MD03G1082700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010345
Physical Location & Seq
Reverse (-)
6710930 .. 6714579
3650 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1082700.v1.1.491

Sequence Viewer

Length: 1158 bp
ATGATGGATTTTGAGTGCTCCAAACCAGATACCAAGGCCTGGGATATTCGTGAAGCAGCTGCTAACTATAATTACAAAGGACTTATTGGCTCATCAGATTTGAGTTCAACTTCTTCATCTTCAGCACTTGAGGATTCTGAACTTGTTTCATCTGATCAGTGGACAGAGACTACTACTGATGGGGACGACGATTACGTTGCAGAATTAACTCGCCAGATGACTAACTACATGCTTCAAGATGATCACGACAAAACCAATACTACTAGCTCTCAGAAAAACCAAGAGTTATTGGGTTATGTTGGTTGGCCACAGTACTCAGTGTGGCCACCGTTGGGGTCTAGCCATGGCAGCTCTGAAGGGTCATCTCTTGAACCAACGCCACCAGAAACACCAGGCAAAGACAAAGCCTGGAACAGGTGTACCTACGCCGACGTTTTAAGAAAGTTGGAGAAGTTAGATACAACTACAACCAACGAAGGGTCACATCATCCTCAGGGAATAAACGCAAGTACAAAAGTAGACCCAGGTGTTGGATTTTGCTCAAAGCAAGATCTAGCTTCCAGTCAGAGCCAAGATTTTCAGAAACTTGAGGCCTGTAATTATAAGCTAAAGCAAGAGCACATTTCAAGGAAACAAGGATCGTCAGGGAATAGAGGAAGACAAGCCAATATTTGCCATGAGCAATATGAGCCTAATAATTATCAGCAGTTGAAGAAAGGAGGATCTTGTATTGGGTCTATTAATGGAGGAAGGTCAAGGTCTCCAACACAATTTGGATTGCCTGGTTCAAACCCATGGGTGAACCAGCAAGCACGACCGGGCGTAAAGGCTGTTTTCCTTGGCGGGTCGAGTACGAAAATTGGGTCATCTTCTGGGACAGGAGTATTTTTGCCTTGTGTTATTGGTAACACTTCACAGCCACCCAAGAAAAGAGGATGTTCCCCGGTTCTTATACCCGCAAAGGTAGTGCACGCCTTGAAAGTTCACTTTGACAGAGTTGGCGACCTACCTCGACCTAATGCTTCTGGTTTCTCTACACAAAATGATGATTTGAAGGGTGGTAGGGCAGACATGCATTCAACTAATAAACATCAGTCGCGGCCGGTGCCGTGCACGAACCAAAAGGAGATGGGTCTGCCTCAAGAATGGACGTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

386

Amino Acids

41.98

Weight (kDa)

6.79

Isoelectric Point (pI)

47.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010345)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G59050 AT5G59050 AT5G59050 AT5G59050
fragaria_vesca FvH4_3g37230 FvH4_3g37230
malus_domestica MD03G1082700.v1.1 MD11G1090800.v1.1
prunus_persica Prupe.6G067000_v2.0.a1
pyrus_communis pycom03g06580 pycom11g07550
rosa_chinensis RchiOBHm_Chr5g0066981
rosa_laevigata RLG00000035898
rosa_multiflora Rmu_sc0008378.1_g000002
rosa_roxburghii Rroxscaffold_1G00014150
rosa_rugosa Rorug05G0380300
rosa_samantha Rh5AG439900 Rh5BG457000 Rh5CG479100 Rh5DG471700
rosa_wichuraiana Rw5G041140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 603
AccB1I GGYRCC 1 cut(s) 1105
AccB7I CCANNNNNTGG 2 cut(s) 39, 530
AccI GTMKAC 1 cut(s) 519
AccII CGCG 1 cut(s) 1099
AciI CCGC 3 cut(s) 843, 957, 1099
AclWI GGATC 2 cut(s) 646, 730
AcoI YGGCCR 3 cut(s) 305, 323, 1100
AcuI CTGAAG 2 cut(s) 105, 375
AfaI GTAC 4 cut(s) 314, 421, 511, 853
AfiI CCNNNNNNNGG 5 cut(s) 39, 332, 414, 477, 530
AgsI TTSAA 9 cut(s) 108, 236, 371, 627, 712, 789, 979, 1054, 1080
AjnI CCWGG 5 cut(s) 38, 391, 407, 523, 781
AluBI AGCT 5 cut(s) 59, 267, 351, 557, 607
AluI AGCT 5 cut(s) 59, 267, 351, 557, 607
Alw21I GWGCWC 4 cut(s) 20, 621, 972, 1115
Alw26I GTCTC 2 cut(s) 161, 765
Alw44I GTGCAC 2 cut(s) 968, 1111
AlwI GGATC 2 cut(s) 646, 730
AoxI GGCC 5 cut(s) 36, 305, 323, 591, 1100
ApaLI GTGCAC 2 cut(s) 968, 1111
ApeKI GCWGC 3 cut(s) 56, 59, 348
ArsI GACNNNNNNTTYG 2 cut(s) 848, 880
AseI ATTAAT 1 cut(s) 741
AsuC2I CCSGG 2 cut(s) 819, 944
AsuHPI GGTGA 1 cut(s) 811
AxyI CCTNAGG 1 cut(s) 492
BaeGI GKGCMC 2 cut(s) 972, 1115
BaeI ACNNNNGTAYC 2 cut(s) 403, 436
BalI TGGCCA 2 cut(s) 307, 325
BanI GGYRCC 1 cut(s) 1105
BbsI GAAGAC 1 cut(s) 664
Bbv12I GWGCWC 4 cut(s) 20, 621, 972, 1115
BbvI GCAGC 3 cut(s) 46, 68, 360
BccI CCATC 2 cut(s) 173, 1123
BceAI ACGGC 1 cut(s) 1093
BcgI CGANNNNNNTGC 2 cut(s) 179, 213
BciT130I CCWGG 5 cut(s) 40, 393, 409, 525, 783
BclI TGATCA 2 cut(s) 154, 241
BcnI CCSGG 2 cut(s) 819, 944
BcoDI GTCTC 2 cut(s) 161, 765
BfaI CTAG 3 cut(s) 264, 339, 554
BglII AGATCT 1 cut(s) 550
BisI GCNGC 4 cut(s) 57, 60, 349, 1100
BlsI GCNGC 4 cut(s) 58, 61, 350, 1101
BmcAI AGTACT 1 cut(s) 314
Bme1390I CCNGG 7 cut(s) 40, 393, 409, 525, 783, 819, 944
BmiI GGNNCC 1 cut(s) 1107
BmrFI CCNGG 7 cut(s) 40, 393, 409, 525, 783, 819, 944
BpiI GAAGAC 1 cut(s) 664
BpuEI CTTGAG 3 cut(s) 149, 608, 1125
BpuMI CCSGG 2 cut(s) 819, 944
BsaI GGTCTC 1 cut(s) 765
BsaJI CCNNGG 7 cut(s) 33, 39, 343, 523, 794, 838, 942
Bsc4I CCNNNNNNNGG 5 cut(s) 39, 332, 414, 477, 530
Bse118I RCCGGY 1 cut(s) 1102
Bse1I ACTGG 1 cut(s) 561
Bse21I CCTNAGG 1 cut(s) 492
BseBI CCWGG 5 cut(s) 40, 393, 409, 525, 783
BseDI CCNNGG 7 cut(s) 33, 39, 343, 523, 794, 838, 942
BseGI GGATG 2 cut(s) 487, 941
BseLI CCNNNNNNNGG 5 cut(s) 39, 332, 414, 477, 530
BseMII CTCAG 3 cut(s) 284, 330, 506
BseNI ACTGG 1 cut(s) 561
BseSI GKGCMC 2 cut(s) 972, 1115
BseX3I CGGCCG 1 cut(s) 1100
BseXI GCAGC 3 cut(s) 46, 68, 360
Bsh1236I CGCG 1 cut(s) 1099
Bsh1285I CGRYCG 2 cut(s) 818, 1103
BshFI GGCC 5 cut(s) 38, 307, 325, 593, 1102
BshNI GGYRCC 1 cut(s) 1105
BsiEI CGRYCG 2 cut(s) 818, 1103
BsiHKAI GWGCWC 4 cut(s) 20, 621, 972, 1115
BsiSI CCGG 3 cut(s) 818, 944, 1103
BslFI GGGAC 2 cut(s) 197, 889
BslI CCNNNNNNNGG 5 cut(s) 39, 332, 414, 477, 530
BsmAI GTCTC 2 cut(s) 161, 765
BsmFI GGGAC 2 cut(s) 197, 889
BsmI GAATGC 1 cut(s) 1075
BsnI GGCC 5 cut(s) 38, 307, 325, 593, 1102
Bso31I GGTCTC 1 cut(s) 765
Bsp1286I GDGCHC 4 cut(s) 20, 621, 972, 1115
Bsp143I GATC 5 cut(s) 154, 241, 550, 638, 722
Bsp19I CCATGG 2 cut(s) 343, 794
BspACI CCGC 3 cut(s) 843, 957, 1099
BspANI GGCC 5 cut(s) 38, 307, 325, 593, 1102
BspCNI CTCAG 3 cut(s) 283, 329, 505
BspFNI CGCG 1 cut(s) 1099
BspLI GGNNCC 1 cut(s) 1107
BspPI GGATC 2 cut(s) 646, 730
BspT107I GGYRCC 1 cut(s) 1105
BspTNI GGTCTC 1 cut(s) 765
BsrFI RCCGGY 1 cut(s) 1102
BsrI ACTGG 1 cut(s) 561
BssAI RCCGGY 1 cut(s) 1102
BssECI CCNNGG 7 cut(s) 33, 39, 343, 523, 794, 838, 942
BssMI GATC 5 cut(s) 154, 241, 550, 638, 722
BssT1I CCWWGG 4 cut(s) 33, 343, 794, 838
Bst2UI CCWGG 5 cut(s) 40, 393, 409, 525, 783
Bst4CI ACNGT 2 cut(s) 312, 330
BstC8I GCNNGC 2 cut(s) 810, 972
BstDEI CTNAG 3 cut(s) 270, 316, 492
BstDSI CCRYGG 2 cut(s) 343, 794
BstENI CCTNNNNNAGG 1 cut(s) 412
BstF5I GGATG 2 cut(s) 487, 941
BstFNI CGCG 1 cut(s) 1099
BstKTI GATC 5 cut(s) 157, 244, 553, 641, 725
BstMAI GTCTC 2 cut(s) 161, 765
BstMBI GATC 5 cut(s) 154, 241, 550, 638, 722
BstMCI CGRYCG 2 cut(s) 818, 1103
BstMWI GCNNNNNNNGC 3 cut(s) 348, 688, 1105
BstNI CCWGG 5 cut(s) 40, 393, 409, 525, 783
BstNSI RCATGY 2 cut(s) 232, 1075
BstSCI CCNGG 7 cut(s) 38, 391, 407, 523, 781, 817, 942
BstSLI GKGCMC 2 cut(s) 972, 1115
BstUI CGCG 1 cut(s) 1099
BstV1I GCAGC 3 cut(s) 46, 68, 360
BstV2I GAAGAC 1 cut(s) 664
BstX2I RGATCY 2 cut(s) 550, 722
BstYI RGATCY 2 cut(s) 550, 722
BstZI CGGCCG 1 cut(s) 1100
Bsu36I CCTNAGG 1 cut(s) 492
BsuRI GGCC 5 cut(s) 38, 307, 325, 593, 1102
BtgI CCRYGG 2 cut(s) 343, 794
BtsCI GGATG 2 cut(s) 487, 941
BtsIMutI CAGTG 2 cut(s) 164, 324
Cac8I GCNNGC 2 cut(s) 810, 972
Cfr10I RCCGGY 1 cut(s) 1102
Csp6I GTAC 4 cut(s) 313, 420, 510, 852
CviAII CATG 5 cut(s) 229, 344, 677, 795, 1072
CviQI GTAC 4 cut(s) 313, 420, 510, 852
DdeI CTNAG 3 cut(s) 270, 316, 492
DpnI GATC 5 cut(s) 156, 243, 552, 640, 724
DpnII GATC 5 cut(s) 154, 241, 550, 638, 722
EaeI YGGCCR 3 cut(s) 305, 323, 1100
EagI CGGCCG 1 cut(s) 1100
EclXI CGGCCG 1 cut(s) 1100
Eco130I CCWWGG 4 cut(s) 33, 343, 794, 838
Eco147I AGGCCT 2 cut(s) 38, 593
Eco31I GGTCTC 1 cut(s) 765
Eco52I CGGCCG 1 cut(s) 1100
Eco57I CTGAAG 2 cut(s) 105, 375
Eco81I CCTNAGG 1 cut(s) 492
EcoNI CCTNNNNNAGG 1 cut(s) 412
EcoRII CCWGG 5 cut(s) 38, 391, 407, 523, 781
EcoT14I CCWWGG 4 cut(s) 33, 343, 794, 838
EcoT22I ATGCAT 1 cut(s) 1077
ErhI CCWWGG 4 cut(s) 33, 343, 794, 838
FaeI CATG 5 cut(s) 232, 347, 680, 798, 1075
FaqI GGGAC 2 cut(s) 197, 889
FatI CATG 5 cut(s) 228, 343, 676, 794, 1071
FauI CCCGC 2 cut(s) 836, 964
FbaI TGATCA 2 cut(s) 154, 241
FblI GTMKAC 1 cut(s) 519
Fnu4HI GCNGC 4 cut(s) 57, 60, 349, 1100
FokI GGATG 2 cut(s) 474, 948
Fsp4HI GCNGC 4 cut(s) 57, 60, 349, 1100
FspBI CTAG 3 cut(s) 264, 339, 554
GluI GCNGC 4 cut(s) 57, 60, 349, 1100
HaeIII GGCC 5 cut(s) 38, 307, 325, 593, 1102
HapII CCGG 3 cut(s) 818, 944, 1103
Hin1II CATG 5 cut(s) 232, 347, 680, 798, 1075
HinfI GANTC 1 cut(s) 134
HpaII CCGG 3 cut(s) 818, 944, 1103
HphI GGTGA 1 cut(s) 811
Hpy166II GTNNAC 7 cut(s) 162, 420, 520, 802, 970, 985, 1113
Hpy188I TCNGA 7 cut(s) 97, 139, 154, 273, 355, 567, 582
Hpy188III TCNNGA 5 cut(s) 50, 236, 245, 368, 1142
Hpy8I GTNNAC 7 cut(s) 162, 420, 520, 802, 970, 985, 1113
Hpy99I CGWCG 2 cut(s) 191, 434
HpyAV CCTTC 4 cut(s) 350, 470, 744, 1048
HpyCH4III ACNGT 2 cut(s) 312, 330
HpyCH4IV ACGT 3 cut(s) 195, 432, 1151
HpyCH4V TGCA 4 cut(s) 200, 970, 1075, 1113
HpyF10VI GCNNNNNNNGC 3 cut(s) 348, 688, 1105
HpyF3I CTNAG 3 cut(s) 270, 316, 492
HpySE526I ACGT 3 cut(s) 195, 432, 1151
Hsp92II CATG 5 cut(s) 232, 347, 680, 798, 1075
Ksp22I TGATCA 2 cut(s) 154, 241
Kzo9I GATC 5 cut(s) 154, 241, 550, 638, 722
LmnI GCTCC 1 cut(s) 23
Lsp1109I GCAGC 3 cut(s) 46, 68, 360
MaeI CTAG 3 cut(s) 264, 339, 554
MaeII ACGT 3 cut(s) 195, 432, 1151
MaeIII GTNAC 2 cut(s) 480, 905
MalI GATC 5 cut(s) 156, 243, 552, 640, 724
MboI GATC 5 cut(s) 154, 241, 550, 638, 722
MboII GAAGA 5 cut(s) 105, 111, 669, 724, 861
MflI RGATCY 2 cut(s) 550, 722
MhlI GDGCHC 4 cut(s) 20, 621, 972, 1115
MlsI TGGCCA 2 cut(s) 307, 325
MluCI AATT 6 cut(s) 70, 203, 598, 697, 770, 858
MluNI TGGCCA 2 cut(s) 307, 325
MmeI TCCRAC 3 cut(s) 426, 511, 788
MnlI CCTC 9 cut(s) 124, 501, 583, 647, 713, 740, 926, 1020, 1149
Mox20I TGGCCA 2 cut(s) 307, 325
Mph1103I ATGCAT 1 cut(s) 1077
MscI TGGCCA 2 cut(s) 307, 325
MseI TTAA 3 cut(s) 206, 437, 741
Msp20I TGGCCA 2 cut(s) 307, 325
MspA1I CMGCKG 1 cut(s) 59
MspI CCGG 3 cut(s) 818, 944, 1103
MspR9I CCNGG 7 cut(s) 40, 393, 409, 525, 783, 819, 944
Mva1269I GAATGC 1 cut(s) 1075
MvaI CCWGG 5 cut(s) 40, 393, 409, 525, 783
MvnI CGCG 1 cut(s) 1099
MwoI GCNNNNNNNGC 3 cut(s) 348, 688, 1105
NciI CCSGG 2 cut(s) 819, 944
NcoI CCATGG 2 cut(s) 343, 794
NdeII GATC 5 cut(s) 154, 241, 550, 638, 722
NlaIII CATG 5 cut(s) 232, 347, 680, 798, 1075
NlaIV GGNNCC 1 cut(s) 1107
NmuCI GTSAC 1 cut(s) 480
NsiI ATGCAT 1 cut(s) 1077
NspI RCATGY 2 cut(s) 232, 1075
PceI AGGCCT 2 cut(s) 38, 593
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PctI GAATGC 1 cut(s) 1075
PfeI GAWTC 1 cut(s) 134
PflMI CCANNNNNTGG 2 cut(s) 39, 530
PkrI GCNGC 4 cut(s) 58, 61, 350, 1101
PshBI ATTAAT 1 cut(s) 741
PsiI TTATAA 1 cut(s) 603
Psp6I CCWGG 5 cut(s) 38, 391, 407, 523, 781
PspGI CCWGG 5 cut(s) 38, 391, 407, 523, 781
PspN4I GGNNCC 1 cut(s) 1107
PsuI RGATCY 2 cut(s) 550, 722
PvuII CAGCTG 1 cut(s) 59
RsaI GTAC 4 cut(s) 314, 421, 511, 853
RsaNI GTAC 4 cut(s) 313, 420, 510, 852
SaqAI TTAA 3 cut(s) 206, 437, 741
SatI GCNGC 4 cut(s) 57, 60, 349, 1100
Sau3AI GATC 5 cut(s) 154, 241, 550, 638, 722
ScaI AGTACT 1 cut(s) 314
ScrFI CCNGG 7 cut(s) 40, 393, 409, 525, 783, 819, 944
SduI GDGCHC 4 cut(s) 20, 621, 972, 1115
SmlI CTYRAG 3 cut(s) 128, 587, 1140
SmoI CTYRAG 3 cut(s) 128, 587, 1140
Sse9I AATT 6 cut(s) 70, 203, 598, 697, 770, 858
SseBI AGGCCT 2 cut(s) 38, 593
SsiI CCGC 3 cut(s) 843, 957, 1099
SspI AATATT 1 cut(s) 670
SspMI CTAG 3 cut(s) 264, 339, 554
StuI AGGCCT 2 cut(s) 38, 593
StyD4I CCNGG 7 cut(s) 38, 391, 407, 523, 781, 817, 942
StyI CCWWGG 4 cut(s) 33, 343, 794, 838
TaaI ACNGT 2 cut(s) 312, 330
TaiI ACGT 3 cut(s) 198, 435, 1154
TaqI TCGA 2 cut(s) 848, 1012
TasI AATT 6 cut(s) 70, 203, 598, 697, 770, 858
TatI WGTACW 2 cut(s) 312, 509
TauI GCSGC 1 cut(s) 1102
TfiI GAWTC 1 cut(s) 134
Tru1I TTAA 3 cut(s) 206, 437, 741
Tru9I TTAA 3 cut(s) 206, 437, 741
TscAI CASTG 2 cut(s) 164, 324
TseFI GTSAC 1 cut(s) 480
TseI GCWGC 3 cut(s) 56, 59, 348
Tsp45I GTSAC 1 cut(s) 480
TspDTI ATGAA 2 cut(s) 105, 138
TspRI CASTG 2 cut(s) 164, 324
Van91I CCANNNNNTGG 2 cut(s) 39, 530
VneI GTGCAC 2 cut(s) 968, 1111
VspI ATTAAT 1 cut(s) 741
XagI CCTNNNNNAGG 1 cut(s) 412
XceI RCATGY 2 cut(s) 232, 1075
XmiI GTMKAC 1 cut(s) 519
XspI CTAG 3 cut(s) 264, 339, 554
ZrmI AGTACT 1 cut(s) 314
Zsp2I ATGCAT 1 cut(s) 1077
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.