Rw5G036690

WD repeat-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0007846
Physical Location & Seq
Reverse (-)
64345355 .. 64348767
3413 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G036690.1

Sequence Viewer

Length: 1767 bp
ATGTACATGAACAACGGTTACAGCAGCGCCAGCACCAAAAACGGCGTCAATCTAACGTCGTCGTCGTCGGCGGCGACGACATCGGGCGCAACGACGTCGTCGTCCGGTGCGCAAACCCAGTCCCTAATAAAGGCGTATTTCAAGACCCCGGAGGGGCGGTACAAGCTGCACTCGGAGAAGACGCGGCCGGCGAATCTGCTGCCCTATTCGTACGCCAAGACAATCTCGCAGGTCACGCTAGCCCAGCTGAAGGAGAGGCCCAACCAGGCGGCGCCGGGGCCGGTCCCCGGGAACATCGCCAGCGGGAGTGTGCGCCACGCGGCGGCGAGGCTGTTGGGGGTTGGGGCTGGAAATGGGAGCAAGGCGCTTAGTTATTTGGGCGGTGGGAGTAGTAAGACGAGTAATGGAAGTAGTAGGAGTGCTCATTTTGGAGGACTAGGGTCCAATGGGTATGGGGCCCATTCGTCGCCGACTTCGAGTCACGGCGGTAAAGGGTCGTTCTTGGTGTTCAATGTTGGAGATACTATCTATATTTGCGATCTCAATTCGCCAGATAAGGATCCTATTAAAGCGTTGCATTTTAGTAATTCGAATCCGGTGTGTCACGCGTTTGATTCGGAAGCCAAGGATGGGCATGACTTGCTTATTGGATTGAGCTCTGGAGATATTTATTCAGCCTCTTTGAGACAGCAATTGCAAGATTCTGGGAAGAAGCTTGTTGGTGCCCTGCATTATAACAAAGAGGGTTCTGTTAATACCAGTCGCTGTACCAGTATTGCATGGGTTCCCAAAGGCAATGGTACTTTTATTGCTGCTCATGCTGATGGAAACTTGTACGAGTATGAGAAGGGCAAGGATATTACCGGTGATACTTCTTTCCCAGTTATAAAGGATCCTGCTCAGTTTTCAGTGTATCATGCAAGATCCAGTAAGAGTAATCCAATTTCCAGATGGCATATTTGTGAAGGTTCACTAAACAGCATTGTTTACTCTCCGGATGGGACATATTTGGCTACAGTTGGTAGAGATGGGTACTTGCGAGTATTTGACTACTCAAAAGAACAACTGATAGGTGGCGGCAAGAGTTATTATGGTTCTCTTCTATGTTGTACTTGGAGTCCGGATGGAAAATATGTTTTGACTGGAGGGGAAGATGATCTTGTTACAGTATGGAGCATGGAAGATAGGAAGGTTATAGCTTGGGGTGAAGGACATAACTCATGGGTTAGTGGTTTGGCTTTTGACTCGCATTGGTCATCACCAACTTCGGATGGCACAGAAGAAAATCTTGTTTATCGCTTTGGCTCTGTTGGTCAGGACACAAAACTACTCCTGTGGGAACTCTCCATGGATGAGATTGTAGTGCCGCTTCGCCGGTGTTCAGTTGGTGGCTCTCCAACATTCAGCACTGGTAGCCAGTCATCCCATTGGGACAGCAGTTACCCGCTCTCCCCACTAGTGGCTCACTGCGTTCACACCGAGCCCCTGTCTAGTTTAGCTTTTACCCAGGAAGCTATTCTCACTGCATCTTGGGAAGGGCATGTAAAAATTTGGATGAGACCAGGTGAACCCGAAAGTAAATCCACCAACTCAGAAGCTCTGCCAGGTGCTAGTTTGAGGGACCAACTACCGCCGACAGGAAAAAGTTACTCAGAATCATTGAGGGCTCTTCTAGGTGCTAGTTCAAAGGAGCAACAGCCATCGACGGGCAAAGGTGGTGCCTTTAGTTATAAAGACTTCTCAAAAAGCAACCGATACCTTCCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

588

Amino Acids

62.68

Weight (kDa)

7.99

Isoelectric Point (pI)

40.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WDR55 PF24796 305 - 415 3e-06 WDR55
Beta-prop_WDR3_1st PF25173 311 - 452 2.5e-09 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 318 - 414 4.3e-09 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 321 - 415 2e-11 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 323 - 420 1.2e-12 WDR5 beta-propeller domain
Beta-prop_EML_2 PF23414 330 - 416 9.9e-08 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 735, 887, 1731
AasI GACNNNNNNGTC 1 cut(s) 100
AatII GACGTC 1 cut(s) 98
Acc16I TGCGCA 1 cut(s) 111
Acc36I ACCTGC 1 cut(s) 220
AccB1I GGYRCC 3 cut(s) 271, 722, 1718
AccBSI CCGCTC 1 cut(s) 1447
AccII CGCG 3 cut(s) 184, 320, 608
AccIII TCCGGA 2 cut(s) 994, 1120
AclWI GGATC 5 cut(s) 554, 567, 887, 900, 918
AcoI YGGCCR 1 cut(s) 185
AcsI RAATTY 1 cut(s) 1548
AcuI CTGAAG 1 cut(s) 269
AcyI GRCGYC 3 cut(s) 45, 95, 272
AfaI GTAC 8 cut(s) 5, 161, 212, 769, 802, 836, 1034, 1111
AfiI CCNNNNNNNGG 9 cut(s) 130, 153, 250, 287, 322, 630, 1459, 1637, 1706
AflIII ACRYGT 1 cut(s) 606
AgeI ACCGGT 1 cut(s) 863
AgsI TTSAA 3 cut(s) 142, 511, 1686
AhdI GACNNNNNGTC 1 cut(s) 477
AhlI ACTAGT 1 cut(s) 1456
AjnI CCWGG 4 cut(s) 264, 1506, 1561, 1603
AluBI AGCT 8 cut(s) 166, 247, 657, 715, 1199, 1499, 1514, 1598
AluI AGCT 8 cut(s) 166, 247, 657, 715, 1199, 1499, 1514, 1598
Alw21I GWGCWC 2 cut(s) 424, 659
Alw26I GTCTC 2 cut(s) 679, 1552
AlwI GGATC 5 cut(s) 554, 567, 887, 900, 918
AlwNI CAGNNNCTG 1 cut(s) 765
Ama87I CYCGRG 1 cut(s) 287
Aor13HI TCCGGA 2 cut(s) 994, 1120
AoxI GGCC 4 cut(s) 185, 257, 278, 456
ApaI GGGCCC 1 cut(s) 460
ApeKI GCWGC 4 cut(s) 24, 166, 199, 812
ApoI RAATTY 1 cut(s) 1548
ArsI GACNNNNNNTTYG 2 cut(s) 30, 62
AsiGI ACCGGT 1 cut(s) 863
Asp700I GAANNNNTTC 1 cut(s) 1515
AspLEI GCGC 6 cut(s) 29, 89, 112, 274, 315, 367
AspS9I GGNCC 7 cut(s) 258, 278, 283, 441, 456, 457, 1621
AsuC2I CCSGG 4 cut(s) 149, 276, 288, 289
AsuHPI GGTGA 4 cut(s) 878, 1217, 1251, 1577
AsuII TTCGAA 1 cut(s) 590
AsuNHI GCTAGC 1 cut(s) 238
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 3 cut(s) 283, 441, 1621
BaeGI GKGCMC 2 cut(s) 460, 727
BamHI GGATCC 2 cut(s) 559, 892
BanI GGYRCC 3 cut(s) 271, 722, 1718
BanII GRGCYC 4 cut(s) 460, 659, 1485, 1669
BarI GAAGNNNNNNTAC 2 cut(s) 1353, 1385
BbsI GAAGAC 1 cut(s) 185
Bbv12I GWGCWC 2 cut(s) 424, 659
BbvI GCAGC 4 cut(s) 36, 153, 186, 799
BccI CCATC 8 cut(s) 623, 818, 945, 992, 1022, 1118, 1265, 1708
BceAI ACGGC 2 cut(s) 58, 499
BcgI CGANNNNNNTGC 4 cut(s) 78, 112, 181, 215
BciT130I CCWGG 4 cut(s) 266, 1508, 1563, 1605
BcnI CCSGG 4 cut(s) 149, 276, 288, 289
BcoDI GTCTC 2 cut(s) 679, 1552
BcuI ACTAGT 1 cut(s) 1456
BfaI CTAG 7 cut(s) 239, 437, 1457, 1491, 1611, 1673, 1680
BfmI CTRYAG 1 cut(s) 1014
BfoI RGCGCY 3 cut(s) 30, 275, 368
BfuAI ACCTGC 1 cut(s) 220
Bme1390I CCNGG 8 cut(s) 149, 266, 276, 288, 289, 1508, 1563, 1605
Bme18I GGWCC 3 cut(s) 283, 441, 1621
BmeRI GACNNNNNGTC 1 cut(s) 477
BmeT110I CYCGRG 1 cut(s) 287
BmgT120I GGNCC 7 cut(s) 258, 278, 283, 441, 456, 457, 1621
BmrFI CCNGG 8 cut(s) 149, 266, 276, 288, 289, 1508, 1563, 1605
BmrI ACTGGG 2 cut(s) 112, 875
BmsI GCATC 1 cut(s) 1535
BmtI GCTAGC 1 cut(s) 242
BmuI ACTGGG 2 cut(s) 112, 875
BoxI GACNNNNGTC 1 cut(s) 439
BpiI GAAGAC 1 cut(s) 185
BpmI CTGGAG 2 cut(s) 681, 1164
Bpu14I TTCGAA 1 cut(s) 590
BpuMI CCSGG 4 cut(s) 149, 276, 288, 289
BsaBI GATNNNNATC 1 cut(s) 558
BsaHI GRCGYC 3 cut(s) 45, 95, 272
BsaI GGTCTC 1 cut(s) 1552
BsaJI CCNNGG 7 cut(s) 147, 275, 286, 287, 624, 1347, 1506
BsaWI WCCGGW 5 cut(s) 104, 595, 863, 994, 1120
BsaXI ACNNNNNCTCC 4 cut(s) 143, 173, 1433, 1463
Bsc4I CCNNNNNNNGG 9 cut(s) 130, 153, 250, 287, 322, 630, 1459, 1637, 1706
Bse118I RCCGGY 4 cut(s) 187, 280, 863, 1374
Bse1I ACTGG 8 cut(s) 118, 759, 771, 881, 927, 1147, 1414, 1417
Bse3DI GCAATG 1 cut(s) 802
Bse8I GATNNNNATC 1 cut(s) 558
BseAI TCCGGA 2 cut(s) 994, 1120
BseBI CCWGG 4 cut(s) 266, 1508, 1563, 1605
BseDI CCNNGG 7 cut(s) 147, 275, 286, 287, 624, 1347, 1506
BseGI GGATG 7 cut(s) 634, 1003, 1129, 1276, 1357, 1421, 1560
BseJI GATNNNNATC 1 cut(s) 558
BseLI CCNNNNNNNGG 9 cut(s) 130, 153, 250, 287, 322, 630, 1459, 1637, 1706
BseMI GCAATG 1 cut(s) 802
BseMII CTCAG 3 cut(s) 914, 1605, 1665
BseNI ACTGG 8 cut(s) 118, 759, 771, 881, 927, 1147, 1414, 1417
BseSI GKGCMC 2 cut(s) 460, 727
BseX3I CGGCCG 1 cut(s) 185
BseXI GCAGC 4 cut(s) 36, 153, 186, 799
BseYI CCCAGC 1 cut(s) 243
BsgI GTGCAG 1 cut(s) 152
Bsh1236I CGCG 3 cut(s) 184, 320, 608
Bsh1285I CGRYCG 1 cut(s) 188
BshFI GGCC 4 cut(s) 187, 259, 280, 458
BshNI GGYRCC 3 cut(s) 271, 722, 1718
BshTI ACCGGT 1 cut(s) 863
BsiEI CGRYCG 1 cut(s) 188
BsiHKAI GWGCWC 2 cut(s) 424, 659
BsiHKCI CYCGRG 1 cut(s) 287
BsiWI CGTACG 1 cut(s) 210
BslFI GGGAC 5 cut(s) 106, 269, 1015, 1445, 1634
BslI CCNNNNNNNGG 9 cut(s) 130, 153, 250, 287, 322, 630, 1459, 1637, 1706
BsmAI GTCTC 2 cut(s) 679, 1552
BsmFI GGGAC 5 cut(s) 106, 269, 1015, 1445, 1634
BsnI GGCC 4 cut(s) 187, 259, 280, 458
Bso31I GGTCTC 1 cut(s) 1552
BsoBI CYCGRG 1 cut(s) 287
Bsp119I TTCGAA 1 cut(s) 590
Bsp120I GGGCCC 1 cut(s) 456
Bsp1286I GDGCHC 6 cut(s) 424, 460, 659, 727, 1485, 1669
Bsp13I TCCGGA 2 cut(s) 994, 1120
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 5 cut(s) 538, 559, 892, 923, 1156
Bsp19I CCATGG 1 cut(s) 1347
BspANI GGCC 4 cut(s) 187, 259, 280, 458
BspCNI CTCAG 3 cut(s) 913, 1604, 1664
BspEI TCCGGA 2 cut(s) 994, 1120
BspFNI CGCG 3 cut(s) 184, 320, 608
BspMI ACCTGC 1 cut(s) 220
BspOI GCTAGC 1 cut(s) 242
BspPI GGATC 5 cut(s) 554, 567, 887, 900, 918
BspQI GCTCTTC 1 cut(s) 1674
BspT104I TTCGAA 1 cut(s) 590
BspT107I GGYRCC 3 cut(s) 271, 722, 1718
BspTNI GGTCTC 1 cut(s) 1552
BsrBI CCGCTC 1 cut(s) 1447
BsrDI GCAATG 1 cut(s) 802
BsrFI RCCGGY 4 cut(s) 187, 280, 863, 1374
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 8 cut(s) 118, 759, 771, 881, 927, 1147, 1414, 1417
BssAI RCCGGY 4 cut(s) 187, 280, 863, 1374
BssECI CCNNGG 7 cut(s) 147, 275, 286, 287, 624, 1347, 1506
BssMI GATC 5 cut(s) 538, 559, 892, 923, 1156
BssNI GRCGYC 3 cut(s) 45, 95, 272
BssT1I CCWWGG 2 cut(s) 624, 1347
Bst2UI CCWGG 4 cut(s) 266, 1508, 1563, 1605
Bst4CI ACNGT 3 cut(s) 17, 1018, 1168
Bst6I CTCTTC 2 cut(s) 1104, 1674
BstACI GRCGYC 3 cut(s) 45, 95, 272
BstAPI GCANNNNNTGC 1 cut(s) 640
BstAUI TGTACA 1 cut(s) 3
BstBI TTCGAA 1 cut(s) 590
BstC8I GCNNGC 4 cut(s) 31, 189, 240, 301
BstDEI CTNAG 4 cut(s) 368, 900, 1591, 1651
BstDSI CCRYGG 1 cut(s) 1347
BstENI CCTNNNNNAGG 1 cut(s) 128
BstF5I GGATG 7 cut(s) 634, 1003, 1129, 1276, 1357, 1421, 1560
BstFNI CGCG 3 cut(s) 184, 320, 608
BstH2I RGCGCY 3 cut(s) 30, 275, 368
BstHHI GCGC 6 cut(s) 29, 89, 112, 274, 315, 367
BstKTI GATC 5 cut(s) 541, 562, 895, 926, 1159
BstMAI GTCTC 2 cut(s) 679, 1552
BstMBI GATC 5 cut(s) 538, 559, 892, 923, 1156
BstMCI CGRYCG 1 cut(s) 188
BstMWI GCNNNNNNNGC 7 cut(s) 30, 163, 235, 244, 640, 818, 1413
BstNI CCWGG 4 cut(s) 266, 1508, 1563, 1605
BstNSI RCATGY 1 cut(s) 1544
BstPAI GACNNNNGTC 1 cut(s) 439
BstSCI CCNGG 8 cut(s) 147, 264, 274, 286, 287, 1506, 1561, 1603
BstSFI CTRYAG 1 cut(s) 1014
BstSLI GKGCMC 2 cut(s) 460, 727
BstUI CGCG 3 cut(s) 184, 320, 608
BstV1I GCAGC 4 cut(s) 36, 153, 186, 799
BstV2I GAAGAC 1 cut(s) 185
BstX2I RGATCY 3 cut(s) 559, 892, 923
BstYI RGATCY 3 cut(s) 559, 892, 923
BstZI CGGCCG 1 cut(s) 185
BsuRI GGCC 4 cut(s) 187, 259, 280, 458
BtgI CCRYGG 1 cut(s) 1347
BtgZI GCGATG 1 cut(s) 280
BtsCI GGATG 7 cut(s) 634, 1003, 1129, 1276, 1357, 1421, 1560
BtsI GCAGTG 2 cut(s) 1465, 1521
BtsIMutI CAGTG 4 cut(s) 915, 1407, 1465, 1521
BveI ACCTGC 1 cut(s) 220
Cac8I GCNNGC 4 cut(s) 31, 189, 240, 301
CaiI CAGNNNCTG 1 cut(s) 765
CfoI GCGC 6 cut(s) 29, 89, 112, 274, 315, 367
Cfr10I RCCGGY 4 cut(s) 187, 280, 863, 1374
Cfr13I GGNCC 7 cut(s) 258, 278, 283, 441, 456, 457, 1621
Cfr9I CCCGGG 1 cut(s) 287
CseI GACGC 2 cut(s) 34, 190
CsiI ACCWGGT 1 cut(s) 1561
Csp6I GTAC 8 cut(s) 4, 160, 211, 768, 801, 835, 1033, 1110
CspAI ACCGGT 1 cut(s) 863
CviAII CATG 9 cut(s) 7, 635, 780, 818, 917, 1177, 1221, 1348, 1541
CviQI GTAC 8 cut(s) 4, 160, 211, 768, 801, 835, 1033, 1110
DdeI CTNAG 4 cut(s) 368, 900, 1591, 1651
DinI GGCGCC 1 cut(s) 273
DpnI GATC 5 cut(s) 540, 561, 894, 925, 1158
DpnII GATC 5 cut(s) 538, 559, 892, 923, 1156
DrdI GACNNNNNNGTC 1 cut(s) 100
DriI GACNNNNNGTC 1 cut(s) 477
DseDI GACNNNNNNGTC 1 cut(s) 100
EaeI YGGCCR 1 cut(s) 185
EagI CGGCCG 1 cut(s) 185
Eam1104I CTCTTC 2 cut(s) 1104, 1674
Eam1105I GACNNNNNGTC 1 cut(s) 477
EarI CTCTTC 2 cut(s) 1104, 1674
Ecl136II GAGCTC 1 cut(s) 657
EclXI CGGCCG 1 cut(s) 185
Eco130I CCWWGG 2 cut(s) 624, 1347
Eco24I GRGCYC 4 cut(s) 460, 659, 1485, 1669
Eco31I GGTCTC 1 cut(s) 1552
Eco47I GGWCC 3 cut(s) 283, 441, 1621
Eco52I CGGCCG 1 cut(s) 185
Eco53kI GAGCTC 1 cut(s) 657
Eco57I CTGAAG 1 cut(s) 269
Eco88I CYCGRG 1 cut(s) 287
EcoICRI GAGCTC 1 cut(s) 657
EcoNI CCTNNNNNAGG 1 cut(s) 128
EcoO109I RGGNCCY 1 cut(s) 456
EcoRII CCWGG 4 cut(s) 264, 1506, 1561, 1603
EcoT14I CCWWGG 2 cut(s) 624, 1347
EcoT38I GRGCYC 4 cut(s) 460, 659, 1485, 1669
EgeI GGCGCC 1 cut(s) 273
EheI GGCGCC 1 cut(s) 273
ErhI CCWWGG 2 cut(s) 624, 1347
FaeI CATG 9 cut(s) 10, 638, 783, 821, 920, 1180, 1224, 1351, 1544
FalI AAGNNNNNCTT 4 cut(s) 1143, 1175, 1272, 1304
FaqI GGGAC 5 cut(s) 106, 269, 1015, 1445, 1634
FatI CATG 9 cut(s) 6, 634, 779, 817, 916, 1176, 1220, 1347, 1540
FauI CCCGC 2 cut(s) 296, 1452
FokI GGATG 7 cut(s) 641, 1010, 1136, 1283, 1364, 1408, 1567
FriOI GRGCYC 4 cut(s) 460, 659, 1485, 1669
FspBI CTAG 7 cut(s) 239, 437, 1457, 1491, 1611, 1673, 1680
FspI TGCGCA 1 cut(s) 111
GlaI GCGC 6 cut(s) 28, 88, 111, 273, 314, 366
GsaI CCCAGC 1 cut(s) 247
GsuI CTGGAG 2 cut(s) 681, 1164
HaeII RGCGCY 3 cut(s) 30, 275, 368
HaeIII GGCC 4 cut(s) 187, 259, 280, 458
HgaI GACGC 2 cut(s) 34, 190
HhaI GCGC 6 cut(s) 29, 89, 112, 274, 315, 367
Hin1I GRCGYC 3 cut(s) 45, 95, 272
Hin1II CATG 9 cut(s) 10, 638, 783, 821, 920, 1180, 1224, 1351, 1544
Hin6I GCGC 6 cut(s) 27, 87, 110, 272, 313, 365
HinP1I GCGC 6 cut(s) 27, 87, 110, 272, 313, 365
HindIII AAGCTT 1 cut(s) 713
HinfI GANTC 8 cut(s) 193, 478, 592, 614, 701, 1117, 1244, 1655
HphI GGTGA 4 cut(s) 878, 1217, 1251, 1577
Hpy166II GTNNAC 4 cut(s) 971, 988, 1474, 1568
Hpy188I TCNGA 5 cut(s) 175, 619, 1270, 1594, 1654
Hpy188III TCNNGA 6 cut(s) 142, 660, 948, 995, 1121, 1316
Hpy8I GTNNAC 4 cut(s) 971, 988, 1474, 1568
HpyAV CCTTC 6 cut(s) 244, 841, 959, 1183, 1202, 1529
HpyCH4III ACNGT 3 cut(s) 17, 1018, 1168
HpyCH4IV ACGT 2 cut(s) 56, 95
HpyCH4V TGCA 7 cut(s) 169, 577, 697, 730, 779, 920, 1526
HpyF10VI GCNNNNNNNGC 7 cut(s) 30, 163, 235, 244, 640, 818, 1413
HpyF3I CTNAG 4 cut(s) 368, 900, 1591, 1651
HpySE526I ACGT 2 cut(s) 56, 95
Hsp92I GRCGYC 3 cut(s) 45, 95, 272
Hsp92II CATG 9 cut(s) 10, 638, 783, 821, 920, 1180, 1224, 1351, 1544
HspAI GCGC 6 cut(s) 27, 87, 110, 272, 313, 365
KasI GGCGCC 1 cut(s) 271
Kpn2I TCCGGA 2 cut(s) 994, 1120
KroI GCCGGC 1 cut(s) 187
KroNI GCCGGC 1 cut(s) 189
Kzo9I GATC 5 cut(s) 538, 559, 892, 923, 1156
LguI GCTCTTC 1 cut(s) 1674
LmnI GCTCC 3 cut(s) 357, 1173, 1690
Lsp1109I GCAGC 4 cut(s) 36, 153, 186, 799
LweI GCATC 1 cut(s) 1535
MabI ACCWGGT 1 cut(s) 1561
MaeI CTAG 7 cut(s) 239, 437, 1457, 1491, 1611, 1673, 1680
MaeII ACGT 2 cut(s) 56, 95
MaeIII GTNAC 7 cut(s) 17, 232, 479, 602, 1162, 1439, 1646
MalI GATC 5 cut(s) 540, 561, 894, 925, 1158
MbiI CCGCTC 1 cut(s) 1447
MboI GATC 5 cut(s) 538, 559, 892, 923, 1156
MboII GAAGA 7 cut(s) 190, 721, 1091, 1163, 1193, 1292, 1661
MfeI CAATTG 1 cut(s) 692
MflI RGATCY 3 cut(s) 559, 892, 923
MhlI GDGCHC 6 cut(s) 424, 460, 659, 727, 1485, 1669
MluCI AATT 5 cut(s) 544, 586, 692, 942, 1548
MluI ACGCGT 1 cut(s) 606
Mly113I GGCGCC 1 cut(s) 272
MlyI GAGTC 3 cut(s) 487, 1126, 1238
MmeI TCCRAC 2 cut(s) 496, 1421
MnlI CCTC 9 cut(s) 145, 249, 321, 425, 688, 736, 1139, 1611, 1656
MroI TCCGGA 2 cut(s) 994, 1120
MroNI GCCGGC 1 cut(s) 187
MroXI GAANNNNTTC 1 cut(s) 1515
MseI TTAA 2 cut(s) 567, 753
MslI CAYNNNNRTG 2 cut(s) 822, 960
MspA1I CMGCKG 2 cut(s) 247, 303
MspR9I CCNGG 8 cut(s) 149, 266, 276, 288, 289, 1508, 1563, 1605
MunI CAATTG 1 cut(s) 692
MvaI CCWGG 4 cut(s) 266, 1508, 1563, 1605
MvnI CGCG 3 cut(s) 184, 320, 608
MwoI GCNNNNNNNGC 7 cut(s) 30, 163, 235, 244, 640, 818, 1413
NaeI GCCGGC 1 cut(s) 189
NarI GGCGCC 1 cut(s) 272
NciI CCSGG 4 cut(s) 149, 276, 288, 289
NcoI CCATGG 1 cut(s) 1347
NdeII GATC 5 cut(s) 538, 559, 892, 923, 1156
NgoMIV GCCGGC 1 cut(s) 187
NheI GCTAGC 1 cut(s) 238
NlaIII CATG 9 cut(s) 10, 638, 783, 821, 920, 1180, 1224, 1351, 1544
NmuCI GTSAC 3 cut(s) 232, 479, 602
NsbI TGCGCA 1 cut(s) 111
NspI RCATGY 1 cut(s) 1544
NspV TTCGAA 1 cut(s) 590
PciSI GCTCTTC 1 cut(s) 1674
PcsI WCGNNNNNNNCGW 7 cut(s) 62, 71, 74, 89, 98, 188, 473
PdiI GCCGGC 1 cut(s) 189
PdmI GAANNNNTTC 1 cut(s) 1515
PfeI GAWTC 5 cut(s) 193, 592, 614, 701, 1655
Pfl23II CGTACG 1 cut(s) 210
PflFI GACNNNGTC 1 cut(s) 97
PinAI ACCGGT 1 cut(s) 863
PleI GAGTC 3 cut(s) 486, 1125, 1238
PluTI GGCGCC 1 cut(s) 275
PpsI GAGTC 3 cut(s) 486, 1125, 1238
PshAI GACNNNNGTC 1 cut(s) 439
PsiI TTATAA 3 cut(s) 735, 887, 1731
Psp124BI GAGCTC 1 cut(s) 659
Psp6I CCWGG 4 cut(s) 264, 1506, 1561, 1603
PspFI CCCAGC 1 cut(s) 243
PspGI CCWGG 4 cut(s) 264, 1506, 1561, 1603
PspLI CGTACG 1 cut(s) 210
PspOMI GGGCCC 1 cut(s) 456
PspPI GGNCC 7 cut(s) 258, 278, 283, 441, 456, 457, 1621
PsrI GAACNNNNNNTAC 1 cut(s) 34
PstNI CAGNNNCTG 1 cut(s) 765
PsuI RGATCY 3 cut(s) 559, 892, 923
PsyI GACNNNGTC 1 cut(s) 97
PvuII CAGCTG 1 cut(s) 247
RsaI GTAC 8 cut(s) 5, 161, 212, 769, 802, 836, 1034, 1111
RsaNI GTAC 8 cut(s) 4, 160, 211, 768, 801, 835, 1033, 1110
RseI CAYNNNNRTG 2 cut(s) 822, 960
SacI GAGCTC 1 cut(s) 659
SapI GCTCTTC 1 cut(s) 1674
SaqAI TTAA 2 cut(s) 567, 753
Sau3AI GATC 5 cut(s) 538, 559, 892, 923, 1156
Sau96I GGNCC 7 cut(s) 258, 278, 283, 441, 456, 457, 1621
SchI GAGTC 3 cut(s) 487, 1126, 1238
ScrFI CCNGG 8 cut(s) 149, 266, 276, 288, 289, 1508, 1563, 1605
SduI GDGCHC 6 cut(s) 424, 460, 659, 727, 1485, 1669
SexAI ACCWGGT 1 cut(s) 1561
SfaNI GCATC 1 cut(s) 1535
SfcI CTRYAG 1 cut(s) 1014
SfoI GGCGCC 1 cut(s) 273
SfuI TTCGAA 1 cut(s) 590
SgrAI CRCCGGYG 1 cut(s) 1374
SinI GGWCC 3 cut(s) 283, 441, 1621
SmaI CCCGGG 1 cut(s) 289
SmiMI CAYNNNNRTG 2 cut(s) 822, 960
SpeI ACTAGT 1 cut(s) 1456
Sse9I AATT 5 cut(s) 544, 586, 692, 942, 1548
SspDI GGCGCC 1 cut(s) 271
SspMI CTAG 7 cut(s) 239, 437, 1457, 1491, 1611, 1673, 1680
SstI GAGCTC 1 cut(s) 659
StyD4I CCNGG 8 cut(s) 147, 264, 274, 286, 287, 1506, 1561, 1603
StyI CCWWGG 2 cut(s) 624, 1347
TaaI ACNGT 3 cut(s) 17, 1018, 1168
TaiI ACGT 2 cut(s) 59, 98
TaqI TCGA 3 cut(s) 476, 590, 1703
TasI AATT 5 cut(s) 544, 586, 692, 942, 1548
TatI WGTACW 2 cut(s) 3, 1109
TauI GCSGC 7 cut(s) 74, 187, 272, 323, 326, 1080, 1369
TfiI GAWTC 5 cut(s) 193, 592, 614, 701, 1655
Tru1I TTAA 2 cut(s) 567, 753
Tru9I TTAA 2 cut(s) 567, 753
TscAI CASTG 4 cut(s) 915, 1414, 1472, 1528
TseFI GTSAC 3 cut(s) 232, 479, 602
TseI GCWGC 4 cut(s) 24, 166, 199, 812
Tsp45I GTSAC 3 cut(s) 232, 479, 602
TspDTI ATGAA 1 cut(s) 23
TspGWI ACGGA 1 cut(s) 1752
TspMI CCCGGG 1 cut(s) 287
TspRI CASTG 4 cut(s) 915, 1414, 1472, 1528
Tth111I GACNNNGTC 1 cut(s) 97
VpaK11BI GGWCC 3 cut(s) 283, 441, 1621
XagI CCTNNNNNAGG 1 cut(s) 128
XapI RAATTY 1 cut(s) 1548
XceI RCATGY 1 cut(s) 1544
XcmI CCANNNNNNNNNTGG 1 cut(s) 948
XmaI CCCGGG 1 cut(s) 287
XmnI GAANNNNTTC 1 cut(s) 1515
XspI CTAG 7 cut(s) 239, 437, 1457, 1491, 1611, 1673, 1680
ZraI GACGTC 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.