MD03G1114500.v1.1

WD repeat-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007846
Physical Location & Seq
Reverse (-)
10003875 .. 10008069
4195 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1114500.v1.1.491

Sequence Viewer

Length: 1824 bp
ATGTTCAACAACGGGTACGCCAACAACAAAAACTGCCTGAGCCCGATGCCGTCGGCATCATCGTCAACGACGGCGACGCCCCAAACGACGTCGTCTTCTTCTTCTGGCACAACAAATTCCCAGACCCAATCTCTGATAAAGGCGTATTTCAAAACCCCAGAAGGCCGATACAAACTTCACTCGGAGAAGACTCGGCCGCCCAACCTCCTCCCCTACTCGTACGCCAAGTCAATTTCTCAGGTGACGGTAGCGCAGCTCAAGGACAAGCCTAGCAGCCAGCCTACGCTGACGCCGAGTTTCAGCACCACCGGGGTGCGGTCGGCGGCGGCGAGGCTGTTGGGAGGAAGCGGGGGCAAAGCTCTAAACTTTGTGGGAGGGAGTGGATATAGCAGAACCAGCAATGGGAGTAGCAGGTATGCTCATTTCGGCGGGTCGAATGGGACCCACCACGCTGTCAACTCGAATCTCGGCGGTAAAGGGTCGTTTTTGGTTTTCAATGTTGGGGATACTGTTTATATCAGTGATCTCAATTCGCCTGATAAGGATCCTATTAAGTCCTTGCATTTTAGCAATTCGAACCCAGTGTGCCATGCTTTTGATTCCGAGGCCAAGGACGGGCATGACTTGCTTATTGGATTGAGCTCTGGAGATATTTATTCCGCGTCTTTGAGGCAGCAATTGCAAGATTCTGGAAAGAAGCTTGTTGGTGCCCATCATTATAACAAAGAAGGTGCTGTCACTAGCAGCCGGTGTACCAGTGTTGCGTGGGTTCCTAAAGGGAATGGTACCTGTGTTGCTGCTCATGCTGATGGAAATTTGTATAGTAAGGAAAGTGCTGGTGATTCTTCTTTCCCAGCTATAAAGGATCATACTCAATTTTCGGTGGCTCATGCAAGATCCAGTAAGAGTAATCCAATTTCCAGATGGCATATTTGCCAAGGTTCAATAAACAGCATTGTTTACTCAGCAGATGGGGAAAATTTGGCAACCGTCGGTAGAGATGGCTATTTGCGAGTATTTGACTACTCAAAAGAACAACTAGTATTCGGTGGTAAGAGTTATTATGGTGCTCTTCTGTGTTGCTCGTGGAGTCCGGATGGGAAATACATTTTGACAGGAGGGGAAGAGGATCTTGTTACCGTGTGGAGCATGGAAGATAGAAAAGTAGTAGCTTGGGGTGAAGGGCACAACTCATGGGTCAGTGGTGTTGCTTTTGATTCATATTGGTCACCACCAAGTTCAAATGACACGGAAGAAAATATTGTTTACCGTTTTGGTTCTGTGGGTCAGGACACAAAATTGCTCCTATGGGACCTGCCAATGGATGAGATTGTAGTGCCGCTTCGCCGAAGTTCGCTTGGTGGATCTCCAACATTCAGCACAGGTAGCCAGTCATCCCATTGGGACAGCGTTTACCCTATTGGCACTCTTCAACCTGCTCCCAGCATGCGGGACATTCCAAAGCTCTCCCCACTGGTGTCCCACTGCGTTCATTCTGAGCCCCTATCCGGATTACTTTTCACAGAGGATGCTATTCTCACCGCATCTTGGGAGGGGCATGTGAAAATTTGGATGAGACCCGGTGAGCCTGAAACTAAAACAAACGCAGAAGCCCTGCTAGGTGCTAGTCCGAAGGACCAACTCCTCACAGGCAAAGGTTACACAGAACTGGCAAAAAGCAAGCCATTCACTGAAAGTAAATCATACAACCCACAATCCCTGCTCGGCGCTACTTCAAAGGAGCAACAACCACTGACAGGCAAAGGTGGCGCTTTTAATTTCAAGGAAATGTCGAAAAGCAGTCGATTGGGGTACCTTCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

608

Amino Acids

65.12

Weight (kDa)

8.4

Isoelectric Point (pI)

44.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_1st PF25173 302 - 443 7.2e-10 WDR3 first beta-propeller domain
WDR55 PF24796 303 - 385 8.2e-06 WDR55
WD40_Prp19 PF24814 306 - 405 5.1e-08 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 310 - 405 1e-10 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 312 - 404 9.3e-06 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 315 - 406 1.9e-11 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 325 - 406 3.2e-07 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 720
AatII GACGTC 1 cut(s) 92
Acc36I ACCTGC 3 cut(s) 402, 1323, 1444
Acc65I GGTACC 2 cut(s) 785, 1812
AccB1I GGYRCC 3 cut(s) 707, 785, 1812
AccII CGCG 1 cut(s) 662
AccIII TCCGGA 2 cut(s) 1093, 1508
AclWI GGATC 6 cut(s) 539, 552, 873, 891, 1137, 1372
AcoI YGGCCR 1 cut(s) 194
AcsI RAATTY 4 cut(s) 115, 814, 979, 1566
AcyI GRCGYC 3 cut(s) 77, 89, 290
AfaI GTAC 5 cut(s) 17, 221, 754, 787, 1814
AfiI CCNNNNNNNGG 8 cut(s) 315, 402, 615, 1321, 1449, 1508, 1548, 1757
AgsI TTSAA 8 cut(s) 7, 151, 496, 945, 1242, 1433, 1737, 1783
AhlI ACTAGT 1 cut(s) 1039
AleI CACNNNNGTG 1 cut(s) 311
AluBI AGCT 7 cut(s) 256, 359, 642, 700, 857, 1172, 1465
AluI AGCT 7 cut(s) 256, 359, 642, 700, 857, 1172, 1465
Alw21I GWGCWC 2 cut(s) 644, 1072
Alw26I GTCTC 1 cut(s) 1570
AlwI GGATC 6 cut(s) 539, 552, 873, 891, 1137, 1372
Aor13HI TCCGGA 2 cut(s) 1093, 1508
AoxI GGCC 3 cut(s) 163, 194, 606
ApeKI GCWGC 5 cut(s) 253, 273, 673, 744, 797
ApoI RAATTY 4 cut(s) 115, 814, 979, 1566
Asp718I GGTACC 2 cut(s) 785, 1812
AspLEI GCGC 3 cut(s) 253, 1730, 1772
AspS9I GGNCC 3 cut(s) 441, 1312, 1636
AsuC2I CCSGG 2 cut(s) 310, 1581
AsuHPI GGTGA 6 cut(s) 253, 851, 1190, 1221, 1531, 1595
AsuII TTCGAA 1 cut(s) 575
AvaII GGWCC 3 cut(s) 441, 1312, 1636
BaeGI GKGCMC 2 cut(s) 712, 1188
BaeI ACNNNNGTAYC 2 cut(s) 777, 810
BamHI GGATCC 1 cut(s) 544
BanI GGYRCC 3 cut(s) 707, 785, 1812
BanII GRGCYC 3 cut(s) 44, 644, 1503
BarI GAAGNNNNNNTAC 2 cut(s) 1326, 1358
BauI CACGAG 1 cut(s) 1084
BbsI GAAGAC 2 cut(s) 87, 194
Bbv12I GWGCWC 2 cut(s) 644, 1072
BbvI GCAGC 5 cut(s) 265, 285, 685, 756, 784
BccI CCATC 6 cut(s) 720, 803, 918, 965, 995, 1091
BceAI ACGGC 2 cut(s) 34, 87
BciVI GTATCC 1 cut(s) 499
BcnI CCSGG 2 cut(s) 310, 1581
BcoDI GTCTC 1 cut(s) 1570
BcuI ACTAGT 1 cut(s) 1039
BfaI CTAG 5 cut(s) 270, 741, 1040, 1619, 1626
BfoI RGCGCY 2 cut(s) 1731, 1773
BfuAI ACCTGC 3 cut(s) 402, 1323, 1444
BfuI GTATCC 1 cut(s) 499
BisI GCNGC 9 cut(s) 197, 254, 274, 324, 327, 674, 745, 798, 1340
BlsI GCNGC 9 cut(s) 198, 255, 275, 325, 328, 675, 746, 799, 1341
Bme1390I CCNGG 2 cut(s) 310, 1581
Bme18I GGWCC 3 cut(s) 441, 1312, 1636
BmgT120I GGNCC 3 cut(s) 441, 1312, 1636
BmiI GGNNCC 8 cut(s) 442, 443, 546, 709, 771, 787, 1313, 1814
BmrFI CCNGG 2 cut(s) 310, 1581
BmrI ACTGGG 1 cut(s) 575
BmsI GCATC 4 cut(s) 36, 65, 1519, 1553
BmuI ACTGGG 1 cut(s) 575
BpiI GAAGAC 2 cut(s) 87, 194
BpmI CTGGAG 1 cut(s) 666
Bpu10I CCTNAGC 1 cut(s) 38
Bpu14I TTCGAA 1 cut(s) 575
BpuEI CTTGAG 1 cut(s) 242
BpuMI CCSGG 2 cut(s) 310, 1581
BsaBI GATNNNNATC 1 cut(s) 543
BsaHI GRCGYC 3 cut(s) 77, 89, 290
BsaI GGTCTC 1 cut(s) 1570
BsaJI CCNNGG 4 cut(s) 309, 603, 609, 937
BsaWI WCCGGW 2 cut(s) 1093, 1508
Bsc4I CCNNNNNNNGG 8 cut(s) 315, 402, 615, 1321, 1449, 1508, 1548, 1757
Bse118I RCCGGY 1 cut(s) 747
Bse1I ACTGG 6 cut(s) 581, 756, 900, 1390, 1479, 1674
Bse3DI GCAATG 1 cut(s) 406
Bse8I GATNNNNATC 1 cut(s) 543
BseAI TCCGGA 2 cut(s) 1093, 1508
BseDI CCNNGG 4 cut(s) 309, 603, 609, 937
BseGI GGATG 5 cut(s) 1102, 1330, 1394, 1534, 1578
BseJI GATNNNNATC 1 cut(s) 543
BseLI CCNNNNNNNGG 8 cut(s) 315, 402, 615, 1321, 1449, 1508, 1548, 1757
BseMI GCAATG 1 cut(s) 406
BseMII CTCAG 4 cut(s) 29, 251, 978, 1488
BseNI ACTGG 6 cut(s) 581, 756, 900, 1390, 1479, 1674
BseRI GAGGAG 2 cut(s) 197, 1634
BseSI GKGCMC 2 cut(s) 712, 1188
BseX3I CGGCCG 1 cut(s) 194
BseXI GCAGC 5 cut(s) 265, 285, 685, 756, 784
BseYI CCCAGC 2 cut(s) 853, 1442
Bsh1236I CGCG 1 cut(s) 662
Bsh1285I CGRYCG 2 cut(s) 197, 320
BshFI GGCC 3 cut(s) 165, 196, 608
BshNI GGYRCC 3 cut(s) 707, 785, 1812
BsiEI CGRYCG 2 cut(s) 197, 320
BsiHKAI GWGCWC 2 cut(s) 644, 1072
BsiSI CCGG 5 cut(s) 309, 748, 1094, 1509, 1581
BsiWI CGTACG 1 cut(s) 219
BslFI GGGAC 5 cut(s) 454, 1325, 1418, 1465, 1466
BslI CCNNNNNNNGG 8 cut(s) 315, 402, 615, 1321, 1449, 1508, 1548, 1757
BsmAI GTCTC 1 cut(s) 1570
BsmFI GGGAC 5 cut(s) 454, 1325, 1418, 1465, 1466
BsnI GGCC 3 cut(s) 165, 196, 608
Bso31I GGTCTC 1 cut(s) 1570
Bsp119I TTCGAA 1 cut(s) 575
Bsp1286I GDGCHC 6 cut(s) 44, 644, 712, 1072, 1188, 1503
Bsp13I TCCGGA 2 cut(s) 1093, 1508
Bsp143I GATC 6 cut(s) 523, 544, 865, 896, 1129, 1364
BspANI GGCC 3 cut(s) 165, 196, 608
BspCNI CTCAG 4 cut(s) 30, 250, 977, 1489
BspEI TCCGGA 2 cut(s) 1093, 1508
BspFNI CGCG 1 cut(s) 662
BspLI GGNNCC 8 cut(s) 442, 443, 546, 709, 771, 787, 1313, 1814
BspMI ACCTGC 3 cut(s) 402, 1323, 1444
BspPI GGATC 6 cut(s) 539, 552, 873, 891, 1137, 1372
BspQI GCTCTTC 1 cut(s) 1077
BspT104I TTCGAA 1 cut(s) 575
BspT107I GGYRCC 3 cut(s) 707, 785, 1812
BspTNI GGTCTC 1 cut(s) 1570
BsrDI GCAATG 1 cut(s) 406
BsrFI RCCGGY 1 cut(s) 747
BsrI ACTGG 6 cut(s) 581, 756, 900, 1390, 1479, 1674
BssAI RCCGGY 1 cut(s) 747
BssECI CCNNGG 4 cut(s) 309, 603, 609, 937
BssMI GATC 6 cut(s) 523, 544, 865, 896, 1129, 1364
BssNI GRCGYC 3 cut(s) 77, 89, 290
BssSI CACGAG 1 cut(s) 1084
BssT1I CCWWGG 2 cut(s) 609, 937
Bst2BI CACGAG 1 cut(s) 1084
Bst4CI ACNGT 5 cut(s) 247, 511, 991, 1141, 1271
Bst6I CTCTTC 3 cut(s) 1077, 1119, 1434
BstACI GRCGYC 3 cut(s) 77, 89, 290
BstAPI GCANNNNNTGC 2 cut(s) 625, 679
BstBI TTCGAA 1 cut(s) 575
BstC8I GCNNGC 3 cut(s) 278, 1448, 1682
BstDEI CTNAG 4 cut(s) 38, 237, 964, 1497
BstEII GGTNACC 1 cut(s) 1227
BstF5I GGATG 5 cut(s) 1102, 1330, 1394, 1534, 1578
BstFNI CGCG 1 cut(s) 662
BstH2I RGCGCY 2 cut(s) 1731, 1773
BstHHI GCGC 3 cut(s) 253, 1730, 1772
BstKTI GATC 6 cut(s) 526, 547, 868, 899, 1132, 1367
BstMAI GTCTC 1 cut(s) 1570
BstMBI GATC 6 cut(s) 523, 544, 865, 896, 1129, 1364
BstMCI CGRYCG 2 cut(s) 197, 320
BstMWI GCNNNNNNNGC 6 cut(s) 396, 625, 679, 803, 1386, 1767
BstNSI RCATGY 2 cut(s) 1450, 1562
BstPI GGTNACC 1 cut(s) 1227
BstSCI CCNGG 2 cut(s) 308, 1579
BstSLI GKGCMC 2 cut(s) 712, 1188
BstUI CGCG 1 cut(s) 662
BstV1I GCAGC 5 cut(s) 265, 285, 685, 756, 784
BstV2I GAAGAC 2 cut(s) 87, 194
BstX2I RGATCY 4 cut(s) 544, 896, 1129, 1364
BstYI RGATCY 4 cut(s) 544, 896, 1129, 1364
BstZI CGGCCG 1 cut(s) 194
BsuI GTATCC 1 cut(s) 499
BsuRI GGCC 3 cut(s) 165, 196, 608
BtsCI GGATG 5 cut(s) 1102, 1330, 1394, 1534, 1578
BtsI GCAGTG 1 cut(s) 1483
BtsIMutI CAGTG 8 cut(s) 526, 588, 763, 1207, 1472, 1483, 1689, 1751
BveI ACCTGC 3 cut(s) 402, 1323, 1444
Cac8I GCNNGC 3 cut(s) 278, 1448, 1682
CfoI GCGC 3 cut(s) 253, 1730, 1772
Cfr10I RCCGGY 1 cut(s) 747
Cfr13I GGNCC 3 cut(s) 441, 1312, 1636
CseI GACGC 3 cut(s) 85, 298, 651
Csp6I GTAC 5 cut(s) 16, 220, 753, 786, 1813
CviAII CATG 8 cut(s) 590, 620, 803, 890, 1150, 1194, 1447, 1559
CviQI GTAC 5 cut(s) 16, 220, 753, 786, 1813
DdeI CTNAG 4 cut(s) 38, 237, 964, 1497
DpnI GATC 6 cut(s) 525, 546, 867, 898, 1131, 1366
DpnII GATC 6 cut(s) 523, 544, 865, 896, 1129, 1364
EaeI YGGCCR 1 cut(s) 194
EagI CGGCCG 1 cut(s) 194
Eam1104I CTCTTC 3 cut(s) 1077, 1119, 1434
EarI CTCTTC 3 cut(s) 1077, 1119, 1434
Ecl136II GAGCTC 1 cut(s) 642
EclXI CGGCCG 1 cut(s) 194
Eco130I CCWWGG 2 cut(s) 609, 937
Eco24I GRGCYC 3 cut(s) 44, 644, 1503
Eco31I GGTCTC 1 cut(s) 1570
Eco47I GGWCC 3 cut(s) 441, 1312, 1636
Eco52I CGGCCG 1 cut(s) 194
Eco53kI GAGCTC 1 cut(s) 642
Eco91I GGTNACC 1 cut(s) 1227
EcoICRI GAGCTC 1 cut(s) 642
EcoO109I RGGNCCY 2 cut(s) 441, 1312
EcoO65I GGTNACC 1 cut(s) 1227
EcoT14I CCWWGG 2 cut(s) 609, 937
EcoT38I GRGCYC 3 cut(s) 44, 644, 1503
ErhI CCWWGG 2 cut(s) 609, 937
FaeI CATG 8 cut(s) 593, 623, 806, 893, 1153, 1197, 1450, 1562
FalI AAGNNNNNCTT 2 cut(s) 1116, 1148
FaqI GGGAC 5 cut(s) 454, 1325, 1418, 1465, 1466
FatI CATG 8 cut(s) 589, 619, 802, 889, 1149, 1193, 1446, 1558
FauI CCCGC 3 cut(s) 341, 422, 1443
Fnu4HI GCNGC 9 cut(s) 197, 254, 274, 324, 327, 674, 745, 798, 1340
FokI GGATG 5 cut(s) 1109, 1337, 1381, 1541, 1585
FriOI GRGCYC 3 cut(s) 44, 644, 1503
Fsp4HI GCNGC 9 cut(s) 197, 254, 274, 324, 327, 674, 745, 798, 1340
FspBI CTAG 5 cut(s) 270, 741, 1040, 1619, 1626
GlaI GCGC 3 cut(s) 252, 1729, 1771
GluI GCNGC 9 cut(s) 197, 254, 274, 324, 327, 674, 745, 798, 1340
GsaI CCCAGC 2 cut(s) 857, 1446
GsuI CTGGAG 1 cut(s) 666
HaeII RGCGCY 2 cut(s) 1731, 1773
HaeIII GGCC 3 cut(s) 165, 196, 608
HapII CCGG 5 cut(s) 309, 748, 1094, 1509, 1581
HgaI GACGC 3 cut(s) 85, 298, 651
HhaI GCGC 3 cut(s) 253, 1730, 1772
Hin1I GRCGYC 3 cut(s) 77, 89, 290
Hin1II CATG 8 cut(s) 593, 623, 806, 893, 1153, 1197, 1450, 1562
Hin6I GCGC 3 cut(s) 251, 1728, 1770
HinP1I GCGC 3 cut(s) 251, 1728, 1770
HincII GTYRAC 2 cut(s) 66, 457
HindII GTYRAC 2 cut(s) 66, 457
HindIII AAGCTT 1 cut(s) 698
HinfI GANTC 7 cut(s) 190, 463, 599, 686, 842, 1090, 1217
HpaII CCGG 5 cut(s) 309, 748, 1094, 1509, 1581
HphI GGTGA 6 cut(s) 253, 851, 1190, 1221, 1531, 1595
Hpy166II GTNNAC 6 cut(s) 66, 457, 753, 961, 1267, 1414
Hpy188I TCNGA 5 cut(s) 135, 184, 604, 1498, 1632
Hpy188III TCNNGA 6 cut(s) 645, 690, 921, 1094, 1289, 1509
Hpy8I GTNNAC 6 cut(s) 66, 457, 753, 961, 1267, 1414
Hpy99I CGWCG 6 cut(s) 55, 73, 79, 91, 94, 995
HpyAV CCTTC 4 cut(s) 155, 722, 1175, 1627
HpyCH4III ACNGT 5 cut(s) 247, 511, 991, 1141, 1271
HpyCH4IV ACGT 1 cut(s) 89
HpyCH4V TGCA 3 cut(s) 562, 682, 893
HpyF10VI GCNNNNNNNGC 6 cut(s) 396, 625, 679, 803, 1386, 1767
HpyF3I CTNAG 4 cut(s) 38, 237, 964, 1497
HpySE526I ACGT 1 cut(s) 89
Hsp92I GRCGYC 3 cut(s) 77, 89, 290
Hsp92II CATG 8 cut(s) 593, 623, 806, 893, 1153, 1197, 1450, 1562
HspAI GCGC 3 cut(s) 251, 1728, 1770
KflI GGGWCCC 1 cut(s) 441
Kpn2I TCCGGA 2 cut(s) 1093, 1508
KpnI GGTACC 2 cut(s) 789, 1816
Kzo9I GATC 6 cut(s) 523, 544, 865, 896, 1129, 1364
LguI GCTCTTC 1 cut(s) 1077
LmnI GCTCC 4 cut(s) 1146, 1308, 1444, 1741
Lsp1109I GCAGC 5 cut(s) 265, 285, 685, 756, 784
LweI GCATC 4 cut(s) 36, 65, 1519, 1553
MaeI CTAG 5 cut(s) 270, 741, 1040, 1619, 1626
MaeII ACGT 1 cut(s) 89
MaeIII GTNAC 5 cut(s) 241, 736, 1135, 1227, 1658
MalI GATC 6 cut(s) 525, 546, 867, 898, 1131, 1366
MboI GATC 6 cut(s) 523, 544, 865, 896, 1129, 1364
MfeI CAATTG 1 cut(s) 677
MflI RGATCY 4 cut(s) 544, 896, 1129, 1364
MhlI GDGCHC 6 cut(s) 44, 644, 712, 1072, 1188, 1503
MlyI GAGTC 2 cut(s) 184, 1099
MmeI TCCRAC 1 cut(s) 1394
MroI TCCGGA 2 cut(s) 1093, 1508
MseI TTAA 2 cut(s) 552, 1776
MslI CAYNNNNRTG 2 cut(s) 311, 807
MspI CCGG 5 cut(s) 309, 748, 1094, 1509, 1581
MspR9I CCNGG 2 cut(s) 310, 1581
MunI CAATTG 1 cut(s) 677
MvnI CGCG 1 cut(s) 662
MwoI GCNNNNNNNGC 6 cut(s) 396, 625, 679, 803, 1386, 1767
NciI CCSGG 2 cut(s) 310, 1581
NdeII GATC 6 cut(s) 523, 544, 865, 896, 1129, 1364
NlaIII CATG 8 cut(s) 593, 623, 806, 893, 1153, 1197, 1450, 1562
NlaIV GGNNCC 8 cut(s) 442, 443, 546, 709, 771, 787, 1313, 1814
NmeAIII GCCGAG 4 cut(s) 172, 318, 447, 1704
NmuCI GTSAC 3 cut(s) 241, 736, 1227
NspI RCATGY 2 cut(s) 1450, 1562
NspV TTCGAA 1 cut(s) 575
OliI CACNNNNGTG 1 cut(s) 311
PaeI GCATGC 1 cut(s) 1450
PciSI GCTCTTC 1 cut(s) 1077
PcsI WCGNNNNNNNCGW 4 cut(s) 59, 83, 290, 326
PfeI GAWTC 5 cut(s) 463, 599, 686, 842, 1217
Pfl23II CGTACG 1 cut(s) 219
PflFI GACNNNGTC 1 cut(s) 91
PkrI GCNGC 9 cut(s) 198, 255, 275, 325, 328, 675, 746, 799, 1341
PleI GAGTC 2 cut(s) 184, 1098
PpsI GAGTC 2 cut(s) 184, 1098
PpuMI RGGWCCY 2 cut(s) 441, 1312
PsiI TTATAA 1 cut(s) 720
Psp124BI GAGCTC 1 cut(s) 644
Psp5II RGGWCCY 2 cut(s) 441, 1312
PspEI GGTNACC 1 cut(s) 1227
PspFI CCCAGC 2 cut(s) 853, 1442
PspLI CGTACG 1 cut(s) 219
PspN4I GGNNCC 8 cut(s) 442, 443, 546, 709, 771, 787, 1313, 1814
PspPI GGNCC 3 cut(s) 441, 1312, 1636
PspPPI RGGWCCY 2 cut(s) 441, 1312
PsuI RGATCY 4 cut(s) 544, 896, 1129, 1364
PsyI GACNNNGTC 1 cut(s) 91
RsaI GTAC 5 cut(s) 17, 221, 754, 787, 1814
RsaNI GTAC 5 cut(s) 16, 220, 753, 786, 1813
RseI CAYNNNNRTG 2 cut(s) 311, 807
SacI GAGCTC 1 cut(s) 644
SapI GCTCTTC 1 cut(s) 1077
SaqAI TTAA 2 cut(s) 552, 1776
SatI GCNGC 9 cut(s) 197, 254, 274, 324, 327, 674, 745, 798, 1340
Sau3AI GATC 6 cut(s) 523, 544, 865, 896, 1129, 1364
Sau96I GGNCC 3 cut(s) 441, 1312, 1636
SchI GAGTC 2 cut(s) 184, 1099
ScrFI CCNGG 2 cut(s) 310, 1581
SduI GDGCHC 6 cut(s) 44, 644, 712, 1072, 1188, 1503
SfaNI GCATC 4 cut(s) 36, 65, 1519, 1553
SfuI TTCGAA 1 cut(s) 575
SinI GGWCC 3 cut(s) 441, 1312, 1636
SmiMI CAYNNNNRTG 2 cut(s) 311, 807
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
SpeI ACTAGT 1 cut(s) 1039
SphI GCATGC 1 cut(s) 1450
SspI AATATT 1 cut(s) 1261
SspMI CTAG 5 cut(s) 270, 741, 1040, 1619, 1626
SstI GAGCTC 1 cut(s) 644
StyD4I CCNGG 2 cut(s) 308, 1579
StyI CCWWGG 2 cut(s) 609, 937
TaaI ACNGT 5 cut(s) 247, 511, 991, 1141, 1271
TaiI ACGT 1 cut(s) 92
TaqI TCGA 5 cut(s) 434, 461, 575, 1793, 1804
TauI GCSGC 4 cut(s) 199, 326, 329, 1342
TfiI GAWTC 5 cut(s) 463, 599, 686, 842, 1217
Tru1I TTAA 2 cut(s) 552, 1776
Tru9I TTAA 2 cut(s) 552, 1776
TscAI CASTG 8 cut(s) 526, 588, 763, 1207, 1479, 1490, 1696, 1758
TseFI GTSAC 3 cut(s) 241, 736, 1227
TseI GCWGC 5 cut(s) 253, 273, 673, 744, 797
Tsp45I GTSAC 3 cut(s) 241, 736, 1227
TspDTI ATGAA 2 cut(s) 1209, 1481
TspGWI ACGGA 2 cut(s) 1265, 1809
TspRI CASTG 8 cut(s) 526, 588, 763, 1207, 1479, 1490, 1696, 1758
Tth111I GACNNNGTC 1 cut(s) 91
VpaK11BI GGWCC 3 cut(s) 441, 1312, 1636
XapI RAATTY 4 cut(s) 115, 814, 979, 1566
XceI RCATGY 2 cut(s) 1450, 1562
XcmI CCANNNNNNNNNTGG 1 cut(s) 921
XspI CTAG 5 cut(s) 270, 741, 1040, 1619, 1626
ZraI GACGTC 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.