MD03G1099200.v1.1

TPL-binding domain in jasmonate signalling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007983
Physical Location & Seq
Reverse (-)
8485060 .. 8488005
2946 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1099200.v1.1.491

Sequence Viewer

Length: 1404 bp
ATGTCTTTCCAGAACAAGGGTTTATGGATGCGAAAGACTGGTGGGCATGTAAATGAGGGAGATGGAAATTTTGGCAACCATTCAAGAATGGAGCCAAAGCGGTCTCAACAATGGTTTGTAGATGCTGGTGAAGCAGAGCTGTTCCCAAACAAGAAGCAAGCAGTACATATTCAAAACAGCAAATTGAGCTCTGGAATGTCAAATGAAAATGTCTCTTCCTGGGAGAATGCTTCCGGTTTCCAGTCAGTTCCCCACCAATTCATTGACCGGTTATTTGGATCTGATACAACAAGCTCTGTGAACTTTGCCGAAAGGAACGTGTCTCCTGTCGGATCAGATAATTGGAATCTAAGGAAGGGTATCGACAACCAGTTTGGAGAAGATGGATCTGTTAGTTTGTCAGTATCTCATGCGATGGAAGATCCTGAAACTCTTAGCTATGCTGGAATTAAAAAAGTCAAAGTCAATCAGGTCAGGGACGGTGACAATGCTATGCATGTTTCGAGGGAATATGGTTCTAATAGGGACAATAATAGTAACCTGTCCACAAATCAGGCATTTGATAGGATAAATGAAACTGGTTTTTTATCGGTAGGGCAGGCCTATGATAAGGAGCACAGTAGTGTCACACTAATGGGTCATGCCTACAACCAGAGGGATGCCCATGGCAGACCAATAGGTCCCAGCTATGGCAAGGGGGATGAAAGTGTGGTATCAATGGCTGACAGTTACAATAAAGGGAATGCAAATTTGATATCTTTTGGTGGGTTTTCTGGTGATCAAGATATTGTAGCCATGGGCAGGCCTGTTGGTAACTATGATCAATTATACCATCCGTCTACAGTTCAAACATCAGAAACTGTGTGCGAGAAGGAATTGGACGTATCAAATGCCAGTGCAGTTGATAATACTGCTTCTGTACCCAAAACAAGACCTGAGTCAGTTTCGAAGAATAAACCTGAGGTCAAAACGTCAAAGAAACAAGCTCCAAACAGCTTCCCGTCAAATGTTAGGAGCTTGATTTCTACGGGCATGCTTGATGGGGTTCCTGTAAAGTATGTTTCCTTGGCACGTGAGCTTCGCGGGATTATAAAAGGTGTTGGCTATCTATGTGGGTGTCAATCATGTAATTATACTAAGGGGCTTAATGCTTACGAGTTTGAGCGCCATGCTGGTTGCAAAACAAAGCATCCAAACAATCACATATTCTTTGAGAATGGTAAGACCATCTATCAGATAGTGCAAGAATTAAGGAGCACGCCTGAGAGCATGTTGTTTGATACACTTCTAACTGTGTTTGGTGCTCCGATCAATCAGAAAGCTTTTAACAGTTGGAAAGAATCATTTCAAGCGGCAACTCGTGAACTTCAGCGTATTTATGGAAAGGAGGAACTGAACTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas

Protein Analysis

468

Amino Acids

51.58

Weight (kDa)

6.68

Isoelectric Point (pI)

34.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 362 - 417 1.7e-14 Tify domain binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1091
AasI GACNNNNNNGTC 1 cut(s) 678
AccI GTMKAC 1 cut(s) 839
AccII CGCG 1 cut(s) 1083
AciI CCGC 3 cut(s) 100, 1083, 1352
AclWI GGATC 4 cut(s) 286, 340, 394, 416
AcsI RAATTY 2 cut(s) 67, 748
AcuI CTGAAG 1 cut(s) 1352
AcvI CACGTG 1 cut(s) 1073
AfaI GTAC 2 cut(s) 165, 921
AfiI CCNNNNNNNGG 3 cut(s) 16, 689, 801
AflIII ACRYGT 1 cut(s) 318
AgeI ACCGGT 1 cut(s) 267
AgsI TTSAA 4 cut(s) 84, 173, 848, 1349
AjnI CCWGG 1 cut(s) 218
AleI CACNNNNGTG 1 cut(s) 621
Alw21I GWGCWC 4 cut(s) 191, 618, 1259, 1306
Alw26I GTCTC 3 cut(s) 108, 217, 327
AlwI GGATC 4 cut(s) 286, 340, 394, 416
AlwNI CAGNNNCTG 1 cut(s) 860
AoxI GGCC 2 cut(s) 600, 803
ApoI RAATTY 2 cut(s) 67, 748
ArsI GACNNNNNNTTYG 4 cut(s) 257, 289, 356, 388
AsiGI ACCGGT 1 cut(s) 267
Asp700I GAANNNNTTC 1 cut(s) 1344
AspLEI GCGC 1 cut(s) 1167
AspS9I GGNCC 1 cut(s) 680
AsuHPI GGTGA 3 cut(s) 140, 494, 788
AsuII TTCGAA 1 cut(s) 947
AvaII GGWCC 1 cut(s) 680
AxyI CCTNAGG 1 cut(s) 960
BanII GRGCYC 1 cut(s) 191
BauI CACGAG 1 cut(s) 1359
BbrPI CACGTG 1 cut(s) 1073
Bbv12I GWGCWC 4 cut(s) 191, 618, 1259, 1306
BccI CCATC 6 cut(s) 56, 377, 409, 840, 1034, 1235
BciT130I CCWGG 1 cut(s) 220
BclI TGATCA 2 cut(s) 778, 820
BcoDI GTCTC 3 cut(s) 108, 217, 327
BfaI CTAG 1 cut(s) 1402
BfmI CTRYAG 1 cut(s) 840
BfoI RGCGCY 1 cut(s) 1168
BisI GCNGC 1 cut(s) 1353
BlsI GCNGC 1 cut(s) 1354
Bme1390I CCNGG 1 cut(s) 220
Bme18I GGWCC 1 cut(s) 680
BmgT120I GGNCC 1 cut(s) 680
BmiI GGNNCC 3 cut(s) 93, 682, 1047
BmrFI CCNGG 1 cut(s) 220
BmsI GCATC 4 cut(s) 18, 112, 649, 1198
BoxI GACNNNNGTC 1 cut(s) 937
Bpu14I TTCGAA 1 cut(s) 947
BsaAI YACGTR 1 cut(s) 1073
BsaI GGTCTC 1 cut(s) 108
BsaJI CCNNGG 4 cut(s) 219, 664, 795, 1065
BsaWI WCCGGW 2 cut(s) 233, 267
Bsc4I CCNNNNNNNGG 3 cut(s) 16, 689, 801
Bse118I RCCGGY 1 cut(s) 267
Bse1I ACTGG 5 cut(s) 43, 241, 370, 583, 894
Bse21I CCTNAGG 1 cut(s) 960
BseBI CCWGG 1 cut(s) 220
BseDI CCNNGG 4 cut(s) 219, 664, 795, 1065
BseGI GGATG 5 cut(s) 33, 664, 706, 832, 1189
BseLI CCNNNNNNNGG 3 cut(s) 16, 689, 801
BseMII CTCAG 3 cut(s) 927, 951, 1254
BseNI ACTGG 5 cut(s) 43, 241, 370, 583, 894
BseYI CCCAGC 1 cut(s) 683
BsgI GTGCAG 1 cut(s) 918
Bsh1236I CGCG 1 cut(s) 1083
BshFI GGCC 2 cut(s) 602, 805
BshTI ACCGGT 1 cut(s) 267
BsiHKAI GWGCWC 4 cut(s) 191, 618, 1259, 1306
BsiSI CCGG 2 cut(s) 234, 268
BslFI GGGAC 3 cut(s) 491, 539, 666
BslI CCNNNNNNNGG 3 cut(s) 16, 689, 801
BsmAI GTCTC 3 cut(s) 108, 217, 327
BsmFI GGGAC 3 cut(s) 491, 539, 666
BsmI GAATGC 2 cut(s) 232, 748
BsnI GGCC 2 cut(s) 602, 805
Bso31I GGTCTC 1 cut(s) 108
Bsp119I TTCGAA 1 cut(s) 947
Bsp1286I GDGCHC 4 cut(s) 191, 618, 1259, 1306
Bsp143I GATC 7 cut(s) 278, 332, 386, 421, 778, 820, 1308
Bsp19I CCATGG 2 cut(s) 664, 795
BspACI CCGC 3 cut(s) 100, 1083, 1352
BspANI GGCC 2 cut(s) 602, 805
BspCNI CTCAG 3 cut(s) 928, 952, 1255
BspFNI CGCG 1 cut(s) 1083
BspLI GGNNCC 3 cut(s) 93, 682, 1047
BspPI GGATC 4 cut(s) 286, 340, 394, 416
BspT104I TTCGAA 1 cut(s) 947
BspTNI GGTCTC 1 cut(s) 108
BsrFI RCCGGY 1 cut(s) 267
BsrI ACTGG 5 cut(s) 43, 241, 370, 583, 894
BssAI RCCGGY 1 cut(s) 267
BssECI CCNNGG 4 cut(s) 219, 664, 795, 1065
BssMI GATC 7 cut(s) 278, 332, 386, 421, 778, 820, 1308
BssSI CACGAG 1 cut(s) 1359
BssT1I CCWWGG 3 cut(s) 664, 795, 1065
Bst2BI CACGAG 1 cut(s) 1359
Bst2UI CCWGG 1 cut(s) 220
Bst4CI ACNGT 7 cut(s) 482, 620, 728, 844, 862, 1294, 1331
Bst6I CTCTTC 1 cut(s) 220
BstBAI YACGTR 1 cut(s) 1073
BstBI TTCGAA 1 cut(s) 947
BstC8I GCNNGC 5 cut(s) 159, 600, 803, 1034, 1259
BstDEI CTNAG 6 cut(s) 350, 434, 936, 960, 1137, 1263
BstDSI CCRYGG 2 cut(s) 664, 795
BstF5I GGATG 5 cut(s) 33, 664, 706, 832, 1189
BstFNI CGCG 1 cut(s) 1083
BstH2I RGCGCY 1 cut(s) 1168
BstHHI GCGC 1 cut(s) 1167
BstKTI GATC 7 cut(s) 281, 335, 389, 424, 781, 823, 1311
BstMAI GTCTC 3 cut(s) 108, 217, 327
BstMBI GATC 7 cut(s) 278, 332, 386, 421, 778, 820, 1308
BstMWI GCNNNNNNNGC 2 cut(s) 131, 186
BstNI CCWGG 1 cut(s) 220
BstNSI RCATGY 4 cut(s) 50, 500, 1036, 1273
BstPAI GACNNNNGTC 1 cut(s) 937
BstSCI CCNGG 1 cut(s) 218
BstSFI CTRYAG 1 cut(s) 840
BstUI CGCG 1 cut(s) 1083
BstX2I RGATCY 3 cut(s) 278, 386, 421
BstYI RGATCY 3 cut(s) 278, 386, 421
Bsu36I CCTNAGG 1 cut(s) 960
BsuRI GGCC 2 cut(s) 602, 805
BtgI CCRYGG 2 cut(s) 664, 795
BtgZI GCGATG 1 cut(s) 428
BtsCI GGATG 5 cut(s) 33, 664, 706, 832, 1189
BtsIMutI CAGTG 1 cut(s) 901
Cac8I GCNNGC 5 cut(s) 159, 600, 803, 1034, 1259
CaiI CAGNNNCTG 1 cut(s) 860
CfoI GCGC 1 cut(s) 1167
Cfr10I RCCGGY 1 cut(s) 267
Cfr13I GGNCC 1 cut(s) 680
Csp6I GTAC 2 cut(s) 164, 920
CspAI ACCGGT 1 cut(s) 267
CviQI GTAC 2 cut(s) 164, 920
DdeI CTNAG 6 cut(s) 350, 434, 936, 960, 1137, 1263
DpnI GATC 7 cut(s) 280, 334, 388, 423, 780, 822, 1310
DpnII GATC 7 cut(s) 278, 332, 386, 421, 778, 820, 1308
DrdI GACNNNNNNGTC 1 cut(s) 678
DseDI GACNNNNNNGTC 1 cut(s) 678
Eam1104I CTCTTC 1 cut(s) 220
EarI CTCTTC 1 cut(s) 220
Ecl136II GAGCTC 1 cut(s) 189
Eco130I CCWWGG 3 cut(s) 664, 795, 1065
Eco147I AGGCCT 2 cut(s) 602, 805
Eco24I GRGCYC 1 cut(s) 191
Eco31I GGTCTC 1 cut(s) 108
Eco32I GATATC 1 cut(s) 756
Eco47I GGWCC 1 cut(s) 680
Eco53kI GAGCTC 1 cut(s) 189
Eco57I CTGAAG 1 cut(s) 1352
Eco72I CACGTG 1 cut(s) 1073
Eco81I CCTNAGG 1 cut(s) 960
EcoICRI GAGCTC 1 cut(s) 189
EcoO109I RGGNCCY 1 cut(s) 680
EcoRII CCWGG 1 cut(s) 218
EcoRV GATATC 1 cut(s) 756
EcoT14I CCWWGG 3 cut(s) 664, 795, 1065
EcoT22I ATGCAT 1 cut(s) 498
EcoT38I GRGCYC 1 cut(s) 191
ErhI CCWWGG 3 cut(s) 664, 795, 1065
FaqI GGGAC 3 cut(s) 491, 539, 666
FauI CCCGC 1 cut(s) 1076
FbaI TGATCA 2 cut(s) 778, 820
FblI GTMKAC 1 cut(s) 839
Fnu4HI GCNGC 1 cut(s) 1353
FokI GGATG 5 cut(s) 40, 671, 713, 819, 1176
FriOI GRGCYC 1 cut(s) 191
Fsp4HI GCNGC 1 cut(s) 1353
FspBI CTAG 1 cut(s) 1402
GlaI GCGC 1 cut(s) 1166
GluI GCNGC 1 cut(s) 1353
GsaI CCCAGC 1 cut(s) 687
HaeII RGCGCY 1 cut(s) 1168
HaeIII GGCC 2 cut(s) 602, 805
HapII CCGG 2 cut(s) 234, 268
HhaI GCGC 1 cut(s) 1167
Hin6I GCGC 1 cut(s) 1165
HinP1I GCGC 1 cut(s) 1165
HindIII AAGCTT 1 cut(s) 1320
HinfI GANTC 3 cut(s) 346, 938, 1340
HpaII CCGG 2 cut(s) 234, 268
HphI GGTGA 3 cut(s) 140, 494, 788
Hpy166II GTNNAC 4 cut(s) 301, 546, 840, 1364
Hpy188I TCNGA 7 cut(s) 283, 332, 337, 856, 1236, 1308, 1317
Hpy188III TCNNGA 6 cut(s) 10, 84, 192, 425, 782, 1361
Hpy8I GTNNAC 4 cut(s) 301, 546, 840, 1364
HpyAV CCTTC 2 cut(s) 349, 865
HpyCH4III ACNGT 7 cut(s) 482, 620, 728, 844, 862, 1294, 1331
HpyCH4IV ACGT 4 cut(s) 318, 882, 971, 1072
HpyCH4V TGCA 5 cut(s) 496, 746, 899, 1179, 1243
HpyF10VI GCNNNNNNNGC 2 cut(s) 131, 186
HpyF3I CTNAG 6 cut(s) 350, 434, 936, 960, 1137, 1263
HpySE526I ACGT 4 cut(s) 318, 882, 971, 1072
HspAI GCGC 1 cut(s) 1165
Ksp22I TGATCA 2 cut(s) 778, 820
Kzo9I GATC 7 cut(s) 278, 332, 386, 421, 778, 820, 1308
LmnI GCTCC 6 cut(s) 91, 613, 991, 1014, 1254, 1309
LweI GCATC 4 cut(s) 18, 112, 649, 1198
MaeI CTAG 1 cut(s) 1402
MaeII ACGT 4 cut(s) 318, 882, 971, 1072
MaeIII GTNAC 5 cut(s) 482, 536, 625, 728, 812
MalI GATC 7 cut(s) 280, 334, 388, 423, 780, 822, 1310
MboI GATC 7 cut(s) 278, 332, 386, 421, 778, 820, 1308
MboII GAAGA 4 cut(s) 207, 392, 431, 961
MflI RGATCY 3 cut(s) 278, 386, 421
MhlI GDGCHC 4 cut(s) 191, 618, 1259, 1306
MlyI GAGTC 1 cut(s) 947
MmeI TCCRAC 2 cut(s) 310, 1313
MnlI CCTC 5 cut(s) 49, 498, 648, 955, 1381
Mph1103I ATGCAT 1 cut(s) 498
MroXI GAANNNNTTC 1 cut(s) 1344
MseI TTAA 4 cut(s) 450, 1146, 1250, 1326
MslI CAYNNNNRTG 3 cut(s) 51, 621, 632
MspI CCGG 2 cut(s) 234, 268
MspR9I CCNGG 1 cut(s) 220
Mva1269I GAATGC 2 cut(s) 232, 748
MvaI CCWGG 1 cut(s) 220
MvnI CGCG 1 cut(s) 1083
MwoI GCNNNNNNNGC 2 cut(s) 131, 186
NcoI CCATGG 2 cut(s) 664, 795
NdeII GATC 7 cut(s) 278, 332, 386, 421, 778, 820, 1308
NlaIV GGNNCC 3 cut(s) 93, 682, 1047
NmuCI GTSAC 2 cut(s) 482, 625
NsiI ATGCAT 1 cut(s) 498
NspI RCATGY 4 cut(s) 50, 500, 1036, 1273
NspV TTCGAA 1 cut(s) 947
OliI CACNNNNGTG 1 cut(s) 621
PaeI GCATGC 1 cut(s) 1036
PceI AGGCCT 2 cut(s) 602, 805
PctI GAATGC 2 cut(s) 232, 748
PdmI GAANNNNTTC 1 cut(s) 1344
PfeI GAWTC 2 cut(s) 346, 1340
PinAI ACCGGT 1 cut(s) 267
PkrI GCNGC 1 cut(s) 1354
PleI GAGTC 1 cut(s) 946
PmaCI CACGTG 1 cut(s) 1073
PmlI CACGTG 1 cut(s) 1073
PpsI GAGTC 1 cut(s) 946
Ppu21I YACGTR 1 cut(s) 1073
PpuMI RGGWCCY 1 cut(s) 680
PshAI GACNNNNGTC 1 cut(s) 937
PsiI TTATAA 1 cut(s) 1091
Psp124BI GAGCTC 1 cut(s) 191
Psp5II RGGWCCY 1 cut(s) 680
Psp6I CCWGG 1 cut(s) 218
PspCI CACGTG 1 cut(s) 1073
PspFI CCCAGC 1 cut(s) 683
PspGI CCWGG 1 cut(s) 218
PspN4I GGNNCC 3 cut(s) 93, 682, 1047
PspPI GGNCC 1 cut(s) 680
PspPPI RGGWCCY 1 cut(s) 680
PstNI CAGNNNCTG 1 cut(s) 860
PsuI RGATCY 3 cut(s) 278, 386, 421
RsaI GTAC 2 cut(s) 165, 921
RsaNI GTAC 2 cut(s) 164, 920
RseI CAYNNNNRTG 3 cut(s) 51, 621, 632
SacI GAGCTC 1 cut(s) 191
SaqAI TTAA 4 cut(s) 450, 1146, 1250, 1326
SatI GCNGC 1 cut(s) 1353
Sau3AI GATC 7 cut(s) 278, 332, 386, 421, 778, 820, 1308
Sau96I GGNCC 1 cut(s) 680
SchI GAGTC 1 cut(s) 947
ScrFI CCNGG 1 cut(s) 220
SduI GDGCHC 4 cut(s) 191, 618, 1259, 1306
SfaNI GCATC 4 cut(s) 18, 112, 649, 1198
SfcI CTRYAG 1 cut(s) 840
SfuI TTCGAA 1 cut(s) 947
SinI GGWCC 1 cut(s) 680
SmiMI CAYNNNNRTG 3 cut(s) 51, 621, 632
SphI GCATGC 1 cut(s) 1036
SseBI AGGCCT 2 cut(s) 602, 805
SsiI CCGC 3 cut(s) 100, 1083, 1352
SspMI CTAG 1 cut(s) 1402
SstI GAGCTC 1 cut(s) 191
StuI AGGCCT 2 cut(s) 602, 805
StyD4I CCNGG 1 cut(s) 218
StyI CCWWGG 3 cut(s) 664, 795, 1065
TaaI ACNGT 7 cut(s) 482, 620, 728, 844, 862, 1294, 1331
TaiI ACGT 4 cut(s) 321, 885, 974, 1075
TaqI TCGA 3 cut(s) 363, 503, 947
TatI WGTACW 1 cut(s) 163
TauI GCSGC 1 cut(s) 1355
TfiI GAWTC 2 cut(s) 346, 1340
Tru1I TTAA 4 cut(s) 450, 1146, 1250, 1326
Tru9I TTAA 4 cut(s) 450, 1146, 1250, 1326
TscAI CASTG 1 cut(s) 901
TseFI GTSAC 2 cut(s) 482, 625
Tsp45I GTSAC 2 cut(s) 482, 625
TspDTI ATGAA 4 cut(s) 219, 250, 588, 717
TspGWI ACGGA 1 cut(s) 825
TspRI CASTG 1 cut(s) 901
VpaK11BI GGWCC 1 cut(s) 680
XapI RAATTY 2 cut(s) 67, 748
XceI RCATGY 4 cut(s) 50, 500, 1036, 1273
XmiI GTMKAC 1 cut(s) 839
XmnI GAANNNNTTC 1 cut(s) 1344
XspI CTAG 1 cut(s) 1402
Zsp2I ATGCAT 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.