MD11G1113100.v1.1

TPL-binding domain in jasmonate signalling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007983
Physical Location & Seq
Reverse (-)
10225079 .. 10228005
2927 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1113100.v1.1.491

Sequence Viewer

Length: 1401 bp
ATGTCTTTCCAGAACAAGGGTTTTTGGATGCCAAAGAGCGGCGGATATGTAAATGAGGGAGAGGGAACTTTTGGCAACCCATCAAGAATTGAGCCAAAGCGATCTCAGCAATGGTTTGTAGATGCTGGTGAAGCAGAGCTGTTTCCAAACAAGAAGCAAGCAGTACATATTCCAAACAGAAAATTGAGCTCGGAAATGTCAAATGAAAATGTCCCTTCTTGGGAGAATGCTTCAGGCTTCCAGTCAGTTCCGCATCAATTCATTGACCGGTTATTTGGATCTGATACAGCAAGCTCTGTCAGCCTTGCTGAAAGGAACGTGTCTCCTGTCGGATCAGGTAATTGGAATCTAAGGAAGGGTATCGGTGACCAGTTTGGAGAAGTTGGATCTGTTAGTTTGTCAGTATCTCATGCTATGGAAGATCCTGAAACTCTTAGCTATGCTGGAATTAGAAAAGTCAAAGTCAATCAGGTCAGGGACGGTGACAATGGTATGCATGCGTCGAGGGAAAATGGTTCCAATAGGGAAAATAATAGTAACCTGTCCACAAATCAGGCATTTGATAGGGTAAATGAAACTGGTTTTTTATCAGTGGGGCAGGCCTATGATAAGGAACACGGTAGTGTCTCATTAATGGGTCATGCGTACAACCAGAGGGATGACCATGGCAGACCAATAGGTCCCAACTATGGCAAGGGGGATGAAAGTGTGGTAGCATTGGCTGACAGTTATAATAAAGGGAATGCAAGTATGGTATCTTTTGGGGTGTTTCCTGGTGATCAAGATGTAGCCATGGGAAGACTCGTTGGTAACTATGATCAATTATACCATCCTTCTTCAGTTCAAATATCAGAAACTGCGTGCGAGAAGGAATTGGACACATCAAATGCCAGTGCAGTTGATAATACTGCCTTTGTAGCCAAAACAAGACCTGAGTCAGTTTTGAAGAATAAACCCGAGGTCAAAACGTCAAGGAAACAAGCTCCAAACAGCTTCCCGTCGAATGTTAGGAGCTTGATTTCTACGGGCATGCTTGATGGGGTTCCTGTAAAATATGTTTCCTTGGCACGTGAGCTTCGAGGGATTATCAAAGGTGTCGGCTATCTATGTGGGTGTCAATCATGTAATTATGCTAAGGTGCTTAATGCTTACGAGTTTGAGCGCCATGCTGGTTGCAAAACAAAGCATCCAAACAATCACATATTCTTTGAGAACGGCAAGACCATCTATCAGATAGTGCAGGAATTAAAGAGCACGCCTGAGAGCTTGTTGTTTGATGCACTTCAAACTGTGTTTGGTGCTCCGATCAATCAGAAAGCTTTTAAAAGTTGGAAAGAATCCTTTCAAGCGGCAACTCGTGAACTTAAGCGTATTTATGGAAAGGAGGAACTGAACTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas

Protein Analysis

467

Amino Acids

51.26

Weight (kDa)

7.17

Isoelectric Point (pI)

36.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 361 - 416 7.3e-16 Tify domain binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 732
AasI GACNNNNNNGTC 1 cut(s) 678
AccBSI CCGCTC 1 cut(s) 39
AciI CCGC 4 cut(s) 39, 42, 251, 1349
AclWI GGATC 4 cut(s) 286, 340, 394, 416
AcuI CTGAAG 2 cut(s) 216, 822
AcvI CACGTG 1 cut(s) 1070
AfaI GTAC 2 cut(s) 165, 647
AfiI CCNNNNNNNGG 4 cut(s) 16, 38, 220, 689
AflII CTTAAG 1 cut(s) 1364
AflIII ACRYGT 1 cut(s) 318
AgeI ACCGGT 1 cut(s) 267
AgsI TTSAA 4 cut(s) 845, 946, 1286, 1346
AjnI CCWGG 1 cut(s) 772
AleI CACNNNNGTG 1 cut(s) 621
Alw21I GWGCWC 3 cut(s) 191, 1256, 1303
Alw26I GTCTC 2 cut(s) 327, 631
AlwI GGATC 4 cut(s) 286, 340, 394, 416
AlwNI CAGNNNCTG 1 cut(s) 857
Ama87I CYCGRG 1 cut(s) 956
AoxI GGCC 1 cut(s) 600
ArsI GACNNNNNNTTYG 2 cut(s) 257, 289
AseI ATTAAT 1 cut(s) 632
AsiGI ACCGGT 1 cut(s) 267
Asp700I GAANNNNTTC 1 cut(s) 1341
AspLEI GCGC 1 cut(s) 1164
AspS9I GGNCC 1 cut(s) 680
AsuHPI GGTGA 4 cut(s) 140, 377, 494, 788
AvaI CYCGRG 1 cut(s) 956
AvaII GGWCC 1 cut(s) 680
BanII GRGCYC 1 cut(s) 191
BauI CACGAG 1 cut(s) 1356
BbrPI CACGTG 1 cut(s) 1070
BbsI GAAGAC 1 cut(s) 805
Bbv12I GWGCWC 3 cut(s) 191, 1256, 1303
BccI CCATC 4 cut(s) 88, 837, 1031, 1232
BceAI ACGGC 1 cut(s) 1231
BciT130I CCWGG 1 cut(s) 774
BclI TGATCA 2 cut(s) 778, 817
BcoDI GTCTC 2 cut(s) 327, 631
BfoI RGCGCY 1 cut(s) 1165
BfrI CTTAAG 1 cut(s) 1364
BisI GCNGC 2 cut(s) 40, 1350
BlsI GCNGC 2 cut(s) 41, 1351
Bme1390I CCNGG 1 cut(s) 774
Bme18I GGWCC 1 cut(s) 680
BmeT110I CYCGRG 1 cut(s) 956
BmgT120I GGNCC 1 cut(s) 680
BmiI GGNNCC 3 cut(s) 517, 682, 1044
BmrFI CCNGG 1 cut(s) 774
BmsI GCATC 5 cut(s) 18, 112, 262, 1195, 1267
BoxI GACNNNNGTC 1 cut(s) 934
BpiI GAAGAC 1 cut(s) 805
Bpu10I CCTNAGC 1 cut(s) 1134
BsaAI YACGTR 1 cut(s) 1070
BsaJI CCNNGG 4 cut(s) 664, 792, 957, 1062
BsaWI WCCGGW 1 cut(s) 267
Bsc4I CCNNNNNNNGG 4 cut(s) 16, 38, 220, 689
Bse118I RCCGGY 1 cut(s) 267
Bse1I ACTGG 4 cut(s) 241, 370, 583, 891
Bse3DI GCAATG 1 cut(s) 116
BseBI CCWGG 1 cut(s) 774
BseDI CCNNGG 4 cut(s) 664, 792, 957, 1062
BseGI GGATG 5 cut(s) 33, 664, 706, 829, 1186
BseLI CCNNNNNNNGG 4 cut(s) 16, 38, 220, 689
BseMI GCAATG 1 cut(s) 116
BseMII CTCAG 3 cut(s) 119, 924, 1251
BseNI ACTGG 4 cut(s) 241, 370, 583, 891
BsgI GTGCAG 2 cut(s) 915, 1259
BshFI GGCC 1 cut(s) 602
BshTI ACCGGT 1 cut(s) 267
BsiHKAI GWGCWC 3 cut(s) 191, 1256, 1303
BsiHKCI CYCGRG 1 cut(s) 956
BsiSI CCGG 1 cut(s) 268
BslFI GGGAC 3 cut(s) 197, 491, 666
BslI CCNNNNNNNGG 4 cut(s) 16, 38, 220, 689
BsmAI GTCTC 2 cut(s) 327, 631
BsmFI GGGAC 3 cut(s) 197, 491, 666
BsmI GAATGC 2 cut(s) 232, 748
BsnI GGCC 1 cut(s) 602
BsoBI CYCGRG 1 cut(s) 956
Bsp1286I GDGCHC 3 cut(s) 191, 1256, 1303
Bsp143I GATC 8 cut(s) 101, 278, 332, 386, 421, 778, 817, 1305
Bsp19I CCATGG 2 cut(s) 664, 792
BspACI CCGC 4 cut(s) 39, 42, 251, 1349
BspANI GGCC 1 cut(s) 602
BspCNI CTCAG 3 cut(s) 118, 925, 1252
BspLI GGNNCC 3 cut(s) 517, 682, 1044
BspPI GGATC 4 cut(s) 286, 340, 394, 416
BspTI CTTAAG 1 cut(s) 1364
BsrBI CCGCTC 1 cut(s) 39
BsrDI GCAATG 1 cut(s) 116
BsrFI RCCGGY 1 cut(s) 267
BsrI ACTGG 4 cut(s) 241, 370, 583, 891
BssAI RCCGGY 1 cut(s) 267
BssECI CCNNGG 4 cut(s) 664, 792, 957, 1062
BssMI GATC 8 cut(s) 101, 278, 332, 386, 421, 778, 817, 1305
BssSI CACGAG 1 cut(s) 1356
BssT1I CCWWGG 3 cut(s) 664, 792, 1062
Bst2BI CACGAG 1 cut(s) 1356
Bst2UI CCWGG 1 cut(s) 774
Bst4CI ACNGT 4 cut(s) 482, 620, 728, 1291
BstAFI CTTAAG 1 cut(s) 1364
BstBAI YACGTR 1 cut(s) 1070
BstC8I GCNNGC 7 cut(s) 159, 292, 498, 600, 862, 1031, 1256
BstDEI CTNAG 6 cut(s) 105, 350, 434, 933, 1134, 1260
BstDSI CCRYGG 2 cut(s) 664, 792
BstEII GGTNACC 1 cut(s) 365
BstF5I GGATG 5 cut(s) 33, 664, 706, 829, 1186
BstH2I RGCGCY 1 cut(s) 1165
BstHHI GCGC 1 cut(s) 1164
BstKTI GATC 8 cut(s) 104, 281, 335, 389, 424, 781, 820, 1308
BstMAI GTCTC 2 cut(s) 327, 631
BstMBI GATC 8 cut(s) 101, 278, 332, 386, 421, 778, 817, 1305
BstMWI GCNNNNNNNGC 4 cut(s) 106, 131, 300, 917
BstNI CCWGG 1 cut(s) 774
BstNSI RCATGY 2 cut(s) 500, 1033
BstPAI GACNNNNGTC 1 cut(s) 934
BstPI GGTNACC 1 cut(s) 365
BstSCI CCNGG 1 cut(s) 772
BstV2I GAAGAC 1 cut(s) 805
BstX2I RGATCY 3 cut(s) 278, 386, 421
BstYI RGATCY 3 cut(s) 278, 386, 421
BsuRI GGCC 1 cut(s) 602
BtgI CCRYGG 2 cut(s) 664, 792
BtsCI GGATG 5 cut(s) 33, 664, 706, 829, 1186
BtsIMutI CAGTG 2 cut(s) 597, 898
Cac8I GCNNGC 7 cut(s) 159, 292, 498, 600, 862, 1031, 1256
CaiI CAGNNNCTG 1 cut(s) 857
CfoI GCGC 1 cut(s) 1164
Cfr10I RCCGGY 1 cut(s) 267
Cfr13I GGNCC 1 cut(s) 680
CseI GACGC 1 cut(s) 489
Csp6I GTAC 2 cut(s) 164, 646
CspAI ACCGGT 1 cut(s) 267
CviAII CATG 8 cut(s) 410, 497, 641, 665, 793, 1030, 1122, 1166
CviQI GTAC 2 cut(s) 164, 646
DdeI CTNAG 6 cut(s) 105, 350, 434, 933, 1134, 1260
DpnI GATC 8 cut(s) 103, 280, 334, 388, 423, 780, 819, 1307
DpnII GATC 8 cut(s) 101, 278, 332, 386, 421, 778, 817, 1305
DraI TTTAAA 1 cut(s) 1324
DrdI GACNNNNNNGTC 1 cut(s) 678
DseDI GACNNNNNNGTC 1 cut(s) 678
EciI GGCGGA 1 cut(s) 57
Ecl136II GAGCTC 1 cut(s) 189
Eco130I CCWWGG 3 cut(s) 664, 792, 1062
Eco147I AGGCCT 1 cut(s) 602
Eco24I GRGCYC 1 cut(s) 191
Eco47I GGWCC 1 cut(s) 680
Eco53kI GAGCTC 1 cut(s) 189
Eco57I CTGAAG 2 cut(s) 216, 822
Eco72I CACGTG 1 cut(s) 1070
Eco88I CYCGRG 1 cut(s) 956
Eco91I GGTNACC 1 cut(s) 365
EcoICRI GAGCTC 1 cut(s) 189
EcoO109I RGGNCCY 1 cut(s) 680
EcoO65I GGTNACC 1 cut(s) 365
EcoRII CCWGG 1 cut(s) 772
EcoT14I CCWWGG 3 cut(s) 664, 792, 1062
EcoT22I ATGCAT 1 cut(s) 498
EcoT38I GRGCYC 1 cut(s) 191
ErhI CCWWGG 3 cut(s) 664, 792, 1062
FaeI CATG 8 cut(s) 413, 500, 644, 668, 796, 1033, 1125, 1169
FaqI GGGAC 3 cut(s) 197, 491, 666
FatI CATG 8 cut(s) 409, 496, 640, 664, 792, 1029, 1121, 1165
FbaI TGATCA 2 cut(s) 778, 817
Fnu4HI GCNGC 2 cut(s) 40, 1350
FokI GGATG 5 cut(s) 40, 671, 713, 816, 1173
FriOI GRGCYC 1 cut(s) 191
Fsp4HI GCNGC 2 cut(s) 40, 1350
GlaI GCGC 1 cut(s) 1163
GluI GCNGC 2 cut(s) 40, 1350
HaeII RGCGCY 1 cut(s) 1165
HaeIII GGCC 1 cut(s) 602
HapII CCGG 1 cut(s) 268
HgaI GACGC 1 cut(s) 489
HhaI GCGC 1 cut(s) 1164
Hin1II CATG 8 cut(s) 413, 500, 644, 668, 796, 1033, 1125, 1169
Hin6I GCGC 1 cut(s) 1162
HinP1I GCGC 1 cut(s) 1162
HindIII AAGCTT 1 cut(s) 1317
HinfI GANTC 4 cut(s) 346, 801, 935, 1337
HpaII CCGG 1 cut(s) 268
HphI GGTGA 4 cut(s) 140, 377, 494, 788
Hpy166II GTNNAC 2 cut(s) 546, 1361
Hpy188I TCNGA 7 cut(s) 193, 283, 332, 853, 1233, 1305, 1314
Hpy188III TCNNGA 5 cut(s) 10, 84, 425, 782, 1358
Hpy8I GTNNAC 2 cut(s) 546, 1361
Hpy99I CGWCG 2 cut(s) 505, 1003
HpyAV CCTTC 4 cut(s) 225, 349, 843, 862
HpyCH4III ACNGT 4 cut(s) 482, 620, 728, 1291
HpyCH4IV ACGT 3 cut(s) 318, 968, 1069
HpyCH4V TGCA 6 cut(s) 496, 746, 896, 1176, 1240, 1280
HpyF10VI GCNNNNNNNGC 4 cut(s) 106, 131, 300, 917
HpyF3I CTNAG 6 cut(s) 105, 350, 434, 933, 1134, 1260
HpySE526I ACGT 3 cut(s) 318, 968, 1069
Hsp92II CATG 8 cut(s) 413, 500, 644, 668, 796, 1033, 1125, 1169
HspAI GCGC 1 cut(s) 1162
Ksp22I TGATCA 2 cut(s) 778, 817
Kzo9I GATC 8 cut(s) 101, 278, 332, 386, 421, 778, 817, 1305
LmnI GCTCC 3 cut(s) 988, 1011, 1306
LweI GCATC 5 cut(s) 18, 112, 262, 1195, 1267
MaeII ACGT 3 cut(s) 318, 968, 1069
MaeIII GTNAC 4 cut(s) 365, 482, 536, 809
MalI GATC 8 cut(s) 103, 280, 334, 388, 423, 780, 819, 1307
MbiI CCGCTC 1 cut(s) 39
MboI GATC 8 cut(s) 101, 278, 332, 386, 421, 778, 817, 1305
MboII GAAGA 4 cut(s) 431, 810, 828, 958
MflI RGATCY 3 cut(s) 278, 386, 421
MhlI GDGCHC 3 cut(s) 191, 1256, 1303
MluCI AATT 9 cut(s) 87, 182, 257, 340, 447, 821, 872, 1126, 1244
MlyI GAGTC 2 cut(s) 795, 944
MmeI TCCRAC 3 cut(s) 310, 364, 1310
MnlI CCTC 7 cut(s) 49, 55, 498, 648, 952, 1073, 1378
Mph1103I ATGCAT 1 cut(s) 498
MroXI GAANNNNTTC 1 cut(s) 1341
MseI TTAA 5 cut(s) 632, 1143, 1247, 1323, 1365
MslI CAYNNNNRTG 1 cut(s) 621
MspCI CTTAAG 1 cut(s) 1364
MspI CCGG 1 cut(s) 268
MspR9I CCNGG 1 cut(s) 774
Mva1269I GAATGC 2 cut(s) 232, 748
MvaI CCWGG 1 cut(s) 774
MwoI GCNNNNNNNGC 4 cut(s) 106, 131, 300, 917
NcoI CCATGG 2 cut(s) 664, 792
NdeII GATC 8 cut(s) 101, 278, 332, 386, 421, 778, 817, 1305
NlaIII CATG 8 cut(s) 413, 500, 644, 668, 796, 1033, 1125, 1169
NlaIV GGNNCC 3 cut(s) 517, 682, 1044
NmuCI GTSAC 2 cut(s) 365, 482
NsiI ATGCAT 1 cut(s) 498
NspI RCATGY 2 cut(s) 500, 1033
OliI CACNNNNGTG 1 cut(s) 621
PaeI GCATGC 2 cut(s) 500, 1033
PceI AGGCCT 1 cut(s) 602
PcsI WCGNNNNNNNCGW 1 cut(s) 1075
PctI GAATGC 2 cut(s) 232, 748
PdmI GAANNNNTTC 1 cut(s) 1341
PfeI GAWTC 2 cut(s) 346, 1337
PinAI ACCGGT 1 cut(s) 267
PkrI GCNGC 2 cut(s) 41, 1351
PleI GAGTC 2 cut(s) 795, 943
PmaCI CACGTG 1 cut(s) 1070
PmlI CACGTG 1 cut(s) 1070
PpsI GAGTC 2 cut(s) 795, 943
Ppu21I YACGTR 1 cut(s) 1070
PpuMI RGGWCCY 1 cut(s) 680
PshAI GACNNNNGTC 1 cut(s) 934
PshBI ATTAAT 1 cut(s) 632
PsiI TTATAA 1 cut(s) 732
Psp124BI GAGCTC 1 cut(s) 191
Psp5II RGGWCCY 1 cut(s) 680
Psp6I CCWGG 1 cut(s) 772
PspCI CACGTG 1 cut(s) 1070
PspEI GGTNACC 1 cut(s) 365
PspGI CCWGG 1 cut(s) 772
PspN4I GGNNCC 3 cut(s) 517, 682, 1044
PspPI GGNCC 1 cut(s) 680
PspPPI RGGWCCY 1 cut(s) 680
PstNI CAGNNNCTG 1 cut(s) 857
PsuI RGATCY 3 cut(s) 278, 386, 421
RsaI GTAC 2 cut(s) 165, 647
RsaNI GTAC 2 cut(s) 164, 646
RseI CAYNNNNRTG 1 cut(s) 621
SacI GAGCTC 1 cut(s) 191
SaqAI TTAA 5 cut(s) 632, 1143, 1247, 1323, 1365
SatI GCNGC 2 cut(s) 40, 1350
Sau3AI GATC 8 cut(s) 101, 278, 332, 386, 421, 778, 817, 1305
Sau96I GGNCC 1 cut(s) 680
SchI GAGTC 2 cut(s) 795, 944
ScrFI CCNGG 1 cut(s) 774
SduI GDGCHC 3 cut(s) 191, 1256, 1303
SfaNI GCATC 5 cut(s) 18, 112, 262, 1195, 1267
SinI GGWCC 1 cut(s) 680
SmiMI CAYNNNNRTG 1 cut(s) 621
SmlI CTYRAG 1 cut(s) 1364
SmoI CTYRAG 1 cut(s) 1364
SphI GCATGC 2 cut(s) 500, 1033
Sse9I AATT 9 cut(s) 87, 182, 257, 340, 447, 821, 872, 1126, 1244
SseBI AGGCCT 1 cut(s) 602
SsiI CCGC 4 cut(s) 39, 42, 251, 1349
SstI GAGCTC 1 cut(s) 191
StuI AGGCCT 1 cut(s) 602
StyD4I CCNGG 1 cut(s) 772
StyI CCWWGG 3 cut(s) 664, 792, 1062
TaaI ACNGT 4 cut(s) 482, 620, 728, 1291
TaiI ACGT 3 cut(s) 321, 971, 1072
TaqI TCGA 3 cut(s) 503, 1001, 1078
TasI AATT 9 cut(s) 87, 182, 257, 340, 447, 821, 872, 1126, 1244
TatI WGTACW 1 cut(s) 163
TauI GCSGC 2 cut(s) 42, 1352
TfiI GAWTC 2 cut(s) 346, 1337
Tru1I TTAA 5 cut(s) 632, 1143, 1247, 1323, 1365
Tru9I TTAA 5 cut(s) 632, 1143, 1247, 1323, 1365
TscAI CASTG 2 cut(s) 597, 898
TseFI GTSAC 2 cut(s) 365, 482
Tsp45I GTSAC 2 cut(s) 365, 482
TspDTI ATGAA 4 cut(s) 219, 250, 588, 717
TspRI CASTG 2 cut(s) 597, 898
Vha464I CTTAAG 1 cut(s) 1364
VpaK11BI GGWCC 1 cut(s) 680
VspI ATTAAT 1 cut(s) 632
XceI RCATGY 2 cut(s) 500, 1033
XmnI GAANNNNTTC 1 cut(s) 1341
Zsp2I ATGCAT 1 cut(s) 498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.