Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0005110

Physical Location & Seq

Genomic Coordinates Forward (+)

16301817 .. 16314840

Gene Structure 13024 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00013260.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3309 bp

ATGGATCCGATACAAGCAGCCAAGGAGCTCTCATTCCGGGTCGGCTTCTCCGGCCACAGCGGCCACCTCCGCCTCGAGCCTCTCTGCACCGTCGAGCGCTCCTACCCCGTCAAGTCGCTCCCCGATTTCGTCCTGCCGCCTGCATTTGCTAGGGAAACGCCGGAGTCGATAAAAGAGTATATAGAGGAGACATATCTGTTGCCGAGATTGGATTCTGATGTCTTTTCACCGGAAAAGGTTGGGAGGCAGTGGGACTTTGACTGGTTTGACAAGGCTAATGTACACTTGGAGCCGTCGTTGCCACGGTCTGTTGTCGCTCCGACATGGGAGTTACCGTTTAGGCGCCAGAAGAATGGATCAGAAGGAGGGATTTGGGAACCCAAATCTGTGCAGGTTGATGAAGCAGAACTTACCATAGAAGCACAAGAGTCTGGTTCGTTGCCACGCATGGCCGGACCAGCAAAGGATTTTGTATGGGGAAGCATCAGCAACCGTCCTTTTCGTCCAGGAGGCTTGGATGATTCCCAATCTCTAGAAAGAACTCTTCCTGATGGTGCTTCTAATGGCGAATGGTTACACCAACTTCTTACTGGTGGCCCTGCACAGGCTGTTCCACCAAGTTTTAAGCAAGGATTGGACCTTGGTCCTCTCAAGGCATATCCTGGCTCATGGAATGTATACAATGATCAAGGTTCAGTCAAGAGCACATCAGATGAAAAGCTGAGTGAGTTGTCTGTACAGTTTGATGACTTGTTCAAAAAGGCTTGGGATGAGGACGTTGTTGAGTTTGAAGGAGATGGTCAATCGTCAGGATCGGAATCTGTTAAGTCAGAGGATGAAGCAAATGTGGTTGATGTTGATATTACCAGCAATCCTCCTGAGCCTGAACTGTCTGTGTTGGATGAGATTTTGTCAGTTGAGGCAGGAGAGTCAAAGTCAAGATTTAATGGAACTGGTGGTGAACAGAACCCAGAGGCTTGGGCTATTAGTGGACGTACTGAATGGATTTCGGAGAATTTTCATGATCTTGTTCCTGATAAGGCACTTGATTTTCCCTTTGAATTGGATACATTCCAAAAGGAGGCAATTTATTATCTTGAGAAGGGGGAGTCTGTCTTTGTATCTGCTCACACATCAGCTGGAAAGACAGTAGTTGCAGAATATGCATTTGCTTTGGCATCAAAACATTGCACGAGAGCTGTGTATACTGCTCCTATCAAGACCATCAGCAACCAAAAATATAGGGACTTCTGTGGAAAATTCGATGTTGGACTTCTCACTGGTGATGTTAGCTTGAGGCCCGAGGCTTCTTGTCTCATTATGACCACGGAAATATTAAGGTCAATGCTTTATCGGGGTGCTGACATCATACGTGACATTGAATGGGTTATCTTTGATGAGGTTCATTATGTCAATGATGTCGAAAGAGGTGTTGTTTGGGAAGAAGTTATTATAATGCTCCCGAGACACATCAACATTGTCCTCCTTTCAGCTACGGTACCGAACAAAGTTGAGTTTGCTGACTGGATTGGACGGACAAAGCAAAAGCAAATCCGTGTTACTGGGACTATAAAAAGACCAGTCCCATTGGAGCACTGCCTATTTTACTCCGGAGAACTTTACAAGATATGTGAAAGTGAAAGCTTCATTCCTCAGGGCTTCAAAGCTGCAAAAGATACATTCAAGAAAAAGAATAGTCCTGCCACCAGCGGCGGCGGTGGTGGATCACGTGCTCCAGCTTCTGCTTCAGCTTCTCACGATGGGGCTCGAGGTCAAAAACGTGAAACTTCACATTCAGGAAAACAGAAGCAATTGGGTGCCCATAACTCAGGGAATTTATCCAGAACTGGTGGAGCAAATCAAAACAACGGCAATGGCATGAACAATTGGGGTTTAAGAAGATCAGATGCTTCTTCGTGGTTGTCACTTATTAACAAGCTCTCAAAGAAGTCACTATTACCTGTTGTTATATTTTGTTTCTCAAAGAATCGCTGTGATAGGTCAGCTGATAGCATGTCGGGAATTGACCTCACAAGTAGTTCTGAGAAAAGTCAGATTCGTGTTTTCTGTGACAAAGCATTTTCAAGGCTGAAGGGATCCGACAGGAATTTACCTCAGGTTGTCAGAGTTCAAAACCTTCTCCATAGAGGGATTGGTGTCCATCATGCAGGACTTCTTCCAATTGTTAAGGAAGTTGTTGAAATGCTCTTTTGTCGAGGTGTGATAAAGGTTCTGTTTTCAACAGAGACATTTGCAATGGGAGTGAATGCACCAGCTAGAACGGTTGTCTTTGATACATTAAGGAAATTTGATGGCAAGGAATTTAGACAACTACTACCAGGAGAATACACTCAAATGGCAGGCCGTGCTGGCAGGAGAGGACTTGATAAAATTGGTACAGTTATTGTAATGTGCCGTGACGAAATCCTAGAAGAGAGGGATTTGAAGCATGTCATGGTTGGAAGTGCAACCAGGCTAGAATCTCAGTTTCGGCTTACTTATATTATGATCATGCATCTTCTTCGTGTTGAAGAACTGAAGGTGGAGGACATGCTGAAAAGAAGTTTTGCTGAGTTCCATGCTCAGAAGAAACTACCAGATATGCAGCAACTTCTGATGCGAAAGCTTGCTCAGCCTACAAAAAGTATCGAATGTATAAAAGGGGAACCAGCTATTGAGGAGTATTATGACATGTACTCAGAAAAGGAGAAGCATAAAGCAGAAGTATTGGAGGCAGTAATGCAGTCCCCTGGTGCCCAACAATTTCTTACATCCGGAAGAGTGGTTGTCATGAAATCACAATCACAGGCCCAGAAAAACTTGCTTGGAGTTGTTGTAAAATCACCTTCTTCTAGTAATAAGCAACACATCGTTCTAGTGCTGAAACCTGAACTACCAGCGACAATCCAAACTCCCCTGGCTAGTGGTAACTTACAAGATACCAAAAACACTGATTCTTCTCAGGGTTACTACATGGTAGCGAAATCTAAACGTGGTTTTGAAGAAGAGTATTGTACCTCTGTTTCTTCACGCAAAGGATCAGGTGTCATCAACATAAAATTACCACACCATGGTGCTGCTGCTGGGGTGAGATACGAGGTTAGAGGAGCCGATATCACAGACTTCTCATTCATGTGCAGTAGCAAAATAAAGATTGACCTAGTCCGGCTTCTTGAGGACTGTAGCAGTGCTGCTTACTCCAAGACAGTGCAACTGCTGTTGGACACAAAACCTAAAGGAGAGGAGTACCCTCCAGCCTTAGATCCATTGAAAGAAAAAAAAAATGTGCAGAAGACTTGGATAATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000184 GO:0000288 GO:0000291 GO:0000956 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010035 GO:0010038 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0015672 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0019439 GO:0030001 GO:0032991 GO:0034427 GO:0034641 GO:0034655 GO:0035864 GO:0040029 GO:0042221 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0050789 GO:0050896 GO:0051179 GO:0051234 GO:0055087 GO:0060255 GO:0065007 GO:0070035 GO:0070478 GO:0071704 GO:0090304 GO:0140098 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

DEAD DSHCT Helicase_C rRNA_proc-arch

Protein Analysis

1102

Amino Acids

122.49

Weight (kDa)

6.42

Isoelectric Point (pI)

44.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ski2_N	PF17911	114 - 215	2e-09	Ski2 N-terminal region
DEAD	PF00270	358 - 505	3.9e-23	DEAD/DEAH box helicase
Helicase_C	PF00271	681 - 794	4.5e-06	Helicase conserved C-terminal domain
MTR4-like_stalk	PF21408	849 - 913	1.3e-12	Exosome RNA helicase MTR4-like, stalk
MTR4_beta-barrel	PF13234	917 - 1094	4e-07	Mtr4-like, beta-barrel domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0005110)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46960 AT3G46960 AT3G46960
fragaria_vesca	FvH4_3g37880 FvH4_3g37880 FvH4_3g37880 FvH4_3g37880 FvH4_3g37880
malus_domestica	MD03G1077900.v1.1 MD11G1082500.v1.1
prunus_persica	Prupe.6G062900_v2.0.a1 Prupe.6G062900_v2.0.a1 Prupe.6G062900_v2.0.a1
pyrus_communis	pycom03g06210 pycom11g07060
rosa_chinensis	RchiOBHm_Chr5g0068091
rosa_laevigata	RLG00000035954
rosa_multiflora	Rmu_sc0000435.1_g000017
rosa_roxburghii	Rroxscaffold_1G00012520 Rroxscaffold_1G00013260
rosa_rugosa	Rorug05G0387900 Rorug05G0388300 Rorug05G0388400 Rorug05G0388500
rosa_samantha	Rh5AG446500 Rh5BG464600 Rh5CG486400 Rh5DG478400
rosa_wichuraiana	Rw5G041700 Rw5G041740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1454
Acc36I	ACCTGC	1 cut(s)	382
Acc65I	GGTACC	1 cut(s)	1498
AccB1I	GGYRCC	4 cut(s)	342, 1498, 1817, 2753
AccB7I	CCANNNNNTGG	1 cut(s)	3071
AccI	GTMKAC	2 cut(s)	678, 1205
AccIII	TCCGGA	2 cut(s)	1610, 2774
AciI	CCGC	6 cut(s)	60, 70, 137, 1710, 1713, 1716
AclWI	GGATC	8 cut(s)	12, 364, 820, 1732, 2091, 2104, 3046, 3257
AcoI	YGGCCR	3 cut(s)	52, 61, 450
AcsI	RAATTY	6 cut(s)	1015, 1259, 1834, 2107, 2306, 2321
AcuI	CTGAAG	3 cut(s)	1731, 2111, 2558
AcvI	CACGTG	1 cut(s)	1730
AcyI	GRCGYC	1 cut(s)	343
AfaI	GTAC	8 cut(s)	282, 738, 997, 1500, 2398, 2696, 3016, 3248
AfeI	AGCGCT	1 cut(s)	98
AfiI	CCNNNNNNNGG	3 cut(s)	604, 1081, 3071
AflIII	ACRYGT	1 cut(s)	2691
AjnI	CCWGG	6 cut(s)	505, 661, 2338, 2471, 2749, 2916
AleI	CACNNNNGTG	1 cut(s)	3072
Alw21I	GWGCWC	4 cut(s)	30, 707, 1596, 1735
Alw26I	GTCTC	4 cut(s)	182, 1319, 1459, 2240
AlwI	GGATC	8 cut(s)	12, 364, 820, 1732, 2091, 2104, 3046, 3257
AlwNI	CAGNNNCTG	1 cut(s)	1742
Ama87I	CYCGRG	4 cut(s)	74, 1301, 1462, 1767
Aor13HI	TCCGGA	2 cut(s)	1610, 2774
Aor51HI	AGCGCT	1 cut(s)	98
AoxI	GGCC	7 cut(s)	52, 61, 450, 595, 1298, 2362, 2808
ApeKI	GCWGC	6 cut(s)	17, 1667, 2605, 3077, 3080, 3191
ApoI	RAATTY	6 cut(s)	1015, 1259, 1834, 2107, 2306, 2321
Asp718I	GGTACC	1 cut(s)	1498
AspLEI	GCGC	2 cut(s)	99, 345
AspS9I	GGNCC	6 cut(s)	455, 596, 637, 644, 1299, 2809
AsuC2I	CCSGG	1 cut(s)	38
AsuHPI	GGTGA	5 cut(s)	219, 971, 1295, 2835, 3100
AvaI	CYCGRG	4 cut(s)	74, 1301, 1462, 1767
AvaII	GGWCC	3 cut(s)	455, 637, 644
AxyI	CCTNAGG	2 cut(s)	1653, 2115
BaeGI	GKGCMC	2 cut(s)	1822, 2758
BamHI	GGATCC	2 cut(s)	4, 2096
BanI	GGYRCC	4 cut(s)	342, 1498, 1817, 2753
BanII	GRGCYC	2 cut(s)	30, 1768
BauI	CACGAG	1 cut(s)	1192
BbrPI	CACGTG	1 cut(s)	1730
BbsI	GAAGAC	1 cut(s)	3299
Bbv12I	GWGCWC	4 cut(s)	30, 707, 1596, 1735
BbvI	GCAGC	6 cut(s)	29, 1654, 2617, 3064, 3067, 3178
BccI	CCATC	6 cut(s)	545, 791, 1232, 1754, 2169, 2306
BceAI	ACGGC	4 cut(s)	277, 1885, 2349, 2400
BcgI	CGANNNNNNTGC	2 cut(s)	2504, 2538
BciT130I	CCWGG	6 cut(s)	507, 663, 2340, 2473, 2751, 2918
BciVI	GTATCC	1 cut(s)	1060
BclI	TGATCA	2 cut(s)	685, 2508
BcnI	CCSGG	1 cut(s)	38
BcoDI	GTCTC	4 cut(s)	182, 1319, 1459, 2240
BfaI	CTAG	9 cut(s)	150, 533, 2277, 2429, 2477, 2853, 2876, 2922, 3161
BfmI	CTRYAG	1 cut(s)	3181
BfoI	RGCGCY	2 cut(s)	100, 346
BfuAI	ACCTGC	1 cut(s)	382
BfuI	GTATCC	1 cut(s)	1060
BglI	GCCNNNNNGGC	2 cut(s)	60, 2370
BlpI	GCTNAGC	1 cut(s)	2631
Bme1390I	CCNGG	7 cut(s)	38, 507, 663, 2340, 2473, 2751, 2918
Bme18I	GGWCC	3 cut(s)	455, 637, 644
BmeT110I	CYCGRG	4 cut(s)	74, 1301, 1462, 1767
BmgT120I	GGNCC	6 cut(s)	455, 596, 637, 644, 1299, 2809
BmrFI	CCNGG	7 cut(s)	38, 507, 663, 2340, 2473, 2751, 2918
BmrI	ACTGGG	1 cut(s)	1572
BmsI	GCATC	5 cut(s)	492, 1187, 1897, 2524, 2607
BmuI	ACTGGG	1 cut(s)	1572
BoxI	GACNNNNGTC	1 cut(s)	642
BpiI	GAAGAC	1 cut(s)	3299
BpmI	CTGGAG	2 cut(s)	1719, 3237
Bpu10I	CCTNAGC	1 cut(s)	879
Bpu1102I	GCTNAGC	1 cut(s)	2631
BpuEI	CTTGAG	4 cut(s)	635, 1118, 1315, 3194
BpuMI	CCSGG	1 cut(s)	38
BsaAI	YACGTR	2 cut(s)	1373, 1730
BsaBI	GATNNNNATC	1 cut(s)	817
BsaHI	GRCGYC	1 cut(s)	343
BsaJI	CCNNGG	8 cut(s)	21, 302, 640, 1302, 1326, 2749, 2916, 3070
BsaWI	WCCGGW	3 cut(s)	229, 1610, 2774
BsaXI	ACNNNNNCTCC	6 cut(s)	281, 311, 320, 350, 2820, 2850
Bsc4I	CCNNNNNNNGG	3 cut(s)	604, 1081, 3071
Bse1I	ACTGG	8 cut(s)	266, 595, 958, 1285, 1529, 1567, 1580, 1852
Bse21I	CCTNAGG	2 cut(s)	1653, 2115
Bse3DI	GCAATG	3 cut(s)	1186, 1879, 2262
Bse8I	GATNNNNATC	1 cut(s)	817
BseAI	TCCGGA	2 cut(s)	1610, 2774
BseBI	CCWGG	6 cut(s)	507, 663, 2340, 2473, 2751, 2918
BseDI	CCNNGG	8 cut(s)	21, 302, 640, 1302, 1326, 2749, 2916, 3070
BseGI	GGATG	5 cut(s)	523, 775, 841, 907, 2771
BseJI	GATNNNNATC	1 cut(s)	817
BseLI	CCNNNNNNNGG	3 cut(s)	604, 1081, 3071
BseMI	GCAATG	3 cut(s)	1186, 1879, 2262
BseNI	ACTGG	8 cut(s)	266, 595, 958, 1285, 1529, 1567, 1580, 1852
BseRI	GAGGAG	4 cut(s)	200, 2693, 3120, 3257
BseSI	GKGCMC	2 cut(s)	1822, 2758
BseXI	GCAGC	6 cut(s)	29, 1654, 2617, 3064, 3067, 3178
BseYI	CCCAGC	1 cut(s)	3083
BsgI	GTGCAG	5 cut(s)	70, 410, 585, 3157, 3308
BshFI	GGCC	7 cut(s)	54, 63, 452, 597, 1300, 2364, 2810
BshNI	GGYRCC	4 cut(s)	342, 1498, 1817, 2753
BsiHKAI	GWGCWC	4 cut(s)	30, 707, 1596, 1735
BsiHKCI	CYCGRG	4 cut(s)	74, 1301, 1462, 1767
BsiSI	CCGG	8 cut(s)	37, 51, 161, 230, 453, 1611, 2775, 3166
BslFI	GGGAC	5 cut(s)	266, 1259, 1568, 1579, 2731
BslI	CCNNNNNNNGG	3 cut(s)	604, 1081, 3071
BsmAI	GTCTC	4 cut(s)	182, 1319, 1459, 2240
BsmFI	GGGAC	5 cut(s)	266, 1259, 1568, 1579, 2731
BsmI	GAATGC	1 cut(s)	2272
BsnI	GGCC	7 cut(s)	54, 63, 452, 597, 1300, 2364, 2810
BsoBI	CYCGRG	4 cut(s)	74, 1301, 1462, 1767
Bsp1286I	GDGCHC	7 cut(s)	30, 707, 1596, 1735, 1768, 1822, 2758
Bsp13I	TCCGGA	2 cut(s)	1610, 2774
Bsp1407I	TGTACA	2 cut(s)	280, 736
Bsp1720I	GCTNAGC	1 cut(s)	2631
Bsp19I	CCATGG	1 cut(s)	3070
BspACI	CCGC	6 cut(s)	60, 70, 137, 1710, 1713, 1716
BspANI	GGCC	7 cut(s)	54, 63, 452, 597, 1300, 2364, 2810
BspEI	TCCGGA	2 cut(s)	1610, 2774
BspHI	TCATGA	2 cut(s)	1021, 2790
BspMI	ACCTGC	1 cut(s)	382
BspPI	GGATC	8 cut(s)	12, 364, 820, 1732, 2091, 2104, 3046, 3257
BspT107I	GGYRCC	4 cut(s)	342, 1498, 1817, 2753
BsrDI	GCAATG	3 cut(s)	1186, 1879, 2262
BsrGI	TGTACA	2 cut(s)	280, 736
BsrI	ACTGG	8 cut(s)	266, 595, 958, 1285, 1529, 1567, 1580, 1852
BssECI	CCNNGG	8 cut(s)	21, 302, 640, 1302, 1326, 2749, 2916, 3070
BssNAI	GTATAC	2 cut(s)	679, 1206
BssNI	GRCGYC	1 cut(s)	343
BssSI	CACGAG	1 cut(s)	1192
BssT1I	CCWWGG	3 cut(s)	21, 640, 3070
Bst1107I	GTATAC	2 cut(s)	679, 1206
Bst2BI	CACGAG	1 cut(s)	1192
Bst2UI	CCWGG	6 cut(s)	507, 663, 2340, 2473, 2751, 2918
Bst6I	CTCTTC	4 cut(s)	549, 2427, 2773, 3000
BstACI	GRCGYC	1 cut(s)	343
BstAPI	GCANNNNNTGC	2 cut(s)	1163, 2366
BstAUI	TGTACA	2 cut(s)	280, 736
BstBAI	YACGTR	2 cut(s)	1373, 1730
BstC8I	GCNNGC	4 cut(s)	141, 2362, 2371, 2628
BstDSI	CCRYGG	3 cut(s)	302, 1326, 3070
BstF5I	GGATG	5 cut(s)	523, 775, 841, 907, 2771
BstH2I	RGCGCY	2 cut(s)	100, 346
BstHHI	GCGC	2 cut(s)	99, 345
BstMAI	GTCTC	4 cut(s)	182, 1319, 1459, 2240
BstMWI	GCNNNNNNNGC	9 cut(s)	51, 60, 69, 298, 458, 1163, 2366, 2370, 2632
BstNI	CCWGG	6 cut(s)	507, 663, 2340, 2473, 2751, 2918
BstNSI	RCATGY	4 cut(s)	2017, 2453, 2554, 2695
BstPAI	GACNNNNGTC	1 cut(s)	642
BstSCI	CCNGG	7 cut(s)	36, 505, 661, 2338, 2471, 2749, 2916
BstSFI	CTRYAG	1 cut(s)	3181
BstSLI	GKGCMC	2 cut(s)	1822, 2758
BstV1I	GCAGC	6 cut(s)	29, 1654, 2617, 3064, 3067, 3178
BstV2I	GAAGAC	1 cut(s)	3299
BstX2I	RGATCY	3 cut(s)	4, 2096, 3262
BstXI	CCANNNNNNTGG	2 cut(s)	353, 978
BstYI	RGATCY	3 cut(s)	4, 2096, 3262
BstZ17I	GTATAC	2 cut(s)	679, 1206
Bsu36I	CCTNAGG	2 cut(s)	1653, 2115
BsuI	GTATCC	1 cut(s)	1060
BsuRI	GGCC	7 cut(s)	54, 63, 452, 597, 1300, 2364, 2810
BtgI	CCRYGG	3 cut(s)	302, 1326, 3070
BtsCI	GGATG	5 cut(s)	523, 775, 841, 907, 2771
BtsI	GCAGTG	3 cut(s)	254, 1594, 3193
BtsIMutI	CAGTG	6 cut(s)	254, 1278, 1594, 2949, 3193, 3213
BveI	ACCTGC	1 cut(s)	382
Cac8I	GCNNGC	4 cut(s)	141, 2362, 2371, 2628
CaiI	CAGNNNCTG	1 cut(s)	1742
CciI	TCATGA	2 cut(s)	1021, 2790
CfoI	GCGC	2 cut(s)	99, 345
Cfr13I	GGNCC	6 cut(s)	455, 596, 637, 644, 1299, 2809
Csp6I	GTAC	8 cut(s)	281, 737, 996, 1499, 2397, 2695, 3015, 3247
CviQI	GTAC	8 cut(s)	281, 737, 996, 1499, 2397, 2695, 3015, 3247
DinI	GGCGCC	1 cut(s)	344
EaeI	YGGCCR	3 cut(s)	52, 61, 450
Eam1104I	CTCTTC	4 cut(s)	549, 2427, 2773, 3000
EarI	CTCTTC	4 cut(s)	549, 2427, 2773, 3000
EciI	GGCGGA	1 cut(s)	59
Ecl136II	GAGCTC	1 cut(s)	28
Eco130I	CCWWGG	3 cut(s)	21, 640, 3070
Eco24I	GRGCYC	2 cut(s)	30, 1768
Eco32I	GATATC	1 cut(s)	3115
Eco47I	GGWCC	3 cut(s)	455, 637, 644
Eco47III	AGCGCT	1 cut(s)	98
Eco53kI	GAGCTC	1 cut(s)	28
Eco57I	CTGAAG	3 cut(s)	1731, 2111, 2558
Eco72I	CACGTG	1 cut(s)	1730
Eco81I	CCTNAGG	2 cut(s)	1653, 2115
Eco88I	CYCGRG	4 cut(s)	74, 1301, 1462, 1767
EcoICRI	GAGCTC	1 cut(s)	28
EcoRII	CCWGG	6 cut(s)	505, 661, 2338, 2471, 2749, 2916
EcoRV	GATATC	1 cut(s)	3115
EcoT14I	CCWWGG	3 cut(s)	21, 640, 3070
EcoT22I	ATGCAT	2 cut(s)	1168, 2517
EcoT38I	GRGCYC	2 cut(s)	30, 1768
EgeI	GGCGCC	1 cut(s)	344
EheI	GGCGCC	1 cut(s)	344
ErhI	CCWWGG	3 cut(s)	21, 640, 3070
FalI	AAGNNNNNCTT	2 cut(s)	393, 425
FaqI	GGGAC	5 cut(s)	266, 1259, 1568, 1579, 2731
FbaI	TGATCA	2 cut(s)	685, 2508
FblI	GTMKAC	2 cut(s)	678, 1205
FokI	GGATG	5 cut(s)	530, 782, 848, 914, 2758
FriOI	GRGCYC	2 cut(s)	30, 1768
FspBI	CTAG	9 cut(s)	150, 533, 2277, 2429, 2477, 2853, 2876, 2922, 3161
GlaI	GCGC	2 cut(s)	98, 344
GsaI	CCCAGC	1 cut(s)	3087
GsuI	CTGGAG	2 cut(s)	1719, 3237
HaeII	RGCGCY	2 cut(s)	100, 346
HaeIII	GGCC	7 cut(s)	54, 63, 452, 597, 1300, 2364, 2810
HapII	CCGG	8 cut(s)	37, 51, 161, 230, 453, 1611, 2775, 3166
HhaI	GCGC	2 cut(s)	99, 345
Hin1I	GRCGYC	1 cut(s)	343
Hin6I	GCGC	2 cut(s)	97, 343
HinP1I	GCGC	2 cut(s)	97, 343
HindIII	AAGCTT	2 cut(s)	1642, 2624
HpaII	CCGG	8 cut(s)	37, 51, 161, 230, 453, 1611, 2775, 3166
HphI	GGTGA	5 cut(s)	219, 971, 1295, 2835, 3100
Hpy166II	GTNNAC	5 cut(s)	283, 679, 962, 992, 1206
Hpy8I	GTNNAC	5 cut(s)	283, 679, 962, 992, 1206
Hpy99I	CGWCG	2 cut(s)	95, 298
HpyAV	CCTTC	7 cut(s)	356, 785, 1096, 2086, 2147, 2533, 2856
HpyCH4IV	ACGT	6 cut(s)	777, 994, 1372, 1729, 1780, 2992
HpyF10VI	GCNNNNNNNGC	9 cut(s)	51, 60, 69, 298, 458, 1163, 2366, 2370, 2632
HpySE526I	ACGT	6 cut(s)	777, 994, 1372, 1729, 1780, 2992
Hsp92I	GRCGYC	1 cut(s)	343
HspAI	GCGC	2 cut(s)	97, 343
KasI	GGCGCC	1 cut(s)	342
Kpn2I	TCCGGA	2 cut(s)	1610, 2774
KpnI	GGTACC	1 cut(s)	1502
Ksp22I	TGATCA	2 cut(s)	685, 2508
Lsp1109I	GCAGC	6 cut(s)	29, 1654, 2617, 3064, 3067, 3178
LweI	GCATC	5 cut(s)	492, 1187, 1897, 2524, 2607
MaeI	CTAG	9 cut(s)	150, 533, 2277, 2429, 2477, 2853, 2876, 2922, 3161
MaeII	ACGT	6 cut(s)	777, 994, 1372, 1729, 1780, 2992
MfeI	CAATTG	3 cut(s)	1811, 1885, 2181
MflI	RGATCY	3 cut(s)	4, 2096, 3262
MhlI	GDGCHC	7 cut(s)	30, 707, 1596, 1735, 1768, 1822, 2758
Mly113I	GGCGCC	1 cut(s)	343
MlyI	GAGTC	4 cut(s)	173, 437, 938, 1118
MmeI	TCCRAC	6 cut(s)	344, 879, 1249, 2124, 2440, 3201
Mph1103I	ATGCAT	2 cut(s)	1168, 2517
MroI	TCCGGA	2 cut(s)	1610, 2774
MseI	TTAA	8 cut(s)	624, 825, 945, 1337, 1895, 1932, 2187, 2300
MslI	CAYNNNNRTG	3 cut(s)	2354, 3072, 3133
MspA1I	CMGCKG	4 cut(s)	60, 1139, 1710, 2006
MspI	CCGG	8 cut(s)	37, 51, 161, 230, 453, 1611, 2775, 3166
MspR9I	CCNGG	7 cut(s)	38, 507, 663, 2340, 2473, 2751, 2918
MunI	CAATTG	3 cut(s)	1811, 1885, 2181
Mva1269I	GAATGC	1 cut(s)	2272
MvaI	CCWGG	6 cut(s)	507, 663, 2340, 2473, 2751, 2918
MwoI	GCNNNNNNNGC	9 cut(s)	51, 60, 69, 298, 458, 1163, 2366, 2370, 2632
NarI	GGCGCC	1 cut(s)	343
NciI	CCSGG	1 cut(s)	38
NcoI	CCATGG	1 cut(s)	3070
NmeAIII	GCCGAG	1 cut(s)	228
NmuCI	GTSAC	5 cut(s)	1373, 1923, 1950, 2069, 2417
NsiI	ATGCAT	2 cut(s)	1168, 2517
NspI	RCATGY	4 cut(s)	2017, 2453, 2554, 2695
OliI	CACNNNNGTG	1 cut(s)	3072
PaeR7I	CTCGAG	2 cut(s)	74, 1767
PagI	TCATGA	2 cut(s)	1021, 2790
PciI	ACATGT	1 cut(s)	2691
PcsI	WCGNNNNNNNCGW	1 cut(s)	164
PctI	GAATGC	1 cut(s)	2272
PfeI	GAWTC	7 cut(s)	212, 521, 818, 1987, 2056, 2480, 2954
PflFI	GACNNNGTC	1 cut(s)	3161
PflMI	CCANNNNNTGG	1 cut(s)	3071
PfoI	TCCNGGA	1 cut(s)	505
PleI	GAGTC	4 cut(s)	172, 436, 937, 1117
PluTI	GGCGCC	1 cut(s)	346
PmaCI	CACGTG	1 cut(s)	1730
PmlI	CACGTG	1 cut(s)	1730
PpsI	GAGTC	4 cut(s)	172, 436, 937, 1117
Ppu21I	YACGTR	2 cut(s)	1373, 1730
PscI	ACATGT	1 cut(s)	2691
PshAI	GACNNNNGTC	1 cut(s)	642
PsiI	TTATAA	1 cut(s)	1454
Psp124BI	GAGCTC	1 cut(s)	30
Psp6I	CCWGG	6 cut(s)	505, 661, 2338, 2471, 2749, 2916
PspCI	CACGTG	1 cut(s)	1730
PspFI	CCCAGC	1 cut(s)	3083
PspGI	CCWGG	6 cut(s)	505, 661, 2338, 2471, 2749, 2916
PspPI	GGNCC	6 cut(s)	455, 596, 637, 644, 1299, 2809
PspXI	VCTCGAGB	2 cut(s)	74, 1767
PstNI	CAGNNNCTG	1 cut(s)	1742
PsuI	RGATCY	3 cut(s)	4, 2096, 3262
PsyI	GACNNNGTC	1 cut(s)	3161
PvuII	CAGCTG	2 cut(s)	1139, 2006
RsaI	GTAC	8 cut(s)	282, 738, 997, 1500, 2398, 2696, 3016, 3248
RsaNI	GTAC	8 cut(s)	281, 737, 996, 1499, 2397, 2695, 3015, 3247
RseI	CAYNNNNRTG	3 cut(s)	2354, 3072, 3133
SacI	GAGCTC	1 cut(s)	30
SaqAI	TTAA	8 cut(s)	624, 825, 945, 1337, 1895, 1932, 2187, 2300
Sau96I	GGNCC	6 cut(s)	455, 596, 637, 644, 1299, 2809
SchI	GAGTC	4 cut(s)	173, 437, 938, 1118
ScrFI	CCNGG	7 cut(s)	38, 507, 663, 2340, 2473, 2751, 2918
SduI	GDGCHC	7 cut(s)	30, 707, 1596, 1735, 1768, 1822, 2758
SfaNI	GCATC	5 cut(s)	492, 1187, 1897, 2524, 2607
SfcI	CTRYAG	1 cut(s)	3181
SfiI	GGCCNNNNNGGCC	1 cut(s)	60
SfoI	GGCGCC	1 cut(s)	344
Sfr274I	CTCGAG	2 cut(s)	74, 1767
SinI	GGWCC	3 cut(s)	455, 637, 644
SlaI	CTCGAG	2 cut(s)	74, 1767
SmiMI	CAYNNNNRTG	3 cut(s)	2354, 3072, 3133
SmlI	CTYRAG	6 cut(s)	74, 650, 1097, 1294, 1767, 3173
SmoI	CTYRAG	6 cut(s)	74, 650, 1097, 1294, 1767, 3173
SsiI	CCGC	6 cut(s)	60, 70, 137, 1710, 1713, 1716
SspDI	GGCGCC	1 cut(s)	342
SspI	AATATT	1 cut(s)	1335
SspMI	CTAG	9 cut(s)	150, 533, 2277, 2429, 2477, 2853, 2876, 2922, 3161
SstI	GAGCTC	1 cut(s)	30
StyD4I	CCNGG	7 cut(s)	36, 505, 661, 2338, 2471, 2749, 2916
StyI	CCWWGG	3 cut(s)	21, 640, 3070
TaiI	ACGT	6 cut(s)	780, 997, 1375, 1732, 1783, 2995
TaqI	TCGA	8 cut(s)	75, 93, 167, 1263, 1422, 1768, 2215, 2649
TatI	WGTACW	3 cut(s)	280, 736, 2694
TauI	GCSGC	4 cut(s)	63, 139, 1713, 1716
TfiI	GAWTC	7 cut(s)	212, 521, 818, 1987, 2056, 2480, 2954
Tru1I	TTAA	8 cut(s)	624, 825, 945, 1337, 1895, 1932, 2187, 2300
Tru9I	TTAA	8 cut(s)	624, 825, 945, 1337, 1895, 1932, 2187, 2300
TscAI	CASTG	6 cut(s)	254, 1285, 1601, 2956, 3193, 3213
TseFI	GTSAC	5 cut(s)	1373, 1923, 1950, 2069, 2417
TseI	GCWGC	6 cut(s)	17, 1667, 2605, 3077, 3080, 3191
Tsp45I	GTSAC	5 cut(s)	1373, 1923, 1950, 2069, 2417
TspDTI	ATGAA	9 cut(s)	414, 729, 852, 1010, 1394, 1636, 1895, 2807, 3121
TspGWI	ACGGA	3 cut(s)	1343, 1544, 1549
TspRI	CASTG	6 cut(s)	254, 1285, 1601, 2956, 3193, 3213
Tth111I	GACNNNGTC	1 cut(s)	3161
Van91I	CCANNNNNTGG	1 cut(s)	3071
VpaK11BI	GGWCC	3 cut(s)	455, 637, 644
XapI	RAATTY	6 cut(s)	1015, 1259, 1834, 2107, 2306, 2321
XbaI	TCTAGA	1 cut(s)	532
XceI	RCATGY	4 cut(s)	2017, 2453, 2554, 2695
XcmI	CCANNNNNNNNNTGG	2 cut(s)	587, 2150
XhoI	CTCGAG	2 cut(s)	74, 1767
XmiI	GTMKAC	2 cut(s)	678, 1205
XspI	CTAG	9 cut(s)	150, 533, 2277, 2429, 2477, 2853, 2876, 2922, 3161
Zsp2I	ATGCAT	2 cut(s)	1168, 2517

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.