Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0005110

Physical Location & Seq

Genomic Coordinates Forward (+)

7014042 .. 7025897

Gene Structure 11856 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1082500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4056 bp

ATGGATCCGATAGAAGCAGCGAACGGGCTCTCGTTCCGGGTCGGATTCTCCGGCCACAGTGGCCACCTCCGGCTTGAGCCCCTCTCCACGGTTGAAAGCTCCGACCCCGTCAAGTCTCTCCCTGATTTCATTCTGCCGCCTGCTTTTCCTAGGGAGACGCCTGGATCAATAAAAGAGTATATAGAGGAGAAGTATCTATCGCTGAGATTGGATGACGAAGTATTTGCACCCGAAAAGGTTGGGAGGCAGTGGGATTTCGACTGGTTTGATAAGGCAGATGTTCCCTTGGAGCTATCACTGCCGCGCTGCGTTGTGGTTCCCACGTGGGAATTGCCATTTCGGCGCCAGAAGAATGGGTCGGCTGAAGGTCAATGGGAACCTAAATCTGTGCAGGTGGATGTGTCAGAACTTATAGTAGGAGCTCGAGAGTCTGGTTCATTGCCACGTTTGGCTGGACCAGCTAAGGATTTTGTACGGGGAAGCATCAACAACCGCCCTTTTCGTCCAGGAGGCTTGGATGATTCCCAATCTCTAGAAAGAGTTGTTCCTGATGGTGCTTGTAATGGCGAATGGGTACGCGAACTTCTGGTTGGTGGCTCTCCTCAGGCTGTACCTCCAAGCTTTAAGCAAGGATTGGACCTTGGTGATCTGAAGGCCTATCCATGCTCATGGAATGTATATAAGGATCAAAGTTCCATGGAAAGCACGTCAGATGAAAAACTGAGTGGGTTGTCTGTGCAGTTTGATGACCTGTTCGAGAAGGCTTGGGAAGAGGAAGTTGTTGAATTTGAAGGAGATGGTCAATTGTCAGGATCAGAATCTGTTAAGTCAGAGGATGAAGTAAAGGAGGTTGATGTTGCCATCGATTCTTGTGAGCCTGAATTGTCTTTGTTGGATGAGCTTTTGTCAGTTGAGGCAGGAGAAACAAAGTCAAGATCTAATGGAACTGGTGAAGAGGATGAACAAAATCCGAAGGTGGCTTGGGCTATTAGTGGAGGTACTGAATATATTGCAGAGAACTTTCATGATCTTGTTCCCAACAAAGCAATGGATTTTCCTTTTGAATTGGACAAATTCCAGAAGGAGGCTATTTATTATCTTGAAAAGGGGGACTCGGTATTTGTGGCTGCTCACACATCAGCTGGAAAGACAGTTGTTGCAGAATATGCATTTGCTTTAGCATCAAAACATTGCACCAGAGCTGTGTATACAGCTCCTATCAAAACCATCAGCAACCAAAAATATAGAGATTTGTGTGGAAAATTTGATGTTGGACTTCTCACTGGTGATGTTAGCTTGAGGCCAGAGGCTTCTTGTCTCATTATGACGACTGAAATATTGAGGTCAATGCTTTATCGCGGTGCTGACATAATACGTGATATTGAATGGGTTATCTTTGACGAAGTGCACTATGTCAATGATGTCGAAAGAGGTGTTGTTTGGGAAGAAGTTATTATAATGCTTCCAAGACACATCAATATTGTCCTCCTTTCAGCTACGGTACCAAACAAAGTTGAGTTTGCTGACTGGATTGGACGGACAAAGCAAAAGCAAATCCGTGTTACCGGGACTACAAAAAGACCAGTCCCATTGGAGCACTGTCTATTTTACTCCGGAGAACTATACAAAATATGTGAAAGTGAAAGCTTTATTCCCGAGGGGTTCAAAGCTGCAAAAGATGCATTTAAGAAAAAGAATATGAATTCTGCCACTGGTGGTAGTGGATCACAGGCACCAGCTTCAGCTTCTCACGATGGGGCTCGATCTCAAAAACAAAGTTCAAACTGGGGCGGAAAAGAGAAGAAGCAATCTGGTCCCCAAAATTCAGGGAATTTCTCCCGAACGAGAGGAGAAAGTCAAAACAATGGAAATGGCATGAACAGTTGGGGTTTAAGAAGATCAGATGCTTCTTTGTGGTTGTCACTTATTAACAAGCTCTCAAAGATGTCGCTATTACCTGTGGTGGTATTTTGTTTCTCAAAGAATCGCTGTGATAAGTCAGCTGATAGTATGTATGGGACTAACCTTACAAGTAGTTCTGAGAAAAGTGAGATTCGTGTTTTCTGTGACAAAGCATTTTCGCGGCTAAAGGGATCTGATAGGACTTTACCACAGGTTGTCAGAGTTCAAAGCCTTCTTCATAGAGGGATTGGTGTCCATCATGCTGGACTGCTTCCAATTGTTAAGGAAGTTGTGGAAATGCTTTTTTGTCGTGGTGTGATCAAGGTTTTGTTCTCAACAGAGACATTTGCAATGGGGGTTAATGCACCAGCTAGAACGGTTGTTTTTGATATATTAAGGAAATTTGATGGCAAGGAATTTAGACAATTACTTCCAGGAGAATACACTCAAATGGCAGGCCGTGCGGGCAGAAGAGGACTTGATAAAATTGGCACAGTTATTGTAATGTGCCGTGATGAAATCCTAGGAGAGAGTGATTTGAAGCATGTCATAGTTGGAAGTGCAACCCGGCTTGAATCTCAGTTTCGACTTACCTATATTATGATCCTGCATCTCCTTCGTGTTGAGGAACTGAAGGTGGAGGACATGCTGAAAAGAAGTTTTGCTGAGTTCCATGCTCAGAAGAAACTACCAGAACAGCAGCAACTTCTGATGCGAAAGCTTGCTCAGGCTACAAAACCTATCGAATGTATAAAAGGTGAACCAGCTATTGAGGAGTATTATGACATCCGCTCGGAAGCTGAGAATCATAACACGGAAATATTGGAGGCAGTTATGCAGTCTCCTGTTGCCCAACAATTTCTTACAACTGGGAGAGTGGTAGTCATGAAATCAGAGTCAGCGCAGGACCACTTGCTTGGAGTCGTCGTGAAAGCACCTAGTGCTACTAATAAACAATATATTGTTTTGGTGCTGAAACCTGAACTACCGCCGCAGACTCCACTGGGTAGTGGTAACTTGCAAGACAGTAAAAGCACGGATTATCCACAAGGTTTTTTTGTGGCACCAAAATCTAAACGTGGTCTTGATGAAGAGTATTGTTCTGGTGTCAAATCTCGGAAAGGAAAGGGTGTCATCAACATAAAATTACCACACCAGGGTTCTGCTGCTGGGGTGAGCTTTGAGGTTAAAGGAGTTGACAGCAAAGATTTTCTATGCATATGCAATTGCAAGATAAAAATTGACCAAGTTGGGCTTCTTGAAGAGAATAGCAGTGCTGCTTACTCCAAGACAGTTCAACAGCTGTTGGACACAAAATCCGACGAAAATAAGTACCCTCCAGCCCTGGATCCACATAAAGATCTGAAGTTGAGAGATGTGAATCTCGTGGCAACATACTACAAGTGGACTAACTTATTGCAAAAGATGGCAAAGAATAAGTGCCATGGATGTATAAAGTTGGACGAGCACATTAAGTTAGCAAGAGAGATGAAGAGACACAGTGAGGAAGTCGATGCTCTTAAGTTTCAAATGTCAGATGAAGCACTTAAACAAATGCCAGACTTTCAAGGCCGGATAGATGTTCTGCAGGAAATTGGATGTATCAATGCTGATCTTGTGGTTCAAATAAAAGGCCGTGTTGCTTGCGAAATGAACTCTGGGGAGGAGTTGATAGCCACAGAGTGTTTGTTTGAAAACCAACTGGATGACCTGGAACCAGAAGAAGCGGTGGCGTTGATGTCTGCCTTTGTGTTTCAGCAGAAGAATACTTCTGAACCTTCTCTTACTCCAAAACTGTCTCAGGCCAAACAGAGGTTGTACGATATAGCAATAAGACTTGGCCAGCTTCAATCCCACTTCAAACTTCAAATAAATCCAGAGGAGTACGCTCGAGAGAATCTCAAGTTTGGTCTTGTTGAAGTCGTTTATGAGTGGGCGAAGGGCACTCCGTTTGCAGACATTTGTGAACTTACAGATGTTGCTGAAGGAATGATAGTGCGAACCATTGTCAGACTTGATGAGACGTGTCGCGAGTTCAAAAATGCTGCATCTATTATGGGTAATTCTGCTCTGTACAAGAAAATGGTAACAGCTTCAAATGCAATCAAGCGCGACATTGTATTTGCAGCTAGCTTATACGTCACCGGAGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000184 GO:0000288 GO:0000291 GO:0000956 GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0006139 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010035 GO:0010038 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0015672 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0019439 GO:0030001 GO:0032991 GO:0034427 GO:0034641 GO:0034655 GO:0035864 GO:0040029 GO:0042221 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043928 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0048519 GO:0050789 GO:0050896 GO:0051179 GO:0051234 GO:0055087 GO:0060255 GO:0065007 GO:0070035 GO:0070478 GO:0071704 GO:0090304 GO:0140098 GO:1901360 GO:1901361 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko03018 map03018

Pfam Domains

Protein Families

DEAD DSHCT Helicase_C rRNA_proc-arch

Protein Analysis

1352

Amino Acids

150.5

Weight (kDa)

5.66

Isoelectric Point (pI)

44.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ski2_N	PF17911	141 - 215	9.6e-07	Ski2 N-terminal region
DEAD	PF00270	358 - 506	9.3e-23	DEAD/DEAH box helicase
Helicase_C	PF00271	714 - 793	9.2e-06	Helicase conserved C-terminal domain
MTR4-like_stalk	PF21408	848 - 912	1.9e-13	Exosome RNA helicase MTR4-like, stalk
MTR4_beta-barrel	PF13234	915 - 1147	3e-27	Mtr4-like, beta-barrel domain
DSHCT	PF08148	1173 - 1343	4.6e-59	DSHCT (NUC185) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0005110)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46960 AT3G46960 AT3G46960
fragaria_vesca	FvH4_3g37880 FvH4_3g37880 FvH4_3g37880 FvH4_3g37880 FvH4_3g37880
malus_domestica	MD03G1077900.v1.1 MD11G1082500.v1.1
prunus_persica	Prupe.6G062900_v2.0.a1 Prupe.6G062900_v2.0.a1 Prupe.6G062900_v2.0.a1
pyrus_communis	pycom03g06210 pycom11g07060
rosa_chinensis	RchiOBHm_Chr5g0068091
rosa_laevigata	RLG00000035954
rosa_multiflora	Rmu_sc0000435.1_g000017
rosa_roxburghii	Rroxscaffold_1G00012520 Rroxscaffold_1G00013260
rosa_rugosa	Rorug05G0387900 Rorug05G0388300 Rorug05G0388400 Rorug05G0388500
rosa_samantha	Rh5AG446500 Rh5BG464600 Rh5CG486400 Rh5DG478400
rosa_wichuraiana	Rw5G041700 Rw5G041740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1457
AarI	CACCTGC	1 cut(s)	382
Acc36I	ACCTGC	1 cut(s)	382
Acc65I	GGTACC	1 cut(s)	1501
AccB1I	GGYRCC	4 cut(s)	342, 1501, 1732, 2965
AccBSI	CCGCTC	1 cut(s)	2694
AccI	GTMKAC	1 cut(s)	1208
AccII	CGCG	6 cut(s)	304, 579, 1359, 2083, 3933, 4014
AccIII	TCCGGA	1 cut(s)	1613
AclWI	GGATC	9 cut(s)	12, 172, 693, 820, 1732, 2101, 2500, 3245, 3258
AcoI	YGGCCR	3 cut(s)	52, 61, 3742
AcsI	RAATTY	8 cut(s)	785, 1073, 1262, 1702, 1822, 1831, 2303, 2318
AcuI	CTGAAG	6 cut(s)	384, 671, 1725, 2555, 3287, 3906
AcvI	CACGTG	1 cut(s)	324
AcyI	GRCGYC	2 cut(s)	158, 343
AdeI	CACNNNGTG	2 cut(s)	2843, 3585
AfaI	GTAC	9 cut(s)	474, 576, 612, 1000, 1503, 3236, 3722, 3788, 3977
AfiI	CCNNNNNNNGG	4 cut(s)	88, 1084, 3059, 3714
AflII	CTTAAG	1 cut(s)	3422
AflIII	ACRYGT	1 cut(s)	3926
AjiI	CACGTC	2 cut(s)	708, 3927
AjnI	CCWGG	6 cut(s)	160, 505, 2335, 3057, 3246, 3612
AjuI	GAANNNNNNNTTGG	4 cut(s)	573, 605, 3141, 3173
Alw21I	GWGCWC	4 cut(s)	424, 1410, 1599, 3372
Alw26I	GTCTC	8 cut(s)	120, 149, 1322, 2237, 2748, 3391, 3705, 3917
Alw44I	GTGCAC	1 cut(s)	1406
AlwI	GGATC	9 cut(s)	12, 172, 693, 820, 1732, 2101, 2500, 3245, 3258
AlwNI	CAGNNNCTG	1 cut(s)	821
Ama87I	CYCGRG	3 cut(s)	423, 1655, 3792
Aor13HI	TCCGGA	1 cut(s)	1613
AoxI	GGCC	9 cut(s)	52, 61, 654, 1301, 2359, 3472, 3535, 3705, 3742
ApaLI	GTGCAC	1 cut(s)	1406
ApeKI	GCWGC	9 cut(s)	17, 306, 1127, 1670, 2602, 3068, 3179, 3947, 4028
ApoI	RAATTY	8 cut(s)	785, 1073, 1262, 1702, 1822, 1831, 2303, 2318
ArsI	GACNNNNNNTTYG	6 cut(s)	206, 238, 1103, 1135, 4007, 4039
Asp718I	GGTACC	1 cut(s)	1501
AspA2I	CCTAGG	2 cut(s)	149, 2425
AspLEI	GCGC	4 cut(s)	306, 345, 2806, 4014
AspS9I	GGNCC	4 cut(s)	455, 637, 1814, 2809
AsuC2I	CCSGG	3 cut(s)	38, 1567, 2470
AsuHPI	GGTGA	6 cut(s)	656, 962, 1298, 2672, 3088, 4036
AsuNHI	GCTAGC	1 cut(s)	4031
AvaI	CYCGRG	3 cut(s)	423, 1655, 3792
AvaII	GGWCC	4 cut(s)	455, 637, 1814, 2809
AvrII	CCTAGG	2 cut(s)	149, 2425
AxyI	CCTNAGG	1 cut(s)	603
BaeGI	GKGCMC	2 cut(s)	1410, 3848
BalI	TGGCCA	2 cut(s)	63, 3744
BamHI	GGATCC	2 cut(s)	4, 3250
BanI	GGYRCC	4 cut(s)	342, 1501, 1732, 2965
BanII	GRGCYC	4 cut(s)	30, 81, 424, 1762
BarI	GAAGNNNNNNTAC	2 cut(s)	567, 599
BauI	CACGAG	1 cut(s)	3287
BbrPI	CACGTG	1 cut(s)	324
Bbv12I	GWGCWC	4 cut(s)	424, 1410, 1599, 3372
BbvI	GCAGC	9 cut(s)	29, 293, 1114, 1657, 2614, 3055, 3166, 3934, 4040
BccI	CCATC	8 cut(s)	545, 791, 869, 1235, 1748, 2166, 2303, 3322
BceAI	ACGGC	3 cut(s)	2346, 2397, 3522
BcgI	CGANNNNNNTGC	4 cut(s)	206, 240, 2501, 2535
BciT130I	CCWGG	6 cut(s)	162, 507, 2337, 3059, 3248, 3614
BclI	TGATCA	1 cut(s)	2220
BcnI	CCSGG	3 cut(s)	38, 1567, 2470
BcoDI	GTCTC	8 cut(s)	120, 149, 1322, 2237, 2748, 3391, 3705, 3917
BfaI	CTAG	6 cut(s)	150, 533, 2274, 2426, 2841, 4032
BfmI	CTRYAG	1 cut(s)	3488
BfoI	RGCGCY	1 cut(s)	346
BfrI	CTTAAG	1 cut(s)	3422
BfuAI	ACCTGC	1 cut(s)	382
BglI	GCCNNNNNGGC	3 cut(s)	60, 340, 2367
BglII	AGATCT	2 cut(s)	935, 3262
BlnI	CCTAGG	2 cut(s)	149, 2425
Bme1390I	CCNGG	9 cut(s)	38, 162, 507, 1567, 2337, 2470, 3059, 3248, 3614
Bme18I	GGWCC	4 cut(s)	455, 637, 1814, 2809
BmeT110I	CYCGRG	3 cut(s)	423, 1655, 3792
BmgBI	CACGTC	2 cut(s)	708, 3927
BmgT120I	GGNCC	4 cut(s)	455, 637, 1814, 2809
BmrFI	CCNGG	9 cut(s)	38, 162, 507, 1567, 2337, 2470, 3059, 3248, 3614
BmrI	ACTGGG	3 cut(s)	1795, 2781, 2915
BmsI	GCATC	8 cut(s)	492, 1190, 1669, 1894, 2521, 2604, 3406, 3959
BmtI	GCTAGC	1 cut(s)	4035
BmuI	ACTGGG	3 cut(s)	1795, 2781, 2915
BplI	GAGNNNNNCTC	4 cut(s)	68, 100, 3786, 3818
BpmI	CTGGAG	1 cut(s)	3225
Bpu10I	CCTNAGC	2 cut(s)	462, 2628
BpuEI	CTTGAG	3 cut(s)	95, 1318, 3788
BpuMI	CCSGG	3 cut(s)	38, 1567, 2470
Bsa29I	ATCGAT	1 cut(s)	864
BsaAI	YACGTR	2 cut(s)	324, 1376
BsaBI	GATNNNNATC	2 cut(s)	817, 3282
BsaHI	GRCGYC	2 cut(s)	158, 343
BsaWI	WCCGGW	2 cut(s)	1613, 4046
BsaXI	ACNNNNNCTCC	2 cut(s)	2814, 2844
Bsc4I	CCNNNNNNNGG	4 cut(s)	88, 1084, 3059, 3714
Bse21I	CCTNAGG	1 cut(s)	603
Bse3DI	GCAATG	4 cut(s)	437, 1053, 1189, 2259
Bse8I	GATNNNNATC	2 cut(s)	817, 3282
BseAI	TCCGGA	1 cut(s)	1613
BseBI	CCWGG	6 cut(s)	162, 507, 2337, 3059, 3248, 3614
BseCI	ATCGAT	1 cut(s)	864
BseJI	GATNNNNATC	2 cut(s)	817, 3282
BseLI	CCNNNNNNNGG	4 cut(s)	88, 1084, 3059, 3714
BseMI	GCAATG	4 cut(s)	437, 1053, 1189, 2259
BseRI	GAGGAG	6 cut(s)	200, 591, 1863, 2690, 3581, 3797
BseSI	GKGCMC	2 cut(s)	1410, 3848
BseXI	GCAGC	9 cut(s)	29, 293, 1114, 1657, 2614, 3055, 3166, 3934, 4040
BseYI	CCCAGC	1 cut(s)	3071
BsgI	GTGCAG	2 cut(s)	410, 758
Bsh1236I	CGCG	6 cut(s)	304, 579, 1359, 2083, 3933, 4014
BshFI	GGCC	9 cut(s)	54, 63, 656, 1303, 2361, 3474, 3537, 3707, 3744
BshNI	GGYRCC	4 cut(s)	342, 1501, 1732, 2965
BshVI	ATCGAT	1 cut(s)	864
BsiHKAI	GWGCWC	4 cut(s)	424, 1410, 1599, 3372
BsiHKCI	CYCGRG	3 cut(s)	423, 1655, 3792
BsiSI	CCGG	8 cut(s)	37, 51, 70, 1566, 1614, 2470, 3475, 4047
BslFI	GGGAC	5 cut(s)	1124, 1571, 1582, 1800, 2032
BslI	CCNNNNNNNGG	4 cut(s)	88, 1084, 3059, 3714
BsmAI	GTCTC	8 cut(s)	120, 149, 1322, 2237, 2748, 3391, 3705, 3917
BsmBI	CGTCTC	2 cut(s)	149, 3917
BsmFI	GGGAC	5 cut(s)	1124, 1571, 1582, 1800, 2032
BsnI	GGCC	9 cut(s)	54, 63, 656, 1303, 2361, 3474, 3537, 3707, 3744
BsoBI	CYCGRG	3 cut(s)	423, 1655, 3792
Bsp1286I	GDGCHC	8 cut(s)	30, 81, 424, 1410, 1599, 1762, 3372, 3848
Bsp13I	TCCGGA	1 cut(s)	1613
Bsp1407I	TGTACA	1 cut(s)	3975
Bsp19I	CCATGG	2 cut(s)	696, 3346
Bsp68I	TCGCGA	1 cut(s)	3933
BspANI	GGCC	9 cut(s)	54, 63, 656, 1303, 2361, 3474, 3537, 3707, 3744
BspDI	ATCGAT	1 cut(s)	864
BspEI	TCCGGA	1 cut(s)	1613
BspFNI	CGCG	6 cut(s)	304, 579, 1359, 2083, 3933, 4014
BspHI	TCATGA	2 cut(s)	1024, 2787
BspMAI	CTGCAG	1 cut(s)	3492
BspMI	ACCTGC	1 cut(s)	382
BspOI	GCTAGC	1 cut(s)	4035
BspPI	GGATC	9 cut(s)	12, 172, 693, 820, 1732, 2101, 2500, 3245, 3258
BspT107I	GGYRCC	4 cut(s)	342, 1501, 1732, 2965
BspTI	CTTAAG	1 cut(s)	3422
BsrBI	CCGCTC	1 cut(s)	2694
BsrDI	GCAATG	4 cut(s)	437, 1053, 1189, 2259
BsrGI	TGTACA	1 cut(s)	3975
BssNAI	GTATAC	1 cut(s)	1209
BssNI	GRCGYC	2 cut(s)	158, 343
BssSI	CACGAG	1 cut(s)	3287
BssT1I	CCWWGG	6 cut(s)	149, 285, 640, 696, 2425, 3346
Bst1107I	GTATAC	1 cut(s)	1209
Bst2BI	CACGAG	1 cut(s)	3287
Bst2UI	CCWGG	6 cut(s)	162, 507, 2337, 3059, 3248, 3614
Bst6I	CTCTTC	6 cut(s)	765, 948, 2368, 2988, 3159, 3389
BstACI	GRCGYC	2 cut(s)	158, 343
BstAFI	CTTAAG	1 cut(s)	3422
BstAPI	GCANNNNNTGC	4 cut(s)	1166, 1679, 2363, 2843
BstAUI	TGTACA	1 cut(s)	3975
BstBAI	YACGTR	2 cut(s)	324, 1376
BstC8I	GCNNGC	7 cut(s)	141, 2359, 2368, 2625, 3547, 3746, 4033
BstDSI	CCRYGG	3 cut(s)	87, 696, 3346
BstFNI	CGCG	6 cut(s)	304, 579, 1359, 2083, 3933, 4014
BstH2I	RGCGCY	1 cut(s)	346
BstHHI	GCGC	4 cut(s)	306, 345, 2806, 4014
BstMAI	GTCTC	8 cut(s)	120, 149, 1322, 2237, 2748, 3391, 3705, 3917
BstNI	CCWGG	6 cut(s)	162, 507, 2337, 3059, 3248, 3614
BstNSI	RCATGY	2 cut(s)	2450, 2551
BstSCI	CCNGG	9 cut(s)	36, 160, 505, 1565, 2335, 2468, 3057, 3246, 3612
BstSFI	CTRYAG	1 cut(s)	3488
BstSLI	GKGCMC	2 cut(s)	1410, 3848
BstUI	CGCG	6 cut(s)	304, 579, 1359, 2083, 3933, 4014
BstV1I	GCAGC	9 cut(s)	29, 293, 1114, 1657, 2614, 3055, 3166, 3934, 4040
BstX2I	RGATCY	5 cut(s)	4, 935, 2093, 3250, 3262
BstXI	CCANNNNNNTGG	4 cut(s)	353, 669, 2165, 2819
BstYI	RGATCY	5 cut(s)	4, 935, 2093, 3250, 3262
BstZ17I	GTATAC	1 cut(s)	1209
Bsu15I	ATCGAT	1 cut(s)	864
Bsu36I	CCTNAGG	1 cut(s)	603
BsuRI	GGCC	9 cut(s)	54, 63, 656, 1303, 2361, 3474, 3537, 3707, 3744
BsuTUI	ATCGAT	1 cut(s)	864
BtgI	CCRYGG	3 cut(s)	87, 696, 3346
BtrI	CACGTC	2 cut(s)	708, 3927
BtsI	GCAGTG	3 cut(s)	254, 296, 3181
BtsIMutI	CAGTG	9 cut(s)	64, 254, 296, 1281, 1597, 1710, 2903, 3181, 3409
BtuMI	TCGCGA	1 cut(s)	3933
BveI	ACCTGC	1 cut(s)	382
Cac8I	GCNNGC	7 cut(s)	141, 2359, 2368, 2625, 3547, 3746, 4033
CaiI	CAGNNNCTG	1 cut(s)	821
CciI	TCATGA	2 cut(s)	1024, 2787
CfoI	GCGC	4 cut(s)	306, 345, 2806, 4014
Cfr13I	GGNCC	4 cut(s)	455, 637, 1814, 2809
ClaI	ATCGAT	1 cut(s)	864
CseI	GACGC	1 cut(s)	166
Csp6I	GTAC	9 cut(s)	473, 575, 611, 999, 1502, 3235, 3721, 3787, 3976
CviQI	GTAC	9 cut(s)	473, 575, 611, 999, 1502, 3235, 3721, 3787, 3976
DinI	GGCGCC	1 cut(s)	344
DraIII	CACNNNGTG	2 cut(s)	2843, 3585
EaeI	YGGCCR	3 cut(s)	52, 61, 3742
Eam1104I	CTCTTC	6 cut(s)	765, 948, 2368, 2988, 3159, 3389
EarI	CTCTTC	6 cut(s)	765, 948, 2368, 2988, 3159, 3389
EciI	GGCGGA	1 cut(s)	1806
Ecl136II	GAGCTC	1 cut(s)	422
Eco130I	CCWWGG	6 cut(s)	149, 285, 640, 696, 2425, 3346
Eco147I	AGGCCT	1 cut(s)	656
Eco24I	GRGCYC	4 cut(s)	30, 81, 424, 1762
Eco47I	GGWCC	4 cut(s)	455, 637, 1814, 2809
Eco53kI	GAGCTC	1 cut(s)	422
Eco57I	CTGAAG	6 cut(s)	384, 671, 1725, 2555, 3287, 3906
Eco72I	CACGTG	1 cut(s)	324
Eco81I	CCTNAGG	1 cut(s)	603
Eco88I	CYCGRG	3 cut(s)	423, 1655, 3792
EcoICRI	GAGCTC	1 cut(s)	422
EcoRI	GAATTC	1 cut(s)	1702
EcoRII	CCWGG	6 cut(s)	160, 505, 2335, 3057, 3246, 3612
EcoT14I	CCWWGG	6 cut(s)	149, 285, 640, 696, 2425, 3346
EcoT22I	ATGCAT	3 cut(s)	1171, 1684, 3122
EcoT38I	GRGCYC	4 cut(s)	30, 81, 424, 1762
EgeI	GGCGCC	1 cut(s)	344
EheI	GGCGCC	1 cut(s)	344
ErhI	CCWWGG	6 cut(s)	149, 285, 640, 696, 2425, 3346
Esp3I	CGTCTC	2 cut(s)	149, 3917
FalI	AAGNNNNNCTT	2 cut(s)	3141, 3173
FaqI	GGGAC	5 cut(s)	1124, 1571, 1582, 1800, 2032
FauI	CCCGC	1 cut(s)	2359
FauNDI	CATATG	1 cut(s)	3122
FbaI	TGATCA	1 cut(s)	2220
FblI	GTMKAC	1 cut(s)	1208
FriOI	GRGCYC	4 cut(s)	30, 81, 424, 1762
FspBI	CTAG	6 cut(s)	150, 533, 2274, 2426, 2841, 4032
GlaI	GCGC	4 cut(s)	305, 344, 2805, 4013
GsaI	CCCAGC	1 cut(s)	3075
GsuI	CTGGAG	1 cut(s)	3225
HaeII	RGCGCY	1 cut(s)	346
HaeIII	GGCC	9 cut(s)	54, 63, 656, 1303, 2361, 3474, 3537, 3707, 3744
HapII	CCGG	8 cut(s)	37, 51, 70, 1566, 1614, 2470, 3475, 4047
HgaI	GACGC	1 cut(s)	166
HhaI	GCGC	4 cut(s)	306, 345, 2806, 4014
Hin1I	GRCGYC	2 cut(s)	158, 343
Hin6I	GCGC	4 cut(s)	304, 343, 2804, 4012
HinP1I	GCGC	4 cut(s)	304, 343, 2804, 4012
HincII	GTYRAC	1 cut(s)	3100
HindII	GTYRAC	1 cut(s)	3100
HindIII	AAGCTT	3 cut(s)	619, 1645, 2621
HpaII	CCGG	8 cut(s)	37, 51, 70, 1566, 1614, 2470, 3475, 4047
HphI	GGTGA	6 cut(s)	656, 962, 1298, 2672, 3088, 4036
Hpy166II	GTNNAC	6 cut(s)	1209, 1408, 2663, 3100, 3309, 3869
Hpy8I	GTNNAC	6 cut(s)	1209, 1408, 2663, 3100, 3309, 3869
Hpy99I	CGWCG	2 cut(s)	2831, 3227
HpyCH4IV	ACGT	7 cut(s)	323, 445, 707, 1375, 2980, 3926, 4041
HpySE526I	ACGT	7 cut(s)	323, 445, 707, 1375, 2980, 3926, 4041
Hsp92I	GRCGYC	2 cut(s)	158, 343
HspAI	GCGC	4 cut(s)	304, 343, 2804, 4012
KasI	GGCGCC	1 cut(s)	342
Kpn2I	TCCGGA	1 cut(s)	1613
KpnI	GGTACC	1 cut(s)	1505
Ksp22I	TGATCA	1 cut(s)	2220
LmnI	GCTCC	5 cut(s)	104, 289, 419, 1219, 1594
Lsp1109I	GCAGC	9 cut(s)	29, 293, 1114, 1657, 2614, 3055, 3166, 3934, 4040
LweI	GCATC	8 cut(s)	492, 1190, 1669, 1894, 2521, 2604, 3406, 3959
MaeI	CTAG	6 cut(s)	150, 533, 2274, 2426, 2841, 4032
MaeII	ACGT	7 cut(s)	323, 445, 707, 1375, 2980, 3926, 4041
MaeIII	GTNAC	6 cut(s)	1561, 1920, 2066, 2915, 3988, 4042
MbiI	CCGCTC	1 cut(s)	2694
MfeI	CAATTG	3 cut(s)	803, 2178, 3127
MflI	RGATCY	5 cut(s)	4, 935, 2093, 3250, 3262
MhlI	GDGCHC	8 cut(s)	30, 81, 424, 1410, 1599, 1762, 3372, 3848
MlsI	TGGCCA	2 cut(s)	63, 3744
MluNI	TGGCCA	2 cut(s)	63, 3744
Mly113I	GGCGCC	1 cut(s)	343
MlyI	GAGTC	5 cut(s)	437, 1106, 2807, 2832, 2893
MmeI	TCCRAC	8 cut(s)	22, 126, 873, 1252, 2437, 3189, 3246, 3342
Mox20I	TGGCCA	2 cut(s)	63, 3744
Mph1103I	ATGCAT	3 cut(s)	1171, 1684, 3122
MroI	TCCGGA	1 cut(s)	1613
MscI	TGGCCA	2 cut(s)	63, 3744
MslI	CAYNNNNRTG	2 cut(s)	667, 2351
Msp20I	TGGCCA	2 cut(s)	63, 3744
MspA1I	CMGCKG	3 cut(s)	1142, 2003, 3205
MspCI	CTTAAG	1 cut(s)	3422
MspI	CCGG	8 cut(s)	37, 51, 70, 1566, 1614, 2470, 3475, 4047
MspR9I	CCNGG	9 cut(s)	38, 162, 507, 1567, 2337, 2470, 3059, 3248, 3614
MunI	CAATTG	3 cut(s)	803, 2178, 3127
MvaI	CCWGG	6 cut(s)	162, 507, 2337, 3059, 3248, 3614
MvnI	CGCG	6 cut(s)	304, 579, 1359, 2083, 3933, 4014
NarI	GGCGCC	1 cut(s)	343
NciI	CCSGG	3 cut(s)	38, 1567, 2470
NcoI	CCATGG	2 cut(s)	696, 3346
NdeI	CATATG	1 cut(s)	3122
NheI	GCTAGC	1 cut(s)	4031
NmuCI	GTSAC	3 cut(s)	1920, 2066, 4042
NruI	TCGCGA	1 cut(s)	3933
NsiI	ATGCAT	3 cut(s)	1171, 1684, 3122
NspI	RCATGY	2 cut(s)	2450, 2551
PaeR7I	CTCGAG	2 cut(s)	423, 3792
PagI	TCATGA	2 cut(s)	1024, 2787
PaqCI	CACCTGC	1 cut(s)	382
PceI	AGGCCT	1 cut(s)	656
PflFI	GACNNNGTC	1 cut(s)	107
PfoI	TCCNGGA	2 cut(s)	505, 2335
PleI	GAGTC	5 cut(s)	436, 1106, 2806, 2831, 2893
PluTI	GGCGCC	1 cut(s)	346
PmaCI	CACGTG	1 cut(s)	324
PmlI	CACGTG	1 cut(s)	324
PpsI	GAGTC	5 cut(s)	436, 1106, 2806, 2831, 2893
Ppu21I	YACGTR	2 cut(s)	324, 1376
PsiI	TTATAA	1 cut(s)	1457
Psp124BI	GAGCTC	1 cut(s)	424
Psp6I	CCWGG	6 cut(s)	160, 505, 2335, 3057, 3246, 3612
PspCI	CACGTG	1 cut(s)	324
PspFI	CCCAGC	1 cut(s)	3071
PspGI	CCWGG	6 cut(s)	160, 505, 2335, 3057, 3246, 3612
PspPI	GGNCC	4 cut(s)	455, 637, 1814, 2809
PstI	CTGCAG	1 cut(s)	3492
PstNI	CAGNNNCTG	1 cut(s)	821
PsuI	RGATCY	5 cut(s)	4, 935, 2093, 3250, 3262
PsyI	GACNNNGTC	1 cut(s)	107
PvuII	CAGCTG	3 cut(s)	1142, 2003, 3205
RruI	TCGCGA	1 cut(s)	3933
RsaI	GTAC	9 cut(s)	474, 576, 612, 1000, 1503, 3236, 3722, 3788, 3977
RsaNI	GTAC	9 cut(s)	473, 575, 611, 999, 1502, 3235, 3721, 3787, 3976
RseI	CAYNNNNRTG	2 cut(s)	667, 2351
SacI	GAGCTC	1 cut(s)	424
Sau96I	GGNCC	4 cut(s)	455, 637, 1814, 2809
SchI	GAGTC	5 cut(s)	437, 1106, 2807, 2832, 2893
ScrFI	CCNGG	9 cut(s)	38, 162, 507, 1567, 2337, 2470, 3059, 3248, 3614
SduI	GDGCHC	8 cut(s)	30, 81, 424, 1410, 1599, 1762, 3372, 3848
SfaNI	GCATC	8 cut(s)	492, 1190, 1669, 1894, 2521, 2604, 3406, 3959
SfcI	CTRYAG	1 cut(s)	3488
SfiI	GGCCNNNNNGGCC	1 cut(s)	60
SfoI	GGCGCC	1 cut(s)	344
Sfr274I	CTCGAG	2 cut(s)	423, 3792
SinI	GGWCC	4 cut(s)	455, 637, 1814, 2809
SlaI	CTCGAG	2 cut(s)	423, 3792
SmiMI	CAYNNNNRTG	2 cut(s)	667, 2351
SmlI	CTYRAG	6 cut(s)	74, 423, 1297, 3422, 3792, 3803
SmoI	CTYRAG	6 cut(s)	74, 423, 1297, 3422, 3792, 3803
SseBI	AGGCCT	1 cut(s)	656
SspDI	GGCGCC	1 cut(s)	342
SspI	AATATT	3 cut(s)	1338, 1480, 2724
SspMI	CTAG	6 cut(s)	150, 533, 2274, 2426, 2841, 4032
SstI	GAGCTC	1 cut(s)	424
StuI	AGGCCT	1 cut(s)	656
StyD4I	CCNGG	9 cut(s)	36, 160, 505, 1565, 2335, 2468, 3057, 3246, 3612
StyI	CCWWGG	6 cut(s)	149, 285, 640, 696, 2425, 3346
TaiI	ACGT	7 cut(s)	326, 448, 710, 1378, 2983, 3929, 4044
TatI	WGTACW	1 cut(s)	3975
TauI	GCSGC	4 cut(s)	139, 304, 2086, 2896
TscAI	CASTG	9 cut(s)	64, 254, 303, 1288, 1604, 1717, 2910, 3181, 3409
TseFI	GTSAC	3 cut(s)	1920, 2066, 4042
TseI	GCWGC	9 cut(s)	17, 306, 1127, 1670, 2602, 3068, 3179, 3947, 4028
Tsp45I	GTSAC	3 cut(s)	1920, 2066, 4042
TspGWI	ACGGA	5 cut(s)	1547, 1552, 2732, 2954, 3840
TspRI	CASTG	9 cut(s)	64, 254, 303, 1288, 1604, 1717, 2910, 3181, 3409
Tth111I	GACNNNGTC	1 cut(s)	107
Vha464I	CTTAAG	1 cut(s)	3422
VneI	GTGCAC	1 cut(s)	1406
VpaK11BI	GGWCC	4 cut(s)	455, 637, 1814, 2809
XapI	RAATTY	8 cut(s)	785, 1073, 1262, 1702, 1822, 1831, 2303, 2318
XbaI	TCTAGA	1 cut(s)	532
XceI	RCATGY	2 cut(s)	2450, 2551
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1045
XhoI	CTCGAG	2 cut(s)	423, 3792
XmaJI	CCTAGG	2 cut(s)	149, 2425
XmiI	GTMKAC	1 cut(s)	1208
XspI	CTAG	6 cut(s)	150, 533, 2274, 2426, 2841, 4032
Zsp2I	ATGCAT	3 cut(s)	1171, 1684, 3122

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.