Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0005110

Physical Location & Seq

Genomic Coordinates Reverse (-)

73377119 .. 73389888

Gene Structure 12770 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G041740.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4074 bp

ATGGATCCGATACAAGCAGCCAAGGAGCTCTCATTCCGGGTCGGCTTCTCCGGCCACAGCGGCCACCTCCGCCTCGAGCCTCTCTGCACCGTCGAGCGCTCCGACCCCGTCAAGTCGCTCCCCGATTTCGTCCTGCCGCCTGCATTTGCTAGGGAAACGCCGGAGTCGATAAAAGAGTATATAGAGGAGACATATCTGTTGCCGAGATTGGATTCTGATGTCTTTTCACCGGAAAAGGTTGGGAGGCAGTGGGATTTTGACTGGTTTGACAAGGCTAATGTACACTTGGAGCCGTCGTTGCCACGGTCTGTTGTCGTTCCGACATGGGAGTTACCGTTTAGGCGCCAGAAGAATGGATCAGAAGGAGGGATATGGGAACCCAAATCTGTGCAGGTCGATGAAGCAGAACTTACCATAGAAGCACAAGAGTCTGGTTCGTTGCCACGCATGGCCGGACCAGCAAAGGATTTTGTATGGGGAAGCATCAGCAACCGTCCTTTTCGTCCAGGAGGCTTGGATGATTCCCAATCTCTAGAAAGAACTCTTCCTGATGGTGCTTCTAATGGCGAATGGGTACACCAACTTCTTACTGGTGGCCCTGCACAGGCTGTTCCACCAAGCTTTAAGCAAGGATTGGACCTTGGTCCTCTCAAGGCATATCCTGGCTCATGGAATGTATACAATGATCAAGGTTCAGTCAAGAGCACATCAGATGAAAAGCTGGGTTTGCAGAGTGAGTTGTCTGTACAGTTTGATGACTTGTTCAAAAAGGCTTGGGATGAGGATGTTGTTGAGTTTGAAGGAGATGGTCAATCGTCAGGATCGGAATCTGCTAAGTCAGAGGATGAAGCAAATGTGGTTGATGTTGATATTACCAGCAATCCTCCTGAGCCTGAACTGTCTGTGTTGGATGAGATTTTGTCAGTTGAGGCAGGAGACTCAAAGTCAAGATTTAATGGAACTGGTGGTGAACAGAACCCGGAGGCTTGGGCTATTAGTGGACGTACTGAATGGATTTCGGAGAATTTTCATGATCTTGTTCCTGATAAGGCACTTGATTTTCCCTTTGAATTGGATACATTCCAAAAGGAGGCAATTTATTATCTTGAGAAGGGGGAGTCTGTCTTTGTATCTGCTCACACATCAGCTGGAAAGACAGTAGTTGCAGAATATGCATTTGCTTTGGCATCAAAACATTGCACGAGAGCTGTGTATACTGCTCCTATCAAGACCATCAGCAACCAAAAATATAGGGACTTCTGTGGAAAATTCGATGTTGGACTTCTCACTGGTGATGTTAGCTTGAGGCCCGAGGCTTCTTGTCTCATTATGACCACGGAAATATTAAGGTCAATGCTTTATCGGGGTGCTGACATCATACGTGACATTGAATGGGTTATCTTTGATGAGGTTCATTATGTCAATGATGTCGAAAGAGGTGTTGTTTGGGAAGAAGTTATTATAATGCTCCCGAGACACATCAACATTGTCCTCCTTTCAGCTACGGTACCGAACAAAGTTGAGTTTGCTGACTGGATTGGACGGACAAAGCAAAAGCAAATCCGTGTTACTGGGACTACAAAAAGACCAGTCCCATTGGAGCACTGCCTATTTTACTCCGGAGAACTTTACAAGATATGTGAAAGTGAAAGCTTCATTCCTCAGGGCTTCAAAGCTGCAAAAGATACATTCAAGAAAAAGAATAGTCCTGCCACCGGCGGCGGCGGCGGTGGATCACGTGCTCCAGCTTCTGCTTCAGCTTCTCACGATGGGGCTCGAGGTCAAAAACGTGAAACTTCACATTCAGGAAAACAGAAGCAATTGGGTGCCCATAACTCAGGGAATTTATCCAGAACTGGTGGAGCAAATCAAAACAACGGCAATGGCATGAACAGTTGGGGTTTAAGAAGATCAGATGCTTCTTCGTGGTTGTCACTTATTAACAAACTCTCAAAGAAGTCACTATTACCTGTGGTTATATTTTGTTTCTCAAAGAATCGCTGTGATAGGTCAGCTGATAGCATGTCGGGAATTGACCTCACAAGTAGTTCTGAGAAAAGTCAGATTCGTGTTTTCTGTGACAAAGCATTTTCAAGGCTGAAGGGATCCGACAGGAATTTACCTCAGGTTGTCAGAGTTCAAAACCTTCTCCATAGAGGGATTGGTGTCCATCATGCAGGACTTCTTCCAATTGTTAAGGAAGTTGTTGAAATGCTCTTTTGTCGAGGTGTGATAAAGGTTCTGTTTTCAACAGAGACATTTGCAATGGGAGTGAATGCACCAGCTAGAACGGTTGTCTTTGATACATTAAGGAAATTTGATGGCAAGGAATTTAGACAACTGCTACCAGGGGAATACACTCAAATGGCAGGCCGTGCTGGCAGGAGAGGACTTGATAAAATTGGTACAGTTATTGTAATGTGCCGTGATGAAATCCTAGGAGAGGGGGATTTGAAGCATGTCATAGTTGGAAGTGCAACCAGGCTAGAATCTCAGTTTCGGCTTACTTATATTATGATCATGCATCTTCTTCGTGTTGAAGAACTGAAGGTGGAGGACATGCTGAAAAGAAGTTTTGCTGAGTTCCATGCTCAGAAGAAACTACCAGATATGCAGCAGCTTCTGATGCGAAAGCTTGCTCAGCCTACAAAAAGTATCGAATGTATAAAAGGGGAACCAGCTATTGAGGAGTATTATGACATGCACTTAGAAAAGGAGAAGCATAGAACAGAAGTATTGGAGGCAGTAATGCAGTCCCCTGGTGCCCAACAATTTCTTACATCCGGAAGAGTGGTTGTCATGAAATCACAATCAGCCCAGAAATACTTGCTTGGAGTTGTTGTAAAAGCACCTTCTTCTAGTAATAAGCAACACATCGTTCTAGTGCTGAAACCTGAACTACCAGCGACAATCCAAACTCCCCTGGCTAGTGGTAACTTACAAGATACCAAAAACACTGATTCTTCTCAGGGTTACTACGTGGTAGCGAAATCTAAACGTGCTTTTGAAGAAGAGTATTGTACCTCTGTTTCTTCACGCAAAGGATCAGGTGTCATCAACATAAAATTACCACACCATGGTGCTGCTGCTGGGGTGAGATATGAGGTTAGAGGAGCCGATATCACAGACTTCTCATTCATATGCAGTAGCAAAATAAAGATTGACCTAGTCCGGCTTCTTGAGGACTGTAGCAGTGCTGCTTACTCCAAGACAGTGCAACTGCTGTTGGACACAAAACCTAAAGGAGAGGAGTACCCTCTAGCCTTGGATCCAGTGAAAGATCTGAAGTTGAAAGACATGCATCTTGTGGAAACATACTACAAATGGACAAACTTAGTGCAGAAGATGGCAAAGAATAAGTGCGATGGATGTATTAAATTGGAGGAACACATTCATTTAGCAAGAGAGATAAAGAGACACAAAGAGGAAGTTAATGCTCTTAAGTTTCAAATGTCAGATGAAGCACTTCAACAGATGCCAGATTTTCAAGGCCGGATAGATGTTCTGAAGGAAATCGGATGTATAGATGCTGACCTTGTAGTTCAAATAAAAGGCCGTGTCGCATGCGAGATGAATTCTGGGGAGGAGTTGATCTGCACAGAGTGTTTGTTTGAGAACCAACTAGATGACCTGGAACCAGAAGAAGCGGTGGCATTAATGTCGTCCTTCGTTTTTCAGCAGAAGAATACTTCTGAACCTTCACTTACTCCTAAACTTTCTATGGCGAAAGAGAGATTGTACAATACAGCAATAAGACTAGGCAAGTGTCAAGCTTACTTCAAACTTCCAATAAATCCAGAAGAGTACGCCAAAGAGAATCTCAAGTTTGGTCTTGTTGAAGTCGTTTATGAGTGGGCAAAGGGCACACCATTTGCAGATATTTGTGAACTCACAGATGTTCCTGAAGGCATGATAGTCCGGACCATTGTTAGACTTGATGAGACGTGTCGTGAATTCAAAAATGCTGCATCTATTATGGGTAATTCTGCTCTATACAAGAAAATGGAGACAGCTTCAAATGCAATAAAACGTGACATTGTTTTCGCAGCTAGCTTGTACGTAACAGGAGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000184 GO:0000288 GO:0000291 GO:0000956 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010035 GO:0010038 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0015672 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0019439 GO:0030001 GO:0032991 GO:0034427 GO:0034641 GO:0034655 GO:0035864 GO:0040029 GO:0042221 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0050789 GO:0050896 GO:0051179 GO:0051234 GO:0055087 GO:0060255 GO:0065007 GO:0070035 GO:0070478 GO:0071704 GO:0090304 GO:0140098 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

DEAD DSHCT Helicase_C rRNA_proc-arch

Protein Analysis

1357

Amino Acids

151.07

Weight (kDa)

5.98

Isoelectric Point (pI)

43.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ski2_N	PF17911	114 - 215	4.7e-09	Ski2 N-terminal region
DEAD	PF00270	361 - 508	5.2e-23	DEAD/DEAH box helicase
Helicase_C	PF00271	684 - 797	6e-06	Helicase conserved C-terminal domain
MTR4-like_stalk	PF21408	852 - 916	9.6e-13	Exosome RNA helicase MTR4-like, stalk
MTR4_beta-barrel	PF13234	920 - 1153	4.9e-24	Mtr4-like, beta-barrel domain
DSHCT	PF08148	1179 - 1349	1.7e-58	DSHCT (NUC185) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0005110)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46960 AT3G46960 AT3G46960
fragaria_vesca	FvH4_3g37880 FvH4_3g37880 FvH4_3g37880 FvH4_3g37880 FvH4_3g37880
malus_domestica	MD03G1077900.v1.1 MD11G1082500.v1.1
prunus_persica	Prupe.6G062900_v2.0.a1 Prupe.6G062900_v2.0.a1 Prupe.6G062900_v2.0.a1
pyrus_communis	pycom03g06210 pycom11g07060
rosa_chinensis	RchiOBHm_Chr5g0068091
rosa_laevigata	RLG00000035954
rosa_multiflora	Rmu_sc0000435.1_g000017
rosa_roxburghii	Rroxscaffold_1G00012520 Rroxscaffold_1G00013260
rosa_rugosa	Rorug05G0387900 Rorug05G0388300 Rorug05G0388400 Rorug05G0388500
rosa_samantha	Rh5AG446500 Rh5BG464600 Rh5CG486400 Rh5DG478400
rosa_wichuraiana	Rw5G041700 Rw5G041740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1463
Acc36I	ACCTGC	1 cut(s)	382
Acc65I	GGTACC	1 cut(s)	1507
AccB1I	GGYRCC	4 cut(s)	342, 1507, 1826, 2762
AccB7I	CCANNNNNTGG	1 cut(s)	3077
AccI	GTMKAC	2 cut(s)	678, 1214
AccIII	TCCGGA	3 cut(s)	1619, 2783, 3918
AciI	CCGC	8 cut(s)	60, 70, 137, 1719, 1722, 1725, 1728, 3647
AclWI	GGATC	9 cut(s)	12, 364, 829, 1741, 2100, 2113, 3052, 3263, 3276
AcoI	YGGCCR	3 cut(s)	52, 61, 450
AcsI	RAATTY	8 cut(s)	1024, 1268, 1843, 2116, 2315, 2330, 3574, 3953
AcuI	CTGAAG	6 cut(s)	1740, 2120, 2567, 3305, 3527, 3924
AcvI	CACGTG	1 cut(s)	1739
AcyI	GRCGYC	1 cut(s)	343
AdeI	CACNNNGTG	1 cut(s)	3603
AfeI	AGCGCT	1 cut(s)	98
AfiI	CCNNNNNNNGG	5 cut(s)	604, 1090, 1715, 2443, 3077
AflII	CTTAAG	1 cut(s)	3440
AflIII	ACRYGT	1 cut(s)	3944
AhdI	GACNNNNNGTC	1 cut(s)	943
AjiI	CACGTC	1 cut(s)	3945
AjnI	CCWGG	7 cut(s)	505, 661, 2347, 2480, 2758, 2922, 3630
AleI	CACNNNNGTG	1 cut(s)	3078
Alw21I	GWGCWC	4 cut(s)	30, 707, 1605, 1744
Alw26I	GTCTC	8 cut(s)	182, 930, 1328, 1468, 2249, 3409, 3935, 4001
AlwI	GGATC	9 cut(s)	12, 364, 829, 1741, 2100, 2113, 3052, 3263, 3276
AlwNI	CAGNNNCTG	2 cut(s)	1751, 2623
Ama87I	CYCGRG	4 cut(s)	74, 1310, 1471, 1776
Aor13HI	TCCGGA	3 cut(s)	1619, 2783, 3918
Aor51HI	AGCGCT	1 cut(s)	98
AoxI	GGCC	8 cut(s)	52, 61, 450, 595, 1307, 2371, 3490, 3553
ApeKI	GCWGC	9 cut(s)	17, 1676, 2614, 2617, 3083, 3086, 3197, 3965, 4046
ApoI	RAATTY	8 cut(s)	1024, 1268, 1843, 2116, 2315, 2330, 3574, 3953
ArsI	GACNNNNNNTTYG	2 cut(s)	4025, 4057
AseI	ATTAAT	1 cut(s)	3656
Asp700I	GAANNNNTTC	2 cut(s)	3390, 3465
Asp718I	GGTACC	1 cut(s)	1507
AspA2I	CCTAGG	1 cut(s)	2437
AspLEI	GCGC	2 cut(s)	99, 345
AspS9I	GGNCC	6 cut(s)	455, 596, 637, 644, 1308, 3921
AsuC2I	CCSGG	2 cut(s)	38, 980
AsuHPI	GGTGA	4 cut(s)	219, 980, 1304, 3106
AsuNHI	GCTAGC	1 cut(s)	4049
AvaI	CYCGRG	4 cut(s)	74, 1310, 1471, 1776
AvaII	GGWCC	4 cut(s)	455, 637, 644, 3921
AvrII	CCTAGG	1 cut(s)	2437
AxyI	CCTNAGG	2 cut(s)	1662, 2124
BaeGI	GKGCMC	3 cut(s)	1831, 2767, 3866
BamHI	GGATCC	3 cut(s)	4, 2105, 3268
BanI	GGYRCC	4 cut(s)	342, 1507, 1826, 2762
BanII	GRGCYC	2 cut(s)	30, 1777
BarI	GAAGNNNNNNTAC	2 cut(s)	567, 599
BauI	CACGAG	1 cut(s)	1201
BbrPI	CACGTG	1 cut(s)	1739
Bbv12I	GWGCWC	4 cut(s)	30, 707, 1605, 1744
BbvI	GCAGC	9 cut(s)	29, 1663, 2626, 2629, 3070, 3073, 3184, 3952, 4058
BccI	CCATC	8 cut(s)	545, 800, 1241, 1763, 2178, 2315, 3340, 3359
BceAI	ACGGC	5 cut(s)	277, 1894, 2358, 2409, 3540
BcgI	CGANNNNNNTGC	4 cut(s)	2513, 2547, 3642, 3676
BciT130I	CCWGG	7 cut(s)	507, 663, 2349, 2482, 2760, 2924, 3632
BciVI	GTATCC	1 cut(s)	1069
BclI	TGATCA	2 cut(s)	685, 2517
BcnI	CCSGG	2 cut(s)	38, 980
BcoDI	GTCTC	8 cut(s)	182, 930, 1328, 1468, 2249, 3409, 3935, 4001
BfmI	CTRYAG	1 cut(s)	3187
BfoI	RGCGCY	2 cut(s)	100, 346
BfrI	CTTAAG	1 cut(s)	3440
BfuAI	ACCTGC	1 cut(s)	382
BfuI	GTATCC	1 cut(s)	1069
BglI	GCCNNNNNGGC	2 cut(s)	60, 2379
BglII	AGATCT	1 cut(s)	3280
BlnI	CCTAGG	1 cut(s)	2437
BlpI	GCTNAGC	1 cut(s)	2640
Bme1390I	CCNGG	9 cut(s)	38, 507, 663, 980, 2349, 2482, 2760, 2924, 3632
Bme18I	GGWCC	4 cut(s)	455, 637, 644, 3921
BmeRI	GACNNNNNGTC	1 cut(s)	943
BmeT110I	CYCGRG	4 cut(s)	74, 1310, 1471, 1776
BmgBI	CACGTC	1 cut(s)	3945
BmgT120I	GGNCC	6 cut(s)	455, 596, 637, 644, 1308, 3921
BmrFI	CCNGG	9 cut(s)	38, 507, 663, 980, 2349, 2482, 2760, 2924, 3632
BmrI	ACTGGG	1 cut(s)	1581
BmsI	GCATC	9 cut(s)	492, 1196, 1906, 2533, 2616, 3310, 3465, 3517, 3977
BmtI	GCTAGC	1 cut(s)	4053
BmuI	ACTGGG	1 cut(s)	1581
BoxI	GACNNNNGTC	1 cut(s)	642
BpmI	CTGGAG	1 cut(s)	1728
Bpu10I	CCTNAGC	1 cut(s)	888
Bpu1102I	GCTNAGC	1 cut(s)	2640
BpuEI	CTTGAG	5 cut(s)	635, 1127, 1324, 3200, 3806
BpuMI	CCSGG	2 cut(s)	38, 980
BsaAI	YACGTR	4 cut(s)	1382, 1739, 2980, 4060
BsaBI	GATNNNNATC	1 cut(s)	826
BsaHI	GRCGYC	1 cut(s)	343
BsaWI	WCCGGW	4 cut(s)	229, 1619, 2783, 3918
BsaXI	ACNNNNNCTCC	6 cut(s)	281, 311, 320, 350, 2826, 2856
Bsc4I	CCNNNNNNNGG	5 cut(s)	604, 1090, 1715, 2443, 3077
Bse118I	RCCGGY	1 cut(s)	1715
Bse1I	ACTGG	9 cut(s)	266, 595, 967, 1294, 1538, 1576, 1589, 1861, 3272
Bse21I	CCTNAGG	2 cut(s)	1662, 2124
Bse3DI	GCAATG	3 cut(s)	1195, 1888, 2271
Bse8I	GATNNNNATC	1 cut(s)	826
BseAI	TCCGGA	3 cut(s)	1619, 2783, 3918
BseBI	CCWGG	7 cut(s)	507, 663, 2349, 2482, 2760, 2924, 3632
BseGI	GGATG	8 cut(s)	523, 784, 790, 850, 916, 2780, 3374, 3524
BseJI	GATNNNNATC	1 cut(s)	826
BseLI	CCNNNNNNNGG	5 cut(s)	604, 1090, 1715, 2443, 3077
BseMI	GCAATG	3 cut(s)	1195, 1888, 2271
BseNI	ACTGG	9 cut(s)	266, 595, 967, 1294, 1538, 1576, 1589, 1861, 3272
BseRI	GAGGAG	5 cut(s)	200, 2702, 3126, 3263, 3599
BseSI	GKGCMC	3 cut(s)	1831, 2767, 3866
BseXI	GCAGC	9 cut(s)	29, 1663, 2626, 2629, 3070, 3073, 3184, 3952, 4058
BseYI	CCCAGC	2 cut(s)	721, 3089
BsgI	GTGCAG	5 cut(s)	70, 410, 585, 3359, 3580
BshFI	GGCC	8 cut(s)	54, 63, 452, 597, 1309, 2373, 3492, 3555
BshNI	GGYRCC	4 cut(s)	342, 1507, 1826, 2762
BsiHKAI	GWGCWC	4 cut(s)	30, 707, 1605, 1744
BsiHKCI	CYCGRG	4 cut(s)	74, 1310, 1471, 1776
BslFI	GGGAC	4 cut(s)	1268, 1577, 1588, 2740
BslI	CCNNNNNNNGG	5 cut(s)	604, 1090, 1715, 2443, 3077
BsmAI	GTCTC	8 cut(s)	182, 930, 1328, 1468, 2249, 3409, 3935, 4001
BsmBI	CGTCTC	1 cut(s)	3935
BsmFI	GGGAC	4 cut(s)	1268, 1577, 1588, 2740
BsmI	GAATGC	1 cut(s)	2281
BsnI	GGCC	8 cut(s)	54, 63, 452, 597, 1309, 2373, 3492, 3555
BsoBI	CYCGRG	4 cut(s)	74, 1310, 1471, 1776
Bsp1286I	GDGCHC	8 cut(s)	30, 707, 1605, 1744, 1777, 1831, 2767, 3866
Bsp13I	TCCGGA	3 cut(s)	1619, 2783, 3918
Bsp1407I	TGTACA	3 cut(s)	280, 745, 3738
Bsp1720I	GCTNAGC	1 cut(s)	2640
Bsp19I	CCATGG	1 cut(s)	3076
BspACI	CCGC	8 cut(s)	60, 70, 137, 1719, 1722, 1725, 1728, 3647
BspANI	GGCC	8 cut(s)	54, 63, 452, 597, 1309, 2373, 3492, 3555
BspEI	TCCGGA	3 cut(s)	1619, 2783, 3918
BspHI	TCATGA	2 cut(s)	1030, 2799
BspMI	ACCTGC	1 cut(s)	382
BspOI	GCTAGC	1 cut(s)	4053
BspPI	GGATC	9 cut(s)	12, 364, 829, 1741, 2100, 2113, 3052, 3263, 3276
BspT107I	GGYRCC	4 cut(s)	342, 1507, 1826, 2762
BspTI	CTTAAG	1 cut(s)	3440
BsrDI	GCAATG	3 cut(s)	1195, 1888, 2271
BsrFI	RCCGGY	1 cut(s)	1715
BsrGI	TGTACA	3 cut(s)	280, 745, 3738
BsrI	ACTGG	9 cut(s)	266, 595, 967, 1294, 1538, 1576, 1589, 1861, 3272
BssAI	RCCGGY	1 cut(s)	1715
BssNAI	GTATAC	2 cut(s)	679, 1215
BssNI	GRCGYC	1 cut(s)	343
BssSI	CACGAG	1 cut(s)	1201
BssT1I	CCWWGG	5 cut(s)	21, 640, 2437, 3076, 3264
Bst1107I	GTATAC	2 cut(s)	679, 1215
Bst2BI	CACGAG	1 cut(s)	1201
Bst2UI	CCWGG	7 cut(s)	507, 663, 2349, 2482, 2760, 2924, 3632
Bst6I	CTCTTC	4 cut(s)	549, 2782, 3006, 3795
BstACI	GRCGYC	1 cut(s)	343
BstAFI	CTTAAG	1 cut(s)	3440
BstAPI	GCANNNNNTGC	2 cut(s)	1172, 2375
BstAUI	TGTACA	3 cut(s)	280, 745, 3738
BstBAI	YACGTR	4 cut(s)	1382, 1739, 2980, 4060
BstC8I	GCNNGC	6 cut(s)	141, 2371, 2380, 2637, 3565, 4051
BstDSI	CCRYGG	3 cut(s)	302, 1335, 3076
BstENI	CCTNNNNNAGG	1 cut(s)	2441
BstF5I	GGATG	8 cut(s)	523, 784, 790, 850, 916, 2780, 3374, 3524
BstH2I	RGCGCY	2 cut(s)	100, 346
BstHHI	GCGC	2 cut(s)	99, 345
BstMAI	GTCTC	8 cut(s)	182, 930, 1328, 1468, 2249, 3409, 3935, 4001
BstNI	CCWGG	7 cut(s)	507, 663, 2349, 2482, 2760, 2924, 3632
BstNSI	RCATGY	6 cut(s)	2026, 2462, 2563, 2704, 3301, 3567
BstPAI	GACNNNNGTC	1 cut(s)	642
BstSCI	CCNGG	9 cut(s)	36, 505, 661, 978, 2347, 2480, 2758, 2922, 3630
BstSFI	CTRYAG	1 cut(s)	3187
BstSLI	GKGCMC	3 cut(s)	1831, 2767, 3866
BstSNI	TACGTA	1 cut(s)	4060
BstV1I	GCAGC	9 cut(s)	29, 1663, 2626, 2629, 3070, 3073, 3184, 3952, 4058
BstX2I	RGATCY	4 cut(s)	4, 2105, 3268, 3280
BstXI	CCANNNNNNTGG	1 cut(s)	353
BstYI	RGATCY	4 cut(s)	4, 2105, 3268, 3280
BstZ17I	GTATAC	2 cut(s)	679, 1215
Bsu36I	CCTNAGG	2 cut(s)	1662, 2124
BsuI	GTATCC	1 cut(s)	1069
BsuRI	GGCC	8 cut(s)	54, 63, 452, 597, 1309, 2373, 3492, 3555
BtgI	CCRYGG	3 cut(s)	302, 1335, 3076
BtgZI	GCGATG	1 cut(s)	3378
BtrI	CACGTC	1 cut(s)	3945
BtsCI	GGATG	8 cut(s)	523, 784, 790, 850, 916, 2780, 3374, 3524
BtsI	GCAGTG	3 cut(s)	254, 1603, 3199
BtsIMutI	CAGTG	7 cut(s)	254, 1287, 1603, 2955, 3199, 3219, 3279
BveI	ACCTGC	1 cut(s)	382
Cac8I	GCNNGC	6 cut(s)	141, 2371, 2380, 2637, 3565, 4051
CaiI	CAGNNNCTG	2 cut(s)	1751, 2623
CciI	TCATGA	2 cut(s)	1030, 2799
CfoI	GCGC	2 cut(s)	99, 345
Cfr10I	RCCGGY	1 cut(s)	1715
Cfr13I	GGNCC	6 cut(s)	455, 596, 637, 644, 1308, 3921
DinI	GGCGCC	1 cut(s)	344
DraIII	CACNNNGTG	1 cut(s)	3603
DriI	GACNNNNNGTC	1 cut(s)	943
EaeI	YGGCCR	3 cut(s)	52, 61, 450
Eam1104I	CTCTTC	4 cut(s)	549, 2782, 3006, 3795
Eam1105I	GACNNNNNGTC	1 cut(s)	943
EarI	CTCTTC	4 cut(s)	549, 2782, 3006, 3795
EciI	GGCGGA	1 cut(s)	59
Ecl136II	GAGCTC	1 cut(s)	28
Eco105I	TACGTA	1 cut(s)	4060
Eco130I	CCWWGG	5 cut(s)	21, 640, 2437, 3076, 3264
Eco24I	GRGCYC	2 cut(s)	30, 1777
Eco32I	GATATC	1 cut(s)	3121
Eco47I	GGWCC	4 cut(s)	455, 637, 644, 3921
Eco47III	AGCGCT	1 cut(s)	98
Eco53kI	GAGCTC	1 cut(s)	28
Eco57I	CTGAAG	6 cut(s)	1740, 2120, 2567, 3305, 3527, 3924
Eco72I	CACGTG	1 cut(s)	1739
Eco81I	CCTNAGG	2 cut(s)	1662, 2124
Eco88I	CYCGRG	4 cut(s)	74, 1310, 1471, 1776
EcoICRI	GAGCTC	1 cut(s)	28
EcoNI	CCTNNNNNAGG	1 cut(s)	2441
EcoRI	GAATTC	2 cut(s)	3574, 3953
EcoRII	CCWGG	7 cut(s)	505, 661, 2347, 2480, 2758, 2922, 3630
EcoRV	GATATC	1 cut(s)	3121
EcoT14I	CCWWGG	5 cut(s)	21, 640, 2437, 3076, 3264
EcoT22I	ATGCAT	3 cut(s)	1177, 2526, 3303
EcoT38I	GRGCYC	2 cut(s)	30, 1777
EgeI	GGCGCC	1 cut(s)	344
EheI	GGCGCC	1 cut(s)	344
ErhI	CCWWGG	5 cut(s)	21, 640, 2437, 3076, 3264
Esp3I	CGTCTC	1 cut(s)	3935
FalI	AAGNNNNNCTT	2 cut(s)	393, 425
FaqI	GGGAC	4 cut(s)	1268, 1577, 1588, 2740
FauNDI	CATATG	1 cut(s)	3140
FbaI	TGATCA	2 cut(s)	685, 2517
FblI	GTMKAC	2 cut(s)	678, 1214
FokI	GGATG	8 cut(s)	530, 791, 797, 857, 923, 2767, 3381, 3531
FriOI	GRGCYC	2 cut(s)	30, 1777
GlaI	GCGC	2 cut(s)	98, 344
GsaI	CCCAGC	2 cut(s)	725, 3093
GsuI	CTGGAG	1 cut(s)	1728
HaeII	RGCGCY	2 cut(s)	100, 346
HaeIII	GGCC	8 cut(s)	54, 63, 452, 597, 1309, 2373, 3492, 3555
HhaI	GCGC	2 cut(s)	99, 345
Hin1I	GRCGYC	1 cut(s)	343
Hin6I	GCGC	2 cut(s)	97, 343
HinP1I	GCGC	2 cut(s)	97, 343
HindIII	AAGCTT	4 cut(s)	619, 1651, 2633, 3771
HphI	GGTGA	4 cut(s)	219, 980, 1304, 3106
Hpy166II	GTNNAC	7 cut(s)	283, 577, 679, 971, 1001, 1215, 3887
Hpy8I	GTNNAC	7 cut(s)	283, 577, 679, 971, 1001, 1215, 3887
Hpy99I	CGWCG	2 cut(s)	95, 298
HpyCH4IV	ACGT	9 cut(s)	1003, 1381, 1738, 1789, 2979, 2998, 3944, 4030, 4059
HpySE526I	ACGT	9 cut(s)	1003, 1381, 1738, 1789, 2979, 2998, 3944, 4030, 4059
Hsp92I	GRCGYC	1 cut(s)	343
HspAI	GCGC	2 cut(s)	97, 343
KasI	GGCGCC	1 cut(s)	342
Kpn2I	TCCGGA	3 cut(s)	1619, 2783, 3918
KpnI	GGTACC	1 cut(s)	1511
Ksp22I	TGATCA	2 cut(s)	685, 2517
Lsp1109I	GCAGC	9 cut(s)	29, 1663, 2626, 2629, 3070, 3073, 3184, 3952, 4058
LweI	GCATC	9 cut(s)	492, 1196, 1906, 2533, 2616, 3310, 3465, 3517, 3977
MaeII	ACGT	9 cut(s)	1003, 1381, 1738, 1789, 2979, 2998, 3944, 4030, 4059
MfeI	CAATTG	2 cut(s)	1820, 2190
MflI	RGATCY	4 cut(s)	4, 2105, 3268, 3280
MhlI	GDGCHC	8 cut(s)	30, 707, 1605, 1744, 1777, 1831, 2767, 3866
Mly113I	GGCGCC	1 cut(s)	343
MlyI	GAGTC	4 cut(s)	173, 437, 932, 1127
MmeI	TCCRAC	7 cut(s)	126, 344, 888, 1258, 2133, 2449, 3207
Mph1103I	ATGCAT	3 cut(s)	1177, 2526, 3303
MroI	TCCGGA	3 cut(s)	1619, 2783, 3918
MroXI	GAANNNNTTC	2 cut(s)	3390, 3465
MslI	CAYNNNNRTG	3 cut(s)	2363, 3078, 3139
MspA1I	CMGCKG	3 cut(s)	60, 1148, 2015
MspCI	CTTAAG	1 cut(s)	3440
MspR9I	CCNGG	9 cut(s)	38, 507, 663, 980, 2349, 2482, 2760, 2924, 3632
MunI	CAATTG	2 cut(s)	1820, 2190
Mva1269I	GAATGC	1 cut(s)	2281
MvaI	CCWGG	7 cut(s)	507, 663, 2349, 2482, 2760, 2924, 3632
NarI	GGCGCC	1 cut(s)	343
NciI	CCSGG	2 cut(s)	38, 980
NcoI	CCATGG	1 cut(s)	3076
NdeI	CATATG	1 cut(s)	3140
NheI	GCTAGC	1 cut(s)	4049
NmeAIII	GCCGAG	1 cut(s)	228
NmuCI	GTSAC	5 cut(s)	1382, 1932, 1959, 2078, 4031
NsiI	ATGCAT	3 cut(s)	1177, 2526, 3303
NspI	RCATGY	6 cut(s)	2026, 2462, 2563, 2704, 3301, 3567
OliI	CACNNNNGTG	1 cut(s)	3078
PaeI	GCATGC	1 cut(s)	3567
PaeR7I	CTCGAG	2 cut(s)	74, 1776
PagI	TCATGA	2 cut(s)	1030, 2799
PcsI	WCGNNNNNNNCGW	2 cut(s)	99, 164
PctI	GAATGC	1 cut(s)	2281
PdmI	GAANNNNTTC	2 cut(s)	3390, 3465
PfeI	GAWTC	8 cut(s)	212, 521, 827, 1996, 2065, 2489, 2960, 3817
PflFI	GACNNNGTC	2 cut(s)	107, 3167
PflMI	CCANNNNNTGG	1 cut(s)	3077
PfoI	TCCNGGA	1 cut(s)	505
PleI	GAGTC	4 cut(s)	172, 436, 932, 1126
PluTI	GGCGCC	1 cut(s)	346
PmaCI	CACGTG	1 cut(s)	1739
PmlI	CACGTG	1 cut(s)	1739
PpsI	GAGTC	4 cut(s)	172, 436, 932, 1126
Ppu21I	YACGTR	4 cut(s)	1382, 1739, 2980, 4060
PshAI	GACNNNNGTC	1 cut(s)	642
PshBI	ATTAAT	1 cut(s)	3656
PsiI	TTATAA	1 cut(s)	1463
Psp124BI	GAGCTC	1 cut(s)	30
Psp6I	CCWGG	7 cut(s)	505, 661, 2347, 2480, 2758, 2922, 3630
PspCI	CACGTG	1 cut(s)	1739
PspFI	CCCAGC	2 cut(s)	721, 3089
PspGI	CCWGG	7 cut(s)	505, 661, 2347, 2480, 2758, 2922, 3630
PspPI	GGNCC	6 cut(s)	455, 596, 637, 644, 1308, 3921
PspXI	VCTCGAGB	2 cut(s)	74, 1776
PstNI	CAGNNNCTG	2 cut(s)	1751, 2623
PsuI	RGATCY	4 cut(s)	4, 2105, 3268, 3280
PsyI	GACNNNGTC	2 cut(s)	107, 3167
PvuII	CAGCTG	2 cut(s)	1148, 2015
RseI	CAYNNNNRTG	3 cut(s)	2363, 3078, 3139
SacI	GAGCTC	1 cut(s)	30
Sau96I	GGNCC	6 cut(s)	455, 596, 637, 644, 1308, 3921
SchI	GAGTC	4 cut(s)	173, 437, 932, 1127
ScrFI	CCNGG	9 cut(s)	38, 507, 663, 980, 2349, 2482, 2760, 2924, 3632
SduI	GDGCHC	8 cut(s)	30, 707, 1605, 1744, 1777, 1831, 2767, 3866
SfaNI	GCATC	9 cut(s)	492, 1196, 1906, 2533, 2616, 3310, 3465, 3517, 3977
SfcI	CTRYAG	1 cut(s)	3187
SfiI	GGCCNNNNNGGCC	1 cut(s)	60
SfoI	GGCGCC	1 cut(s)	344
Sfr274I	CTCGAG	2 cut(s)	74, 1776
SgrAI	CRCCGGYG	1 cut(s)	1715
SinI	GGWCC	4 cut(s)	455, 637, 644, 3921
SlaI	CTCGAG	2 cut(s)	74, 1776
SmiMI	CAYNNNNRTG	3 cut(s)	2363, 3078, 3139
SmlI	CTYRAG	8 cut(s)	74, 650, 1106, 1303, 1776, 3179, 3440, 3821
SmoI	CTYRAG	8 cut(s)	74, 650, 1106, 1303, 1776, 3179, 3440, 3821
SnaBI	TACGTA	1 cut(s)	4060
SphI	GCATGC	1 cut(s)	3567
SsiI	CCGC	8 cut(s)	60, 70, 137, 1719, 1722, 1725, 1728, 3647
SspDI	GGCGCC	1 cut(s)	342
SspI	AATATT	1 cut(s)	1344
SstI	GAGCTC	1 cut(s)	30
StyD4I	CCNGG	9 cut(s)	36, 505, 661, 978, 2347, 2480, 2758, 2922, 3630
StyI	CCWWGG	5 cut(s)	21, 640, 2437, 3076, 3264
TaiI	ACGT	9 cut(s)	1006, 1384, 1741, 1792, 2982, 3001, 3947, 4033, 4062
TaqI	TCGA	9 cut(s)	75, 93, 167, 396, 1272, 1431, 1777, 2224, 2658
TatI	WGTACW	3 cut(s)	280, 745, 3738
TauI	GCSGC	5 cut(s)	63, 139, 1722, 1725, 1728
TfiI	GAWTC	8 cut(s)	212, 521, 827, 1996, 2065, 2489, 2960, 3817
TscAI	CASTG	7 cut(s)	254, 1294, 1610, 2962, 3199, 3219, 3279
TseFI	GTSAC	5 cut(s)	1382, 1932, 1959, 2078, 4031
TseI	GCWGC	9 cut(s)	17, 1676, 2614, 2617, 3083, 3086, 3197, 3965, 4046
Tsp45I	GTSAC	5 cut(s)	1382, 1932, 1959, 2078, 4031
TspGWI	ACGGA	3 cut(s)	1352, 1553, 1558
TspRI	CASTG	7 cut(s)	254, 1294, 1610, 2962, 3199, 3219, 3279
Tth111I	GACNNNGTC	2 cut(s)	107, 3167
Van91I	CCANNNNNTGG	1 cut(s)	3077
Vha464I	CTTAAG	1 cut(s)	3440
VpaK11BI	GGWCC	4 cut(s)	455, 637, 644, 3921
VspI	ATTAAT	1 cut(s)	3656
XagI	CCTNNNNNAGG	1 cut(s)	2441
XapI	RAATTY	8 cut(s)	1024, 1268, 1843, 2116, 2315, 2330, 3574, 3953
XbaI	TCTAGA	1 cut(s)	532
XceI	RCATGY	6 cut(s)	2026, 2462, 2563, 2704, 3301, 3567
XcmI	CCANNNNNNNNNTGG	2 cut(s)	587, 2159
XhoI	CTCGAG	2 cut(s)	74, 1776
XmaJI	CCTAGG	1 cut(s)	2437
XmiI	GTMKAC	2 cut(s)	678, 1214
XmnI	GAANNNNTTC	2 cut(s)	3390, 3465
Zsp2I	ATGCAT	3 cut(s)	1177, 2526, 3303

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.