pycom03g06580

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0010345
Physical Location & Seq
Reverse (-)
5169146 .. 5172170
3025 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g06580.5

Sequence Viewer

Length: 1158 bp
ATGATGGATTTTGAGTGCTCCAAACCAGATACCAAGGCCTGGGATATTCGTGAAGCAGCTGCTAACTATAATTACAAAGGATTTATTGGCTCATCAGATTTGAGTTCAACTTCTTCATCTTCAGCACTTGAGGATTCTGAACTTGTTTCATCTGATCAGTGGACAGAGACTACTACTGATGGGGACGACGATTACGTTGCCGAATTAACTCGCCAGATGACTAACTACATGCTTCAAGATGATCACGACAAAACCAAAACTACTAGCTCTCAGAAAAACCAAGAGTTATTGGGTTCTGTTGGTTGGCCACAGTACTCAGTGTGGCCACCGTTGGGGTCTTGCCATGGCAGCTCTGAAGGTTCATCTCTTGAACCAACGCCACCAGAAACACCAGGCAAAGACAAAGCCTGGAACAGGTGTACCTACGCCGACGTTTTAAGAAAGTTGGACAAGTTAGATACAACTACAACCAACGAAGGGTCACGTCATCCTCAGGGAATAAACGCAAGTGCAAAAGTAGACCCAGGTGTTCGATTTTGCTCAAAGCAAGATCTAGCTTCCGGTCAGAGCCAAGATTTTCAGAAACTTGAAGCCTATAATTATAAGCAAAAGCAAGAGCACATTTCAAGGAAACAAGGATCATCAGGGAATAGAGGAAGACAAGCCAATATTTGCCATGAGCAATATGAGCCTAATAATTATCAGCACTTGAAGAAAGGAGGATCTTGTATTGGGTCAATTAATGGAGGAACGTCAAGGTCTCCAACACAATTCGGATTGCCTGGTTCAAACCCATGGGTTAACCAGGAAGCACGACCGGCCGTGAGGGCTGTTTTCCTTGGCGGGTCGAGTTCGAAAATTGGGTCATCTTCTGGGACAGGAGTATTTTTGCCGTGTGTTATTGGTAACACTTCACAGTCACCCAAGAAAAGAGGATGTTCCCCGGTTCTTATACCCGCAAAGGTAGTGCACGCCTTGAAAGTTCACTTTGACAGAGTTGGCGACCTACCTCGACCTAATGTTTCTGGTTTCTCTACACAAAATGATGATTTGAAGGGTGGTAGGGCAGACATGCATTCAACTAATAAGCATCAGTCGCGGCCGGTGCCATGCATGAACCATAAGGAGATGGGTCTGCCTCAAGAATGGACATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

386

Amino Acids

42.13

Weight (kDa)

7.15

Isoelectric Point (pI)

51.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010345)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G59050 AT5G59050 AT5G59050 AT5G59050
fragaria_vesca FvH4_3g37230 FvH4_3g37230
malus_domestica MD03G1082700.v1.1 MD11G1090800.v1.1
prunus_persica Prupe.6G067000_v2.0.a1
pyrus_communis pycom03g06580 pycom11g07550
rosa_chinensis RchiOBHm_Chr5g0066981
rosa_laevigata RLG00000035898
rosa_multiflora Rmu_sc0008378.1_g000002
rosa_roxburghii Rroxscaffold_1G00014150
rosa_rugosa Rorug05G0380300
rosa_samantha Rh5AG439900 Rh5BG457000 Rh5CG479100 Rh5DG471700
rosa_wichuraiana Rw5G041140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 603
AccB1I GGYRCC 1 cut(s) 1105
AccB7I CCANNNNNTGG 1 cut(s) 39
AccI GTMKAC 1 cut(s) 519
AccII CGCG 1 cut(s) 1099
AciI CCGC 3 cut(s) 843, 957, 1099
AclWI GGATC 2 cut(s) 646, 730
AcoI YGGCCR 4 cut(s) 305, 323, 819, 1100
AcuI CTGAAG 2 cut(s) 105, 375
AfaI GTAC 2 cut(s) 314, 421
AfiI CCNNNNNNNGG 4 cut(s) 39, 332, 414, 477
AjiI CACGTC 1 cut(s) 485
AjnI CCWGG 6 cut(s) 38, 391, 407, 523, 781, 804
AluBI AGCT 4 cut(s) 59, 267, 351, 557
AluI AGCT 4 cut(s) 59, 267, 351, 557
Alw21I GWGCWC 3 cut(s) 20, 621, 972
Alw26I GTCTC 2 cut(s) 161, 765
Alw44I GTGCAC 1 cut(s) 968
AlwI GGATC 2 cut(s) 646, 730
AoxI GGCC 5 cut(s) 36, 305, 323, 819, 1100
ApaLI GTGCAC 1 cut(s) 968
ApeKI GCWGC 3 cut(s) 56, 59, 348
ArsI GACNNNNNNTTYG 2 cut(s) 848, 880
AseI ATTAAT 1 cut(s) 741
AsuC2I CCSGG 1 cut(s) 944
AsuHPI GGTGA 1 cut(s) 912
AsuII TTCGAA 1 cut(s) 854
AxyI CCTNAGG 1 cut(s) 492
BaeGI GKGCMC 1 cut(s) 972
BaeI ACNNNNGTAYC 2 cut(s) 403, 436
BalI TGGCCA 2 cut(s) 307, 325
BanI GGYRCC 1 cut(s) 1105
BbsI GAAGAC 1 cut(s) 664
Bbv12I GWGCWC 3 cut(s) 20, 621, 972
BbvI GCAGC 3 cut(s) 46, 68, 360
BccI CCATC 2 cut(s) 173, 1123
BceAI ACGGC 2 cut(s) 806, 877
BcgI CGANNNNNNTGC 2 cut(s) 179, 213
BciT130I CCWGG 6 cut(s) 40, 393, 409, 525, 783, 806
BclI TGATCA 2 cut(s) 154, 241
BcnI CCSGG 1 cut(s) 944
BcoDI GTCTC 2 cut(s) 161, 765
BfaI CTAG 2 cut(s) 264, 554
BglI GCCNNNNNGGC 1 cut(s) 827
BglII AGATCT 1 cut(s) 550
BisI GCNGC 4 cut(s) 57, 60, 349, 1100
BlsI GCNGC 4 cut(s) 58, 61, 350, 1101
BmcAI AGTACT 1 cut(s) 314
Bme1390I CCNGG 7 cut(s) 40, 393, 409, 525, 783, 806, 944
BmgBI CACGTC 1 cut(s) 485
BmiI GGNNCC 1 cut(s) 1107
BmrFI CCNGG 7 cut(s) 40, 393, 409, 525, 783, 806, 944
BmsI GCATC 1 cut(s) 1099
BpiI GAAGAC 1 cut(s) 664
Bpu14I TTCGAA 1 cut(s) 854
BpuEI CTTGAG 2 cut(s) 149, 1125
BpuMI CCSGG 1 cut(s) 944
BsaI GGTCTC 1 cut(s) 765
BsaJI CCNNGG 7 cut(s) 33, 39, 343, 523, 794, 838, 942
BsaWI WCCGGW 1 cut(s) 560
Bsc4I CCNNNNNNNGG 4 cut(s) 39, 332, 414, 477
Bse118I RCCGGY 2 cut(s) 817, 1102
Bse21I CCTNAGG 1 cut(s) 492
BseBI CCWGG 6 cut(s) 40, 393, 409, 525, 783, 806
BseDI CCNNGG 7 cut(s) 33, 39, 343, 523, 794, 838, 942
BseGI GGATG 2 cut(s) 487, 941
BseLI CCNNNNNNNGG 4 cut(s) 39, 332, 414, 477
BseMII CTCAG 3 cut(s) 284, 330, 506
BseSI GKGCMC 1 cut(s) 972
BseX3I CGGCCG 2 cut(s) 819, 1100
BseXI GCAGC 3 cut(s) 46, 68, 360
Bsh1236I CGCG 1 cut(s) 1099
Bsh1285I CGRYCG 3 cut(s) 818, 822, 1103
BshFI GGCC 5 cut(s) 38, 307, 325, 821, 1102
BshNI GGYRCC 1 cut(s) 1105
BsiEI CGRYCG 3 cut(s) 818, 822, 1103
BsiHKAI GWGCWC 3 cut(s) 20, 621, 972
BsiSI CCGG 4 cut(s) 561, 818, 944, 1103
BslFI GGGAC 2 cut(s) 197, 889
BslI CCNNNNNNNGG 4 cut(s) 39, 332, 414, 477
BsmAI GTCTC 2 cut(s) 161, 765
BsmFI GGGAC 2 cut(s) 197, 889
BsmI GAATGC 1 cut(s) 1075
BsnI GGCC 5 cut(s) 38, 307, 325, 821, 1102
Bso31I GGTCTC 1 cut(s) 765
Bsp119I TTCGAA 1 cut(s) 854
Bsp1286I GDGCHC 3 cut(s) 20, 621, 972
Bsp143I GATC 5 cut(s) 154, 241, 550, 638, 722
Bsp19I CCATGG 2 cut(s) 343, 794
BspACI CCGC 3 cut(s) 843, 957, 1099
BspANI GGCC 5 cut(s) 38, 307, 325, 821, 1102
BspCNI CTCAG 3 cut(s) 283, 329, 505
BspFNI CGCG 1 cut(s) 1099
BspLI GGNNCC 1 cut(s) 1107
BspPI GGATC 2 cut(s) 646, 730
BspT104I TTCGAA 1 cut(s) 854
BspT107I GGYRCC 1 cut(s) 1105
BspTNI GGTCTC 1 cut(s) 765
BsrFI RCCGGY 2 cut(s) 817, 1102
BssAI RCCGGY 2 cut(s) 817, 1102
BssECI CCNNGG 7 cut(s) 33, 39, 343, 523, 794, 838, 942
BssMI GATC 5 cut(s) 154, 241, 550, 638, 722
BssT1I CCWWGG 4 cut(s) 33, 343, 794, 838
Bst2UI CCWGG 6 cut(s) 40, 393, 409, 525, 783, 806
Bst4CI ACNGT 3 cut(s) 312, 330, 918
BstBI TTCGAA 1 cut(s) 854
BstC8I GCNNGC 1 cut(s) 972
BstDEI CTNAG 3 cut(s) 270, 316, 492
BstDSI CCRYGG 2 cut(s) 343, 794
BstENI CCTNNNNNAGG 1 cut(s) 412
BstF5I GGATG 2 cut(s) 487, 941
BstFNI CGCG 1 cut(s) 1099
BstKTI GATC 5 cut(s) 157, 244, 553, 641, 725
BstMAI GTCTC 2 cut(s) 161, 765
BstMBI GATC 5 cut(s) 154, 241, 550, 638, 722
BstMCI CGRYCG 3 cut(s) 818, 822, 1103
BstMWI GCNNNNNNNGC 6 cut(s) 348, 688, 818, 827, 1096, 1105
BstNI CCWGG 6 cut(s) 40, 393, 409, 525, 783, 806
BstNSI RCATGY 2 cut(s) 232, 1075
BstSCI CCNGG 7 cut(s) 38, 391, 407, 523, 781, 804, 942
BstSLI GKGCMC 1 cut(s) 972
BstUI CGCG 1 cut(s) 1099
BstV1I GCAGC 3 cut(s) 46, 68, 360
BstV2I GAAGAC 1 cut(s) 664
BstX2I RGATCY 2 cut(s) 550, 722
BstYI RGATCY 2 cut(s) 550, 722
BstZI CGGCCG 2 cut(s) 819, 1100
Bsu36I CCTNAGG 1 cut(s) 492
BsuRI GGCC 5 cut(s) 38, 307, 325, 821, 1102
BtgI CCRYGG 2 cut(s) 343, 794
BtrI CACGTC 1 cut(s) 485
BtsCI GGATG 2 cut(s) 487, 941
BtsIMutI CAGTG 2 cut(s) 164, 324
Cac8I GCNNGC 1 cut(s) 972
Cfr10I RCCGGY 2 cut(s) 817, 1102
Csp6I GTAC 2 cut(s) 313, 420
CviAII CATG 7 cut(s) 229, 344, 677, 795, 1072, 1110, 1114
CviQI GTAC 2 cut(s) 313, 420
DdeI CTNAG 3 cut(s) 270, 316, 492
DpnI GATC 5 cut(s) 156, 243, 552, 640, 724
DpnII GATC 5 cut(s) 154, 241, 550, 638, 722
EaeI YGGCCR 4 cut(s) 305, 323, 819, 1100
EagI CGGCCG 2 cut(s) 819, 1100
EclXI CGGCCG 2 cut(s) 819, 1100
Eco130I CCWWGG 4 cut(s) 33, 343, 794, 838
Eco147I AGGCCT 1 cut(s) 38
Eco31I GGTCTC 1 cut(s) 765
Eco52I CGGCCG 2 cut(s) 819, 1100
Eco57I CTGAAG 2 cut(s) 105, 375
Eco81I CCTNAGG 1 cut(s) 492
EcoNI CCTNNNNNAGG 1 cut(s) 412
EcoRII CCWGG 6 cut(s) 38, 391, 407, 523, 781, 804
EcoT14I CCWWGG 4 cut(s) 33, 343, 794, 838
EcoT22I ATGCAT 2 cut(s) 1077, 1115
ErhI CCWWGG 4 cut(s) 33, 343, 794, 838
FaeI CATG 7 cut(s) 232, 347, 680, 798, 1075, 1113, 1117
FaqI GGGAC 2 cut(s) 197, 889
FatI CATG 7 cut(s) 228, 343, 676, 794, 1071, 1109, 1113
FauI CCCGC 2 cut(s) 836, 964
FbaI TGATCA 2 cut(s) 154, 241
FblI GTMKAC 1 cut(s) 519
Fnu4HI GCNGC 4 cut(s) 57, 60, 349, 1100
FokI GGATG 2 cut(s) 474, 948
Fsp4HI GCNGC 4 cut(s) 57, 60, 349, 1100
FspBI CTAG 2 cut(s) 264, 554
GluI GCNGC 4 cut(s) 57, 60, 349, 1100
HaeIII GGCC 5 cut(s) 38, 307, 325, 821, 1102
HapII CCGG 4 cut(s) 561, 818, 944, 1103
Hin1II CATG 7 cut(s) 232, 347, 680, 798, 1075, 1113, 1117
HincII GTYRAC 1 cut(s) 802
HindII GTYRAC 1 cut(s) 802
HinfI GANTC 1 cut(s) 134
HpaI GTTAAC 1 cut(s) 802
HpaII CCGG 4 cut(s) 561, 818, 944, 1103
HphI GGTGA 1 cut(s) 912
Hpy166II GTNNAC 6 cut(s) 162, 420, 520, 802, 970, 985
Hpy188I TCNGA 8 cut(s) 97, 139, 154, 273, 355, 567, 582, 776
Hpy188III TCNNGA 5 cut(s) 50, 236, 245, 368, 1142
Hpy8I GTNNAC 6 cut(s) 162, 420, 520, 802, 970, 985
Hpy99I CGWCG 2 cut(s) 191, 434
HpyAV CCTTC 3 cut(s) 350, 470, 1048
HpyCH4III ACNGT 3 cut(s) 312, 330, 918
HpyCH4IV ACGT 4 cut(s) 195, 432, 484, 752
HpyCH4V TGCA 4 cut(s) 512, 970, 1075, 1113
HpyF10VI GCNNNNNNNGC 6 cut(s) 348, 688, 818, 827, 1096, 1105
HpyF3I CTNAG 3 cut(s) 270, 316, 492
HpySE526I ACGT 4 cut(s) 195, 432, 484, 752
Hsp92II CATG 7 cut(s) 232, 347, 680, 798, 1075, 1113, 1117
Ksp22I TGATCA 2 cut(s) 154, 241
KspAI GTTAAC 1 cut(s) 802
Kzo9I GATC 5 cut(s) 154, 241, 550, 638, 722
LmnI GCTCC 1 cut(s) 23
Lsp1109I GCAGC 3 cut(s) 46, 68, 360
LweI GCATC 1 cut(s) 1099
MaeI CTAG 2 cut(s) 264, 554
MaeII ACGT 4 cut(s) 195, 432, 484, 752
MaeIII GTNAC 3 cut(s) 480, 905, 918
MalI GATC 5 cut(s) 156, 243, 552, 640, 724
MboI GATC 5 cut(s) 154, 241, 550, 638, 722
MboII GAAGA 5 cut(s) 105, 111, 669, 724, 861
MflI RGATCY 2 cut(s) 550, 722
MhlI GDGCHC 3 cut(s) 20, 621, 972
MlsI TGGCCA 2 cut(s) 307, 325
MluCI AATT 7 cut(s) 70, 203, 598, 697, 738, 770, 858
MluNI TGGCCA 2 cut(s) 307, 325
MmeI TCCRAC 2 cut(s) 426, 788
MnlI CCTC 9 cut(s) 124, 501, 647, 713, 740, 819, 926, 1020, 1149
Mox20I TGGCCA 2 cut(s) 307, 325
Mph1103I ATGCAT 2 cut(s) 1077, 1115
MscI TGGCCA 2 cut(s) 307, 325
MseI TTAA 4 cut(s) 206, 437, 741, 801
Msp20I TGGCCA 2 cut(s) 307, 325
MspA1I CMGCKG 1 cut(s) 59
MspI CCGG 4 cut(s) 561, 818, 944, 1103
MspR9I CCNGG 7 cut(s) 40, 393, 409, 525, 783, 806, 944
Mva1269I GAATGC 1 cut(s) 1075
MvaI CCWGG 6 cut(s) 40, 393, 409, 525, 783, 806
MvnI CGCG 1 cut(s) 1099
MwoI GCNNNNNNNGC 6 cut(s) 348, 688, 818, 827, 1096, 1105
NciI CCSGG 1 cut(s) 944
NcoI CCATGG 2 cut(s) 343, 794
NdeII GATC 5 cut(s) 154, 241, 550, 638, 722
NlaIII CATG 7 cut(s) 232, 347, 680, 798, 1075, 1113, 1117
NlaIV GGNNCC 1 cut(s) 1107
NmuCI GTSAC 2 cut(s) 480, 918
NsiI ATGCAT 2 cut(s) 1077, 1115
NspI RCATGY 2 cut(s) 232, 1075
NspV TTCGAA 1 cut(s) 854
PceI AGGCCT 1 cut(s) 38
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PctI GAATGC 1 cut(s) 1075
PfeI GAWTC 1 cut(s) 134
PflMI CCANNNNNTGG 1 cut(s) 39
PkrI GCNGC 4 cut(s) 58, 61, 350, 1101
PshBI ATTAAT 1 cut(s) 741
PsiI TTATAA 1 cut(s) 603
Psp6I CCWGG 6 cut(s) 38, 391, 407, 523, 781, 804
PspGI CCWGG 6 cut(s) 38, 391, 407, 523, 781, 804
PspN4I GGNNCC 1 cut(s) 1107
PsuI RGATCY 2 cut(s) 550, 722
PvuII CAGCTG 1 cut(s) 59
RsaI GTAC 2 cut(s) 314, 421
RsaNI GTAC 2 cut(s) 313, 420
SaqAI TTAA 4 cut(s) 206, 437, 741, 801
SatI GCNGC 4 cut(s) 57, 60, 349, 1100
Sau3AI GATC 5 cut(s) 154, 241, 550, 638, 722
ScaI AGTACT 1 cut(s) 314
ScrFI CCNGG 7 cut(s) 40, 393, 409, 525, 783, 806, 944
SduI GDGCHC 3 cut(s) 20, 621, 972
SfaNI GCATC 1 cut(s) 1099
SfuI TTCGAA 1 cut(s) 854
SmlI CTYRAG 2 cut(s) 128, 1140
SmoI CTYRAG 2 cut(s) 128, 1140
Sse9I AATT 7 cut(s) 70, 203, 598, 697, 738, 770, 858
SseBI AGGCCT 1 cut(s) 38
SsiI CCGC 3 cut(s) 843, 957, 1099
SspI AATATT 1 cut(s) 670
SspMI CTAG 2 cut(s) 264, 554
StuI AGGCCT 1 cut(s) 38
StyD4I CCNGG 7 cut(s) 38, 391, 407, 523, 781, 804, 942
StyI CCWWGG 4 cut(s) 33, 343, 794, 838
TaaI ACNGT 3 cut(s) 312, 330, 918
TaiI ACGT 4 cut(s) 198, 435, 487, 755
TaqI TCGA 4 cut(s) 532, 848, 854, 1012
TasI AATT 7 cut(s) 70, 203, 598, 697, 738, 770, 858
TatI WGTACW 1 cut(s) 312
TauI GCSGC 1 cut(s) 1102
TfiI GAWTC 1 cut(s) 134
Tru1I TTAA 4 cut(s) 206, 437, 741, 801
Tru9I TTAA 4 cut(s) 206, 437, 741, 801
TscAI CASTG 2 cut(s) 164, 324
TseFI GTSAC 2 cut(s) 480, 918
TseI GCWGC 3 cut(s) 56, 59, 348
Tsp45I GTSAC 2 cut(s) 480, 918
TspDTI ATGAA 4 cut(s) 105, 138, 351, 1130
TspRI CASTG 2 cut(s) 164, 324
Van91I CCANNNNNTGG 1 cut(s) 39
VneI GTGCAC 1 cut(s) 968
VspI ATTAAT 1 cut(s) 741
XagI CCTNNNNNAGG 1 cut(s) 412
XceI RCATGY 2 cut(s) 232, 1075
XmiI GTMKAC 1 cut(s) 519
XspI CTAG 2 cut(s) 264, 554
ZrmI AGTACT 1 cut(s) 314
Zsp2I ATGCAT 2 cut(s) 1077, 1115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.