Rh5AG414600

TPL-binding domain in jasmonate signalling

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0007983
Physical Location & Seq
Forward (+)
71618079 .. 71620792
2714 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG414600.1

Sequence Viewer

Length: 1401 bp
ATGTCGTTTCAGAACAAGGGTTTTTGGATGGCAAAGGGTGCGGGACATGAAAATGATCGAGATGCAACTTTTGGTAGCCCTTCAAGAATTGAACCAAAGCGGTCTCACCAATGGTTTGTAGATTCTGCTGAACCCCAGATGTTCCCCAACAAAAAGCAAGCAGTACATATTCCAAACAGCAAACTGAGCACGGATATGCCCAATGAAAATGTCTCTTGGGAGAATCCTTCCAGCTTTCAGTCGGTTCCCCATCAATTTATTGACCGGTTATTTGGATCGGATACAGCAAGCTCTGCCAACTTTTCAGACAGGAACGTGTCTCCTGTTGGATCAGATGATTGGAGCACAAGAACAAAGGGTATTGATGACCAGTTCGGAGAAGATGCTGCGGTAAATTTGTCCATATCTCATGCTATTGAAGATCCTGAAGTATGTCTTGGCTTTACTGGGATTAGGAAAATCAAAGTCAACCAGGTTAAGGACAGCGACAATGGCATGCATGCATCAAGGGAACATGGTTCTAGCAGGGAATATAACAGTAACCTGCCCACAAGTCAGGCATTTGATAGGACACACGAAACCAGTTTCATATCAGCAGGGCAGGCATATGATAAGGATGTCACACTAATGGGTCATGCCTACAACCAGGGAGCTGCCCATGTTAGACCTTTAGGTCCCAACTATGGCAAGAGGGAAGAAAATGTGATATCAATGGGTGATGGTTACAGTAAAGGTAATGCCAATATGATATCTTTTGGCGGGTTTCCTGACGAACAAGATATGAACACCATGGGCAGGGCTGTTGCAAACTATGATCAATTATACCATCAGTCTTCTGTACAAACATCAGAAACTGTGAATGAGAAAGAATTGGATGCAACAAATGTCAATGCAGTTGATAATACTGCTTCTGTAGCTAAATCAAGACCAGAATCAGCTTCCAAGAGTAAACCAGAGATCAAACCAATAAAGAAACAAGCTCCAAACAGCTTCCCCTCTAATGTTAGAAGCTTGATTTCAACTGGCATCTTAGATGGCGTTCCTGTAAAGTATGTCTCCATGGCACGGGAGGAACTTCGCGGAATTATAAAGGGTTCCAGCTATCTTTGCGGCTGTCAAGCATGTAACTTTACCAAGGGGCTAAATGCTTATGAATTTGAGCGGCATGCTGGTTGCAAAACAAAACATCCAAACAATCACATATACTTTGAGAATGGGAAGACCATCTATCAGATAGTACAGGAATTAAGGAGCACCCCTGAAAGCTTGTTATTCGATACAATGCAAACAGTCTTTGGCGCTCCGATCAATCAGAAAGCTTTTCTCAGTTGGAAAGAATCCTTTCAAGCAGCAACTCGTGAACTTCAGCGCATTTACGGAAAGGAGGAACTGAACTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Jas

Protein Analysis

466

Amino Acids

51.71

Weight (kDa)

6.18

Isoelectric Point (pI)

41.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TDBD PF16135 361 - 416 3.3e-13 Tify domain binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1088
AasI GACNNNNNNGTC 1 cut(s) 672
Acc36I ACCTGC 1 cut(s) 552
AccBSI CCGCTC 1 cut(s) 1162
AccII CGCG 1 cut(s) 1080
AciI CCGC 7 cut(s) 41, 100, 389, 759, 1080, 1110, 1162
AclWI GGATC 3 cut(s) 283, 337, 416
AcsI RAATTY 2 cut(s) 394, 1154
AcuI CTGAAG 2 cut(s) 447, 1349
AfaI GTAC 3 cut(s) 165, 840, 1239
AfiI CCNNNNNNNGG 4 cut(s) 478, 683, 795, 1065
AflIII ACRYGT 1 cut(s) 315
AgeI ACCGGT 1 cut(s) 264
AgsI TTSAA 5 cut(s) 84, 92, 419, 1020, 1346
AjnI CCWGG 2 cut(s) 471, 645
AjuI GAANNNNNNNTTGG 2 cut(s) 420, 452
Alw21I GWGCWC 3 cut(s) 191, 347, 1256
Alw26I GTCTC 4 cut(s) 108, 217, 324, 1060
AlwI GGATC 3 cut(s) 283, 337, 416
AlwNI CAGNNNCTG 1 cut(s) 854
ApeKI GCWGC 3 cut(s) 386, 653, 1349
ApoI RAATTY 2 cut(s) 394, 1154
ArsI GACNNNNNNTTYG 2 cut(s) 254, 286
AsiGI ACCGGT 1 cut(s) 264
Asp700I GAANNNNTTC 1 cut(s) 1341
AspLEI GCGC 2 cut(s) 1301, 1371
AspS9I GGNCC 1 cut(s) 674
AsuHPI GGTGA 2 cut(s) 98, 728
AvaII GGWCC 1 cut(s) 674
BauI CACGAG 1 cut(s) 1356
BbsI GAAGAC 2 cut(s) 825, 1226
Bbv12I GWGCWC 3 cut(s) 191, 347, 1256
BbvI GCAGC 3 cut(s) 373, 640, 1361
BccI CCATC 6 cut(s) 22, 258, 713, 834, 1028, 1232
BciT130I CCWGG 2 cut(s) 473, 647
BciVI GTATCC 1 cut(s) 274
BclI TGATCA 1 cut(s) 814
BcoDI GTCTC 4 cut(s) 108, 217, 324, 1060
BfaI CTAG 1 cut(s) 522
BfmI CTRYAG 1 cut(s) 912
BfoI RGCGCY 1 cut(s) 1302
BfuAI ACCTGC 1 cut(s) 552
BfuI GTATCC 1 cut(s) 274
BisI GCNGC 5 cut(s) 387, 654, 1111, 1163, 1350
BlsI GCNGC 5 cut(s) 388, 655, 1112, 1164, 1351
Bme1390I CCNGG 2 cut(s) 473, 647
Bme18I GGWCC 1 cut(s) 674
BmgT120I GGNCC 1 cut(s) 674
BmiI GGNNCC 3 cut(s) 246, 676, 1096
BmrFI CCNGG 2 cut(s) 473, 647
BmrI ACTGGG 1 cut(s) 456
BmsI GCATC 5 cut(s) 52, 373, 512, 865, 1035
BmuI ACTGGG 1 cut(s) 456
BpiI GAAGAC 2 cut(s) 825, 1226
BsaI GGTCTC 1 cut(s) 108
BsaJI CCNNGG 4 cut(s) 646, 789, 1059, 1134
BsaWI WCCGGW 1 cut(s) 264
Bsc4I CCNNNNNNNGG 4 cut(s) 478, 683, 795, 1065
Bse118I RCCGGY 1 cut(s) 264
Bse1I ACTGG 4 cut(s) 370, 451, 582, 1027
BseBI CCWGG 2 cut(s) 473, 647
BseDI CCNNGG 4 cut(s) 646, 789, 1059, 1134
BseGI GGATG 4 cut(s) 33, 622, 880, 1186
BseLI CCNNNNNNNGG 4 cut(s) 478, 683, 795, 1065
BseMII CTCAG 2 cut(s) 176, 1339
BseNI ACTGG 4 cut(s) 370, 451, 582, 1027
BseXI GCAGC 3 cut(s) 373, 640, 1361
Bsh1236I CGCG 1 cut(s) 1080
BshTI ACCGGT 1 cut(s) 264
BsiHKAI GWGCWC 3 cut(s) 191, 347, 1256
BsiSI CCGG 1 cut(s) 265
BslFI GGGAC 2 cut(s) 57, 660
BslI CCNNNNNNNGG 4 cut(s) 478, 683, 795, 1065
BsmAI GTCTC 4 cut(s) 108, 217, 324, 1060
BsmFI GGGAC 2 cut(s) 57, 660
Bso31I GGTCTC 1 cut(s) 108
Bsp1286I GDGCHC 3 cut(s) 191, 347, 1256
Bsp1407I TGTACA 1 cut(s) 838
Bsp143I GATC 7 cut(s) 55, 275, 329, 421, 814, 957, 1305
Bsp19I CCATGG 2 cut(s) 789, 1059
BspACI CCGC 7 cut(s) 41, 100, 389, 759, 1080, 1110, 1162
BspCNI CTCAG 2 cut(s) 177, 1338
BspFNI CGCG 1 cut(s) 1080
BspLI GGNNCC 3 cut(s) 246, 676, 1096
BspMI ACCTGC 1 cut(s) 552
BspPI GGATC 3 cut(s) 283, 337, 416
BspTNI GGTCTC 1 cut(s) 108
BsrBI CCGCTC 1 cut(s) 1162
BsrFI RCCGGY 1 cut(s) 264
BsrGI TGTACA 1 cut(s) 838
BsrI ACTGG 4 cut(s) 370, 451, 582, 1027
BssAI RCCGGY 1 cut(s) 264
BssECI CCNNGG 4 cut(s) 646, 789, 1059, 1134
BssMI GATC 7 cut(s) 55, 275, 329, 421, 814, 957, 1305
BssSI CACGAG 1 cut(s) 1356
BssT1I CCWWGG 3 cut(s) 789, 1059, 1134
Bst2BI CACGAG 1 cut(s) 1356
Bst2UI CCWGG 2 cut(s) 473, 647
Bst4CI ACNGT 4 cut(s) 539, 728, 856, 1291
BstAPI GCANNNNNTGC 2 cut(s) 38, 293
BstAUI TGTACA 1 cut(s) 838
BstC8I GCNNGC 6 cut(s) 159, 289, 497, 501, 603, 1167
BstDEI CTNAG 3 cut(s) 185, 1030, 1325
BstDSI CCRYGG 2 cut(s) 789, 1059
BstF5I GGATG 4 cut(s) 33, 622, 880, 1186
BstFNI CGCG 1 cut(s) 1080
BstH2I RGCGCY 1 cut(s) 1302
BstHHI GCGC 2 cut(s) 1301, 1371
BstKTI GATC 7 cut(s) 58, 278, 332, 424, 817, 960, 1308
BstMAI GTCTC 4 cut(s) 108, 217, 324, 1060
BstMBI GATC 7 cut(s) 55, 275, 329, 421, 814, 957, 1305
BstMWI GCNNNNNNNGC 7 cut(s) 38, 186, 293, 492, 602, 914, 1107
BstNI CCWGG 2 cut(s) 473, 647
BstNSI RCATGY 4 cut(s) 499, 503, 1125, 1169
BstSCI CCNGG 2 cut(s) 471, 645
BstSFI CTRYAG 1 cut(s) 912
BstUI CGCG 1 cut(s) 1080
BstV1I GCAGC 3 cut(s) 373, 640, 1361
BstV2I GAAGAC 2 cut(s) 825, 1226
BstX2I RGATCY 1 cut(s) 421
BstYI RGATCY 1 cut(s) 421
BsuI GTATCC 1 cut(s) 274
BtgI CCRYGG 2 cut(s) 789, 1059
BtsCI GGATG 4 cut(s) 33, 622, 880, 1186
BveI ACCTGC 1 cut(s) 552
Cac8I GCNNGC 6 cut(s) 159, 289, 497, 501, 603, 1167
CaiI CAGNNNCTG 1 cut(s) 854
CfoI GCGC 2 cut(s) 1301, 1371
Cfr10I RCCGGY 1 cut(s) 264
Cfr13I GGNCC 1 cut(s) 674
CsiI ACCWGGT 1 cut(s) 471
Csp6I GTAC 3 cut(s) 164, 839, 1238
CspAI ACCGGT 1 cut(s) 264
CviQI GTAC 3 cut(s) 164, 839, 1238
DdeI CTNAG 3 cut(s) 185, 1030, 1325
DpnI GATC 7 cut(s) 57, 277, 331, 423, 816, 959, 1307
DpnII GATC 7 cut(s) 55, 275, 329, 421, 814, 957, 1305
DrdI GACNNNNNNGTC 1 cut(s) 672
DseDI GACNNNNNNGTC 1 cut(s) 672
Eco130I CCWWGG 3 cut(s) 789, 1059, 1134
Eco31I GGTCTC 1 cut(s) 108
Eco32I GATATC 2 cut(s) 708, 750
Eco47I GGWCC 1 cut(s) 674
Eco57I CTGAAG 2 cut(s) 447, 1349
EcoO109I RGGNCCY 1 cut(s) 674
EcoRII CCWGG 2 cut(s) 471, 645
EcoRV GATATC 2 cut(s) 708, 750
EcoT14I CCWWGG 3 cut(s) 789, 1059, 1134
EcoT22I ATGCAT 2 cut(s) 501, 505
ErhI CCWWGG 3 cut(s) 789, 1059, 1134
FalI AAGNNNNNCTT 2 cut(s) 420, 452
FaqI GGGAC 2 cut(s) 57, 660
FauI CCCGC 2 cut(s) 34, 752
FauNDI CATATG 1 cut(s) 607
FbaI TGATCA 1 cut(s) 814
Fnu4HI GCNGC 5 cut(s) 387, 654, 1111, 1163, 1350
FokI GGATG 4 cut(s) 40, 629, 887, 1173
Fsp4HI GCNGC 5 cut(s) 387, 654, 1111, 1163, 1350
FspBI CTAG 1 cut(s) 522
GlaI GCGC 2 cut(s) 1300, 1370
GluI GCNGC 5 cut(s) 387, 654, 1111, 1163, 1350
HaeII RGCGCY 1 cut(s) 1302
HapII CCGG 1 cut(s) 265
HhaI GCGC 2 cut(s) 1301, 1371
Hin6I GCGC 2 cut(s) 1299, 1369
HinP1I GCGC 2 cut(s) 1299, 1369
HincII GTYRAC 1 cut(s) 469
HindII GTYRAC 1 cut(s) 469
HindIII AAGCTT 3 cut(s) 1009, 1264, 1317
HinfI GANTC 4 cut(s) 122, 223, 932, 1337
HpaII CCGG 1 cut(s) 265
HphI GGTGA 2 cut(s) 98, 728
Hpy166II GTNNAC 3 cut(s) 469, 950, 1361
Hpy188I TCNGA 9 cut(s) 12, 280, 307, 334, 377, 850, 1233, 1305, 1314
Hpy188III TCNNGA 6 cut(s) 59, 84, 425, 767, 924, 1358
Hpy8I GTNNAC 3 cut(s) 469, 950, 1361
HpyAV CCTTC 2 cut(s) 90, 237
HpyCH4III ACNGT 4 cut(s) 539, 728, 856, 1291
HpyCH4IV ACGT 1 cut(s) 315
HpyCH4V TGCA 8 cut(s) 65, 499, 503, 806, 878, 893, 1176, 1285
HpyF10VI GCNNNNNNNGC 7 cut(s) 38, 186, 293, 492, 602, 914, 1107
HpyF3I CTNAG 3 cut(s) 185, 1030, 1325
HpySE526I ACGT 1 cut(s) 315
HspAI GCGC 2 cut(s) 1299, 1369
Ksp22I TGATCA 1 cut(s) 814
Kzo9I GATC 7 cut(s) 55, 275, 329, 421, 814, 957, 1305
LmnI GCTCC 5 cut(s) 342, 650, 985, 1251, 1306
Lsp1109I GCAGC 3 cut(s) 373, 640, 1361
LweI GCATC 5 cut(s) 52, 373, 512, 865, 1035
MabI ACCWGGT 1 cut(s) 471
MaeI CTAG 1 cut(s) 522
MaeII ACGT 1 cut(s) 315
MaeIII GTNAC 4 cut(s) 539, 619, 722, 1124
MalI GATC 7 cut(s) 57, 277, 331, 423, 816, 959, 1307
MbiI CCGCTC 1 cut(s) 1162
MboI GATC 7 cut(s) 55, 275, 329, 421, 814, 957, 1305
MboII GAAGA 5 cut(s) 392, 431, 707, 825, 1231
MflI RGATCY 1 cut(s) 421
MhlI GDGCHC 3 cut(s) 191, 347, 1256
MluCI AATT 8 cut(s) 87, 254, 394, 818, 869, 1083, 1154, 1244
MmeI TCCRAC 2 cut(s) 307, 1310
MnlI CCTC 4 cut(s) 684, 1006, 1063, 1378
Mph1103I ATGCAT 2 cut(s) 501, 505
MroXI GAANNNNTTC 1 cut(s) 1341
MseI TTAA 2 cut(s) 477, 1247
MslI CAYNNNNRTG 3 cut(s) 51, 194, 626
MspI CCGG 1 cut(s) 265
MspR9I CCNGG 2 cut(s) 473, 647
MvaI CCWGG 2 cut(s) 473, 647
MvnI CGCG 1 cut(s) 1080
MwoI GCNNNNNNNGC 7 cut(s) 38, 186, 293, 492, 602, 914, 1107
NcoI CCATGG 2 cut(s) 789, 1059
NdeI CATATG 1 cut(s) 607
NdeII GATC 7 cut(s) 55, 275, 329, 421, 814, 957, 1305
NlaIV GGNNCC 3 cut(s) 246, 676, 1096
NmuCI GTSAC 1 cut(s) 619
NsiI ATGCAT 2 cut(s) 501, 505
NspI RCATGY 4 cut(s) 499, 503, 1125, 1169
PaeI GCATGC 3 cut(s) 499, 503, 1169
PdmI GAANNNNTTC 1 cut(s) 1341
PfeI GAWTC 4 cut(s) 122, 223, 932, 1337
PinAI ACCGGT 1 cut(s) 264
PkrI GCNGC 5 cut(s) 388, 655, 1112, 1164, 1351
PpuMI RGGWCCY 1 cut(s) 674
PsiI TTATAA 1 cut(s) 1088
Psp5II RGGWCCY 1 cut(s) 674
Psp6I CCWGG 2 cut(s) 471, 645
PspGI CCWGG 2 cut(s) 471, 645
PspN4I GGNNCC 3 cut(s) 246, 676, 1096
PspPI GGNCC 1 cut(s) 674
PspPPI RGGWCCY 1 cut(s) 674
PstNI CAGNNNCTG 1 cut(s) 854
PsuI RGATCY 1 cut(s) 421
RsaI GTAC 3 cut(s) 165, 840, 1239
RsaNI GTAC 3 cut(s) 164, 839, 1238
RseI CAYNNNNRTG 3 cut(s) 51, 194, 626
SaqAI TTAA 2 cut(s) 477, 1247
SatI GCNGC 5 cut(s) 387, 654, 1111, 1163, 1350
Sau3AI GATC 7 cut(s) 55, 275, 329, 421, 814, 957, 1305
Sau96I GGNCC 1 cut(s) 674
ScrFI CCNGG 2 cut(s) 473, 647
SduI GDGCHC 3 cut(s) 191, 347, 1256
SexAI ACCWGGT 1 cut(s) 471
SfaNI GCATC 5 cut(s) 52, 373, 512, 865, 1035
SfcI CTRYAG 1 cut(s) 912
SinI GGWCC 1 cut(s) 674
SmiMI CAYNNNNRTG 3 cut(s) 51, 194, 626
SphI GCATGC 3 cut(s) 499, 503, 1169
Sse9I AATT 8 cut(s) 87, 254, 394, 818, 869, 1083, 1154, 1244
SsiI CCGC 7 cut(s) 41, 100, 389, 759, 1080, 1110, 1162
SspMI CTAG 1 cut(s) 522
StyD4I CCNGG 2 cut(s) 471, 645
StyI CCWWGG 3 cut(s) 789, 1059, 1134
TaaI ACNGT 4 cut(s) 539, 728, 856, 1291
TaiI ACGT 1 cut(s) 318
TaqI TCGA 2 cut(s) 58, 1275
TasI AATT 8 cut(s) 87, 254, 394, 818, 869, 1083, 1154, 1244
TatI WGTACW 3 cut(s) 163, 838, 1237
TauI GCSGC 2 cut(s) 1113, 1165
TfiI GAWTC 4 cut(s) 122, 223, 932, 1337
Tru1I TTAA 2 cut(s) 477, 1247
Tru9I TTAA 2 cut(s) 477, 1247
TseFI GTSAC 1 cut(s) 619
TseI GCWGC 3 cut(s) 386, 653, 1349
Tsp45I GTSAC 1 cut(s) 619
TspDTI ATGAA 5 cut(s) 63, 219, 577, 797, 1167
TspGWI ACGGA 2 cut(s) 206, 1392
VpaK11BI GGWCC 1 cut(s) 674
XapI RAATTY 2 cut(s) 394, 1154
XceI RCATGY 4 cut(s) 499, 503, 1125, 1169
XmnI GAANNNNTTC 1 cut(s) 1341
XspI CTAG 1 cut(s) 522
Zsp2I ATGCAT 2 cut(s) 501, 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.