Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0005110

Physical Location & Seq

Genomic Coordinates Forward (+)

15379445 .. 15392703

Gene Structure 13259 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00012520.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4074 bp

ATGGATCCGATACAAGCAGCCAAGGAGCTCTCATTCCGGGTCGGCTTCTCCGGCCACAGCGGCCACCTCCGCCTCGAGCCTCTCTGCACCGTCGAGCGCTCCTACCCCGTCAAGTCGCTCCCCGATTTCGTCCTGCCGCCTGCATTTGCTAGGGAAACGCCGGAGTCGATAAAAGAGTATATAGAGGAGACATATCTGTTGCCGAGATTGGATTCTGATGTCTTTTCACCGGAAAAGGTTGGGAGGCAGTGGGACTTTGACTGGTTTGACAAGGCTAATGTACACTTGGAGCCGTCGTTGCCACGGTCTGTTGTCGCTCCGACATGGGAGTTACCGTTTAGGCGCCAGAAGAATGGATCAGAAGGAGGGATTTGGGAACCCAAATCTGTGCAGGTTGATGAAGCAGAACTTACCATAGAAGCACAAGAGTCTGGTTCGTTGCCACGCATGGCCGGACCAGCAAAGGATTTTGTATGGGGAAGCATCAGCAACCGTCCTTTTCGTCCAGGAGGCTTGGATGATTCCCAATCTCTAGAAAGAACTCTTCCTGATGGTGCTTCTAATGGCGAATGGTTACACCAACTTCTTACTGGTGGCCCTGCACAGGCTGTTCCACCAAGTTTTAAGCAAGGATTGGACCTTGGTCCTCTCAAGGCATATCCTGGCTCATGGAATGTATACAATGATCAAGGTTCAGTCAAGAGCACATCAGATGAAAAGCTGGGTTTGCAGAGTGAGTTGTCTGTACAGTTTGATGACTTGTTCAAAAAGGCTTGGGATGAGGACGTTGTTGAGTTTGAAGGAGATGGTCAATCGTCAGGATCGGAATCTGTTAAGTCAGAGGATGAAGCAAATGTGGTTGATGTTGATATTACCAGCAATCCTCCTGAGCCTGAACTGTCTGTGTTGGATGAGATTTTGTCAGTTGAGGCAGGAGAGTCAAAGTCAAGATTTAATGGAACTGGTGGTGAACAGAACCCAGAGGCTTGGGCTATTAGTGGACGTACTGAATGGATTTCGGAGAATTTTCATGATCTTGTTCCTGATAAGGCACTTGATTTTCCCTTTGAATTGGATACATTCCAAAAGGAGGCAATTTATTATCTTGAGAAGGGGGAGTCTGTCTTTGTATCTGCTCACACATCAGCTGGAAAGACAGTAGTTGCAGAATATGCATTTGCTTTGGCATCAAAACATTGCACGAGAGCTGTGTATACTGCTCCTATCAAGACCATCAGCAACCAAAAATATAGGGACTTCTGTGGAAAATTCGATGTTGGACTTCTCACTGGTGATGTTAGCTTGAGGCCCGAGGCTTCTTGTCTCATTATGACCACGGAAATATTAAGGTCAATGCTTTATCGGGGTGCTGACATCATACGTGACATTGAATGGGTTATCTTTGATGAGGTTCATTATGTCAATGATGTCGAAAGAGGTGTTGTTTGGGAAGAAGTTATTATAATGCTCCCGAGACACATCAACATTGTCCTCCTTTCAGCTACGGTACCGAACAAAGTTGAGTTTGCTGACTGGATTGGACGGACAAAGCAAAAGCAAATCCGTGTTACTGGGACTATAAAAAGACCAGTCCCATTGGAGCACTGCCTATTTTACTCCGGAGAACTTTACAAGATATGTGAAAGTGAAAGCTTCATTCCTCAGGGCTTCAAAGCTGCAAAAGATACATTCAAGAAAAAGAATAGTCCTGCCACCAGCGGCGGCGGTGGTGGATCACGTGCTCCAGCTTCTGCTTCAGCTTCTCACGATGGGGCTCGAGGTCAAAAACGTGAAACTTCACATTCAGGAAAACAGAAGCAATTGGGTGCCCATAACTCAGGGAATTTATCCAGAACTGGTGGAGCAAATCAAAACAACGGCAATGGCATGAACAATTGGGGTTTAAGAAGATCAGATGCTTCTTCGTGGTTGTCACTTATTAACAAGCTCTCAAAGAAGTCACTATTACCTGTTGTTATATTTTGTTTCTCAAAGAATCGCTGTGATAGGTCAGCTGATAGCATGTCGGGAATTGACCTCACAAGTAGTTCTGAGAAAAGTCAGATTCGTGTTTTCTGTGACAAAGCATTTTCAAGGCTGAAGGGATCCGACAGGAATTTACCTCAGGTTGTCAGAGTTCAAAACCTTCTCCATAGAGGGATTGGTGTCCATCATGCAGGACTTCTTCCAATTGTTAAGGAAGTTGTTGAAATGCTCTTTTGTCGAGGTGTGATAAAGGTTCTGTTTTCAACAGAGACATTTGCAATGGGAGTGAATGCACCAGCTAGAACGGTTGTCTTTGATACATTAAGGAAATTTGATGGCAAGGAATTTAGACAACTACTACCAGGAGAATACACTCAAATGGCAGGCCGTGCTGGCAGGAGAGGACTTGATAAAATTGGTACAGTTATTGTAATGTGCCGTGACGAAATCCTAGAAGAGAGGGATTTGAAGCATGTCATGGTTGGAAGTGCAACCAGGCTAGAATCTCAGTTTCGGCTTACTTATATTATGATCATGCATCTTCTTCGTGTTGAAGAACTGAAGGTGGAGGACATGCTGAAAAGAAGTTTTGCTGAGTTCCATGCTCAGAAGAAACTACCAGATATGCAGCAACTTCTGATGCGAAAGCTTGCTCAGCCTACAAAAAGTATCGAATGTATAAAAGGGGAACCAGCTATTGAGGAGTATTATGACATGTACTCAGAAAAGGAGAAGCATAAAGCAGAAGTATTGGAGGCAGTAATGCAGTCCCCTGGTGCCCAACAATTTCTTACATCCGGAAGAGTGGTTGTCATGAAATCACAATCAGCCCAGAAAAACTTGCTTGGAGTTGTTGTAAAATCACCTTCTTCTAGTAATAAGCAACACATCGTTCTAGTGCTGAAACCTGAACTACCAGCGACAATCCAAACTCCCCTGGCTAGTGGTAACTTACAAGATACCAAAAACACTGATTCTTCTCAGGGTTACTACATGGTAGCGAAATCTAAACGTGGTTTTGAAGAAGAGTATTGTACCTCTGTTTCTTCACGCAAAGGATCAGGTGTCATCAACATAAAATTACCACACCATGGTGCTGCTGCTGGGGTGAGATACGAGGTTAGAGGAGCCGATATCACAGACTTCTCATTCATGTGCAGTAGCAAAATAAAGATTGACCTAGTCCGGCTTCTTGAGGACTGTAGCAGTGCTGCTTACTCCAAGACAGTGCAACTGCTGTTGGACACAAAACCTAAAGGAGAGGAGTACCCTCCAGCCTTAGATCCATTGAAAGATCTGAAGTTGAAAGACATGCATCTTGTGGAAACATACTACAAATGGACAAGCTTAGTGCAGAAGATGGCAAAGAATAAGTGCGATGGATGTATTAAATTGGAGGAACACATTACGTTAGCAAAAGAGATAAAGAGACACAAAGAGGAAGTTAATGCTCTTAAGTTTCAAATGTCAGATGAAGCACTTCAACAGATGCCAGATTTTCAAGGCCGGATAGATGTTCTGAAGGCAATCGGATGTATAGATGCTGACCTTGTAGTTCAAATAAAAGGCCGTGTCGCGTGTGAGATGAATTCTGGGGAGGAGTTGATCTGCACAGAGTGTTTGTTTGAGAACCAACTAGATGACCTGGAACCAGAAGAAGCGGTGGCATTAATGTCGTCCTTCGTTTTTCAGCAGAAGAATACTTCTGAACCTTCACTTACTCCTAAACTTTCTATGGCGAAAGAGAGATTGTACAATACAGCAATAAGACTAGGCGAGTGTCAAGCTCACTTCAAACTTCCAATAAATCCAGAAGAGTACGCCAAAGAGAATCTCAAGTTTGGTCTTGTTGAAGTTGTTTATGAGTGGGCAAAGGGCACACCATTTGCAGATATTTGTGAACTAACAGATGTTCCTGAAGGCATGATAGTGCGGACCATTGTTAGACTTGATGAGACGTGTCGTGAATTCAAAAATGCTGCATCTATTATGGGTAATTCTGCTCTATACAAGAAAATGGAGACGGCTTCAAATGCAATAAAACGTGACATTGTTTTCGCAGCTAGCTTGTATGTAACAGGAGTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000184 GO:0000288 GO:0000291 GO:0000956 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010035 GO:0010038 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0015672 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0019439 GO:0030001 GO:0032991 GO:0034427 GO:0034641 GO:0034655 GO:0035864 GO:0040029 GO:0042221 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0050789 GO:0050896 GO:0051179 GO:0051234 GO:0055087 GO:0060255 GO:0065007 GO:0070035 GO:0070478 GO:0071704 GO:0090304 GO:0140098 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

DEAD DSHCT Helicase_C rRNA_proc-arch

Protein Analysis

1357

Amino Acids

151.17

Weight (kDa)

5.96

Isoelectric Point (pI)

44.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ski2_N	PF17911	114 - 215	2.6e-09	Ski2 N-terminal region
DEAD	PF00270	361 - 508	5.2e-23	DEAD/DEAH box helicase
Helicase_C	PF00271	684 - 797	6e-06	Helicase conserved C-terminal domain
MTR4-like_stalk	PF21408	852 - 916	1.7e-12	Exosome RNA helicase MTR4-like, stalk
MTR4_beta-barrel	PF13234	920 - 1153	3.9e-23	Mtr4-like, beta-barrel domain
DSHCT	PF08148	1179 - 1349	9.7e-59	DSHCT (NUC185) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0005110)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46960 AT3G46960 AT3G46960
fragaria_vesca	FvH4_3g37880 FvH4_3g37880 FvH4_3g37880 FvH4_3g37880 FvH4_3g37880
malus_domestica	MD03G1077900.v1.1 MD11G1082500.v1.1
prunus_persica	Prupe.6G062900_v2.0.a1 Prupe.6G062900_v2.0.a1 Prupe.6G062900_v2.0.a1
pyrus_communis	pycom03g06210 pycom11g07060
rosa_chinensis	RchiOBHm_Chr5g0068091
rosa_laevigata	RLG00000035954
rosa_multiflora	Rmu_sc0000435.1_g000017
rosa_roxburghii	Rroxscaffold_1G00012520 Rroxscaffold_1G00013260
rosa_rugosa	Rorug05G0387900 Rorug05G0388300 Rorug05G0388400 Rorug05G0388500
rosa_samantha	Rh5AG446500 Rh5BG464600 Rh5CG486400 Rh5DG478400
rosa_wichuraiana	Rw5G041700 Rw5G041740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1463
Acc36I	ACCTGC	1 cut(s)	382
Acc65I	GGTACC	1 cut(s)	1507
AccB1I	GGYRCC	4 cut(s)	342, 1507, 1826, 2762
AccB7I	CCANNNNNTGG	1 cut(s)	3077
AccI	GTMKAC	2 cut(s)	678, 1214
AccII	CGCG	1 cut(s)	3563
AccIII	TCCGGA	2 cut(s)	1619, 2783
AciI	CCGC	8 cut(s)	60, 70, 137, 1719, 1722, 1725, 3647, 3919
AclWI	GGATC	8 cut(s)	12, 364, 829, 1741, 2100, 2113, 3052, 3263
AcoI	YGGCCR	3 cut(s)	52, 61, 450
AcsI	RAATTY	8 cut(s)	1024, 1268, 1843, 2116, 2315, 2330, 3574, 3953
AcuI	CTGAAG	6 cut(s)	1740, 2120, 2567, 3305, 3527, 3924
AcvI	CACGTG	1 cut(s)	1739
AcyI	GRCGYC	1 cut(s)	343
AdeI	CACNNNGTG	1 cut(s)	3603
AfeI	AGCGCT	1 cut(s)	98
AfiI	CCNNNNNNNGG	3 cut(s)	604, 1090, 3077
AflII	CTTAAG	1 cut(s)	3440
AflIII	ACRYGT	2 cut(s)	2700, 3944
AjiI	CACGTC	1 cut(s)	3945
AjnI	CCWGG	7 cut(s)	505, 661, 2347, 2480, 2758, 2922, 3630
AleI	CACNNNNGTG	1 cut(s)	3078
Alw21I	GWGCWC	4 cut(s)	30, 707, 1605, 1744
Alw26I	GTCTC	7 cut(s)	182, 1328, 1468, 2249, 3409, 3935, 4001
AlwI	GGATC	8 cut(s)	12, 364, 829, 1741, 2100, 2113, 3052, 3263
AlwNI	CAGNNNCTG	1 cut(s)	1751
Ama87I	CYCGRG	4 cut(s)	74, 1310, 1471, 1776
Aor13HI	TCCGGA	2 cut(s)	1619, 2783
Aor51HI	AGCGCT	1 cut(s)	98
AoxI	GGCC	8 cut(s)	52, 61, 450, 595, 1307, 2371, 3490, 3553
ApeKI	GCWGC	8 cut(s)	17, 1676, 2614, 3083, 3086, 3197, 3965, 4046
ApoI	RAATTY	8 cut(s)	1024, 1268, 1843, 2116, 2315, 2330, 3574, 3953
ArsI	GACNNNNNNTTYG	2 cut(s)	4025, 4057
AseI	ATTAAT	1 cut(s)	3656
Asp700I	GAANNNNTTC	1 cut(s)	3465
Asp718I	GGTACC	1 cut(s)	1507
AspLEI	GCGC	2 cut(s)	99, 345
AspS9I	GGNCC	6 cut(s)	455, 596, 637, 644, 1308, 3921
AsuC2I	CCSGG	1 cut(s)	38
AsuHPI	GGTGA	5 cut(s)	219, 980, 1304, 2841, 3106
AsuNHI	GCTAGC	1 cut(s)	4049
AvaI	CYCGRG	4 cut(s)	74, 1310, 1471, 1776
AvaII	GGWCC	4 cut(s)	455, 637, 644, 3921
AxyI	CCTNAGG	2 cut(s)	1662, 2124
BaeGI	GKGCMC	3 cut(s)	1831, 2767, 3866
BamHI	GGATCC	2 cut(s)	4, 2105
BanI	GGYRCC	4 cut(s)	342, 1507, 1826, 2762
BanII	GRGCYC	2 cut(s)	30, 1777
BauI	CACGAG	1 cut(s)	1201
BbrPI	CACGTG	1 cut(s)	1739
Bbv12I	GWGCWC	4 cut(s)	30, 707, 1605, 1744
BbvI	GCAGC	8 cut(s)	29, 1663, 2626, 3070, 3073, 3184, 3952, 4058
BccI	CCATC	8 cut(s)	545, 800, 1241, 1763, 2178, 2315, 3340, 3359
BceAI	ACGGC	6 cut(s)	277, 1894, 2358, 2409, 3540, 4026
BcgI	CGANNNNNNTGC	4 cut(s)	2513, 2547, 3642, 3676
BciT130I	CCWGG	7 cut(s)	507, 663, 2349, 2482, 2760, 2924, 3632
BciVI	GTATCC	1 cut(s)	1069
BclI	TGATCA	2 cut(s)	685, 2517
BcnI	CCSGG	1 cut(s)	38
BcoDI	GTCTC	7 cut(s)	182, 1328, 1468, 2249, 3409, 3935, 4001
BfmI	CTRYAG	1 cut(s)	3187
BfoI	RGCGCY	2 cut(s)	100, 346
BfrI	CTTAAG	1 cut(s)	3440
BfuAI	ACCTGC	1 cut(s)	382
BfuI	GTATCC	1 cut(s)	1069
BglI	GCCNNNNNGGC	2 cut(s)	60, 2379
BglII	AGATCT	1 cut(s)	3280
BlpI	GCTNAGC	1 cut(s)	2640
Bme1390I	CCNGG	8 cut(s)	38, 507, 663, 2349, 2482, 2760, 2924, 3632
Bme18I	GGWCC	4 cut(s)	455, 637, 644, 3921
BmeT110I	CYCGRG	4 cut(s)	74, 1310, 1471, 1776
BmgBI	CACGTC	1 cut(s)	3945
BmgT120I	GGNCC	6 cut(s)	455, 596, 637, 644, 1308, 3921
BmrFI	CCNGG	8 cut(s)	38, 507, 663, 2349, 2482, 2760, 2924, 3632
BmrI	ACTGGG	1 cut(s)	1581
BmsI	GCATC	9 cut(s)	492, 1196, 1906, 2533, 2616, 3310, 3465, 3517, 3977
BmtI	GCTAGC	1 cut(s)	4053
BmuI	ACTGGG	1 cut(s)	1581
BoxI	GACNNNNGTC	1 cut(s)	642
BpmI	CTGGAG	2 cut(s)	1728, 3243
Bpu10I	CCTNAGC	1 cut(s)	888
Bpu1102I	GCTNAGC	1 cut(s)	2640
BpuEI	CTTGAG	5 cut(s)	635, 1127, 1324, 3200, 3806
BpuMI	CCSGG	1 cut(s)	38
BsaAI	YACGTR	2 cut(s)	1382, 1739
BsaBI	GATNNNNATC	1 cut(s)	826
BsaHI	GRCGYC	1 cut(s)	343
BsaJI	CCNNGG	8 cut(s)	21, 302, 640, 1311, 1335, 2758, 2922, 3076
BsaWI	WCCGGW	3 cut(s)	229, 1619, 2783
BsaXI	ACNNNNNCTCC	6 cut(s)	281, 311, 320, 350, 2826, 2856
Bsc4I	CCNNNNNNNGG	3 cut(s)	604, 1090, 3077
Bse1I	ACTGG	8 cut(s)	266, 595, 967, 1294, 1538, 1576, 1589, 1861
Bse21I	CCTNAGG	2 cut(s)	1662, 2124
Bse3DI	GCAATG	3 cut(s)	1195, 1888, 2271
Bse8I	GATNNNNATC	1 cut(s)	826
BseAI	TCCGGA	2 cut(s)	1619, 2783
BseBI	CCWGG	7 cut(s)	507, 663, 2349, 2482, 2760, 2924, 3632
BseDI	CCNNGG	8 cut(s)	21, 302, 640, 1311, 1335, 2758, 2922, 3076
BseGI	GGATG	7 cut(s)	523, 784, 850, 916, 2780, 3374, 3524
BseJI	GATNNNNATC	1 cut(s)	826
BseLI	CCNNNNNNNGG	3 cut(s)	604, 1090, 3077
BseMI	GCAATG	3 cut(s)	1195, 1888, 2271
BseNI	ACTGG	8 cut(s)	266, 595, 967, 1294, 1538, 1576, 1589, 1861
BseRI	GAGGAG	5 cut(s)	200, 2702, 3126, 3263, 3599
BseSI	GKGCMC	3 cut(s)	1831, 2767, 3866
BseXI	GCAGC	8 cut(s)	29, 1663, 2626, 3070, 3073, 3184, 3952, 4058
BseYI	CCCAGC	2 cut(s)	721, 3089
BsgI	GTGCAG	6 cut(s)	70, 410, 585, 3163, 3359, 3580
Bsh1236I	CGCG	1 cut(s)	3563
BshFI	GGCC	8 cut(s)	54, 63, 452, 597, 1309, 2373, 3492, 3555
BshNI	GGYRCC	4 cut(s)	342, 1507, 1826, 2762
BsiHKAI	GWGCWC	4 cut(s)	30, 707, 1605, 1744
BsiHKCI	CYCGRG	4 cut(s)	74, 1310, 1471, 1776
BsiSI	CCGG	9 cut(s)	37, 51, 161, 230, 453, 1620, 2784, 3172, 3493
BslFI	GGGAC	5 cut(s)	266, 1268, 1577, 1588, 2740
BslI	CCNNNNNNNGG	3 cut(s)	604, 1090, 3077
BsmAI	GTCTC	7 cut(s)	182, 1328, 1468, 2249, 3409, 3935, 4001
BsmBI	CGTCTC	2 cut(s)	3935, 4001
BsmFI	GGGAC	5 cut(s)	266, 1268, 1577, 1588, 2740
BsmI	GAATGC	1 cut(s)	2281
BsnI	GGCC	8 cut(s)	54, 63, 452, 597, 1309, 2373, 3492, 3555
BsoBI	CYCGRG	4 cut(s)	74, 1310, 1471, 1776
Bsp1286I	GDGCHC	8 cut(s)	30, 707, 1605, 1744, 1777, 1831, 2767, 3866
Bsp13I	TCCGGA	2 cut(s)	1619, 2783
Bsp1407I	TGTACA	3 cut(s)	280, 745, 3738
Bsp1720I	GCTNAGC	1 cut(s)	2640
Bsp19I	CCATGG	1 cut(s)	3076
BspACI	CCGC	8 cut(s)	60, 70, 137, 1719, 1722, 1725, 3647, 3919
BspANI	GGCC	8 cut(s)	54, 63, 452, 597, 1309, 2373, 3492, 3555
BspEI	TCCGGA	2 cut(s)	1619, 2783
BspFNI	CGCG	1 cut(s)	3563
BspHI	TCATGA	2 cut(s)	1030, 2799
BspMI	ACCTGC	1 cut(s)	382
BspOI	GCTAGC	1 cut(s)	4053
BspPI	GGATC	8 cut(s)	12, 364, 829, 1741, 2100, 2113, 3052, 3263
BspT107I	GGYRCC	4 cut(s)	342, 1507, 1826, 2762
BspTI	CTTAAG	1 cut(s)	3440
BsrDI	GCAATG	3 cut(s)	1195, 1888, 2271
BsrGI	TGTACA	3 cut(s)	280, 745, 3738
BsrI	ACTGG	8 cut(s)	266, 595, 967, 1294, 1538, 1576, 1589, 1861
BssECI	CCNNGG	8 cut(s)	21, 302, 640, 1311, 1335, 2758, 2922, 3076
BssNAI	GTATAC	2 cut(s)	679, 1215
BssNI	GRCGYC	1 cut(s)	343
BssSI	CACGAG	1 cut(s)	1201
BssT1I	CCWWGG	3 cut(s)	21, 640, 3076
Bst1107I	GTATAC	2 cut(s)	679, 1215
Bst2BI	CACGAG	1 cut(s)	1201
Bst2UI	CCWGG	7 cut(s)	507, 663, 2349, 2482, 2760, 2924, 3632
Bst6I	CTCTTC	5 cut(s)	549, 2436, 2782, 3006, 3795
BstACI	GRCGYC	1 cut(s)	343
BstAFI	CTTAAG	1 cut(s)	3440
BstAPI	GCANNNNNTGC	2 cut(s)	1172, 2375
BstAUI	TGTACA	3 cut(s)	280, 745, 3738
BstBAI	YACGTR	2 cut(s)	1382, 1739
BstC8I	GCNNGC	5 cut(s)	141, 2371, 2380, 2637, 4051
BstDSI	CCRYGG	3 cut(s)	302, 1335, 3076
BstF5I	GGATG	7 cut(s)	523, 784, 850, 916, 2780, 3374, 3524
BstFNI	CGCG	1 cut(s)	3563
BstH2I	RGCGCY	2 cut(s)	100, 346
BstHHI	GCGC	2 cut(s)	99, 345
BstMAI	GTCTC	7 cut(s)	182, 1328, 1468, 2249, 3409, 3935, 4001
BstNI	CCWGG	7 cut(s)	507, 663, 2349, 2482, 2760, 2924, 3632
BstNSI	RCATGY	5 cut(s)	2026, 2462, 2563, 2704, 3301
BstPAI	GACNNNNGTC	1 cut(s)	642
BstSCI	CCNGG	8 cut(s)	36, 505, 661, 2347, 2480, 2758, 2922, 3630
BstSFI	CTRYAG	1 cut(s)	3187
BstSLI	GKGCMC	3 cut(s)	1831, 2767, 3866
BstUI	CGCG	1 cut(s)	3563
BstV1I	GCAGC	8 cut(s)	29, 1663, 2626, 3070, 3073, 3184, 3952, 4058
BstX2I	RGATCY	4 cut(s)	4, 2105, 3268, 3280
BstXI	CCANNNNNNTGG	2 cut(s)	353, 987
BstYI	RGATCY	4 cut(s)	4, 2105, 3268, 3280
BstZ17I	GTATAC	2 cut(s)	679, 1215
Bsu36I	CCTNAGG	2 cut(s)	1662, 2124
BsuI	GTATCC	1 cut(s)	1069
BsuRI	GGCC	8 cut(s)	54, 63, 452, 597, 1309, 2373, 3492, 3555
BtgI	CCRYGG	3 cut(s)	302, 1335, 3076
BtgZI	GCGATG	1 cut(s)	3378
BtrI	CACGTC	1 cut(s)	3945
BtsCI	GGATG	7 cut(s)	523, 784, 850, 916, 2780, 3374, 3524
BtsI	GCAGTG	3 cut(s)	254, 1603, 3199
BtsIMutI	CAGTG	6 cut(s)	254, 1287, 1603, 2955, 3199, 3219
BveI	ACCTGC	1 cut(s)	382
Cac8I	GCNNGC	5 cut(s)	141, 2371, 2380, 2637, 4051
CaiI	CAGNNNCTG	1 cut(s)	1751
CciI	TCATGA	2 cut(s)	1030, 2799
CfoI	GCGC	2 cut(s)	99, 345
Cfr13I	GGNCC	6 cut(s)	455, 596, 637, 644, 1308, 3921
DinI	GGCGCC	1 cut(s)	344
DraIII	CACNNNGTG	1 cut(s)	3603
EaeI	YGGCCR	3 cut(s)	52, 61, 450
Eam1104I	CTCTTC	5 cut(s)	549, 2436, 2782, 3006, 3795
EarI	CTCTTC	5 cut(s)	549, 2436, 2782, 3006, 3795
EciI	GGCGGA	1 cut(s)	59
Ecl136II	GAGCTC	1 cut(s)	28
Eco130I	CCWWGG	3 cut(s)	21, 640, 3076
Eco24I	GRGCYC	2 cut(s)	30, 1777
Eco32I	GATATC	1 cut(s)	3121
Eco47I	GGWCC	4 cut(s)	455, 637, 644, 3921
Eco47III	AGCGCT	1 cut(s)	98
Eco53kI	GAGCTC	1 cut(s)	28
Eco57I	CTGAAG	6 cut(s)	1740, 2120, 2567, 3305, 3527, 3924
Eco72I	CACGTG	1 cut(s)	1739
Eco81I	CCTNAGG	2 cut(s)	1662, 2124
Eco88I	CYCGRG	4 cut(s)	74, 1310, 1471, 1776
EcoICRI	GAGCTC	1 cut(s)	28
EcoRI	GAATTC	2 cut(s)	3574, 3953
EcoRII	CCWGG	7 cut(s)	505, 661, 2347, 2480, 2758, 2922, 3630
EcoRV	GATATC	1 cut(s)	3121
EcoT14I	CCWWGG	3 cut(s)	21, 640, 3076
EcoT22I	ATGCAT	3 cut(s)	1177, 2526, 3303
EcoT38I	GRGCYC	2 cut(s)	30, 1777
EgeI	GGCGCC	1 cut(s)	344
EheI	GGCGCC	1 cut(s)	344
ErhI	CCWWGG	3 cut(s)	21, 640, 3076
Esp3I	CGTCTC	2 cut(s)	3935, 4001
FalI	AAGNNNNNCTT	2 cut(s)	393, 425
FaqI	GGGAC	5 cut(s)	266, 1268, 1577, 1588, 2740
FbaI	TGATCA	2 cut(s)	685, 2517
FblI	GTMKAC	2 cut(s)	678, 1214
FokI	GGATG	7 cut(s)	530, 791, 857, 923, 2767, 3381, 3531
FriOI	GRGCYC	2 cut(s)	30, 1777
GlaI	GCGC	2 cut(s)	98, 344
GsaI	CCCAGC	2 cut(s)	725, 3093
GsuI	CTGGAG	2 cut(s)	1728, 3243
HaeII	RGCGCY	2 cut(s)	100, 346
HaeIII	GGCC	8 cut(s)	54, 63, 452, 597, 1309, 2373, 3492, 3555
HapII	CCGG	9 cut(s)	37, 51, 161, 230, 453, 1620, 2784, 3172, 3493
HhaI	GCGC	2 cut(s)	99, 345
Hin1I	GRCGYC	1 cut(s)	343
Hin6I	GCGC	2 cut(s)	97, 343
HinP1I	GCGC	2 cut(s)	97, 343
HindIII	AAGCTT	3 cut(s)	1651, 2633, 3331
HpaII	CCGG	9 cut(s)	37, 51, 161, 230, 453, 1620, 2784, 3172, 3493
HphI	GGTGA	5 cut(s)	219, 980, 1304, 2841, 3106
Hpy166II	GTNNAC	6 cut(s)	283, 679, 971, 1001, 1215, 3887
Hpy8I	GTNNAC	6 cut(s)	283, 679, 971, 1001, 1215, 3887
Hpy99I	CGWCG	2 cut(s)	95, 298
HpyCH4IV	ACGT	9 cut(s)	786, 1003, 1381, 1738, 1789, 2998, 3395, 3944, 4030
HpySE526I	ACGT	9 cut(s)	786, 1003, 1381, 1738, 1789, 2998, 3395, 3944, 4030
Hsp92I	GRCGYC	1 cut(s)	343
HspAI	GCGC	2 cut(s)	97, 343
KasI	GGCGCC	1 cut(s)	342
Kpn2I	TCCGGA	2 cut(s)	1619, 2783
KpnI	GGTACC	1 cut(s)	1511
Ksp22I	TGATCA	2 cut(s)	685, 2517
Lsp1109I	GCAGC	8 cut(s)	29, 1663, 2626, 3070, 3073, 3184, 3952, 4058
LweI	GCATC	9 cut(s)	492, 1196, 1906, 2533, 2616, 3310, 3465, 3517, 3977
MaeII	ACGT	9 cut(s)	786, 1003, 1381, 1738, 1789, 2998, 3395, 3944, 4030
MfeI	CAATTG	3 cut(s)	1820, 1894, 2190
MflI	RGATCY	4 cut(s)	4, 2105, 3268, 3280
MhlI	GDGCHC	8 cut(s)	30, 707, 1605, 1744, 1777, 1831, 2767, 3866
Mly113I	GGCGCC	1 cut(s)	343
MlyI	GAGTC	4 cut(s)	173, 437, 947, 1127
MmeI	TCCRAC	6 cut(s)	344, 888, 1258, 2133, 2449, 3207
Mph1103I	ATGCAT	3 cut(s)	1177, 2526, 3303
MroI	TCCGGA	2 cut(s)	1619, 2783
MroXI	GAANNNNTTC	1 cut(s)	3465
MslI	CAYNNNNRTG	4 cut(s)	2363, 3078, 3139, 3914
MspA1I	CMGCKG	4 cut(s)	60, 1148, 1719, 2015
MspCI	CTTAAG	1 cut(s)	3440
MspI	CCGG	9 cut(s)	37, 51, 161, 230, 453, 1620, 2784, 3172, 3493
MspR9I	CCNGG	8 cut(s)	38, 507, 663, 2349, 2482, 2760, 2924, 3632
MunI	CAATTG	3 cut(s)	1820, 1894, 2190
Mva1269I	GAATGC	1 cut(s)	2281
MvaI	CCWGG	7 cut(s)	507, 663, 2349, 2482, 2760, 2924, 3632
MvnI	CGCG	1 cut(s)	3563
NarI	GGCGCC	1 cut(s)	343
NciI	CCSGG	1 cut(s)	38
NcoI	CCATGG	1 cut(s)	3076
NheI	GCTAGC	1 cut(s)	4049
NmeAIII	GCCGAG	1 cut(s)	228
NmuCI	GTSAC	6 cut(s)	1382, 1932, 1959, 2078, 2426, 4031
NsiI	ATGCAT	3 cut(s)	1177, 2526, 3303
NspI	RCATGY	5 cut(s)	2026, 2462, 2563, 2704, 3301
OliI	CACNNNNGTG	1 cut(s)	3078
PaeR7I	CTCGAG	2 cut(s)	74, 1776
PagI	TCATGA	2 cut(s)	1030, 2799
PciI	ACATGT	1 cut(s)	2700
PcsI	WCGNNNNNNNCGW	1 cut(s)	164
PctI	GAATGC	1 cut(s)	2281
PdmI	GAANNNNTTC	1 cut(s)	3465
PfeI	GAWTC	8 cut(s)	212, 521, 827, 1996, 2065, 2489, 2960, 3817
PflFI	GACNNNGTC	1 cut(s)	3167
PflMI	CCANNNNNTGG	1 cut(s)	3077
PfoI	TCCNGGA	1 cut(s)	505
PleI	GAGTC	4 cut(s)	172, 436, 946, 1126
PluTI	GGCGCC	1 cut(s)	346
PmaCI	CACGTG	1 cut(s)	1739
PmlI	CACGTG	1 cut(s)	1739
PpsI	GAGTC	4 cut(s)	172, 436, 946, 1126
Ppu21I	YACGTR	2 cut(s)	1382, 1739
PscI	ACATGT	1 cut(s)	2700
PshAI	GACNNNNGTC	1 cut(s)	642
PshBI	ATTAAT	1 cut(s)	3656
PsiI	TTATAA	1 cut(s)	1463
Psp124BI	GAGCTC	1 cut(s)	30
Psp6I	CCWGG	7 cut(s)	505, 661, 2347, 2480, 2758, 2922, 3630
PspCI	CACGTG	1 cut(s)	1739
PspFI	CCCAGC	2 cut(s)	721, 3089
PspGI	CCWGG	7 cut(s)	505, 661, 2347, 2480, 2758, 2922, 3630
PspPI	GGNCC	6 cut(s)	455, 596, 637, 644, 1308, 3921
PspXI	VCTCGAGB	2 cut(s)	74, 1776
PstNI	CAGNNNCTG	1 cut(s)	1751
PsuI	RGATCY	4 cut(s)	4, 2105, 3268, 3280
PsyI	GACNNNGTC	1 cut(s)	3167
PvuII	CAGCTG	2 cut(s)	1148, 2015
RseI	CAYNNNNRTG	4 cut(s)	2363, 3078, 3139, 3914
SacI	GAGCTC	1 cut(s)	30
Sau96I	GGNCC	6 cut(s)	455, 596, 637, 644, 1308, 3921
SchI	GAGTC	4 cut(s)	173, 437, 947, 1127
ScrFI	CCNGG	8 cut(s)	38, 507, 663, 2349, 2482, 2760, 2924, 3632
SduI	GDGCHC	8 cut(s)	30, 707, 1605, 1744, 1777, 1831, 2767, 3866
SfaNI	GCATC	9 cut(s)	492, 1196, 1906, 2533, 2616, 3310, 3465, 3517, 3977
SfcI	CTRYAG	1 cut(s)	3187
SfiI	GGCCNNNNNGGCC	1 cut(s)	60
SfoI	GGCGCC	1 cut(s)	344
Sfr274I	CTCGAG	2 cut(s)	74, 1776
SinI	GGWCC	4 cut(s)	455, 637, 644, 3921
SlaI	CTCGAG	2 cut(s)	74, 1776
SmiMI	CAYNNNNRTG	4 cut(s)	2363, 3078, 3139, 3914
SmlI	CTYRAG	8 cut(s)	74, 650, 1106, 1303, 1776, 3179, 3440, 3821
SmoI	CTYRAG	8 cut(s)	74, 650, 1106, 1303, 1776, 3179, 3440, 3821
SsiI	CCGC	8 cut(s)	60, 70, 137, 1719, 1722, 1725, 3647, 3919
SspDI	GGCGCC	1 cut(s)	342
SspI	AATATT	1 cut(s)	1344
SstI	GAGCTC	1 cut(s)	30
StyD4I	CCNGG	8 cut(s)	36, 505, 661, 2347, 2480, 2758, 2922, 3630
StyI	CCWWGG	3 cut(s)	21, 640, 3076
TaiI	ACGT	9 cut(s)	789, 1006, 1384, 1741, 1792, 3001, 3398, 3947, 4033
TaqI	TCGA	8 cut(s)	75, 93, 167, 1272, 1431, 1777, 2224, 2658
TatI	WGTACW	4 cut(s)	280, 745, 2703, 3738
TauI	GCSGC	4 cut(s)	63, 139, 1722, 1725
TfiI	GAWTC	8 cut(s)	212, 521, 827, 1996, 2065, 2489, 2960, 3817
TscAI	CASTG	6 cut(s)	254, 1294, 1610, 2962, 3199, 3219
TseFI	GTSAC	6 cut(s)	1382, 1932, 1959, 2078, 2426, 4031
TseI	GCWGC	8 cut(s)	17, 1676, 2614, 3083, 3086, 3197, 3965, 4046
Tsp45I	GTSAC	6 cut(s)	1382, 1932, 1959, 2078, 2426, 4031
TspGWI	ACGGA	3 cut(s)	1352, 1553, 1558
TspRI	CASTG	6 cut(s)	254, 1294, 1610, 2962, 3199, 3219
Tth111I	GACNNNGTC	1 cut(s)	3167
Van91I	CCANNNNNTGG	1 cut(s)	3077
Vha464I	CTTAAG	1 cut(s)	3440
VpaK11BI	GGWCC	4 cut(s)	455, 637, 644, 3921
VspI	ATTAAT	1 cut(s)	3656
XapI	RAATTY	8 cut(s)	1024, 1268, 1843, 2116, 2315, 2330, 3574, 3953
XbaI	TCTAGA	1 cut(s)	532
XceI	RCATGY	5 cut(s)	2026, 2462, 2563, 2704, 3301
XcmI	CCANNNNNNNNNTGG	2 cut(s)	587, 2159
XhoI	CTCGAG	2 cut(s)	74, 1776
XmiI	GTMKAC	2 cut(s)	678, 1214
XmnI	GAANNNNTTC	1 cut(s)	3465
Zsp2I	ATGCAT	3 cut(s)	1177, 2526, 3303

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.