Rorug05G0324500

WD repeat-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0007846
Physical Location & Seq
Reverse (-)
38943188 .. 38944087
900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0324500.1

Sequence Viewer

Length: 555 bp
ATGAGCAGCCGGGAGGTGAAATTTAGCTGGACCTCCGCCCTCATCGGCGCCGCTTCGGCCCTAGCCACCACCTCTCTTCTCTCCGCTAAGCCCAAGGACCCCACCTTCGACCTCATCTCCATCGACCTCACCTCCTTCAAGCTCAACCTCCCTCTCCTCGACGCCGGCCTCGTCCTCACCGTCCACATCACCAACCCCAACATCACCCCCATCCACTACTCCTCCACCACCATGTCCATCTTCTACAAAGGCTCCCTCCTCGGCTCCGCCGACATCAAGGCCGGCTCCCAGTCCGCCAAGTCCTGCAAGCTCCTCCGCCTCCCGGCCCGCCTCGACGGGCTCCAGCTCGCCCACCACGCCGCCAGCTTCGTCTCCGACGTCGCCAAGCGCCAGATGGTGCTCGACGCCGCCGTCGATATTGCCGGCGCCGCCAAGGTTCTGTGGTGGGAGCATAAGTTTGTGGTGCACGTGGACAGTCACATCACCGTTGATCCTCTGTTTCTTGACGTCATTGAGCAGGAAAACAAGTCCGATTTGGAGATTTTTGTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

184

Amino Acids

19.91

Weight (kDa)

6.36

Isoelectric Point (pI)

45.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 60 - 153 9.2e-11 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 410
AatII GACGTC 2 cut(s) 381, 510
AccB1I GGYRCC 2 cut(s) 47, 425
AclWI GGATC 1 cut(s) 485
AcsI RAATTY 1 cut(s) 20
AcvI CACGTG 1 cut(s) 469
AcyI GRCGYC 6 cut(s) 48, 162, 378, 405, 426, 507
AfiI CCNNNNNNNGG 1 cut(s) 322
AgsI TTSAA 1 cut(s) 139
AluBI AGCT 5 cut(s) 27, 142, 310, 346, 366
AluI AGCT 5 cut(s) 27, 142, 310, 346, 366
Alw21I GWGCWC 2 cut(s) 402, 468
Alw26I GTCTC 1 cut(s) 376
Alw44I GTGCAC 1 cut(s) 464
AlwI GGATC 1 cut(s) 485
AoxI GGCC 4 cut(s) 57, 166, 279, 324
ApaLI GTGCAC 1 cut(s) 464
ApeKI GCWGC 1 cut(s) 6
ApoI RAATTY 1 cut(s) 20
ArsI GACNNNNNNTTYG 2 cut(s) 89, 121
AspLEI GCGC 3 cut(s) 50, 390, 428
AspS9I GGNCC 4 cut(s) 30, 58, 97, 325
AsuC2I CCSGG 2 cut(s) 11, 323
AsuHPI GGTGA 6 cut(s) 28, 121, 169, 181, 196, 475
AvaII GGWCC 2 cut(s) 30, 97
BaeGI GKGCMC 1 cut(s) 468
BanI GGYRCC 2 cut(s) 47, 425
BanII GRGCYC 1 cut(s) 342
BbrPI CACGTG 1 cut(s) 469
Bbv12I GWGCWC 2 cut(s) 402, 468
BbvI GCAGC 1 cut(s) 18
BccI CCATC 4 cut(s) 128, 218, 245, 388
BceAI ACGGC 1 cut(s) 395
BcnI CCSGG 2 cut(s) 11, 323
BcoDI GTCTC 1 cut(s) 376
BfaI CTAG 1 cut(s) 62
BfoI RGCGCY 3 cut(s) 51, 391, 429
BglI GCCNNNNNGGC 1 cut(s) 56
BisI GCNGC 5 cut(s) 7, 51, 360, 408, 429
BlpI GCTNAGC 1 cut(s) 87
BlsI GCNGC 5 cut(s) 8, 52, 361, 409, 430
Bme1390I CCNGG 2 cut(s) 11, 323
Bme18I GGWCC 2 cut(s) 30, 97
BmgT120I GGNCC 4 cut(s) 30, 58, 97, 325
BmiI GGNNCC 7 cut(s) 49, 99, 253, 265, 286, 341, 427
BmrFI CCNGG 2 cut(s) 11, 323
BmrI ACTGGG 1 cut(s) 283
BmuI ACTGGG 1 cut(s) 283
BpmI CTGGAG 1 cut(s) 326
Bpu1102I GCTNAGC 1 cut(s) 87
BpuMI CCSGG 2 cut(s) 11, 323
BsaAI YACGTR 1 cut(s) 469
BsaHI GRCGYC 6 cut(s) 48, 162, 378, 405, 426, 507
BsaJI CCNNGG 3 cut(s) 93, 259, 432
Bsc4I CCNNNNNNNGG 1 cut(s) 322
Bse118I RCCGGY 3 cut(s) 164, 281, 422
Bse1I ACTGG 1 cut(s) 289
BseDI CCNNGG 3 cut(s) 93, 259, 432
BseGI GGATG 1 cut(s) 210
BseLI CCNNNNNNNGG 1 cut(s) 322
BseNI ACTGG 1 cut(s) 289
BseRI GAGGAG 4 cut(s) 146, 211, 248, 302
BseSI GKGCMC 1 cut(s) 468
BseXI GCAGC 1 cut(s) 18
BshFI GGCC 4 cut(s) 59, 168, 281, 326
BshNI GGYRCC 2 cut(s) 47, 425
BsiHKAI GWGCWC 2 cut(s) 402, 468
BsiSI CCGG 5 cut(s) 10, 165, 282, 323, 423
BslI CCNNNNNNNGG 1 cut(s) 322
BsmAI GTCTC 1 cut(s) 376
BsmBI CGTCTC 1 cut(s) 376
BsnI GGCC 4 cut(s) 59, 168, 281, 326
Bsp1286I GDGCHC 3 cut(s) 342, 402, 468
Bsp143I GATC 1 cut(s) 490
Bsp1720I GCTNAGC 1 cut(s) 87
BspANI GGCC 4 cut(s) 59, 168, 281, 326
BspLI GGNNCC 7 cut(s) 49, 99, 253, 265, 286, 341, 427
BspPI GGATC 1 cut(s) 485
BspT107I GGYRCC 2 cut(s) 47, 425
BsrFI RCCGGY 3 cut(s) 164, 281, 422
BsrI ACTGG 1 cut(s) 289
BssAI RCCGGY 3 cut(s) 164, 281, 422
BssECI CCNNGG 3 cut(s) 93, 259, 432
BssMI GATC 1 cut(s) 490
BssNI GRCGYC 6 cut(s) 48, 162, 378, 405, 426, 507
BssT1I CCWWGG 2 cut(s) 93, 432
Bst4CI ACNGT 3 cut(s) 181, 476, 487
Bst6I CTCTTC 1 cut(s) 81
BstACI GRCGYC 6 cut(s) 48, 162, 378, 405, 426, 507
BstBAI YACGTR 1 cut(s) 469
BstC8I GCNNGC 7 cut(s) 166, 283, 308, 328, 348, 364, 424
BstDEI CTNAG 1 cut(s) 87
BstF5I GGATG 1 cut(s) 210
BstH2I RGCGCY 3 cut(s) 51, 391, 429
BstHHI GCGC 3 cut(s) 50, 390, 428
BstKTI GATC 1 cut(s) 493
BstMAI GTCTC 1 cut(s) 376
BstMBI GATC 1 cut(s) 490
BstMWI GCNNNNNNNGC 3 cut(s) 56, 356, 428
BstSCI CCNGG 2 cut(s) 9, 321
BstSLI GKGCMC 1 cut(s) 468
BstV1I GCAGC 1 cut(s) 18
BsuRI GGCC 4 cut(s) 59, 168, 281, 326
BtsCI GGATG 1 cut(s) 210
Cac8I GCNNGC 7 cut(s) 166, 283, 308, 328, 348, 364, 424
CfoI GCGC 3 cut(s) 50, 390, 428
Cfr10I RCCGGY 3 cut(s) 164, 281, 422
Cfr13I GGNCC 4 cut(s) 30, 58, 97, 325
CseI GACGC 2 cut(s) 170, 413
CviAII CATG 1 cut(s) 232
DdeI CTNAG 1 cut(s) 87
DinI GGCGCC 2 cut(s) 49, 427
DpnI GATC 1 cut(s) 492
DpnII GATC 1 cut(s) 490
DrdI GACNNNNNNGTC 1 cut(s) 410
DseDI GACNNNNNNGTC 1 cut(s) 410
Eam1104I CTCTTC 1 cut(s) 81
EarI CTCTTC 1 cut(s) 81
EciI GGCGGA 4 cut(s) 25, 256, 283, 305
Eco130I CCWWGG 2 cut(s) 93, 432
Eco24I GRGCYC 1 cut(s) 342
Eco47I GGWCC 2 cut(s) 30, 97
Eco72I CACGTG 1 cut(s) 469
EcoO109I RGGNCCY 1 cut(s) 97
EcoT14I CCWWGG 2 cut(s) 93, 432
EcoT38I GRGCYC 1 cut(s) 342
EgeI GGCGCC 2 cut(s) 49, 427
EheI GGCGCC 2 cut(s) 49, 427
ErhI CCWWGG 2 cut(s) 93, 432
Esp3I CGTCTC 1 cut(s) 376
FaeI CATG 1 cut(s) 235
FaiI YATR 2 cut(s) 233, 453
FatI CATG 1 cut(s) 231
FauI CCCGC 1 cut(s) 335
Fnu4HI GCNGC 5 cut(s) 7, 51, 360, 408, 429
FokI GGATG 1 cut(s) 197
FriOI GRGCYC 1 cut(s) 342
Fsp4HI GCNGC 5 cut(s) 7, 51, 360, 408, 429
FspBI CTAG 1 cut(s) 62
GlaI GCGC 3 cut(s) 49, 389, 427
GluI GCNGC 5 cut(s) 7, 51, 360, 408, 429
GsuI CTGGAG 1 cut(s) 326
HaeII RGCGCY 3 cut(s) 51, 391, 429
HaeIII GGCC 4 cut(s) 59, 168, 281, 326
HapII CCGG 5 cut(s) 10, 165, 282, 323, 423
HgaI GACGC 2 cut(s) 170, 413
HhaI GCGC 3 cut(s) 50, 390, 428
Hin1I GRCGYC 6 cut(s) 48, 162, 378, 405, 426, 507
Hin1II CATG 1 cut(s) 235
Hin6I GCGC 3 cut(s) 48, 388, 426
HinP1I GCGC 3 cut(s) 48, 388, 426
HpaII CCGG 5 cut(s) 10, 165, 282, 323, 423
HphI GGTGA 6 cut(s) 28, 121, 169, 181, 196, 475
Hpy166II GTNNAC 3 cut(s) 184, 466, 472
Hpy188I TCNGA 2 cut(s) 376, 532
Hpy188III TCNNGA 1 cut(s) 503
Hpy8I GTNNAC 3 cut(s) 184, 466, 472
Hpy99I CGWCG 6 cut(s) 164, 338, 380, 383, 407, 416
HpyAV CCTTC 2 cut(s) 115, 145
HpyCH4III ACNGT 3 cut(s) 181, 476, 487
HpyCH4IV ACGT 3 cut(s) 378, 468, 507
HpyCH4V TGCA 2 cut(s) 306, 466
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 356, 428
HpyF3I CTNAG 1 cut(s) 87
HpySE526I ACGT 3 cut(s) 378, 468, 507
Hsp92I GRCGYC 6 cut(s) 48, 162, 378, 405, 426, 507
Hsp92II CATG 1 cut(s) 235
HspAI GCGC 3 cut(s) 48, 388, 426
KasI GGCGCC 2 cut(s) 47, 425
KroI GCCGGC 3 cut(s) 164, 281, 422
KroNI GCCGGC 3 cut(s) 166, 283, 424
Kzo9I GATC 1 cut(s) 490
LmnI GCTCC 6 cut(s) 257, 269, 290, 315, 345, 448
Lsp1109I GCAGC 1 cut(s) 18
MaeI CTAG 1 cut(s) 62
MaeII ACGT 3 cut(s) 378, 468, 507
MaeIII GTNAC 1 cut(s) 476
MalI GATC 1 cut(s) 492
MboI GATC 1 cut(s) 490
MboII GAAGA 2 cut(s) 68, 232
MhlI GDGCHC 3 cut(s) 342, 402, 468
MluCI AATT 1 cut(s) 20
Mly113I GGCGCC 2 cut(s) 48, 426
MmeI TCCRAC 1 cut(s) 399
MroNI GCCGGC 3 cut(s) 164, 281, 422
MseI TTAA 1 cut(s) 553
MslI CAYNNNNRTG 1 cut(s) 230
MspI CCGG 5 cut(s) 10, 165, 282, 323, 423
MspR9I CCNGG 2 cut(s) 11, 323
MwoI GCNNNNNNNGC 3 cut(s) 56, 356, 428
NaeI GCCGGC 3 cut(s) 166, 283, 424
NarI GGCGCC 2 cut(s) 48, 426
NciI CCSGG 2 cut(s) 11, 323
NdeII GATC 1 cut(s) 490
NgoMIV GCCGGC 3 cut(s) 164, 281, 422
NlaIII CATG 1 cut(s) 235
NlaIV GGNNCC 7 cut(s) 49, 99, 253, 265, 286, 341, 427
NmeAIII GCCGAG 1 cut(s) 240
NmuCI GTSAC 1 cut(s) 476
PcsI WCGNNNNNNNCGW 6 cut(s) 168, 177, 267, 375, 408, 411
PdiI GCCGGC 3 cut(s) 166, 283, 424
PkrI GCNGC 5 cut(s) 8, 52, 361, 409, 430
PluTI GGCGCC 2 cut(s) 51, 429
PmaCI CACGTG 1 cut(s) 469
PmlI CACGTG 1 cut(s) 469
Ppu21I YACGTR 1 cut(s) 469
PpuMI RGGWCCY 1 cut(s) 97
Psp5II RGGWCCY 1 cut(s) 97
PspCI CACGTG 1 cut(s) 469
PspN4I GGNNCC 7 cut(s) 49, 99, 253, 265, 286, 341, 427
PspPI GGNCC 4 cut(s) 30, 58, 97, 325
PspPPI RGGWCCY 1 cut(s) 97
RseI CAYNNNNRTG 1 cut(s) 230
SaqAI TTAA 1 cut(s) 553
SatI GCNGC 5 cut(s) 7, 51, 360, 408, 429
Sau3AI GATC 1 cut(s) 490
Sau96I GGNCC 4 cut(s) 30, 58, 97, 325
ScrFI CCNGG 2 cut(s) 11, 323
SduI GDGCHC 3 cut(s) 342, 402, 468
SfoI GGCGCC 2 cut(s) 49, 427
SinI GGWCC 2 cut(s) 30, 97
SmiMI CAYNNNNRTG 1 cut(s) 230
Sse9I AATT 1 cut(s) 20
SspDI GGCGCC 2 cut(s) 47, 425
SspMI CTAG 1 cut(s) 62
StyD4I CCNGG 2 cut(s) 9, 321
StyI CCWWGG 2 cut(s) 93, 432
TaaI ACNGT 3 cut(s) 181, 476, 487
TaiI ACGT 3 cut(s) 381, 471, 510
TaqI TCGA 6 cut(s) 108, 123, 159, 333, 402, 414
TasI AATT 1 cut(s) 20
TauI GCSGC 4 cut(s) 53, 362, 410, 431
Tru1I TTAA 1 cut(s) 553
Tru9I TTAA 1 cut(s) 553
TseFI GTSAC 1 cut(s) 476
TseI GCWGC 1 cut(s) 6
Tsp45I GTSAC 1 cut(s) 476
VneI GTGCAC 1 cut(s) 464
VpaK11BI GGWCC 2 cut(s) 30, 97
XapI RAATTY 1 cut(s) 20
XcmI CCANNNNNNNNNTGG 1 cut(s) 391
XspI CTAG 1 cut(s) 62
ZraI GACGTC 2 cut(s) 379, 508
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.