Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0005110

Physical Location & Seq

Genomic Coordinates Reverse (-)

74152477 .. 74165941

Gene Structure 13465 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ34373

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4059 bp

ATGGATCCGATACAAGCAGCCAAGGAGCTCTCATTCCGGGTCGGCTTCTCCGGCCACAGCGGCCACCTCCGCCTCGAGCCTCTCTGCACCGTCGAGCGCTCCGACCCCGTCAAGTCGCTCCCCGATTTCGTTCTGCCGCCTGCATTTGCTAGGGAAACGCCGGAGTCGATAAAAGAGTATATAGAGGAGACATATCTATTGCCAAGATTGGATTCTGATGTATTTTCACCGGAAAAGGTTGGGAGGCAGTGGGATTTTGACTGGTTTGACAAGGCTAATGTACAGTTGGAGCCGTCATTGCCGCGGTCTGTTGTTGTTCCCACATGGGAGTTACCGTTTAGGCGCCAGAAGAATGGATCAGAAGGAGGGATTTGGGAACCCAAATCTGTGCAGGTCGATGAAGCAGAACTTACCATAGAAGCACAAGAGTCTGGTTCGTTGCCACGCATGGCCGGACCAGCAAAGGATTTTGTATGGGGAAGCATCAGCAACCGTCCTTTTCGTCCAGGAGGCTTGGATGATTCCCAATCTCTAGAAAGAACTCTTCCTGATGGTGCTTCTAATGGCGAATGGGTACACGAACTTCTTACTGGTGGCCCTGCACAGGCTGTTCCACCAAGTTTTAAGCAAGGCTTGGACCTTGGTCCTCTCAAGGCATATCCTGGCTCATGGAATGTATACAATGATCAAGGTTCAGTCAAGAGCACATCAGATGAAAAGCTGGGTTTGCAGAGTGAGTTGTCTGTACAGTTTGATGACTTGTTCAAAAAGGCTTGGGATGAGGATGTTGTTGAGTTTGAAGGAAATGGTCAATCGTCAGGATCGGAATCTGTTAAGTCAGAGGATGAAGCAAATGTTGTTGATGTTGATATTACCAGCAATTCTCCTGAGCCTGAACTGTCTGTGTTGGATGAGATTTTGTCAGTTGAGGCAGGAGACTCAAAGTCAAGATTTAATGGAACTAGTGGTGACCCGGAGGCCTGGGCTATTAGTGGACATACTGAATGGATTTCGGAGAATTTTCATGATCTTGTTCCTGATAAGGCACTTGATTTCCCTTTTGAATTGGATACATTCCAAAAGGAGGCAATTTATTATCTTGAGAAGGGGGAGTCTGTCTTTGTAGCTGCTCACACATCAGCTGGAAAGACAGTTGTTGCAGAATATGCATTTGCTCTGGCATCAAAACATTGCACGAGAGCTGTGTATACTGCTCCTATCAAAACCATCAGCAACCAAAAATATAGGGACTTCTGTGGAAAATTCGATGTCGGACTTCTCACTGGTGATGTTAGCTTGAGGCCCGAGGCTTCTTGTCTCATTATGACCACGGAAATATTAAGGTCAATGCTTTATCGGGGTGCTGACATCATACGTGATATTGAATGGGTTATCTTTGATGAGGTTCATTATGTCAATGATGTCGAAAGAGGTGTTGTTTGGGAAGAAGTTATTATAATGCTCCCGAGACACATCAACATTGTCCTCCTTTCAGCTACGGTACCAAACAAAGTTGAGTTTGCTGACTGGATTGGACGGACAAAGCAAAAGCAAATCCGTGTTACAGGGACTACAAAAAGACCAGTCCCATTGGAGCACTGCCTATTTTACTCTGGAGAACTTTACAAGATATGTGAAAGTGAAAGCTTCATTCCTCAGGGCTTTAAAGCTGCAAAAGATACATTCAAAAAAAAGAATATGAGTCCTGCCACCGGCGGTGGCGGCGGCGGATCACGTGCTCCAGCTTCTCATGATGCGGCTCGCGGTCAAAAACGTGAAACTTCACACTCGGGAAAACAGAAGCAATCGGGTGCCCATAATTCAGGGAATTTATCCAGAACTGGTGGGGCAAATCAAAACAACGGCAATGGCATGAACAATTGGGGTTTAAGAAGATCAGATGCTTCTTCGTGGTTGTCACTTATTAACAAGCTCTCAAAGAAGTCACTATTACCTGTGGTTATATTTTGTTTCTCAAAGAATCGCTGTGATAGGTCAGCTGATAGCATGCCGGGAACTGACCTGACAAGTAGTTCTGAGAAAAGTCAGATTCGTGTTTTCTGTGACAAAGCATTTTCACGGCTGAAGGGATCCGATAGGAATTTACCTCAGGTTCTCAGAGTTCAAAACCTTCTCCATAGAGGGATTGGTGTCCATCATGCAGGACTTCTTCCAATTGTTAAGGAAGTTGTTGAAATGCTCTTTTGTCGCGGTGTGATAAAGGTTCTGTTTTCAACAGAAACATTTGCAATGGGAGTGAATGCACCTGCTAGAACGGTTGTCTTTGATACATTAAGGAAATTTGATGGCAAGGAATTTAGACAACTACTACCAGGAGAATATACTCAAATGGCAGGCCGTGCTGGCAGGAGAGGACTTGATAAAATTGGTACAGTTATTGTAATGTGCCGTGATGAAATCCTAGAAGAGAGGGATTTGAAGCATGTCATAGTTGGAAGTGCAACCAGGCTAGAATCTCAGTTTCGGCTTACTTATATTATGATCATGCATCTTCTTCGTGTTGAAGAACTGAAGGTGGAGGACATGCTGAAAAGAAGTTTTGCTGAGTTCCATGCTCAGAAGAAACTACCAGATATGCAGCAACTTCTGATGCGAAAGCTTGCTCAGCCTACAAAAAGTATCGAATGTATAAAAGGGGAACCAGCTATTGAGGAGTATTATGACATATACTTAGAAGCAGAGACGCATGAAACAGAAATATTGGAGGCAGTAATGCAGTCCCCTGTTGCCCAACAATTTCTTACACCTGGAAGAGTGGTTGTCATGAAATCACAATCAGCCCAGGATCACTTGCTTGGAGTTGTTGTGAAAGCACCTTCTTCTAGTAATAAGCAACACATCGTTCTAGTGCTGAAACCTGAATTACCAGCGACAATCCAAACTCCCCTGGCTAGTGGTAACTTGCAAGATACCAAAAACACTGATTCTTCTCAGGGTTACTACATGGTACTGAAATCTAAACGTGCTCTTGAAGAAGAGTATTGCACCTCTGTTTCTTCACGCAAAGGATCAGGTGTTATCAACATAAAATTACCACACCATGGTGCTGCTGCTGGGGTGAGATACGAGGTTAGAGGAGCCGATAACACAGACTTCTCATACATATGCACTCGCAAAATAAAGATTGACCAAATTCGGCTTCTTGAGGACGGTAGCAGTGCTGCTTACTCCAAGACAGTGCAACAGCTGTTGGACACAAAATCTGATGGAAATAAGTACCCTCCAGCCTTAGATCCATTAAAAGATCTGAAGTTGAAAGACATGCATCTTGTGGAAAAATACTACAGATGGACAAACTTGTTGCAGCAGATGGCAAAGAATAAGTGCCATGGATGTATTAAATTGGAGGAACACATTAAGTTAGCAAGAGAGATAAAGAGACACAGAGAGGAAGTTAATGCTCTTCAATTTCAAATGTCAGACGAAGCACTTCAACAGATGCCAGATTTTCAAGGACGGATAGATGTTCTGAAGGAAATCGGATGTATAGATGCTGACCTTGTAGTTCAAATAAAAGGCCGTGTCGCATGTGAGATGAATTCTGGGGAGGAGTTGATCTGCACAGAGTGTTTGTTTGAGAACCAACTAGATGACCTGGAACCAGAAGAAGCGGTGGCATTAATGTCATCCTTCGTTTTCCAGCAGAAGAATACTTCTGAACCTTCGCTTACTCCTAAACTTTCTATGGCGAAAGAGAGATTGTACAATACAGCAATAAGACTAGGCGAGTGTCAAGCTTACTTCAAACTTCCAATAAATCCAGAAGAGTACGCCAAAGAGAATCTCAAGTTTGGTCTTGTTGAAGTTGTTTATGAGTGGGCAAAGGGCACACCATTTGCAGATATTTGTGAACTCACAGATGTTCCTGAAGGCATGATAGTGCGGACCATTGTTAGACTTGATGAGACGTGTCGTGAATTCAAAAATGCTGCATCTATTATGGGTAATTCTGCTCTATACAAGAAAATGGAGACAGCTTCAAATGCAATAAAACGTGACATTGTTTTCGCAGCTAGCTTGTACGTAACAGGAGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000184 GO:0000288 GO:0000291 GO:0000956 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010035 GO:0010038 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0015672 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0019439 GO:0030001 GO:0032991 GO:0034427 GO:0034641 GO:0034655 GO:0035864 GO:0040029 GO:0042221 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0050789 GO:0050896 GO:0051179 GO:0051234 GO:0055087 GO:0060255 GO:0065007 GO:0070035 GO:0070478 GO:0071704 GO:0090304 GO:0140098 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

DEAD DSHCT Helicase_C rRNA_proc-arch

Protein Analysis

1352

Amino Acids

150.96

Weight (kDa)

5.76

Isoelectric Point (pI)

46.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ski2_N	PF17911	114 - 215	5.8e-09	Ski2 N-terminal region
DEAD	PF00270	359 - 506	6e-23	DEAD/DEAH box helicase
Helicase_C	PF00271	679 - 792	3.9e-06	Helicase conserved C-terminal domain
MTR4-like_stalk	PF21408	847 - 911	1.3e-12	Exosome RNA helicase MTR4-like, stalk
MTR4_beta-barrel	PF13234	914 - 1148	1.6e-25	Mtr4-like, beta-barrel domain
DSHCT	PF08148	1174 - 1344	2.2e-58	DSHCT (NUC185) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005110)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46960 AT3G46960 AT3G46960
fragaria_vesca	FvH4_3g37880 FvH4_3g37880 FvH4_3g37880 FvH4_3g37880 FvH4_3g37880
malus_domestica	MD03G1077900.v1.1 MD11G1082500.v1.1
prunus_persica	Prupe.6G062900_v2.0.a1 Prupe.6G062900_v2.0.a1 Prupe.6G062900_v2.0.a1
pyrus_communis	pycom03g06210 pycom11g07060
rosa_chinensis	RchiOBHm_Chr5g0068091
rosa_laevigata	RLG00000035954
rosa_multiflora	Rmu_sc0000435.1_g000017
rosa_roxburghii	Rroxscaffold_1G00012520 Rroxscaffold_1G00013260
rosa_rugosa	Rorug05G0387900 Rorug05G0388300 Rorug05G0388400 Rorug05G0388500
rosa_samantha	Rh5AG446500 Rh5BG464600 Rh5CG486400 Rh5DG478400
rosa_wichuraiana	Rw5G041700 Rw5G041740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1457
AarI	CACCTGC	1 cut(s)	2275
Acc36I	ACCTGC	2 cut(s)	382, 2275
Acc65I	GGTACC	1 cut(s)	1501
AccB1I	GGYRCC	3 cut(s)	342, 1501, 1811
AccB7I	CCANNNNNTGG	1 cut(s)	3062
AccI	GTMKAC	2 cut(s)	678, 1208
AccII	CGCG	3 cut(s)	304, 1764, 2211
AclWI	GGATC	9 cut(s)	12, 364, 829, 1738, 2085, 2098, 2814, 3037, 3248
AcoI	YGGCCR	3 cut(s)	52, 61, 450
AcsI	RAATTY	9 cut(s)	1018, 1262, 1828, 2101, 2300, 2315, 3153, 3559, 3938
AcuI	CTGAAG	5 cut(s)	2105, 2552, 3290, 3512, 3909
AcvI	CACGTG	1 cut(s)	1736
AcyI	GRCGYC	1 cut(s)	343
AdeI	CACNNNGTG	1 cut(s)	3588
AfeI	AGCGCT	1 cut(s)	98
AfiI	CCNNNNNNNGG	4 cut(s)	604, 1084, 1712, 3062
AflIII	ACRYGT	1 cut(s)	3929
AhdI	GACNNNNNGTC	1 cut(s)	943
AhlI	ACTAGT	1 cut(s)	962
AjiI	CACGTC	1 cut(s)	3930
AjnI	CCWGG	9 cut(s)	505, 661, 980, 2332, 2465, 2767, 2802, 2907, 3615
AjuI	GAANNNNNNNTTGG	4 cut(s)	196, 228, 3144, 3176
AleI	CACNNNNGTG	1 cut(s)	3063
Alw21I	GWGCWC	5 cut(s)	30, 707, 1599, 1741, 2989
Alw26I	GTCTC	8 cut(s)	182, 930, 1322, 1462, 2696, 3394, 3920, 3986
AlwI	GGATC	9 cut(s)	12, 364, 829, 1738, 2085, 2098, 2814, 3037, 3248
Ama87I	CYCGRG	4 cut(s)	74, 1304, 1465, 1789
Aor51HI	AGCGCT	1 cut(s)	98
AoxI	GGCC	8 cut(s)	52, 61, 450, 595, 978, 1301, 2356, 3538
ApoI	RAATTY	9 cut(s)	1018, 1262, 1828, 2101, 2300, 2315, 3153, 3559, 3938
ArsI	GACNNNNNNTTYG	2 cut(s)	4010, 4042
AseI	ATTAAT	1 cut(s)	3641
Asp700I	GAANNNNTTC	1 cut(s)	3450
Asp718I	GGTACC	1 cut(s)	1501
AspLEI	GCGC	2 cut(s)	99, 345
AspS9I	GGNCC	6 cut(s)	455, 596, 637, 644, 1302, 3906
AsuC2I	CCSGG	3 cut(s)	38, 974, 2013
AsuHPI	GGTGA	4 cut(s)	219, 980, 1298, 3091
AsuNHI	GCTAGC	1 cut(s)	4034
AvaI	CYCGRG	4 cut(s)	74, 1304, 1465, 1789
AvaII	GGWCC	4 cut(s)	455, 637, 644, 3906
AxyI	CCTNAGG	2 cut(s)	1656, 2109
BaeGI	GKGCMC	2 cut(s)	1816, 3851
BamHI	GGATCC	2 cut(s)	4, 2090
BanI	GGYRCC	3 cut(s)	342, 1501, 1811
BanII	GRGCYC	1 cut(s)	30
BarI	GAAGNNNNNNTAC	2 cut(s)	567, 599
BauI	CACGAG	1 cut(s)	1195
BbrPI	CACGTG	1 cut(s)	1736
Bbv12I	GWGCWC	5 cut(s)	30, 707, 1599, 1741, 2989
BccI	CCATC	7 cut(s)	545, 1235, 2163, 2300, 3221, 3303, 3325
BceAI	ACGGC	6 cut(s)	277, 1879, 2096, 2343, 2394, 3525
BcgI	CGANNNNNNTGC	2 cut(s)	2498, 2532
BciT130I	CCWGG	9 cut(s)	507, 663, 982, 2334, 2467, 2769, 2804, 2909, 3617
BciVI	GTATCC	1 cut(s)	1063
BclI	TGATCA	2 cut(s)	685, 2502
BcnI	CCSGG	3 cut(s)	38, 974, 2013
BcoDI	GTCTC	8 cut(s)	182, 930, 1322, 1462, 2696, 3394, 3920, 3986
BcuI	ACTAGT	1 cut(s)	962
BfmI	CTRYAG	1 cut(s)	3304
BfoI	RGCGCY	2 cut(s)	100, 346
BfuAI	ACCTGC	2 cut(s)	382, 2275
BfuI	GTATCC	1 cut(s)	1063
BglI	GCCNNNNNGGC	2 cut(s)	60, 2364
BglII	AGATCT	1 cut(s)	3265
BlpI	GCTNAGC	1 cut(s)	2625
Bme18I	GGWCC	4 cut(s)	455, 637, 644, 3906
BmeRI	GACNNNNNGTC	1 cut(s)	943
BmeT110I	CYCGRG	4 cut(s)	74, 1304, 1465, 1789
BmgBI	CACGTC	1 cut(s)	3930
BmgT120I	GGNCC	6 cut(s)	455, 596, 637, 644, 1302, 3906
BmtI	GCTAGC	1 cut(s)	4038
BoxI	GACNNNNGTC	1 cut(s)	642
BpmI	CTGGAG	3 cut(s)	1635, 1725, 3228
Bpu10I	CCTNAGC	1 cut(s)	888
Bpu1102I	GCTNAGC	1 cut(s)	2625
BpuEI	CTTGAG	5 cut(s)	635, 1121, 1318, 3185, 3791
BpuMI	CCSGG	3 cut(s)	38, 974, 2013
BsaAI	YACGTR	3 cut(s)	1376, 1736, 4045
BsaBI	GATNNNNATC	1 cut(s)	826
BsaHI	GRCGYC	1 cut(s)	343
BsaWI	WCCGGW	1 cut(s)	229
BsaXI	ACNNNNNCTCC	4 cut(s)	320, 350, 2811, 2841
Bsc4I	CCNNNNNNNGG	4 cut(s)	604, 1084, 1712, 3062
Bse118I	RCCGGY	1 cut(s)	1712
Bse1I	ACTGG	6 cut(s)	266, 595, 1288, 1532, 1583, 1846
Bse21I	CCTNAGG	2 cut(s)	1656, 2109
Bse3DI	GCAATG	4 cut(s)	296, 1189, 1873, 2256
Bse8I	GATNNNNATC	1 cut(s)	826
BseBI	CCWGG	9 cut(s)	507, 663, 982, 2334, 2467, 2769, 2804, 2909, 3617
BseGI	GGATG	8 cut(s)	523, 784, 790, 850, 916, 3359, 3509, 3647
BseJI	GATNNNNATC	1 cut(s)	826
BseLI	CCNNNNNNNGG	4 cut(s)	604, 1084, 1712, 3062
BseMI	GCAATG	4 cut(s)	296, 1189, 1873, 2256
BseNI	ACTGG	6 cut(s)	266, 595, 1288, 1532, 1583, 1846
BseRI	GAGGAG	4 cut(s)	200, 2687, 3111, 3584
BseSI	GKGCMC	2 cut(s)	1816, 3851
BseYI	CCCAGC	2 cut(s)	721, 3074
BsgI	GTGCAG	4 cut(s)	70, 410, 585, 3565
Bsh1236I	CGCG	3 cut(s)	304, 1764, 2211
BshFI	GGCC	8 cut(s)	54, 63, 452, 597, 980, 1303, 2358, 3540
BshNI	GGYRCC	3 cut(s)	342, 1501, 1811
BsiHKAI	GWGCWC	5 cut(s)	30, 707, 1599, 1741, 2989
BsiHKCI	CYCGRG	4 cut(s)	74, 1304, 1465, 1789
BsiSI	CCGG	8 cut(s)	37, 51, 161, 230, 453, 974, 1713, 2012
BslFI	GGGAC	4 cut(s)	1262, 1571, 1582, 2725
BslI	CCNNNNNNNGG	4 cut(s)	604, 1084, 1712, 3062
BsmAI	GTCTC	8 cut(s)	182, 930, 1322, 1462, 2696, 3394, 3920, 3986
BsmBI	CGTCTC	2 cut(s)	2696, 3920
BsmFI	GGGAC	4 cut(s)	1262, 1571, 1582, 2725
BsmI	GAATGC	1 cut(s)	2266
BsnI	GGCC	8 cut(s)	54, 63, 452, 597, 980, 1303, 2358, 3540
BsoBI	CYCGRG	4 cut(s)	74, 1304, 1465, 1789
Bsp1286I	GDGCHC	7 cut(s)	30, 707, 1599, 1741, 1816, 2989, 3851
Bsp1407I	TGTACA	3 cut(s)	280, 745, 3723
Bsp1720I	GCTNAGC	1 cut(s)	2625
Bsp19I	CCATGG	2 cut(s)	3061, 3349
BspANI	GGCC	8 cut(s)	54, 63, 452, 597, 980, 1303, 2358, 3540
BspFNI	CGCG	3 cut(s)	304, 1764, 2211
BspHI	TCATGA	3 cut(s)	1024, 1750, 2784
BspMI	ACCTGC	2 cut(s)	382, 2275
BspOI	GCTAGC	1 cut(s)	4038
BspPI	GGATC	9 cut(s)	12, 364, 829, 1738, 2085, 2098, 2814, 3037, 3248
BspQI	GCTCTTC	1 cut(s)	3429
BspT107I	GGYRCC	3 cut(s)	342, 1501, 1811
BsrDI	GCAATG	4 cut(s)	296, 1189, 1873, 2256
BsrFI	RCCGGY	1 cut(s)	1712
BsrGI	TGTACA	3 cut(s)	280, 745, 3723
BsrI	ACTGG	6 cut(s)	266, 595, 1288, 1532, 1583, 1846
BssAI	RCCGGY	1 cut(s)	1712
BssNAI	GTATAC	2 cut(s)	679, 1209
BssNI	GRCGYC	1 cut(s)	343
BssSI	CACGAG	1 cut(s)	1195
BssT1I	CCWWGG	4 cut(s)	21, 640, 3061, 3349
Bst1107I	GTATAC	2 cut(s)	679, 1209
Bst2BI	CACGAG	1 cut(s)	1195
Bst2UI	CCWGG	9 cut(s)	507, 663, 982, 2334, 2467, 2769, 2804, 2909, 3617
Bst6I	CTCTTC	6 cut(s)	549, 2421, 2767, 2991, 3429, 3780
BstACI	GRCGYC	1 cut(s)	343
BstAPI	GCANNNNNTGC	2 cut(s)	1166, 2360
BstAUI	TGTACA	3 cut(s)	280, 745, 3723
BstBAI	YACGTR	3 cut(s)	1376, 1736, 4045
BstC8I	GCNNGC	7 cut(s)	141, 1762, 2009, 2356, 2365, 2622, 4036
BstDSI	CCRYGG	4 cut(s)	302, 1329, 3061, 3349
BstEII	GGTNACC	1 cut(s)	968
BstF5I	GGATG	8 cut(s)	523, 784, 790, 850, 916, 3359, 3509, 3647
BstFNI	CGCG	3 cut(s)	304, 1764, 2211
BstH2I	RGCGCY	2 cut(s)	100, 346
BstHHI	GCGC	2 cut(s)	99, 345
BstMAI	GTCTC	8 cut(s)	182, 930, 1322, 1462, 2696, 3394, 3920, 3986
BstNI	CCWGG	9 cut(s)	507, 663, 982, 2334, 2467, 2769, 2804, 2909, 3617
BstNSI	RCATGY	5 cut(s)	2011, 2447, 2548, 3286, 3552
BstPAI	GACNNNNGTC	1 cut(s)	642
BstPI	GGTNACC	1 cut(s)	968
BstSFI	CTRYAG	1 cut(s)	3304
BstSLI	GKGCMC	2 cut(s)	1816, 3851
BstSNI	TACGTA	1 cut(s)	4045
BstUI	CGCG	3 cut(s)	304, 1764, 2211
BstX2I	RGATCY	4 cut(s)	4, 2090, 3253, 3265
BstXI	CCANNNNNNTGG	1 cut(s)	353
BstYI	RGATCY	4 cut(s)	4, 2090, 3253, 3265
BstZ17I	GTATAC	2 cut(s)	679, 1209
Bsu36I	CCTNAGG	2 cut(s)	1656, 2109
BsuI	GTATCC	1 cut(s)	1063
BsuRI	GGCC	8 cut(s)	54, 63, 452, 597, 980, 1303, 2358, 3540
BtgI	CCRYGG	4 cut(s)	302, 1329, 3061, 3349
BtrI	CACGTC	1 cut(s)	3930
BtsCI	GGATG	8 cut(s)	523, 784, 790, 850, 916, 3359, 3509, 3647
BtsI	GCAGTG	3 cut(s)	254, 1597, 3184
BtsIMutI	CAGTG	6 cut(s)	254, 1281, 1597, 2940, 3184, 3204
BveI	ACCTGC	2 cut(s)	382, 2275
Cac8I	GCNNGC	7 cut(s)	141, 1762, 2009, 2356, 2365, 2622, 4036
CciI	TCATGA	3 cut(s)	1024, 1750, 2784
CfoI	GCGC	2 cut(s)	99, 345
Cfr10I	RCCGGY	1 cut(s)	1712
Cfr13I	GGNCC	6 cut(s)	455, 596, 637, 644, 1302, 3906
Cfr42I	CCGCGG	1 cut(s)	305
CseI	GACGC	1 cut(s)	2713
DinI	GGCGCC	1 cut(s)	344
DraI	TTTAAA	1 cut(s)	1666
DraIII	CACNNNGTG	1 cut(s)	3588
DriI	GACNNNNNGTC	1 cut(s)	943
EaeI	YGGCCR	3 cut(s)	52, 61, 450
Eam1104I	CTCTTC	6 cut(s)	549, 2421, 2767, 2991, 3429, 3780
Eam1105I	GACNNNNNGTC	1 cut(s)	943
EarI	CTCTTC	6 cut(s)	549, 2421, 2767, 2991, 3429, 3780
EciI	GGCGGA	2 cut(s)	59, 1743
Ecl136II	GAGCTC	1 cut(s)	28
Eco105I	TACGTA	1 cut(s)	4045
Eco130I	CCWWGG	4 cut(s)	21, 640, 3061, 3349
Eco147I	AGGCCT	1 cut(s)	980
Eco24I	GRGCYC	1 cut(s)	30
Eco47I	GGWCC	4 cut(s)	455, 637, 644, 3906
Eco47III	AGCGCT	1 cut(s)	98
Eco53kI	GAGCTC	1 cut(s)	28
Eco57I	CTGAAG	5 cut(s)	2105, 2552, 3290, 3512, 3909
Eco72I	CACGTG	1 cut(s)	1736
Eco81I	CCTNAGG	2 cut(s)	1656, 2109
Eco88I	CYCGRG	4 cut(s)	74, 1304, 1465, 1789
Eco91I	GGTNACC	1 cut(s)	968
EcoICRI	GAGCTC	1 cut(s)	28
EcoO65I	GGTNACC	1 cut(s)	968
EcoRI	GAATTC	2 cut(s)	3559, 3938
EcoRII	CCWGG	9 cut(s)	505, 661, 980, 2332, 2465, 2767, 2802, 2907, 3615
EcoT14I	CCWWGG	4 cut(s)	21, 640, 3061, 3349
EcoT22I	ATGCAT	3 cut(s)	1171, 2511, 3288
EcoT38I	GRGCYC	1 cut(s)	30
EgeI	GGCGCC	1 cut(s)	344
EheI	GGCGCC	1 cut(s)	344
ErhI	CCWWGG	4 cut(s)	21, 640, 3061, 3349
Esp3I	CGTCTC	2 cut(s)	2696, 3920
FalI	AAGNNNNNCTT	4 cut(s)	393, 425, 617, 649
FaqI	GGGAC	4 cut(s)	1262, 1571, 1582, 2725
FauNDI	CATATG	1 cut(s)	3125
FbaI	TGATCA	2 cut(s)	685, 2502
FblI	GTMKAC	2 cut(s)	678, 1208
FokI	GGATG	8 cut(s)	530, 791, 797, 857, 923, 3366, 3516, 3634
FriOI	GRGCYC	1 cut(s)	30
GlaI	GCGC	2 cut(s)	98, 344
GsaI	CCCAGC	2 cut(s)	725, 3078
GsuI	CTGGAG	3 cut(s)	1635, 1725, 3228
HaeII	RGCGCY	2 cut(s)	100, 346
HaeIII	GGCC	8 cut(s)	54, 63, 452, 597, 980, 1303, 2358, 3540
HapII	CCGG	8 cut(s)	37, 51, 161, 230, 453, 974, 1713, 2012
HgaI	GACGC	1 cut(s)	2713
HhaI	GCGC	2 cut(s)	99, 345
Hin1I	GRCGYC	1 cut(s)	343
Hin6I	GCGC	2 cut(s)	97, 343
HinP1I	GCGC	2 cut(s)	97, 343
HindIII	AAGCTT	3 cut(s)	1645, 2618, 3756
HpaII	CCGG	8 cut(s)	37, 51, 161, 230, 453, 974, 1713, 2012
HphI	GGTGA	4 cut(s)	219, 980, 1298, 3091
Hpy166II	GTNNAC	5 cut(s)	577, 679, 995, 1209, 3872
Hpy8I	GTNNAC	5 cut(s)	577, 679, 995, 1209, 3872
Hpy99I	CGWCG	1 cut(s)	95
HpyCH4IV	ACGT	7 cut(s)	1375, 1735, 1774, 2983, 3929, 4015, 4044
HpySE526I	ACGT	7 cut(s)	1375, 1735, 1774, 2983, 3929, 4015, 4044
Hsp92I	GRCGYC	1 cut(s)	343
HspAI	GCGC	2 cut(s)	97, 343
KasI	GGCGCC	1 cut(s)	342
KpnI	GGTACC	1 cut(s)	1505
Ksp22I	TGATCA	2 cut(s)	685, 2502
KspI	CCGCGG	1 cut(s)	305
LguI	GCTCTTC	1 cut(s)	3429
LmnI	GCTCC	9 cut(s)	25, 104, 123, 289, 1219, 1467, 1594, 1744, 3098
MaeII	ACGT	7 cut(s)	1375, 1735, 1774, 2983, 3929, 4015, 4044
MfeI	CAATTG	2 cut(s)	1879, 2175
MflI	RGATCY	4 cut(s)	4, 2090, 3253, 3265
MhlI	GDGCHC	7 cut(s)	30, 707, 1599, 1741, 1816, 2989, 3851
Mly113I	GGCGCC	1 cut(s)	343
MlyI	GAGTC	5 cut(s)	173, 437, 932, 1121, 1711
MmeI	TCCRAC	6 cut(s)	126, 267, 888, 1252, 2434, 3192
Mph1103I	ATGCAT	3 cut(s)	1171, 2511, 3288
MroXI	GAANNNNTTC	1 cut(s)	3450
MslI	CAYNNNNRTG	3 cut(s)	3063, 3124, 3899
MspA1I	CMGCKG	5 cut(s)	60, 304, 1142, 2000, 3208
MspI	CCGG	8 cut(s)	37, 51, 161, 230, 453, 974, 1713, 2012
MunI	CAATTG	2 cut(s)	1879, 2175
Mva1269I	GAATGC	1 cut(s)	2266
MvaI	CCWGG	9 cut(s)	507, 663, 982, 2334, 2467, 2769, 2804, 2909, 3617
MvnI	CGCG	3 cut(s)	304, 1764, 2211
NarI	GGCGCC	1 cut(s)	343
NciI	CCSGG	3 cut(s)	38, 974, 2013
NcoI	CCATGG	2 cut(s)	3061, 3349
NdeI	CATATG	1 cut(s)	3125
NheI	GCTAGC	1 cut(s)	4034
NmuCI	GTSAC	5 cut(s)	968, 1917, 1944, 2063, 4016
NsiI	ATGCAT	3 cut(s)	1171, 2511, 3288
NspI	RCATGY	5 cut(s)	2011, 2447, 2548, 3286, 3552
OliI	CACNNNNGTG	1 cut(s)	3063
PaeI	GCATGC	1 cut(s)	2011
PaeR7I	CTCGAG	1 cut(s)	74
PagI	TCATGA	3 cut(s)	1024, 1750, 2784
PaqCI	CACCTGC	1 cut(s)	2275
PceI	AGGCCT	1 cut(s)	980
PciSI	GCTCTTC	1 cut(s)	3429
PcsI	WCGNNNNNNNCGW	2 cut(s)	99, 164
PctI	GAATGC	1 cut(s)	2266
PdmI	GAANNNNTTC	1 cut(s)	3450
PfeI	GAWTC	8 cut(s)	212, 521, 827, 1981, 2050, 2474, 2945, 3802
PflFI	GACNNNGTC	1 cut(s)	107
PflMI	CCANNNNNTGG	1 cut(s)	3062
PfoI	TCCNGGA	1 cut(s)	505
PleI	GAGTC	5 cut(s)	172, 436, 932, 1120, 1710
PluTI	GGCGCC	1 cut(s)	346
PmaCI	CACGTG	1 cut(s)	1736
PmlI	CACGTG	1 cut(s)	1736
PpsI	GAGTC	5 cut(s)	172, 436, 932, 1120, 1710
Ppu21I	YACGTR	3 cut(s)	1376, 1736, 4045
PshAI	GACNNNNGTC	1 cut(s)	642
PshBI	ATTAAT	1 cut(s)	3641
PsiI	TTATAA	1 cut(s)	1457
Psp124BI	GAGCTC	1 cut(s)	30
Psp6I	CCWGG	9 cut(s)	505, 661, 980, 2332, 2465, 2767, 2802, 2907, 3615
PspCI	CACGTG	1 cut(s)	1736
PspEI	GGTNACC	1 cut(s)	968
PspFI	CCCAGC	2 cut(s)	721, 3074
PspGI	CCWGG	9 cut(s)	505, 661, 980, 2332, 2465, 2767, 2802, 2907, 3615
PspPI	GGNCC	6 cut(s)	455, 596, 637, 644, 1302, 3906
PspXI	VCTCGAGB	1 cut(s)	74
PsuI	RGATCY	4 cut(s)	4, 2090, 3253, 3265
PsyI	GACNNNGTC	1 cut(s)	107
PvuII	CAGCTG	3 cut(s)	1142, 2000, 3208
RseI	CAYNNNNRTG	3 cut(s)	3063, 3124, 3899
SacI	GAGCTC	1 cut(s)	30
SacII	CCGCGG	1 cut(s)	305
SapI	GCTCTTC	1 cut(s)	3429
Sau96I	GGNCC	6 cut(s)	455, 596, 637, 644, 1302, 3906
SchI	GAGTC	5 cut(s)	173, 437, 932, 1121, 1711
SduI	GDGCHC	7 cut(s)	30, 707, 1599, 1741, 1816, 2989, 3851
SfcI	CTRYAG	1 cut(s)	3304
SfiI	GGCCNNNNNGGCC	1 cut(s)	60
SfoI	GGCGCC	1 cut(s)	344
Sfr274I	CTCGAG	1 cut(s)	74
Sfr303I	CCGCGG	1 cut(s)	305
SgrAI	CRCCGGYG	1 cut(s)	1712
SgrBI	CCGCGG	1 cut(s)	305
SinI	GGWCC	4 cut(s)	455, 637, 644, 3906
SlaI	CTCGAG	1 cut(s)	74
SmiMI	CAYNNNNRTG	3 cut(s)	3063, 3124, 3899
SmlI	CTYRAG	6 cut(s)	74, 650, 1100, 1297, 3164, 3806
SmoI	CTYRAG	6 cut(s)	74, 650, 1100, 1297, 3164, 3806
SnaBI	TACGTA	1 cut(s)	4045
SpeI	ACTAGT	1 cut(s)	962
SphI	GCATGC	1 cut(s)	2011
SseBI	AGGCCT	1 cut(s)	980
SspDI	GGCGCC	1 cut(s)	342
SspI	AATATT	2 cut(s)	1338, 2721
SstI	GAGCTC	1 cut(s)	30
StuI	AGGCCT	1 cut(s)	980
StyI	CCWWGG	4 cut(s)	21, 640, 3061, 3349
TaiI	ACGT	7 cut(s)	1378, 1738, 1777, 2986, 3932, 4018, 4047
TaqI	TCGA	7 cut(s)	75, 93, 167, 396, 1266, 1425, 2643
TatI	WGTACW	3 cut(s)	280, 745, 3723
TauI	GCSGC	6 cut(s)	63, 139, 304, 1725, 1728, 1760
TfiI	GAWTC	8 cut(s)	212, 521, 827, 1981, 2050, 2474, 2945, 3802
TscAI	CASTG	6 cut(s)	254, 1288, 1604, 2947, 3184, 3204
TseFI	GTSAC	5 cut(s)	968, 1917, 1944, 2063, 4016
Tsp45I	GTSAC	5 cut(s)	968, 1917, 1944, 2063, 4016
TspGWI	ACGGA	4 cut(s)	1346, 1547, 1552, 3493
TspRI	CASTG	6 cut(s)	254, 1288, 1604, 2947, 3184, 3204
Tth111I	GACNNNGTC	1 cut(s)	107
Van91I	CCANNNNNTGG	1 cut(s)	3062
VpaK11BI	GGWCC	4 cut(s)	455, 637, 644, 3906
VspI	ATTAAT	1 cut(s)	3641
XapI	RAATTY	9 cut(s)	1018, 1262, 1828, 2101, 2300, 2315, 3153, 3559, 3938
XbaI	TCTAGA	1 cut(s)	532
XceI	RCATGY	5 cut(s)	2011, 2447, 2548, 3286, 3552
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2144
XhoI	CTCGAG	1 cut(s)	74
XmiI	GTMKAC	2 cut(s)	678, 1208
XmnI	GAANNNNTTC	1 cut(s)	3450
Zsp2I	ATGCAT	3 cut(s)	1171, 2511, 3288

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.