MD03G1083200.v1.1

Pentatricopeptide repeat-containing protein At3g46870-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0006716
Physical Location & Seq
Reverse (-)
6738230 .. 6740369
2140 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1083200.v1.1.491

Sequence Viewer

Length: 738 bp
ATGGCGACTCGCGCATTTTCGCGATCAAAGTCGTCATTTTTCTCCTCAGTACTCCTTCAAAACCTAAAAACAACACCAATCAAAACCACCCCATTGCTTCTAAACTCCACAATTCCAGAGCTCGAAATCAAATCCTGCAGACCCATTTCTGGGTTCAGGCTGTATCACGACGGGAGGCCCAGAGGACCGCTCTGGAGGGGCAAGAAGCTGATTGGCAAAGAAGCGCTTTTCGTAATCTCGGGTTTGAAGCGGTTCAAGGACAACGAAGAGAAGCTCGGGAAGTTTGTAAAGACCCACGTTTTGAGGCTTCTGAAGATGGATATGATTGCTGTGCTCACGGAGCTCGAGCGCCAGGAAGAAGTTGCTTTGGCAATTAAGGTATTTAACGTGATTCGAAGGCAAGACTGGTACAAACCGGATGTCTATCTGTACAAGGACTTGATTATTGCATTGGCAAGATCTAAGAAAATGGAGGATGTTATGCTACTGTGGGACATCATGAAAAAGGAGGATCTATTTCCTGATTCTCAAACATATACAGAAGTTATCAGGGGTTTTTTGAACTCTGGTTGTCCAGCAGATGCGATGAACATATATGAGGACATGAAGCAGTCTCCAGATCCACCGGAGGAGCTGCCGTTCCGGATTTTGTTGAAGGGACTTCTGCCACATCCCCTTTTGAGAAACAGAGTCAAGCAAGACTTTGAGGAGCTGTTTCCCGAACAGCATGTGGCTTAA

Protein Analysis

246

Amino Acids

28.48

Weight (kDa)

9.38

Isoelectric Point (pI)

54.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 139 - 187 2.3e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 190
AccII CGCG 2 cut(s) 12, 22
AccIII TCCGGA 1 cut(s) 642
AciI CCGC 2 cut(s) 188, 250
AclWI GGATC 2 cut(s) 519, 614
AcuI CTGAAG 1 cut(s) 332
AfaI GTAC 3 cut(s) 51, 410, 431
AfeI AGCGCT 1 cut(s) 225
AfiI CCNNNNNNNGG 2 cut(s) 149, 150
AgsI TTSAA 5 cut(s) 59, 247, 256, 562, 655
AjnI CCWGG 1 cut(s) 351
AloI GAACNNNNNNTCC 2 cut(s) 623, 655
AluBI AGCT 6 cut(s) 121, 208, 274, 343, 634, 712
AluI AGCT 6 cut(s) 121, 208, 274, 343, 634, 712
Alw21I GWGCWC 3 cut(s) 123, 336, 345
Alw26I GTCTC 1 cut(s) 618
AlwI GGATC 2 cut(s) 519, 614
Ama87I CYCGRG 3 cut(s) 238, 275, 344
Aor13HI TCCGGA 1 cut(s) 642
Aor51HI AGCGCT 1 cut(s) 225
AoxI GGCC 1 cut(s) 176
ApeKI GCWGC 1 cut(s) 634
ArsI GACNNNNNNTTYG 4 cut(s) 283, 315, 405, 437
Asp700I GAANNNNTTC 1 cut(s) 251
AspLEI GCGC 3 cut(s) 14, 226, 351
AspS9I GGNCC 2 cut(s) 177, 185
AsuII TTCGAA 1 cut(s) 394
AvaI CYCGRG 3 cut(s) 238, 275, 344
AvaII GGWCC 1 cut(s) 185
BanII GRGCYC 2 cut(s) 123, 345
Bbv12I GWGCWC 3 cut(s) 123, 336, 345
BbvI GCAGC 1 cut(s) 621
BccI CCATC 1 cut(s) 310
BceAI ACGGC 1 cut(s) 622
BciT130I CCWGG 1 cut(s) 353
BcoDI GTCTC 1 cut(s) 618
BfmI CTRYAG 1 cut(s) 136
BfoI RGCGCY 2 cut(s) 227, 352
BglII AGATCT 1 cut(s) 458
BisI GCNGC 1 cut(s) 635
BlsI GCNGC 1 cut(s) 636
BmcAI AGTACT 1 cut(s) 51
Bme1390I CCNGG 1 cut(s) 353
Bme18I GGWCC 1 cut(s) 185
BmeT110I CYCGRG 3 cut(s) 238, 275, 344
BmgT120I GGNCC 2 cut(s) 177, 185
BmrFI CCNGG 1 cut(s) 353
BmsI GCATC 1 cut(s) 571
BplI GAGNNNNNCTC 2 cut(s) 174, 206
BpmI CTGGAG 2 cut(s) 214, 600
Bpu14I TTCGAA 1 cut(s) 394
BsaBI GATNNNNATC 1 cut(s) 423
BsaWI WCCGGW 3 cut(s) 415, 625, 642
BsaXI ACNNNNNCTCC 2 cut(s) 623, 653
Bsc4I CCNNNNNNNGG 2 cut(s) 149, 150
Bse1I ACTGG 1 cut(s) 410
Bse3DI GCAATG 1 cut(s) 92
Bse8I GATNNNNATC 1 cut(s) 423
BseAI TCCGGA 1 cut(s) 642
BseBI CCWGG 1 cut(s) 353
BseGI GGATG 3 cut(s) 424, 481, 670
BseJI GATNNNNATC 1 cut(s) 423
BseLI CCNNNNNNNGG 2 cut(s) 149, 150
BseMI GCAATG 1 cut(s) 92
BseMII CTCAG 1 cut(s) 60
BseNI ACTGG 1 cut(s) 410
BseRI GAGGAG 3 cut(s) 34, 644, 722
BseXI GCAGC 1 cut(s) 621
Bsh1236I CGCG 2 cut(s) 12, 22
BshFI GGCC 1 cut(s) 178
BsiHKAI GWGCWC 3 cut(s) 123, 336, 345
BsiHKCI CYCGRG 3 cut(s) 238, 275, 344
BsiSI CCGG 3 cut(s) 416, 626, 643
BslFI GGGAC 2 cut(s) 506, 672
BslI CCNNNNNNNGG 2 cut(s) 149, 150
BsmAI GTCTC 1 cut(s) 618
BsmFI GGGAC 2 cut(s) 506, 672
BsnI GGCC 1 cut(s) 178
BsoBI CYCGRG 3 cut(s) 238, 275, 344
Bsp119I TTCGAA 1 cut(s) 394
Bsp1286I GDGCHC 3 cut(s) 123, 336, 345
Bsp13I TCCGGA 1 cut(s) 642
Bsp1407I TGTACA 1 cut(s) 429
Bsp143I GATC 4 cut(s) 23, 458, 511, 619
Bsp68I TCGCGA 1 cut(s) 22
BspACI CCGC 2 cut(s) 188, 250
BspANI GGCC 1 cut(s) 178
BspCNI CTCAG 1 cut(s) 59
BspEI TCCGGA 1 cut(s) 642
BspFNI CGCG 2 cut(s) 12, 22
BspHI TCATGA 1 cut(s) 498
BspMAI CTGCAG 1 cut(s) 140
BspPI GGATC 2 cut(s) 519, 614
BspT104I TTCGAA 1 cut(s) 394
BsrBI CCGCTC 1 cut(s) 190
BsrDI GCAATG 1 cut(s) 92
BsrGI TGTACA 1 cut(s) 429
BsrI ACTGG 1 cut(s) 410
BssMI GATC 4 cut(s) 23, 458, 511, 619
Bst2UI CCWGG 1 cut(s) 353
Bst4CI ACNGT 1 cut(s) 489
Bst6I CTCTTC 1 cut(s) 261
BstAUI TGTACA 1 cut(s) 429
BstBI TTCGAA 1 cut(s) 394
BstDEI CTNAG 2 cut(s) 46, 462
BstF5I GGATG 3 cut(s) 424, 481, 670
BstFNI CGCG 2 cut(s) 12, 22
BstH2I RGCGCY 2 cut(s) 227, 352
BstHHI GCGC 3 cut(s) 14, 226, 351
BstKTI GATC 4 cut(s) 26, 461, 514, 622
BstMAI GTCTC 1 cut(s) 618
BstMBI GATC 4 cut(s) 23, 458, 511, 619
BstMWI GCNNNNNNNGC 2 cut(s) 11, 340
BstNI CCWGG 1 cut(s) 353
BstNSI RCATGY 1 cut(s) 731
BstSCI CCNGG 1 cut(s) 351
BstSFI CTRYAG 1 cut(s) 136
BstUI CGCG 2 cut(s) 12, 22
BstV1I GCAGC 1 cut(s) 621
BstX2I RGATCY 3 cut(s) 458, 511, 619
BstYI RGATCY 3 cut(s) 458, 511, 619
BsuRI GGCC 1 cut(s) 178
BtgZI GCGATG 1 cut(s) 599
BtsCI GGATG 3 cut(s) 424, 481, 670
BtuMI TCGCGA 1 cut(s) 22
CciI TCATGA 1 cut(s) 498
CfoI GCGC 3 cut(s) 14, 226, 351
Cfr13I GGNCC 2 cut(s) 177, 185
Csp6I GTAC 3 cut(s) 50, 409, 430
CviAII CATG 3 cut(s) 499, 604, 728
CviQI GTAC 3 cut(s) 50, 409, 430
DdeI CTNAG 2 cut(s) 46, 462
DpnI GATC 4 cut(s) 25, 460, 513, 621
DpnII GATC 4 cut(s) 23, 458, 511, 619
Eam1104I CTCTTC 1 cut(s) 261
EarI CTCTTC 1 cut(s) 261
Ecl136II GAGCTC 2 cut(s) 121, 343
Eco24I GRGCYC 2 cut(s) 123, 345
Eco47I GGWCC 1 cut(s) 185
Eco47III AGCGCT 1 cut(s) 225
Eco53kI GAGCTC 2 cut(s) 121, 343
Eco57I CTGAAG 1 cut(s) 332
Eco88I CYCGRG 3 cut(s) 238, 275, 344
EcoICRI GAGCTC 2 cut(s) 121, 343
EcoRII CCWGG 1 cut(s) 351
EcoT38I GRGCYC 2 cut(s) 123, 345
FaeI CATG 3 cut(s) 502, 607, 731
FalI AAGNNNNNCTT 4 cut(s) 210, 242, 686, 718
FaqI GGGAC 2 cut(s) 506, 672
FatI CATG 3 cut(s) 498, 603, 727
Fnu4HI GCNGC 1 cut(s) 635
FokI GGATG 3 cut(s) 431, 488, 657
FriOI GRGCYC 2 cut(s) 123, 345
Fsp4HI GCNGC 1 cut(s) 635
GlaI GCGC 3 cut(s) 13, 225, 350
GluI GCNGC 1 cut(s) 635
GsuI CTGGAG 2 cut(s) 214, 600
HaeII RGCGCY 2 cut(s) 227, 352
HaeIII GGCC 1 cut(s) 178
HapII CCGG 3 cut(s) 416, 626, 643
HhaI GCGC 3 cut(s) 14, 226, 351
Hin1II CATG 3 cut(s) 502, 607, 731
Hin6I GCGC 3 cut(s) 12, 224, 349
HinP1I GCGC 3 cut(s) 12, 224, 349
HinfI GANTC 4 cut(s) 7, 391, 524, 690
HpaII CCGG 3 cut(s) 416, 626, 643
Hpy188I TCNGA 1 cut(s) 312
Hpy99I CGWCG 1 cut(s) 173
HpyAV CCTTC 3 cut(s) 65, 390, 649
HpyCH4III ACNGT 1 cut(s) 489
HpyCH4IV ACGT 2 cut(s) 297, 387
HpyCH4V TGCA 2 cut(s) 138, 449
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 340
HpyF3I CTNAG 2 cut(s) 46, 462
HpySE526I ACGT 2 cut(s) 297, 387
Hsp92II CATG 3 cut(s) 502, 607, 731
HspAI GCGC 3 cut(s) 12, 224, 349
Kpn2I TCCGGA 1 cut(s) 642
Kzo9I GATC 4 cut(s) 23, 458, 511, 619
LmnI GCTCC 3 cut(s) 340, 631, 709
Lsp1109I GCAGC 1 cut(s) 621
LweI GCATC 1 cut(s) 571
MaeII ACGT 2 cut(s) 297, 387
MalI GATC 4 cut(s) 25, 460, 513, 621
MbiI CCGCTC 1 cut(s) 190
MboI GATC 4 cut(s) 23, 458, 511, 619
MboII GAAGA 3 cut(s) 278, 325, 368
MflI RGATCY 3 cut(s) 458, 511, 619
MhlI GDGCHC 3 cut(s) 123, 336, 345
MluCI AATT 2 cut(s) 111, 372
MlyI GAGTC 1 cut(s) 699
MroI TCCGGA 1 cut(s) 642
MroXI GAANNNNTTC 1 cut(s) 251
MseI TTAA 3 cut(s) 375, 384, 736
MspI CCGG 3 cut(s) 416, 626, 643
MspR9I CCNGG 1 cut(s) 353
MvaI CCWGG 1 cut(s) 353
MvnI CGCG 2 cut(s) 12, 22
MwoI GCNNNNNNNGC 2 cut(s) 11, 340
NdeII GATC 4 cut(s) 23, 458, 511, 619
NlaIII CATG 3 cut(s) 502, 607, 731
NruI TCGCGA 1 cut(s) 22
NspI RCATGY 1 cut(s) 731
NspV TTCGAA 1 cut(s) 394
PaeR7I CTCGAG 1 cut(s) 344
PagI TCATGA 1 cut(s) 498
PdmI GAANNNNTTC 1 cut(s) 251
PfeI GAWTC 2 cut(s) 391, 524
PkrI GCNGC 1 cut(s) 636
PleI GAGTC 1 cut(s) 698
PpsI GAGTC 1 cut(s) 698
Psp124BI GAGCTC 2 cut(s) 123, 345
Psp6I CCWGG 1 cut(s) 351
PspGI CCWGG 1 cut(s) 351
PspPI GGNCC 2 cut(s) 177, 185
PspXI VCTCGAGB 1 cut(s) 344
PstI CTGCAG 1 cut(s) 140
PsuI RGATCY 3 cut(s) 458, 511, 619
RruI TCGCGA 1 cut(s) 22
RsaI GTAC 3 cut(s) 51, 410, 431
RsaNI GTAC 3 cut(s) 50, 409, 430
SacI GAGCTC 2 cut(s) 123, 345
SaqAI TTAA 3 cut(s) 375, 384, 736
SatI GCNGC 1 cut(s) 635
Sau3AI GATC 4 cut(s) 23, 458, 511, 619
Sau96I GGNCC 2 cut(s) 177, 185
ScaI AGTACT 1 cut(s) 51
SchI GAGTC 1 cut(s) 699
ScrFI CCNGG 1 cut(s) 353
SduI GDGCHC 3 cut(s) 123, 336, 345
SfaNI GCATC 1 cut(s) 571
SfcI CTRYAG 1 cut(s) 136
Sfr274I CTCGAG 1 cut(s) 344
SfuI TTCGAA 1 cut(s) 394
SinI GGWCC 1 cut(s) 185
SlaI CTCGAG 1 cut(s) 344
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
Sse9I AATT 2 cut(s) 111, 372
SsiI CCGC 2 cut(s) 188, 250
SstI GAGCTC 2 cut(s) 123, 345
StyD4I CCNGG 1 cut(s) 351
TaaI ACNGT 1 cut(s) 489
TaiI ACGT 2 cut(s) 300, 390
TaqI TCGA 3 cut(s) 123, 345, 394
TasI AATT 2 cut(s) 111, 372
TatI WGTACW 2 cut(s) 49, 429
TfiI GAWTC 2 cut(s) 391, 524
Tru1I TTAA 3 cut(s) 375, 384, 736
Tru9I TTAA 3 cut(s) 375, 384, 736
TseI GCWGC 1 cut(s) 634
TspDTI ATGAA 3 cut(s) 515, 602, 620
TspGWI ACGGA 1 cut(s) 353
VpaK11BI GGWCC 1 cut(s) 185
XceI RCATGY 1 cut(s) 731
XhoI CTCGAG 1 cut(s) 344
XmnI GAANNNNTTC 1 cut(s) 251
ZrmI AGTACT 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.