Rh5CG425600

WD repeat-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0007846
Physical Location & Seq
Reverse (-)
58833229 .. 58838634
5406 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG425600.1

Sequence Viewer

Length: 1380 bp
ATGTACATGAACAACGGTTACAGCAGCGCCAGCACCAAAAACGGCGTCAATCTAACGTCGTCGTCGGCGGCGGCGGCGACGACATCGGGCGCAACGACGTCGTCGTCCGGTGCGCAAACCCAGTCCCTGATAAAGGCGTATTTCAAGACCCCGGAGGGGCGGTACAAGCTGCACTCGGAGAAGACGCGGCCGGCGAATCTGCTGCCCTATTCCTACGCCAAGACAATCTCGCAGGTCACGCTAGCCCAGCTGAAGGAGAGGCCCAACCAGGCGGCGGCGGGGCCGGTCCCCGGGAACATCGCCAGCGGGAGTGTGCGCCACGCGGCGGCGAGGCTGTTGGGGGTTGGGGCTGGAAATGGGAGCAAGGCGCTTAGTTATTTGGGCGGTGGGAGTAGTAAGACGAGTAATGGAAGTAGTAGGAGTGCTCATTTTGGAGGACTAGGGTCCAATGGGTATGGGGCCCATTCGTCGCCGACTTCGAGTCACGGCGGTAAAGGGTCGTTCTTGGTGTTCAATGTTGGAGATACTATCTATATTTGCGATCTCAATTCGCCAGATAAGGATCCTATTAAAGCGTTGCATTTTAGTAATTCGAATCCGGTGTGTCACGCGTTTGATTCGGAAGCCAAGGATGGGCATGACTTGCTTATTGGATTGAGCTCTGGAGATATTTATTCAGCCTCTTTGAGACAACAATTGCAAGATTCTGGGAAGAAGCTTGTTGGTGCCCTGCATTATAACAAAGAGTGTTCTGTTAATACCAGTCGCTGTACCAGTATTGCATGGGTTCCCAAAGGCAGTGGTACTTTTATTGCTGCTCATGCTGATGGAAACTTGTACGAGTATGAGAAGGGCAAGGATAGTACCGGTGATACTTCTTTCCCAGTTATAAAGGATCCTGCTCAGTTTTCAGTGTATCATGCAAGATCCAGTAAGAGTAATCCAATTTCCAGATGGCATATTTGTGAAGGTTCAATAAACAGCATTGTTTACTCTCCGGATGGGACATATTTGGCTACAGTTGGTAGAGATGGGTACTTGCGAGTATTTGACTACTCAAAAGAACAACTGATAGGTGGTGGCAAGAGTTATTATGGTTCTCTTCTATGTTGTACTTGGAGTCCGGATGGAAAATATGTTTTGACTGGAGGGGAAGATGATCTTGTTACGGTATGGAGCATGGAAGATAGGAAGGTTGTAGCTTGGGGTGAAGGACATAACTCATGGGTTAGTGGTGTGGCTTTTGACTCGCATTGGTCATCACCAACTTCGGATGGCACAGAAGAAAATCTTGTTTATCGCTTTGGCTCTGTTGGTCAGGTATTTGCTTTTCCCCTATATCTTCTCTCTCAAAAGAAAAAGACCAATTACATCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

459

Amino Acids

48.76

Weight (kDa)

8.72

Isoelectric Point (pI)

36.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WDR55 PF24796 302 - 395 7.3e-07 WDR55
Beta-prop_WDR3_1st PF25173 315 - 415 6.6e-09 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 316 - 415 7.2e-10 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 321 - 416 2.5e-12 THOC3 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 322 - 426 1e-06 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 324 - 419 8.5e-14 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 331 - 417 3.8e-08 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 738, 890
AasI GACNNNNNNGTC 1 cut(s) 103
AatII GACGTC 1 cut(s) 101
Acc16I TGCGCA 1 cut(s) 114
Acc36I ACCTGC 1 cut(s) 223
AccB1I GGYRCC 1 cut(s) 725
AccII CGCG 3 cut(s) 187, 323, 611
AccIII TCCGGA 2 cut(s) 997, 1123
AclWI GGATC 5 cut(s) 557, 570, 890, 903, 921
AcoI YGGCCR 1 cut(s) 188
AcuI CTGAAG 1 cut(s) 272
AcyI GRCGYC 2 cut(s) 45, 98
AfaI GTAC 8 cut(s) 5, 164, 772, 805, 839, 865, 1037, 1114
AfiI CCNNNNNNNGG 7 cut(s) 133, 156, 253, 274, 290, 325, 633
AflIII ACRYGT 1 cut(s) 609
AgeI ACCGGT 1 cut(s) 866
AgsI TTSAA 3 cut(s) 145, 514, 975
AhdI GACNNNNNGTC 1 cut(s) 480
AjnI CCWGG 1 cut(s) 267
AluBI AGCT 5 cut(s) 169, 250, 660, 718, 1202
AluI AGCT 5 cut(s) 169, 250, 660, 718, 1202
Alw21I GWGCWC 2 cut(s) 427, 662
Alw26I GTCTC 1 cut(s) 682
AlwI GGATC 5 cut(s) 557, 570, 890, 903, 921
AlwNI CAGNNNCTG 2 cut(s) 127, 768
Ama87I CYCGRG 1 cut(s) 290
Aor13HI TCCGGA 2 cut(s) 997, 1123
AoxI GGCC 4 cut(s) 188, 260, 281, 459
ApaI GGGCCC 1 cut(s) 463
ApeKI GCWGC 4 cut(s) 24, 169, 202, 815
ArsI GACNNNNNNTTYG 2 cut(s) 30, 62
AsiGI ACCGGT 1 cut(s) 866
AspLEI GCGC 5 cut(s) 29, 92, 115, 318, 370
AspS9I GGNCC 6 cut(s) 261, 281, 286, 444, 459, 460
AsuC2I CCSGG 3 cut(s) 152, 291, 292
AsuHPI GGTGA 3 cut(s) 881, 1220, 1254
AsuII TTCGAA 1 cut(s) 593
AsuNHI GCTAGC 1 cut(s) 241
AvaI CYCGRG 1 cut(s) 290
AvaII GGWCC 2 cut(s) 286, 444
BaeGI GKGCMC 2 cut(s) 463, 730
BamHI GGATCC 2 cut(s) 562, 895
BanI GGYRCC 1 cut(s) 725
BanII GRGCYC 2 cut(s) 463, 662
BbsI GAAGAC 1 cut(s) 188
Bbv12I GWGCWC 2 cut(s) 427, 662
BbvI GCAGC 4 cut(s) 36, 156, 189, 802
BccI CCATC 7 cut(s) 626, 821, 948, 995, 1025, 1121, 1268
BceAI ACGGC 2 cut(s) 58, 502
BcgI CGANNNNNNTGC 4 cut(s) 81, 115, 184, 218
BciT130I CCWGG 1 cut(s) 269
BcnI CCSGG 3 cut(s) 152, 291, 292
BcoDI GTCTC 1 cut(s) 682
BfaI CTAG 2 cut(s) 242, 440
BfmI CTRYAG 1 cut(s) 1017
BfoI RGCGCY 2 cut(s) 30, 371
BfuAI ACCTGC 1 cut(s) 223
Bme1390I CCNGG 4 cut(s) 152, 269, 291, 292
Bme18I GGWCC 2 cut(s) 286, 444
BmeRI GACNNNNNGTC 1 cut(s) 480
BmeT110I CYCGRG 1 cut(s) 290
BmgT120I GGNCC 6 cut(s) 261, 281, 286, 444, 459, 460
BmiI GGNNCC 9 cut(s) 282, 288, 445, 460, 461, 564, 727, 789, 897
BmrFI CCNGG 4 cut(s) 152, 269, 291, 292
BmrI ACTGGG 2 cut(s) 115, 878
BmtI GCTAGC 1 cut(s) 245
BmuI ACTGGG 2 cut(s) 115, 878
BoxI GACNNNNGTC 1 cut(s) 442
BpiI GAAGAC 1 cut(s) 188
BpmI CTGGAG 2 cut(s) 684, 1167
Bpu14I TTCGAA 1 cut(s) 593
BpuMI CCSGG 3 cut(s) 152, 291, 292
BsaBI GATNNNNATC 1 cut(s) 561
BsaHI GRCGYC 2 cut(s) 45, 98
BsaJI CCNNGG 4 cut(s) 150, 289, 290, 627
BsaWI WCCGGW 5 cut(s) 107, 598, 866, 997, 1123
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bsc4I CCNNNNNNNGG 7 cut(s) 133, 156, 253, 274, 290, 325, 633
Bse118I RCCGGY 3 cut(s) 190, 283, 866
Bse1I ACTGG 6 cut(s) 121, 762, 774, 884, 930, 1150
Bse8I GATNNNNATC 1 cut(s) 561
BseAI TCCGGA 2 cut(s) 997, 1123
BseBI CCWGG 1 cut(s) 269
BseDI CCNNGG 4 cut(s) 150, 289, 290, 627
BseGI GGATG 5 cut(s) 637, 1006, 1132, 1279, 1371
BseJI GATNNNNATC 1 cut(s) 561
BseLI CCNNNNNNNGG 7 cut(s) 133, 156, 253, 274, 290, 325, 633
BseMII CTCAG 1 cut(s) 917
BseNI ACTGG 6 cut(s) 121, 762, 774, 884, 930, 1150
BseSI GKGCMC 2 cut(s) 463, 730
BseX3I CGGCCG 1 cut(s) 188
BseXI GCAGC 4 cut(s) 36, 156, 189, 802
BseYI CCCAGC 1 cut(s) 246
BsgI GTGCAG 1 cut(s) 155
Bsh1236I CGCG 3 cut(s) 187, 323, 611
Bsh1285I CGRYCG 1 cut(s) 191
BshFI GGCC 4 cut(s) 190, 262, 283, 461
BshNI GGYRCC 1 cut(s) 725
BshTI ACCGGT 1 cut(s) 866
BsiEI CGRYCG 1 cut(s) 191
BsiHKAI GWGCWC 2 cut(s) 427, 662
BsiHKCI CYCGRG 1 cut(s) 290
BsiSI CCGG 9 cut(s) 108, 152, 191, 284, 291, 599, 867, 998, 1124
BslFI GGGAC 3 cut(s) 109, 272, 1018
BslI CCNNNNNNNGG 7 cut(s) 133, 156, 253, 274, 290, 325, 633
BsmAI GTCTC 1 cut(s) 682
BsmFI GGGAC 3 cut(s) 109, 272, 1018
BsnI GGCC 4 cut(s) 190, 262, 283, 461
BsoBI CYCGRG 1 cut(s) 290
Bsp119I TTCGAA 1 cut(s) 593
Bsp120I GGGCCC 1 cut(s) 459
Bsp1286I GDGCHC 4 cut(s) 427, 463, 662, 730
Bsp13I TCCGGA 2 cut(s) 997, 1123
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 5 cut(s) 541, 562, 895, 926, 1159
BspANI GGCC 4 cut(s) 190, 262, 283, 461
BspCNI CTCAG 1 cut(s) 916
BspEI TCCGGA 2 cut(s) 997, 1123
BspFNI CGCG 3 cut(s) 187, 323, 611
BspLI GGNNCC 9 cut(s) 282, 288, 445, 460, 461, 564, 727, 789, 897
BspMI ACCTGC 1 cut(s) 223
BspOI GCTAGC 1 cut(s) 245
BspPI GGATC 5 cut(s) 557, 570, 890, 903, 921
BspT104I TTCGAA 1 cut(s) 593
BspT107I GGYRCC 1 cut(s) 725
BsrFI RCCGGY 3 cut(s) 190, 283, 866
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 6 cut(s) 121, 762, 774, 884, 930, 1150
BssAI RCCGGY 3 cut(s) 190, 283, 866
BssECI CCNNGG 4 cut(s) 150, 289, 290, 627
BssMI GATC 5 cut(s) 541, 562, 895, 926, 1159
BssNI GRCGYC 2 cut(s) 45, 98
BssT1I CCWWGG 1 cut(s) 627
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 3 cut(s) 17, 1021, 1171
Bst6I CTCTTC 1 cut(s) 1107
BstACI GRCGYC 2 cut(s) 45, 98
BstAPI GCANNNNNTGC 1 cut(s) 643
BstAUI TGTACA 1 cut(s) 3
BstBI TTCGAA 1 cut(s) 593
BstC8I GCNNGC 4 cut(s) 31, 192, 243, 304
BstDEI CTNAG 2 cut(s) 371, 903
BstENI CCTNNNNNAGG 1 cut(s) 131
BstF5I GGATG 5 cut(s) 637, 1006, 1132, 1279, 1371
BstFNI CGCG 3 cut(s) 187, 323, 611
BstH2I RGCGCY 2 cut(s) 30, 371
BstHHI GCGC 5 cut(s) 29, 92, 115, 318, 370
BstKTI GATC 5 cut(s) 544, 565, 898, 929, 1162
BstMAI GTCTC 1 cut(s) 682
BstMBI GATC 5 cut(s) 541, 562, 895, 926, 1159
BstMCI CGRYCG 1 cut(s) 191
BstMWI GCNNNNNNNGC 7 cut(s) 30, 74, 166, 238, 247, 643, 821
BstNI CCWGG 1 cut(s) 269
BstPAI GACNNNNGTC 1 cut(s) 442
BstSCI CCNGG 4 cut(s) 150, 267, 289, 290
BstSFI CTRYAG 1 cut(s) 1017
BstSLI GKGCMC 2 cut(s) 463, 730
BstUI CGCG 3 cut(s) 187, 323, 611
BstV1I GCAGC 4 cut(s) 36, 156, 189, 802
BstV2I GAAGAC 1 cut(s) 188
BstX2I RGATCY 3 cut(s) 562, 895, 926
BstYI RGATCY 3 cut(s) 562, 895, 926
BstZI CGGCCG 1 cut(s) 188
BsuRI GGCC 4 cut(s) 190, 262, 283, 461
BtgZI GCGATG 1 cut(s) 283
BtsCI GGATG 5 cut(s) 637, 1006, 1132, 1279, 1371
BtsI GCAGTG 1 cut(s) 805
BtsIMutI CAGTG 2 cut(s) 805, 918
BveI ACCTGC 1 cut(s) 223
Cac8I GCNNGC 4 cut(s) 31, 192, 243, 304
CaiI CAGNNNCTG 2 cut(s) 127, 768
CfoI GCGC 5 cut(s) 29, 92, 115, 318, 370
Cfr10I RCCGGY 3 cut(s) 190, 283, 866
Cfr13I GGNCC 6 cut(s) 261, 281, 286, 444, 459, 460
Cfr9I CCCGGG 1 cut(s) 290
CseI GACGC 2 cut(s) 34, 193
Csp6I GTAC 8 cut(s) 4, 163, 771, 804, 838, 864, 1036, 1113
CspAI ACCGGT 1 cut(s) 866
CspCI CAANNNNNGTGG 2 cut(s) 781, 816
CviAII CATG 8 cut(s) 7, 638, 783, 821, 920, 1180, 1224, 1377
CviQI GTAC 8 cut(s) 4, 163, 771, 804, 838, 864, 1036, 1113
DdeI CTNAG 2 cut(s) 371, 903
DpnI GATC 5 cut(s) 543, 564, 897, 928, 1161
DpnII GATC 5 cut(s) 541, 562, 895, 926, 1159
DrdI GACNNNNNNGTC 1 cut(s) 103
DriI GACNNNNNGTC 1 cut(s) 480
DseDI GACNNNNNNGTC 1 cut(s) 103
EaeI YGGCCR 1 cut(s) 188
EagI CGGCCG 1 cut(s) 188
Eam1104I CTCTTC 1 cut(s) 1107
Eam1105I GACNNNNNGTC 1 cut(s) 480
EarI CTCTTC 1 cut(s) 1107
Ecl136II GAGCTC 1 cut(s) 660
EclXI CGGCCG 1 cut(s) 188
Eco130I CCWWGG 1 cut(s) 627
Eco24I GRGCYC 2 cut(s) 463, 662
Eco47I GGWCC 2 cut(s) 286, 444
Eco52I CGGCCG 1 cut(s) 188
Eco53kI GAGCTC 1 cut(s) 660
Eco57I CTGAAG 1 cut(s) 272
Eco88I CYCGRG 1 cut(s) 290
EcoICRI GAGCTC 1 cut(s) 660
EcoNI CCTNNNNNAGG 1 cut(s) 131
EcoO109I RGGNCCY 1 cut(s) 459
EcoRII CCWGG 1 cut(s) 267
EcoT14I CCWWGG 1 cut(s) 627
EcoT38I GRGCYC 2 cut(s) 463, 662
ErhI CCWWGG 1 cut(s) 627
FaeI CATG 8 cut(s) 10, 641, 786, 824, 923, 1183, 1227, 1380
FalI AAGNNNNNCTT 4 cut(s) 1146, 1178, 1275, 1307
FaqI GGGAC 3 cut(s) 109, 272, 1018
FatI CATG 8 cut(s) 6, 637, 782, 820, 919, 1179, 1223, 1376
FauI CCCGC 2 cut(s) 271, 299
FokI GGATG 5 cut(s) 644, 1013, 1139, 1286, 1358
FriOI GRGCYC 2 cut(s) 463, 662
FspBI CTAG 2 cut(s) 242, 440
FspI TGCGCA 1 cut(s) 114
GlaI GCGC 5 cut(s) 28, 91, 114, 317, 369
GsaI CCCAGC 1 cut(s) 250
GsuI CTGGAG 2 cut(s) 684, 1167
HaeII RGCGCY 2 cut(s) 30, 371
HaeIII GGCC 4 cut(s) 190, 262, 283, 461
HapII CCGG 9 cut(s) 108, 152, 191, 284, 291, 599, 867, 998, 1124
HgaI GACGC 2 cut(s) 34, 193
HhaI GCGC 5 cut(s) 29, 92, 115, 318, 370
Hin1I GRCGYC 2 cut(s) 45, 98
Hin1II CATG 8 cut(s) 10, 641, 786, 824, 923, 1183, 1227, 1380
Hin6I GCGC 5 cut(s) 27, 90, 113, 316, 368
HinP1I GCGC 5 cut(s) 27, 90, 113, 316, 368
HindIII AAGCTT 1 cut(s) 716
HinfI GANTC 7 cut(s) 196, 481, 595, 617, 704, 1120, 1247
HpaII CCGG 9 cut(s) 108, 152, 191, 284, 291, 599, 867, 998, 1124
HphI GGTGA 3 cut(s) 881, 1220, 1254
Hpy166II GTNNAC 1 cut(s) 991
Hpy188I TCNGA 3 cut(s) 178, 622, 1273
Hpy188III TCNNGA 5 cut(s) 145, 663, 951, 998, 1124
Hpy8I GTNNAC 1 cut(s) 991
Hpy99I CGWCG 8 cut(s) 61, 64, 67, 82, 100, 103, 106, 472
HpyAV CCTTC 5 cut(s) 247, 844, 962, 1186, 1205
HpyCH4III ACNGT 3 cut(s) 17, 1021, 1171
HpyCH4IV ACGT 2 cut(s) 56, 98
HpyCH4V TGCA 6 cut(s) 172, 580, 700, 733, 782, 923
HpyF10VI GCNNNNNNNGC 7 cut(s) 30, 74, 166, 238, 247, 643, 821
HpyF3I CTNAG 2 cut(s) 371, 903
HpySE526I ACGT 2 cut(s) 56, 98
Hsp92I GRCGYC 2 cut(s) 45, 98
Hsp92II CATG 8 cut(s) 10, 641, 786, 824, 923, 1183, 1227, 1380
HspAI GCGC 5 cut(s) 27, 90, 113, 316, 368
Kpn2I TCCGGA 2 cut(s) 997, 1123
KroI GCCGGC 1 cut(s) 190
KroNI GCCGGC 1 cut(s) 192
Kzo9I GATC 5 cut(s) 541, 562, 895, 926, 1159
LmnI GCTCC 2 cut(s) 360, 1176
Lsp1109I GCAGC 4 cut(s) 36, 156, 189, 802
MaeI CTAG 2 cut(s) 242, 440
MaeII ACGT 2 cut(s) 56, 98
MaeIII GTNAC 5 cut(s) 17, 235, 482, 605, 1165
MalI GATC 5 cut(s) 543, 564, 897, 928, 1161
MboI GATC 5 cut(s) 541, 562, 895, 926, 1159
MboII GAAGA 7 cut(s) 193, 724, 1094, 1166, 1196, 1295, 1334
MfeI CAATTG 1 cut(s) 695
MflI RGATCY 3 cut(s) 562, 895, 926
MhlI GDGCHC 4 cut(s) 427, 463, 662, 730
MluCI AATT 5 cut(s) 547, 589, 695, 945, 1366
MluI ACGCGT 1 cut(s) 609
MlyI GAGTC 3 cut(s) 490, 1129, 1241
MmeI TCCRAC 1 cut(s) 499
MnlI CCTC 6 cut(s) 148, 252, 324, 428, 691, 1142
MroI TCCGGA 2 cut(s) 997, 1123
MroNI GCCGGC 1 cut(s) 190
MseI TTAA 2 cut(s) 570, 756
MslI CAYNNNNRTG 2 cut(s) 825, 963
MspA1I CMGCKG 2 cut(s) 250, 306
MspI CCGG 9 cut(s) 108, 152, 191, 284, 291, 599, 867, 998, 1124
MspR9I CCNGG 4 cut(s) 152, 269, 291, 292
MunI CAATTG 1 cut(s) 695
MvaI CCWGG 1 cut(s) 269
MvnI CGCG 3 cut(s) 187, 323, 611
MwoI GCNNNNNNNGC 7 cut(s) 30, 74, 166, 238, 247, 643, 821
NaeI GCCGGC 1 cut(s) 192
NciI CCSGG 3 cut(s) 152, 291, 292
NdeII GATC 5 cut(s) 541, 562, 895, 926, 1159
NgoMIV GCCGGC 1 cut(s) 190
NheI GCTAGC 1 cut(s) 241
NlaIII CATG 8 cut(s) 10, 641, 786, 824, 923, 1183, 1227, 1380
NlaIV GGNNCC 9 cut(s) 282, 288, 445, 460, 461, 564, 727, 789, 897
NmuCI GTSAC 3 cut(s) 235, 482, 605
NsbI TGCGCA 1 cut(s) 114
NspV TTCGAA 1 cut(s) 593
PcsI WCGNNNNNNNCGW 4 cut(s) 92, 101, 191, 476
PdiI GCCGGC 1 cut(s) 192
PfeI GAWTC 4 cut(s) 196, 595, 617, 704
PflFI GACNNNGTC 1 cut(s) 100
PinAI ACCGGT 1 cut(s) 866
PleI GAGTC 3 cut(s) 489, 1128, 1241
PpsI GAGTC 3 cut(s) 489, 1128, 1241
PshAI GACNNNNGTC 1 cut(s) 442
PsiI TTATAA 2 cut(s) 738, 890
Psp124BI GAGCTC 1 cut(s) 662
Psp6I CCWGG 1 cut(s) 267
PspFI CCCAGC 1 cut(s) 246
PspGI CCWGG 1 cut(s) 267
PspN4I GGNNCC 9 cut(s) 282, 288, 445, 460, 461, 564, 727, 789, 897
PspOMI GGGCCC 1 cut(s) 459
PspPI GGNCC 6 cut(s) 261, 281, 286, 444, 459, 460
PsrI GAACNNNNNNTAC 1 cut(s) 34
PstNI CAGNNNCTG 2 cut(s) 127, 768
PsuI RGATCY 3 cut(s) 562, 895, 926
PsyI GACNNNGTC 1 cut(s) 100
PvuII CAGCTG 1 cut(s) 250
RsaI GTAC 8 cut(s) 5, 164, 772, 805, 839, 865, 1037, 1114
RsaNI GTAC 8 cut(s) 4, 163, 771, 804, 838, 864, 1036, 1113
RseI CAYNNNNRTG 2 cut(s) 825, 963
SacI GAGCTC 1 cut(s) 662
SaqAI TTAA 2 cut(s) 570, 756
Sau3AI GATC 5 cut(s) 541, 562, 895, 926, 1159
Sau96I GGNCC 6 cut(s) 261, 281, 286, 444, 459, 460
SchI GAGTC 3 cut(s) 490, 1129, 1241
ScrFI CCNGG 4 cut(s) 152, 269, 291, 292
SduI GDGCHC 4 cut(s) 427, 463, 662, 730
SfcI CTRYAG 1 cut(s) 1017
SfuI TTCGAA 1 cut(s) 593
SinI GGWCC 2 cut(s) 286, 444
SmaI CCCGGG 1 cut(s) 292
SmiMI CAYNNNNRTG 2 cut(s) 825, 963
Sse9I AATT 5 cut(s) 547, 589, 695, 945, 1366
SspMI CTAG 2 cut(s) 242, 440
SstI GAGCTC 1 cut(s) 662
StyD4I CCNGG 4 cut(s) 150, 267, 289, 290
StyI CCWWGG 1 cut(s) 627
TaaI ACNGT 3 cut(s) 17, 1021, 1171
TaiI ACGT 2 cut(s) 59, 101
TaqI TCGA 2 cut(s) 479, 593
TasI AATT 5 cut(s) 547, 589, 695, 945, 1366
TatI WGTACW 2 cut(s) 3, 1112
TauI GCSGC 8 cut(s) 71, 74, 77, 190, 275, 278, 326, 329
TfiI GAWTC 4 cut(s) 196, 595, 617, 704
Tru1I TTAA 2 cut(s) 570, 756
Tru9I TTAA 2 cut(s) 570, 756
TscAI CASTG 2 cut(s) 805, 918
TseFI GTSAC 3 cut(s) 235, 482, 605
TseI GCWGC 4 cut(s) 24, 169, 202, 815
Tsp45I GTSAC 3 cut(s) 235, 482, 605
TspDTI ATGAA 1 cut(s) 23
TspMI CCCGGG 1 cut(s) 290
TspRI CASTG 2 cut(s) 805, 918
Tth111I GACNNNGTC 1 cut(s) 100
VpaK11BI GGWCC 2 cut(s) 286, 444
XagI CCTNNNNNAGG 1 cut(s) 131
XcmI CCANNNNNNNNNTGG 1 cut(s) 951
XmaI CCCGGG 1 cut(s) 290
XspI CTAG 2 cut(s) 242, 440
ZraI GACGTC 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.