MD11G1133300.v1.1

WD repeat-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007846
Physical Location & Seq
Forward (+)
12226209 .. 12230851
4643 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1133300.v1.1.491

Sequence Viewer

Length: 1941 bp
ATGTTCAACAACGGCTACTCCAACAACAAACACGGCCTGAGCCCGATGTTGTCGGCATCATCGTCAACGACTGCGACGCCGCAAACGACGTCGTCTTCTTCGTCTGCAACGACGAATTCCCAGACACAATCGCTTATAAAGGCGTATTTCAAAACCCCAGAAGGCCGATACAAGCTTCACTCGGAGAAGACTCGGCCGCCCAACCTCCTCCCGTACTCGTACGCCAAGACAATTTCTCAGGTGACGGTAGCGCAGCTCAAGGACAAGCCTAGCAGCCAGCCTACGCTGGCGCCGAGTTTCAGCACCACCGGAGTGCGGTCGGCGGCGGCGAGGCTGTTGGGGGGAAATGGGGGCAAAGCTCTGAGCTTTGTGGGAGGGAGTGGATATAGCAGAACCAGCAATGGGAGTAGTAGGTATGCTCATTTCGGCGGGTCGAATGGGACCCATCACACGGTTAACTCGAATCTCGGTAGTAAAGGGTCGTTTTTGGTATTCAATGTTGGGGATACTGTTTATATCAGTGATCTCAATTCGCCCGATAAGGATCCTATCAAGGCTTTGCAATTTGGCAATTCCAATCCTGTGTGCCACGCGTTTGATTCCGAGGCCAAAGACGGGCATGACTTGCTTATTGGATTGAGCTCCGGAGATAGTAAGGAATCTTGTTTGCGTGTGATTGAATGGGGTTTTGGTGATGAGGAGGCTGTTTGTGACTTGGTTAATTTGTTTGTTGTTGCAGTTTATTCGGCGTCTTTGAGGCAGCAATTGCAAGATTCTGGAAAGAAGCTTGTTGGTGCCCATCATTATAACAAAGAAGGTTCTGTCACTAGCAGGCAAGATTGTTACTCAACTTTGCCTCATTTCCTTCTGCTTTGTTATCTATGTACTACAAGTTTGTTGATGAAAAAAGGTGTCAGGAATGCACATTGTCACTGCTGCCTGCTGGTGTGCATTCGGTGTGCCAGTGTTGCATGGGTTCCCAAAGGTAGCGGTGCTTTTGCTGCTGCTCATGCTGATGGAAACTTGTATAGTAAGGAAAGTGCTGGTGATTCTTCTTTCCCGGTTATAAGGGATCATACTCAATTTTTGGTGGCTCATGCAAGATCCAGTAAGAGTAATCCAATTTCCAGATGGCATATTTGCCAAGGTGCAATAAACAGCATTGTTTACTCAGAAGATGGGGCAAATTTGGCAACAGTCGGTAGAGATGGATATTTGCGAGTATTTGACTACTCAAAAGAACAGCTAGTATTCGGTGGCAAGAGTTATTATGGTGCTCTTCTGTGTTGTGCGTGGAGTCCGGATGGGAAATACATTTTGACAGGAGGAGAAGAGGATCTCGTTACGGTATGGAGCATGGAAGATAGAAAAGTAGTAGCTTGGGGTGAAGGGCACAACTCATGGGTTAGTGGTGTGGCGTTTGATTCATATTGGTCCCCACCAAGTTCAGATGGCACGGAAGAAAATATTGTTTACCGTTTTGGTTCTGTGGGTCAGGACACAAAATTGCTCCTATGGGACCTGCCCATGGATGAGATTGTAGTGCCACTTCGCCGAAGTTCACTTGGTGGATCTCCAACGTTCAGCACAGGTAGCCAGTCATCCCACTGGGACAGCGTTTACCCCATTGGCACTCTTCAACCTGCACCCAGCATGCGGGACATTCCAAAGCTCTCCCCACTAGTGTCTCACTGCGTTCATTCTGAGCCCCTATCCGGATTAGTTTTCACAGAGGAAACTATTCTCACTGCATCTTGGGAAGGGCATGTAAAAATTTGGATGAGACCCAGCGAACCTGAAAATAAGTCAAACGCAGAAGCCCTGCTAGGTGCTAGTCCAAAGGACCAACCACTCACAGGCAAAGGTTACACAGAACCATCTAAAAGCAAGCCGTTCAGTGAAAGTAAATCGAAACAGCATGGATACCCTGCTCGGTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

647

Amino Acids

69.73

Weight (kDa)

7.84

Isoelectric Point (pI)

47.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_TEP1_2nd PF25047 359 - 473 1.8e-06 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 372 - 512 4.9e-10 WDR3 first beta-propeller domain
WDR55 PF24796 372 - 454 7.7e-06 WDR55
Beta-prop_THOC3 PF25174 382 - 474 2.2e-10 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 384 - 475 2e-11 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 395 - 475 2.3e-07 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_THOC3 PF25174 429 - 475 6.9e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 137, 807, 1067
AatII GACGTC 1 cut(s) 92
Acc36I ACCTGC 2 cut(s) 1530, 1651
AccB1I GGYRCC 2 cut(s) 289, 794
AccII CGCG 1 cut(s) 593
AccIII TCCGGA 3 cut(s) 644, 1300, 1715
AciI CCGC 8 cut(s) 80, 197, 316, 323, 326, 429, 990, 1657
AclI AACGTT 1 cut(s) 1580
AclWI GGATC 6 cut(s) 539, 552, 1080, 1098, 1344, 1579
AcoI YGGCCR 1 cut(s) 194
AcsI RAATTY 3 cut(s) 115, 1186, 1773
AcyI GRCGYC 4 cut(s) 77, 89, 290, 749
AdeI CACNNNGTG 1 cut(s) 1568
AfaI GTAC 3 cut(s) 215, 221, 886
AfiI CCNNNNNNNGG 8 cut(s) 315, 402, 451, 615, 1528, 1656, 1715, 1856
AflIII ACRYGT 1 cut(s) 591
AgsI TTSAA 5 cut(s) 7, 151, 496, 680, 1640
AhlI ACTAGT 1 cut(s) 1681
AjuI GAANNNNNNNTTGG 2 cut(s) 672, 704
AleI CACNNNNGTG 1 cut(s) 311
AluBI AGCT 9 cut(s) 175, 256, 359, 366, 642, 787, 1246, 1379, 1672
AluI AGCT 9 cut(s) 175, 256, 359, 366, 642, 787, 1246, 1379, 1672
Alw21I GWGCWC 2 cut(s) 644, 1279
Alw26I GTCTC 2 cut(s) 1692, 1777
AlwI GGATC 6 cut(s) 539, 552, 1080, 1098, 1344, 1579
Aor13HI TCCGGA 3 cut(s) 644, 1300, 1715
AoxI GGCC 4 cut(s) 34, 163, 194, 606
ApeKI GCWGC 6 cut(s) 253, 273, 760, 936, 1001, 1004
ApoI RAATTY 3 cut(s) 115, 1186, 1773
Asp700I GAANNNNTTC 1 cut(s) 1740
AspLEI GCGC 2 cut(s) 253, 292
AspS9I GGNCC 4 cut(s) 441, 1434, 1519, 1843
AsuC2I CCSGG 1 cut(s) 1061
AsuHPI GGTGA 4 cut(s) 253, 704, 1058, 1397
AvaII GGWCC 4 cut(s) 441, 1434, 1519, 1843
BaeGI GKGCMC 2 cut(s) 799, 1395
BamHI GGATCC 1 cut(s) 544
BanI GGYRCC 2 cut(s) 289, 794
BanII GRGCYC 3 cut(s) 44, 644, 1710
BarI GAAGNNNNNNTAC 2 cut(s) 1533, 1565
BbsI GAAGAC 2 cut(s) 87, 194
Bbv12I GWGCWC 2 cut(s) 644, 1279
BbvI GCAGC 6 cut(s) 265, 285, 772, 923, 988, 991
BccI CCATC 9 cut(s) 453, 807, 1010, 1125, 1172, 1202, 1298, 1445, 1885
BceAI ACGGC 3 cut(s) 28, 49, 1876
BcgI CGANNNNNNTGC 2 cut(s) 726, 760
BciVI GTATCC 2 cut(s) 499, 1916
BcnI CCSGG 1 cut(s) 1061
BcoDI GTCTC 2 cut(s) 1692, 1777
BcuI ACTAGT 1 cut(s) 1681
BfaI CTAG 7 cut(s) 270, 828, 1247, 1682, 1826, 1833, 1939
BfoI RGCGCY 1 cut(s) 293
BfuAI ACCTGC 2 cut(s) 1530, 1651
BfuI GTATCC 2 cut(s) 499, 1916
Bme1390I CCNGG 1 cut(s) 1061
Bme18I GGWCC 4 cut(s) 441, 1434, 1519, 1843
BmgT120I GGNCC 4 cut(s) 441, 1434, 1519, 1843
BmiI GGNNCC 8 cut(s) 291, 442, 443, 546, 796, 978, 1436, 1520
BmrFI CCNGG 1 cut(s) 1061
BmrI ACTGGG 1 cut(s) 1618
BmsI GCATC 2 cut(s) 65, 1760
BmuI ACTGGG 1 cut(s) 1618
BpiI GAAGAC 2 cut(s) 87, 194
Bpu10I CCTNAGC 1 cut(s) 38
BpuEI CTTGAG 1 cut(s) 242
BpuMI CCSGG 1 cut(s) 1061
BsaBI GATNNNNATC 1 cut(s) 543
BsaHI GRCGYC 4 cut(s) 77, 89, 290, 749
BsaI GGTCTC 1 cut(s) 1777
BsaJI CCNNGG 3 cut(s) 603, 1144, 1527
BsaWI WCCGGW 4 cut(s) 308, 644, 1300, 1715
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bsc4I CCNNNNNNNGG 8 cut(s) 315, 402, 451, 615, 1528, 1656, 1715, 1856
Bse1I ACTGG 4 cut(s) 963, 1107, 1597, 1613
Bse3DI GCAATG 1 cut(s) 406
Bse8I GATNNNNATC 1 cut(s) 543
BseAI TCCGGA 3 cut(s) 644, 1300, 1715
BseDI CCNNGG 3 cut(s) 603, 1144, 1527
BseGI GGATG 4 cut(s) 1309, 1537, 1601, 1785
BseJI GATNNNNATC 1 cut(s) 543
BseLI CCNNNNNNNGG 8 cut(s) 315, 402, 451, 615, 1528, 1656, 1715, 1856
BseMI GCAATG 1 cut(s) 406
BseMII CTCAG 5 cut(s) 29, 251, 353, 1185, 1695
BseNI ACTGG 4 cut(s) 963, 1107, 1597, 1613
BseRI GAGGAG 3 cut(s) 197, 713, 1341
BseSI GKGCMC 2 cut(s) 799, 1395
BseX3I CGGCCG 1 cut(s) 194
BseXI GCAGC 6 cut(s) 265, 285, 772, 923, 988, 991
BseYI CCCAGC 2 cut(s) 1649, 1787
BsgI GTGCAG 1 cut(s) 1629
Bsh1236I CGCG 1 cut(s) 593
Bsh1285I CGRYCG 2 cut(s) 197, 320
BshFI GGCC 4 cut(s) 36, 165, 196, 608
BshNI GGYRCC 2 cut(s) 289, 794
BsiEI CGRYCG 2 cut(s) 197, 320
BsiHKAI GWGCWC 2 cut(s) 644, 1279
BsiSI CCGG 5 cut(s) 309, 645, 1061, 1301, 1716
BsiWI CGTACG 1 cut(s) 219
BslFI GGGAC 5 cut(s) 454, 1420, 1532, 1625, 1673
BslI CCNNNNNNNGG 8 cut(s) 315, 402, 451, 615, 1528, 1656, 1715, 1856
BsmAI GTCTC 2 cut(s) 1692, 1777
BsmFI GGGAC 5 cut(s) 454, 1420, 1532, 1625, 1673
BsmI GAATGC 2 cut(s) 925, 951
BsnI GGCC 4 cut(s) 36, 165, 196, 608
Bso31I GGTCTC 1 cut(s) 1777
Bsp1286I GDGCHC 6 cut(s) 44, 644, 799, 1279, 1395, 1710
Bsp13I TCCGGA 3 cut(s) 644, 1300, 1715
Bsp143I GATC 6 cut(s) 523, 544, 1072, 1103, 1336, 1571
Bsp19I CCATGG 1 cut(s) 1527
BspACI CCGC 8 cut(s) 80, 197, 316, 323, 326, 429, 990, 1657
BspANI GGCC 4 cut(s) 36, 165, 196, 608
BspCNI CTCAG 5 cut(s) 30, 250, 354, 1184, 1696
BspEI TCCGGA 3 cut(s) 644, 1300, 1715
BspFNI CGCG 1 cut(s) 593
BspLI GGNNCC 8 cut(s) 291, 442, 443, 546, 796, 978, 1436, 1520
BspMI ACCTGC 2 cut(s) 1530, 1651
BspPI GGATC 6 cut(s) 539, 552, 1080, 1098, 1344, 1579
BspQI GCTCTTC 1 cut(s) 1284
BspT107I GGYRCC 2 cut(s) 289, 794
BspTNI GGTCTC 1 cut(s) 1777
BsrDI GCAATG 1 cut(s) 406
BsrI ACTGG 4 cut(s) 963, 1107, 1597, 1613
BssECI CCNNGG 3 cut(s) 603, 1144, 1527
BssMI GATC 6 cut(s) 523, 544, 1072, 1103, 1336, 1571
BssNI GRCGYC 4 cut(s) 77, 89, 290, 749
BssT1I CCWWGG 2 cut(s) 1144, 1527
Bst4CI ACNGT 6 cut(s) 247, 454, 511, 1198, 1348, 1478
Bst6I CTCTTC 3 cut(s) 1284, 1326, 1641
BstACI GRCGYC 4 cut(s) 77, 89, 290, 749
BstAPI GCANNNNNTGC 2 cut(s) 625, 766
BstC8I GCNNGC 6 cut(s) 278, 288, 833, 941, 1655, 1889
BstDEI CTNAG 5 cut(s) 38, 237, 362, 1171, 1704
BstDSI CCRYGG 1 cut(s) 1527
BstF5I GGATG 4 cut(s) 1309, 1537, 1601, 1785
BstFNI CGCG 1 cut(s) 593
BstH2I RGCGCY 1 cut(s) 293
BstHHI GCGC 2 cut(s) 253, 292
BstKTI GATC 6 cut(s) 526, 547, 1075, 1106, 1339, 1574
BstMAI GTCTC 2 cut(s) 1692, 1777
BstMBI GATC 6 cut(s) 523, 544, 1072, 1103, 1336, 1571
BstMCI CGRYCG 2 cut(s) 197, 320
BstMWI GCNNNNNNNGC 8 cut(s) 396, 625, 766, 968, 1001, 1010, 1190, 1593
BstNSI RCATGY 2 cut(s) 1657, 1769
BstSCI CCNGG 1 cut(s) 1059
BstSLI GKGCMC 2 cut(s) 799, 1395
BstUI CGCG 1 cut(s) 593
BstV1I GCAGC 6 cut(s) 265, 285, 772, 923, 988, 991
BstV2I GAAGAC 2 cut(s) 87, 194
BstX2I RGATCY 4 cut(s) 544, 1103, 1336, 1571
BstYI RGATCY 4 cut(s) 544, 1103, 1336, 1571
BstZI CGGCCG 1 cut(s) 194
BsuI GTATCC 2 cut(s) 499, 1916
BsuRI GGCC 4 cut(s) 36, 165, 196, 608
BtgI CCRYGG 1 cut(s) 1527
BtsCI GGATG 4 cut(s) 1309, 1537, 1601, 1785
BtsI GCAGTG 3 cut(s) 931, 1690, 1746
BtsIMutI CAGTG 7 cut(s) 526, 931, 970, 1606, 1690, 1746, 1903
BveI ACCTGC 2 cut(s) 1530, 1651
Cac8I GCNNGC 6 cut(s) 278, 288, 833, 941, 1655, 1889
CfoI GCGC 2 cut(s) 253, 292
Cfr13I GGNCC 4 cut(s) 441, 1434, 1519, 1843
CseI GACGC 2 cut(s) 85, 738
Csp6I GTAC 3 cut(s) 214, 220, 885
CviQI GTAC 3 cut(s) 214, 220, 885
DdeI CTNAG 5 cut(s) 38, 237, 362, 1171, 1704
DinI GGCGCC 1 cut(s) 291
DpnI GATC 6 cut(s) 525, 546, 1074, 1105, 1338, 1573
DpnII GATC 6 cut(s) 523, 544, 1072, 1103, 1336, 1571
DraIII CACNNNGTG 1 cut(s) 1568
EaeI YGGCCR 1 cut(s) 194
EagI CGGCCG 1 cut(s) 194
Eam1104I CTCTTC 3 cut(s) 1284, 1326, 1641
EarI CTCTTC 3 cut(s) 1284, 1326, 1641
Ecl136II GAGCTC 1 cut(s) 642
EclXI CGGCCG 1 cut(s) 194
Eco130I CCWWGG 2 cut(s) 1144, 1527
Eco24I GRGCYC 3 cut(s) 44, 644, 1710
Eco31I GGTCTC 1 cut(s) 1777
Eco47I GGWCC 4 cut(s) 441, 1434, 1519, 1843
Eco52I CGGCCG 1 cut(s) 194
Eco53kI GAGCTC 1 cut(s) 642
EcoICRI GAGCTC 1 cut(s) 642
EcoO109I RGGNCCY 2 cut(s) 441, 1519
EcoRI GAATTC 1 cut(s) 115
EcoT14I CCWWGG 2 cut(s) 1144, 1527
EcoT38I GRGCYC 3 cut(s) 44, 644, 1710
EgeI GGCGCC 1 cut(s) 291
EheI GGCGCC 1 cut(s) 291
ErhI CCWWGG 2 cut(s) 1144, 1527
FaqI GGGAC 5 cut(s) 454, 1420, 1532, 1625, 1673
FauI CCCGC 2 cut(s) 422, 1650
FokI GGATG 4 cut(s) 1316, 1544, 1588, 1792
FriOI GRGCYC 3 cut(s) 44, 644, 1710
FspBI CTAG 7 cut(s) 270, 828, 1247, 1682, 1826, 1833, 1939
GlaI GCGC 2 cut(s) 252, 291
GsaI CCCAGC 2 cut(s) 1653, 1791
HaeII RGCGCY 1 cut(s) 293
HaeIII GGCC 4 cut(s) 36, 165, 196, 608
HapII CCGG 5 cut(s) 309, 645, 1061, 1301, 1716
HgaI GACGC 2 cut(s) 85, 738
HhaI GCGC 2 cut(s) 253, 292
Hin1I GRCGYC 4 cut(s) 77, 89, 290, 749
Hin6I GCGC 2 cut(s) 251, 290
HinP1I GCGC 2 cut(s) 251, 290
HincII GTYRAC 2 cut(s) 66, 457
HindII GTYRAC 2 cut(s) 66, 457
HindIII AAGCTT 2 cut(s) 173, 785
HinfI GANTC 8 cut(s) 190, 463, 599, 659, 773, 1049, 1297, 1424
HpaI GTTAAC 1 cut(s) 457
HpaII CCGG 5 cut(s) 309, 645, 1061, 1301, 1716
HphI GGTGA 4 cut(s) 253, 704, 1058, 1397
Hpy166II GTNNAC 6 cut(s) 66, 457, 1168, 1474, 1562, 1621
Hpy188I TCNGA 6 cut(s) 184, 363, 604, 1174, 1450, 1705
Hpy188III TCNNGA 7 cut(s) 645, 777, 916, 1128, 1301, 1496, 1716
Hpy8I GTNNAC 6 cut(s) 66, 457, 1168, 1474, 1562, 1621
Hpy99I CGWCG 4 cut(s) 79, 91, 94, 115
HpyAV CCTTC 5 cut(s) 155, 809, 875, 1382, 1754
HpyCH4III ACNGT 6 cut(s) 247, 454, 511, 1198, 1348, 1478
HpyCH4IV ACGT 2 cut(s) 89, 1580
HpyF10VI GCNNNNNNNGC 8 cut(s) 396, 625, 766, 968, 1001, 1010, 1190, 1593
HpyF3I CTNAG 5 cut(s) 38, 237, 362, 1171, 1704
HpySE526I ACGT 2 cut(s) 89, 1580
Hsp92I GRCGYC 4 cut(s) 77, 89, 290, 749
HspAI GCGC 2 cut(s) 251, 290
KasI GGCGCC 1 cut(s) 289
KflI GGGWCCC 1 cut(s) 441
Kpn2I TCCGGA 3 cut(s) 644, 1300, 1715
KspAI GTTAAC 1 cut(s) 457
Kzo9I GATC 6 cut(s) 523, 544, 1072, 1103, 1336, 1571
LguI GCTCTTC 1 cut(s) 1284
LmnI GCTCC 3 cut(s) 647, 1353, 1515
Lsp1109I GCAGC 6 cut(s) 265, 285, 772, 923, 988, 991
LweI GCATC 2 cut(s) 65, 1760
MaeI CTAG 7 cut(s) 270, 828, 1247, 1682, 1826, 1833, 1939
MaeII ACGT 2 cut(s) 89, 1580
MaeIII GTNAC 7 cut(s) 241, 710, 823, 842, 929, 1342, 1865
MalI GATC 6 cut(s) 525, 546, 1074, 1105, 1338, 1573
MboI GATC 6 cut(s) 523, 544, 1072, 1103, 1336, 1571
MfeI CAATTG 1 cut(s) 764
MflI RGATCY 4 cut(s) 544, 1103, 1336, 1571
MhlI GDGCHC 6 cut(s) 44, 644, 799, 1279, 1395, 1710
MluI ACGCGT 1 cut(s) 591
Mly113I GGCGCC 1 cut(s) 290
MlyI GAGTC 2 cut(s) 184, 1306
MmeI TCCRAC 2 cut(s) 45, 1601
MroI TCCGGA 3 cut(s) 644, 1300, 1715
MroXI GAANNNNTTC 1 cut(s) 1740
MseI TTAA 2 cut(s) 456, 720
MslI CAYNNNNRTG 2 cut(s) 311, 1014
MspI CCGG 5 cut(s) 309, 645, 1061, 1301, 1716
MspR9I CCNGG 1 cut(s) 1061
MunI CAATTG 1 cut(s) 764
Mva1269I GAATGC 2 cut(s) 925, 951
MvnI CGCG 1 cut(s) 593
MwoI GCNNNNNNNGC 8 cut(s) 396, 625, 766, 968, 1001, 1010, 1190, 1593
NarI GGCGCC 1 cut(s) 290
NciI CCSGG 1 cut(s) 1061
NcoI CCATGG 1 cut(s) 1527
NdeII GATC 6 cut(s) 523, 544, 1072, 1103, 1336, 1571
NlaIV GGNNCC 8 cut(s) 291, 442, 443, 546, 796, 978, 1436, 1520
NmeAIII GCCGAG 2 cut(s) 172, 318
NmuCI GTSAC 4 cut(s) 241, 710, 823, 929
NspI RCATGY 2 cut(s) 1657, 1769
OliI CACNNNNGTG 1 cut(s) 311
PaeI GCATGC 1 cut(s) 1657
PciSI GCTCTTC 1 cut(s) 1284
PcsI WCGNNNNNNNCGW 7 cut(s) 59, 83, 98, 107, 290, 326, 458
PctI GAATGC 2 cut(s) 925, 951
PdmI GAANNNNTTC 1 cut(s) 1740
PfeI GAWTC 6 cut(s) 463, 599, 659, 773, 1049, 1424
Pfl23II CGTACG 1 cut(s) 219
PflFI GACNNNGTC 1 cut(s) 91
PleI GAGTC 2 cut(s) 184, 1305
PluTI GGCGCC 1 cut(s) 293
PpsI GAGTC 2 cut(s) 184, 1305
PpuMI RGGWCCY 2 cut(s) 441, 1519
PsiI TTATAA 3 cut(s) 137, 807, 1067
Psp124BI GAGCTC 1 cut(s) 644
Psp1406I AACGTT 1 cut(s) 1580
Psp5II RGGWCCY 2 cut(s) 441, 1519
PspFI CCCAGC 2 cut(s) 1649, 1787
PspLI CGTACG 1 cut(s) 219
PspN4I GGNNCC 8 cut(s) 291, 442, 443, 546, 796, 978, 1436, 1520
PspPI GGNCC 4 cut(s) 441, 1434, 1519, 1843
PspPPI RGGWCCY 2 cut(s) 441, 1519
PsuI RGATCY 4 cut(s) 544, 1103, 1336, 1571
PsyI GACNNNGTC 1 cut(s) 91
RsaI GTAC 3 cut(s) 215, 221, 886
RsaNI GTAC 3 cut(s) 214, 220, 885
RseI CAYNNNNRTG 2 cut(s) 311, 1014
SacI GAGCTC 1 cut(s) 644
SapI GCTCTTC 1 cut(s) 1284
SaqAI TTAA 2 cut(s) 456, 720
Sau3AI GATC 6 cut(s) 523, 544, 1072, 1103, 1336, 1571
Sau96I GGNCC 4 cut(s) 441, 1434, 1519, 1843
SchI GAGTC 2 cut(s) 184, 1306
ScrFI CCNGG 1 cut(s) 1061
SduI GDGCHC 6 cut(s) 44, 644, 799, 1279, 1395, 1710
SfaNI GCATC 2 cut(s) 65, 1760
SfoI GGCGCC 1 cut(s) 291
SinI GGWCC 4 cut(s) 441, 1434, 1519, 1843
SmiMI CAYNNNNRTG 2 cut(s) 311, 1014
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
SpeI ACTAGT 1 cut(s) 1681
SphI GCATGC 1 cut(s) 1657
SsiI CCGC 8 cut(s) 80, 197, 316, 323, 326, 429, 990, 1657
SspDI GGCGCC 1 cut(s) 289
SspI AATATT 1 cut(s) 1468
SspMI CTAG 7 cut(s) 270, 828, 1247, 1682, 1826, 1833, 1939
SstI GAGCTC 1 cut(s) 644
StyD4I CCNGG 1 cut(s) 1059
StyI CCWWGG 2 cut(s) 1144, 1527
TaaI ACNGT 6 cut(s) 247, 454, 511, 1198, 1348, 1478
TaiI ACGT 2 cut(s) 92, 1583
TaqI TCGA 3 cut(s) 434, 461, 1910
TatI WGTACW 1 cut(s) 884
TauI GCSGC 4 cut(s) 82, 199, 326, 329
TfiI GAWTC 6 cut(s) 463, 599, 659, 773, 1049, 1424
Tru1I TTAA 2 cut(s) 456, 720
Tru9I TTAA 2 cut(s) 456, 720
TscAI CASTG 7 cut(s) 526, 938, 970, 1613, 1697, 1753, 1903
TseFI GTSAC 4 cut(s) 241, 710, 823, 929
TseI GCWGC 6 cut(s) 253, 273, 760, 936, 1001, 1004
Tsp45I GTSAC 4 cut(s) 241, 710, 823, 929
TspDTI ATGAA 3 cut(s) 917, 1416, 1688
TspGWI ACGGA 1 cut(s) 1472
TspRI CASTG 7 cut(s) 526, 938, 970, 1613, 1697, 1753, 1903
Tth111I GACNNNGTC 1 cut(s) 91
VpaK11BI GGWCC 4 cut(s) 441, 1434, 1519, 1843
XapI RAATTY 3 cut(s) 115, 1186, 1773
XceI RCATGY 2 cut(s) 1657, 1769
XcmI CCANNNNNNNNNTGG 1 cut(s) 1128
XmnI GAANNNNTTC 1 cut(s) 1740
XspI CTAG 7 cut(s) 270, 828, 1247, 1682, 1826, 1833, 1939
ZraI GACGTC 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.