCCGACAAAGCACTTGATTTTCCGTTTGAATTGGACAAATTCCAGAAGGAGGCTATTTATTATCTTGAAAAGGGGGACTCAGTATTTGTGGCTGCTCACACATCAGCTGGAAAGACAGTTGTTGCAGAATATGCATTTGCTTTAGCATCAAAACATTGTACCAGAGCTGTGTATACTGCTCCTATCAAAACCATCAGCAATCAAAAATATAGAGATTTGTGTGGAAAATTTGATGTTGGACTTCTCACTGGTGATGTTAGCTTGAGGCCAGAGGCTTCTTGTCTTATTATGACCACCGAAATATTAAGGTCAATGCTTTATCGTGGTGCTGACATAATACGTGATATTGAATGGGTTATCTTTGATGAAGTGCACTATGTCAATGATGTTGAAAGAGGTGTTGTTTGGGAAGAAGTTATTATAATGCTACCAAGACACATCAATATTGTTCTCCTTTCAGCTACGGTACCAAACAAAGTTGAGTTTGCTGACTGGATTGGACAGACAAAGCAAAAGCAAATTCGTGTTACTGGGACTACAAAAAGACCAGTTCCATTGGAGCACTGTCTATTTTACTCCGGAGAACTTTACAAAATATGTGAAAGCGAAATCTTCATTCCCCAGGGGTTCAAAGCTGCAAAAGATGCATTTAAGAAAAAGAATATGAGTGCCACTACTGGTGGTAGTGGATCACAGGCACCAGCTTCAGCTTCTCATGATGGGACTCGATCTCAAAAACAAAGTTCAAACTGGGGCGGAAAACAGAAGAAGCAATCTGGTTCCCAAAATTCAGGGAATTTCTCCCGAACTGGAGGAGCAAATCAAAACAATGGAAATGGCATGAACAGTTGGGGTTTAAGAAGATCAGATGCTTCTTTGTGGTTGTCACTTATTAACAAGCTCTCAAAGATGTCACTATTACCTGTGGTTGTATTTTGTTTCTCAAAGATTCGCTGTGATAAGTCAGCTGATAGTATGTATGGGACTGACCTTACAAGTAGTTCTGAGAAAAGTGAGATTCGTGTTTTCTGTGACAAAGCATTTTCGCGCCTAAAGGGATCTGACAGGACTTTACCACAGGTTGTCAGAGTTCAAAGCCTTCTCCATAGAGGAATTGGCGTCCATCATGCTGGACTACTTCCAATTGTTAAGGAAGTAGTGGAAATGCTTTTTTGTCGCGGAGTGATCAAGGTTTTGTTCTCAACAGAGACATTTGCAATGGGGGTTAATGCACCAGCTAGAACGGTTGTTTTTGATACGTTAAGGAAATTTGATGGCAAGGAATTTAGACAATTATTTCCAGGAGAATACACTCAAATGGCAGGCCGTGCAGGCAGAAGAGGACTTGATAAAATTGGTACAGTTATTGTAATGTGCCGTGATGAAATCCTAGAAGAGGGTGATTTGAAGAATGTCATAGTTGGAAGTGCAACCAAGCTTGAATCTCAGTTTCGGCTTACCTATATCATGATCCTGCATCTCCTTCGTGTTGAGGAACTGAAGGTGGAGGACATGCTGAAAAGAAGTTTTGCTGAGTTCCATGCTCAGAAGAAACTACCAGAACATCAGCGACTTCTGATGCGAAAGCTTGCTCAGCCTACAAAAACTATTGAATGTGTAAAATGTGAAACAGCTATTGAGGAGTATTATGACATGTACTCAGAAGCTGCGAAGTATAGCAAGGAAATATTGGAGGCCGTTATGCAGTCTCCTATTGCCAAACAATTTCTTACAACTGGGAGAGTGGTAGTCATGGGATCACACTCAGCACAGGACCACTTGCTTGGAGTCGTCGTGAAAGCACCTAGTGCTAATAATAAACAGTACATCGTTTTGGTGCTGAAACCTGAATTATTGCCCCAGACTCCACTGGGTAGTGGTAACTTGCAAGACAGTAAAAACACAGATTTTCCACAAGGTTATTTTGTGGTACCAAAATCTAAACGTGCTCTTGATGAAGAGTATCGTTCTGGTGTCACACCTCGGAAAGTATCGGGTGTCATCAAGATAAAATTACCACACCAGGGTTCTGCTGCTGGGGTGAGCTTTGAGGTTAAAGAAGTTGACAGCAAAGATTTTCTATGCATATGCAATTGCAAGATAAAAATTGACCAAGTTGGGCTTCTTGAAGACAATAGCAATGCTGCTTACTCCAAGACAGTTCAACAGCTGTTGGACACAAAATCTGACAGAAATGAGTACCCTCCAGCCTTGGATCCGTATAAAGATCTGAATTTGAGAGACGTAAATCTTGTCGGAACATACTATAAGTGGATTAACTTATTGCAAAAGATGGCAACGAACAAGTGCCATGGGTGTATAAAGTTGGACGAGCACATTAAGTTAGCAAGAGAGATAAAGAGACACAGTGAGGAAGTCGATGCTCTTAAGTTTGAAATGTCAGATGAAGCACTTAAACAAATGCCAGACTTTCAAGGCCGGATAGATGTTCTGAAGGAAATTGGATGTATAGATGCTGATCTTGTGGTTCAAATAAAAGGCCGTGTTGCATGCGAAATGAATTCTGGGGAGGAGTTGATATCCACAGAGTGTTTGTTTGAAAACCAACTGGATGACCTGGAACCAGAAGAAGCAGTGGCGTTGATGTCTGCCTTTGTGTTTCAGCAGAGGAAGACTTCAGATCCTTCTCTTACTCCAAAACTGTCTCAGGCCAAACAGAGGTTGTACGATACAGCAATAAGACTTGGCCAGCTTCAAGCCCACTACAATGTTCAAATAAATCCAGAGGAGTATGCTCGAGAGAATCTCAAGTTTGGTCTTGTTGAAGTCGTTTATGAGTGGGCAAAGGGCACTCCGTTCGCAGACATTTGCGAACTTACAGATGTTCCAGAAGGCATGATAGTTCGAACCATTGTCAGACTTGATGAGACGTGCCGTGAGTTCAAAAATGCTGCATCTATTATGGGTAATTCTGCTCTGTACAAGAAAATGGAAACAGCTTCAAATGCGATAAAGCGTGACATTGTATTTGCCGCTAGCTTATACGTCACCGGAGTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000184 GO:0000288 GO:0000291 GO:0000956 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010035 GO:0010038 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0015672 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0019439 GO:0030001 GO:0032991 GO:0034427 GO:0034641 GO:0034655 GO:0035864 GO:0040029 GO:0042221 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0050789 GO:0050896 GO:0051179 GO:0051234 GO:0055087 GO:0060255 GO:0065007 GO:0070035 GO:0070478 GO:0071704 GO:0090304 GO:0140098 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

DEAD DSHCT Helicase_C rRNA_proc-arch

Protein Analysis

1355

Amino Acids

150.91

Weight (kDa)

5.62

Isoelectric Point (pI)

42.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ski2_N	PF17911	141 - 216	9.2e-08	Ski2 N-terminal region
DEAD	PF00270	361 - 509	9.4e-23	DEAD/DEAH box helicase
MTR4-like_stalk	PF21408	851 - 915	1.7e-13	Exosome RNA helicase MTR4-like, stalk
MTR4_beta-barrel	PF13234	918 - 1150	7.3e-26	Mtr4-like, beta-barrel domain
DSHCT	PF08148	1176 - 1346	9.8e-59	DSHCT (NUC185) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005110)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46960 AT3G46960 AT3G46960
fragaria_vesca	FvH4_3g37880 FvH4_3g37880 FvH4_3g37880 FvH4_3g37880 FvH4_3g37880
malus_domestica	MD03G1077900.v1.1 MD11G1082500.v1.1
prunus_persica	Prupe.6G062900_v2.0.a1 Prupe.6G062900_v2.0.a1 Prupe.6G062900_v2.0.a1
pyrus_communis	pycom03g06210 pycom11g07060
rosa_chinensis	RchiOBHm_Chr5g0068091
rosa_laevigata	RLG00000035954
rosa_multiflora	Rmu_sc0000435.1_g000017
rosa_roxburghii	Rroxscaffold_1G00012520 Rroxscaffold_1G00013260
rosa_rugosa	Rorug05G0387900 Rorug05G0388300 Rorug05G0388400 Rorug05G0388500
rosa_samantha	Rh5AG446500 Rh5BG464600 Rh5CG486400 Rh5DG478400
rosa_wichuraiana	Rw5G041700 Rw5G041740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1466
Acc65I	GGTACC	2 cut(s)	1510, 2974
AccB1I	GGYRCC	4 cut(s)	342, 1510, 1741, 2974
AccI	GTMKAC	1 cut(s)	1217
AccII	CGCG	4 cut(s)	20, 304, 2092, 2223
AccIII	TCCGGA	1 cut(s)	1622
AciI	CCGC	8 cut(s)	18, 137, 302, 493, 923, 1800, 2223, 4038
AcoI	YGGCCR	4 cut(s)	52, 61, 360, 3751
AcuI	CTGAAG	5 cut(s)	671, 1734, 2564, 3518, 3666
AcvI	CACGTG	1 cut(s)	324
AcyI	GRCGYC	3 cut(s)	158, 343, 2163
AdeI	CACNNNGTG	2 cut(s)	2852, 3594
AfiI	CCNNNNNNNGG	5 cut(s)	24, 1093, 2440, 3068, 3723
AflII	CTTAAG	1 cut(s)	3431
AflIII	ACRYGT	1 cut(s)	2697
AjiI	CACGTC	1 cut(s)	3936
AjnI	CCWGG	5 cut(s)	696, 1665, 2344, 3066, 3621
AjuI	GAANNNNNNNTTGG	4 cut(s)	573, 605, 3150, 3182
Alw21I	GWGCWC	7 cut(s)	101, 424, 707, 1419, 1608, 2995, 3381
Alw26I	GTCTC	9 cut(s)	120, 149, 406, 2246, 2757, 3279, 3400, 3714, 3926
Alw44I	GTGCAC	1 cut(s)	1415
AlwNI	CAGNNNCTG	2 cut(s)	830, 2711
Ama87I	CYCGRG	2 cut(s)	74, 3801
Aor13HI	TCCGGA	1 cut(s)	1622
ApaLI	GTGCAC	1 cut(s)	1415
ApeKI	GCWGC	7 cut(s)	306, 1136, 1679, 2711, 3077, 3188, 3956
ArsI	GACNNNNNNTTYG	6 cut(s)	206, 238, 1112, 1144, 4016, 4048
Asp700I	GAANNNNTTC	1 cut(s)	540
Asp718I	GGTACC	2 cut(s)	1510, 2974
AspA2I	CCTAGG	1 cut(s)	149
AspLEI	GCGC	3 cut(s)	306, 345, 2094
AspS9I	GGNCC	3 cut(s)	455, 637, 2818
AsuC2I	CCSGG	2 cut(s)	38, 507
AsuHPI	GGTGA	7 cut(s)	656, 971, 980, 1307, 2456, 3097, 4045
AsuII	TTCGAA	1 cut(s)	3910
AsuNHI	GCTAGC	1 cut(s)	4040
AvaI	CYCGRG	2 cut(s)	74, 3801
AvaII	GGWCC	3 cut(s)	455, 637, 2818
AvrII	CCTAGG	1 cut(s)	149
AxyI	CCTNAGG	1 cut(s)	603
BaeGI	GKGCMC	2 cut(s)	1419, 3857
BalI	TGGCCA	2 cut(s)	63, 3753
BamHI	GGATCC	2 cut(s)	4, 3259
BanI	GGYRCC	4 cut(s)	342, 1510, 1741, 2974
BanII	GRGCYC	4 cut(s)	30, 81, 101, 424
BarI	GAAGNNNNNNTAC	2 cut(s)	567, 599
BauI	CACGAG	1 cut(s)	667
BbrPI	CACGTG	1 cut(s)	324
BbsI	GAAGAC	2 cut(s)	3180, 3683
Bbv12I	GWGCWC	7 cut(s)	101, 424, 707, 1419, 1608, 2995, 3381
BbvI	GCAGC	7 cut(s)	293, 1123, 1666, 2698, 3064, 3175, 3943
BccI	CCATC	9 cut(s)	545, 800, 878, 959, 1244, 1757, 2175, 2312, 3331
BceAI	ACGGC	6 cut(s)	319, 2355, 2406, 2726, 3531, 3924
BcgI	CGANNNNNNTGC	6 cut(s)	206, 240, 2510, 2544, 3844, 3878
BciT130I	CCWGG	5 cut(s)	698, 1667, 2346, 3068, 3623
BclI	TGATCA	1 cut(s)	2229
BcnI	CCSGG	2 cut(s)	38, 507
BcoDI	GTCTC	9 cut(s)	120, 149, 406, 2246, 2757, 3279, 3400, 3714, 3926
BfaI	CTAG	6 cut(s)	150, 533, 2283, 2435, 2850, 4041
BfoI	RGCGCY	1 cut(s)	346
BfrI	CTTAAG	1 cut(s)	3431
BglI	GCCNNNNNGGC	3 cut(s)	60, 340, 2376
BglII	AGATCT	2 cut(s)	944, 3271
BlnI	CCTAGG	1 cut(s)	149
BlpI	GCTNAGC	1 cut(s)	2637
Bme1390I	CCNGG	7 cut(s)	38, 507, 698, 1667, 2346, 3068, 3623
Bme18I	GGWCC	3 cut(s)	455, 637, 2818
BmeT110I	CYCGRG	2 cut(s)	74, 3801
BmgBI	CACGTC	1 cut(s)	3936
BmgT120I	GGNCC	3 cut(s)	455, 637, 2818
BmrFI	CCNGG	7 cut(s)	38, 507, 698, 1667, 2346, 3068, 3623
BmrI	ACTGGG	4 cut(s)	1584, 1804, 2790, 2924
BmsI	GCATC	9 cut(s)	492, 1199, 1678, 1903, 2530, 2613, 3415, 3508, 3968
BmtI	GCTAGC	1 cut(s)	4044
BmuI	ACTGGG	4 cut(s)	1584, 1804, 2790, 2924
BpiI	GAAGAC	2 cut(s)	3180, 3683
BplI	GAGNNNNNCTC	4 cut(s)	68, 100, 3795, 3827
BpmI	CTGGAG	3 cut(s)	719, 1875, 3234
Bpu1102I	GCTNAGC	1 cut(s)	2637
Bpu14I	TTCGAA	1 cut(s)	3910
BpuEI	CTTGAG	3 cut(s)	408, 1327, 3797
BpuMI	CCSGG	2 cut(s)	38, 507
Bsa29I	ATCGAT	1 cut(s)	873
BsaAI	YACGTR	2 cut(s)	324, 1385
BsaBI	GATNNNNATC	2 cut(s)	826, 3522
BsaHI	GRCGYC	3 cut(s)	158, 343, 2163
BsaWI	WCCGGW	2 cut(s)	1622, 4055
BsaXI	ACNNNNNCTCC	2 cut(s)	2823, 2853
Bsc4I	CCNNNNNNNGG	5 cut(s)	24, 1093, 2440, 3068, 3723
Bse21I	CCTNAGG	1 cut(s)	603
Bse3DI	GCAATG	3 cut(s)	437, 2268, 3190
Bse8I	GATNNNNATC	2 cut(s)	826, 3522
BseAI	TCCGGA	1 cut(s)	1622
BseBI	CCWGG	5 cut(s)	698, 1667, 2346, 3068, 3623
BseCI	ATCGAT	1 cut(s)	873
BseGI	GGATG	7 cut(s)	403, 523, 790, 850, 910, 3515, 3622
BseJI	GATNNNNATC	2 cut(s)	826, 3522
BseLI	CCNNNNNNNGG	5 cut(s)	24, 1093, 2440, 3068, 3723
BseMI	GCAATG	3 cut(s)	437, 2268, 3190
BseRI	GAGGAG	6 cut(s)	200, 591, 1872, 2699, 3590, 3806
BseSI	GKGCMC	2 cut(s)	1419, 3857
BseX3I	CGGCCG	1 cut(s)	360
BseXI	GCAGC	7 cut(s)	293, 1123, 1666, 2698, 3064, 3175, 3943
BseYI	CCCAGC	1 cut(s)	3080
BsgI	GTGCAG	1 cut(s)	2394
Bsh1236I	CGCG	4 cut(s)	20, 304, 2092, 2223
Bsh1285I	CGRYCG	1 cut(s)	363
BshNI	GGYRCC	4 cut(s)	342, 1510, 1741, 2974
BshVI	ATCGAT	1 cut(s)	873
BsiEI	CGRYCG	1 cut(s)	363
BsiHKAI	GWGCWC	7 cut(s)	101, 424, 707, 1419, 1608, 2995, 3381
BsiHKCI	CYCGRG	2 cut(s)	74, 3801
BsiSI	CCGG	7 cut(s)	37, 51, 70, 506, 1623, 3484, 4056
BslFI	GGGAC	4 cut(s)	1133, 1591, 1780, 2041
BslI	CCNNNNNNNGG	5 cut(s)	24, 1093, 2440, 3068, 3723
BsmAI	GTCTC	9 cut(s)	120, 149, 406, 2246, 2757, 3279, 3400, 3714, 3926
BsmBI	CGTCTC	3 cut(s)	149, 3279, 3926
BsmFI	GGGAC	4 cut(s)	1133, 1591, 1780, 2041
BsoBI	CYCGRG	2 cut(s)	74, 3801
Bsp119I	TTCGAA	1 cut(s)	3910
Bsp13I	TCCGGA	1 cut(s)	1622
Bsp1407I	TGTACA	2 cut(s)	745, 3984
Bsp1720I	GCTNAGC	1 cut(s)	2637
Bsp19I	CCATGG	1 cut(s)	3355
BspACI	CCGC	8 cut(s)	18, 137, 302, 493, 923, 1800, 2223, 4038
BspDI	ATCGAT	1 cut(s)	873
BspEI	TCCGGA	1 cut(s)	1622
BspFNI	CGCG	4 cut(s)	20, 304, 2092, 2223
BspHI	TCATGA	3 cut(s)	1033, 1759, 2511
BspOI	GCTAGC	1 cut(s)	4044
BspT104I	TTCGAA	1 cut(s)	3910
BspT107I	GGYRCC	4 cut(s)	342, 1510, 1741, 2974
BspTI	CTTAAG	1 cut(s)	3431
BsrDI	GCAATG	3 cut(s)	437, 2268, 3190
BsrGI	TGTACA	2 cut(s)	745, 3984
BssNAI	GTATAC	1 cut(s)	1218
BssNI	GRCGYC	3 cut(s)	158, 343, 2163
BssSI	CACGAG	1 cut(s)	667
BssT1I	CCWWGG	5 cut(s)	149, 285, 640, 3255, 3355
Bst1107I	GTATAC	1 cut(s)	1218
Bst2BI	CACGAG	1 cut(s)	667
Bst2UI	CCWGG	5 cut(s)	698, 1667, 2346, 3068, 3623
Bst6I	CTCTTC	5 cut(s)	344, 774, 2377, 2433, 2997
BstACI	GRCGYC	3 cut(s)	158, 343, 2163
BstAFI	CTTAAG	1 cut(s)	3431
BstAPI	GCANNNNNTGC	4 cut(s)	1175, 1688, 2372, 2852
BstAUI	TGTACA	2 cut(s)	745, 3984
BstBAI	YACGTR	2 cut(s)	324, 1385
BstBI	TTCGAA	1 cut(s)	3910
BstC8I	GCNNGC	7 cut(s)	141, 2368, 2377, 2634, 3556, 3755, 4042
BstDSI	CCRYGG	2 cut(s)	387, 3355
BstENI	CCTNNNNNAGG	1 cut(s)	2438
BstF5I	GGATG	7 cut(s)	403, 523, 790, 850, 910, 3515, 3622
BstFNI	CGCG	4 cut(s)	20, 304, 2092, 2223
BstH2I	RGCGCY	1 cut(s)	346
BstHHI	GCGC	3 cut(s)	306, 345, 2094
BstMAI	GTCTC	9 cut(s)	120, 149, 406, 2246, 2757, 3279, 3400, 3714, 3926
BstMCI	CGRYCG	1 cut(s)	363
BstNI	CCWGG	5 cut(s)	698, 1667, 2346, 3068, 3623
BstNSI	RCATGY	3 cut(s)	2560, 2701, 3558
BstSCI	CCNGG	7 cut(s)	36, 505, 696, 1665, 2344, 3066, 3621
BstSLI	GKGCMC	2 cut(s)	1419, 3857
BstUI	CGCG	4 cut(s)	20, 304, 2092, 2223
BstV1I	GCAGC	7 cut(s)	293, 1123, 1666, 2698, 3064, 3175, 3943
BstV2I	GAAGAC	2 cut(s)	3180, 3683
BstX2I	RGATCY	6 cut(s)	4, 944, 2102, 3259, 3271, 3685
BstXI	CCANNNNNNTGG	4 cut(s)	353, 669, 2174, 2828
BstYI	RGATCY	6 cut(s)	4, 944, 2102, 3259, 3271, 3685
BstZ17I	GTATAC	1 cut(s)	1218
BstZI	CGGCCG	1 cut(s)	360
Bsu15I	ATCGAT	1 cut(s)	873
Bsu36I	CCTNAGG	1 cut(s)	603
BsuTUI	ATCGAT	1 cut(s)	873
BtgI	CCRYGG	2 cut(s)	387, 3355
BtrI	CACGTC	1 cut(s)	3936
BtsCI	GGATG	7 cut(s)	403, 523, 790, 850, 910, 3515, 3622
BtsI	GCAGTG	3 cut(s)	254, 296, 3645
BtsIMutI	CAGTG	9 cut(s)	64, 87, 254, 296, 1290, 1606, 2912, 3418, 3645
Cac8I	GCNNGC	7 cut(s)	141, 2368, 2377, 2634, 3556, 3755, 4042
CaiI	CAGNNNCTG	2 cut(s)	830, 2711
CciI	TCATGA	3 cut(s)	1033, 1759, 2511
CfoI	GCGC	3 cut(s)	306, 345, 2094
Cfr13I	GGNCC	3 cut(s)	455, 637, 2818
ClaI	ATCGAT	1 cut(s)	873
CseI	GACGC	2 cut(s)	166, 2152
DinI	GGCGCC	1 cut(s)	344
DraIII	CACNNNGTG	2 cut(s)	2852, 3594
EaeI	YGGCCR	4 cut(s)	52, 61, 360, 3751
EagI	CGGCCG	1 cut(s)	360
Eam1104I	CTCTTC	5 cut(s)	344, 774, 2377, 2433, 2997
EarI	CTCTTC	5 cut(s)	344, 774, 2377, 2433, 2997
EciI	GGCGGA	1 cut(s)	1815
Ecl136II	GAGCTC	2 cut(s)	99, 422
EclXI	CGGCCG	1 cut(s)	360
Eco130I	CCWWGG	5 cut(s)	149, 285, 640, 3255, 3355
Eco147I	AGGCCT	1 cut(s)	656
Eco24I	GRGCYC	4 cut(s)	30, 81, 101, 424
Eco32I	GATATC	1 cut(s)	3585
Eco47I	GGWCC	3 cut(s)	455, 637, 2818
Eco52I	CGGCCG	1 cut(s)	360
Eco53kI	GAGCTC	2 cut(s)	99, 422
Eco57I	CTGAAG	5 cut(s)	671, 1734, 2564, 3518, 3666
Eco72I	CACGTG	1 cut(s)	324
Eco81I	CCTNAGG	1 cut(s)	603
Eco88I	CYCGRG	2 cut(s)	74, 3801
EcoICRI	GAGCTC	2 cut(s)	99, 422
EcoNI	CCTNNNNNAGG	1 cut(s)	2438
EcoRI	GAATTC	1 cut(s)	3565
EcoRII	CCWGG	5 cut(s)	696, 1665, 2344, 3066, 3621
EcoRV	GATATC	1 cut(s)	3585
EcoT14I	CCWWGG	5 cut(s)	149, 285, 640, 3255, 3355
EcoT22I	ATGCAT	3 cut(s)	1180, 1693, 3131
EcoT38I	GRGCYC	4 cut(s)	30, 81, 101, 424
EgeI	GGCGCC	1 cut(s)	344
EheI	GGCGCC	1 cut(s)	344
ErhI	CCWWGG	5 cut(s)	149, 285, 640, 3255, 3355
Esp3I	CGTCTC	3 cut(s)	149, 3279, 3926
FalI	AAGNNNNNCTT	2 cut(s)	3150, 3182
FaqI	GGGAC	4 cut(s)	1133, 1591, 1780, 2041
FauNDI	CATATG	1 cut(s)	3131
FbaI	TGATCA	1 cut(s)	2229
FblI	GTMKAC	1 cut(s)	1217
FokI	GGATG	7 cut(s)	410, 530, 797, 857, 917, 3522, 3629
FriOI	GRGCYC	4 cut(s)	30, 81, 101, 424
FspBI	CTAG	6 cut(s)	150, 533, 2283, 2435, 2850, 4041
GlaI	GCGC	3 cut(s)	305, 344, 2093
GsaI	CCCAGC	1 cut(s)	3084
GsuI	CTGGAG	3 cut(s)	719, 1875, 3234
HaeII	RGCGCY	1 cut(s)	346
HapII	CCGG	7 cut(s)	37, 51, 70, 506, 1623, 3484, 4056
HgaI	GACGC	2 cut(s)	166, 2152
HhaI	GCGC	3 cut(s)	306, 345, 2094
Hin1I	GRCGYC	3 cut(s)	158, 343, 2163
Hin6I	GCGC	3 cut(s)	304, 343, 2092
HinP1I	GCGC	3 cut(s)	304, 343, 2092
HincII	GTYRAC	1 cut(s)	3109
HindII	GTYRAC	1 cut(s)	3109
HindIII	AAGCTT	4 cut(s)	619, 771, 2480, 2630
HpaII	CCGG	7 cut(s)	37, 51, 70, 506, 1623, 3484, 4056
HphI	GGTGA	7 cut(s)	656, 971, 980, 1307, 2456, 3097, 4045
Hpy166II	GTNNAC	5 cut(s)	577, 971, 1218, 1417, 3109
Hpy8I	GTNNAC	5 cut(s)	577, 971, 1218, 1417, 3109
Hpy99I	CGWCG	2 cut(s)	218, 2840
HpyCH4IV	ACGT	8 cut(s)	323, 445, 1384, 2303, 2989, 3288, 3935, 4050
HpySE526I	ACGT	8 cut(s)	323, 445, 1384, 2303, 2989, 3288, 3935, 4050
Hsp92I	GRCGYC	3 cut(s)	158, 343, 2163
HspAI	GCGC	3 cut(s)	304, 343, 2092
KasI	GGCGCC	1 cut(s)	342
Kpn2I	TCCGGA	1 cut(s)	1622
KpnI	GGTACC	2 cut(s)	1514, 2978
Ksp22I	TGATCA	1 cut(s)	2229
LmnI	GCTCC	6 cut(s)	104, 289, 419, 1228, 1603, 1859
Lsp1109I	GCAGC	7 cut(s)	293, 1123, 1666, 2698, 3064, 3175, 3943
LweI	GCATC	9 cut(s)	492, 1199, 1678, 1903, 2530, 2613, 3415, 3508, 3968
MaeI	CTAG	6 cut(s)	150, 533, 2283, 2435, 2850, 4041
MaeII	ACGT	8 cut(s)	323, 445, 1384, 2303, 2989, 3288, 3935, 4050
MaeIII	GTNAC	8 cut(s)	1570, 1929, 1956, 2075, 2924, 3019, 4022, 4051
MfeI	CAATTG	3 cut(s)	812, 2187, 3136
MflI	RGATCY	6 cut(s)	4, 944, 2102, 3259, 3271, 3685
MlsI	TGGCCA	2 cut(s)	63, 3753
MluNI	TGGCCA	2 cut(s)	63, 3753
Mly113I	GGCGCC	1 cut(s)	343
MlyI	GAGTC	5 cut(s)	437, 1115, 1762, 2841, 2902
MmeI	TCCRAC	7 cut(s)	126, 882, 1261, 2446, 3198, 3280, 3351
Mox20I	TGGCCA	2 cut(s)	63, 3753
Mph1103I	ATGCAT	3 cut(s)	1180, 1693, 3131
MroI	TCCGGA	1 cut(s)	1622
MroXI	GAANNNNTTC	1 cut(s)	540
MscI	TGGCCA	2 cut(s)	63, 3753
MslI	CAYNNNNRTG	3 cut(s)	667, 2360, 3771
Msp20I	TGGCCA	2 cut(s)	63, 3753
MspA1I	CMGCKG	3 cut(s)	1151, 2012, 3214
MspCI	CTTAAG	1 cut(s)	3431
MspI	CCGG	7 cut(s)	37, 51, 70, 506, 1623, 3484, 4056
MspR9I	CCNGG	7 cut(s)	38, 507, 698, 1667, 2346, 3068, 3623
MunI	CAATTG	3 cut(s)	812, 2187, 3136
MvaI	CCWGG	5 cut(s)	698, 1667, 2346, 3068, 3623
MvnI	CGCG	4 cut(s)	20, 304, 2092, 2223
NarI	GGCGCC	1 cut(s)	343
NciI	CCSGG	2 cut(s)	38, 507
NcoI	CCATGG	1 cut(s)	3355
NdeI	CATATG	1 cut(s)	3131
NheI	GCTAGC	1 cut(s)	4040
NmuCI	GTSAC	6 cut(s)	1929, 1956, 2075, 3019, 4022, 4051
NsiI	ATGCAT	3 cut(s)	1180, 1693, 3131
NspI	RCATGY	3 cut(s)	2560, 2701, 3558
NspV	TTCGAA	1 cut(s)	3910
PaeI	GCATGC	1 cut(s)	3558
PaeR7I	CTCGAG	2 cut(s)	74, 3801
PagI	TCATGA	3 cut(s)	1033, 1759, 2511
PasI	CCCWGGG	1 cut(s)	1666
PceI	AGGCCT	1 cut(s)	656
PciI	ACATGT	1 cut(s)	2697
PcsI	WCGNNNNNNNCGW	1 cut(s)	99
PdmI	GAANNNNTTC	1 cut(s)	540
PfeI	GAWTC	8 cut(s)	164, 521, 827, 932, 1993, 2062, 2486, 3808
PflFI	GACNNNGTC	1 cut(s)	107
PfoI	TCCNGGA	2 cut(s)	505, 2344
PleI	GAGTC	5 cut(s)	436, 1115, 1762, 2840, 2902
PluTI	GGCGCC	1 cut(s)	346
PmaCI	CACGTG	1 cut(s)	324
PmlI	CACGTG	1 cut(s)	324
PpsI	GAGTC	5 cut(s)	436, 1115, 1762, 2840, 2902
Ppu21I	YACGTR	2 cut(s)	324, 1385
PscI	ACATGT	1 cut(s)	2697
PsiI	TTATAA	1 cut(s)	1466
Psp124BI	GAGCTC	2 cut(s)	101, 424
Psp6I	CCWGG	5 cut(s)	696, 1665, 2344, 3066, 3621
PspCI	CACGTG	1 cut(s)	324
PspFI	CCCAGC	1 cut(s)	3080
PspGI	CCWGG	5 cut(s)	696, 1665, 2344, 3066, 3621
PspPI	GGNCC	3 cut(s)	455, 637, 2818
PspXI	VCTCGAGB	1 cut(s)	74
PstNI	CAGNNNCTG	2 cut(s)	830, 2711
PsuI	RGATCY	6 cut(s)	4, 944, 2102, 3259, 3271, 3685
PsyI	GACNNNGTC	1 cut(s)	107
PvuII	CAGCTG	3 cut(s)	1151, 2012, 3214
RseI	CAYNNNNRTG	3 cut(s)	667, 2360, 3771
SacI	GAGCTC	2 cut(s)	101, 424
Sau96I	GGNCC	3 cut(s)	455, 637, 2818
SchI	GAGTC	5 cut(s)	437, 1115, 1762, 2841, 2902
ScrFI	CCNGG	7 cut(s)	38, 507, 698, 1667, 2346, 3068, 3623
SfaNI	GCATC	9 cut(s)	492, 1199, 1678, 1903, 2530, 2613, 3415, 3508, 3968
SfiI	GGCCNNNNNGGCC	1 cut(s)	60
SfoI	GGCGCC	1 cut(s)	344
Sfr274I	CTCGAG	2 cut(s)	74, 3801
SfuI	TTCGAA	1 cut(s)	3910
SinI	GGWCC	3 cut(s)	455, 637, 2818
SlaI	CTCGAG	2 cut(s)	74, 3801
SmiMI	CAYNNNNRTG	3 cut(s)	667, 2360, 3771
SmlI	CTYRAG	6 cut(s)	74, 423, 1306, 3431, 3801, 3812
SmoI	CTYRAG	6 cut(s)	74, 423, 1306, 3431, 3801, 3812
SphI	GCATGC	1 cut(s)	3558
SseBI	AGGCCT	1 cut(s)	656
SsiI	CCGC	8 cut(s)	18, 137, 302, 493, 923, 1800, 2223, 4038
SspDI	GGCGCC	1 cut(s)	342
SspI	AATATT	3 cut(s)	1347, 1489, 2733
SspMI	CTAG	6 cut(s)	150, 533, 2283, 2435, 2850, 4041
SstI	GAGCTC	2 cut(s)	101, 424
StuI	AGGCCT	1 cut(s)	656
StyD4I	CCNGG	7 cut(s)	36, 505, 696, 1665, 2344, 3066, 3621
StyI	CCWWGG	5 cut(s)	149, 285, 640, 3255, 3355
TaiI	ACGT	8 cut(s)	326, 448, 1387, 2306, 2992, 3291, 3938, 4053
TaqI	TCGA	8 cut(s)	75, 93, 258, 873, 1771, 3423, 3802, 3910
TatI	WGTACW	4 cut(s)	745, 2700, 2868, 3984
TauI	GCSGC	5 cut(s)	20, 139, 304, 926, 4040
TfiI	GAWTC	8 cut(s)	164, 521, 827, 932, 1993, 2062, 2486, 3808
TscAI	CASTG	9 cut(s)	64, 94, 254, 303, 1297, 1613, 2919, 3418, 3645
TseFI	GTSAC	6 cut(s)	1929, 1956, 2075, 3019, 4022, 4051
TseI	GCWGC	7 cut(s)	306, 1136, 1679, 2711, 3077, 3188, 3956
Tsp45I	GTSAC	6 cut(s)	1929, 1956, 2075, 3019, 4022, 4051
TspGWI	ACGGA	4 cut(s)	376, 1056, 3252, 3849
TspRI	CASTG	9 cut(s)	64, 94, 254, 303, 1297, 1613, 2919, 3418, 3645
Tth111I	GACNNNGTC	1 cut(s)	107
Vha464I	CTTAAG	1 cut(s)	3431
VneI	GTGCAC	1 cut(s)	1415
VpaK11BI	GGWCC	3 cut(s)	455, 637, 2818
XagI	CCTNNNNNAGG	1 cut(s)	2438
XbaI	TCTAGA	1 cut(s)	532
XceI	RCATGY	3 cut(s)	2560, 2701, 3558
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2156
XhoI	CTCGAG	2 cut(s)	74, 3801
XmaJI	CCTAGG	1 cut(s)	149
XmiI	GTMKAC	1 cut(s)	1217
XmnI	GAANNNNTTC	1 cut(s)	540
XspI	CTAG	6 cut(s)	150, 533, 2283, 2435, 2850, 4041
Zsp2I	ATGCAT	3 cut(s)	1180, 1693, 3131

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.