Rh7CG410600

pentatricopeptide repeat-containing protein At1g31840

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0006716
Physical Location & Seq
Forward (+)
52680524 .. 52680964
441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG410600.1

Sequence Viewer

Length: 441 bp
ATGGAAGATGCAGTGTTATTGTTTGATCAAATGCTGGAAGAGGATCCTGAACCTAATGTGGTCACCTATAGTTGCCTGATTGATGGTTATTTTAAATCTGAAAGCATGGAAAGTGCCTTTGGACTATATGAAGAGATGCTTCAGAGTATCTCTCCAAATATAGTGAGTTACAGCATCCTCATTGATGGTCTTTGCAAAAGAGGACTGATGGAGAAAGCAGCCCTTGCATTTCATTCTGCTATAGACAGGGGTCTGGTGCCGGATGTAGTAGCATATGGGATTCTGATTCATGGTTACTGCAGGGTTGGAAGAACGGCAGAAGCCATGGTATTGTACGGTCGTATGTTACTAAGTGGAATAATGCCAGATGCTATCTTACAAAGGATGATTGCAAAGCACCTTCTTGAAATCCGGTTCCACCAATCAGATGATGTTCTTTAA

Protein Analysis

146

Amino Acids

16.35

Weight (kDa)

4.67

Isoelectric Point (pI)

46.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 1 - 32 2.9e-07 PPR repeat family
PPR_3 PF13812 7 - 62 1.2e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 17 - 46 6.3e-09 PPR repeat
PPR_2 PF13041 18 - 66 1.5e-17 PPR repeat family
PPR PF01535 21 - 48 9.2e-07 PPR repeat
PPR_1 PF12854 50 - 73 8.1e-09 PPR repeat
PPR_2 PF13041 52 - 97 1.5e-10 PPR repeat family
PPR_1 PF12854 83 - 115 3.7e-11 PPR repeat
PPR_2 PF13041 87 - 124 3.1e-09 PPR repeat family
PPR PF01535 90 - 120 1.3e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 256
AclWI GGATC 2 cut(s) 38, 51
AcuI CTGAAG 1 cut(s) 125
AfaI GTAC 1 cut(s) 335
AgsI TTSAA 1 cut(s) 407
AjuI GAANNNNNNNTTGG 2 cut(s) 102, 134
AlwI GGATC 2 cut(s) 38, 51
ApeKI GCWGC 1 cut(s) 218
AsuHPI GGTGA 1 cut(s) 55
BamHI GGATCC 1 cut(s) 43
BanI GGYRCC 1 cut(s) 256
BbvI GCAGC 1 cut(s) 230
BccI CCATC 3 cut(s) 77, 179, 202
BceAI ACGGC 1 cut(s) 330
BclI TGATCA 1 cut(s) 25
BfmI CTRYAG 3 cut(s) 67, 240, 298
BisI GCNGC 1 cut(s) 219
BlsI GCNGC 1 cut(s) 220
BmiI GGNNCC 3 cut(s) 45, 258, 416
BmsI GCATC 3 cut(s) 126, 183, 358
BoxI GACNNNNGTC 1 cut(s) 249
BplI GAGNNNNNCTC 2 cut(s) 136, 168
BsaJI CCNNGG 1 cut(s) 324
BsaWI WCCGGW 1 cut(s) 411
BseDI CCNNGG 1 cut(s) 324
BseGI GGATG 3 cut(s) 174, 268, 390
BseXI GCAGC 1 cut(s) 230
Bsh1285I CGRYCG 1 cut(s) 340
BshNI GGYRCC 1 cut(s) 256
BsiEI CGRYCG 1 cut(s) 340
BsiSI CCGG 2 cut(s) 260, 412
Bsp143I GATC 2 cut(s) 25, 43
Bsp19I CCATGG 1 cut(s) 324
BspLI GGNNCC 3 cut(s) 45, 258, 416
BspMAI CTGCAG 1 cut(s) 302
BspPI GGATC 2 cut(s) 38, 51
BspT107I GGYRCC 1 cut(s) 256
BssECI CCNNGG 1 cut(s) 324
BssMI GATC 2 cut(s) 25, 43
BssT1I CCWWGG 1 cut(s) 324
Bst4CI ACNGT 1 cut(s) 338
Bst6I CTCTTC 2 cut(s) 33, 126
BstAPI GCANNNNNTGC 1 cut(s) 224
BstDEI CTNAG 1 cut(s) 350
BstDSI CCRYGG 1 cut(s) 324
BstEII GGTNACC 1 cut(s) 61
BstF5I GGATG 3 cut(s) 174, 268, 390
BstKTI GATC 2 cut(s) 28, 46
BstMBI GATC 2 cut(s) 25, 43
BstMCI CGRYCG 1 cut(s) 340
BstMWI GCNNNNNNNGC 1 cut(s) 224
BstPAI GACNNNNGTC 1 cut(s) 249
BstPI GGTNACC 1 cut(s) 61
BstSFI CTRYAG 3 cut(s) 67, 240, 298
BstV1I GCAGC 1 cut(s) 230
BstX2I RGATCY 1 cut(s) 43
BstYI RGATCY 1 cut(s) 43
BtgI CCRYGG 1 cut(s) 324
BtsCI GGATG 3 cut(s) 174, 268, 390
BtsI GCAGTG 1 cut(s) 18
BtsIMutI CAGTG 1 cut(s) 18
Csp6I GTAC 1 cut(s) 334
CviAII CATG 3 cut(s) 106, 290, 325
CviJI RGCY 2 cut(s) 221, 323
CviKI_1 RGCY 2 cut(s) 221, 323
CviQI GTAC 1 cut(s) 334
DdeI CTNAG 1 cut(s) 350
DpnI GATC 2 cut(s) 27, 45
DpnII GATC 2 cut(s) 25, 43
DraI TTTAAA 1 cut(s) 94
Eam1104I CTCTTC 2 cut(s) 33, 126
EarI CTCTTC 2 cut(s) 33, 126
Eco130I CCWWGG 1 cut(s) 324
Eco57I CTGAAG 1 cut(s) 125
Eco91I GGTNACC 1 cut(s) 61
EcoO65I GGTNACC 1 cut(s) 61
EcoT14I CCWWGG 1 cut(s) 324
ErhI CCWWGG 1 cut(s) 324
FaeI CATG 3 cut(s) 109, 293, 328
FalI AAGNNNNNCTT 4 cut(s) 123, 155, 207, 239
FatI CATG 3 cut(s) 105, 289, 324
FauNDI CATATG 1 cut(s) 274
FbaI TGATCA 1 cut(s) 25
Fnu4HI GCNGC 1 cut(s) 219
FokI GGATG 3 cut(s) 161, 275, 397
Fsp4HI GCNGC 1 cut(s) 219
GluI GCNGC 1 cut(s) 219
HapII CCGG 2 cut(s) 260, 412
Hin1II CATG 3 cut(s) 109, 293, 328
HinfI GANTC 2 cut(s) 280, 286
HpaII CCGG 2 cut(s) 260, 412
HphI GGTGA 1 cut(s) 55
Hpy188I TCNGA 4 cut(s) 100, 144, 285, 427
Hpy188III TCNNGA 2 cut(s) 47, 404
HpyAV CCTTC 1 cut(s) 410
HpyCH4III ACNGT 1 cut(s) 338
HpyCH4V TGCA 5 cut(s) 11, 195, 227, 300, 392
HpyF10VI GCNNNNNNNGC 1 cut(s) 224
HpyF3I CTNAG 1 cut(s) 350
Hsp92II CATG 3 cut(s) 109, 293, 328
Ksp22I TGATCA 1 cut(s) 25
Kzo9I GATC 2 cut(s) 25, 43
LpnPI CCDG 9 cut(s) 20, 60, 89, 232, 239, 273, 286, 378, 425
Lsp1109I GCAGC 1 cut(s) 230
LweI GCATC 3 cut(s) 126, 183, 358
MaeIII GTNAC 4 cut(s) 61, 167, 293, 345
MalI GATC 2 cut(s) 27, 45
MboI GATC 2 cut(s) 25, 43
MboII GAAGA 4 cut(s) 17, 50, 143, 321
MflI RGATCY 1 cut(s) 43
MmeI TCCRAC 1 cut(s) 286
MnlI CCTC 3 cut(s) 34, 188, 194
MseI TTAA 2 cut(s) 93, 439
MspI CCGG 2 cut(s) 260, 412
MwoI GCNNNNNNNGC 1 cut(s) 224
NcoI CCATGG 1 cut(s) 324
NdeI CATATG 1 cut(s) 274
NdeII GATC 2 cut(s) 25, 43
NlaIII CATG 3 cut(s) 109, 293, 328
NlaIV GGNNCC 3 cut(s) 45, 258, 416
NmuCI GTSAC 1 cut(s) 61
PfeI GAWTC 2 cut(s) 280, 286
PkrI GCNGC 1 cut(s) 220
PshAI GACNNNNGTC 1 cut(s) 249
PspEI GGTNACC 1 cut(s) 61
PspN4I GGNNCC 3 cut(s) 45, 258, 416
PstI CTGCAG 1 cut(s) 302
PsuI RGATCY 1 cut(s) 43
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
SaqAI TTAA 2 cut(s) 93, 439
SatI GCNGC 1 cut(s) 219
Sau3AI GATC 2 cut(s) 25, 43
SetI ASST 3 cut(s) 55, 68, 402
SfaNI GCATC 3 cut(s) 126, 183, 358
SfcI CTRYAG 3 cut(s) 67, 240, 298
StyI CCWWGG 1 cut(s) 324
TaaI ACNGT 1 cut(s) 338
TfiI GAWTC 2 cut(s) 280, 286
Tru1I TTAA 2 cut(s) 93, 439
Tru9I TTAA 2 cut(s) 93, 439
TscAI CASTG 1 cut(s) 18
TseFI GTSAC 1 cut(s) 61
TseI GCWGC 1 cut(s) 218
Tsp45I GTSAC 1 cut(s) 61
TspDTI ATGAA 3 cut(s) 144, 221, 278
TspRI CASTG 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.