Rh5BG457000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0010345
Physical Location & Seq
Forward (+)
73047549 .. 73051184
3636 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG457000.1

Sequence Viewer

Length: 960 bp
ATGATGGATGTTGTTGACTTCCAACACGGGAGAAAGTTACTGTTCGATCATCATCATTTTCTCAACGAGCAGGAAACTCCGTCGTCGGAGATGAAGTTTTCCGGTGGCTCCGATTACAAGCACCGGTGGGGTTTTCCTGAAGGTGAGGGGAAAGGGTTTGTTGGGTCGAGGACTACTGAAGCTGAGCTTGTTTCGTCGTCCGAGTCGACTGTAGAAGACGATGATTACATTGCTGAGTTGACTCGCCAGATGACTCACTACATGCTTCAAGACGACGACAAACACGCCCTGAAAACCAGTCCTACTAGCTCCGAGAAAAACGAAGAGTCGTGGGGTTGTGTCGGTTGGCCAAGTGACTCTACGATGATGTGGTCGCCTTTGGGATCGAGCAGTGGAAGCCCAGAAGGTCCATCTCAGGAGCCGTCGCCGCCGGTGACCCCAGGCAAATACCAAGCTTCGGAACATAAAAGTCTGTCGTACGCCGCCGTTTTGTGCAAGTTGGACAAGTTAGATATGAACAAACAGGGACGCCCAAGTGGAAGTACCCCAGTAGCTGAAGCTGAAAATCCAAAGCAATGTGTAGCTTTTGATCAAAGCCAAGACTCTAAGATTAATTTTACGCAAAAGCAATGCCAAATCTCAAAGAAGCAAGGCTCAATAAATAATGGTAGACAACCGAAGGATATTGCTCATCAAAAATTGAAGTCCTACGACCAAAACCAGAAGAAAGGAGGGTCTTCTGCTAATGGCGGATTAAGGCATCCTCCAGCTATTGCCAATTTACATTCAAACCCGTGGCTGAACAACCAGCAAGGCCCCTCAAATATGAGGGCAGTTTTCCTTGGTGGATCGGGTTCCGGTTCCAGAGTTGGGACGCCGTCTTGTGGGACTGGTGTCTTCTTGCCCTGTGTTATTGGTAACACATCCCAATCACCCAAGAATAAAAGAGGTACTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

319

Amino Acids

34.71

Weight (kDa)

6.83

Isoelectric Point (pI)

54.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010345)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G59050 AT5G59050 AT5G59050 AT5G59050
fragaria_vesca FvH4_3g37230 FvH4_3g37230
malus_domestica MD03G1082700.v1.1 MD11G1090800.v1.1
prunus_persica Prupe.6G067000_v2.0.a1
pyrus_communis pycom03g06580 pycom11g07550
rosa_chinensis RchiOBHm_Chr5g0066981
rosa_laevigata RLG00000035898
rosa_multiflora Rmu_sc0008378.1_g000002
rosa_roxburghii Rroxscaffold_1G00014150
rosa_rugosa Rorug05G0380300
rosa_samantha Rh5AG439900 Rh5BG457000 Rh5CG479100 Rh5DG471700
rosa_wichuraiana Rw5G041140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 206, 670
AciI CCGC 3 cut(s) 428, 483, 750
AclWI GGATC 2 cut(s) 391, 856
AcoI YGGCCR 1 cut(s) 347
AcuI CTGAAG 3 cut(s) 159, 198, 576
AcyI GRCGYC 2 cut(s) 529, 875
AfaI GTAC 3 cut(s) 479, 544, 952
AfiI CCNNNNNNNGG 3 cut(s) 457, 870, 884
AgeI ACCGGT 1 cut(s) 123
AgsI TTSAA 3 cut(s) 269, 703, 789
AjnI CCWGG 1 cut(s) 439
AluBI AGCT 8 cut(s) 182, 187, 309, 455, 554, 560, 584, 770
AluI AGCT 8 cut(s) 182, 187, 309, 455, 554, 560, 584, 770
AlwI GGATC 2 cut(s) 391, 856
AlwNI CAGNNNCTG 1 cut(s) 554
AoxI GGCC 2 cut(s) 347, 814
AseI ATTAAT 1 cut(s) 612
AsiGI ACCGGT 1 cut(s) 123
AspS9I GGNCC 2 cut(s) 407, 815
AsuHPI GGTGA 3 cut(s) 155, 445, 924
AvaII GGWCC 1 cut(s) 407
BalI TGGCCA 1 cut(s) 349
BbsI GAAGAC 3 cut(s) 222, 729, 889
BccI CCATC 1 cut(s) 418
BceAI ACGGC 3 cut(s) 406, 470, 862
BciT130I CCWGG 1 cut(s) 441
BclI TGATCA 1 cut(s) 589
BfaI CTAG 1 cut(s) 306
BfmI CTRYAG 1 cut(s) 210
BisI GCNGC 2 cut(s) 428, 483
BlpI GCTNAGC 1 cut(s) 183
BlsI GCNGC 2 cut(s) 429, 484
Bme1390I CCNGG 1 cut(s) 441
Bme18I GGWCC 1 cut(s) 407
BmgT120I GGNCC 2 cut(s) 407, 815
BmiI GGNNCC 5 cut(s) 109, 420, 817, 856, 862
BmrFI CCNGG 1 cut(s) 441
BmrI ACTGGG 1 cut(s) 542
BmsI GCATC 1 cut(s) 769
BmuI ACTGGG 1 cut(s) 542
BoxI GACNNNNGTC 1 cut(s) 893
BpiI GAAGAC 3 cut(s) 222, 729, 889
BpmI CTGGAG 1 cut(s) 750
Bpu1102I GCTNAGC 1 cut(s) 183
BsaBI GATNNNNATC 1 cut(s) 51
BsaHI GRCGYC 2 cut(s) 529, 875
BsaJI CCNNGG 3 cut(s) 439, 794, 841
BsaWI WCCGGW 3 cut(s) 101, 123, 857
BsaXI ACNNNNNCTCC 2 cut(s) 80, 110
Bsc4I CCNNNNNNNGG 3 cut(s) 457, 870, 884
Bse118I RCCGGY 2 cut(s) 123, 430
Bse1I ACTGG 3 cut(s) 297, 548, 895
Bse3DI GCAATG 3 cut(s) 228, 581, 635
Bse8I GATNNNNATC 1 cut(s) 51
BseBI CCWGG 1 cut(s) 441
BseDI CCNNGG 3 cut(s) 439, 794, 841
BseGI GGATG 3 cut(s) 13, 760, 923
BseJI GATNNNNATC 1 cut(s) 51
BseLI CCNNNNNNNGG 3 cut(s) 457, 870, 884
BseMI GCAATG 3 cut(s) 228, 581, 635
BseMII CTCAG 3 cut(s) 174, 225, 428
BseNI ACTGG 3 cut(s) 297, 548, 895
BshFI GGCC 2 cut(s) 349, 816
BshTI ACCGGT 1 cut(s) 123
BsiSI CCGG 4 cut(s) 102, 124, 431, 858
BsiWI CGTACG 1 cut(s) 477
BslFI GGGAC 3 cut(s) 540, 886, 901
BslI CCNNNNNNNGG 3 cut(s) 457, 870, 884
BsmFI GGGAC 3 cut(s) 540, 886, 901
BsnI GGCC 2 cut(s) 349, 816
Bsp143I GATC 4 cut(s) 46, 383, 589, 848
Bsp1720I GCTNAGC 1 cut(s) 183
BspACI CCGC 3 cut(s) 428, 483, 750
BspANI GGCC 2 cut(s) 349, 816
BspCNI CTCAG 3 cut(s) 175, 226, 427
BspLI GGNNCC 5 cut(s) 109, 420, 817, 856, 862
BspPI GGATC 2 cut(s) 391, 856
BsrDI GCAATG 3 cut(s) 228, 581, 635
BsrFI RCCGGY 2 cut(s) 123, 430
BsrI ACTGG 3 cut(s) 297, 548, 895
BssAI RCCGGY 2 cut(s) 123, 430
BssECI CCNNGG 3 cut(s) 439, 794, 841
BssMI GATC 4 cut(s) 46, 383, 589, 848
BssNI GRCGYC 2 cut(s) 529, 875
BssT1I CCWWGG 1 cut(s) 841
Bst2UI CCWGG 1 cut(s) 441
Bst4CI ACNGT 2 cut(s) 42, 211
Bst6I CTCTTC 1 cut(s) 318
BstACI GRCGYC 2 cut(s) 529, 875
BstDEI CTNAG 4 cut(s) 183, 234, 414, 606
BstDSI CCRYGG 1 cut(s) 794
BstEII GGTNACC 1 cut(s) 433
BstF5I GGATG 3 cut(s) 13, 760, 923
BstKTI GATC 4 cut(s) 49, 386, 592, 851
BstMBI GATC 4 cut(s) 46, 383, 589, 848
BstMWI GCNNNNNNNGC 2 cut(s) 396, 427
BstNI CCWGG 1 cut(s) 441
BstNSI RCATGY 1 cut(s) 265
BstPAI GACNNNNGTC 1 cut(s) 893
BstPI GGTNACC 1 cut(s) 433
BstSCI CCNGG 1 cut(s) 439
BstSFI CTRYAG 1 cut(s) 210
BstV2I GAAGAC 3 cut(s) 222, 729, 889
BsuRI GGCC 2 cut(s) 349, 816
BtgI CCRYGG 1 cut(s) 794
BtsCI GGATG 3 cut(s) 13, 760, 923
BtsI GCAGTG 1 cut(s) 397
BtsIMutI CAGTG 1 cut(s) 397
CaiI CAGNNNCTG 1 cut(s) 554
Cfr10I RCCGGY 2 cut(s) 123, 430
Cfr13I GGNCC 2 cut(s) 407, 815
CseI GACGC 2 cut(s) 537, 883
Csp6I GTAC 3 cut(s) 478, 543, 951
CspAI ACCGGT 1 cut(s) 123
CviAII CATG 1 cut(s) 262
CviQI GTAC 3 cut(s) 478, 543, 951
DdeI CTNAG 4 cut(s) 183, 234, 414, 606
DpnI GATC 4 cut(s) 48, 385, 591, 850
DpnII GATC 4 cut(s) 46, 383, 589, 848
EaeI YGGCCR 1 cut(s) 347
Eam1104I CTCTTC 1 cut(s) 318
EarI CTCTTC 1 cut(s) 318
EciI GGCGGA 1 cut(s) 765
Eco130I CCWWGG 1 cut(s) 841
Eco47I GGWCC 1 cut(s) 407
Eco57I CTGAAG 3 cut(s) 159, 198, 576
Eco91I GGTNACC 1 cut(s) 433
EcoO109I RGGNCCY 1 cut(s) 815
EcoO65I GGTNACC 1 cut(s) 433
EcoRII CCWGG 1 cut(s) 439
EcoT14I CCWWGG 1 cut(s) 841
ErhI CCWWGG 1 cut(s) 841
FaeI CATG 1 cut(s) 265
FaiI YATR 4 cut(s) 263, 465, 515, 827
FalI AAGNNNNNCTT 2 cut(s) 171, 203
FaqI GGGAC 3 cut(s) 540, 886, 901
FatI CATG 1 cut(s) 261
FbaI TGATCA 1 cut(s) 589
FblI GTMKAC 2 cut(s) 206, 670
Fnu4HI GCNGC 2 cut(s) 428, 483
FokI GGATG 3 cut(s) 20, 747, 910
Fsp4HI GCNGC 2 cut(s) 428, 483
FspBI CTAG 1 cut(s) 306
GluI GCNGC 2 cut(s) 428, 483
GsuI CTGGAG 1 cut(s) 750
HaeIII GGCC 2 cut(s) 349, 816
HapII CCGG 4 cut(s) 102, 124, 431, 858
HgaI GACGC 2 cut(s) 537, 883
Hin1I GRCGYC 2 cut(s) 529, 875
Hin1II CATG 1 cut(s) 265
HincII GTYRAC 3 cut(s) 16, 207, 240
HindII GTYRAC 3 cut(s) 16, 207, 240
HindIII AAGCTT 1 cut(s) 453
HinfI GANTC 6 cut(s) 203, 241, 253, 326, 356, 602
HpaII CCGG 4 cut(s) 102, 124, 431, 858
HphI GGTGA 3 cut(s) 155, 445, 924
Hpy166II GTNNAC 4 cut(s) 16, 207, 240, 671
Hpy188I TCNGA 5 cut(s) 88, 112, 202, 313, 460
Hpy188III TCNNGA 4 cut(s) 137, 269, 416, 864
Hpy8I GTNNAC 4 cut(s) 16, 207, 240, 671
Hpy99I CGWCG 5 cut(s) 85, 88, 199, 278, 427
HpyAV CCTTC 3 cut(s) 134, 398, 673
HpyCH4III ACNGT 2 cut(s) 42, 211
HpyCH4V TGCA 1 cut(s) 495
HpyF10VI GCNNNNNNNGC 2 cut(s) 396, 427
HpyF3I CTNAG 4 cut(s) 183, 234, 414, 606
Hsp92I GRCGYC 2 cut(s) 529, 875
Hsp92II CATG 1 cut(s) 265
Ksp22I TGATCA 1 cut(s) 589
Kzo9I GATC 4 cut(s) 46, 383, 589, 848
LmnI GCTCC 3 cut(s) 113, 314, 418
LweI GCATC 1 cut(s) 769
MaeI CTAG 1 cut(s) 306
MaeIII GTNAC 4 cut(s) 36, 353, 433, 917
MalI GATC 4 cut(s) 48, 385, 591, 850
MboI GATC 4 cut(s) 46, 383, 589, 848
MboII GAAGA 5 cut(s) 227, 335, 729, 736, 889
MlsI TGGCCA 1 cut(s) 349
MluCI AATT 4 cut(s) 613, 698, 778, 955
MluNI TGGCCA 1 cut(s) 349
MlyI GAGTC 6 cut(s) 212, 235, 247, 335, 350, 596
MmeI TCCRAC 3 cut(s) 46, 66, 480
MnlI CCTC 7 cut(s) 139, 162, 725, 774, 822, 829, 941
Mox20I TGGCCA 1 cut(s) 349
MscI TGGCCA 1 cut(s) 349
MseI TTAA 3 cut(s) 612, 755, 958
Msp20I TGGCCA 1 cut(s) 349
MspI CCGG 4 cut(s) 102, 124, 431, 858
MspR9I CCNGG 1 cut(s) 441
MvaI CCWGG 1 cut(s) 441
MwoI GCNNNNNNNGC 2 cut(s) 396, 427
NdeII GATC 4 cut(s) 46, 383, 589, 848
NlaIII CATG 1 cut(s) 265
NlaIV GGNNCC 5 cut(s) 109, 420, 817, 856, 862
NmuCI GTSAC 2 cut(s) 353, 433
NspI RCATGY 1 cut(s) 265
PcsI WCGNNNNNNNCGW 1 cut(s) 203
Pfl23II CGTACG 1 cut(s) 477
PflFI GACNNNGTC 1 cut(s) 877
PinAI ACCGGT 1 cut(s) 123
PkrI GCNGC 2 cut(s) 429, 484
PleI GAGTC 6 cut(s) 211, 235, 247, 334, 350, 596
PpsI GAGTC 6 cut(s) 211, 235, 247, 334, 350, 596
PshAI GACNNNNGTC 1 cut(s) 893
PshBI ATTAAT 1 cut(s) 612
Psp6I CCWGG 1 cut(s) 439
PspEI GGTNACC 1 cut(s) 433
PspGI CCWGG 1 cut(s) 439
PspLI CGTACG 1 cut(s) 477
PspN4I GGNNCC 5 cut(s) 109, 420, 817, 856, 862
PspPI GGNCC 2 cut(s) 407, 815
PstNI CAGNNNCTG 1 cut(s) 554
PsyI GACNNNGTC 1 cut(s) 877
RsaI GTAC 3 cut(s) 479, 544, 952
RsaNI GTAC 3 cut(s) 478, 543, 951
SalI GTCGAC 1 cut(s) 205
SaqAI TTAA 3 cut(s) 612, 755, 958
SatI GCNGC 2 cut(s) 428, 483
Sau3AI GATC 4 cut(s) 46, 383, 589, 848
Sau96I GGNCC 2 cut(s) 407, 815
SchI GAGTC 6 cut(s) 212, 235, 247, 335, 350, 596
ScrFI CCNGG 1 cut(s) 441
SfaNI GCATC 1 cut(s) 769
SfcI CTRYAG 1 cut(s) 210
SgrAI CRCCGGYG 2 cut(s) 123, 430
SinI GGWCC 1 cut(s) 407
Sse9I AATT 4 cut(s) 613, 698, 778, 955
SsiI CCGC 3 cut(s) 428, 483, 750
SspMI CTAG 1 cut(s) 306
StyD4I CCNGG 1 cut(s) 439
StyI CCWWGG 1 cut(s) 841
TaaI ACNGT 2 cut(s) 42, 211
TaqI TCGA 4 cut(s) 45, 167, 206, 386
TasI AATT 4 cut(s) 613, 698, 778, 955
TauI GCSGC 2 cut(s) 430, 485
Tru1I TTAA 3 cut(s) 612, 755, 958
Tru9I TTAA 3 cut(s) 612, 755, 958
TscAI CASTG 1 cut(s) 397
TseFI GTSAC 2 cut(s) 353, 433
Tsp45I GTSAC 2 cut(s) 353, 433
TspDTI ATGAA 2 cut(s) 107, 530
TspGWI ACGGA 1 cut(s) 69
TspRI CASTG 1 cut(s) 397
Tth111I GACNNNGTC 1 cut(s) 877
VpaK11BI GGWCC 1 cut(s) 407
VspI ATTAAT 1 cut(s) 612
XceI RCATGY 1 cut(s) 265
XmiI GTMKAC 2 cut(s) 206, 670
XspI CTAG 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.