Rorug05G0354100

TPL-binding domain in jasmonate signalling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0007983
Physical Location & Seq
Reverse (-)
44243890 .. 44249500
5611 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0354100.1

Sequence Viewer

Length: 768 bp
ATGGAAACCGACGAAGAAGAACAACGCGTCACTCCATTCCAGCTCCAGTTCGACAAGCCAGTCGCTTCGCAGATTAAGATCGCGGAGTGGAATCCCGAGAAGGATTTACTCGCCATGAGGCTCTGGACTATCTCCCCAGGGAAGAGCATAACGTCTTTGTGTTGGAGACCTGATGGTAAAGCAATCGCCGTCGGGCTCGAAGATGGAGCAGTTTCATTGCATGATGTTGAATTTTCGAACTTCTTCAATTGGCTCTTGAAGTGTATAAAGTTACTCAATTCAGAACCCAGTGATCATCATCTTCTACTGTACAATAGTGAACTTGTTGTTATATTCTTGAAGTTTCTATATGACCAAGATCCTGTTAAGCAATTGCTGGAGGCGTCGGAAGCTGATGAGAGTATAGAAGTTGGATTGGAAACAGTGCAGAGAGTTAGAGAATTAGTTCAGTTTGGGGGATTTTCAGATTGTGAGCACCTGCAGAGGACGTTAGCCAAGGAATTTCAACAGATGGAGTCTAGTTTTAAGGAGGCTTTCCAGATGCCCTTTACTACAATTTCAAGAAAGATAGTCTGCGAAGATTCATTGCCGCTTTGCCCACTTCCACCTTTGTCAGCATCTTTGTCTACCAGTATTTCTATGTCAGTATCATATTATGAGGATGCCTCCCATTCCGTCCCTCATCAGACTCGTCAACATATGTTTCTTGACTACATATCTTTCCATATACCAGATGAGTCCTTCTCCGGTATGGAAAATTATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas

Protein Analysis

255

Amino Acids

29.15

Weight (kDa)

4.7

Isoelectric Point (pI)

42.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANAPC4_WD40 PF12894 40 - 78 3.6e-08 Anaphase-promoting complex subunit 4 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 486
AasI GACNNNNNNGTC 1 cut(s) 59
Acc36I ACCTGC 1 cut(s) 486
AccI GTMKAC 1 cut(s) 626
AccII CGCG 2 cut(s) 27, 83
AciI CCGC 2 cut(s) 83, 590
AclWI GGATC 1 cut(s) 353
AcsI RAATTY 2 cut(s) 230, 500
AcyI GRCGYC 1 cut(s) 383
AfaI GTAC 1 cut(s) 311
AflIII ACRYGT 1 cut(s) 25
AgsI TTSAA 6 cut(s) 230, 247, 259, 340, 506, 561
AjnI CCWGG 1 cut(s) 136
AluBI AGCT 2 cut(s) 43, 392
AluI AGCT 2 cut(s) 43, 392
Alw21I GWGCWC 1 cut(s) 477
Alw26I GTCTC 1 cut(s) 160
AlwI GGATC 1 cut(s) 353
Ama87I CYCGRG 1 cut(s) 95
ApoI RAATTY 2 cut(s) 230, 500
Asp700I GAANNNNTTC 2 cut(s) 242, 444
AsuII TTCGAA 1 cut(s) 236
AvaI CYCGRG 1 cut(s) 95
BanII GRGCYC 1 cut(s) 198
Bbv12I GWGCWC 1 cut(s) 477
BccI CCATC 3 cut(s) 167, 197, 505
BceAI ACGGC 1 cut(s) 173
BciT130I CCWGG 1 cut(s) 138
BclI TGATCA 1 cut(s) 292
BcoDI GTCTC 1 cut(s) 160
BfaI CTAG 1 cut(s) 519
BfmI CTRYAG 1 cut(s) 479
BfuAI ACCTGC 1 cut(s) 486
BisI GCNGC 1 cut(s) 590
BlsI GCNGC 1 cut(s) 591
Bme1390I CCNGG 1 cut(s) 138
BmeT110I CYCGRG 1 cut(s) 95
BmrFI CCNGG 1 cut(s) 138
BmrI ACTGGG 1 cut(s) 282
BmsI GCATC 3 cut(s) 531, 626, 652
BmuI ACTGGG 1 cut(s) 282
BplI GAGNNNNNCTC 4 cut(s) 650, 682, 728, 760
BpmI CTGGAG 2 cut(s) 29, 398
Bpu14I TTCGAA 1 cut(s) 236
BsaBI GATNNNNATC 2 cut(s) 77, 297
BsaHI GRCGYC 1 cut(s) 383
BsaI GGTCTC 1 cut(s) 160
BsaJI CCNNGG 3 cut(s) 136, 137, 495
BsaWI WCCGGW 1 cut(s) 746
Bse1I ACTGG 4 cut(s) 46, 59, 288, 630
Bse3DI GCAATG 2 cut(s) 215, 584
Bse8I GATNNNNATC 2 cut(s) 77, 297
BseBI CCWGG 1 cut(s) 138
BseDI CCNNGG 3 cut(s) 136, 137, 495
BseGI GGATG 1 cut(s) 667
BseJI GATNNNNATC 2 cut(s) 77, 297
BseMI GCAATG 2 cut(s) 215, 584
BseNI ACTGG 4 cut(s) 46, 59, 288, 630
BsgI GTGCAG 1 cut(s) 446
Bsh1236I CGCG 2 cut(s) 27, 83
BsiHKAI GWGCWC 1 cut(s) 477
BsiHKCI CYCGRG 1 cut(s) 95
BsiSI CCGG 1 cut(s) 747
BslFI GGGAC 1 cut(s) 662
BsmAI GTCTC 1 cut(s) 160
BsmFI GGGAC 1 cut(s) 662
Bso31I GGTCTC 1 cut(s) 160
BsoBI CYCGRG 1 cut(s) 95
Bsp119I TTCGAA 1 cut(s) 236
Bsp1286I GDGCHC 2 cut(s) 198, 477
Bsp1407I TGTACA 1 cut(s) 309
Bsp143I GATC 3 cut(s) 78, 292, 358
BspACI CCGC 2 cut(s) 83, 590
BspFNI CGCG 2 cut(s) 27, 83
BspMAI CTGCAG 1 cut(s) 483
BspMI ACCTGC 1 cut(s) 486
BspPI GGATC 1 cut(s) 353
BspQI GCTCTTC 1 cut(s) 137
BspT104I TTCGAA 1 cut(s) 236
BspTNI GGTCTC 1 cut(s) 160
BsrDI GCAATG 2 cut(s) 215, 584
BsrGI TGTACA 1 cut(s) 309
BsrI ACTGG 4 cut(s) 46, 59, 288, 630
BssECI CCNNGG 3 cut(s) 136, 137, 495
BssMI GATC 3 cut(s) 78, 292, 358
BssNI GRCGYC 1 cut(s) 383
BssT1I CCWWGG 1 cut(s) 495
Bst2UI CCWGG 1 cut(s) 138
Bst4CI ACNGT 2 cut(s) 309, 424
Bst6I CTCTTC 1 cut(s) 137
BstACI GRCGYC 1 cut(s) 383
BstAUI TGTACA 1 cut(s) 309
BstBI TTCGAA 1 cut(s) 236
BstF5I GGATG 1 cut(s) 667
BstFNI CGCG 2 cut(s) 27, 83
BstKTI GATC 3 cut(s) 81, 295, 361
BstMAI GTCTC 1 cut(s) 160
BstMBI GATC 3 cut(s) 78, 292, 358
BstMWI GCNNNNNNNGC 1 cut(s) 389
BstNI CCWGG 1 cut(s) 138
BstSCI CCNGG 1 cut(s) 136
BstSFI CTRYAG 1 cut(s) 479
BstUI CGCG 2 cut(s) 27, 83
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
BtsCI GGATG 1 cut(s) 667
BtsIMutI CAGTG 2 cut(s) 295, 429
BveI ACCTGC 1 cut(s) 486
CseI GACGC 2 cut(s) 16, 372
Csp6I GTAC 1 cut(s) 310
CviAII CATG 3 cut(s) 115, 221, 765
CviJI RGCY 8 cut(s) 43, 58, 121, 196, 253, 392, 494, 533
CviKI_1 RGCY 8 cut(s) 43, 58, 121, 196, 253, 392, 494, 533
CviQI GTAC 1 cut(s) 310
DpnI GATC 3 cut(s) 80, 294, 360
DpnII GATC 3 cut(s) 78, 292, 358
DrdI GACNNNNNNGTC 1 cut(s) 59
DseDI GACNNNNNNGTC 1 cut(s) 59
Eam1104I CTCTTC 1 cut(s) 137
EarI CTCTTC 1 cut(s) 137
Eco130I CCWWGG 1 cut(s) 495
Eco24I GRGCYC 1 cut(s) 198
Eco31I GGTCTC 1 cut(s) 160
Eco88I CYCGRG 1 cut(s) 95
EcoRII CCWGG 1 cut(s) 136
EcoT14I CCWWGG 1 cut(s) 495
EcoT22I ATGCAT 1 cut(s) 766
EcoT38I GRGCYC 1 cut(s) 198
ErhI CCWWGG 1 cut(s) 495
FaeI CATG 3 cut(s) 118, 224, 768
FaqI GGGAC 1 cut(s) 662
FatI CATG 3 cut(s) 114, 220, 764
FauNDI CATATG 1 cut(s) 699
FbaI TGATCA 1 cut(s) 292
FblI GTMKAC 1 cut(s) 626
Fnu4HI GCNGC 1 cut(s) 590
FokI GGATG 1 cut(s) 674
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 1 cut(s) 590
FspBI CTAG 1 cut(s) 519
GluI GCNGC 1 cut(s) 590
GsuI CTGGAG 2 cut(s) 29, 398
HapII CCGG 1 cut(s) 747
HgaI GACGC 2 cut(s) 16, 372
Hin1I GRCGYC 1 cut(s) 383
Hin1II CATG 3 cut(s) 118, 224, 768
HincII GTYRAC 1 cut(s) 695
HindII GTYRAC 1 cut(s) 695
HinfI GANTC 5 cut(s) 91, 515, 581, 688, 737
HpaII CCGG 1 cut(s) 747
Hpy166II GTNNAC 3 cut(s) 320, 627, 695
Hpy188I TCNGA 4 cut(s) 283, 388, 466, 687
Hpy188III TCNNGA 7 cut(s) 95, 124, 256, 337, 538, 561, 707
Hpy8I GTNNAC 3 cut(s) 320, 627, 695
Hpy99I CGWCG 3 cut(s) 14, 194, 388
HpyAV CCTTC 2 cut(s) 94, 751
HpyCH4III ACNGT 2 cut(s) 309, 424
HpyCH4IV ACGT 2 cut(s) 152, 488
HpyCH4V TGCA 4 cut(s) 220, 427, 481, 764
HpyF10VI GCNNNNNNNGC 1 cut(s) 389
HpySE526I ACGT 2 cut(s) 152, 488
Hsp92I GRCGYC 1 cut(s) 383
Hsp92II CATG 3 cut(s) 118, 224, 768
Ksp22I TGATCA 1 cut(s) 292
Kzo9I GATC 3 cut(s) 78, 292, 358
LguI GCTCTTC 1 cut(s) 137
LmnI GCTCC 2 cut(s) 48, 206
LweI GCATC 3 cut(s) 531, 626, 652
MaeI CTAG 1 cut(s) 519
MaeII ACGT 2 cut(s) 152, 488
MaeIII GTNAC 2 cut(s) 28, 270
MalI GATC 3 cut(s) 80, 294, 360
MboI GATC 3 cut(s) 78, 292, 358
MboII GAAGA 7 cut(s) 26, 29, 154, 212, 235, 293, 590
MfeI CAATTG 2 cut(s) 247, 371
MflI RGATCY 1 cut(s) 358
MhlI GDGCHC 2 cut(s) 198, 477
MluCI AATT 8 cut(s) 230, 247, 277, 371, 440, 500, 555, 757
MluI ACGCGT 1 cut(s) 25
MlyI GAGTC 3 cut(s) 524, 682, 746
MmeI TCCRAC 3 cut(s) 143, 366, 391
MnlI CCTC 7 cut(s) 111, 373, 477, 523, 652, 676, 690
Mph1103I ATGCAT 1 cut(s) 766
MroXI GAANNNNTTC 2 cut(s) 242, 444
MseI TTAA 3 cut(s) 75, 366, 525
MspI CCGG 1 cut(s) 747
MspR9I CCNGG 1 cut(s) 138
MunI CAATTG 2 cut(s) 247, 371
MvaI CCWGG 1 cut(s) 138
MvnI CGCG 2 cut(s) 27, 83
MwoI GCNNNNNNNGC 1 cut(s) 389
NdeI CATATG 1 cut(s) 699
NdeII GATC 3 cut(s) 78, 292, 358
NlaIII CATG 3 cut(s) 118, 224, 768
NmuCI GTSAC 1 cut(s) 28
NsiI ATGCAT 1 cut(s) 766
NspV TTCGAA 1 cut(s) 236
PaqCI CACCTGC 1 cut(s) 486
PasI CCCWGGG 1 cut(s) 137
PciSI GCTCTTC 1 cut(s) 137
PdmI GAANNNNTTC 2 cut(s) 242, 444
PfeI GAWTC 2 cut(s) 91, 581
PkrI GCNGC 1 cut(s) 591
PleI GAGTC 3 cut(s) 523, 682, 745
PpsI GAGTC 3 cut(s) 523, 682, 745
Psp6I CCWGG 1 cut(s) 136
PspGI CCWGG 1 cut(s) 136
PstI CTGCAG 1 cut(s) 483
PsuI RGATCY 1 cut(s) 358
RsaI GTAC 1 cut(s) 311
RsaNI GTAC 1 cut(s) 310
SapI GCTCTTC 1 cut(s) 137
SaqAI TTAA 3 cut(s) 75, 366, 525
SatI GCNGC 1 cut(s) 590
Sau3AI GATC 3 cut(s) 78, 292, 358
SchI GAGTC 3 cut(s) 524, 682, 746
ScrFI CCNGG 1 cut(s) 138
SduI GDGCHC 2 cut(s) 198, 477
SetI ASST 7 cut(s) 45, 155, 172, 394, 480, 491, 610
SfaNI GCATC 3 cut(s) 531, 626, 652
SfcI CTRYAG 1 cut(s) 479
SfuI TTCGAA 1 cut(s) 236
Sse9I AATT 8 cut(s) 230, 247, 277, 371, 440, 500, 555, 757
SsiI CCGC 2 cut(s) 83, 590
SspMI CTAG 1 cut(s) 519
StyD4I CCNGG 1 cut(s) 136
StyI CCWWGG 1 cut(s) 495
TaaI ACNGT 2 cut(s) 309, 424
TaiI ACGT 2 cut(s) 155, 491
TaqI TCGA 3 cut(s) 51, 198, 236
TasI AATT 8 cut(s) 230, 247, 277, 371, 440, 500, 555, 757
TatI WGTACW 1 cut(s) 309
TauI GCSGC 1 cut(s) 592
TfiI GAWTC 2 cut(s) 91, 581
Tru1I TTAA 3 cut(s) 75, 366, 525
Tru9I TTAA 3 cut(s) 75, 366, 525
TscAI CASTG 2 cut(s) 295, 429
TseFI GTSAC 1 cut(s) 28
Tsp45I GTSAC 1 cut(s) 28
TspDTI ATGAA 2 cut(s) 204, 573
TspGWI ACGGA 1 cut(s) 664
TspRI CASTG 2 cut(s) 295, 429
XapI RAATTY 2 cut(s) 230, 500
XmiI GTMKAC 1 cut(s) 626
XmnI GAANNNNTTC 2 cut(s) 242, 444
XspI CTAG 1 cut(s) 519
Zsp2I ATGCAT 1 cut(s) 766
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.